Resumo Este estudo objetivou quantificar minerais presentes em genótipos de batata-doce de polpa colorida, selecionados e cultivados pela Embrapa (Empresa Brasileira de Pesquisa Agropecuária), no sul do Rio Grande do Sul, bem como verificar o percentual de adequação de consumo em grupos de risco nutricional. Genótipos de batata-doce cultivados no Banco Ativo de Germoplasma ‒ polpa branca (Rubissol, ILS 12, ILS 10, ILS 24); polpa creme (Cuia, ILS 03, ILS 44); polpa amarela/laranja (Amélia e Beauregard); polpa roxa (ILS 56, ILS 16 e ILS 71) ‒ foram analisados em triplicata. Os minerais foram quantificados por espectrometria de absorção atômica e os resultados foram expressos em miligrama do mineral correspondente a 100 g-1 de amostra úmida. Cálculos dos percentuais de adequação da ingestão diária recomendada de cada genótipo para grupos de risco (crianças entre 4 e 8 anos e gestantes entre 19 e 30 anos), com base no consumo de uma porção média de 200 g diários, foram realizados. As batatas-doces apresentaram grande variação na quantidade de minerais entre os genótipos e o potássio foi o mais abundante em todos estes, com destaque para os genótipos ILS 44, ILS 56 e ILS 71. Os genótipos de coloração roxa se sobressaíram na quantidade de potássio e os de coloração creme, na quantidade de fósforo. O consumo de uma porção média de 200 g de qualquer uma das batatas-doces forneceria 28% da necessidade de magnésio a crianças de 4 a 8 anos, e em torno de 20% das necessidades diárias de magnésio e 10% de potássio para gestantes.

Abstract The objective of this work was to quantify the minerals present in coloured pulp sweet potato genotypes, selected and cultivated by Embrapa (Empresa Brasileira de Pesquisa Agropecuária), in the southern region of Rio Grande do Sul, Brazil, as well as checking their percentage adequacy for consumption by groups at nutritional risk. The following sweet potato genotypes grown in the Active Germplasm Bank were analysed in triplicate: white pulp (Rubissol, ILS 12, ILS 10 and ILS 24); cream pulp (Cuia, ILS 03 and ILS 44); yellow/orange pulp (Amélia and Beauregard); and purple pulp (ILS 56, ILS 16 and ILS 71). The minerals were quantified by atomic absorption spectrometry and expressed as mg of the corresponding mineral per 100 g-1 of fresh weight. The percentages of adequacy of the recommended daily intake of each genotype for risk groups (children between 4 and 8 years old and pregnant women between 19 and 30 years old) based on the consumption of an average portion of 200 g per day, were calculated. The sweet potatoes showed great variation in the minerals amongst the genotypes, and potassium was the most abundant in all of them, especially in the genotypes ILS 44, ILS 56 and ILS 71. The purple coloured genotypes stood out for their potassium contents and the cream coloured genotypes for their phosphorus contents. The consumption of a portion about 200 g of any sweet potatoes would provide 28% of the magnesium requirement for children, aged 4 to 8 years, and about 20% of the daily requirement of magnesium and 10% of potassium, for pregnant women.

RESUMO: A batata-doce ( Ipomoea batatas (L.) Lam) é uma das raízes mais populares e antigas do Brasil, podendo ser consumida cozida, assada ou na forma de doces. A sua cocção pode levar à transformações físico-químicas alterando as propriedades nutricionais. O objetivo deste trabalho foi avaliar as características físico-químicas, compostos bioativos e atividade antioxidante de doze genótipos de batata-doce, de coloração de polpa variada, na forma in natura e assada. Foram determinados sólidos solúveis totais, acidez, açúcares, carotenoides, antocianinas, compostos fenólicos e atividade antioxidante nos seguintes genótipos de batatas-doces: polpa creme (Rubissol, Cuia, ILS03, ILS10, ILS12, ILS24 e ILS44); polpa laranja (Amélia e Beauregard) e polpa roxa (ILS56, ILS16 e ILS71). Quanto aos resultados foi observada ampla variação entre os genótipos de batata-doce, para todos os parâmetros analisados, em ambas as formas de preparo. A atividade antioxidante foi um parâmetro que demonstrou grande variação entre os genótipos, de 210,29 a 7870,57µg de equivalente trolox/g nos genótipos in natura e de 673,26 a 17306,22µg de equivalente trolox/g nos genótipos assados. Foi observado que em batatas-doces assadas os sólidos solúveis, acidez, açúcares e os compostos bioativos, com exceção dos carotenoides, tenderam a ser concentrados, elevando também a atividade antioxidante total. Em conclusão, o genótipo e a coloração da batata-doce foram parâmetros que exerceram influência sob a sua composição química. Cultivares como Amélia e Beauregard se destacaram pela quantidade de sólidos solúveis totais e carotenoides, respectivamente. Como fonte de antocianinas, as seleções ILS 16 e ILS 56 são recomendadas. O processo térmico influenciou a concentração de compostos antioxidantes e alterou algumas características físico-químicas.

ABSTRACT: Sweet potato ( Ipomoea batatas (L.) Lam) is one of the most popular and ancient roots of Brazil and it can be consumed at different forms such as boiled, roasted or as sweets. Its cooking can lead to physicochemical transformations altering the nutritional properties. The objective of this study was to evaluate the physicochemical characteristics, bioactive compounds and antioxidant capacity of twelve sweet potato genotypes of varying pulp color in natura and roasted. Soluble solids, acidity, sugars, carotenoids, anthocyanins, phenolic compounds and antioxidant capacity were analyzed in the following sweet potatoes genotypes: cream pulp (Rubissol, Cuia, ILS03, ILS10, ILS12, ILS24 and ILS44); orange pulp (Amelia and Beauregard); and purple pulp (ILS56, ILS16 and ILS71). According to the results, it was observed a wide variation among the sweet potato genotypes for all analyzed parameters, in both preparation forms. The antioxidant capacity was a parameter with wide variation among genotypes, 210.29 to 7870.57µg trolox equivalent/g in in natura form and 673.26 to 17306.22µg trolox equivalent/g in roasted form. Soluble solids, acidity, sugars and bioactive compounds, with the exception of carotenoids, tended to be concentrated, also increases the total antioxidant capacity, in roasted sweet potatoes. In conclusion, genotype and the color of sweet potatoes were parameters that had an influence on its chemical composition. Cultivars such as Amelia and Beauregard stood out by the amounts of total soluble solids and carotenoids, respectively. The selections ILS 16 and ILS 56 are recommended as sources of anthocyanins. Thermal process influenced the concentration of antioxidant compounds and changed some physicochemical characteristics.

Abstract Pitted keratolysis is a skin disorder that affects the stratum corneum of the plantar surface and is caused by Gram-positive bacteria. A 30-year-old male presented with small punched-out lesions on the plantar surface. A superficial shaving was carried out for scanning electron microscopy. Hypokeratosis was noted on the plantar skin and in the acrosyringium, where the normal elimination of corneocytes was not seen. At higher magnification (x 3,500) bacteria were easily found on the surface and the described transversal bacterial septation was observed.

AbstractSuperficial white onychomycosis is characterized by opaque, friable, whitish superficial spots on the nail plate. We examined an affected halux nail of a 20-year-old male patient with scanning electron microscopy. The mycological examination isolated Trichophyton mentagrophytes. Abundant hyphae with the formation of arthrospores were found on the nail's surface, forming small fungal colonies. These findings showed the great capacity for dissemination of this form of onychomycosis.

A epidermólise bolhosa albo-papulóide de Pasini é uma variante rara da forma generalizada de epidermólise bolhosa distrófica dominante. Uma paciente de 30 anos apresenta desde a infância pápulas e bolhas disseminadas. A microscopia óptica de uma bolha demonstrou clivagem dermo-epidérmica; além disso áreas focais de desprendimento dermo-epidérmico foram encontradas. A microscopia eletrônica de transmissão identificou a clivagem abaixo da lâmina densa. É importante que se reconheça essa variante de epidermólise bolhosa, já que o aspecto clínico predominante são pápulas disseminadas e não bolhas como nas outras formas de epidermólise bolhosa.

Pasini's albopapuloid epidermolysis bullosa is a very rare subtype of generalized dystrophic dominant epidermolyis bullosa. A 30 year-old white female patient presented since her childhood disseminated small blisters and papules. Light microscopy of a blister showed dermal-epidermal cleavage; moreover, focal areas of dermal-epidermal splitting were also observed. Transmission electron microscopy also identified focal areas of cleavage, which were seen below the lamina densa. It is important to recognize this condition as a variant of epidermolysis bullosa, since the most important cutaneous findings are generalized papules and not blisters and erosions as in other forms of epidermolysis bullosa.

OBJECTIVE: To examine the epidermis in induced phytophotodermatitis using transmission electron microscopy in order to detect histologic changes even before lesions are visible by light microscopy. INTRODUCTION: In the first six hours after the experimental induction of phytophotodermatitis, no changes are detectable by light microscopy. Only after 24 hours can keratinocyte necrosis and epidermal vacuolization be detected histologically, and blisters form by 48 hours. METHODS: The dorsum of four adult rats (Rattus norvegicus) was manually epilated. After painting the right half of the rat with the peel juice of Tahiti lemon, they were exposed to sunlight for eight minutes under general anesthesia. The left side was used as the control and exposed to sunlight only. Biopsies were performed immediately after photoinduction and one and two hours later, and the tissue was analyzed by transmission electron microscopy. RESULTS: No histological changes were seen on the control side. Immediately after induction, vacuolization in keratinocytes was observed. After one hour, desmosomal changes were also observed in addition to vacuolization. Keratin filaments were not attached to the desmosomal plaque. Free desmosomes and membrane ruptures were also seen. At two hours after induction, similar changes were found, and granular degeneration of keratin was also observed. DISCUSSION: The interaction of sunlight and psoralens generates a photoproduct that damages keratinocyte proteins, leading to keratinocyte necrosis and blister formation. CONCLUSIONS: Transmission electron microscopy can detect vacuolization, lesions of the membrane, and desmosomes in the first two hours after experimental induction of phytophotodermatitis.