Abstract The form of drug administration affects the success of treatment, since it can influence adherence of the patient to the therapy. The use of orodispersible films has emerged as a way to overcome some drawbacks of conventional methods of drug delivery, especially for patients experiencing difficulty in swallowing. These films are prepared using a matrix that incorporates the drug and contains other substances that confer the properties of the system. The present work describes the use of thermoplastic starch as a carrier for the model drug diclofenac, including film preparation and testing of its orodispersible potential. Preparation of the film employed a microwave oven to gelatinize and plasticize corn starch, with incorporation of the model drug, followed by solvent-casting. The samples were characterized using mechanical tests, analyses of water uptake and water content, and Fourier transform infrared spectroscopy. The results indicated that the film presented promising properties as an alternative system for oral drug administration, with good incorporation and distribution of the drug in the matrix. The material displayed satisfactory mechanical properties, which are crucial for this type of material, due to the need for oral administration and handling before use. treatment therapy delivery swallowing diclofenac potential solventcasting. solventcasting solvent casting. casting solvent-casting tests content spectroscopy

Abstract Background Cardiac arrest (CA) is a common condition associated with high mortality. The Brazilian advanced life support training TECA A (Treinamento em Emergências Cardiovasculares Avançado — Advanced Cardiovascular Emergency Training) was created to train healthcare professionals in the management of CA. However, there are no studies evaluating the effectiveness of TECA A. Objective To assess the impact of TECA A on the management of CA using a simulated CA situation. Methods Fifty-six students underwent a simulated case of CA in a manikin. The students’ performance in the management of CA was assessed for the time to first chest compression and defibrillation and for a global assessment score using a structured tool. These items were assessed and compared before and after the TECA A. Exclusion criteria were previous participation in CA trainings and absence from class. Categorical variables were compared using the McNemar test and quantitative variables using the Wilcoxon test. All tests were two-tailed, and statistical significance was set at p < 0.05. Results Compared with before TECA A, median global assessment scores were higher after TECA A (pre-training: 4.0 points [2.0-5.0] vs. 10 points [9.0-10.0]; p<0.001), the time to start chest compressions was shorter (pre-training: 25 seconds [15-34] vs. 19 seconds [16.2-23.0]; p=0.002) and so was the time to defibrillation (pre-training: 82.5 seconds [65.0-108.0] vs. 48 seconds [39.0-53.0]; p<0.001). Conclusions The TECA A promoted a higher adherence to cardiopulmonary resuscitation (CPR) guidelines and a reduction in the time elapsed from CA to first chest compression and defibrillation.

Resumo O presente artigo é resultado de uma das etapas de pesquisa acerca das questões históricas relativas ao meio ambiente de edificações de saúde em Salvador, financiada pelo CNPq, que teve a participação de bolsistas de iniciação científica e alunos de pós-graduação. Seu objetivo é estudar, através da análise das condições ambientais no Sanatório Santa Terezinha, inaugurado em 1942, em Salvador, BA, o planejamento de arquitetos modernistas para o uso da incidência solar e da ventilação cruzada como auxílio na cura da tuberculose, de forma a demonstrar que a arquitetura faria parte do processo curativo. A metodologia utilizada foi a comparação entre o modelo europeu e o utilizado no sanatório estudado, baseada em pesquisa bibliográfica sobre as características desse tipo de edificação, sendo posteriormente realizada visita, cadastro do edifício e análise de suas condicionantes climáticas com programas computacionais. Como resultado, pôde-se observar a intencionalidade dos arquitetos em proporcionar condições de incidência controlada de sol e de ventos que permitissem o conforto e o auxílio ao restabelecimento dos pacientes, de acordo com o paradigma médico da época. Na atualidade, há o retorno das preocupações ambientais e de humanização do espaço arquitetônico hospitalar. Espera-se que o planejamento climático constatado contribua como exemplo do uso da arquitetura como fator de apoio aos procedimentos de restabelecimento da saúde.

Abstract This paper is the result of one stage of a research project on historical issues related to the environment of healthcare buildings in Salvador, Brazil, funded by CNPq, with the participation of undergraduate and postgraduate scholars. Its objective is to study, through an analysis of the environmental conditions at the Sanatorium Santa Terezinha, inaugurated in 1942, in Salvador, BA, the plans made by modernist architects for the use of solar incidence and cross ventilation as an aid in healing tuberculosis, in order to demonstrate that architecture can be part of the healing process. The methodology of the investigation was to trace the history of the hospital, based on a review of the literature on the characteristics of this type of building, followed by a visit, a survey of the building and an analysis of its climate conditions using computer programs. That allowed the researchers to see evidence of the intentionality of the architects as they provide conditions of controlled incidence of sun and wind in order to enhance patients’ comfort and recovery, according to the medical paradigm of the time. In the present day, environmental concerns and the humanisation of hospitals’ architectural space are back on the agenda. The climate planning seen in this study will hopefully contribute as an example of the use of architecture as a factor to support the restoration of health.

RESUMO: Este artigo objetivou analisar a evolução da qualidade do leite cru refrigerado produzido por produtores da região da Zona da Mata, do Estado de Minas Gerais, entre os anos de 2012 e 2018. Para tal, foi utilizado o modelo linear de efeitos mistos, a fim de analisar a evolução mensal dos resultados de indicadores de composição do leite (gordura, proteína e extrato seco desengordurado - ESD), contagem de células somáticas - CCS, e contagem bacteriana total - CBT, provenientes de análises oficiais mensais de 94 produtores de leite, fornecedores de um laticínio da região. Os resultados indicaram uma redução contínua dos indicadores de composição do leite entre os anos de 2014 e 2018. Para os indicadores de CCS e CBT foram observadas apenas reduções pontuais. O fornecimento de um maior volume de leite foi associado ao aumento da CBT. A sazonalidade influenciou todos os indicadores de qualidade analisados. De posse desses resultados, pode-se afirmar que, apesar das ações implementadas pelo Programa Nacional de Melhoria de Qualidade do Leite - PNMQL, a qualidade do leite cru refrigerado, proveniente dos produtores avaliados, piorou ao longo do período em análise.

ABSTRACT: This paper aimed to analyze the evolution of the quality of raw milk produced by producers in the Zona da Mata region, in the state of Minas Gerais, between 2012 and 2018. For this purpose, we used the linear mixed-effects model to analyze the monthly evolution of the results of milk composition indicators (fat, protein, and defatted dry extract - DDE), somatic cell count - SCC, and total bacterial count - TBC, from the official monthly registry of 94 milk producers, suppliers of a region’s dairy. Results indicate a continuous reduction in the milk composition indicators between 2014 and 2018. For the SCC and TBC indicators, we identified only one-off reductions. The supply of a larger volume of milk was associated with increased TBC. Seasonality influenced all quality indicators analyzed. According to these results, we concluded that the analyzed quality of chilled raw milk offered by producers was proven worse from 2012 to 2018, despite the actions implemented by the National Milk Quality Improvement Program - PNMQL.

Resumo Objetivo: O governo brasileiro aprovou regulamentos para promover o mercado de títulos privados. Em 2009, a Instrução 476 da Comissão de Valores Mobiliários relaxou as exigências para a emissão de títulos. Em 2011, a Lei 12.431 criou os títulos de infraestrutura, que permitem a isenção de impostos para pessoas físicas. Desde então, os proventos totais mais que triplicaram. Descrevemos a evolução do mercado de títulos brasileiro, as características das emissões e os emitentes, e avaliamos essa evolução de maneira crítica. Metodologia: Análise descritiva. Dados sobre o total de emissões; características dos títulos (proventos, vencimentos, rendimentos, subscrição); características dos emitentes; mercado secundário (negociação); desempenho (taxas de inadimplência e renegociação); e problemas de colocação. Resultados: As emissões de títulos são pequenas e apresentam vencimento no curto prazo. As agências internacionais são as principais fornecedoras de classificação de risco, utilizando uma escala de classificação ajustada ao Brasil. Os títulos de renda fixa são raros. A grande maioria dos títulos regulares está atrelada à taxa de juros interbancária (DI ou CDI). Apenas dois bancos universais locais dominam a atividade de subscrição. Esses bancos e suas partes relacionadas recebem mais da metade da alocação total. Menos da metade das emissões recebeu a classificação de grau de investimento e mais da metade sequer possui classificação. A incidência de eventos de crédito ex-post, mais frequentemente na forma de renegociação dos prazos dos títulos. A inadimplência estrita também é alta. A liquidez dos títulos no mercado secundário é baixa. Contribuições: Até onde sabemos, este é o primeiro artigo que descreve a microestrutura do mercado de títulos brasileiro.

Abstract Purpose: The Brazilian government approved regulations to foster the corporate bond market. In 2009, Instruction 476 of the Comissão de Valores Mobiliários (the Brazilian capital market regulator) relaxed the requirements for issuing bonds. In 2011, Law 12,431 created infrastructure bonds, which give individuals tax exemptions. Since then, aggregate proceeds have more than tripled. We describe the Brazilian bond market and the characteristics of issues and issuers; and critically evaluate this evolution. Design/methodology/approach: Descriptive analysis. Data on aggregate issuances; bond characteristics (proceeds, maturity, yields, underwriting); issuer characteristics; secondary market (trading); performance (default and renegotiation rates); and allocation of issues. Findings: Bond issues are small and bonds present a short maturity. International agencies are the main ratings providers, using a Brazilian-adjusted rating scale. Fixed-yield bonds are rare. The vast majority of regular bonds are linked to the interbank offered interest rate (DI or CDI). Only two local universal banks dominate the underwriting activity. These banks and their related parties receive more than half of the aggregate allocation. Less than half of issues have an investment grade, and more than half are not rated at all. The incidence of ex-post credit events is most frequently in the form of renegotiations of bond terms. Strict defaults are also high. Liquidity for bonds in the secondary market is low. Originality/value: To our knowledge, this is the first article to describe the microstructure of the Brazilian bond market.

Abstract Autologous fibrin matrices derived from the Leukocyte and Platelet Rich Plasma (L-PRP) and Leukocyte and Platelet Rich Fibrin (L-PRF) techniques present great potential to act as a bioactive scaffold in regenerative medicine, contributing to the maintenance of cell viability, proliferation stimulus and differentiation. In contrast, there are few studies that characterize the bioactive potential of these fibrin scaffolds by considering the process of production. The objective of this work was to characterize the intrinsic potential of maintaining cell viability of different fibrin scaffolds containing platelets and leukocytes. In order to achieve that, blood samples from a volunteer were collected and processed to obtain fibrin clots using the suggested techniques. To characterize the potential for in vitro viability, mesenchymal stem cells from human infrapatellar fat were used. The scaffolds were cellularized (1x105 cells/scaffolds) and maintained for 5 and 10 days under culture conditions with Dulbecco's Modified Eagle Medium, without addition of fetal bovine serum, and subsequently subjected to analyses by Fourrier transform infra-red spectroscopy, circular dichroism and fluorescence microscopy. The results demonstrated distinct intrinsic potential viability between the scaffolds, and L-PRP was responsible for promoting higher levels of viability in both periods of analysis. No viable cells were identified in the fibrin matrix used as controls. These results allow us to conclude that both fibrin substrates have presented intrinsic potential for maintaining cell viability, with superior potential exhibited by L-PRP scaffold, and represent promising alternatives for use as bioactive supports in musculoskeletal regenerative medicine.

Sweetened condensed milk contains various dispersed particles, such as proteins, fats, and lactose, all of which make it highly viscous. During sweetened condensed milk production, microcrystalline lactose is added in order to control the product crystallization. The purpose of this study was to characterize the behavior of commercially sold sweetened condensed milks submitted to laser particle size analysis using two different dispersion media: water and a lactose solution. The lactose solution dispersion medium extended the length of time the sweeten condensed milk samples’ original characteristics were present during the laser diffraction particle sizing measurements that were carried out. Significant difference was observed (p < 0.05) between analysis times when water was used as a dispersing medium versus when a lactose solution was used (p > 0.05). Using a lactose solution as a dispersion medium when determining particle size in sweetened condensed milk prolongs the original characteristics of the sample substance during laser diffraction particle analysis.

Abstract: Atrophoderma of Pasini and Pierini is a skin disorder affecting dermal collagen and is clinically characterized by well-defined plaques of depressed skin. Histopathological changes are subtle, and in most cases, the diagnosis requires a comparative study with healthy skin from the same anatomical site. High frequency ultrasound is a useful imaging method for diagnosis of atrophic skin changes. A case is presented in which ultrasound can support the clinical and the histopathological diagnosis of atrophoderma of Pasini and Pierini.

Resumo Fundamento: A escolha de próteses mecânicas ou biológicas para pacientes com doença de válvula cardíaca ainda não é um consenso. Objetivo: Determinar os desfechos clínicos de próteses mecânicas e biológicas nesses pacientes. Métodos: Conduzimos uma revisão sistemática e metanálise e estudos controlados randomizados (RCTs) que compararam próteses mecânicas e biológicas em pacientes com doenças de válvulas cardíaca, e avaliamos seus resultados. A busca por RCTs foi feita nas bases de dados MEDLINE, EMBASE, LILACS, CENTRAL, SCOPUS e Web of Science (do início a novembro de 2014). As metanálises foram realizadas usando variação inversa com modelos de efeitos aleatórios. Usamos o sistema GRADE para avaliar a qualidade da evidência. Um valor menor que 0,05 foi considerado significativo. Resultados: Um total de quatro RCTs foi incluído na metanálise (1528 pacientes) com acompanhamento de 2 a 20 anos. Em três estudos, foram utilizadas próteses mecânicas e biológicas mais antigas, e em um estudo próteses contemporâneas. Não foi observada diferença de mortalidade entre os pacientes que receberam próteses mecânicas e biológicas (risco relativo, RR = 1,07; IC95% 0,99-1,15). O risco de sangramento foi significativamente mais baixo nos pacientes que receberam próteses biológicas que naqueles com próteses mecânicas (RR = 0,64; IC95% 0,52-0,78). Contudo, as reoparações foram mais frequentes em pacientes com próteses biológicas (RR = 3,60; IC95% 2,44-5,32). Não houve diferenças estatisticamente significativas entre pacientes com próteses biológicas e mecânicas em relação à embolia arterial sistêmica e endocardite infecciosa (RR = 0,93; IC95% 0,66-1,31; RR = 1,21; IC95% 0,78-1,88, respectivamente). Resultados entre os estudos com próteses modernas e antigas foram similares. Conclusões: A taxa de mortalidade e o risco de eventos tromboembólicos e endocardite foram similares entre os pacientes que receberam próteses biológicas e mecânicas. O risco de sangramentos foi aproximadamente um terço menor nos pacientes com próteses biológicas que mecânicas, ao passo que o risco de reoperação foi mais que três vezes maior nos pacientes com próteses biológicas.

Abstract Background: The choice of a mechanical (MP) or biological prosthesis (BP) for patients with valvular heart disease undergoing replacement is still not a consensus. Objective: We aimed to determine the clinical outcomes of MP or BP placement in those patients. Methods: We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) that compared biological prostheses and mechanical prostheses in patients with valvular heart diseases and assessed the outcomes. RCTs were searched in the MEDLINE, EMBASE, LILACS, CENTRAL, SCOPUS and Web of Science (from inception to November 2014) databases. Meta-analyses were performed using inverse variance with random effects models. The GRADE system was used to rate the quality of the evidence. A P-value lower than 0.05 was considered significant. Results: A total of four RCTs were included in the meta-analyses (1,528 patients) with follow up ranging from 2 to 20 years. Three used old generation mechanical and biological prostheses, and one used contemporary prostheses. No significant difference in mortality was found between BP and MP patients (risk ratio (RR = 1.07; 95% CI 0.99-1.15). The risk of bleeding was significantly lower in BP patients than MP patients (RR = 0.64; 95% CI 0.52-0.78); however, reoperations were significantly more frequent in BP patients (RR = 3.60; 95% CI 2.44-5.32). There were no statistically significant differences between BP and MP patients with respect to systemic arterial embolisms and infective endocarditis (RR = 0.93; 95% CI 0.66-1.31, RR = 1.21; CI95% 0.78-1.88, respectively). Results in the trials with modern and old prostheses were similar. Conclusions: The mortality rate and the risk of thromboembolic events and endocarditis were similar between BP and MP patients. The risk of bleeding was approximately one third lower for BP patients than for MP patients, while the risk of reoperations was more than three times higher for BP patients.

RESUMO: A água é um insumo essencial para qualquer agroindústria, utilizada para diversas finalidades, como em procedimentos de higienização, trocadores de calor e formulação de bebidas. Dessa forma, a cobrança pelo uso da água bruta, regulamentada no Brasil pela Política Nacional de Recursos Hídricos - Lei nº 9.433/97 e leis estaduais, é um tema importante para a indústria de alimentos, uma vez que pode alterar o perfil dos custos de produção de bens alimentícios. Assim, este artigo objetivou estimar os incrementos de preço provenientes da cobrança pelo uso da água bruta em uma indústria de laticínios e avaliar o potencial benefício dessa arrecadação, comparando-a com alguns investimentos previstos em planos de bacias hidrográficas. Para tal, foi utilizado um estudo de caso de uma indústria de laticínios com produção de 1 t/d de queijo mussarela, no Estado de Minas Gerais. Os resultados indicaram que o valor da cobrança pelo uso da água bruta entre as bacias hidrográficas com cobrança vigente pode variar em até 131%. O incremento de preço obtido pela cobrança de uso da água bruta foi baixo (0,04-0,09% dos custos de produção), indicando que este pode ser absorvido pelas indústrias. Por outro lado, os valores arrecadados pelas bacias podem ser decisivos para a efetivação de diversas ações voltadas para a melhoria da quantidade e da qualidade da água, beneficiando as próprias indústrias. Nos modelos de cobrança vigentes, no Estado de Minas Gerais, é possível reconhecer que a cobrança é uma iniciativa promissora para valorar economicamente a água. Porém, a cobrança ainda parece ser insuficiente para assegurar a racionalização do seu uso.

ABSTRACT: Water is an essential input for any agribusiness, used for various purposes such as hygiene procedures, heat exchangers and beverage formulation. Water charging, regulated in Brazil by the National Water Resources Policy (Federal Law 9.433/97), is an important issue for the food industry, since it may change the profile of food production costs. Thus, this article aimed to estimate the price increases in the dairy industry from the use of water charging and evaluate the potential benefit of this collection, comparing it to some planned investments in basins plans. We used a case study of a dairy that produced 1 t/d of mozzarella cheese in the state of Minas Gerais. Results indicated that water charging among the basins with current collection models may vary up to 131%. The increase in price related to water charging was low (0.04-0.09% of production costs), indicating that it can be absorbed by the industry. Conversely, values collected by the basins may be decisive for implementation of several actions aiming an average quantity and quality of water, which is good for the industry, itself. In the current charging collection models in the state of Minas Gerais, it is possible to recognize that collection is a promising initiative for the economical valuation of water. However, charging still seems to lack sufficiency in safety and a rationalization for its use.

Resumo O artigo põe em questão uma afirmação de Jung de que a ética se resumiria na relação entre homem e Deus. Tomando-a como problema, busca uma articulação entre os conceitos junguianos para uma resposta do que se entende por ética nessa perspectiva. Esboçamos um percurso que passa pelos problemas dos opostos morais, pelo confronto com a sombra e, por fim, abordamos a questão que inicia a pesquisa. Ao final, argumentamos que tal relação aduzida por Jung é, em termos psicológicos, a relação entre o eu e o si-mesmo. A ética seria nesse sistema uma resposta a uma outra voz suprarracional, “a voz de Deus”, que, para além da pura estética da imagem, conjuga consciente e inconsciente; demanda a totalidade da personalidade.

Abstract The article calls into question Jung’s assertion that ethics would be summed up in the relationship between man and God. Taking it as a problem, it seeks an articulation between the Jungian concepts for an answer of what is meant by ethics in this perspective. We outline a course that goes through the problems of moral opposites, the confrontation with the shadow, and finally, we approach the question that starts the research. In the end, we argue that such a relationship referred to by Jung is, in psychological terms, the relationship between the ego and the Self. Ethics would be in this system a response to that other supra-rational voice, “the voice of God,” which, beyond the pure aesthetics of the image, combines conscious and unconscious; demands the entire personality.

Resumen El artículo pone en cuestión una afirmación de Jung de que la ética se resumiría en la relación entre hombre y Dios. Tomándola como problema, busca una articulación entre los conceptos junguianos para una respuesta de lo que se entiende por ética en esa perspectiva. Esbozamos un recorrido que pasa por los problemas de los opuestos morales, por el enfrentamiento con la sombra y, por fin, abordamos la cuestión que inicia la investigación. Al final, argumentamos que tal relación planteada por Jung es, en términos psicológicos, la relación entre el yo y el sí mismo. La ética sería en ese sistema una respuesta a otra voz, suprarracional, “la voz de Dios”, que más allá de la pura estética de la imagen, conjuga consciente e inconsciente; demanda la totalidad de la personalidad.

Résumé L’article remet en question l’affirmation de Jung selon laquelle l’éthique serait résumée dans la relation entre l’homme et Dieu. Prenant cela comme un problème, il cherche une articulation entre les concepts jungiens pour une réponse de ce que l’on entend par éthique dans cette perspective. Nous décrivons un parcours qui passe par les problèmes d’opposés moraux, la confrontation avec l’ombre, et enfin, nous abordons la question qui lance la recherche. En fin de compte, nous affirmons qu’une relation telle que Jung est, en termes psychologiques, la relation entre le soi et le soi. L’éthique serait dans ce système une réponse à une autre voix supra-rationnelle, “la voix de Dieu”, qui, au-delà de la pure esthétique de l’image, se combine consciemment et inconsciemment; demande toute la personnalité.

ABSTRACT Objective: The objective was to evaluate the association between sarcopenia (EWGSOP) and osteoporosis in older adults. Subjects and methods: This is a cross sectional analysis of a baseline evaluation of the SARCopenia and OSteoporosis in Older Adults with Cardiovascular Diseases Study (SARCOS). Three hundred and thirty-two subjects over 65 years of age were evaluated. Sarcopenia was determined by EWGSOP flowchart and Osteoporosis was established by WHO's criteria. Physical function, comorbidities and medications were evaluated. Results: Women were older (79.8 ± 7.2 years) than men (78.21 ± 6.7 years) (p = 0.042). Osteoporosis occurred in 24.8% of men, and in 42.7% of women (p < 0.001); sarcopenia occurred in 25.5% of men and in 17.7%, of women (p = 0.103). Osteoporosis was diagnosed in 68% of sarcopenic women, however only 20.7% (p = 0.009) of women with osteoporosis had sarcopenia; in older men, 44.7% of individuals with sarcopenia presented osteoporosis and 42.9% (p = 0.013) of men with osteoporosis showed sarcopenia. In an adjusted logistic regression analyses for sarcopenia, osteoporosis presented a statistically significant association with sarcopenia in men [OR: 2.930 (95% CI: 1.044-8.237; p = 0.041)] but not in women [OR: 2.081 (0.787-5.5; p = 0.142)]; in the adjusted logistic regression analyses for osteoporosis, a statistically significant association occurred in men [OR: 2.984 (95% CI: 1.144-7.809; p = 0.025)], but not in women [OR: 2.093 (0.962-3.714; p = 0.137)]. Conclusion: According to sex, there are significant differences in the association between sarcopenia EWGSOP and osteoporosis in outpatient older adults. It is strong and significant in males; in females, despite showing a positive trend, it was not statistically significant.

Abstract Two new Brazilian species of Drosophila (subgenus Drosophila) are described and illustrated: Drosophila asymmetrica sp. nov. and Drosophila peixotoi sp. nov. Both species were collected, and emerged, from inflorescences of Goeppertia monophylla (Marantaceae) in the urban Forest Reserve of the Instituto de Biociências da Universidade de São Paulo and their types will be deposited in the Museu de Zoologia da USP. The former species, which could not be assigned to any known group, has a conspicuously asymmetric aedeagus and a narrow oviscapt valve. The latter species belongs to the guarani group and is closely related to D. guaru, D. ornatifrons and D. subbadia, from which it can be distinguished by the presence of just one conspicuous large black spine at inner lower tip of cercus instead of two spines.

RESUMO: O objetivo do presente estudo foi descrever e caracterizar, pela primeira vez, dois surtos de salmonelose causados por Salmonella Ndolo em potros e bezerros do Brasil e comparar esses isolados com Salmonella Ndolo previamente identificada em répteis assintomáticos. Os animais infectados apresentaram febre, letargia e diarreia profusa. Os isolados foram submetidos a testes de susceptibilidade a antimicrobianos e foram caracterizados conforme a presença de genes de virulência e diversidade genética, utilizando-se o ERIC-PCR. Salmonella Ndolo foi identificado em amostras fecais de dois potros e quatro bezerros. Um isolado de bezerro foi resistente a amoxicilina/ácido clavulanico, trimethoprima/sulfametoxazol e florfenicol. Estirpes de dois outros bezerros foram resistentes a oxitetraciclina. Todos os genes de virulência testados foram identificados nos isolados e dois grandes grupos de estirpes geneticamente relacionadas foram identificados pelo ERIC-PCR, um para cada surto. Esse é o primeiro relato de Salmonella Ndolo em animais domésticos e sintomáticos. Previamente, esse sorovar foi identificado apenas em infecções humanas. A presença de fatores de virulência relevantes em todos os isolados e a detecção de estirpes multirresistentes a antimicrobianos destaca a importância do monitoramento de sorovares associados a salmonelose em animais domésticos.

ABSTRACT: The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All virulence genes tested were present in the isolates, and two major clusters of closely related strains were identified by ERIC-PCR, each per outbreak. This is the first report of Salmonella Ndolo infection in domestic and symptomatic animals. Previously, this serovar had been identified only in human infections. The presence of relevant virulence genes in all Salmonella Ndolo isolates and the detection of antimicrobial multi-resistant strains highlighted the importance of monitoring serovars associated with salmonellosis in domestic animals.

Resumo Fundamentos: O diabetes mellitus é uma doença crônica grave que leva a complicações sistêmicas, como a disfunção cardiovascular. Estudos anteriores de terapia celular obtiveram resultados promissores com utilização de células estromais mesenquimais (CEM) derivadas de medula óssea de animais saudáveis (CEMc) em modelos de animais diabéticos. No entanto, a capacidade das CEM derivadas de ratos diabéticos em melhorar parâmetros cardíacos funcionais é ainda desconhecida. Objetivos: Investigar se CEM derivadas de medula óssea de ratos diabéticos (CEMd) poderiam contribuir para a recuperação metabólica e de propriedades elétricas cardíacas em outros ratos também com diabetes. Métodos: O diabetes foi induzido em ratos Wistar com estreptozotocina. As CEM foram caracterizadas por citometria de fluxo, análise morfológica e imunohistoquímica. A função elétrica cardíaca foi analisada através de registro do potencial de ação ventricular. As diferenças entre as variáveis foram consideradas significativas quando p < 0,05. Resultados: As propriedades in vitro das CEMc e CEMd foram avaliadas. Ambos os tipos celulares apresentaram morfologia, cinética de crescimento e perfil mesenquimal semelhante, e puderam ser diferenciadas em linhagens adipogênica e osteogênica. No entanto, em ensaios para unidades formadoras de colônias de fibroblastos (UFC-F), as CEMd formaram mais colônias em comparação às CEMc quando cultivadas em meio com ou sem hidrocortisona (1 µM). Para comparar o potencial terapêutico das células, os animais foram divididos em quatro grupos experimentais: não diabéticos (CTRL), diabéticos (DM), diabéticos tratados com CEMc (DM + CEMc) e diabéticos tratados com CEMd (DM + CEMd). Os grupos tratados receberam uma única injeção de CEM 4 semanas após o estabelecimento do diabetes. Ambas CEMc e CEMd controlaram a hiperglicemia e a perda de peso corporal e melhoraram o remodelamento elétrico cardíaco em ratos com diabetes. Conclusão: As CEMd e CEMc possuem propriedades in vitro e potencial terapêutico semelhante em um modelo de rato com diabetes induzido por estreptozotocina. (Arq Bras Cardiol. 2017; 109(6):579-589)

Abstract Background: Diabetes mellitus is a severe chronic disease leading to systemic complications, including cardiovascular dysfunction. Previous cell therapy studies have obtained promising results with the use bone marrow mesenchymal stromal cells derived from healthy animals (MSCc) in diabetes animal models. However, the ability of MSC derived from diabetic rats to improve functional cardiac parameters is still unknown. Objectives: To investigate whether bone-marrow-derived MSC from diabetic rats (MSCd) would contribute to recover metabolic and cardiac electrical properties in other diabetic rats. Methods: Diabetes was induced in Wistar rats with streptozotocin. MSCs were characterized by flow cytometry, morphological analysis, and immunohistochemistry. Cardiac electrical function was analyzed using recordings of ventricular action potential. Differences between variables were considered significant when p < 0.05. Results: In vitro properties of MSCc and MSCd were evaluated. Both cell types presented similar morphology, growth kinetics, and mesenchymal profile, and could differentiate into adipogenic and osteogenic lineages. However, in an assay for fibroblast colony-forming units (CFU-F), MSCd formed more colonies than MSCc when cultured in expansion medium with or without hydrocortisone (1 µM). In order to compare the therapeutic potential of the cells, the animals were divided into four experimental groups: nondiabetic (CTRL), diabetic (DM), diabetic treated with MSCc (DM + MSCc), and diabetic treated with MSCd (DM + MSCd). The treated groups received a single injection of MSC 4 weeks after the development of diabetes. MSCc and MSCd controlled hyperglycemia and body weight loss and improved cardiac electrical remodeling in diabetic rats. Conclusions: MSCd and MSCc have similar in vitro properties and therapeutic potential in a rat model of diabetes induced with streptozotocin.