Abstract Purpose: To evaluate the influence of subcutaneous injection nicotine in osseointegration process on different implant surfaces. Methods: Twenty-two male rabbits were distributed into two groups according to the subcutaneous injections: (1) nicotine 3 mg/day/kg and (2) 0.9 % NaCI 3 mL/day/kg, three times a day; subgroups were then designated-machined and anodized implants were placed in the right and left tibia bones, respectively. The animals were submitted euthanasia after periods of eight weeks to determine nicotine and cotinine levels, alkaline phosphatase and biomechanical analysis. Results: The plasmatic levels of nicotine and cotinine were 0.5 ± 0.28 ng/mL and 9.5 ± 6.51 ng/mL, respectively. The alkaline phosphatase analyses in blood levels in control group were observed 40.8 ± 11.88 UI/L and 40.75 ± 12.46 UI/L, for the surfaces machined and anodized, respectively. In the test group was observed levels 37.9 ± 4.84 UI/L, for both implant surfaces. No significant differences were observed between control and test groups and between the implant surfaces regarding alkaline phosphatase blood levels. For biomechanics, no significant differences were observed in control group between the machined (25±8.46 Ncm) or anodized (31.2 ± 6.76 Ncm) implants. However, the treatment with nicotine induced higher torque than control in both machined (38.3 ± 13.52 Ncm) and anodized (35.5 ± 14.17 Ncm) implants, with p = 0.0024 and p = 0.0121, respectively. Conclusion: Subcutaneous injection of nicotine following implant insertion didn’t have effect on osseointegration, independently from the implant surface.

RESUMO Objetivo: Determinar a prevalência e a severidade da cárie dentária, ausências dentárias e restaurações pelo índice CPOD, tão bem como a eficiência dos programas de saúde bucal de pacientes com deficiência intelectual, com idades de 11 a 38 anos, que frequentavam a Associação de Pais e Amigos das Crianças Excepcionais-APAE Passo Fundo, Sul do Brasil. Métodos: A prevalência da cárie dentária foi avaliada pelo índice CPOD em 109 pacientes. A média e o desvio-padrão foram avaliados pela ANOVA unidirecional com nível de significância de 5%. Resultados: 47 dos estudantes avaliados eram do sexo feminino, e 62 do masculino, apresentando deficiência mental moderada, síndrome de Down, paralisia cerebral e epilepsia. A média do CPO-D foi de 3.93 para as idades de 11-14 anos, 3.47 para 15-19 anos, 4.74 para 20-30 anos e 5.68 para os pacientes com 31-40 anos. Não houve diferenças significativas entre o índice de CPOD, gênero e deficiência intelectual. Conclusão: Os pacientes apresentaram higiene bucal aceitável dentro das suas limitações, sugerindo que o programa de prevenção e tratamento odontológico desenvolvido no APAE-PF/RS poderia ser utilizado como modelo de atenção à saúde para pacientes com deficiência intelectual em outras instituições e outras cidades.

ABSTRACT Objective: This study was conducted to determine the prevalence and severity of dental caries, missing and restored teeth (DMFT) and the efficiency of oral health programs for patients with intellectual disabilities, from age 11 to 38, who attended at the Association of Exceptional Children’s Parents and Friends (APAE) in Passo Fundo, southern Brazil. Methods: The prevalence of dental caries was assessed by DMFT in 109 patients. The mean and standard deviation were evaluated by One-way ANOVA with 5% significance level. Results: 47 of the evaluated students were female and 62 male, with moderate mental disability, Down syndrome, cerebral paralysis and epilepsy. The DMFT average was 3.93 for 11-14 years, 3.47 for 15-19 years, 4.74 for 20-30 years and 5.68 for the group 31-40 years. There were no significant differences among the DMFT index, gender and intellectual disability for the groups. Conclusion: Patients had acceptable oral hygiene within their limitations, suggesting that prevention and dental care program developed in APAE-PF/RS could be used as a model for health care for patients with intellectual disabilities in other institutions and other cities.

RESUMO Objetivo Avaliar por meio de densitometria óssea o comportamento de dois fatores que podem inteferir no processo da osseointegração: a nicotina e a texturização superficial dos implantes. Métodos Dezenove coelhos da raça Nova Zelândia (Oryctolagus cuniculus) foram distribuídos aleatoriamente em dois grupos (teste e controle), nos quais foram colocados 38 implantes. Na tíbia direita dos animais foram inseridos dezenove implantes usinados (lisos) de 3,75 mm de diâmetro por 6,0 mm de comprimento e, na tíbia esquerda, dezenove implantes anodizados (Vulcano(r)) nas mesmas dimensões. Foram administradas no grupo teste injeções subcutâneas de nicotina 3ml/dia/kg, três vezes ao dia, o grupo controle recebeu igualmente, três vezes ao dia, solução subcutânea de NaCl (3ml/dia/kg), por oito semanas. Após esse período, procedeu-se a análise densitométrica. Resultados Considerando a densidade óptica (pixels), não houve diferenças estatisticamente significativas entre os tipos de implantes (p = 0.135) bem como entre os grupos (p=0.590). Conclusão A nicotina dentro das condições experimentais empregadas neste estudo e a texturização superficial dos implantes não interferiram na densidade óssea.

ABSTRACT Objective The objective of this experimental study was to evaluate, through bone densitometry, the performance of two factors that may influence the osseointegration process: nicotine and superficial texturization of the implants. Methods Nineteen New Zealand rabbits (Oryctolagus cuniculus) were randomly distributed in two groups (test and control), in which 38 implants were placed. 19 machined implants (flat) 3.75 mm of diameter by 6.0 mm length were placed at the right tibia of the animals and, at the left tibia, 19 anodized implants (Vulcano(r)) with the same dimensions. Subcutaneous injections of nicotine 3ml/day/kg, three times a day were administered in group, group control also received, three times a day, subcutaneous solution of NaCl (3ml/day/kg), for 8 weeks. After this period, the densitometry analysis was performed. Results Considering the optical density (pixels), there were no statistical significant differences between the types of implants (p = 0.135) as well as between groups (p=0.590). Conclusion The nicotine inside the experimental conditions used in this study and the superficial texturization of the implants indicated that they were not influencing bone density.

O propósito deste estudo foi avaliar o potencial proliferativo celular das lesões de líquen plano bucal (LPB) de pacientes sem vírus da hepatite C (VHC) por meio do método AgNOR, comparando-o ao potencial proliferativo celular da mucosa bucal normal de portadores de VHC, tratados ou não com interferon e ribavirina. Um estudo transversal foi realizado para investigar 4 grupos: 10 pacientes VHC+ sem sinais clínicos de LPB que nunca haviam sido tratados para a infecção por VHC - Grupo 1; 10 pacientes VHC+ que estavam sob tratamento com interferon e ribavirina - Grupo 2; 15 pacientes com LPB reticular histopatologicamente confirmado, sem VHC - Grupo 3; e 15 doadores de sangue sem infecção por VHC e sem sinais clínicos de LPB (Grupo 4 - Grupo de Controle). O material celular de todos os grupos foi coletado pela técnica da citologia em base líquida. Então, o material sedimentado de cada paciente foi submetido ao método da impregnação das regiões organizadoras nucleolares pela prata (AgNOR). A contagem das NORs foi realizada em 100 núcleos celulares epiteliais por paciente por meio do programa Image Tool(r). O teste Tukey HSD foi utilizado para comparar o valor médio de NORs entre os grupos e mostrou que a mucosa bucal dos pacientes VHC+ previamente tratados com fármacos anti-VHC (Grupo 2) apresentou maior número médio de NORs por núcleo em relação aos outros (p<0,05). O tratamento anti-VHC pode estar relacionado ao aumento da atividade proliferativa celular da mucosa bucal, aventando uma possível relação entre LPB e pacientes VHC+ tratados com interferon e ribavirina.

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.