ABSTRACT Background: The objective of this study was to evaluate the quality indicators for wood and charcoal from the Eucalyptus urophylla x Eucalyptus grandis hybrid (Clone GG 100) in different planting spacings. The study was conducted with the hybrid using the spacings of 2×1, 2×2, 3×2, and 3×3 m. The chemical properties of wood and charcoal were analyzed, and dry wood mass and energy per hectare were estimated. The data were subjected to the t test for comparison of means. Moreover, principal component analysis was conducted to assess the relationships between wood and charcoal indicators as a function of spacing. Results: The 2×2 m spacing showed the highest values for dry wood mass, lignin mass, mass of charcoal, and energy. The wider spacings of 3×2 and 3×3 m resulted in wood and charcoal of increased quality by showing wood of greater resistance to thermal degradation and charcoal of higher yield. Conclusion: Spacing between trees influenced wood quality indicators. The 2×2 m spacing showed higher estimates of mass and energy per hectare. However, wide spacings with an area greater than 6 m2 are more suitable for charcoal production due to their superior wood properties, especially basic density and total lignin, which influence gravimetric yields and bulk density.

ABSTRACT Background: The objective of this study is to characterize the diversity and genetic structure of A. colubrina in three Atlantic Forest fragments in the south of Espírito Santo state, using Inter Simple Sequence Repeats (ISSR) molecular markers. Genomic DNA from 85 trees was analyzed using 12 ISSR primers, generating 147 fragments, of which 109 were polymorphic (74%). Markers were characterized as moderately informative, with a mean polymorphic informational content of 0.34. Results: Low genetic diversity was found for the three fragments and in the total sample, based on Nei’s diversity parameters (H* = 0.26) and Shannon’s index (I* = 0.38). In the dissimilarity analysis, four groups were observed in the dendrogram with an exclusive clustering trend by location. The analysis of molecular variance confirmed that most of the genetic variation is found within populations (73.50%), however, with high genetic differentiation between them (Φ ST = 0.26). This result was supported by the Bayesian approach that indicated genetically structured populations. Conclusion: The data obtained reveal that forest fragmentation affected the diversity and genetic structure of A. colubrina and allow expanding knowledge for the development of effective strategies for the conservation and management of the species.

Abstract Introduction: Understanding the hydrological balance and canopy structure in forest ecology and reforestation can predict the productivity of plantations. Objectives: To monitor an incident rainfall partitioning in a plantation of Schizolobium parahyba (Vell.) S. F. Blake and Samanea saman (Jacq.) Merr. and its relationship with canopy openness and leaf area index. Materials and methods: Incident rainfall partitioning was monitored for one year in a plantation of S. parahyba (4.4-5.4 years) and S. saman (5.8-6.8 years) in Rionegro, Santander, Colombia. Each plantation had linear rain gauges installed under the canopy and around the stem of the trees selected. Results and discussion: Throughfall (Tf), canopy interception losses (I) and stemflow (Sf) in the 12 months corresponded to 77.5, 22.3 and 0.44 %, respectively, in relation to open precipitation for S. parahyba (2 270 mm) and to 84.7, 15.3 and 0.05 %, respectively, for S. saman (2 140 mm). For both species, Ip and effective precipitation were higher (P < 0.05) in the two dry periods of the year, while I and Sf were higher in the two rainy periods. Canopy openness was correlated only with I and Tf in S. saman, while leaf area index was not correlated with any variable. Conclusion: Rainfall partitioning points out different paths in the same studied environment. It is important to analyze the hydrological processes in reforestation environments by taking into account the morphology of the species involved.

Resumen Introducción: En la ecología forestal y reforestación, la comprensión del balance hidrológico y estructura del dosel puede ayudar a predecir la productividad de las plantaciones. Objetivos: Monitorear la redistribución de la precipitación incidente en una plantación de Schizolobium parahyba (Vell.) S. F. Blake y Samanea saman (Jacq.) Merril y su relación con la apertura del dosel e índice de área foliar. Materiales y métodos: La redistribución de la precipitación incidente se monitoreó durante un año en una plantación de S. parahyba (4.4-5.4 años) y S. saman (5.8-6.8 años) en Rionegro, Santander, Colombia. En cada plantación se instalaron pluviómetros lineales debajo de las copas y alrededor del fuste de los árboles seleccionados. Resultados y Discusión: La precipitación interna (Pi), pérdidas por interceptación del dosel (I) y escurrimiento fustal (Ef) en los 12 meses correspondieron al 77.5, 22.3 y 0.44 %, respectivamente, en relación con la precipitación incidente para S. parahyba (2 270 mm) y al 84.7, 15.3 y 0.05 %, respectivamente, para S. saman (2 140 mm). Para ambas especies, la Pi y la precipitación efectiva fue mayor (P < 0.05) en los dos periodos secos del año, mientras que la I y Ef fueron mayores en los dos periodos lluviosos. La apertura del dosel se correlacionó únicamente con la I y Pi en S. saman, mientras que el índice de área foliar no tuvo correlación con alguna variable. Conclusión: La distribución de la lluvia señala caminos diferentes en el mismo ambiente estudiado. Es importante investigar los procesos hidrológicos en los ambientes de reforestación considerando la morfología de las especies involucradas.

Resumo Para explorar os atributos físicos e químicos do solo e espacializar o estoque de carbono orgânico do solo em um trecho de Floresta Atlântica (Estacional Semidecidual Submontanal, Espírito Santo, Brasil), este estudo avaliou quatro camadas de solo (0-5, 5-10, 10-20 e 20-40 cm), as inter-relações dos atributos físicos e químicos e a espacialização do estoque de carbono orgânico do solo aplicando a interpolação por “cokrigagem”. Foram coletadas 12 amostras em cada camada de solo, sendo determinados os teores de nutrientes, a densidade do solo, a textura e o teor de carbono orgânico, sendo também determinado o estoque de carbono. Os atributos do solo mais significativos na variação dos dados foram verificados pela ordenação por Análise de Componentes Principais (ACP) agrupando as parcelas semelhantes quanto às características físicas e químicas. Com base na autocorrelação espacial dos atributos do solo, os dados do modelo de elevação digital (altitude e declividade) para o local estudado foram combinados para explorar a relação de coordenadas entre os parâmetros do terreno e o estoque de carbono orgânico do solo. Foi verificado o aumento da fertilidade do solo paralelo ao aumento do teor de matéria orgânica, auxiliando no entendimento das diferenças e similaridades dos sítios identificados no campo. Observa-se diferença espacial no estoque de carbono orgânico do solo, sendo o maior verificado nas áreas de menor declividade e altitude.

Abstract This study aimed to explore the physical and chemical attributes of the soil and to spatialize the soil organic carbon stock in a stretch of Atlantic Forest by evaluating four soil layers and the interrelationships of physical and chemical attributes as well as the spatialization of soil organic carbon stock by applying cokriging interpolation. Thus, 12 soil samples were collected in a soil layer, and the nutrient content, soil density, texture, soil organic carbon content and the soil carbon stock were determined. A Principal Component Analysis (PCA) grouped the most similar plots in terms of physical and chemical characteristics. Based on the spatial autocorrelation of the soil attributes, the digital elevation model data (altitude and slope) for the studied site were combined to explore the coordinate relationship between the terrain parameters and the soil organic carbon stock. We verified an increase in the soil fertility parallel to the increase in the organic matter content in the soil, helping to understand the differences and similarities of the identified sites in the field. There is a spatial difference in the soil organic carbon stock, with the largest being observed in areas of lower slope and altitude. The physical and chemical attributes of the soil in the Atlantic Forest varied according to the sampled points, being strongly related to the relief. The spatial variability altered soil organic carbon stocks in the forest under study.

Abstract The aim of the study was to investigate Pseudobombax aff. petropolitanum (PP) contribution on annual fine litterfall, carbon content, and nutrient concentration, compared to other woody species (OS) on a neotropical inselberg in Espírito Santo state, Brazil. Annual fine litterfall was systematic monthly collected (November 2011-October 2012) by means of 15 littertraps (0.25 m2) placed in five transects, oven-dried (65 °C, 72 h), weighed, and C content and nutrients concentration (N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, Zn) were estimated. PP performed lower litterfall, C content, and nutrient concentration excepting higher K concentration, compared to OS. The results suggested that PP performed higher nutrient conservation, which indicated its potential in restoring degraded areas observed in the inselberg.

Resumo A dinâmica da serapilheira é um dos processos fundamentais para o crescimento e manutenção dos fragmentos florestais nativos, sendo considerada a principal via de ciclagem de nutrientes nas florestas. Portanto, estudos sobre a serapilheira acumulada e conteúdo de nutrientes subsidiam informações para uma melhor compreensão da dinâmica dos nutrientes. O objetivo do estudo foi avaliar o estoque de serapilheira e nutrientes em diferentes estações do ano e a taxa instantânea de decomposição em um fragmento de Floresta Atlântica ao longo de dois anos. A amostragem da serapilheira foi realizada em 12 parcelas permanentes com dimensões de 20 m x 50 m. Determinou-se a massa seca da serapilheira e o teor dos nutrientes. O acúmulo médio anual de serapilheira foi de 5.269 kg ha-1. Não houve diferença estatística na quantidade de serapilheira entre as estações do ano. Houve diferença estatística nos teores de N, P, K Ca e Mg entre as diferentes estações do ano. Os estoques de nutrientes foram de 123.3, 92.8, 13.2, 11.8, 9.6 e 3.0 kg ha-1 para Ca, N, Mg, S, K e P, respectivamente. O total de nutrientes na serapilheira acumulada foi de 253,7 kg ha-1 ano-1. O tempo de renovação da serapilheira foi de 281 dias. O tempo para a decomposição de 50% e 95% da mesma foi de 196 e 850 dias, respectivamente. A produção média de serapilheira, o estoque de nutrientes e a decomposição estão de acordo com outros estudos realizados, indicando que o fragmento de Floresta Atlântica sazonal semidecidual apresenta indicadores de ciclagem de nutrientes. Esses resultados encontrados mostram que o ecossistema se encontra sustentável sob ponto de vista da ciclagem e liberação dos nutrientes.

Abstract Litter dynamics is one of the fundamental processes for the growth and maintenance of native forest fragments, being considered the main pathway for nutrient cycling in forests. Therefore, studies on accumulated litter and nutrient content provide information for a better understanding of nutrient dynamics. The aim of the study was to evaluate leaf litter and nutrient stock in different seasons and the instantaneous rate of decomposition in an Atlantic Forest Fragment over two years. Litter sampling was carried out in 12 permanent plots with dimensions of 20 m x 50 m. Litter dry mass and nutrient concentration were determined. The average annual accumulation of litter was 5269 kg ha-1. There was no statistically significant difference in the amount of litter between seasons. There was a statistically significant difference in the contents of N, P, K Ca and Mg between the different seasons. Nutrient stocks were 123.3, 92.8, 13.2, 11.8, 9.6, 3.0 kg ha-1 year-1 for Ca, N, Mg, S, K and P respectively. The total of nutrients in the accumulated litter was 253.7 kg ha-1 year-1. The litter renewal time was 281 days. The times required for 50% and 95% litter decomposition were 196 and 850 days. The average litter stocks, nutrients and decomposition are in line with other studies, indicating that the Atlantic Forest fragment seasonal semi-deciduous presents indicators of nutrient cycling. These results show that the ecosystem is sustainable from the point of view of cycling and nutrient release.

ABSTRACT Background: Background: Eucalypt harvest residues are critical to nutrient balances and forest soil productivity mainly in areas with consecutive forest rotations. However, nutrient release and their relations over decomposition are still poorly understood in tropical sites. We aimed to understand how nutrients are released from leaves, branches, and bark (as harvest residues), and how nutrient concentrations could be limiting the decomposition of each fraction. We measured mass loss and nutrient release of Eucalyptus urophylla x Eucalyptus grandis harvest residues for 365 days in Southeast Brazil. Results: Results: Leaves fraction showed a faster decomposition rate than branches and bark, but nutrient accumulation was observed for all harvest residues, especially bark and branches at later stages of the decomposition. Decomposition of all harvest residues seemed to be P-limited with a stronger limitation occurring for leaf litter decomposers. The decomposition of more lignified residues such as bark and branches was less influenced by climatic variables than leaf decomposition. Conclusion: Conclusion: Eucalypt harvest residues had a great contribution to soil fertility, and retaining them in the soil systems will enhance soil fertility in the short (leaves) and long-term (branches and bark) and can partially supply the nutrients for the next rotation.

ABSTRACT Background: The objective of this study was the genetic characterization of remaining populations of the species Paratecoma peroba occurring in fragments of the Atlantic Forest, by estimating parameters of diversity and genetic structure. The study was carried out in two forest fragments, the Atlantic Forest Environmental Education Pole (area 1) and the Pacotuba National Forest (area 2), where 93 adult trees were identified. Results: Ten Inter Simple Sequence Repeats (ISSR) primers were used in genotyping, obtaining 112 amplified bands with 87.5% polymorphism. The genetic diversity estimated for the populations from the Nei (H*) and Shannon (I*) indices was higher for area 1. For the joint data, moderate genetic diversity was observed, referring to the average values of 0.26 and 0.40, obtained for the H* and I* indices, respectively. Molecular variance analysis resulted in moderate differentiation between populations (Φ ST = 0.143), while gene flow analysis (N m = 6.69) revealed the occurrence of similar alleles between them. However, the predominance of a single genetic group in area 2, revealed from Bayesian approach, indicates that its genetic structure, possibly generated by the current scenario of fragmentation of the Atlantic Forest and the distancing of populations, affecting the contemporary gene flow. Conclusion: Despite the moderate genetic diversity of the species for the area 2 population, actions toward inclusion of seedlings obtained from seeds from neighboring fragments, including area 1, and the increase in the connectivity of forest fragments through ecological corridors, could assist in augmenting its genetic variation.

Resumo A dinâmica de serapilheira é um dos processos fundamentais para o crescimento e manutenção de fragmentos florestais nativos, sendo considerada a principal via da ciclagem de nutrientes em florestas. Dessa forma, estudos sobre produção de serapilheira e conteúdo de nutrientes fornecem subsídios que proporcionam um melhor entendimento da dinâmica dos nutrientes. O objetivo do estudo foi identificar a sazonalidade e as condições meteorológicas que influenciam na quantifidade e no retorno de nutrientes através da serapilheira em um fragmento de Floresta Atlântica. A amostragem de serapilheira foi realizada mensalmente em 12 parcelas permanentes. Cada parcela continha 5 coletores distribuídos sistematicamente. A serapilheira foi classificada, quantificada a massa seca e nutrientes nas frações folhas e galhos + miscelânea. Foi constatado comportamento sazonal com as maiores deposições na estação do inverno. A produção média anual foi de 6,78 Mg ha-1, sendo 64,9% composta por folhas. A entrada média de nutrientes anual foi de 135.1, 115.7, 39.7, 23.5, 17.6 e 4.6 kg ha-1 para Ca, N, K, Mg, S e P respectivamente. A variável meteorológica precipitação apresentou influencia no padrão de deposição. O aumento na eficiência do uso de nutrientes no segundo ano em comparação com o primeiro indica que as plantas estrategicamente podem estar retranslocando quantidades relativas de seus nutrientes sob condições de estresse hídrico.

Abstract Litter dynamics is one of the fundamental processes for the growth and maintenance of native forest fragments, being considered the main pathway for nutrient cycling in forests. Studies on litter production and nutrient content therefore provide insights that provide a better understanding of nutrient dynamics. This study identifies the seasonality and meteorological conditions that influence the quantity and return of nutrients through litter in an Atlantic Forest fragment. Litter sampling was carried out monthly in 12 permanent plots. Each plot contained 5 littertraps distributed systematically. The litter was classified, and the dry mass and nutrients in the leaves and branches and miscellaneous fractions was quantified. Seasonal behavior was observed, with the highest depositions in the winter season. The average annual production was 6.78 Mg ha-1, with 64.9% being composed of leaves. The mean annual nutrient intake was 135.1, 115.7, 39.7, 23.5, 17.6 and 4.6 kg ha-1 for Ca, N, K, Mg, S and P, respectively. The meteorological variable precipitation influenced the deposition pattern. The increase in nutrient-use efficiency in the second year compared to the first indicates that plants strategically may be re-translocating relative amounts of their nutrients under water stress conditions.

Resumo O objetivo deste trabalho foi estabelecer um protocolo eficiente para a organogênese in vitro de Dalbergia nigra. Para isso, plântulas com 30 dias de idade foram seccionadas em seus nós cotiledonares e segmentos nodais. Esses materiais foram cultivados em meio com diferentes combinações das citocininas 6-benzilaminopurina e tidiazuron. Após 60 dias em sala de crescimento mantida a 27°C e submetida a fotoperíodo de 16 horas, foram analisadas as características de crescimento. Explantes dos nós cotiledonares apresentam maior potencial morfogenético, independentemente da adição de citocininas. Nós cotiledonares em meio suplementado com 2.22 µmol L-1 de BAP são a melhor combinação para a propagação in vitro de D. nigra.

Abstract The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra.

Resumo Constitui objetivo deste artigo investigar a convergência das diretrizes de integridade, compliance e anticorrupção introduzidas pela Lei das Estatais, em face das melhores práticas internacionais. Adotam-se, como parâmetro de análise, as Diretrizes em Anticorrupção e Integridade para Empresas de Propriedade Estatal, publicadas pela Organização para a Cooperação e Desenvolvimento Econômico (OCDE). De natureza descritiva, o estudo articula pesquisa bibliográfica e documental e, para a consecução dos objetivos propostos, a técnica adotada envolve a análise comparativa entre o sistema de integridade, compliance e anticorrupção introduzido pela Lei das Estatais e as diretrizes estabelecidas pela OCDE. Como resultado, observou-se que a Lei das Estatais apresenta um grau de convergência considerável em vista das práticas analisadas, tendo introduzido no ordenamento jurídico brasileiro um arranjo normativo de integridade, compliance e anticorrupção que caminha em direção ao que se poderia considerar o ‘estado da arte’. No entanto, a despeito de substantivamente aderente às recomendações transnacionais, a lei ainda apresenta limitações e omissões em pelo menos três perspectivas: gestão integrada de riscos; controles internos, ética e compliance; e autonomia das estatais e de seus órgãos decisores. Os resultados da pesquisa sinalizam para uma agenda propositiva de estudos futuros voltados à investigação das alternativas de aperfeiçoamento da Lei das Estatais e do ambiente institucional brasileiro. Também sinaliza para uma agenda de estudos aplicados, devotada a investigar e compreender os desafios da implementação da lei e seus resultados concretos no universo das companhias estatais.

Abstract This article aims to investigate the convergence of the integrity, compliance, and anti-corruption guidelines introduced by the Brazilian law of state-owned companies, in view of the best international practices, adopting as an analysis parameter the Anti-Corruption and Integrity Guidelines for State-Owned Companies, published by the Organisation for Economic Co-operation and Development (OECD). This descriptive study used bibliographic and documentary research and adopted a comparative analysis between the integrity, compliance, and anti-corruption system introduced by the Brazilian law of state-owned companies and the guidelines established by the OECD. The practices analyzed suggest a considerable degree of convergence between the Brazilian legislation and the OEDC guidelines. Thus, the Brazilian legal system presents what can be considered a cutting-edge normative arrangement of integrity, compliance, and anti-corruption practices. However, despite substantially adhering to the transnational recommendations, the law still presents gaps and omissions in at least three perspectives: integrated risk management; internal controls, ethics, and compliance; and autonomy of state-owned companies and their decision-making bodies. The research results point to a propositional agenda for future studies, focusing on investigating the alternatives for improving the law of state-owned companies and the Brazilian institutional environment, based on the gaps identified. It also points to an agenda of applied studies devoted to investigating and understanding the challenges of law implementation and its concrete results in the universe of state-owned companies.

Resumen El objetivo de este artículo es investigar la convergencia de las directrices de integridad, cumplimiento y anticorrupción introducidas por la ley de responsabilidad de las empresas estatales, en vista de las mejores prácticas internacionales, adoptando como parámetro de análisis las Directrices Anticorrupción e Integridad para Empresas del Estado, publicadas por la Organización para la Cooperación y el Desarrollo Económicos (OCDE). De naturaleza descriptiva, el estudio articula la investigación bibliográfica y documental. Para lograr los objetivos propuestos, la técnica adoptada implica un análisis comparativo entre el sistema de integridad, cumplimiento y anticorrupción introducido por la ley de responsabilidad de las empresas estatales y las pautas establecidas por la OCDE. Como resultado, se observó que la ley de responsabilidad de las empresas estatales presenta un grado considerable de convergencia en vista de las buenas prácticas analizadas, al haber introducido en el sistema legal brasileño un arreglo normativo de integridad, cumplimiento y anticorrupción, que avanza hacia lo que podría considerarse el último avance. Por otro lado, a pesar de cumplir sustancialmente con las recomendaciones transnacionales, la ley aún presenta brechas y omisiones en al menos tres perspectivas: gestión integrada de riesgos; controles internos, ética y cumplimiento; y autonomía de las empresas estatales y sus órganos de decisión. Los resultados de la investigación apuntan a una agenda propositiva para futuros estudios, con un enfoque en la investigación de alternativas para mejorar la ley de responsabilidad de las empresas estatales y el entorno institucional brasileño. Por otro lado, también apunta a una agenda de estudios aplicados dedicada a investigar y comprender los desafíos de la implementación de la ley y sus resultados concretos en el universo de las empresas estatales.

Abstract The objective of this study was to analyse the initial medium-term growth of a P. reticulata settlement under different phosphorus doses. The experiment was carried out in an area of Atlantic Forest, Sooretama, Northern Espírito Santo State, Brazil. Five doses of P per each tree (0 g, 27 g, 47 g, 67 g and 87 g) were tested in a randomized block design with three replicates and 18 plants per plot. At 3 months, 9 months and 15 months, height and diameter were measured at the soil level. At 36 months height, diameter at breast height, survival, basal area, and nutritional evaluation of the trees were measured. A logistic model was also used to model the growth in height of individual trees and a logistic model considering fixed effects and mixed form (fixed and random effects), in order to verify if the inclusion of the random effect (tree) would result in gains in terms of accuracy in the estimation of the future height of P. reticulata trees. The species presents potential for use in reforestation programs under edaphoclimatic conditions tested and under similar conditions. The species was not very demanding in P, and the addition of this nutrient promotes negative effects on its growth in the short and medium term. Regarding the model of growth in height, the logistic model adjusted in the mixed form showed to be superior to the traditional adjustment.

Resumen El objetivo de este estudio fue analizar el crecimiento inicial a mediano plazo de un asentamiento de P. reticulata bajo diferentes dosis de fósforo. El experimento se llevó a cabo en un área del Bosque Atlántico, Sooretama, norte del estado de Espírito Santo, Brasil. Se probaron cinco dosis de P por árbol (0 g, 27 g, 47 g, 67 g y 87 g) en un diseño de bloques al azar con tres repeticiones y 18 plantas por parcela. A los 3 meses, 9 meses y 15 meses se midió la altura y el diámetro a nivel del suelo. A los 36 meses de la siembra, se midió el diámetro a la altura del pecho, la supervivencia y el área basal y se hizo una evaluación nutricional de los árboles. También se utilizó un modelo logístico para modelar el crecimiento en altura de árboles individuales y el modelo logístico considerando efectos fijos y forma mixta (efectos fijos y aleatorios) para verificar si la inclusión del efecto aleatorio (árbol) resultaría en ganancias en términos de precisión en la estimación de la altura futura de los árboles de P. reticulata. La especie presenta potencial para su uso en programas de reforestación en condiciones edafoclimáticas probadas y en condiciones similares. La especie no fue muy exigente en fósforo y la adición de este nutriente promovió efectos negativos en su crecimiento a corto y mediano plazo. Con respecto al modelo de crecimiento en altura, el modelo logístico ajustado en forma mixta mostró ser superior al ajuste tradicional.

ABSTRACT: The evaluation of diversity and genetic structure allows us to verify with precision the effect of evolutionary and anthropic processes on species. The objective of this research was to evaluate the divergence and the genetic structure of two natural populations of Dalbergia nigra, using molecular markers Inter Simple Sequence Repeats (ISSR). Leaf samples were collected from two populations, located in the National Forest of Pacotuba and the Private Natural Heritage Reserve of Cafundó. Eight ISSR primers were used, which resulted in 97 bands, with 68.04% of polymorphism. Based on the joint data, the values of 0.33 for the Nei index (H*) and 0.50 for the Shannon index (I*) indicated moderate to high genetic diversity, being influenced by the presence of genetically dissimilar individuals in the National Forest of Pacotuba. Most of the genetic divergence was intrapopulational (85.96%), with moderate differentiation between populations (ΦST = 0.1404). The estimated historical gene flow between the fragments was low (Nm = 3.21) when compared with results from other tree species, and the genetic structuring analysis separated the populations into two groups, corresponding to the two populations evaluated. The results indicate a small genetic share among populations, however, populations are becoming structured. The satisfactory levels of genetic diversity benefit the use of the trees as matrixes for programs of restoration and recovery of degraded areas, connectivity of landscapes, and sustainable use of forest resources.

ABSTRACT The usual propagation method for Dalbergia nigra is via seedings; however, the seasonal supply has a variable quantity of seeds which limits the continuous production of seedlings. Therefore, the objective of this study was to establish a vegetative propagation protocol via mini-cuttings technique for D. nigra from juvenile shoots obtained from seedlings. The experimental design was completely randomized in a 2 x 2 x 4 factorial scheme, with two geographical origins: two controlled release fertilizer doses (4.0 and 8.0 g.dm-³) and four indole-3-butyric acid (IBA) concentrations (0; 2,000; 4,000; and 6,000 mg.Kg-1), with four replications. The mini-cuttings were removed from the tubes after 60 days of propagation in the greenhouse, and the substrate was completely removed by washing in running water to observe the roots. The survival percentage, rooting, rooting speed and variables related to mini-cuttings vigor (largest root length (RL, cm.plant-1), number of roots (NR), surface area (SA, mm2), root volume (RV, mm³) and root dry mass (RDM, g.plant-1) were measured. The results indicated that D. nigra has an aptitude for vegetative propagation by mini-cuttings with survival values and rooting greater than 70%. Geographical origins present a different set of responses for survival, rooting and vigor of mini-cuttings. Stock plant nutrition influences mini-cuttings root growth. IBA concentrations did not promote an effect on the mini-cuttings rooting percentage; however, exogenous auxin at the dose of 6,000 mg.Kg-1 provided mini-cuttings with greater vigor in their radical system. Nonetheless, further research is suggested to define the feasibility of using this regulator in the vegetative propagation of this species.

ABSTRACT Paratecoma peroba is a native forest species of great economic and ecological interest, currently at risk of extinction. In addition to the difficulty of finding adult individuals of this species, plant production is limited, so the use of mini-cuttings can facilitate the spread of P. peroba. Thus, the objective of this study was to analyse the effect of different doses of controlled release fertilizer (CRF) on rooting, growth, photosynthetic metabolism and nutritional balance of P. peroba clones propagated by mini-cuttings. Five doses of CRF (0, 2, 4, 6 and 8 kg.m-3) were tested, arranged in a completely randomized design with 12 repetitions and each repetition consisted of eight clones. Evaluations were carried out in the three stages of clones production: 1) Survival at 60 days in a greenhouse, 2) Rooting at 105 days in a shade house; and 3) Evaluation Productivity index, morphological variables, physiological metabolism, and nutritional through macronutrient contents, diagnosis and recommendation integrated system (DRIS) indices and the average nutritional balance index of clones at 120 days in the area of hardening. There was no influence of CRF on survival and rooting of mini-cuttings. The CRF favoured a higher productivity index, vegetative growth and physiological activity of the clones. The 6 kg.m-3 dose was sufficient to obtain nutritionally balanced clones.