Abstract Annona leptopetala (R.E.Fr.) H. Rainer, Annonaceae, is used in folk medicine like antitumor and anti-inflammatory. The aim of this study was to determine chemical composition, toxicity and antitumor activity of A. leptopetala leaves volatile oil. Fresh leaves were hydrodistilled and then the volatile oil chemical composition was assessed by gas chromatography and mass spectrometry. Toxicity was assessed using haemolysis, micronucleus and acute toxicity protocols. Antitumor effects were determined in vitro and in vivo, using sulforhodamine B assay and sarcoma 180 murine tumor model, respectively. Spathulenol was the major component identified (12.56%). The volatile oil showed in vitro antitumor activity mainly in leukemia cell line (K-562), with Total growth inhibit (TGI) (concentration producing TGI) of 0.64 µg/ml. In other hand, the volatile oil <250 µg/ml did not inhibit HaCat non-tumor cell line growth. The concentration that produced 50% haemolysis was 372.8 µg/ml. The 50% lethal dose in mice was approximately 447.2 mg/kg intraperitoneally. Sarcoma 180 tumor growth inhibition rates were 59.29% and 58.77% at 100 and 150 mg/kg intraperitoneally, respectively. The volatile oil presented moderate gastrointestinal toxicity and no genotoxicity was observed at 350 mg/kg. Thus, the volatile oil shows antitumor activity with moderate toxicity.

O objetivo deste estudo foi descrever o perfil antropométrico dos idosos residentes em Campina Grande - PB. Este estudo é populacional, transversal e domiciliar. Os dados são apresentados sob a forma de média, desvio-padrão e percentil (P5, P10, P25, P50, P75, P90 e P95) do peso, estatura, índice de massa corporal (IMC), perímetro do braço (PB), dobra cutânea tricipital (DCT), circunferência muscular do braço (CMB), perímetro da panturrilha (PP) e perímetro da cintura (PC). Foi realizado o teste t Student para verificar a diferença dos valores médios das variáveis entre os sexos. Foi realizada a análise de variância (ANOVA) para verificar o efeito da idade nas variáveis. Foram avaliados 806 idosos (60 anos ou mais). Os valores médios de todas as variáveis apresentaram diferença estatisticamente significativa entre os sexos. As médias das variáveis peso, estatura, CMB, PP e PC foram significativamente maiores entre os homens, enquanto que as médias de IMC, PB e DCT foram significativamente maiores entre as mulheres. Todas as variáveis apresentaram tendência de declínio entre os grupos etários mais avançados. Os resultados mostram que o padrão antropométrico dos idosos deste estudo segue a tendência de outros estudos, no entanto, os valores diferem. Dessa forma, sugere-se que estes possam ser usados para auxiliar na avaliação antropométrica de idosos.

This study aimed to describe the anthropometric information on elderly people in Campina Grande, PB, Brazil. It is a populational, cross-sectional household-based study. Data were presented as means, standard deviations and percentiles (5th, 10th, 25th, 50th, 75th, 90th and 95th) for weight, stature, body mass index (BMI), mid upper arm circumference (MAC), triceps skinfold thickness (TST), arm muscle circumference (AMC), calf circumference (CC) and waist circumference (WC). The analyses included Student t test to detect differences in mean values of the variable between both sexes. Age impact was investigated by ANOVA. A total of 806 elderly people were interviewed. The mean values of the all variables presented differences statistically significant. The mean values of weight, stature, AMC, CC and WC were higher in men, whereas the mean values of BMI, MAC and TST were higher in women. All variables showed a decrease in average values and percentile (50th) with advanced age. The anthropometric and body composition data of the elderly in the present study showed similar trend of others studies, but with different values. Thereby, such values could be useful in the anthropometric assessment of elderly people.

INTRODUÇÃO: As serpentes Bothrops e Bothropoides são responsáveis por 70% dos acidentes ofídicos ocorridos no Brasil. As espécies causadoras de acidentes na Paraíba são Bothropoides erythromelas, Bothrops leucurus e Bothropoides neuwiedi. MÉTODOS: Tratou-se de um estudo prospectivo e transversal, com abordagem quantitativa dos acidentes botrópicos e botropóidicos atendidos e registrados pelos Centros de Assistência e Informação Toxicológica de Campina Grande e João Pessoa (Ceatox-CG e Ceatox-JP), com o objetivo de conhecer o perfil epidemiológico e clínico deste agravo. Todos os pacientes atendidos tiveram diagnóstico médico e acompanhamento pelos Ceatox CG e JP. RESULTADOS: Os gêneros Bothrops e Bothropoides foram responsáveis por 91,7% dos acidentes ofídicos notificados. Atingindo frequentemente o sexo masculino (75,1%), trabalhadores rurais (65,1%), alfabetizados (69%) e na faixa etária entre 11 a 20 anos (21,7%), os dedos dos pés foram as regiões anatômicas mais acometidas (52,7%). A maioria (86,6%) dos pacientes foi atendida em até 6 horas após o acidente/picada com predominância de casos leves (64,6%). A incidência anual no estado foi 5,5 acidentes/100.000 habitantes e a letalidade 0,2%. CONCLUSÕES: Verificaram-se mudanças positivas no perfil desses acidentes, como a não utilização de medidas consideradas inadequadas (uso de torniquetes, borra de café, alho, incisão com sucção e outras). Além disso, o projeto Laboratório Itinerante, ligado a Universidade Estadual da Paraíba em parceria com o Ceatox-CG, tem contribuído positivamente, levando a várias cidades do estado informações sobre prevenção e tratamento dos acidentes causados por animais peçonhentos. A imprensa local também tem contribuído, divulgando esse trabalho educativo.

INTRODUCTION: Bothrops and Bothropoides snakes cause 70% of the ophidic accidents in Brazil. The species that cause ophidic accidents in State of Paraíba are Bothropoides erythromelas, Bothrops leucurus and Bothropoides neuwiedi. METHODS: This is a prospective and transverse study, following a quantitative approach of accidents involving Bothrops and Bothropoides admitted to the Toxicological Assistance and Information Centers of Campina Grande and João Pessoa (Ceatox-CG and Ceatox-JP), aimed at identifying the epidemiological and clinical profile of such accidents. All of the patients admitted had medical diagnoses and were monitored at Ceatox-CG or Ceatox-JP. RESULTS: The genera Bothrops and Bothropoides caused 91.7% of the ophidic accidents reported. Snake bites were frequent in men (75.1%), rural workers (65.1%), literate individuals (69%) between 11 and 20 years-old (21.7%), and toes the most common area attacked (52.7%). Most (86.6%) patients were admitted within 6 hours after the accident/bite, with a predominance of mild cases (64.6%). The annual occurrence in Paraíba was 5.5 accidents/100,000 inhabitants and lethality was 0.2%. CONCLUSIONS: Positive changes in the profiles of these accidents were verified, such as the non-application of inadequate solutions, including the use of tourniquet, coffee grounds, garlic, suction and/or cutting the bitten area. Moreover, the Itinerant Laboratory project, linked to Paraíba State University in partnership with Ceatox-CG, has contributed positively, providing several cities of the state with information regarding the prevention of accidents involving venomous animals. The local press has also contributed, reporting the educational work developed by the centers.