A aceitação de tratamentos enriquecidos com óleo de tungue e a incorporação do ácido linolênico conjugado (CLnA) em filés de tilápia melhorada geneticamente (GIFT) foram investigadas. A dieta teve boa aceitação e, após 10 dias, o ClnA havia sido incorporado nos filés, mostrando um teor de 1,02% dos ácidos graxos totais (AG). Além disso, a biossíntese dos isômeros do ácido linoleico conjugado - CLA (0,31% de teor de AG total no filé), a partir do CLnA, e a presença do ácido alfalinolênico - LNA (1,08% de teor de AG total no filé), ácido eicosapentaenoico - EPA (2,85 % de teor de AG total no filé) e ácido docosa-hexaenoico - DHA (3,08% de teor de AG total no filé) foram observados. Assim, o consumo deste peixe pode aumentar a ingestão de diferentes AG (CLnA, CLA, LNA, EPA e DHA), que desempenham um papel importante no metabolismo humano.

The acceptance of tung oil enriched diet and the incorporation of conjugated linolenic acid - CLnA into fillets of Genetically Improved Farmed Tilapia (GIFT) were investigated. The diet was well accepted, and after 10 days CLnA was incorporated into the fillets with a 1.02% content of total fatty acids (FA). In addition, biosynthesis of the conjugated linoleic acid isomers - CLA (0.31% of fillet total FA content) from CLnA, and the presence of alpha-linolenic acid - LNA (1.08% of fillet total FA content), eicosapentaenoic acid - EPA (2.85% of fillet total FA content) and docosahexaenoic acid - DHA (3.08% of fillet total FA content) were observed. Therefore, the consumption of this fish can increase the intake of different FA (CLnA, CLA, LNA, EPA and DHA), which play an important role in human metabolism.

The carrot leaf dehydration conditions in air circulation oven were optimized through response surface methodology (RSM) for minimizing the degradation of polyunsaturated fatty acids, particularly alpha-linolenic (LNA, 18:3n-3). The optimized leaf drying time and temperature were 43 h and 70 ºC, respectively. The fatty acids (FA) were investigated using gas chromatography equipped with a flame ionization detector and fused silica capillary column; FA were identified with standards and based on equivalent-chain-length. LNA and other FA were quantified against C21:0 internal standard. After dehydration, the amount of LNA, quantified in mg/100 g dry matter of dehydrated carrot leaves, were 984 mg.