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SUMMARY OBJECTIVE: Osteoporosis, defined as a systemic skeletal disease, is characterized by increased bone fragility and fracture risk. Studies have shown that dysregulation of the functions of miRNAs or the mechanisms they mediate may be an important pathological factor in bone degeneration. Therefore, the aim of the study was to determine the role of miRNAs, which are thought to play a role in bone metabolism, in osteoporosis. METHODS: The study included 48 patients who were diagnosed with osteoporosis according to the results of a bone mineral density assessment by quantitative computed tomography and 36 healthy individuals. MiRNAs from plasma samples obtained from blood samples taken into ethylenediaminetetraacetic acid (EDTA) tubes were isolated with the miRNA isolation kit and converted to cDNA. Expression analysis of miR-21-5p, miR-34a-5p, miR-210, miR-122-5p, miR-125b-5p, miR-133a, miR-143-3p, miR-146a, miR-155-5p, and miR-223 was performed on the real-time PCR (RT-PCR) device. RESULTS: When miRNA expression levels in the patient group were compared with the control group, all miRNAs were found to be downregulated in the patients. When fold changes in expression levels in the patient group were examined, significant differences were found in miR-21-5p, miR-133a, mir143-3p, miR-210, and miR-223. In the receiver operating curve analysis, area under the curve=0.882 for the combination of miR-34, miR-125, miR-133, and miR-210. CONCLUSION: In this study, it was determined that the combined effects of miRNAs, as well as their single effects, were effective in the development of osteoporosis. Therefore, a miRNA panel to be created can make a significant contribution to the development of novel diagnostic and treatment approaches for this disease. OBJECTIVE Osteoporosis disease risk degeneration Therefore metabolism METHODS 4 3 individuals EDTA (EDTA cDNA miR215p, miR215p miRp miR 21 5p, 5p p miR-21-5p miR34a5p, miR34a5p miRap 34a miR-34a-5p miR210, miR210 210, 210 miR-210 miR1225p, miR1225p 122 miR-122-5p miR125b5p, miR125b5p miRbp 125b b miR-125b-5p miR133a, miR133a miRa 133a, 133a miR-133a miR1433p, miR1433p 143 3p, 3p miR-143-3p miR146a, miR146a 146a, 146a miR-146a miR1555p, miR1555p 155 miR-155-5p miR223 223 miR-22 realtime real time RTPCR RT (RT-PCR device RESULTS examined mir1433p, mir1433p mirp mir143 mir mir143-3p miR223. 223. curve0882 0 882 curve=0.88 miR34, miR34 34, 34 miR-34 miR125, miR125 125, 125 miR-125 miR133, miR133 133, 133 miR-133 miR210. 210. CONCLUSION 2 miR21 miR-21 12 14 15 miR22 22 miR-2 mir14 curve088 88 curve=0.8 miR3 miR-3 miR12 miR-12 miR13 13 miR-13 miR2 1 miR- mir1 curve08 8 curve=0. miR1 miR-1 curve0 curve=0 curve=