O presente estudo comparou características hematológicas de juvenis de jundiás Rhamdia quelen diploides e triploides, um importante peixe de água doce cultivado no sul do Brasil. A morfometria hematológica dos eritrócitos foi determinada em extensões sanguíneas sob microscopia óptica comum. O sangue dos animais foi também utilizado para mensurar o número total de eritrócitos em câmera de Neubauer, bem como os números totais de leucócitos e trombócitos pelo método indireto. Os resultados demonstraram que a triploidia aumentou (p < 0,01) o tamanho e volume dos eritrócitos. No entanto, como esperado, a triploidia reduziu (p < 0,01) o número de eritrócitos, leucócitos e trombócitos circulantes do jundiá. Além disto, a contagem diferencial de células sanguíneas foi diferente em diploides e triploides, sugerindo que a triploidia afeta o número das células, bem como sua proporção no sangue. Linfócitos foram as células predominantes na contagem de leucócitos de peixes diploides (62,5%), enquanto os monócitos foram os predominantes nos peixes triploides (49,6%). Estes resultados sugerem desempenhos diferentes entre as ploidias do jundiá e exige estudos futuros para avaliar o potencial dos jundiás triploides sob condições de cultivo e frente à infecção.

This study compared the hematological characteristics of diploid and triploid of jundia, Rhamdia quelen juveniles, an important freshwater fish cultured in south Brazil. Hematological morphometry of erythrocytes were determined in blood smears under a light microscope. The blood was used to measure the number of red blood cells (RBC) with a hemocytometer Neubauer chamber, and the numbers of white blood cells (WBC) and thrombocytes that were obtained using an indirect method. The results showed that triploidy increased (p < 0.01) the size and volume of the erythrocytes. Nevertheless, as expected, triploidy decreased (p < 0.01) the number of circulating erythrocytes, leucocytes and trombocytes in the blood of jundia. Moreover differential cell counts were different in diploids and triploids, suggesting that triploidy affects the number of cells and their proportion in blood. Lymphocytes were the most predominant cells in the differential counting of diploid fish (62.5%) while monocytes were predominant in triploid fish (49.6%). These results suggest performance differences between ploidies of jundia, and require future studies to evaluate the potential of triploid jundia in the culture conditions and resistance to infection.

This study evaluated the effects of polyvalent vaccination on the hematological and serum agglutination responses in Nile tilapia challenged with Aeromonas hydrophila. Two dosis, 1 x 10(4) and 1 x 10(8)) Colony Forming Units (CFU)/mL, of vaccine containing the same amount of Aeromonas hydrophila, Pseudomonas aeruginosa and Enterococcus durans formalin-inactivated were tested by intraperitoneal (i.p) injection. Fish were challenged ten days after vaccination i.p. with a DL50-96h of 1 x 10(7) CFU A. hydrophila/mL. Samples were collected 48 h after challenging fish to check the hematological parameters, antimicrobial activity and agglutination titer of serum, samples were collected 48 h after challenge. Before challenge, the number of erythrocytes was higher in fish vaccinated with 1 x 10(8) CFU/mL. After challenge, total number of thrombocytes was higher in fish that received the greatest dose of vaccine. Before and after challenge, total number of leukocytes and the number of lymphocytes showed the highest values in vaccinated fish. Before challenge, increased number of monocytes in vaccinated and saline-injected fish was observed. The highest agglutination titer against A. hydrophila, P. aeruginosa and E. durans was related in 1 x 10(8) CFU/mL vaccinated fish. Before challenge, high values of antimicrobial activity in non-vaccinated fish and 1 x 10(8) CFU/mL vaccinated ones was also related. Therefore, after challenge, non-vaccinated fish and saline-injected ones showed the highest antimicrobial activity. This study showed that 10 days after immunization with a polyvalent vaccine at a concentration 1 x 10(8) CFU/mL, there was an increase on erythrocytes, leukocytes, thrombocytes and circulating lymphocytes production, while the glucose levels were reduced.

Este trabajo evaluó el efecto de la vacuna polivalente sobre las respuestas hematológicas y inmunológicas de tilapias del Nilo desafiadas con Aeromonas hydrophila. Dos dosis, 1 x 10(4) y 1 x 10(8) Unidades Formadoras de Colonias (UFC)/mL, de vacuna conteniendo proporciones iguales de Aeromonas hydrophila, Pseudomonas aeruginosa y Enterococcus durans inactivadas con formalina, fueron testeadas por inyección intraperitoneal (i.p.). Los peces fueron desafiados 10 días después de la vacunación i.p. con la dosis (DL50-96h) de 1 x 10(7) UFC de A. hydrophila/mL resuspendida en solución salina estéril. Por lo tanto, para analizar los parámetros hematológicos, la actividad antimicrobiana y aglutinante del suero de las muestras, fueron colectadas 48 h después del desafío. Antes de la infección experimental, el número de eritrocitos fue superior en los peces vacunados con 1 x 10(8) UFC/mL. Sin embargo, después del desafío, el número total de trombocitos fue mayor en los peces vacunados con la mayor dosis. Antes y después del desafío, el número total de leucocitos y el número de linfocitos presentaron mayores valores en los peces vacunados. El número de monocitos en los peces vacunados y en los inyectados con solución salina fue mayor antes del desafío. El mayor título de aglutinación frente A. hydrophila, P. aeruginosa y E. durans fue observado en los peces vacunados con 1 x 10(8) UFC/mL. Antes del desafío la actividad antimicrobiana del suero sanguíneo fue mayor en los peces no vacunados y en los vacunados con 1 x 10(8) UFC/mL, y mismo después del desafío los peces no vacunados y los inyectados con solución salina presentaron mayor actividad antimicrobiana. En este trabajo fue posible comprobar que después de 10 días la vacuna polivalente en la concentración de 1 x 10(8) UFC/mL estimuló la producción de eritrocitos, leucocitos, trombocitos y linfocitos circulantes reduciendo los niveles de glucosa.