ABSTRACT The purpose of this study was to assess the impact of a blend of essential oils from eucalyptus, citrus, bromohexene HCl, thymole and camphor on the growth performance, carcass characteristics, meat quality, intestinal morphology, serum biochemistry, and immune response of broiler chickens. A total of 240 day-old chicks were divided into four groups, each with six replicates containing ten birds. The experiment was conducted under a completely randomized design (CRD). Different concentrations of the oil blend (0mL/kg, 0.15mL/kg, 0.30mL/kg and 0.45mL/kg) were added to the diet. The findings showed that, in comparison to the other groups, the birds that were given a blend of essential oils at concentrations of 0.30 and 0.45 mL/kg showed improved weight gain, feed efficiency, carcass yield, villus height, crypt depth, and greater immune response against Newcastle disease vaccination (p<0.05). Nonetheless, there was no statistically significant difference in the yields of the breast and thighs, feed consumption, mortality, weights of the liver, wing, heart, and gizzard, or abdominal fat between the treatments. The addition of a blend of essential oils at the doses of 0.30 and 0.45 mL/kg significantly lowered the pH of the meat in comparison to the other groups (p<0.05). All blood biochemical markers, including total serum protein, albumin, globulin, glucose, cholesterol, triglyceride, and uric acid, revealed no variations between the treatments. In conclusion, adding 0.30mL/kg of a blend of essential oils to broiler diets may be the optimum level to improve overall performance without adversely affecting the blood biochemical profile. eucalyptus citrus HCl characteristics quality morphology biochemistry chickens 24 dayold day old CRD. CRD . (CRD) 0mL/kg, 0mLkg mLkg 0mL kg mL (0mL/kg 015mLkg 0 15mL 0.15mL/kg 030mLkg 30mL 0.45mL/kg 045mLkg 45mL diet 030 30 0.3 045 45 0.4 gain efficiency yield height depth p<0.05. p005 p p<0.05 05 (p<0.05) Nonetheless thighs consumption mortality liver wing heart gizzard treatments markers protein albumin globulin glucose cholesterol triglyceride acid conclusion profile 2 (CRD 0mL/kg 03 3 0. 04 4 p00 p<0.0 (p<0.05 p0 p<0. (p<0.0 p<0 (p<0. p< (p<0 (p< (p

Resumo A administração pós-natal precoce de antibióticos tem sido associada a efeitos duradouros no desenvolvimento e comportamento do cérebro. Pesquisas conduzidas em animais livres de germes demonstraram que o impacto da colonização do microbioma na regulação do eixo hipotálamo-hipófise-adrenal (HPA) e nas vias neuroendócrinas é substancial, o que desempenha um papel crucial no gerenciamento do estresse. No entanto, ainda é incerto se a exposição a antibióticos em ratas mães (geração F0) antes do desmame está associada a mudanças neurocomportamentais em filhotes (geração F1) durante a idade adulta. Para investigar os efeitos, perturbamos a microbiota intestinal de ratas (geração F0) administrando cefixima (CEF), um antibiótico comumente usado para fins obstétricos, em doses clinicamente relevantes (1 mg/kg, 2,5 mg/kg ou 5 mg/kg). Comportamentos semelhantes à ansiedade em descendentes adultos foram avaliados por meio da utilização do labirinto em cruz elevado (EPM) e paradigma de campo aberto (OFP) após um intervalo de seis semanas a partir do nascimento (PND42). Após as avaliações comportamentais, os ratos foram sacrificados, e seus cérebros e sangue coletados para análise bioquímica. A concentração plasmática de corticosterona foi usada para avaliar a atividade do HPA, enquanto a reação em cadeia da polimerase quantitativa em tempo real (PCR) foi empregada para determinar os níveis de transcrição do receptor de glicocorticoide (GR) Nr3c1. Os descendentes de F1 que receberam antibióticos antes do desmame passaram menos tempo no braço aberto do EPM. As alterações foram acompanhadas por níveis aumentados de corticosterona na corrente sanguínea. O estudo da expressão gênica revelou diminuição nos níveis de transcrição do mRNA de Nr3c1. Esta pesquisa enfatiza os possíveis efeitos a longo prazo da exposição a antibióticos antes do desmame no desenvolvimento de ansiedade na prole na idade adulta. pósnatal pós natal cérebro hipotálamohipófiseadrenal hipotálamo hipófise adrenal HPA (HPA substancial estresse entanto geração F0 F adulta CEF, CEF , (CEF) obstétricos 1 ( mgkg mg kg 25 2 2, mg/kg. . mg/kg) EPM (EPM OFP (OFP PND42. PND42 PND (PND42) comportamentais sacrificados bioquímica PCR (PCR GR (GR Nr3c1 Nrc Nr c sanguínea (CEF PND4 (PND42 Nr3c (PND4 (PND

Abstract Early postnatal administration of antibiotics has been linked to lasting effects on brain development and behavior. Research conducted on animals that are free from germs has demonstrated that the impact of microbiome colonization on the regulation of the hypothalamic-pituitary-adrenal (HPA) axis and neuroendocrine pathways is substantial, which play a crucial role in stress management. Nevertheless, it is still uncertain if the exposure to antibiotics in rat dams (F0-generation) before weaning is associated with neurobehavioral changes in rat offspring (F1-generation) during adulthood. In order to investigate the effects, we perturbed the intestinal microbiota of rat dams (F0 generation) by administering cefixime (CEF), an antibiotic commonly used for obstetric purposes, at clinically relevant doses (1 mg/kg, 2.5 mg/kg or 5 mg/kg). Anxiety-like behaviors in adult offspring was evaluated through the utilization of elevated plus maze (EPM) and open field paradigm (OFP) following a six-week interval from birth (PND42). Subsequent to behavioral assessments, the rats were euthanized, and their brains and blood was collected for biochemical analysis. Plasma corticosterone concentration was used to assess HPA activity, whereas the quantitative real-time polymerase chain reaction (PCR) was employed to determine the transcription levels of the glucocorticoid receptor (GR) Nr3c1. The offspring of F1 that were administered antibiotics before being weaned spent less time in the EPM open arm. The alterations were accompanied by increased levels of corticosterone in the bloodstream. The gene expression study revealed a decrease in the levels of mRNA transcription of Nr3c1. This research emphasizes the possible long-term effects of antibiotic exposure before weaning on the development of anxiety in offspring upon adulthood. behavior hypothalamicpituitaryadrenal hypothalamic pituitary adrenal (HPA substantial management Nevertheless F0generation Fgeneration F0 generation F (F0-generation F1generation (F1-generation adulthood (F CEF, CEF , (CEF) purposes 1 ( mgkg mg kg 25 2 2. mg/kg. . mg/kg) Anxietylike Anxiety like (EPM OFP (OFP sixweek six week PND42. PND42 PND (PND42) assessments euthanized analysis activity realtime real PCR (PCR GR (GR Nr3c1 Nrc Nr c arm bloodstream longterm long term (CEF PND4 (PND42 Nr3c (PND4 (PND

ABSTRACT The goal of the current study was to determine how selenium-supplemented diets affected the growth performance, carcass traits, and blood biochemistry of Rhode Island Red (RIR) chickens. Organic and inorganic selenium (SE) was fed to the birds at 0.30ppm, whereas the control diet was not supplemented with selenium in either organic or inorganic form. A total of 225 day-old RIR chicks were indiscriminately distributed in three groups according to the experimental diets, in a completely randomized design. Each treatment group was repeated 5 times (replicates), and each replicate had 15 birds. Parameters of growth performance, carcass characteristics, and blood biochemistry were assessed. Birds fed an inorganic selenium-supplemented diet had higher feed intake than those fed an organic selenium-supplemented diet or the control diet, whereas birds fed an organic selenium-supplemented diet had higher body weight gains and better feed conversion ratios. Birds fed organic SE in the diet showed higher breast and thigh weight than those receiving inorganic Se, whereas dressing percentage, drumstick yield, and weights of liver, gizzard, heart, and wing were not significantly different across treatments. The organic Se group showed higher values for total protein and globulin in the blood relative to those fed inorganic Se and the control diet. Similarly, organic Se fed birds showed higher blood Se concentration than the other two groups. However, no significant differences in albumin, glucose, cholesterol, triglycerides, and uric acid were observed among the diets. These results lead to the conclusion that organic Se may be utilized in diets to improve the poor performance of RIR chicken. seleniumsupplemented traits (RIR chickens (SE 030ppm ppm 0 30ppm 0.30ppm form 22 dayold day old design replicates, replicates , (replicates) 1 characteristics assessed ratios percentage yield liver gizzard heart treatments Similarly However albumin glucose cholesterol triglycerides chicken 2 (replicates

Resumo A indústria avícola gera muitos resíduos, incluindo aves mortas, esterco e cama de frango. Resíduos de aves nunca devem ser descartados de forma inadequada, pois podem prejudicar seriamente o meio ambiente. Os resíduos podem ser reciclados como matéria-prima para uso na alimentação de aves, compostando a cama e as aves mortas. O perfil de composição e as propriedades organolépticas da carne de codornas japonesas em crescimento foram examinados ao longo de um ensaio de 4 semanas para verificar o efeito da adição de composto à dieta. Em um delineamento inteiramente casualizado (CRD), 1.200 pintos de codorna recém-eclodidos (Coturnix coturnix japonica) foram divididos em cinco grupos de tratamento (dietas com 0, 2,5, 5, 7,5 e 10% de composto), cada um composto por 40 aves com seis repetições. A adição de composto à dieta não teve efeitos perceptíveis nas qualidades organolépticas de aparência, cor, aroma, sabor, textura, suculência, maciez e aceitabilidade (P > 0,05). As características do perfil de composição para pintos que receberam composto em qualquer nível comparadas aos pintos alimentados com a dieta controle não mostraram diferenças (P > 0,05). Essas descobertas sugerem que as características sensoriais e o perfil de composição de codornas de corte em crescimento podem ser mantidos quando alimentadas com dietas incluindo até 10% de composto. mortas frango inadequada ambiente matériaprima matéria prima CRD, CRD , (CRD) 1200 1 200 1.20 recémeclodidos recém eclodidos Coturnix japonica 0 25 2 5 2,5 75 7 7, 10 composto, composto) repetições aparência cor aroma sabor textura suculência P 0,05. 005 0,05 . 05 0,05) (CRD 120 20 1.2 2, 00 0,0 12 1.

Abstract The poultry industry generates a lot of waste, including dead birds, manure, and poultry litter. Poultry waste should never be disposed of improperly because it can seriously harm the environment. The waste can be recycled as a feedstock for use in poultry feed by composting the litter and deceased birds. The compositional profile and organoleptic properties of the meat of growing Japanese quail were examined over the course of a 4-week trial to ascertain the effect of adding compost to the diet. In a completely randomized design (CRD), 1200 newly hatched quail chicks (Coturnix coturnix japonica) were divided into five treatment groups (diets with 0, 2.5, 5, 7.5, and 10% compost), each consisting of 40 birds with six replicates. The addition of compost to the diet had no noticeable effects on the organoleptic qualities of appearance, color, aroma, taste, texture, juiciness, tenderness, and acceptability (P>0.05). The compositional profile characteristics for chicks given compost at any level compared to chicks fed the control diet showed no differences (P>0.05). These findings suggest that the sensory characteristics and compositional profile of growing meat quails can be maintained when fed diets including up to 10% compost. manure environment 4week week 4 CRD, CRD , (CRD) 120 Coturnix japonica 0 25 2 5 2.5 75 7 7.5 10 compost, compost) replicates appearance color aroma taste texture juiciness tenderness P>0.05. P005 P P>0.05 . 05 (P>0.05) (CRD 12 2. 7. 1 P00 P>0.0 (P>0.05 P0 P>0. (P>0.0 P>0 (P>0. P> (P>0 (P> (P

Resumo Grandes quantidades de resíduos, incluindo aves mortas, esterco e cama de frango, são produzidas pela indústria avícola. Resíduos de aves devem ser descartados adequadamente para evitar grandes riscos de poluição e saúde. A compostagem de lixo e aves mortas pode ser uma opção para reciclar os resíduos e usá-los na alimentação de aves. Um estudo foi conduzido para investigar os efeitos da alimentação com resíduos de aves compostados nas qualidades organolépticas e no perfil de composição da carne de frangos de corte. Um total de 300 pintos de corte de um dia (500-Cobb) foram alocados aleatoriamente em 5 grupos de tratamento, replicados 6 vezes com 10 aves cada, sob um delineamento inteiramente casualizado (CRD). Cinco dietas isocalóricas e isonitrogenadas incluindo subprodutos compostados de aves nas concentrações de 0, 2,5, 5, 7,5 e 10% foram fornecidas ad libitum às aves do dia 0 ao dia 35. A classificação sensorial e o perfil de composição da carne de 500 frangos de corte Cobb foram testados aos 35 dias de idade. Os achados mostraram que não houve variações nos perfis sensoriais da carne de aves recebendo várias dietas (P > 0,05). Embora os resultados tenham sido um pouco menores para os pintos alimentados com dietas contendo composto do que para o grupo controle, essa diferença foi considerada insignificante (P > 0,05). Da mesma forma, não houve variações nos valores do perfil composicional da carne entre carnes de aves alimentadas com várias dietas (P > 0,05). Essas descobertas indicam que os frangos de corte podem ser criados com dietas contendo até 10% de composto de subproduto de aves sem qualquer impacto negativo na qualidade sensorial ou na composição da carne. Além disso, o uso de composto nas dietas de frangos de corte pode ajudar a reduzir o custo da ração. frango avícola saúde usálos usá los 30 500Cobb (500-Cobb tratamento 1 cada CRD. CRD . (CRD) 25 2 2,5 75 7 7, 50 3 idade P 0,05. 005 0,05 05 0,05) controle forma disso ração (CRD 2, 00 0,0

Abstract Large amounts of waste, including dead birds, manure, and poultry litter, are produced by the poultry industry. Poultry waste should be disposed of properly to avoid major pollution and health risks. Composting litter and dead birds could be an option to recycle the waste and use in poultry feed. A study was conducted to investigate the effects of feeding composted poultry waste on the organoleptic qualities and compositional profile of the meat of broiler chickens. A total of 300 day-old broiler chicks (500-Cobb) were randomly allocated to five treatment groups replicated six times with 10 birds each, under a completely randomized design (CRD). Five iso-caloric and iso-nitrogenous diets including composted poultry byproducts at concentrations of 0, 2.5, 5, 7.5, and 10% were fed ad libitum to the birds from day 0 to day 35. The sensory grading and meat composition profile of 500 Cobb broiler chickens were tested at 35 days of age. The findings showed that there were no variations in the sensory profiles of the meat from birds given various diets (P>0.05). Although the results were somewhat lower for the chicks fed compost-containing diets than for the control group, this difference was deemed to be insignificant (P>0.05). Similarly, there were no variations in the compositional profile values of the meat between meat from birds fed various diets (P>0.05). These findings imply that broiler chickens may be raised on diets containing up to 10% poultry byproduct compost without any negative impacts on the meat's sensory quality or composition. Additionally, using compost into broiler diets may help to lower the cost of feed. manure industry risks feed 30 dayold old 500Cobb (500-Cobb 1 each CRD. CRD . (CRD) isocaloric iso caloric isonitrogenous nitrogenous 25 2 5 2.5 75 7 7.5 50 3 age P>0.05. P005 P P>0.05 05 (P>0.05) compostcontaining group Similarly meats s Additionally (CRD 2. 7. P00 P>0.0 (P>0.05 P0 P>0. (P>0.0 P>0 (P>0. P> (P>0 (P> (P

Resumo O presente estudo explicou o efeito de sistemas alternativos de produção sobre o crescimento, características morfométricas e carcaças de quatro genótipos de frango diferentes. A segunda geração de dois genótipos RNN (Rhode Island Red × Naked Neck) e BNN (Black Australorp × Naked Neck) obtida por duas autocruzes (RNN × RNN = RR e B ANN × BNN = BB) e duas cruzes recíprocas (RNN × BNN = RB e BNN × RNN = BR) foi avaliada em três sistemas de produção alternativos (gaiolas convencionais, gaiolas enriquecidas e aviário). Na 6ª semana de idade após o sexo, um total de 600 aves, compostas por 150 de cada raça cruzada com um total de 300 pullets e 300 galos, foi dividido em gaiolas convencionais, gaiolas enriquecidas e sistemas aviários com 200 aves em cada. As aves foram organizadas em 3×4 arranjos fatoriais sob projeto completamente randomizado (3 sistemas de produção × 4 genótipos × 2 sexos × 25 aves = 600 aves). Em relação aos genótipos, os machos RB e BR apresentaram maior rendimento de carcaça (p < 0,01), peso da baqueta, peso mamário e peso da coxa do que os genótipos BB e RR. As fêmeas do genótipo BR apresentaram maior (p < 0,01) peso mamário, peso da coxa e peso da baqueta. No que diz respeito aos sistemas de produção, maior (p < 0,01) peso hepático, peso cardíaco, peso mamário, peso intestinal, peso da baqueta e peso da coxa foram observados nos machos criados em gaiolas enriquecidas em comparação com gaiolas convencionais e sistema aviário. As fêmeas criadas em gaiolas enriquecidas apresentaram maior (p < 0,01) peso cardíaco, peso mamário, peso intestinal, peso da baqueta e peso da coxa quando comparadas com as criadas em gaiolas convencionais e aviárias. Conclui-se que as galinhas (ambos os sexos) dos genótipos BR e RB apresentaram melhores medidas morfológicas e traços de carcaça do que os de frangos genótipos RR e BB. Entre os sistemas de produção alternativos, as galinhas criadas em gaiolas enriquecidas tinham características melhores do que as criadas em gaiolas convencionais e aviárias durante a fase de cultivo.

Abstract The present trial explained the effect of alternative production systems on growth, morphometric and carcass traits of four different chicken genotypes. The second generation of two genotypes RNN (Rhode Island Red × Naked Neck) and BNN (Black Australorp × Naked Neck) obtained by two self-crosses (RNN × RNN = RR and BNN × BNN = BB) and two reciprocal crosses (RNN × BNN = RB and BNN × RNN = BR) were evaluated in three alternative production systems (conventional cages, enriched cages, and aviary). At the 6th week of age after sexing, a total of 600 birds, comprising 150 from each crossbred with a total of 300 pullets and 300 cockerels were divided into conventional cages, enriched cages, and aviary systems having 200 birds in each.Birds were organized into 3×4 factorial arrangements under Completely Randomized Design (3 production systems × 4 genotypes × 2 sexes × 25 birds = 600 birds). Regarding genotypes, RB and BR males showed higher (p < 0.01) carcass yield, drumstick weight, breast weight, and thigh weight than BB and RR genotypes. Females of BR genotype showed higher (p < 0.01) breast weight, thigh weight and drumstick weight. As far as production systems are concerned, higher (p < 0.01) liver weight, heart weight, breast weight, intestinal weight, drumstick weight, and thigh weight were observed in the males reared in enriched cages compared with conventional cages and aviary system. Females reared in enriched cages showed higher (p < 0.01) heart weight, breast weight, intestinal weight, drumstick weight, and thigh weight when compared with those reared in conventional cages and aviary. It is concluded that chickens (both sexes) of BR and RB genotypes had better morphological measurements and carcass traits than those of RR and BB genotype chickens. Among alternative production systems, chickens reared in enriched cages had better traits than those of reared in conventional cages and aviary during the growing phase.

ABSTRACT The aim of the present study was to evaluate the effect of different anti-stressors on growth performance, carcass traits, bloodchemistry and meat quality attributes of broiler Japanese quail. For this purpose, a total of 1,875 quail chicks were procured and arranged according to completely randomized design, birds were divided into 5 treatment groups having 5 replicates of 75 birds each. The treatments consisted of four anti-stressors i.e., synthetic anti-stressors (Vitamin C and Betaine) and natural anti-stressors (Turmeric and Chia Seed) along with a control group. Bird’s diets were supplemented with vitamin C, betaine, turmeric and chia seed @ 600 mg/kg, 700 mg /kg, 300 mg/kg and 7,000 mg/kg, respectively. After four weeks, 10 birds per treatment were slaughtered (Halal method), de-feathered, eviscerated and their carcass traits, serum chemistry and meat quality attributes were calculated. Birds supplemented with betaine revealed significantly (p≤0.05) lower feed intake, glucose, cholesterol, H:L ratio, better weight gain, times of gain, FCR and highest anti-oxidant assay and total viable count. However, carcass traits, processing traits and blood cortisol level did not differ (p>0.05) among the treatment groups. In conclusion, addition of anti-stressors (especially betaine) in the diet of growing Japanese quail had positive influence on growth and serum chemistry.

ABSTRACT The study was planned to evaluate the effect of different naked-neck (NN) chicken genotypes and breeder ages on the growth performance of their progenies. A total of 126 chicks, from two breeder ages and three NN varieties, were reared up to 20 weeks of age. A randomized complete block design in factorial arrangement, with six treatments of seven replicates of two females and one male each, was applied. Treatments consisted of three NN varieties (light brown, black, and dark brown) and two breeder age groups (45 and 55 weeks old). Growth performance (body weight gain, feed intake and feed conversion ratio) were evaluated. Higher feed intake was observed in both the male and female progenies of 55-week-old compared with 45-week-old breeders. Light brown males had higher weight gain than dark brown males, whereas in the female progeny, higher weight gain was obtained in the black variety than that in the light brown and dark brown varieties. Regarding breeder age groups, both male and female progenies of 55-week-oldbreeders exhibited higher weight gain than those of 45-week-old breeders. The male progeny of 55-week-old black breeders showed the highest body weight gain among all treatments. Regarding female progeny, higher weight gain was observed in the black variety compared with the light brown and dark brown variety, and in the progeny of 55-week-old breeders than in the progeny of 45-week-old breeders. The interaction between factors showed that females from 55-week-old black breeder has the highest weight gain, whereas the lowest weight gain was found in the progeny of 45-week-old light brown breeders. The male progeny of light brown breeders showed better FCR than those of black and dark brown breeders and of 55-week-old breeders compared with that of 45-week-old breeders. The best FCR was obtained in the progeny of 55-week-old light brown breeders. In females, while no influence of breeder age was detected, better FCR was determined in black than in dark brown and light brown varieties; however, the interaction between factors was significant, with the best FCR obtained in the female progeny of 55-week-old black breeders, and worst in the progeny of 45-week-old light brown breeders. In conclusion, the light brown and black varieties of naked-neck chickens and the progeny of 55-week-old breeders presented better growth performance.