Abstract The methanolic, chloroformic and aqueous extract of Achillea millefolium and Chaerophyllum villosum were investigated for cytotoxicity, phytotoxic and insecticidal activities. Cytotoxicity was investigated by brine shrimp lethality assay indicating that the crude methanolic extract of A.millefolium and chloroformic extract of C.villosum revealed highest mortality of brine shrimps with (LD50 of 52.60 µg/ml) and (14.81 µg/ml). Phytotoxicity was evaluated using the Lemna minor bioassay which revealed that the crude methanolic extract of A.millefolium and C.villosum extract has maximum inhibition of Lemna minor with (Fl50 6.60 µg/ml) and (0.67 µg/ml).The insecticidal activity showed that among all the insects studied it was observed that methanolic extract of A. millefoliumand C. villosum was highly toxic to Sphenoptera dadkhani with (LD50=4.17 µg/ml) and (0.34 µg/ml). From the present study it can be concluded that different extracts from A. millefolium and C. villosum showed good cytotoxic, phytotoxic and insecticidal activity in a dose dependent manner. cytotoxicity activities Amillefolium A Cvillosum C LD50 LD (LD5 5260 52 60 52.6 µg/ml µgml µg ml 14.81 1481 14 81 (14.8 µg/ml. . Fl50 Fl (Fl5 660 6 6.6 0.67 067 0 67 (0.6 µg/ml.The µgmlThe .The LD50=4.17 LD50417 4 17 (LD50=4.1 0.34 034 34 (0.3 cytotoxic manner LD5 (LD 526 5 52. 14.8 148 1 8 (14. Fl5 (Fl 66 6. 0.6 06 (0. LD50=4.1 LD5041 (LD50=4. 0.3 03 3 14. (14 0. (0 LD50=4. LD504 (LD50=4 (1 ( LD50=4 (LD50= LD50=

Resumo Neste estudo, os extratos metanólico, clorofórmico e aquoso de Achillea millefolium e Chaerophyllum villosum foram analisados em relação à citotoxicidade, atividade fitotóxica e inseticida. A citotoxicidade foi analisada através do ensaio de letalidade de artémia, indicando que o extrato metanólico bruto de A. millefolium e o extrato clorofórmico de C. villosum revelaram maior mortalidade de artêmias com DL50 de 52,60 µg/ml e 14,81 µg/ml. A fitotoxicidade foi avaliada utilizando o bioensaio de Lemna minor que revelou que o extrato metanólico bruto de A. millefolium e extrato de C. villosum têm inibição máxima de Lemna minor com Fl50 6,60 µg/ml e 0,67 µg/ml. A atividade inseticida mostrou que dentre todos os insetos estudados, o extrato metanólico de A. millefolium e de C. villosum foi altamente tóxico para Sphenoptera dadkhanicom DL50 = 4,17 µg/ml e 0,34 µg/ml . Por outro lado, diferentes extratos, como A. millefolium e C. villosum apresentaram boa atividade citotóxica, fitotóxica e inseticida de forma dose-dependente. estudo artémia C DL DL5 5260 52 60 52,6 µgml µg ml 1481 14 81 14,8 Fl Fl5 660 6 6,6 067 0 67 0,6 estudados 417 4 17 4,1 034 34 0,3 lado citotóxica dosedependente. dosedependente dose dependente. dependente dose-dependente 526 5 52, 148 1 8 14, 66 6, 06 0, 41 4, 03 3

Resumo Chrozophora tinctoria (L.) A.Juss. é uma planta anual herbácea, monoica, usada tradicionalmente para curar distúrbios gastrointestinais. O presente estudo foi realizado para encontrar os compostos bioativos por Cromatografia Gasosa-Espectrometria de Massa (GC-MS), a toxicidade aguda do potencial inibitório da acetilcolinesterase e a atividade emética presente na fração acetato de etila de Chrozophora tinctoria (EAFCT) e fração diclorometano de Chrozophora tinctoria (DCMFCT). Os compostos detectados em ambas as frações foram principalmente ácidos graxos, com cerca de sete compostos em EAFCT e 10 em DCMFCT. Estes incluíam compostos farmacologicamente ativos, como a imipramina, usada para tratar a depressão, ou éster metílico do ácido hexadecanoico, um antioxidante, nematicida, pesticida, hipocolesterolêmico, ácido 9,12,15-octadecatrienoico, éster etílico, (Z,Z,Z)- é usado como preventivo do câncer, antiartrítico, anti-histamínico, hepatoprotetor, inseticida, nematicida, ácido pentadecanoico, 14-metil-, éster metílico tem atividades antifúngicas, antimicrobianas e antioxidantes, ácido 10-octadecanoico, éster metílico tem a propriedade de diminuir o colesterol no sangue, antioxidante e antimicrobiano, o 1-Eicosanol é usado como antibacteriano, o 1-Hexadeceno possui atividades antibacteriana, antioxidante e antifúngica. Ambas as frações DCMFCT e EAFCT inibiram a atividade da acetilcolinesterase (AChE) com valores de IC50 de 10µg e 130µg, respectivamente. Ambas as frações foram consideradas tóxicas de maneira dose-dependente, induzindo vômitos a partir de 0,5g e letargia e mortalidade de 3g a 5g para cima. Ambas as frações combinadas com água destilada apresentaram atividade altamente emética. O aumento significativo no número de vômitos foi demonstrado pelo EAFCT mais água destilada, que são 7,50±1,29, 7,25±3,10 e 11,75±2,22 número de vômitos nas concentrações de 1g, 2g e 3g/kg, respectivamente, enquanto DCMFCT mais água destilada mostrou 5,25±2,22, 7,50±2,52 e 10,25±2,22 número de vômitos em 1g, 2g e 3g/kg, respectivamente. A droga padrão antiemética metoclopramida tem um impacto maior contra a emese induzida por ambas as frações do que o dimenidrinato. A metoclopramida diminui o número de vômitos causados por EAFCT para 1,00±0,00, 2,00±0,00, 4,00±1,00 a 1g, 2g e 3g/kg, sequencialmente, enquanto o dimenidrinato diminui o número de vômitos para 1,33±0,58, 2,33±1,15, 4,33± 0,58 a 1g, 2g e 3g, respectivamente. Da mesma forma, a metocloprimida diminui o número de vômitos causados por DcmCt de 5,25±2,22, 7,50±2,52, 10,25±2,22 para 1,33±0,58, 2,33±1,1, 4,33±0,58 nas concentrações de 1g, 2g e 3g/kg. O presente estudo é o primeiro relato documentado que valida cientificamente o uso folclórico de Chrozophora tinctoria como agente emético.

Abstract Chrozophora tinctoria (L.) A.Juss. is herbaceous, monecious annual plant used traditionally to cure gastrointestinal disorders. The present study was carried out to find the bioactive compounds by Gas Chromatography-Mass Spectrometry, the acetylcholinesterase inhibitory potential acute toxicity, and emetic activity present in the ethyl acetate fraction of Chrozophora tinctoria (EAFCT) and dichloromethane fraction of Chrozophora tinctoria (DCMFCT). The compounds detected in both fractions were mostly fatty acids, with about seven compounds in EAFCT and 10 in DCMFCT. These included pharmacologically active compounds such as imipramine, used to treat depression, or hexadecanoic acid methyl ester, an antioxidant, nematicide, pesticide, hypocholesterolemic, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z)- is used as a cancer preventive, antiarthritic, antihistaminic, hepatoprotective, insectifuge, nematicide, Pentadecanoic acid, 14-methyl-, methyl ester have antifungal, antimicrobial and antioxidant activities, 10-Octadecanoic acid, methyl ester have the property to decrease blood cholesterol, Antioxidant and antimicrobial, 1-Eicosanol is used as an antibacterial, 1-Hexadecene has antibacterial, antioxidant, and antifungal activities. Both DCMFCT and EAFCT fractions inhibited acetylcholinesterase (AChE) activity with IC50 values of 10 µg and 130 µg, respectively. Both the fractions were found to be toxic in a dose-dependent manner, inducing emesis at 0.5g onward and lethargy and mortality from 3–5 g upwards. Both the fractions combined with distilled water showed highly emetic activity. The significant increase in the number of vomits was shown by EAFCT plus distilled water which are 7.50±1.29, 7.25±3.10, and 11.75±2.22 number of vomits at 1g, 2g, and 3g/kg concentration respectively, while DCMFCT plus distilled water showed 5.25±2.22, 7.50±2.52 and 10.25±2.22 number of vomits at 1g, 2, and 3g/kg correspondingly. The antiemetic standard drug metoclopramide has a higher impact against the emesis induced by both the fractions than dimenhydrinate. Metoclopramide decreases the number of vomits caused by EAFCT to 1.00±0.00, 2.00±0.00, 4.00±1.00 at 1g, 2, and 3g/kg sequentially, while dimenhydrinate decreases the number of vomits to 1.33±0.58, 2.33±1.15, 4.33±0.58 at 1g, 2, and 3g respectively. In the same way, Metochloprimide decreases the number of emesis caused by DcmCt from 5.25±2.22, 7.50±2.52, 10.25±2.22 to 1.33±0.58, 2.33±1.1, 4.33±0.58 at 1g, 2, and 3g/kg concentrations. The present study is the first documented report that scientifically validates the folkloric use of Chrozophora tinctoria as an emetic agent.

ABSTRACT The selection of an appropriate production system plays a crucial role in ensuring the health, welfare, and productivity of American Pekin ducks. The aim of this study was to investigate the influence of various rearing production systems on the performance of American Pekin Ducks. A total of 180 straight run ducklings (10 days old) were randomly allocated into three experimental groups: intensive production system (IPS), free-range production system (FRPS), and pool with yard production system (PYPS). The distribution was carried out following a completely randomized design. We investigated the morphological parameters, growth performance, serum chemistry, meat cholesterol and triacylglycerol, cellular immunity, intestinal morphology, and carcass traits of Pekin ducks. The results indicate that PYPS birds exhibited significantly better morphometric parameters compared to IPS and FRPS ones. Ducks raised in PYPS had the highest growth rate till the 75th day as compared to those reared in IPS and FRPS. Triacylglycerol, total lipids, blood and meat fat and triacylglycerol were higher in IPS; whereas albumin, AG ratio, total proteins, glucose, and globulin were higher in PYPS. Similarly, cellular immunity (phagocytic activity, eosinophils, basophils, and monocytes) was increased in PYPS. Intestinal morphology and overall carcass traits were better in birds reared under PYPS than in those of IPS and FRPS. American Pekin ducks raised in PYPS showed improved growth, serum chemistry, cellular immunity, and carcass traits. These results suggest that the PYPS could be a promising production system for raising ducks in sub-tropical regions. health welfare 18 10 (1 old groups IPS, , (IPS) freerange free range FRPS, (FRPS) . (PYPS) design chemistry ones th Triacylglycerol lipids albumin ratio proteins glucose Similarly phagocytic activity eosinophils basophils monocytes subtropical sub tropical regions 1 ( (IPS (FRPS (PYPS

RESUMO Fatores climáticos desempenham papel essencial no crescimento da largura dos anéis das árvores. Neste estudo, objetivou-se avaliar a correlação entre variáveis climáticas e características de crescimento de anéis de árvores de Pinus sibirica nas montanhas de Altai, noroeste da China. Este estudo é o primeiro desse tipo na análise do crescimento climático de Pinus sibirica no noroeste da China. O estudo mostrou grande potencial para entender as espécies que crescem sob as condições climáticas específicas. Total de 70 testemunhos coletados em três locais na área de amostragem, dos quais 63 testemunhos considerados para este estudo. O efeito das variáveis climáticas estudadas incluem precipitação, temperatura e PDSI. Nossos resultados mostraram que a cronologia da Largura do Anel da Árvore tem uma correlação significativamente positiva com a temperatura do final do inverno (março) e uma correlação negativa significativa com as temperaturas de julho. Uma correlação significativa foi observada com a precipitação do final do verão, enquanto nenhuma relação significativa foi encontrada com o Índice de Severidade de Seca de Palmer. Essas correlações significativas com a temperatura e precipitação sugeriram que esta espécie de árvore tinha o potencial para a reconstrução do clima passado na área.

Abstract Climatic factors play an essential role in the growth of tree ring width. In this study, we aimed to evaluate the correlation between climatic variables and tree-ring growth characteristics of Pinus sibirica in Altai mountains, northwestern China. This study being is first of its kind on climate growth analysis of Pinus sibirica in northwestern China. The study showed great potential to understand the species growing under the specific climatic conditions. Total of 70 tree cores collected from three sites in the sampling area, out of which 63 tree cores considered for this study. The effect of climatic variables which was studied include precipitation, temperature and PDSI. Our results showed that Tree Ring Width chronology has a significantly positive correlation with the late winter (March) temperature and significant negative correlation with the July temperatures. A significant correlation was observed with the late summer precipitation whereas no significant relation found with the Palmer Drought Severity Index. These significant correlations with temperature and precipitation suggested that this tree species had the potential for the reconstruction of the past climate in the area.

The goal of this study was to compare the effects of Bacillus subtilis and the antibiotic enrofloxacin on growth performance, immune response, intestinal histomorphometry and cecal Salmonella numbers in broilers challenged with S. gallinarum. Salmonella-free chicks (n = 240) were allocated to four groups, comprising six replicates (n = 10) each. Group NN was not infected and received no treatment. Group SN was infected with Salmonella and received no treatment. Group SE was infected with Salmonella and treated with the antibiotic enrofloxacin. Group SP was infected with Salmonella and treated orally with the probiotic B. subtilis. Initially the groups that were infected with Salmonella had reduced growth performance compared with NN. By the fourth week, SE and SP had recovered and weighed as much as or more than NN. The SP group had greater cellular immunity and relative weights of the bursa and thymus than the other groups. SN had the lowest villus height in all the segments of the small intestine and a larger Salmonella population in the ceca. Probiotic supplementation reduced the pathological outcomes more than enrofloxacin. Prophylactic use of B. subtilis-type probiotics had similar effects to the use of antibiotics, alleviated the stress related to infection of S. gallinarum, and improved the growth performance, immune function, and gut mucosal development in broilers.

Uma nova série de 4-amino-5-aril-3H-1,2,4-triazol-3-tionas, tendo vários grupos metoxibenzila e metoxifenila, foi sintetizada através de refluxo de sais de hidrazina carboditioato de potássio em solução aquosa diluída de hidrato de hidrazina. Estes sais foram formados pela reação de hidrazinas ácidas e dissulfeto de carbono em solução de hidróxido de potássio metanólico a 0-5 ºC. 4-Amino-5-aril-3H-1,2,4-triazol-3-tionas foram condensadas com diferentes ácidos aromáticos substituídos para produzir 1,2,4-triazol[3,4-b]1,3,4-tiodiazols 3,6-disubstituídos. As estruturas dos compostos sintetizados foram caracterizadas pelas espectroscopias no infravermelho (IR) e ressonância magnética nuclear (NMR) de ¹H e 13C, análise elementar e espectrometria de massa (MS). Todos os compostos sintetizados foram examinados com relação as suas atividades de inibição de urease, antioxidante e antibacteriana. Alguns compostos mostraram excelente atividade de inibição de urease, mais do que a droga padrão. Outros compostos apresentaram potente atividade antioxidante. Todos os compostos mostraram atividades antibacterianas significativas em comparação com a droga padrão.

A new series of 4-amino-5-aryl-3H-1,2,4-triazole-3-thiones, bearing various methoxybenzyl- and methoxyphenethyl groups, was synthesized by refluxing potassium hydrazinecarbodithioate salts in dilute aqueous solution of hydrazine hydrate. These salts were formed by the reaction of acid hydrazides and carbon disulfide in methanolic potassium hydroxide solution at 0-5 °C. 4-Amino-5-aryl-3H-1,2,4-triazole-3-thiones were condensed with different substituted aromatic acids to yield 3,6-disubstituted-1,2,4-triazolo[3,4-b]1,3,4-thiadiazoles. The structures of the synthesized compounds were characterized by infrared (IR), ¹H and 13C nuclear magnetic resonance (NMR), elemental analysis and mass spectrometric (MS) studies. All the synthesized compounds were screened for their urease inhibition, antioxidant and antibacterial activities. Some compounds showed excellent urease inhibition activity, more than the standard drug. Others exhibited potent antioxidant activity. All the compounds showed significant antibacterial activities as compared to the standard drug.

Os autores relatam o caso de um jovem de 18 anos de idade com fraqueza muscular generalizada, cãibras e crises convulsivas, tratado com corticosteróides por oito anos em decorrência de um diagnóstico de polimiosite, sem melhora clínica. Ao exame físico apresentava força muscular normal, ausência de atrofias musculares, diminuição de reflexos tendíneos profundos e presença do sinal de Chvostek e Trousseau. A hipótese diagnóstica inicial foi de miopatia metabólica e a investigação laboratorial revelou cálcio sérico e urinário diminuídos e dosagem de paratormônio (PTH) indetectável. O diagnóstico final foi de hipoparatireoidismo, tendo sido o paciente tratado com cálcio endovenoso. A tomografia de crânio demonstrou calcificações difusas, características da síndrome de Fahr. O paciente recebeu alta assintomático com carbonato de cálcio e colecalciferol.

The authors report a case of an 18-year-old boy using prednisone for eight years for a misdiagnosis of polymyositis. His complains were diffuse weakness, cramps and seizures, with progressive worsening and with no improvement despite treatment with corticosteroid. On admission, the physical examination didn't show muscular weakness or atrophy. Deep tendom reflexes were absent and Chvostek and Trousseau signs were found. The diagnosis of a metabolic myopathy was considered and further laboratory findings revealed a low serum and urinary calcium, undetectable PTH. The diagnosis of hypoparathyreoidism was made and the patient was treated with endovenous calcium. Cranial tomography revealed diffuse calcifications, suggestive of Fahr's syndrome. The patient recovered completely and was discharged with oral calcium carbonate and colecalciferol.

O objetivo deste trabalho é analisar a prevalência da disfunção erétil (DE) e a utilidade do Índice Internacional de Função Erétil (IIFE) na investigação da DE em pacientes com esclerose sistêmica (ES). Quinze homens com ES foram questionados acerca dos sintomas da DE e submetidos ao questionário de IIFE. Seus prontuários foram revisados para identificar outras possíveis causas da DE. Os pacientes estudados eram predominantemente caucasóides e tinham ES difusa. DE foi referida por 67% dos pacientes, iniciando-se, em média, 29 meses após o início da ES; quatro pacientes relataram início concomitante da DE e da ES. O diagnóstico da DE pelo IIFE e por interrogação direta foi concordante em todos os pacientes. Não foi encontrada associação nítida entre DE e extensão do envolvimento cutâneo, envolvimento visceral, drogas, escore cutâneo total e duração da doença. Cinco pacientes com DE também tinham outras causas de impotência. A DE afeta um grande porcentual de homens acometidos por esclerose sistêmica e o IIFE pode ser um instrumento válido em sua avaliação. A alta incidência da DE em jovens com ES e a eventual concomitância do início da ES e da DE enfatizam a necessidade de estudos posteriores sobre uma provável relação causal.

The aim of this report is to analyze the incidence of erectile dysfunction (ED) and the usefulness of the International Index of Erectile Function (IIEF) on the investigation of ED in men with systemic sclerosis (SSc). Fifteen male SSc patients were asked about the presence of ED symptoms and were submitted to the IIEF questionnaire, after obtaining informed consent. Their medical data were reviewed in the search of other possible causes of ED. The studied patients were predominantly Caucasian and had diffuse SSc. ED was referred by 67% of them, and it was first noted after an average of 29 months of SSc onset; four patients referred concomitant onset of ED and SSc. All men with ED detected by IIEF questionnaire also reported ED when directly asked, and all men who affirmed having ED were also identified by IIEF. There were no clear association between ED and extent of cutaneous involvement, visceral involvement, drugs, total skin score and disease duration. Five patients with ED also had other causes of impotence. ED affects a high percentage of male SSc patients, and the IIEF may be a valid instrument in its evaluation. The high incidence of ED in young SSc patients and the occasional concomitance of the onset of SSc and ED warrants further evaluation of a causal association.

OBJETIVO: Estabelecer a utilidade da medida indireta da pressão venosa periférica (PVP) na avaliação de pacientes com esclerose sistêmica (ES), comparando com uma população controle normal. MÉTODOS: Estudo prospectivo avaliando 18 pacientes esclerodérmicas do sexo feminino (sendo oito delas com úlcera cutânea isquêmica) submetidas à medida indireta da PVP (por pletismografia). Os dados foram comparados a um grupo controle normal de 18 mulheres hígidas, pareadas para idade. RESULTADOS: Os níveis de pressão arterial foram semelhantes nos dois grupos. Os valores de PVP estavam significativamente diminuídos nos pacientes com ES (58.9 ± 11.6 mmHg no grupo ES e 96.9 ± 7.1 mmHg no grupo controle; p < 0.0001); no grupo com ES, os pacientes com úlceras cutâneas isquêmicas apresentaram níveis de PVP significativamente diminuídos comparando-se com os pacientes com ES sem úlceras (50.6 ± 10.8 mmHg no grupo com úlceras e 65.5 ± 7.2 mmHg no grupo sem úlceras; p = 0.006). CONCLUSÕES: Pode-se inferir que a diminuição nos níveis de PVP na ES parece estar associada a uma diminuição do fluxo sangüíneo, predispondo ao aparecimento de úlceras isquêmicas nestes pacientes.

OBJECTIVE: The aim of this prospective study was to establish the usefulness of indirect measurement of peripheral venous pressure (PVP) in the evaluation of patients with systemic sclerosis (SSc), comparing them with a normal control population. METHODS: Eighteen female SSc patients (eight patients presenting cutaneous ischemic ulcers) were submitted to indirect measurement of the PVP (by plethysmography), and data were compared with a control group of 18 healthy women, paired by age. RESULTS: Arterial pressure levels were similar in both groups. PVP levels were significantly decreased in the SSc patients (58.9 ± 11.6 mmHg in the SSc group and 96.9 ± 7.1 mmHg in the control group; p < 0.0001); in the SSc group, patients with ischemic ulcers presented PVP levels significantly decreased compared with those patients without ulcers (50.6 ± 10.8 mmHg in the ulcer group and 65.5 ± 7.2 mmHg in the group without ulcers; p = 0.006). CONCLUSIONS: It can be concluded that decreased PVP in SSc is associated with a decrease in the blood flow of these patients, predisposing them to cutaneous ischemic ulcers.

A esclerose sistêmica (ES) sine scleroderma é uma doença rara, caracterizada pelo acometimento visceral (geralmente gastrintestinal, pulmonar ou vascular periférico) da ES, na ausência da característica fibrose cutânea. Os autores descrevem um caso da rara ocorrência da crise renal esclerodérmica como manifestação inicial em uma paciente com ES sine scleroderma.

Systemic sclerosis sine scleroderma (ssSSc) is a rare disorder, characterised by visceral involvement (usually peripheral vascular, gastrointestinal and pulmonary) in the absence of the characteristic skin fibrosis. The authors describe in this case report the rare occurrence of sclerodermic renal crisis (SRC) as the initial clinical manifestation in a patient with ssSSc.

Os autores descrevem o caso de uma garota negra, com diagnóstico de síndrome de superposição dermatomiosite e esclerose sistêmica, que desenvolveu calcinose difusa, complicada por infecções secundárias e significativa limitação funcional de membros. Tratamento com colchicina, diltiazem e alendronato sódico não se mostrou eficaz no controle da calcinose, requerendo uso endovenoso bimestral de clodronato, que contribuiu para significativa melhora na cicatrização das úlceras cutâneas e na qualidade de vida.

The authors report an Afro-Brazilian girl with systemic sclerosis and dermatomyositis overlap who evolved with extensive calcinosis, complicated by secondary infections and important disability. Treatment with colchicine, diltiazem and sodium alendronate was not effective in the control of calcinosis, requiring the use of bimonthly intravenous doses of clodronate that resulted in significant improvement in the healing of cutaneous ulcers and in the quality of life of the patient.

OBJETIVO: determinar as manifestações clínicas ao diagnóstico e durante a evolução de pacientes com doença de Still do adulto em um hospital universitário terciário. MÉTODOS: foram identificados 13 pacientes com a doença no Ambulatório de Artrites do HC-UNICAMP, estudados retrospectivamente, com atenção aos sintomas no momento do diagnóstico, à evolução do quadro articular e à medicação utilizada. RESULTADOS: a prevalência da doença foi de 4,3%. A média de idade foi de 30,8 anos com predomínio masculino (54,2%). Todos apresentaram sintomas constitucionais, febre e exantema cutâneo ao diagnóstico. Comprometimento hepático em 100%, com hepatomegalia em 11 (84,65%), esplenomegalia em 4 (30,7%), aumento de enzimas hepáticas em 6 (46,1%) e hipergamaglobulinemia em 10 (76,9%) dos pacientes. Envolvimento cardíaco em 2 (15,4%) e pleurite em 1 (7,7%) paciente. Hematúria foi constatada em 4 (30,7%) casos. A manifestação articular inicial foi predominantemente poliarticular em 8 (66,5%). Todos os pacientes apresentaram leucocitose ao diagnóstico, com predomínio de neutrófilos, ocorrendo linfocitose em 7 (53,8%). Ferritina elevada em 9 (69,2%) pacientes e, após a resolução do quadro, ocorreu normalização em todos. Quanto ao tratamento, todos utilizaram antiinflamatórios não-hormonais e doses baixas de corticosteróides; 7 (69,2%) precisaram usar metotrexato. Doença monocíclica em somente 4 (30,7%) pacientes, predominando doença recorrente. Bloqueio articular em 53,8% dos pacientes, predominantemente em punhos. CONCLUSÕES: embora rara, a doença de Still do adulto deve ser sempre considerada em pacientes com envolvimento sistêmico e poliartrite, após exclusão de outras doenças, principalmente doenças infecciosas e neoplásicas. O curso da doença de Still do adulto é geralmente crônico e a principal seqüela observada neste estudo foi o bloqueio de punhos, em razão de anquilose óssea.

OBJECTIVE: To determine the clinical manifestations at disease onset and during follow-up of patients with Adult-Onset Still Disease (AOSD) in a tertiary university hospital in Brazil. METHODS: Thirteen cases of AOSD were identified in the Arthritis Outpatients Unit - Unicamp. Their clinical records were reviewed retrospectively in order to determine clinical manifestations regarding disease onset, follow-up and prescription. RESULTS: The prevalence of AOSD was 4.3%. The mean age of disease onset was 30.8 years old with a slight prevalence of men (54.2%). Constitutional symptoms, fever and cutaneous rash were observed in all cases. Hepatic involvement was observed in 11 patients, splenomegaly in 4, hematuria in 4, cardiac involvement in 2 and pleuritis in 2. Poliarticular involvement was observed more frequently, and 50% of them presented carpal ankylosis after 3 years of follow-up. Ferritine was elevated in 9 of 13 patients during active disease. All patients used oral non-steroidal anti-inflammatory drugs, steroids and 7 patients needed methotrexate. CONCLUSIONS: Although AOSD is a rare multisystemic rheumatic disorder, it should always be considered in febrile cases with poliarthritis. Neoplasia and infection should always be excluded. Normally it is characterized by a chronic course and carpal ankylosis as the main disabling feature.