ABSTRACT Objective: To evaluate the changes in the work routine of Brazilian pediatric dentists (PD) during the COVID-19 pandemic. Material and Methods: Dentists specializing in pediatric dentistry, working in public or private services, and attending children or adolescents were included. Data collection was performed through a self-administered online questionnaire. Demographic information such as gender, age, work experience time, city size, and place of work was collected; if specific preventive measures were adopted during the pandemic; if there was an increase in service prices and additional costs; and how did the search for urgent or elective dental care take place during 2020 and 2021. A descriptive analysis was performed. Results: A total of 270 PD answered the questionnaire, of which 52.6% interrupted elective care during the pandemic, more than half (51.1%) reduced the number of patients per shift, and more than 70% increased weekly hours. Regarding the financial aspect, 94.7% of participants reported having additional costs, and 51.8% increased the cost of their appointments. In 2020, 72.0% reported a decrease in searches for elective appointments and 58.7% an increase for urgent care. Conclusion: The COVID-19 pandemic affected the search for appointments and the routine of Brazilian PD. For many, the potential financial impact was due to additional costs, periods without patients, and price correction of appointments. Objective (PD COVID19 COVID 19 COVID-1 Methods dentistry services included selfadministered self administered questionnaire gender age time size collected costs 202 2021 Results 27 526 52 6 52.6 51.1% 511 51 1 (51.1% shift 70 hours aspect 947 94 7 94.7 518 8 51.8 720 72 0 72.0 587 58 58.7 Conclusion many COVID1 COVID- 20 2 5 52. 51.1 (51.1 9 94. 51. 72. 58. (51. (51 (5 (

RESUMO Objetivo: avaliar a história de dor e de atendimento odontológico antes e durante a pandemia da COVID-19, assim como a resolutividade do problema odontológico em indivíduos com TEA sob a percepção dos cuidadores. Métodos: Cuidadores de indivíduos com TEA do estado do Rio Grande do Sul responderam a um formulário online. Responderam questões distribuídas em blocos sobre: I) dados de identificação do paciente com TEA e de seu cuidador; II) relato de acesso odontológico antes da pandemia (até março/2020) e III) relato de acesso odontológico durante a pandemia (março/2020 até 2022). Os dados foram tabulados e digitados em uma planilha do Microsoft Excel e analisados por meio de estatística descritiva. Resultados: dos 473 responsáveis por indivíduos com TEA que participavam dos grupos, 72 (15,2%) responderam à pesquisa e foram incluídos neste estudo. Antes da pandemia, 17 indivíduos com TEA haviam sentido dor de origem odontológica (23,7%). Desses, 12 (70,6%) permitiram o atendimento odontológico, segundo o relato dos familiares. Em relação à experiência de dor durante a pandemia, 20 indivíduos (27,8%) sentiram dor, sendo que 17 deles também haviam sentido dor antes da pandemia. Conclusão: a dificuldade de acesso odontológico enfrentada por famílias e indivíduos com TEA é uma realidade, e a persistência da história de dor odontológica foram semelhantes antes e durante a pandemia. Entre aqueles que buscaram atendimento, prevaleceu a procura pelo serviço na rede pública, e a maioria não teve a resolução de seus problemas, destacando a necessidade de melhorias no sistema de saúde bucal para essa população. Objetivo COVID19, COVID19 COVID 19, 19 COVID-19 cuidadores Métodos online sobre I cuidador II março/2020 março2020 março 2020 III (março/202 2022. 2022 . 2022) descritiva Resultados 47 grupos 7 15,2% 152 15 2 (15,2% estudo 1 23,7%. 237 23,7% 23 (23,7%) Desses 70,6% 706 70 6 (70,6% familiares 27,8% 278 27 8 (27,8% Conclusão realidade pública problemas população COVID1 COVID-1 março/202 março202 202 (março/20 4 15,2 (15,2 23,7 (23,7% 70,6 (70,6 27,8 (27,8 COVID- março/20 março20 (março/2 15, (15, 23, (23,7 70, (70, 27, (27, março/2 março2 (março/ (15 (23, (70 (27 março/ (março (1 (23 (7 (2 (

ABSTRACT Objective: Assess the history of pain and dental care before and during the COVID-19 pandemic, as well as the resolution of the dental problems in individuals with ASD from the perspective of caregivers. Methods: Caregivers from three associations of individuals with ASD in the state of Rio Grande do Sul were invited to answer an online questionnaire. They answered questions distributed into three sections: I) identification data of the patient with ASD and his caregiver; II) perception of dental access before the pandemic (until March/2020) and III) perception of dental access during the pandemic (March/2020 until 2022). Data were tabulated into a Microsoft Excel spreadsheet and analyzed using descriptive statistics. Results: Of the 473 caregivers for individuals with ASD in the contacted groups, 72 (15.2%) responded to the survey and were included in this study. Before the COVID-19 pandemic, individuals with ASD had experienced dental pain (23.7%). Twelve of these (70.6%) allowed dental care, according to the caregivers’ reports. Regarding the experience of pain during the pandemic, 20 individuals with ASD (27.8%) felt pain, and 17 of them had also experienced pain before the pandemic. Conclusion: The difficulty of dental access faced by families and individuals with ASD is a reality, and the persistence of dental pain history was similar before and during the COVID-19 pandemic. The preference for services in the public sector prevailed; however, the majority did not find a resolution to their issues, emphasizing the need for improvements in oral health care for this population. Objective COVID19 COVID 19 COVID-1 Methods questionnaire sections I caregiver II March/2020 March2020 March 2020 III (March/202 2022. 2022 . 2022) statistics Results 47 groups 7 15.2% 152 15 2 (15.2% study 23.7%. 237 23.7% 23 (23.7%) 70.6% 706 70 6 (70.6% reports 27.8% 278 27 8 (27.8% 1 Conclusion reality prevailed however issues population COVID1 COVID- March/202 March202 202 (March/20 4 15.2 (15.2 23.7 (23.7% 70.6 (70.6 27.8 (27.8 March/20 March20 (March/2 15. (15. 23. (23.7 70. (70. 27. (27. March/2 March2 (March/ (15 (23. (70 (27 March/ (March (1 (23 (7 (2 (

Abstract The perceptions of parents/caregivers regarding their children’s oral health can influence the standard of care and decision-making regarding oral health. The children’s perspective on their own oral health-related quality of life (OHRQoL) may differ from the parents/caregivers’ views. This cross-sectional study aimed to analyze the agreement between the perceptions of parents/caregivers and schoolchildren regarding OHRQoL. It was conducted with a sample of children between 8 and 11 years old and their parents/caregivers from Pelotas. Children answered the Child Perceptions Questionnaire 8-10 (CPQ), and parents/caregivers answered the short form of the Parental-Caregiver Perceptions Questionnaire (P-CPQ). Socioeconomic data were also collected. Children’s dental caries, traumatic dental injuries, and malocclusions were examined. The Spearman’s correlation test was performed. A total of 119 parents/caregivers-children pairs were included. There was an agreement between the total scores of parents/caregivers and children (0.2770; p = 0.003), in the oral symptoms (0.1907; p = 0.038), and functional limitations (0.2233; p=0.015) domains. The Bland-Altman graph showed an agreement between children’s and parents/caregivers’ OHRQoL total score, but there was an underestimation of approximately two points in the parents/caregivers’ perception compared to the children’s score. There was an agreement between the reports of parents/caregivers and children regarding children’s OHRQoL. parentscaregivers parents caregivers childrens s decisionmaking decision making healthrelated related (OHRQoL views crosssectional cross sectional 1 Pelotas 810 10 8-1 CPQ, CPQ , (CPQ) ParentalCaregiver Parental Caregiver PCPQ. PCPQ P . (P-CPQ) collected Childrens caries injuries examined Spearmans Spearman performed parents/caregiverschildren parentscaregiverschildren included 0.2770 02770 0 2770 (0.2770 0.003, 0003 0.003 003 0.003) 0.1907 01907 1907 (0.1907 0.038, 0038 0.038 038 0.038) 0.2233 02233 2233 (0.2233 p=0.015 p0015 015 domains BlandAltman Bland Altman score 81 8- (CPQ (P-CPQ caregiverschildren 0.277 0277 277 (0.277 000 0.00 00 0.190 0190 190 (0.190 0.03 03 0.223 0223 223 (0.223 p=0.01 p001 01 0.27 027 27 (0.27 0.0 0.19 019 19 (0.19 0.22 022 22 (0.22 p=0.0 p00 0.2 02 2 (0.2 0. 0.1 (0.1 p=0. p0 (0. p=0 (0 p= (

Abstract Caries disease can lead to strong impact in terms of pain and suffering, to functional impairment, and to negative effects on quality of life. Studies have demonstrated that the impact on quality of life increases with dental caries severity, and few studies have assessed the relationship between caries activity and children’s oral health-related quality of life (OHRQoL). This cross-sectional study aimed to assess the impact of dental caries severity and activity on the OHRQoL of schoolchildren. The study was conducted with a sample of children aged 8 to 11 years from Pelotas, southern Brazil. Children answered the Child Perceptions Questionnaire 8–10, and socioeconomic data were collected. Children’s dental caries (Kappa value of 0.95), PUFA, traumatic dental injuries, and malocclusion were examined. The Mann-Whitney, Kruskal-Wallis, and Poisson regression tests were performed. A total of 119 children were included. Children with initial (mean ratio (MR) of 1.92; 95% confidence interval (95% CI) of 1.05–3.48), moderate (MR: 2.66; 95%CI: 1.44–4.90), and severe carious lesions (MR: 2.65; 95%CI: 1.46–4.79) had a greater impact on OHRQoL compared with those without carious lesions (p =0 .047). Children with active carious lesions exhibited a greater impact on OHRQoL (MR:1.53 and 95%CI: 1.11–2.11) when compared to those without active lesions (p = 0.019). The findings demonstrate an association between dental caries severity and activity and the OHRQoL of school-aged children. suffering impairment childrens s healthrelated health related OHRQoL. . (OHRQoL) crosssectional cross sectional schoolchildren 1 Pelotas Brazil 810 10 8–10 collected Childrens Kappa 0.95, 095 0.95 , 0 95 0.95) PUFA injuries examined MannWhitney, MannWhitney Mann Whitney, Whitney Mann-Whitney KruskalWallis, KruskalWallis Kruskal Wallis, Wallis Kruskal-Wallis performed included mean MR (MR 1.92 192 92 (95 CI 1.05–3.48, 105348 1.05–3.48 05 3 48 1.05–3.48) 2.66 266 2 66 95%CI 95CI 1.44–4.90, 144490 1.44–4.90 44 4 90 1.44–4.90) 2.65 265 65 1.46–4.79 146479 46 79 p .047. 047 .047 .047) MR1.53 MR153 1.53 53 (MR:1.5 1.11–2.11 111211 0.019. 0019 0.019 019 0.019) schoolaged school (OHRQoL 81 8–1 09 0.9 9 1.9 19 (9 10534 1.05–3.4 2.6 26 6 14449 1.44–4.9 1.46–4.7 14647 7 04 .04 MR1 MR1.5 MR15 153 1.5 5 (MR:1. 1.11–2.1 11121 001 0.01 01 8– 0. 1. ( 1053 1.05–3. 2. 1444 1.44–4. 1.46–4. 1464 .0 MR1. 15 (MR:1 1.11–2. 1112 00 0.0 105 1.05–3 144 1.44–4 1.46–4 146 1.11–2 111 1.05– 14 1.44– 1.46– 1.11– 1.05 1.44 1.46 1.11 1.0 1.4 1.1

Abstract This study aimed to translate and to perform the cultural adaptation of the instrument Illness Perception Questionnaire-Revised for Dental (IPQ-RD) into Brazilian Portuguese. The IPQ-RD consists of 34 items that assess the cognitive and emotional representation/perception of parents/caregivers of children with dental caries, with response options on a 5-point Likert scale, ranging from “Strongly agree” (score 1) to “Strongly disagree” (score 5). The higher the score, the lower the perception of the disease. The protocol consists of translation into Brazilian Portuguese, back-translation into English, revision by an Expert Review Committee, and pre-test (application in parents/caregivers of children in dental care). For some questions, the translated versions were identical (T1 = T2); for others, one version was preferred (T1 or T2); for still others, it was decided to modify terms to obtain greater clarity on the item (T3). In the first pre-test, three questions were misunderstood by more than 15% of the sample, after which the items were reviewed by the Expert Review Committee. In the second pre-test, the adapted version was applied to a new sample of parents/caregivers (n = 15) and the questions were understood by more than 85% of the participants. The Brazilian Portuguese version of the IPQ-RD was well understood by the evaluated population. QuestionnaireRevised Questionnaire Revised IPQRD IPQ RD (IPQ-RD 3 representationperception representation parentscaregivers parents caregivers caries 5point point 5 scale Strongly agree score 1 disagree 5. . 5) disease backtranslation back English Committee pretest pre test application care. care care) T1 T (T T2 T2) others T3. T3 (T3) pretest, test, 15 n 85 participants population (T3 8

Aim This study aimed to investigate the prevalence of physical violence and head and neck injuries among children and adolescents who suffered physical aggression, firearm aggression, and white gun aggression referred to the Legal Medical Department in a southern city in Brazil. Methods This study was performed at Legal Medical Department in the city of Pelotas, RS, Brazil, from January 2011 to December of 2015. Data were collected from medical reports of children and adolescents (0-19 years old). Demographic information such as sex, age, and skin color was collected. Data were submitted to Chi-square test and multivariate Poisson regression analysis. Results In this study, 2,716 physical examination reports were analyzed, with a total of 2,171 exams resulting from violence; lesions that had physical aggression as their etiology (1,951) had a higher prevalence. The analysis of the exams revealed that the majority were adolescents (90,05%), white (84.09%), and female (50.21%). The head and neck regions were affected in most cases of violence (57.90%). In the adjusted model, the occurrence of injuries in the head and neck region was higher in males (PR 1.16, CI 95% 1.08 - 1.25), among adolescents (PR 1.28, CI 95% 1.10 - 1.48) and in victims of physical aggression compared to victims of the firearm (2.81, CI 95% 1.79 - 4.40). Conclusion The results revealed a high prevalence of head and face injuries in victims of violence and that there was a greater prevalence of physical violence among adolescents compared to children and males. Brazil Pelotas RS 201 2015 019 0 19 (0-1 old. old . old) sex age Chisquare Chi square 2716 2 716 2,71 analyzed 2171 171 2,17 1,951 1951 1 951 (1,951 90,05%, 9005 90,05% , 90 05 (90,05%) 84.09%, 8409 84.09% 84 09 (84.09%) 50.21%. 5021 50.21% 50 21 (50.21%) 57.90%. 5790 57.90% 57 (57.90%) model PR 116 16 1.16 95 108 08 1.0 1.25, 125 1.25 25 1.25) 128 28 1.28 110 10 1.1 1.48 148 48 2.81, 281 81 (2.81 179 79 1.7 4.40. 440 4.40 4 40 4.40) 20 01 (0- 271 71 2,7 217 17 2,1 1,95 195 (1,95 900 90,05 9 (90,05% 840 84.09 8 (84.09% 502 50.21 5 (50.21% 579 57.90 (57.90% 11 1. 12 1.2 1.4 14 2.81 (2.8 7 44 4.4 (0 27 2, 1,9 (1,9 90,0 (90,05 84.0 (84.09 50.2 (50.21 57.9 (57.90 2.8 (2. 4. ( 1, (1, 90, (90,0 84. (84.0 50. (50.2 57. (57.9 2. (2 (1 (90, (84. (50. (57. (90 (84 (50 (57 (9 (8 (5

Aim To verify the validity of maternal reports on the number of deciduous teeth erupted in their children. Methods This cross-sectional study was performed with children enrolled in a Birth Cohort at the age of 12 months in the first stage and 24 months in the second stage. At both stages, children were clinically examined, and mothers reported the number of teeth of their children. Comparison between groups was performed using the Mann-Whitney non-paired Wilcoxon test. Level of agreement between two methods were estimated by the Observed Agreement, Weighted Kappa and Intraclass Correlation coefficients. Results A total of 125 children were examined in the first stage, with mean number of reported teeth of 6.2. In the second stage, 149 children were examined, with mean number of reported teeth of 15.9. High level of agreement, kappa values and intraclass correlation coefficients were observed for both arches in both periods (p<0.001). Conclusions Maternal report on the number of teeth erupted in children was reliable and valid. Thus, it seems to be a useful instrument for collecting data in population-based epidemiological studies targeting young children.

Abstract Objective: To translate and culturally adapt the Modified Child Dental Anxiety Scale - Faces (MCDASf) into Brazilian Portuguese. Material and Methods: The MCDASf consists of eight questions about anxiety toward dental procedures that are answered on a five-point Likert scale ranging from “not worried” (1 point) to “very worried” (5 points). The answers correspond to a scale of face drawings ranging from extremely negative to extremely positive. The process consisted of initial translation, back-translation, expert committee review, and pretesting. Results: The first revised version was applied to 32 children aged 5 to 12 years, 16 from the city of Governador Valadares, Brazil (southeastern region) and 16 from the city of Pelotas, Brazil (southern region). In the first pretest, Item 5 was misunderstood by 6.25% of children in the southern region; Item 6 by 6.25% in both regions; Items 7 and 8 by 87.5% and 100% of those from the southeastern region, respectively, and by 12.5% and 25% of those from the southern region, respectively. Items 7 and 8 were modified by the expert committee, and the second revised version was applied to 32 children, 16 from each region. The only misunderstood items were 4 and 5, both by a 7-year-old boy in Governador Valadares. Conclusion: The Brazilian Portuguese version of the MCDASf was well understood by the sample of children.

RESUMO O objetivo deste trabalho é fornecer embasamento científico para condução do tratamento endodôntico em gestantes através de uma revisão de literatura e série de casos clínicos conduzidos em sessão única no projeto de extensão Atenção Odontológica Materno-infantil da Faculdade de Odontologia da Universidade Federal de Pelotas. As bases científicas abordam as principais dúvidas do profissional na condução do pré-natal odontológico: uso do exame radiográfico, anestésico local, prescrição de medicamentos, entre outros fatores essenciais no atendimento da gestante. As gestantes assistidas procuraram atendimento apresentando dor de origem dentária. Em dois casos foi diagnosticado pulpite irreversível sintomática, enquanto um terceiro se travava de periodontite apical aguda. Os procedimentos endodônticos foram realizados sob condições controladas e padronizadas, sob supervisão de um professor de Endodontia. O preparo dos canais radiculares foi realizado em sessão única, através de instrumentação mecanizada de cinemática reciprocante (Wave One® Gold Primary (Dentsply Sirona), sendo o comprimento dos canais determinado por meio de localizador foraminal FinePex (Schuster). A obturação dos canais foi realizada pela técnica do cone único com cones do sistema Wave One®. Após o tratamento endodôntico foi realizada blindagem coronária com resina composta cor A2 Z350 XT (3M ESPE). Os cuidados relacionados à condição gestacional estão destacados, sendo que as pacientes permanecem em acompanhamento no projeto. Conclui-se que o uso de localizadores foraminais e da instrumentação mecanizada, utilizando limas reciprocantes associadas à técnica de obturação com cone único padronizado para cada sistema representam avanços para a realização do tratamento endodôntico em pacientes gestantes devido ao menor tempo clínico e maior conforto do procedimento.

ABSTRACT The aim of this paper is to provide a scientific basis for conducting safe root canal treatment in pregnant women, illustrating with case reports treated in the extension project Maternal-Child Dental Care at the Federal University of Pelotas. The scientific bases address the main doubts of professionals when performing prenatal dental care: use of radiographic examination, local anesthetics, prescription of medications, among other essential factors in the care of pregnant women. The assisted pregnant women sought care with dental pain. In two cases symptomatic irreversible pulpitis was diagnosed, while a third was experiencing acute apical periodontitis. Endodontic procedures were performed under controlled and standardized conditions. The root canals were prepared in a single session, using mechanical instrumentation with reciprocating kinematics (Wave One® Gold Primary - Dentsply Sirona), and the root canal length was determined by an electronic apex locator FinePex (Schuster). Root canal filling was performed by the single cone technique with Wave One® gutta-percha points. After endodontic treatment, coronal sealing was performed with composite resin A2 Z350 XT (3M ESPE). The care related to the gestational condition is highlighted, and the patients remain under monitoring. It is concluded that the use of apical locators and mechanical instrumentation, using reciprocating files associated with the single cone obturation technique with a standardized cone for each system, represents advances for the accomplishment of endodontic treatment in pregnant patients due to the shorter clinical time and greater comfort of the procedure.

Abstract The purpose of this study was to investigate the association between maternal factors and dental visits of children within their first year of life. This study used data from the 2015 Pelotas Birth Cohort and included pregnant women and their children at 12 months of age, followed-up between May 2014 and December 2015. Socioeconomic and demographic data, information on the use of dental services during the last year, and information on dental fear were collected by interviewing the mothers. Information regarding dental caries and periodontal disease were collected during the mother’s clinical examination. The date of each child’s first visit to the dentist was recorded. Crude and adjusted Poisson regression models with robust variance were used. The sample comprised 2,287 mother-child dyads, of which 10.1% of children visited the dentist during the first year of life. Children whose mothers visited the dentist within the last year for preventive reasons (RR: 1.51; 95%CI: 1.12–2.03) or curative reasons (RR: 1.47; 95%CI: 1.08–2.01) were more likely to have visited the dentist during the first year of life compared to those whose mothers had not used dental services during this period. Maternal tooth loss was inversely associated with a child’s visit to the dentist during the first year of life (RR: 0.69; 95%CI: 0.54–0.89). Considering the mother’s history of using dental services, as well as their dental caries history, is necessary to understand and improve patterns of dental health care utilization and implement public oral health policies for young children.

Abstract: This study aimed to verify, through a longitudinal follow-up, the pre-, peri- and postnatal factors associated with the eruption of deciduous teeth. The study was nested in a Birth Cohort Study conducted in Pelotas, a Southern Brazilian city. Mothers were followed prenatally and their children were followed-up perinatally, at 3 and 12 months of age. The outcome was the number of teeth at 12 months, and exploratory variables included maternal habits and characteristics, anthropometric measures of children and mothers, and socioeconomic and demographic information. Data were collected through interviews with the mother and children’s clinical exam. Poisson regression models were used for the analysis. Participants included 4,014 children with a mean number of erupted teeth at 12 months of 5.50. After adjustments, a lower mean number of teeth was observed in children from non-white mothers, early preterm children, and shorter children at birth and at 12 months. A higher number of teeth was observed for mothers with excessive weight gain during pregnancy, mothers who smoked during pregnancy, weightier children at birth and at 12 months, and for children with larger heads at birth and at 12 months. Our findings suggest that maternal and children characteristics influenced primary tooth eruption.

Abstract This randomized parallel-group control trial tested the efficacy of distraction using audiovisual eyeglasses (AVE) during dental procedures [NCT03902158]. Forty-four 6–9 year-old children with low/moderate anxiety and who needed restorative treatment or exodontia of the primary molars were randomly allocated into two groups: the AVE (experimental) and the conventional behavior management techniques (control) groups. Motion sensors were used to measure the participants’ body movements. Dental visits were video recorded, and their pain levels and behavior were assessed using the Faces, Legs, Activity, Cry, and Consolability Behavioral Pain Assessment Scale and the Venham Behavioral Scale, respectively. Anxiety was assessed via heart rate measurements. After treatment, the children scored their pain using the Faces Pain Scale. Mann-Whitney U and chi-square tests were used to compare the groups. The mean score on the behavioral scale was 0.59 in the experimental group and 0.72 in the control group under local anesthesia (p = 0.73). During the procedure, the mean score was 0.41 in the experimental group and 1.32 in the control group (p = 0.07). The mean heart rate was similar in both groups (p = 0.47), but a significant increase during treatment was observed in the control group. There was no difference between the groups in terms of pain, behavior, and self-reported pain scores (p = 0.08). Children aged 6-7 who used the AVE had fewer wrist movements (435.6) than that of children in the control group (1170.4) (p = 0.04). The AVE achieved similar results to the basic behavior management techniques, with good acceptance by the children.

Abstract This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk. Microcosm biofilms were grown in enamel discs in 24-well plates. Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s. After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed. All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose. Human and bovine milk associated with sucrose showed higher hardness loss. The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05). After 18 hours at rest in pure DMM, an increase in the pH of the supernatant was observed. Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM. Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group. Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.

Abstract This study aimed to evaluate the influence of breastfeeding and pacifier use on the occlusal status of preschool children. A cross-sectional study was conducted with children (n = 489) aged 2–5 years in private and public schools in Pelotas, South Brazil. Mothers answered a questionnaire on demographic, socioeconomic and behavioral variables, including breastfeeding and non-nutritive sucking habits. Malocclusion was classified as normal/mild and moderate/severe, according to WHO criteria. The association between breastfeeding and pacifier use with malocclusion was tested using multivariate logistic regression. The prevalence of malocclusion was 37.83% (95%CI 33.52–42.14), and 57.87% of the children used a pacifier. A total of 46.01% of the sample was exclusively breastfed for 6 months, and 24.74% were never breastfed. Malocclusion was associated with pacifier use, and with both non-exclusively breastfed and never breastfed. After adjustments for demographic and socioeconomic variables, the children who used a pacifier and were never breastfed (OR = 19.08; 95%CI 8.54–42.63), or who used a pacifier and were not exclusively breastfed (OR = 13.74; 95%CI 6.11–30.92), showed worse occlusal conditions, compared with the children who were exclusively breastfed and never used a pacifier. Pacifier use modifies the relationship between breastfeeding and occlusal status.

Resumo Objetivo deste estudo foi determinar a influência de fatores maternos sobre o desenvolvimento de cárie em pré-escolares brasileiros. Este estudo transversal foi aninhado em uma coorte de mães adolescentes. Esta fase foi realizada quando as crianças estavam com idades entre 24 a 42 meses. O questionário aplicado às mães adolescentes incluiu variáveis demográficas, socioeconômicas e a escolaridade materna. Os exames clínicos foram realizados nas mães e em seus filhos. O exame clínico das mães avaliou dentes cariados, perdidos e restaurados na dentição permanente (CPOD) e condição gengival; o exame clínico dos filhos avaliou dentes cariados, perdidos e restaurados na dentição decídua (ceo-d). Regressão de Poisson com variância robusta foi utilizada para estimar a razão de prevalência, risco relativo e os intervalos de confiança de 95%. Os dados também foram utilizados para identificar os fatores de risco maternos associados com o desfecho (prevalência e severidade da cárie na infância). No total, foram avaliados 538 díades mãe-criança; a prevalência de cárie precoce na infância foi de 15,1% e a cárie materna ocorreu em 74,4%. Após ajuste, as crianças que apresentaram uma maior incidência de cárie dentária eram filhas de mães de baixo nível socioeconômico, que apresentam mais dentes cariados e mais sangramento gengival. Os resultados deste estudo sugerem que a saúde bucal das mães é um fator de risco potencialmente importante para o desenvolvimento da cárie da primeira infância. Gestores de saúde pública devem considerar esta informação ao planejar intervenções, a fim de prevenir a ocorrência de cárie da primeira infância.

Abstract This study aimed to determine the influence of maternal factors on the early development of dental caries in Brazilian preschoolers. This cross-sectional study was nested in a cohort of adolescent mothers. The current wave was performed when the children were aged 24 to 42 months. The questionnaire-based survey targeted adolescent mothers and included demographic and socioeconomic variables as well as the maternal education level. In addition, clinical examinations were performed on the mothers and their children. Mothers were assessed for decayed, missing and filled teeth in the permanent dentition (DMFT index) and gingival assessment; their children were assessed for decayed, missing and filled teeth in the deciduous dentition (dmft index). Poisson regression with robust variance was used to estimate the prevalence ratio, risk ratio and 95% confidence intervals. This data was also used to identify the maternal risk factors associated with the outcomes (prevalence and severity of childhood caries). A total 538 mother-child dyads were evaluated; the prevalence of early childhood caries was 15.1% and maternal caries was 74.4%. After the adjustment, the children that exhibited a greater incidence of dental caries were from mothers of low socioeconomic status, or from those presenting decayed teeth and higher rates of gingival bleeding. The results of this study suggest that the oral health of mothers is a potentially important risk factor for the development of early childhood dental caries. Public health planners should consider this information when planning interventions in order to prevent the occurrence of early dental caries.