Reducing non-specific adsorption of proteins on the surface of magnetic nanoparticles (MNPs) is becoming increasingly important. In this paper, we proposed a novel surface modification procedure by grafting hyperbranched polyglycerol (HPG) onto the surface of MNPs (Fe3O4@SiO2@MAA), in which lots of hydroxyl groups from HPG not only provide the hydrates sheath to prevent non-specific adsorption of proteins, but also react with succinic anhydride to generate carboxyl groups that serve as active sites to specifically bind proteins. The protein adsorption experiments showed that the non-specific adsorption (0.07 μg mg-1) was reduced to 4.58% of that before modification. It also showed that the antigen binding capacity was 9.7 times higher than the original when detecting cardiac troponin I (cTnI) in human plasma samples, which indicated that the final synthesized MNPs had great application prospects in bio-separation and bioanalysis. nonspecific non specific (MNPs important paper (HPG Fe3O4@SiO2@MAA, Fe3O4SiO2MAA FeOSiOMAA Fe3O4@SiO2@MAA , Fe3O4 SiO2 MAA Fe O SiO (Fe3O4@SiO2@MAA) 0.07 007 0 07 (0.0 mg1 mg 1 mg-1 458 4 58 4.58 97 9 7 9. cTnI (cTnI samples bioseparation bio separation bioanalysis FeO Fe3O (Fe3O4@SiO2@MAA 0.0 00 (0. mg- 45 5 4.5 0. (0 4. (

ABSTRACT Due to its high nutritional value, broccoli (Brassica oleracea var. italica Plenck) is one of the most popular vegetables worldwide. This study assessed 36 phenotypic characteristics of 111 broccoli varieties to understand the phenotypic diversity of new broccoli varieties and improve their breeding speed with advantages and characteristics in China, including 108 new varieties and three varieties of common knowledge. The genetic diversity, the principal component, and the cluster of phenotypic characteristics of broccoli varieties were further investigated. The results showed that the coefficients of variation of 36 characteristics ranged between 11.18 % and 94.99 %, with their diversity index between 0.26 and 1.82. The 111 broccoli varieties were further classified into eight groups, primarily attributed to the differences in phenotypic characteristics, including curd weight, main stem thickness, plant development degree, plant height, and anthocyanin coloration. The cumulative contribution rate of the first five principal components reached 81.186 %, corresponding to 12 representative phenotypic traits. The analysis indicated that the phenotypic characteristics of broccoli were rich in diversity, especially for several characteristics appreciated by the market, such as weight, curd firmness, and anthocyanin coloration. This study revealed the basic information on the genetic diversity of new broccoli varieties in China from 2017 to 2019 and provided potential breeding strategies for broccoli to meet diverse market demands. value Brassica var Plenck worldwide 3 11 10 knowledge component investigated 1118 18 11.1 9499 94 99 94.9 026 0 26 0.2 182 1 82 1.82 groups weight thickness degree height coloration 81186 81 186 81.18 traits firmness 201 demands 11. 949 9 94. 02 2 0. 8 1.8 8118 81.1 20 1. 811 81.

The fluorescent microspheres are prepared by the swelling-evaporation method. The optimal swelling conditions are obtained by researching related factors, such as types and dosages of swelling agent, dosages of fluorescent substance, swelling time and swelling temperature. Considering that the structure of microspheres can influence the swelling properties, the paper mainly focuses on the effect of molecular weight and surface carboxyl group content of polystyrene microspheres on swelling properties. When the molecular weight of microspheres is 75276 g mol-1, the density of carboxyl group on their surface is 6.94×10-4 mol g-1, and the doping amount of europium complex reaches the maximum of 24.9 mg g-1 with the strongest fluorescence intensity. Meanwhile, the as-prepared fluorescent microspheres have high monodispersity, uniform particles size and high thermal stability (the decomposition temperature is 385oC), which sets up the good foundation for biomolecule detection. So, this work provides a significant experience for the preparation of fluorescent microspheres.

PURPOSE: To systematically evaluate the clinical efficacy of reamed and nonreamed intramedullary nailing in treatment of closed tibial fractures with Cochrane systematic review methods. METHODS: According to the Cochrane systematic review methods, literatures were retrieved from Cochrane library, PubMed, EMbase and other database. Randomized controlled trials (RCTs) and quasi-randomized controlled clinical trials about reamed and nonreamed intramedullary nailing in the treatment of closed tibial fractures were collected and RevMan 5.0 was chosen for meta-analysis. RESULTS: A total of seven studies were included in this meta-analysis. Reamed intramedullary nailing was better than nonreamed intramedullary nailing in nonunion rate [P = 0.02, RR = 0.46, 95% CI: (0.24, 0.91)] and implant failure rate [P <0.0001, RR = 0.36, 95% CI: (0.22, 0.57)]. No statistically significant difference was observed in malunion rate, compartment syndrome rate, postoperative infection [P = 0.18, RR = 0.50, 95% CI: (0.18, 1.383); P = 0 43, RR = 0.77, 95% CI: (0.40, 1.48); P = 0.27, RR = 0.38, 95% CI: (0.01, 7.87)]. CONCLUSION: Compared with the nonreamed intramedullary nailing, reamed intramedullary nailing can lead to better outcome in the treatment of closed tibial fractures.

The prosthetic leg (PL) is a typical human-machine system in which the dynamic interaction between the human body and PL (machine) determines a high requirement of ergonomics design for PL and consequently needs to consider an indicator of usability indicating the performance of gait biomechanics. How to evaluate the indexes of usability for PL products is critical to the design technology of PL based on ergonomics. The gait symmetry of PL products, which is a core usability index, was experimentally analyzed by using a specially designed testing device in this paper. The test results show that the swing speed symmetry for intelligent prosthetic leg (IPL) is high up to 96.5%, which indicates a high performance for gait tracking. Then, a comprehensive evaluation was made for the usability of PL products with the analysis method of Grey Correlation Degree. Three types of PL product, including ordinary PL, IPL and Gait-following IPL (GL-IPL), were used in the evaluation. The evaluation results show that the GL-IPL product is the best in usability while both of IPL and GF-IPL products based on ergonomics are obviously better than the ordinary PL. The method of usability evaluation studied here is expected to help directing the design of prosthetic leg products.