ABSTRACT The aim of this study was to assess the sperm quality and testicular histomorphometry of Wistar rats supplemented with extract and fractions of fruits of Tribulus terrestris L. The ethanolic extract was obtained by dynamic maceration of spray-dried fruit. This extract was fractionated by liquid-liquid partition, using increasing polarity solvents. Twenty male rats were separated in four groups, with five rats in each group. The control was supplemented with distilled water, while the others were daily given the ethanolic extract, hexanic or aqueous fraction soluble in methanol in a dose of 42 mg.kg-1.day-1 for 70 days. Sperm was obtained from the right epididymal tail for the analysis of motility, count, morphology and viability. The testicular weight of groups supplemented with ethanolic extract and aqueous fraction soluble in methanol was higher when compared to the control. The gonadosomatic index increased in the group supplemented with ethanolic extract. The nuclear, cytoplasmic and individual volume of Leydig cells increased in supplementation with hexanic and aqueous fractions soluble in methanol. It was concluded that the extract influenced the spermatogenesis, while hexanic and aqueous fractions soluble in methanol promoted the changes in the intertubular compartment. Therefore, Tribulus terrestris did not improve the sperm quality of the rats.

The aim of this study was to evaluate the therapeutic and toxicologic effects of the administration of the powered vegetable extract and aqueous extract of Tournefortia paniculata leaves on Wistar rats, subjected to a hypercaloric diet for 42 days. The rats were divided into five groups and were given the following treatments by gavage: T0 (control) - 1.0 mL water day-1; T1 - aqueous extract containing 14 mg phenolic compounds kg-1 rat day-1; T2 - 14 mg quercetin kg-1 rat day-1; T3 - 50 mg powered vegetable extract from T. paniculata leaves (PVE) kg-1 rat day-1 and T4 - 100 mg PVE kg-1 rat day-1. The treatments did not significantly alter the weight, but were effective in reducing liver fat, glucose and serum triglycerides. The treatment T1 reduced food consumption and lipid peroxidation. None of the treatments showed genotoxic potential. Results showed that T. paniculata leaves possessed an anti-obesity potential. However, a more detailed study of the medicinal potential and characterization of phytochemicals in this plant would be still necessary for a better understanding of its mechanisms of action, enabling future applications in the treatment of this pathology or for various therapeutic purposes.

This study aimed at evaluating the effect of ractopamine (RAC) on metabolism, zootechnical performance, body composition, and behavior in Wistar rats submitted to acute and chronic restrain stress. The oral dose of 5 mg/kg of RAC was administered in periods of 0, 7, 14, 21, and 28 days. The elevated plus-maze test (EPMT) was used for behavioral assessment. Blood, carcass and viscera characteristics were evaluated. Insulin-dependent glucose transporters (GLUT-4) were semi-quantified by Western Blot in epididymal adipocytes. RAC periods associated with chronic stress increased the GLUT-4 protein expression in adipose tissue in a time-dependent manner (P=0.01), i.e., the longer the RAC addition period, the higher the GLUT-4 concentration in chronically stressed animals (0=1.42; 7=1.19; 14=2.03; 21=1.59; 28=2.35). The stress periods combined with RAC increased the time spent in the opened arms of the maze (Chronic stress: 0=10.6; 7=8.7; 14=5.9; 21=12.3; 28=4.0; Acute stress 0=3.1; 7= 4.7; 14=7.5; 21=0.0; 28=2.8) (P=0.04). Chronic (entries on the closed arms [ECA]=3.60) and acute (ECA=3.80) stress reduced locomotive activity in the maze (P=0.03). The results suggested that stress could negatively affect the possible benefits offered by the RAC, mainly impairing the adipose tissue metabolism and behavior in the animals.

Inúmeras pesquisas buscam fármacos com propriedades cicatrizantes, capazes de reparar lesões cutâneas. O objetivo deste estudo foi caracterizar quimicamente uma quitosana e avaliar seus efeitos cicatrizantes em lesões cutâneas de ratos. A quitosana foi caracterizada quanto à viscosidade, massa molar, grau de acetilação, análise elementar, termogravimetria e espectroscopia na região do infravermelho. As propriedades cicatrizantes da quitosana foram avaliadas, para isso ratos adultos foram submetidos à excisão tecidual e tratados por 21 dias, sendo avaliados parâmetros macroscópicos, semanalmente, e microscópicos, ao final do experimento. A quitosana apresentou viscosidade de 5 mPa s, baixo grau de acetilação (GA=5,9%) e massa molar (132,0 kDa). Por análise elementar observou-se que a composição da quitosana se aproxima da fórmula C6H11O4N, suas principais bandas características foram observadas no espectro de infravermelho e a curva termogravimétrica mostrou que esta apresenta somente duas etapas de decomposição. Não houve diferença significativa na histologia, no 21º dia da lesão, entre os grupos controle e tratado, porém, macroscopicamente, a quitosana promoveu redução de 81,4% da largura das lesões, porcentagem significativamente superior à observada no grupo controle (71,2%), auxiliando a cura de lesões cutâneas, o que reforça seu potencial de aplicação médica.

Several researches look for drugs with healing properties, capable to cure skin lesions. The objective of this study was to chemically characterize one chitosan and evaluate its healing effects in mice skin lesions. The chitosan was characterized in relation to the viscosity, molar mass, acetylation degree, elementary analysis, thermogravimetry and infrared spectroscopy. To evaluate the healing properties of chitosan mices were submitted to tissue excision and treated during 21 days. The macroscopic parameters were weekly evaluated and the microscopic parameters at the end of the experiment. Chitosan presented viscosity of 5 mPa s, low acetylation degree (GA= 5.9%) and molar mass of 132.0 kDa. By elemental analysis, we observed that the composition of chitosan is similar to the formula C6H11O4N, its main characteristic bands were observed in the infrared spectrum and the thermogravimetric curve showed two decomposition steps. There was no significant difference on the histology between the control and the treated group after the 21st day of lesion. Macroscopically, chitosan caused the reduction of 81.4% of the lesions width, which is significantly superior to the observed reduction in the control group (71.2%), thus helping the cure of skin lesions. These results reinforces its potential use for medical application.

Introduction: Functional foods have been widely utilized to reduce the symptoms of various diseases such as diabetes mellitus (DM). Among the foods used to combat these effects are soluble fibres, mainly those rich in beta-glucans (BGs). Objective: To review the effects of beta-glucans (BGs) on glucose plasmatic levels of diabetic individuals. Design: A search was conducted using the Pubmed, Science Direct and Scielo databases using the keywords: diabetes mellitus and beta-glucan and glucose and glycaemia. As inclusion criteria, only studies on diabetic human individuals (type 1 or type 2) who consumed BGs were selected. Results and Discussion: Of the 819 initial articles retrieved, only 10 fit the inclusion criteria and were used in the study. It was observed that doses around 6.0g/person/ day, for at least 4 weeks were sufficient to provoke improvements in the blood glucose levels and also lipid parameters of individuals with DM. However, glucose levels do not reach normal levels using BG alone. Low doses of BG for at least 12 weeks were also reported to promote metabolic benefits. Conclusions: Based on previous research, it was concluded that the ingestion of BGs was efficient in decreasing glucose levels of diabetic patients. The consumption of greater doses or smaller doses for longer periods of time produced better results.

Introducción: Alimentos funcionales han sido ampliamente utilizados para reducir los síntomas de diversas enfermedades como la diabetes mellitus (DM). Entre los alimentos utilizados en el combate de estos efectos, están las fibras solubles, principalmente aquellas que tienen buena cantidad de beta-glucano (BG’s). Objetivo: El objetivo de esta revisión sistemática fue evaluar los efectos de los BG´s en los parámetros metabólicos de individuos diabéticos. Métodos: Fue conducida una búsqueda en las bases de datos Pubmed, ScienceDirect y cielo, utilizando las siguientes palabras-clave: diabetes mellitus and beta- glucano and glucosa and glucemia. Como criterio de inclusión, fueron seleccionados solamente estudios en individuos diabéticos (tipo 1 o tipo 2) que consumieron BG´s. Resultados y Discusión: De los 819 trabajos inicialmente encontrados, 10 artículos se encuadraron en los criterios de inclusión, y por eso fueran utilizados en el estudio. Fue observado que dosis superiores de 6,0 g/ individuo/día, o dosis más grandes que 3,0 g/individuo/ día por un periodo de tiempo más largo, son suficientes para provocar mejoras en los parámetros glucémicos y lipidicos de portadores de DM. Bajas dosis de BG por al menos 12 semanas también presentaron efectos metabólicos benéficos. Conclusión: Tomando en cuenta los resultados observados, se concluye que los BG´s son eficientes en la atenuación de los efectos indeseables del DM, siendo las dosis más grandes o el consumo de pequeñas cantidades por un tiempo más largo las que promueven resultados mejores.

This study evaluated the effect of ractopamine (RAC) on the performance of finishing pigs and the meat quality of these animals. Seventy crossbred pigs (35 barrows and 35 females) selected for high gain of lean meat, with initial weight of 77.1±0.32 kg were distributed in randomized blocks with five treatments (0, 5, 10, 15, and 20 ppm RAC in the diet) and seven replications during 28 days. The experimental unit was represented by a male and a female pig. Regarding the performance variables, there was a linear increase in final weight with increasing levels of RAC, as well as in average daily weight gain. An improvement in feed conversion was observed for animals fed RAC, and the optimal level - estimated by the LRP model - was ~ 5 ppm. For feed intake, no significant effect on intake of digestible lysine and energy intake was observed. Carcass yield responses increased linearly with the RAC dose. Ash content, color component b* and loss drip linearly decreased with increasing doses of RAC. There was also a significant difference in the percentage of ether extract and crude protein in the loin, and treatment with 20 ppm RAC showed a lower amount of protein and larger amounts of lipids. Moisture content, color component L*, weight loss by cooking and defrosting, shear force and pH were not affected by the treatment. Concerning the lipid oxidation, there was no effect of RAC on the concentration of TBARS (thiobarbituric acid reactive substances) under cooling and under freezing. Thus, all ractopamine levels improve performance compared with control and do not negatively affect the quality of fresh, chilled or frozen pig meat.

The aim of this article was to evaluate the in vivo mechanism of action through which ractopamine reduces the amount of adipose tissue in carcass of animals fed diets supplemented with this drug. Literature search was carried out, using the keywords Ractopamine, lipogenesis, lipolysis, fatty acid and adipose tissue. The scoring system was designed for the adequacy of the methodology. A total of eight papers were found - all published in the English language. The determination of lipolysis and lipogenesis was carried out using different methods - from the quantification of non-esterified fatty acids in the blood to molecular analyses of adipose tissue. The review analysis suggested that, at least in the pigs, the use of this drug reduced lipid deposition in the carcass due to a greater inhibition of lipogenesis than an increase in lipolysis.

Performance and carcass and cut yield of finishing pigs of three sexual categories fed diets with different levels of ractopamine were studied. Forty-eight hybrid animals (initial weight of 92.1±2.4 kg) were distributed in a completely randomised design in 3 × 2 factorial arrangement (three sexual categories: surgically castrated males, two females and immunologically castrated males - with or without 10 ppm of ractopamine), totalling six treatments and eight repetitions with one animal per experimental plot during 28 days. Ractopamine improved weight gain and feed conversion of all sexual categories. The best results were obtained with immunologically castrated males, which, in turn, had lower carcass yield and backfat thickness in the belly. In the absence of ractopamine, depth and loin eye area were higher in females but no differences in these variables were observed between categories when this additive was used. Ractopamine increased the sirloin yield in all sexual categories. There was no difference in bonus index. Ractopamine improves the performance of pigs of 92 to 125 kg. Immunologically castrated males have the best performance responses to ractopamine, but with losses in carcass yield, loin characteristics and backfat thickness of the belly.

The objective of this study was to find an alternative to minimize environmental contamination by leather waste using it as ruminant feed. The wet blue leather wastes (WB) without chrome extraction were compared with the leather wastes in which the chrome was extracted (CE). Both materials had 99.7% of dry matter (DM), but the crude protein level was higher (90.4%) in CE than in WB (74.3%). In situ effective ruminal degradability of DM was 59.7% and it was 63.1% for CP in CE. The WB did not suffer degradation in the rumen. In vitro abomasal digestibility of CE was 100%. The percentage of degradation per hour was higher for CE (8.2%) than for WB (0.08%). The mineral content was higher in wet blue leather wastes (10.4%) than in CE (0.4%) reflecting the chrome level and demonstrating that the removal process of this mineral is efficient. The use in animal feed is presented as a viable alternative for the disposal of waste and scrap generated by the leather tanning industry and treated by the extraction method, thus minimizing environmental contamination and providing a source of protein for animal feed.

O polvilho da fruta-de-lobo é um produto extraído da polpa da fruta-de-lobo verde (Solanum lycocarpum St. Hil), popularmente utilizado. Pouco se conhece a respeito desse polvilho, apesar de ele ser comercializado em farmácias na região de Lavras-MG, na forma de cápsulas de 300 mg. É utilizado no tratamento da diabetes e a ele são atribuídos outros efeitos terapêuticos, como a redução da obesidade e do colesterol, mas sem conhecimento científico e sem conhecer sua composição centesimal. Com o objetivo de conhecer sua composição centesimal, foram coletadas 80 frutas-de-lobo na área de pastagem do Departamento de Zootecnia, na Universidade Federal de Lavras. Foram selecionadas 61 frutas, levando em consideração o tamanho e o estádio de maturação. As frutas selecionadas foram lavadas, descascadas e picadas, as sementes retiradas, trituradas e o polvilho obtido foi filtrado, decantado, lavado com água destilada por duas vezes e seco em estufa de ventilação. A amostra foi triturada em um gral de porcelana e armazenada em um recipiente de vidro hermeticamente fechado e protegido da luz, sob temperatura ambiente. Foram realizadas as análises de composição centesimal, observando-se que, no polvilho da fruta-de-lobo, os carboidratos são os constituintes predominantes (84,99%), seguidos de fibras solúveis (1,35%), fibras insolúveis (1,60%), proteínas (0,60%), lipídeos (0,07%), cinzas (0,03%) e umidade (11,16%). Os teores de fibras alimentares encontrados no polvilho da fruta-de-lobo podem estar relacionados aos efeitos terapêuticos que são atribuídos a ele.

Fruit-of-wolf flour is a product extracted from the unripe fruit-of-wolf pulp (Solanum lycocarpum St. Hil), according to folk knowledge. Little is known of that starch; in spite of it is being marketed in pharmacies in the region of Lavras, in 300 mg capsules. It is utilized in the treatment of diabetes and it is also ascribed other therapeutic effects such as reduction of obesity and cholesterol, but without any scientific knowledge and without knowing its centesimal composition. With the objective of knowing its centesimal composition, eighty fruits were collected in the pasture area of the Animal Science Department of the UFLA/Lavras-MG. Sixty-one fruits were selected, taking into account the size and maturation stage. The selected fruits were washed, peeled, chopped, the seeds removed and the obtained starch was filtered, decanted, washed with distilled water twice and dried in an air forced oven. The sample was ground in a china mortar and stored in air-tightly sealed glass container and protected from light at room temperature. The centesimal composition analyses were performed and observing that in the fruit-of-wolf flour, carbohydrates are the predominant constituents (84.99%), followed by soluble fibers (1.35%), insoluble fibers (1.60%), proteins (0.60%), lipids (0.07%), ashes (0.03%) and moisture (11.16%). The contents of food fibers found in the fruit-of-wolf flour can be related to the therapeutic effects which are ascribed to it.

O objetivo deste trabalho foi determinar a atividade antioxidante do café, bebida mole, in vivo e in vitro, antes e após a torração. Para a análise da atividade antioxidante in vitro, foram utilizados os métodos de sequestro de radicais livres (DPPH) e de atividade quelante de íons Fe2+. Foram utilizados, para o ensaio in vivo, ratos Zucker diabéticos, portadores de síndrome metabólica, e ratos Zucker controle. Os animais receberam doses diárias das bebidas de café, por gavagem, por 30 dias. Após o tratamento, foi realizada a avaliação de peroxidação lipídica. As amostras torradas apresentaram a maior percentagem de sequestro de radicais livres. As concentrações nas amostras de café verde e torrado foram similares às do padrão Trolox. Das amostras torradas, a torração média se destacou com maior atividade quelante de íons Fe2+. Os cafés verdes mostraram maior poder quelante do que os torrados. Compostos presentes no extrato diminuíram a lipoperoxidação hepática e renal que é comum em casos de diabetes e síndrome metabólica. O café apresenta atividade antioxidante e protege o fígado e os rins dos animais contra a lipoperoxidação comumente presente em quadros de diabetes mellitus tipo 2 e síndrome metabólica.

The objective of this work was to determine the in vivo and in vitro antioxidant activity of soft coffee beverages, before and after roasting. For the analysis of the antioxidant activity, the methods of free radical sequestration (DPPH) and of the chelating activity of Fe2+ ions were used. In vivo tests were performed with diabetic Zucker rats with metabolic syndrome and control Zucker rats. The animals received daily doses of coffee drinks by gavage for 30 days. After treatment, lipid peroxidation was evaluated. Roasted coffee samples had the highest percentage of free radical sequestration. Concentration in both green and roasted coffee samples was similar to that of the Trolox standard. From the roasted samples, medium roast stood out with greater Fe2+ ion chelating activity. Green coffees showed higher quelating power than the roasted ones. The compounds in the extract decreased the lipid peroxidation in the liver and kidney which is common in cases of diabetes and metabolic syndrome. Coffee has an antioxidant activity and protects the liver and kidneys of animals from lipid peroxidation commonly present in type 2 diabetes mellitus and metabolic syndrome.

Conduziu-se o presente estudo, com o objetivo de avaliar o efeito de diferentes tipos de fibras sobre as fraçoes lipídicas do sangue e fígado de ratos. Para determinar o efeito da pectina citrica, goma guar e celulose sobre o perfil lipidico dos ratos utilizaramse 36 ratos machos da linhagem wistar. Os ratos foram distribuidos aleatoreamente em seis grupos. Cada gruopo foi submetido a uma dieta por 58 dias. As dietas utilizadas foram: A- 10% de pectina, B - 15% de pectina, C - 10% de goma guar, D- 15% de goma guar, E - 5% de Celulose sem colesterol e F - 5% celulose com 1% de colesterol. A pectina e a goma guar evitaram o aumento do colesterol total e LDL-c sérico observado na dieta F (p<0,05). Não houve diferença significativa entre os tipos de fibras e a quantidades utilizadas. Os níveis séricos HDL-c, VLDL-c e TAG permaneceram inalterados em todos os tratamentos. Os resultados mostram o potencial efeito benéfico da ingestão das fibras solúveis pectina e goma guar no controle da colesterolemia.

The present study was designed to evaluate the effect of different types of fibers on lipid portions of mice blood and liver. To determine the effect of citric pectin, guar gum and cellulose on the rat lipid profile, 36 male mice of the Wistar strain were utilized. The mice were allocated randomly into six groups. Each group was submitted to an experimental diet for 58 days. The utilized diets were: A- 10% pectin, B - 15% pectin, C - 10% guar gum, D- 15% guar gum, E - 5% cellulose with no cholesterol and F - 5% cellulose with 1% cholesterol. Pectin and guar gum prevented the increase of total cholesterol and serum LDL-c found in diet F (p<0.05). There was no significant difference among the types of soluble fibers and the amounts utilized. The serum levels HDL-c, VLDL-c, and TAG did not alter in all the treatments. The results show the potential beneficial effect of the ingestion of the soluble fibers pectin and guar gum in cholesterolemia control.

Folhas de mandioca possuem substâncias como ligninas e saponinas que podem apresentar efeito hipolipidêmico. Todavia, um estudo recente relatou aumento no peso do fígado de ratos alimentados com dietas contendo farinha de folhas de mandioca (FFM - Manihot esculenta Crantz cv. Cacao), tornando-se necessário um estudo mais aprofundado dos efeitos desta farinha sobre os parâmetros hepáticos. Para este estudo, um ensaio biológico com 32 ratos machos Wistar foi conduzido por um período de 7 semanas, sendo os tratamentos: dieta controle e dietas contendo 5, 10 e 15% de FFM. As dietas contendo FFM não apresentaram efeitos sobre as atividades das enzimas Aspartato Aminotransferase (AST) e Fosfatase Alcalina (FA), mas aumentaram significativamente a atividade da enzima alanina aminotransferase (ALT). O estudo histopatológico revelou vacuolização do citoplasma dos hepatócitos para todos os grupos. No entanto, a freqüência de animais com vacuolização acentuada foi superior nos grupos que receberam dietas com FFM, apresentando também maiores teores de lipídios e colesterol total hepáticos e maior relação peso fígado/peso corporal. Estes resultados indicam que os antinutrientes presentes nas folhas de mandioca, como taninos, cianeto e saponinas, podem ser responsáveis pela redução da função hepática nos animais alimentados com FFM.

Cassava leaves contain substances such as lignins and saponins that can present the hypolipidemic effect. However, a recent study has reported an increase in liver weight of rats fed diet containing cassava leaves flour (CLF - Manihot esculenta Crantz cv. Cacao). Thus, a further study of the effect of this flour on the hepatic parameters is necessary. For the development of this study, a biological assay with 32 male Wistar rats was conducted for a period of 7 weeks with the following treatments: control diet and diets containing 5, 10, and 15% of CLF. The diets containing CLF showed no effects on the Aspartate Aminotransferase (AST) and Alkaline Phosphatase (ALP) enzymes activity, but they significantly increased the Alanine Aminotransferase (ALT) enzyme activity. The histopathologic study showed hepatocytes cytoplasm vacuolization for all groups. However, the number of animals with sharp vacuolization was higher in the groups that received diets with CLF , which also showed higher levels of hepatic lipids, total cholesterol, and greater ratio liver weight / body weight. These results indicate that the antinutrients in the cassava leaves, such as tannins, cyanide, and saponins are probably responsible for the reduction in the hepatic function of animals fed with CLF.

Um dos componentes da multimistura para suplementação alimentar de populações carentes é a farinha de folhas de mandioca (FFM) que possui elevado conteúdo em proteínas, vitaminas e minerais. Todavia, as folhas de mandioca também apresentam substâncias antinutritivas e/ou tóxicas, como cianeto, polifenóis, nitrato, ácido oxálico, hemaglutinina, saponinas e inibidores de tripsina. Objetivou-se neste trabalho extrair as proteínas da FFM, purificando-as em coluna cromatográfica e determinar sua atividade hemaglutinante e toxicidade. Foram testadas várias estratégias de extração e precipitação das proteínas, sendo que o maior teor protéico e atividade hemaglutinante foi obtido na extração com água destilada na proporção 1:20 (p/v) seguida da precipitação com sulfato de amônio a 80% de saturação. As proteínas precipitadas foram purificadas em coluna Q-Sepharose. Das quatro frações obtidas na purificação (I, II, III e IV), a I e a II apresentaram maiores atividades hemaglutinantes. As mesmas frações foram injetadas via intraperitoneal em camundongos com doses de 2mg (fração I), 3mg (fração II), 54mg (fração III) e 52mg (fração IV) para cada animal com 20g de peso médio, não sendo observadas mortes ou quaisquer efeitos adversos após 120h.

One of the components of the multimixture to the feed supplementation of low-income populations is cassava leaf flour (FFM), with high content of proteins, vitamins and minerals. However, cassava leaves also present substance regarded as antinutritive and/or toxic, such as cyanide, polyphenols, nitrate, oxalic acid, hemagglutinin, saponins and trypsin inhibitors. The aim of this work was to extract proteins from FFM, purifying them in chromatographic column and determine their hemagglutinating activity and toxicity. A number of strategies of extraction and precipitation of proteins were tested; the highest protein content and hemagglutinating activity were obtained in the extraction with distilled water at the 1:20 ratio (p/v) followed by the precipitation with ammonium sulfate at 80% of saturation. The precipitated proteins were purified in Q-Sepharose Fast Flow column. Out of the four purification fractions (I, II, III e IV), the I and II activities presented higher specific activity. The same fractions were injected intraperitoneal via in mice of 20g weight with doses of 2mg (fraction I), 3mg (fraction II), 54mg (fraction III) and 52mg (fraction IV). No deaths or any adverse effects was observed after 120h.

Um experimento foi conduzido para avaliar o efeito da suplementação do complexo enzimático Endopower <FONT FACE=Symbol>bÒ</FONT>, composto de a-galactosidase, galactomanase, xilanase e beta-glucanase e da forma física da ração sobre o desempenho e as características de carcaça de frangos de corte. Foram utilizados 1488 frangos de corte machos, distribuídos num delineamento inteiramente casualizado, oito tratamentos e seis repetições. Os tratamentos constituíram-se de rações à base de milho e de farelo de soja, sem adição de enzimas ou com 200 ou 400 g de enzimas por tonelada de ração e duas formas físicas (farelada e peletizada). Dois tratamentos adicionais continham dieta basal farelada com energias do milho e farelo de soja valorados em 2 e 9%, respectivamente e digestibilidade de aminoácidos valorados em 4% para ambos ingredientes, sendo um tratamento sem adição de enzimas e outro com a adição de 200 g por tonelada de ração. O desempenho foi avaliado de 1 a 21 dias e 1 a 42 dias de idade das aves. Houve efeito da suplementação enzimática no consumo de ração, nos dois períodos avaliados (P<0,05), o qual reduziu linearmente. A conversão alimentar melhorou (P<0,05) no período de 1-21 dias. O teor de gordura abdominal aumentou linearmente (P<0,05). Houve interação entre suplementação enzimática e forma física da ração na conversão alimentar (P<0,01) e ganho de peso (P<0,05) no período de 1-42 dias, sendo a forma peletizada sem enzimas a que proporcionou melhor desempenho (P<0,05). O ganho de peso de 1-21 dias e o rendimento de carcaça foram superiores nas aves recebendo ração peletizada (P<0,05). A valoração do milho e do farelo de soja não afetou o desempenho (P>0,05) dos frangos de corte 1-21 dias de idade.

A study was conducted in order to verify the effect of enzyme supplementation (Endopower<FONT FACE=Symbol>bÒ</FONT>, based on de alpha-galactosidase, galactomanase, xilanase e beta-glucanase) and feeding different physical forms of diets on performance and carcass yield of broilers from 1 to 42 dais old. A total of 1488 broilers chickens, 8 treatments, 6 replicates and 31 broilers per experimental unit plot were fed with diets based on corn and soybean, with enzyme supplementation 200 or 400g for ton. and without enzymes supplementation and two physical forms (meal and pelleted ). Two additional basal diets with the energetic values of corn and soybean meal valued in 2 and 9% respectively and digestibilidade of amino acids digestibility valued in 4% by using one treatment with no enzyme supplementation and another one with enzyme supplementation of 200g/ton. Two periods 1-21 and 22-42 days old were evaluated. Reduced feed intake were observed in linear way (P<0.05) by the enzyme supplementation (P<0.05) in two periods. Broilers fed diets supplemented showed better feed conversion in the period of 1-21 days (P<0.05). Percentage of abdominal fat increased linearly with the increase of the supplementation level of enzyme (P<0.05). The interaction between enzyme supplementation and physical form was significant (P<0.05) for weight gain (P<0.05) and feed conversion (P<0.01) in the period of 1-42 days old . Pelleted diets showed better weight gain in the 1- 21 days (P<0.05). The pelleted diet improved performance to the broilers fed diets without enzyme supplementation (P<0.05). The carcass yield and weight gain in the period of 121 days were better in broilers fed pelleted diets (P<0.05). ). The valued energetic data of corn and soybean meal shown any difference (P>0.05) on the broiler performance from 1 to 21 days old.