Através de estudo do tipo transversal com amostra constituída por 386 crianças e adolescentes atendidos em um ambulatório especializado de filariose, do Recife, nordeste do Brasil, determinou-se a frequência de anticorpos anti-Toxocara e sua relação com faixa etária, sexo, número de eosinófilos periféricos, microfilárias de Wuchereria bancrofti e parasitos intestinais. A freqüência encontrada de anticorpos IgG total anti-Toxocara, realizada através da técnica de ELISA, foi de 39,4%, com 40,1% no sexo masculino e 37,6% no feminino, diferença esta sem significância estatística. O grupo com maior freqüência de anticorpos anti-Toxocara foi o de 6-10 anos (60%) e, apenas nessa faixa etária, encontrou-se uma diferença estatisticamente significante quanto ao sexo, com predomínio do masculino. Observou-se associação estatisticamente significante entre o número de eosinófilos e a presença de anticorpos anti-Toxocara. A freqüência de parasitos intestinais foi de 52,1%, porém sem associação entre este achado e a presença de anticorpos anti-Toxocara. Na presente análise, 42,2% dos pacientes eram portadores de microfilárias de Wuchereria bancrofti, porém esta infecção não esteve associada à presença de anticorpos anti-Toxocara o que sugere que não houve cruzamento do ELISA com a presença de parasitoses intestinais e filariose.

In a transversal study on a sample of 386 children and adolescents from an outpatient clinic for filariasis in Recife, Northeast Brazil, the frequency of anti-Toxocara antibodies and its relation to age, gender, number of peripheral eosinophils, Wuchereria bancrofti microfilariae and intestinal helminths was determined. The total anti-Toxocara IgG antibody frequency was 39.4%, by ELISA technique. The difference in frequency between males (40.1%) and females (37.6%) was not statistically significant. The 6 to 10-year-old subset presented the highest frequency of anti-Toxocara antibodies (60%), and within this age group there was a statistically significant male bias. There was also a significant association between the number of eosinophils and the presence of anti-Toxocara antibodies. Intestinal parasite frequency was 52.1%, but no association was found between this data and the presence of anti-Toxocara antibodies. In the present sample, 42.2% of the patients were Wuchereria bancrofti carriers, however, again this was not associated with the presence of anti-Toxocara antibodies. In conclusion, anti-Toxocara antibodies were highly prevalent in this sample. The present data show that there is no cross correlation between anti-Toxocara IgG antibody and the presence of intestinal helminths and filariasis.

Immunodetection of human IgG anti-Toxocara canis was developed based on ELISA and on the use of polysiloxane/polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. Using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates. However, the difference between positive and negative sera mean absorbances was larger for this new glass based assay. In addition to the performance of the POS/PVA bead as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application.

A group of youngsters (4-18 years old) in northeast Brazil was studied to establish the prevalence of anaemia and intestinal parasitism, as well as to analyze the correlation between them. Two criteria were used to determine the state of anaemia, the level of haemoglobin and the mean of corpuscular volume. The first was considered a single criterion and the second an associated criteria, used in an attempt to correlate anaemia with iron deficiency. The prevalence of intestinal parasitism was 93%, while the prevalence of anaemia was 43.1% and 16.1% according to the criteria employed (single or associated respectively). Anaemia was significantly associated with both sex and age. No significant statistical difference was observed when the association was made between each parasite and anaemia even with those more related to anaemia.