ABSTRACT This work evaluated the initial performance and genetic diversity of Coffea canephora genotypes cultivated in environments at contrasting altitudes. Fourteen morphophysiological traits and seven descriptors of the genus Coffea spp. of coffee trees cultivated at altitudes of 140 m and 700 m were evaluated. The design used was Federer’s augmented block in a 2 × 112 factorial scheme with six blocks. The first factor was the two environments, and the second was the 112 genotypes, with eight common treatments, being five conilon coffee clones and three arabica coffee cultivars. The data were analyzed by the method of REML/BLUP and genetic correlation method. Genetic diversity was evaluated by estimating the distance matrix, applying the Gower methodology followed by the clustering method by Tocher and UPGMA. The phenotypic means were higher in the environment at an altitude of 700 m, except for plant height, number of leaves, and canopy height (CH). Genotypic effects were significant for most traits except for leaf width, CH, unit leaf area, and total leaf area. A wide genetic diversity was verified, with distances varying from 0.037 to 0.593 for the pairs of genotypes 26 × 93 and T7 × 76, respectively. Most of the traits studied showed high genotypic correlation with the environment and expressive genetic correlation between the evaluated traits thereby demonstrating the possibility of indirect selection. There is an adaptation of conilon coffee genotypes to high altitudes and the possibility of developing a specific cultivar for the southern state of Espírito Santo. spp 14 70 Federers Federer s 11 blocks treatments cultivars REMLBLUP REML BLUP matrix UPGMA leaves CH. CH . (CH) width area verified 0037 0 037 0.03 0593 593 0.59 9 T 76 respectively selection Santo 1 7 (CH 003 03 0.0 059 59 0.5 00 0. 05 5

ABSTRACT. Coffee outturn can be defined as the ratio between the harvested coffee and its respective processed grains. This character is greatly influenced by genotypic and environmental effects, and in breeding programs your analysis is costly and time-consuming. In this sense, the use of an outturn index to estimate coffee yield on experimental plots is a desirable measure aiming at reducing resources and time in postharvest evaluations. Thus, the present study aimed to evaluate the accuracy of the use of an outturn index equal to 4.0, in the estimation of Conilon coffee grains production. This index indicates that four kilograms of harvested fruit would be needed to obtain one kilogram of processed grains. Based on the average of 157 genotypes conducted in three trials and four harvests, we evaluated the relationship between harvested fruits and processed grains (FcBe), the observed (OGY), and the estimated grain yield per plant (EGY) based on FcBe equal to 4.0 (an outturn index). Descriptive statistics, adequation test for EGY, and the coincidence of occurrence of genotypes observations relating to the top 20% of all observations of OGY and EGY. In the estimation of grain yield in Conilon, the use of FcBe equal to 4.0 showed high precision in the average of the analyzed trials. However, further studies should be conducted to elucidate the effects of climate variables on the yield of Conilon coffee, especially in atypical crop years. Thus, the use of an outturn index becomes interesting in cases where the number of genotypes to be evaluated is very large and a screening of the promising ones is desirable.

Abstract: The state of Espírito Santo is the major producer of Coffea canephora in Brazil. Knowledge of genetic reserves is fundamental to plant breeding. Therefore, the present study characterized and analyzed the genetic diversity of 600 C. canephora accessions from the germplasm bank of Incaper based on 38 traits evaluated in 24-30-month-old plants. Further, the predominant descriptors or traits were identified, and high phenotypic variability was determined. Genetic distances for the grouped (Gower), quantitative, and qualitative datasets were 0.48, 0.61, and 0.92, respectively, with accessions 76 (Conilon) and 407 (Robusta) being the most divergent ones at Incaper. In clustering using the Tocher optimization method, 30 groups were formed, with three accessions introduced from Epamig’s Robusta collection being the most dissimilar ones. Graphical dispersion analysis using the principal coordinate method revealed the predominance of three groups formed by the Robusta, Conilon, and hybrid Robusta × Conilon genotypes.

RESUMO O objetivo deste trabalho foi avaliar o comportamento de 54 clones de cafeeiro conilon oriundos do Programa de Melhoramento Genético do Incaper à ferrugem em condições controladas de temperatura e fotoperíodo. Utilizou-se o delineamento inteiramente casualizado com três repetições, sendo cada repetição composta por 16 discos de folha cada, acondicionados em gerbox e inoculados com 104 esporos.mL-1 de H. vastatrix. Os gerbox foram colocados sob ausência de luz e 22ºC por 48 horas e então em fotoperíodo de 12 horas até o término do experimento. Foram avaliados os componentes de resistência: período de incubação, período latente, incidência, porcentagem de discos com esporulação, número de esporos e severidade. Com base nos resultados verificou-se a formação de três grupos de genótipos de café conilon: Resistentes, Moderadamente Resistentes e Suscetíveis. No grupo Resistente foram alocados 19 genótipos, no grupo Moderadamente Resistente foram alocados 19 genótipos e no grupo Suscetível foram alocados 16 genótipos. Assim, com base nos resultados, conclui-se que há variação no nível de resistência dos genótipos de C. canephora à H. vastatrix. Tal informação subsidia os programas de melhoramento na seleção adequada de progênies de C. canephora quanto à resistência à ferrugem.

ABSTRACT The objective of this study was to evaluate the behavior of 54 conilon coffee clones from the Incaper Genetic Improvement Program to rust under controlled conditions of temperature and photoperiod. A completely randomized design with three replicates was used, and each replicate was composed of 16 leaf disks each, which were packed in gerboxes and inoculated with 104 spores.mL-1 H. vastatrix. The gerboxes were stored in the absence of light at 22ºC for 48 hours and subsequently at 12-hour photoperiod until the end of the experiment. The evaluated resistance components were: incubation period, latent period, incidence, percentage of disks with sporulation, number of spores and severity. Based on the results, three groups of conilon coffee genotypes were identified: Resistant, Moderately Resistant and Susceptible. To the Resistant group, 19 genotypes were allocated; to the Moderately Resistant group, 19 genotypes were allocated and to the Susceptible group 16 susceptible genotypes were allocated. Thus, results led to the conclusion that there is a variation in the resistance level of C. canephora genotypes to H. vastatrix. Such information subsidizes breeding programs in properly selecting C. canephora progenies for resistance to rust.

Resumo: O objetivo deste trabalho foi apresentar uma descrição sensorial de bebidas de café conilon (Coffea canefora) produzidas a partir de genótipos desenvolvidos para o Estado do Espírito Santo. Foram avaliados os genótipos 'Diamante ES8112', 'ES8122' e 'Centenária ES8132'. Nove amostras oriundas de três clones (genótipos) de cada cultivar, procedentes de duas regiões distintas do estado, foram analisadas. Uma equipe de testadores para a avaliação sensorial de café foi treinada para a obtenção do perfil descritivo e a seleção de termos. As bebidas de café foram descritas como apresentando uma maior intensidade dos atributos fragrância do pó de café, aroma característico da bebida, corpo, sabor característico e sabor residual, e uma menor intensidade de amargor, acidez, adstringência e defeitos. Os termos amargor, forte, encorpado, adstringência e sabor residual característico de café foram os mais relevantes na caracterização das bebidas das cultivares de C. canephora estudadas. A acidez é o atributo com maior impacto sobre a qualidade global da bebida de C. canephora. As bebidas dos clones das cultivares 'Diamante', 'ES8122' e 'Centenária ES8132' classificam-se como na faixa de qualidade tradicional, porém próxima à categoria superior.

Abstract: The objective of this work was to present a sensory description of conilon coffee (Coffea canephora) brews produced from genotypes developed for the state of Espírito Santo, Brazil. The genotypes 'Diamante ES8112', 'ES8122', and 'Centenária ES8132' were evaluated. Nine samples from three clones (genotypes) of each cultivar, from two different regions of the state, were analyzed. A panel of tasters for coffee sensory evaluation was trained to obtain a descriptive profile and to select terms. Beverages were described as showing a higher intensity of powder fragrance, characteristic aroma of coffee brew, body, characteristic flavor and aftertaste, and a lower intensity of bitterness, acidity, astringency, and defects. The terms bitterness, strong, body, astringency, and coffee aftertaste were the most relevant in the characterization of the beverages of the studied C. canephora cultivars. Acidity is the attribute with the greatest impact on the overall quality of C. canephora brew. The coffee brews of the clones of 'Diamante ES8112', 'ES8122', and 'Centenária ES8132' are classified as of traditional quality, but near the superior category.

Abstract The objective of this study was to describe the genetic parameters, adaptability, and stability of clones from the Coffea canephora variety ‘Vitória Incaper 8142’, and the genetic divergence between them. Following 12 and 24 months of plant growth, several traits were evaluated, including stem diameter, plant height, canopy diameter, number of plagiotropic branches, and number of nodes per plagiotropic branch. The agronomic performance of four crops was determined using measurements of productivity, yield, percentage of floating grains, and grade of the beans. Variability was observed between the clones for the majority of the traits measured. The clones V7, V10, V12, and V13 were the most stable and displayed the highest degree of adaptation for the studied traits, and thus these clones will be used to establish a base population suited to the growing conditions in Alto Suaçuí, Minas Gerais, Brazil. Additionally, the genetic dissimilarity identified between clones allowed suitable clone combinations to be proposed for use in future crosses.

The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or Robusta), III = one progeny of un-defined C. canephora (Conilon or Robusta) and IV = one progeny of C. canephora of Robusta group. The grouping formed was consistent with the origins of each group. High stabilities of the bifurcations were found by bootstrap analysis. The use of molecular markers of the SSR and ISSR types in the diversity study was efficient in distinguishing genotypes between and within C. arabica and C. canephora.

Objetivou-se avaliar o crescimento de mudas propagadas por enxertia e por estaquia no cafeeiro Conilon. O experimento foi conduzido no viveiro de produção de mudas da Fazenda Experimental do Incaper, no município de Marilândia, Estado do Espírito Santo. Na enxertia utilizou-se como porta-enxerto, mudas provenientes de propagação seminífera, da espécie Coffea canephora, cv. Robusta Tropical (ENCAPER 8151), e como enxerto, seis clones do cv. Conilon Vitória (INCAPER 8142). A estaquia foi realizada com seis clones usados na enxertia. O delineamento experimental foi em blocos casualizados com doze tratamento e cinco repetições composta de doze mudas. Aos 150 dias avaliou-se as médias das variáveis, que foram comparadas pelo teste de Scheffé a 5% de probabilidade. Detectou-se superioridade para as mudas enxertadas em quase todas as características avaliadas, sendo, portanto mais uma possibilidade para a propagação do cafeeiro conilon.

The purpose of this study was to assess the growth of conilon coffee tree plantlets that were propagated by grafting and cutting. The experiment was conducted at the plantlet production site of Incaper's Experimental Farm in the city of Marilândia, Espírito Santo State. For grafting, plantlets derived from the seed propagation of Coffea canephora cv. Robusta Tropical (ENCAPER 8151) were used as rootstocks, and six clones of cv. Conilon Vitória (INCAPER 8142) were used as the grafts. The cutting was performed with six clones that were used for grafting. The experimental design consisted of randomized blocks of twelve treatments with five repetitions composed of twelve plantlets. On the hundred and fifth day, the averages of the variables were assessed and compared by the Scheffé test at a probability of 5%. The grafted plantlets were superior for almost all of the characteristics assessed, which suggests that it is possible to propagate conilon coffee trees.

O objetivo deste trabalho foi determinar alterações fisiológicas e de tolerância à seca em clones de café Conilon (Coffea canephora) contrastantes quanto à sensibilidade ao deficit hídrico. Foram avaliadas as enxertias recíprocas entre os clones 109A, sensível ao deficit hídrico, e 120, tolerante - 120/109A, 120/120, 109A/120, 109A/109A -, além de seus respectivos pés-francos. As plantas foram cultivadas em vasos de 12 L em casa de vegetação. Após seis meses, metade das plantas foi submetida ao deficit hídrico por meio da suspensão da irrigação, até que as folhas atingissem o potencial hídrico de antemanhã de -3,0 MPa. Quando o clone 120 foi usado como porta-enxerto, as plantas apresentaram sistema radicular mais profundo, mas com menor massa, retardaram por mais tempo a desidratação celular das folhas e apresentaram maior eficiência no uso da água. Sob seca severa, os teores de amido e sacarose decresceram em todos os tratamentos, enquanto os teores de glicose, frutose, aminoácidos totais e prolina aumentaram, particularmente nos tratamentos 109A pé-franco, 109A/109A e 120/109A. Essas plantas apresentaram menor eficiência no uso da água. O acúmulo de solutos não foi associado à tolerância à seca. O uso de porta-enxertos tolerantes à seca contribui para a maior tolerância das plantas ao deficit hídrico.

The objective of this work was to determine alterations in physiology and those due to drought tolerance on Conilon coffee (Coffea canephora) contrasting clones regarding the sensitivity to hydric stress. The reciprocal grafting between clones 109A, drought sensitive, and 120, drought tolerant, - 120/109A, 120/120, 109A/120, 109A/109A - along with their ungrafted control plants (109A and 120) were evaluated. Plants were cultivated in 12-L vases in greenhouse. Six months after grafting, half of the plants was subjected to water deficit, by suspending irrigation until leaves reached the hydric potential of -3,0 MPa. When clone 120 was used as rootstock, plants presented deeper roots, although with lower root-biomass, higher ability to postpone leaf dehydration and higher instantaneous water-use efficiency (WUE). Under severe drought, starch and sucrose contents decreased similarly, regardless of the treatment, whereas leaf concentrations of glucose, fructose, total amino acids and proline were higher in non-grafted 109A, 109A/109A, and 120/109A plants. These plants showed the lowest WUE values. Solute accumulation was not primarily related to drought tolerance. The use of drought tolerant rootstocks improves to drought tolerance in coffee.

O objetivo deste trabalho foi avaliar uma metodologia de análise de adaptabilidade e estabilidade fenotípica de genótipos de café baseada em regressão não paramétrica. A técnica utilizada difere das demais, pois reduz a influência na estimação do parâmetro de adaptabilidade de algum ponto extremo, ocasionado pela presença de genótipos com respostas demasiadamente diferenciadas a determinado ambiente. Foram utilizados dados provenientes de um experimento sobre produtividade média de grãos de 40 genótipos de café (Coffea canephora), com delineamento em blocos ao acaso, com seis repetições. Os genótipos foram avaliados em cinco anos (1996, 1998, 1999, 2000 e 2001), em dois locais (Sooretama e Marilândia, ES) no total de dez ambientes. A metodologia proposta demonstrou ser adequada e eficiente, pois extingue os efeitos impróprios induzidos pela presença de pontos extremos e evita a recomendação incorreta de genótipos quanto à adaptabilidade.

The objective of this work was to evaluate a methodology of phenotypic adaptability and stability analyses of coffee genotypes based on nonparametric regression. The technique used differs from other techniques because it reduces the influence of extreme points resulting from the presence of genotypes whose answers to a certain environment are too different on the estimation of the adaptability parameter. Data from an experiment studying the average yield of 40 coffee (Coffea canephora) genotypes in a randomized block design with six replicates were used to evaluate the method. The genotypes were evaluated along five years (1996, 1998, 1999, 2000 and 2001) in two locations (Sooretama and Marilândia, ES, Brazil), in a total of ten environments. The methodology proposed proved adequate and efficient, since it eliminates the disproportionate effects induced by the presence of extreme points and avoids misleading recommendations of genotypes in terms of adaptability.

O objetivo deste trabalho foi avaliar a produtividade e obter as estimativas de parâmetros genéticos e não genéticos de 40 materiais genéticos do programa de melhoramento genético de café Conilon do Incaper, no Estado do Espírito Santo. Foram analisados dados de dois experimentos, nos municípios de Marilândia e Sooretama, ES, nas safras de 1996, 1997, 1998, 1999, 2000, 2001 e 2002, em que se avaliaram 16 características. Realizou-se, inicialmente, a análise de variância individual, por local em cada ano, com base na média de parcelas, em blocos ao acaso. Posteriormente foi feita a análise de variância conjunta. Os genótipos apresentaram grande variabilidade genética para a maioria das características avaliadas. Os elevados coeficientes de determinação genotípico e coeficientes de variação genéticos, associados às altas produtividades e à variabilidade genética indicam a possibilidade de obtenção de êxitos em programas de melhoramento genético para diferentes características avaliadas nos dois municípios.

The objective of this work was to evaluate the performance and to obtain the estimates of the genetic and nongenetic parameters in 40 genetic materials of the Conilon coffee breeding program conducted by Incaper, Espírito Santo State, Brazil. Two experiments were conducted in Marilândia and Sooretama counties, ES, in 1996, 1997, 1998, 1999, 2000, 2001 and 2002 crop seasons, and 16 characteristics were evaluated. Initially, the individual variance analysis was yearly accomplished for each locality, based on the average of the plots in a randomized block experimental design. Later, the combined variance analysis was performed. The genotypes showed high genetic variability on most evaluated traits. The high genotypic determination coefficients and the genetic variation coefficients associated to the high productivities and genetic variability indicate the possibility to obtain success in the genetic breeding programs for the different characteristics evaluated in both counties.

O objetivo deste trabalho foi avaliar a divergência genética entre 32 clones de café conilon (Coffea canephora Pierre ex Frohener) componentes de três variedades clonais melhoradas, com vistas à identificação dos mais dissimilares, para o estabelecimento de programas de cruzamentos dirigidos. A divergência genética foi avaliada por procedimentos multivariados: distância generalizada de Mahalanobis, método de agrupamento de otimização de Tocher e técnica de variáveis canônicas. Sete caracteres foram avaliados em experimento conduzido em Marilândia, ES. Os genótipos ES 92, ES 25 e ES 22 são os mais divergentes, sendo os dois últimos os mais indicados para cruzamento com os demais, tendo em vista aliarem divergência genética a um bom desempenho produtivo.

The objective of this work was to evaluate the genetic divergence between 32 clones of three clonal varieties of conilon coffee, to identify the most dissimilar for the establishment of programs of directed crosses. Genetic divergence was evaluated by multivariate procedures: generalized Mahalanobis distance, the grouping optimization method of Tocher and the technique of canonical variables. Seven characteristics were evaluated in one trial conducted in Marilândia, ES, Brazil. Genotypes ES 92, ES 25 and ES 22 are the most divergent; the last two are the most appropriate for crossing and to obtain hybrids of improved performance by using genetic divergence for good productive performance.

O objetivo deste trabalho foi avaliar a possibilidade do emprego de índices de seleção em complexos fatoriais, de modo a auxiliar na seleção simultânea de caracteres de Coffea canephora var. Conilon, na predição de ganhos por seleção. Foram analisados e avaliados quatorze características e 40 genótipos de dois experimentos, pertencentes ao programa de melhoramento genético de café 'Conilon' do Incaper, Marilândia, ES, e em Sooretama, ES. Os experimentos foram instalados em delineamento de blocos ao acaso, com quatro repetições e duas plantas úteis por parcela. O uso dos complexos fatoriais eliminou o problema da multicolinearidade e permitiu a adequada utilização da teoria de índice de seleção, para melhoramento simultâneo de caracteres. Foram utilizados os índices de seleção de Smith e Hazel e de Pesek & Baker, e o primeiro foi a melhor alternativa na obtenção de ganhos simultâneos adequados. A utilização conjunta das técnicas multivariadas de análise de fatores, para simplificação estrutural prévia no número de caracteres, e de índices de seleção na predição de ganhos simultâneos são alternativas eficientes no melhoramento genético da cultura.

The objective of this work was to evaluate the possibility of using selection indexes in factorial complexes, in the simultaneous selection of characters of Coffea canephora (Conilon var.), when predicting the selection gains. Fourteen characters and 40 genotypes were analyzed in two experiments of the genetic improvement program of 'Conilon' coffee, developed by Incaper, carried out in Marilândia and Sooretama, ES, Brazil. The experiments were conducted on a randomized block design, with four replicates and two useful plants in each plot. Factorial complexes eliminated the multicolinearity problem, allowing the appropriate use of the selection index theory for simultaneous improvements of characters. The selection indexes by Smith and Hazel and Pesek & Baker were used, and the first one was the best alternative to obtain adequate simultaneous gains. The combined use of multivariate techniques of the factor analysis, for previous structural simplification in the number of characters, and selection indexes to predict simultaneous gains are efficient alternatives in the coffee cropping genetic improvement.

O objetivo deste trabalho foi avaliar a divergência genética de genitores de feijão tolerantes e não-tolerantes às condições de inverno e de suas combinações híbridas. A distância generalizada de Mahalanobis, o método de agrupamento de otimização de Tocher e a técnica de variáveis canônicas foram os procedimentos multivariados utilizados. Nos cruzamentos, utilizaram-se cultivares de feijão que se adaptam bem às condições de inverno, ou seja: Vermelho 2157, Ouro Negro, Antióquia 8 e Ricopardo 896, e as cultivares comerciais não-tolerantes, EMCAPA 404 -- Serrano, Carioca e EMCAPA 405 -- Goytacazes. Os genitores e as combinações híbridas nas gerações F1, F2 e F3 foram avaliados em Coimbra, Minas Gerais, em quatro ensaios, nos anos de 1995 e 1996. A divergência genética dos germoplasmas foi influenciada pela temperatura e pelo estádio de melhoramento. As cultivares mais dissimilares foram Antióquia 8 e EMCAPA 404 -- Serrano, e as mais similares foram, Ouro Negro e Ricopardo 896. O rendimento de grãos e o número de vagens por parcela apresentaram-se como as características de menor importância relativa no estudo da divergência genética. No entanto, como apresentaram baixa correlação genotípica com as demais características e eram as de maior importância no processo produtivo, não devem ser descartadas.

This work aimed to evaluate the genetic divergency among tolerants and non tolerants parents to cold and their hybrid combinations. The generalized distance of Mahalanobis, the method of cluster analysis proposed by Tocher and the canonical variability technic were the multivaried procedures used. Common bean cultivars of two different groups were used in the crosses, being Vermelho 2157, Ouro Negro, Antióquia 8 and Ricopardo 896 the cultivars tolerant to cold, and the commercial cultivars EMCAPA 404 -- Serrano, Carioca and EMCAPA 405 -- Goytacazes the non tolerant ones. The parents and the hybrid combinations in the F1, F2 and F3 generations were evaluated in Coimbra, Minas Gerais on four experiments, in 1995 and 1996. The genetic divergency among the genetic resources evaluated were influenced by temperature and advancement of the generations. The most dissimilar cultivars were Antióquia 8 and EMCAPA 404 -- Serrano and Ouro Negro and Ricopardo 896 were the most similar cultivars. Grain yield and number of pods per plot were the less important characteristics influencing on genetic divergency. However, as they showed low genotypic correlation with the other characteristics and were of the greatest importance on yield, they can not be despised.