ABSTRACT Zinnia sp. and hairy beggartick (Bidens pilosa) plants exhibiting symptoms of possible virus infection were found in the municipality of Santa Bárbara d’Oeste, São Paulo State, Brazil. Flexuous filamentous particles and cytoplasmatic inclusions typical of potyvirus infection were observed by transmission electron microscopy, respectively, in leaf extracts and cells of symptomatic leaves. Infection of both plants with bidens mottle virus (BiMoV) was confirmed by RT-PCR using potyvirus universal primers, followed by nucleotide sequencing of the amplicons. The nearly complete genome sequence of the Brazilian isolate, named BiMoV-BR, is 9700 nucleotides long and shares 95.6 % identity with the corresponding nucleotide sequence of a BiMoV isolate from the United States. BiMoV-BR was mechanically transmitted and caused systemic infection on plants of Zinnia sp., hairy beggarstick, sunflower (Helianthus annuus), and lettuce (Lactuca sativa). Myzus persicae transmitted the virus to Zinnia sp. plants with efficacy of 8 % and 42 %, using one and ten aphids per plant, respectively. This is the first detection of BiMoV in Brazil. Further studies are necessary to evaluate the distribution of this potyvirus in the country. sp Bidens pilosa dOeste, dOeste d Oeste, Oeste d’Oeste State Brazil microscopy respectively leaves (BiMoV RTPCR RT PCR primers amplicons BiMoVBR, BiMoVBR BR, BR 970 956 95 6 95. States beggarstick Helianthus annuus, annuus , annuus) Lactuca sativa. sativa . sativa) 4 plant country 97 9

ABSTRACT Sunflower (Helianthus annuus) plants showing symptoms of chlorosis, mosaic, chlorotic ringspot, and necrosis on younger leaves were found in a small experimental plot in Piracicaba, in the state of São Paulo, Brazil. Preliminary examinations by transmission electron microscopy of symptomatic leaf tissue revealed flexuous filamentous particles 13-15 nm wide and 700-750 nm long, and cytoplasmatic cylindrical inclusions typical of those found in plant cells infected by members of the Potyvirus genus. Total RNA extracted from symptomatic leaves and subjected to RT-PCR followed by partial nucleotide sequencing confirmed the presence of a potyvirus in the affected plants, which was identified as sunflower chlorotic mottle virus (SuCMoV), a member of the Sunflower chlorotic mottle virus (genus Potyvirus, family Potyviridae) species. Mechanical transmission assays with extracts of symptomatic sunflower leaves reproduced the original symptoms in sunflowers, mosaic symptoms in Zinnia elegans, and chlorotic local lesions in Chenopodium amaranticolor and C. quinoa. Sunflower and zinnia plants became infected after aphid transmission experiments with Myzus persicae. RT-PCR tests using specific primers for SuCMoV confirmed the presence of this virus in experimentally infected plants, meeting the criteria of Koch’s postulate. This is the first report of SuCMoV infecting sunflower plants in Brazil.

ABSTRACT: This work is the first record of Encarsia inaron (Walker, 1839) (Hymenoptera: Aphelinidae) parasitizing Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) on soybeans in Brazil. Morphological and molecular characterizations are provided, in addition to verification of the presence of E. inaron in areas infested by whiteflies, indicating that the wasp can potentially be used in integrated management programs. Additionally to morphological verification, the molecular analysis allowed the identification of some degree of genetic variability in the genome of the parasitoid.

RESUMEN: Este es el primer registro de Encarsia inaron (Walker, 1839) (Hymenoptera: Aphelinidae) parasitando Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) en soja en Brasil. Se proporcionan caracterizaciones morfológicas y moleculares, además de la verificación de la presencia de E. inaron en áreas infestadas por mosca blanca, lo que indica que la avispa puede potencialmente ser utilizada en programas de manejo integrado. Además de la verificación morfológica, el análisis molecular permitió identificar algún grado de variabilidad genética en el genoma del parasitoide.