Resumo Altas doses de antibióticos utilizados em hospitais podem afetar a composição microbiana dos esgotos, selecionando bactérias resistentes. Nesse sentido, avaliamos o perfil de resistência a antibióticos e o fenótipo multirresistente de bactérias isoladas em esgoto de um hospital terciário no interior do estado de São Paulo, Brasil. Para o isolamento de bactérias, foram semeados 10 µL das amostras de esgoto em meios de cultura seletivos e os isolados foram identificados usando o sistema automatizado VITEK-2. O teste de sensibilidade aos antibióticos foi realizado por disco-difusão em ágar. Elevadas porcentagens de resistência foram encontradas para amoxicilina, ampicilina, ceftazidima, clindamicina, vancomicina e o fenótipo multirresistente (MDR) foi atribuído a 60,7% dos isolados. Nossos resultados mostram bactérias classificadas como prioridade crítica/alta pela Lista de Patógenos Prioritários da OMS (Enterococcus e Staphylococcus aureus resistentes à vancomicina e Enterobacteriaceae resistentes aos carbapenêmicos) no esgoto hospitalar. Sendo assim, implementação de tecnologias de desinfecção do esgoto hospitalar reduziriam a carga bacteriana no esgoto que chegará às estações de tratamento de esgoto urbanas, minimizando a contaminação dos ecossistemas hídricos receptores e a disseminação da resistência bacteriana no ambiente.

Abstract High doses of antibiotics used in hospitals can affect the microbial composition of sewers, selecting resistant bacteria. In this sense, we evaluated the antibiotic resistance profile and the multiresistant phenotype of bacteria isolated in sewage from a tertiary hospital in the interior São Paulo state, Brazil. For bacteria isolation, 10 µL of sewage samples were sown in selective culture media and the isolates were identified using VITEK-2 automatized system. The antibiotic sensitivity test was performed by disk diffusion. High percentages of resistance were found for amoxicillin, ampicillin, ceftazidime, clindamycin, vancomycin and the multidrug-resistant phenotype (MDR) was attributed to 60.7% of the isolates. Our results show bacteria classified as critical/high priority by WHO List of Priority Pathogens (Enterococcus and Staphylococcus aureus resistant to vancomycin and Enterobacteriaceae resistant to carbapenems) in hospital sewage. Therefore, the implementation of disinfection technologies for hospital sewage would reduce the bacterial load in the sewage that will reach urban wastewater treatment plants, minimizing superficial water contamination and bacterial resistance spread in the environment.

Abstract Wilson’s Plover, Charadrius wilsonia, is widely distributed in coastal areas of the Americas. This report presents the first record of breeding in this species on Coroa do Avião Island, on the coast of Pernambuco, and in the estuary of the Cardoso and Camurupim rivers, on the coast of Piauí, in northeastern Brazil, extending the known area of reproduction of this species in this region. One breeding pair was observed on October 29th 2014 on Coroa do Avião Island, and a second pair was recorded on April 6th 2016 in the Cardoso/Camurupim estuary on the Piauí coast. Both the male and the female contributed to the incubation of the eggs. The nest on Coroa do Avião island was camouflaged by the local vegetation, but despite this, the eggs were attacked by a predator. Possible predators observed on the island included Caracara plancus and domestic cats and dogs. Wilsons Wilson s Plover wilsonia Americas Island Pernambuco rivers Brazil region th 201 CardosoCamurupim vegetation predator dogs 20 2

Resumo Charadrius wilsonia (Wilson’s Plover) está amplamente distribuído pela costa das Américas. Este é o primeiro registro de reprodução no litoral de Pernambuco, Coroa do Avião, e no estuário dos rios Cardoso e Camurupim, litoral do Piauí, Brasil, ampliando a área de reprodução no Nordeste do Brasil. Foi encontrado um casal em período reprodutivo em 23 de outubro de 2014 na ilha Coroa do Avião, litoral de Pernambuco e outro registro em 06 de abril de 2016 nos estuários Cardoso e Camurupim, litoral do Piauí. Foi observado que o macho e a fêmea contribuem na incubação dos ovos. A vegetação na ilha contribui para a camuflagem do ninho, bem como na proteção dos ovos pelos predadores. Apesar da proteção ocorreu a predação do ninho na ilha Coroa do Avião. Alguns possíveis predadores foram registrados na ilha, como Caracara plancus que diariamente frequentam a área e animais domésticos como cães e gatos. Wilsons Wilson s Plover Américas Avião Camurupim Piauí Brasil 2 201 0 gatos 20

We discuss the construction of Maxwellian electrodynamics in 2+1 dimensions and some of its applications. Special emphasis is given to the problem of the retarded potentials and radiation, where substantial differences with respect to the usual three-dimensional case arise. These stem from the general form of the solutions of the wave equation in two dimensions, which we discuss using the Green’s function method. We believe the topics presented here could be stimulating additions to an advanced electrodynamics course at the undergraduate level.

Background The international recommendations of antiretroviral treatment include resistance tests to guide the treatment regimen in each patient, which is not available on a regular basis in Ecuador. Aim To describe mutations that confer resistance to antiretrovirals in a population of Ecuadorian patients. Methods Plasma samples from 101 HIV-1 patients with failure to antiretroviral therapy, divided into 15 children and 86 adults, were studied with the GS Junior (Roche) and the sequences were analyzed with the DeepChek program. Results The most frequent mutations were M184V/I, K101E/P/H, K103N/S, D30N, M46L/I, I54L/M, V82T/F/A/S/L and L90M in adults and F77L, K103N/S, M46L/I, V82T/F/A/S/L and L90M in children. High resistance to non-nucleoside reverse transcriptase (RT) inhibitors in minority viral populations of adults and children (34.9% and 70%) was detected; in children both viral populations (majority and minority viral populations) (> 45%) were protease inhibitor resistant. Patients who had a greater number of therapeutic regimens had higher levels of resistance to antiretrovirals. Most of the samples were subtype B in the TR and protease region, and CRF25_cpx in integrase. Conclusions Mutations and resistance to antiretrovirals are shown in a population of Ecuadorian patients with HIV-1. These results will make it possible to issue a warning to health authorities about the need for resistance studies.

Resumen Introducción Las recomendaciones internacionales de tratamiento anti-retroviral incluyen pruebas de resistencia para orientar el régimen de tratamiento en cada paciente, lo que no está disponible de forma estable en Ecuador. Objetivo Describir las mutaciones que confieren resistencia a anti-retrovirales en una población de pacientes ecuatorianos. Metodología A partir de muestras de plasma de 101 pacientes con VIH-1 con fallo a la terapia anti-retroviral, 15 niños y 86 adultos, se realizó pirosecuenciación con el GS Junior (Roche) y se analizaron las secuencias con el programa DeepChek. Resultados Las mutaciones más frecuentes fueron M184V/I, K101E/P/H, K103N/S, D30N, M46L/I, I54L/M, V82T/F/A/S/L y L90M en adultos, y F77L, K103N/S, M46L/I, V82T/F/A/S/L y L90M en niños. Se encontró una elevada resistencia a los inhibidores de la transcriptasa reversa (TR) no análogos de nucleósidos en poblaciones minoritarias virales de adultos y niños (34,9 y 70%, respectivamente), en los niños, tanto las poblaciones virales mayoritarias como minoritarias, fueron resistente a inhibidores de proteasa (> 45%). Los pacientes que tuvieron un mayor número de esquemas terapéuticos presentaron mayores niveles de resistencia a los anti-retrovirales. La mayoría de las muestras fueron del subtipo B en la región de la TR y proteasa, y CRF25_cpx en integrasa. Conclusiones Se muestran las mutaciones y la resistencia a antiretrovirales en una población de pacientes ecuatorianos con infección por VIH-1, que permitirán realizar un llamado de alerta a las autoridades de salud sobre la necesidad de realizar estudios de resistencia.

Abstract Gold mining and ore processing are activities of great economic importance. However, they are related to generation of extremely polluted effluents containing high concentrations of heavy metals and low pH. This study aims to evaluate the optimal conditions for gold mining effluent treatment by crossflow membrane filtration regarding the following variables: nanofiltration (NF) and reverse osmosis (RO) membrane types, feed pH and permeate recovery rate. It was observed that retention efficiencies of NF90 were similar to those of RO membranes though permeate fluxes obtained were 7-fold higher. The optimum pH value was found to be 5.0, which resulted in higher permeate flux and lower fouling formation. At a recovery rate above 40% there was a significant decrease in permeate quality, so this was chosen as the maximum recovery rate for the proposed system. We conclude that NF is a suitable treatment for gold mining effluent at an estimated cost of US$ 0.83/m3.

Resumo O uso de graus-dia – utilizado em espécies cultivadas para predizer eventos e planejar ações de manejo – é reportado em poucos trabalhos para descrever a germinação de árvores tropicais. As temperaturas cardeais (base, ótima e teto) para germinação de uma espécie pode variar de acordo com a procedência das sementes. Peltophorum dubium (Spreng.) Taub. é uma leguminosa arbórea de sucessão inicial amplamente distribuída na América do Sul, podendo ocorrer como espécie cultivada ou naturalizada, sendo considerada um bom exemplo para testar modelos de graus-dia em espécie arbórea. O principal objetivo deste estudo foi descrever a resposta da germinação das sementes de diferentes populações de P. dubium como função do acúmulo de graus-dia durante ensaios em condições semi-controladas (flutuação térmica). Testes de germinação com sementes escarificadas manualmente e semeadas em bandejas de alumínio contendo substrato foram realizados sob condições de casa de vegetação em diferentes épocas do ano. Três métodos foram utilizados para descrever o acúmulo de tempo térmico nos ensaios e, considerando o lote das sementes e a época da semeadura, o método da área do trapézio foi relativamente mais eficaz em descrever a germinação. As curvas de germinação de sementes de P. dubium provenientes de diferentes populações, expressas em graus-dia estimados diretamente por meio do registro programado das temperaturas, tendem a ser mais agrupadas sugerindo pouca variação no requerimento de tempo térmico entre as diferentes procedências das sementes. Por outro lado, o requisito de tempo térmico pode variar de acordo com a época de semeadura e um incremento nos graus-dia exigidos quando os ensaios foram realizados sob temperaturas médias mais elevadas pode estar relacionado e um efeito térmico na germinação de sementes escarificadas.

Abstract Few works report the use of degree-days (DD) – used in crops to predict events and schedule management activities – to describe the germination of tropical trees. The cardinal temperatures (base, optimum and ceiling temperature) for germination of the species may vary depending on the seed provenance. Peltophorum dubium (Spreng.) Taub. is an early successional leguminous tree widely distributed in South America, often occurring as cultivated or naturalized trees, thus considered to be a good example for testing DD model in tree species. The main objective of this study was to describe the seed germination response of different populations of P. dubium as function of DD accumulation during germination assays in semi-controlled (fluctuating temperatures) conditions. Germination assays with manually scarified seeds sown in aluminum sheet trays filled with a composed substrate were performed under greenhouse conditions at different times. Three methods were employed in order to describe the accumulation of thermal time throughout the assays and, considering the seed lot and sowing time, a trapezoid area method was relatively more effective in describing the germination. The germination curves of P. dubium seeds from different populations, expressed in degree-days estimated directly from temperature records schedules, tend to be more clustered suggesting little variation among thermal time requirements in different seed provenances. Otherwise, the thermal time requirement can vary depending on the time of sowing, and any increase in DD requirement when the assays were performed under higher mean temperatures can be related to a thermal effect on the germination of scarified seeds.

Abstract This study evaluates aerobic and anaerobic biodegradability and toxicity of a real pharmaceutical wastewater, which focuses on antibiotics production. Zahn-Wellens and Organization for Economic Cooperation and Development (OECD) methodologies were applied in order to verify the wastewater's biodegradability and Microtox® analysis was performed for toxicity tests. Tests achieved more than 89% and 63% of Total Organic Carbon reduction, showing 80% and 50% of antibiotic removal, for aerobic and anaerobic processes, respectively. Moreover, acute ecotoxicological tests revealed that both techniques decreased the toxic character of real pharmaceutical wastewater. Desorption tests showed that the antibiotic was not degraded, but, in fact, adsorbed onto the sludge. Since biological treatment is the most widely used method for industrial wastewater treatment, this study indicates that this kind of treatment is probably unable to mineralize antibiotics present in pharmaceutical wastewaters, which may induce the development of resistant pathogens. Therefore, efforts must be taken to elucidate the main mechanisms of biological antibiotic removal from wastewaters since the presence of antibiotics in the environment is considered to be an emerging environmental issue.

Resumo O padrão de ocupação de conchas pelo ermitão D. insignis (Saussure, 1858) na região subtropical da costa sudeste do Brasil, foi investigada no presente estudo. Foram analisadas a percentual de tipos de conchas que foram ocupados e as relações morfométricas entre os ermitões e conchas ocupadas, a partir de coletas mensais realizadas durante dois anos (de janeiro de 1998 a dezembro de 1999). Os indivíduos foram classificados de acordo com o sexo e maturação, pesados e medidos em relação ao comprimento escudo cefalotoracico (CEC) e peso úmido (CPU). As conchas foram medidas em relação à sua largura de abertura (LAC), peso seco (PSC) e volume interno (VIC). Um total de 1.086 ermitões foram coletados, ocupando conchas de 11 espécies de gastrópodos. Olivancillaria urceus (Roding, 1798) foi a mais utilizada pelo ermitão D. insignis, seguido por Buccinanops cochlidium (Dillwyn, 1817), e Stramonita haemastoma (Linnaeus, 1767). Os maiores coeficientes de determinação (r2 > 0,50, p < 0,01) foram registrados principalmente nas relações morfométricas entre CEC e CPU contra LAC e VIC, que é uma importante indicação de que nesta população de D. insignis a grande maioria dos animais ocupavam conchas adequadas durante os dois anos analisados. O elevado número de espécies de conchas utilizadas e a relativa plasticidade no padrão de ocupação de conchas pelos menores indivíduos de D. insignis indicaram que a ocupação é influenciada pela disponibilidade de conchas, enquanto os indivíduos maiores demonstraram uma ocupação mais especializadas na concha de Tonna galea (Linnaeus, 1758).

Abstract The pattern of shell occupation by the hermit crab Dardanus insignis (Saussure, 1858) from the subtropical region of southeastern coast of Brazil was investigated in the present study. The percentage of shell types that were occupied and the morphometric relationships between hermit crabs and occupied shells were analyzed from monthly collections conducted during two years (from January 1998 to December 1999). Individuals were categorized according to sex and gonadal maturation, weighed and measured with respect to their cephalothoracic shield length (CSL) and wet weight (CWW). Shells were measured regarding their aperture width (SAW), dry weight (SDW) and internal volume (SIV). A total of 1086 hermit crabs was collected, occupying shells of 11 gastropod species. Olivancillaria urceus (Roding, 1798) was most commonly used by the hermit crab D. insignis, followed by Buccinanops cochlidium (Dillwyn, 1817), and Stramonita haemastoma (Linnaeus, 1767). The highest determination coefficients (r2 > 0.50, p < 0.01) were recorded particularly in the morphometric relationships between CSL vs. CWW and SAW vs. SIV, which are important indication that in this D. insignis population the great majority the animals occupied adequate shells during the two years analysed. The high number of used shell species and relative plasticity in pattern of shell utilization by smaller individuals of D. insignis indicated that occupation is influenced by the shell availability, while larger individuals demonstrated more specialized occupation in Tonna galea (Linnaeus, 1758) shell.

Abstract This study evaluated the technical and economic feasibility of membrane bioreactors (MBR) followed by nanofiltration (NF) for dairy wastewater treatment in order to reuse the treated effluent. It was observed that the MBR efficiently removed the organic matter and color of the feed effluent; however, due to the high concentration of dissolved solids in the permeate, it was necessary to use nanofiltration as a polishing step. The final treated effluent could be reused in the industry for cooling, steam generation and cleaning of external areas. A preliminary economic analysis showed the feasibility of the proposed system. The internal rate of return was greater than or equal to 32% when membrane lifespan was at least 2 years and the depreciation time was 15 years. The total cost of the proposed treatment system ranged from R$ 9.99/m3 to R$ 6.82/m3, depending on membrane lifespan.

Introduction: The solid-pseudopapillary tumor of pancreas (STP) is a low-grade malignant neoplasm. In Chile, 21 cases have been reported since 2008, most of them treated in Santiago. The present series contributes to the national case-load and has the purpose to describe the experience with this uncommon neoplasm in the IV Region. Patients and Methods: From January 2004 to March 2014, a total of 38 benign and malignant pancreatic neoplasms have been informed in the data-base of our Regional Pathology Service. We selected the biopsies informed as STP and 5 cases (13%) were found. The clinical records of these patients were retrospectively reviewed. The results were reported using descriptive statistics with central tendency measures and dispersion. Results: Most patients were women with a mean age of 44.8 years. All patients were studied with either abdominal ultrasound; computed tomography or magnetic resonance. In 2 patients the STP was located in the pancreatic tail, and they were submitted to distal pancreatectomy. The other 3 patients had the STP in the pancreatic head and were resolved by pancreatoduodenectomy. All tumors were confirmed by immunohistochemistry. Late follow-up showed malignant behavior in 1 patient while the other 4 patients are currently free of disease. Conclusions: The general characteristics of STP in the IV Region are similar to what is currently known. They present a high incidence compared with the habitually published incidence.

Introducción: El tumor sólido pseudopapilar del páncreas (TSP) es una neoplasia con bajo potencial maligno. En Chile se han reportado 21 casos desde el año 2008, 18 de ellos tratados en instituciones de Santiago. La presente serie además de contribuir a la casuística nacional, tiene como objetivo la descripción de la experiencia de la IV Región con el manejo de estos tumores. Pacientes y Métodos: Entre enero de 2004 y marzo de 2014 se informaron 38 biopsias de tumores pancreáticos benignos y malignos en la base de datos del Servicio de Patología Regional. Se seleccionaron las biopsias informadas como TSP, las que constituyen 5 casos (13%) y se revisaron las fichas clínicas en forma retrospectiva. Para el informe de los resultados se utilizó estadística descriptiva con medidas de tendencia central y dispersión. Resultados: El promedio de edad fue 44,8 años, siendo la mayoría mujeres. Los pacientes fueron estudiados con ecografía, tomografía computarizada y resonancia magnética. En 2 pacientes el TSP se localizaba en la cola del páncreas, estos pacientes fueron sometidos a pancreatectomía distal. En los otros 3 casos el TSP se encontraba en la cabeza del páncreas y fueron resueltos mediante pancreatoduodenectomía. Los TSP fueron confirmados por inmunohistoquímica. El comportamiento posterior fue benigno en 4 casos y maligno en 1 caso. Conclusiones: Las características de los TSP en la IV Región son similares a las conocidas y presentan una alta incidencia comparada con la habitualmente reportada.

Among the food industries, the dairy industry is considered to be the most polluting one because of the large volume of wastewater generated and its high organic load. In this study, an aerobic membrane bioreactor (MBR) was used for the treatment of wastewater from a large dairy industry and two hydraulic retention times (HRT), 6 and 8 hours, were evaluated. For both HRTs removal efficiencies of organic matter of 99% were obtained. Despite high permeate flux (27.5 L/h.m²), the system operated fairly stablely. The molecular weight distribution of feed, permeate and mixed liquor showed that only the low molecular weight fraction is efficiently degraded by biomass and that the membrane has an essential role in producing a permeate of excellent quality.

"Mastitis" refers to inflammation of the mammary gland. Despite the economic losses that it causes in dairy goats, there is scarce information about the epidemiological status of the disease in Mexico. The aim of this study was to develop a multiplex polymerase chain reaction (PCR) test to diagnose Staphylococcus agents involved in caprine mastitis. Milk samples from animals in intensive and semi-intensive production systems were taken from two farms located at Tequisquiapan, Queretaro, Mexico. The samples were obtained from healthy goats. The multiplex PCR was developed to identify coagulase-negative Staphylococcus and S. aureus. To achieve the identification of Staphylococcus spp, primers for the 16s rRNA region were used. Another two pair of primers were used for S. aureus, the first pair corresponded to the clfA gene and the second pair corresponded to the S. aureus coa gene. Multiplex PCR was performed in 30 milk samples and it was possible to detect S. aureus and coagulase-negative Staphylococcus. Further studies are needed to evaluate the sensitivity and specificity of this technique.

La mastitis se refiere a la inflamación de la glándula mamaria, a pesar de las pérdidas económicas que produce en cabras lecheras, existe poca información acerca de la situación epidemiológica de la enfermedad en México. El objetivo de este estudio fue desarrollar una reacción en cadena de la polimerasa (PCR) múltiple para el diagnóstico de Staphylococcus spp como agente involucrado en mastitis caprina. Se colectaron muestras de leche de cabras clínicamente sanas en sistemas de producción intensivo y semi-intensivo de dos granjas ubicadas en Tequisquiapan, Querétaro, México. La PCR múltiple se desarrolló para identificar a los Staphylococcus coagulasa-negativos (SCN) y a Staphylococcus aureus. Para lograr la identificación del género Staphylococcus spp se utilizó el par de iniciadores correspondientes a la región 16s RNAr, para el caso de S. aureus se utilizaron 2 pares de iniciadores, el primer par de iniciadores corresponden al gen clfA y el segundo par corresponden al gen coa de S. aureus. Se realizó la PCR múltiple a 30 muestras de leche de cabra en donde se pudo detectar a S. aureus y a SCN, sin embargo, estos estudios son preliminares por lo que se requiere de estudios posteriores para evaluar la sensibilidad y especificidad de esta técnica.

The objective of the present study was to determine the prevalence of electrolyte disturbances in AIDS patients developing acute kidney injury in the hospital setting, as well as to determine whether such disturbances constitute a risk factor for nephrotoxic and ischemic injury. A prospective, observational cohort study was carried out. Hospitalized AIDS patients were evaluated for age; gender; coinfection with hepatitis; diabetes mellitus; hypertension; time since HIV seroconversion; CD4 count; HIV viral load; proteinuria; serum levels of creatinine, urea, sodium, potassium and magnesium; antiretroviral use; nephrotoxic drug use; sepsis; intensive care unit (ICU) admission, and the need for dialysis. Each of these characteristics was correlated with the development of acute kidney injury, with recovery of renal function and with survival. Fifty-four patients developed acute kidney injury: 72% were males, 59% had been HIV-infected for >5 years, 72% had CD4 counts <200 cells/mm³, 87% developed electrolyte disturbances, 33% recovered renal function, and 56% survived. ICU admission, dialysis, sepsis and hypomagnesemia were all significantly associated with nonrecovery of renal function and with mortality. Nonrecovery of renal function was significantly associated with hypomagnesemia, as was mortality in the multivariate analysis. The risks for nonrecovery of renal function and for death were 6.94 and 6.92 times greater, respectively, for patients with hypomagnesemia. In hospitalized AIDS patients, hypomagnesemia is a risk factor for nonrecovery of renal function and for in-hospital mortality. To determine whether hypomagnesemia is a determinant or simply a marker of critical illness, further studies involving magnesium supplementation in AIDS patients are warranted.

O proteassomo é uma protease intracelular multicatalítica envolvida na regulação do ciclo e sinalização celular, apresentação antigênica e apoptose. Uma vez que inibidores do proteassomo promovem morte celular por apoptose, esses têm sido propostos como novas drogas anti-tumorais. A terreína, um metabólito secundário secretado pelo fungo Aspergillus terreus, foi primeiramente descrita em 1935. Neste trabalho demonstramos que a terreína, isolada através da bioprospecção de inibidores do proteassomo, mostrou efeito inibitório das atividades do tipo quimiotripsina e tripsina da unidade catalítica do proteassomo 20S. Apesar da alta concentração inibitória determinada in vitro, aquela verificada após incubação de células em cultura na presença de terreína (fibroblasto e tumor pulmonar humano) foi 4 vezes menor, o que sugere que o proteassomo seja um alvo intracelular específico. A terreína promoveu morte celular por apoptose nas duas linhagens ensaiadas. Embora as concentrações de terreína necessárias para desencadear apoptose nos modelos celulares aqui testados tenham sido altas (ordem de mM) quando comparadas com doses utilizadas de outros inibidores descritos recentemente (ordem de μM e nM), sua estrutura química não está relacionada a nenhum outro inibidor conhecido até o momento. Concluímos que estes resultados apontam para a possibilidade de explorar a terreína como um novo fragmento molecular para o desenvolvimento de inibidores sintéticos do proteassomo.

The proteasome is an intracellular multicatalytic protease involved in the cell cycle regulation, signaling response, antigen presentation and apoptosis. Since proteasome inhibitors promote cell death by apoptosis, they have been proposed as new anti-tumoral drugs. Terrein, a secondary metabolite secreted by the fungus Aspergillus terreus, was firstly described in 1935. In the present work we report that terrein isolated through the screening for inhibitors of the 20S proteasome showed inhibitory effect upon both chymotrypsin- and trypsin-like activities of the multicatalytic core particle, the 20S proteasome. Despite of the high inhibitory concentration determined in vitro, that verified by incubating cells (fibroblasts and a pulmonary tumor cell line) in the presence of terrein was 4-fold lower indicating the proteasome as a selective intracellular target. Moreover, terrein promoted apoptotic cell death on both fibroblasts and pulmonary tumor cell line tested. Although terrein concentrations (mM range) necessary to elicit apoptosis in the cellular models herein tried were high when compared to those (μM and nM range) of other inhibitors recently described, its chemical structure is not correlated to any other inhibitor reported thus far. Therefore, the present results point out for the possibility of exploring terrein as a new molecular fragment for the development of synthetic proteasome inhibitors.

Mechanical ventilation has been associated with organ failure in patients with acute respiratory distress syndrome. The present study examines the effects of tidal volume (V T) on renal function using two V T values (8 and 27 mL/kg) in anesthetized, paralyzed and mechanically ventilated male Wistar rats. Animals were randomized into two groups of 6 rats each: V T8 (V T, 8 mL/kg; 61.50 ± 0.92 breaths/min; positive end-expiratory pressure, 3.0 cmH2O; peak airway pressure (PAW), 11.8 ± 2.0 cmH2O), and V T27 (V T, 27 mL/kg; 33.60 ± 1.56 breaths/min; positive end-expiratory pressure, none, and PAW, 22.7 ± 4.0 cmH2O). Throughout the experiment, mean PAW remained comparable between the two groups (6.33 ± 0.21 vs 6.50 ± 0.22 cmH2O). For rats in the V T27 group, inulin clearance (mL·min-1·body weight-1) decreased acutely after 60 min of mechanical ventilation and even more significantly after 90 min, compared with baseline values (0.60 ± 0.05 and 0.45 ± 0.05 vs 0.95 ± 0.07; P < 0.001), although there were no differences between groups in mean arterial pressure or gasometric variables. In the V T8 group, inulin clearance at 120 min of mechanical ventilation remained unchanged in relation to baseline values (0.72 ± 0.03 vs 0.80 ± 0.05). The V T8 and V T27 groups did not differ in terms of serum thiobarbituric acid reactive substances (3.97 ± 0.27 vs 4.02 ± 0.45 nmol/mL) or endothelial nitric oxide synthase expression (94.25 ± 2.75 vs 96.25 ± 2.39%). Our results show that glomerular filtration is acutely affected by high tidal volume ventilation but do not provide information about the mechanism.