The selective separation of precious metals from complex aqueous solutions is important in protecting the environment, aquatic ecosystems, water resources and recycling resources. In this study, cysteine-containing polymeric nanoparticles were synthesized using the molecular imprinting (MIP) technique with the mini-emulsion polymerization method. The basis of the polymer design is inspired by affinity of silver to cysteine in biological systems. The synthesized silver ion imprinted (Ag+-IIP) poly(hydroxyethylmethacrylate-N -methacryloyl-L-cysteine) poly(HEMA-MAC) nanoparticles were characterized by elemental analysis, zeta sizer, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). The effects of pH, ionic strength and interaction time on adsorption of Ag+ ions were investigated. In experimental studies, the highest adsorption amount (196.9 mg g1 nanoparticle) was reached in a short period of 40 min at pH 5.0 and 150 mg L-1 concentration. Selectivity studies of synthesized Ag+-IIP nanoparticles against silver (Ag+) ions were carried out in the presence of lithium (Li+), barium (Ba2+), mercury (Hg2+) and cadmium (Cd2+) ions. Experimental results indicate that nanoparticles adsorb Ag+ ions with high selectivity. Ag+-IIP nanoparticles have 3.7, 3.1, 2.4, and 2.6 times more selective or higher affinity for Ag+ ions compared to Li+, Ba2+, Hg2+ and Cd2+ ions, respectively, compared to Ag+-non-imprinted (NIP) nanoparticles. environment ecosystems study cysteinecontaining containing MIP (MIP miniemulsion mini emulsion method systems Ag+IIP AgIIP IIP Ag (Ag+-IIP polyhydroxyethylmethacrylateN poly hydroxyethylmethacrylate N methacryloylLcysteine methacryloyl L -methacryloyl-L-cysteine polyHEMAMAC HEMA MAC poly(HEMA-MAC analysis sizer FTIR (FTIR spectroscopy AFM, AFM , (AFM) SEM. SEM . (SEM) investigated 196.9 1969 196 9 (196. g nanoparticle 4 50 5 0 5. 15 L1 1 L- concentration (Ag+ Li Li+ (Li+) Ba2 Ba Ba2+ (Ba2+) Hg2 Hg (Hg2+ Cd2 Cd (Cd2+ selectivity 37 3 7 3.7 31 3.1 24 2 2.4 26 6 2. respectively Ag+nonimprinted Agnonimprinted non NIP (NIP (AFM (SEM 196. 19 (196 (Ag (Li+ (Ba2+ (Hg2 (Cd2 3. nonimprinted (19 (Li (Ba2 (Hg (Cd (1 (Ba (

ABSTRACT: Anticarsia gemmatalis (Lepidoptera: Erebidae) is distributed in tropical and subtropical regions of America, and is an important pest of Fabaceae, such as: soybean, peanut, common bean, cowpea, pea, chickpea and kudzu. In soybean, the velvet bean caterpillar is important due to the plant defoliation in their larval stage. This study evaluated soybean cultivars as source of resistance to A. gemmatalis by antixenosis (attractiveness and non-preference for feeding) and antibiosis (biological parameters of the insect). The parameters evaluated were: antixenosis: attractiveness and non-preference, dry mass consumed and attractiveness index and antibiosis: duration of larval and pupal stages, adult longevity, total cycle, larval and pupal weights and larval, pupal and total viability. Considering antixenosis and antibiosis the least suitable cultivars for A. gemmatalis were found to be BRS 8383 IPRO, BRS 1074 IPRO, BRS 1061 IPRO, BRS 7180 IPRO, BRS 9383 IPRO, BRS 8980 IPRO and BRS 1003 IPRO due to high mortality in the larval phase. The cultivars BRS 523 and BRS 543 RR “block technology” suggest displays antixenosis and or antibiosis to A. gemmatalis. These cultivars can be used by soybean producers in combination with other control tactics in soybean IPM. ABSTRACT Lepidoptera (Lepidoptera Erebidae America Fabaceae peanut cowpea pea kudzu stage A nonpreference non preference feeding biological insect. insect . insect) nonpreference, preference, stages longevity cycle viability 838 107 106 718 938 898 100 phase 52 54 block technology IPM 83 10 71 93 89 5 8 1 7 9

RESUMO: Anticarsia gemmatalis (Lepidoptera: Erebidae) está distribuída nas regiões tropicais e subtropicais da América, trata-se de importante praga de Fabaceae, como: soja, amendoim, feijão, feijão-caupi, ervilha, grão de bico e kudzu. Na soja, a lagarta-da-soja é importante devido à desfolha da planta em sua fase larval. O objetivo deste trabalho foi avaliar cultivares de soja como fonte de resistência a A. gemmatalis por antixenose (atratividade e não-preferência para alimentação) e antibiose (parâmetros biológicos do inseto). Os parâmetros avaliados foram: antixenose: atratividade e não-preferência, massa seca consumida e índice de atratividade e antibiose: duração dos estágios larval e pupal, longevidade de adultos, ciclo total, peso larval e pupal e viabilidade larval, pupal e total. Na antixenose e antibiose os cultivares menos favoráveis a A. gemmatalis foram: BRS 8383 IPRO, BRS 1074 IPRO, BRS 1061 IPRO, BRS 7180 IPRO, BRS 9383 IPRO, BRS 8980 IPRO e BRS 1003 IPRO devido à alta mortalidade larval. Os cultivares BRS 523 e BRS 543 RR “Tecnologia block” apresentaram antixenose e/ou antibiose a A. gemmatalis. Essas cultivares podem serem utilizadas por produtores de soja em combinação com outras táticas de controle no MIP. RESUMO Lepidoptera (Lepidoptera Erebidae América tratase trata se Fabaceae amendoim feijão feijãocaupi, feijãocaupi caupi, caupi feijão-caupi ervilha kudzu lagartadasoja lagarta A nãopreferência não preferência alimentação inseto. inseto . inseto) foram nãopreferência, preferência, adultos total 838 107 106 718 938 898 100 52 54 Tecnologia block eou ou MIP 83 10 71 93 89 5 8 1 7 9

The total concentrations and the bioaccessible fractions of Al, B, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, V and Zn were evaluated in 10 samples of pseudocereals, being 3 of amaranth, 3 of quinoa and 4 of chia. The samples were decomposed in a digester block with a reflux system under conditions optimized and the analyses were performed by microwave-induced plasma optical emission spectrometry. In all grains investigated, the highest total concentrations were found for the macroelements K, Mg and Ca, as well as the microelements Fe, Mn, and Zn. When the bioaccessible fraction was evaluated, negative correlations were observed between the content of total phenolic compounds and the bioaccessibility for most elements, in all samples. Among the elements considered essential in our diet, K (99%) and Cu (59%) presented the highest percentages of bioaccessible fraction. Among those elements considered potentially toxic, Ba did not exceed 15% bioaccessible fraction and Al presented concentrations lower than the limit of detection in all samples. B Ca Fe Mn Na Ni 1 pseudocereals amaranth chia microwaveinduced microwave induced spectrometry investigated diet 99% 99 (99% 59% 59 (59% toxic 15 9 (99 5 (59 (9 (5 (

ABSTRACT Introduction: The analysis of patients submitted to heart surgery at three assessment times has been insufficiently described in the literature. Objective: To analyze chest expansion, maximum inspiratory pressure (MIP), maximum expiratory pressure (MEP), distance traveled on the six-minute walk test (6MWT), and quality of life in the preoperative period, fourth postoperative day (4th PO), and 30th day after hospital discharge (30th-day HD) in individuals submitted to elective heart surgery. Methods: A descriptive, analytical, cross-sectional study was conducted with 15 individuals submitted to elective heart surgery between 2016 and 2020 who did not undergo any type of physiotherapeutic intervention in Phase II of cardiac rehabilitation. The outcome variables were difference in chest expansion (axillary, nipple, and xiphoid), MIP, MEP, distance on 6MWT, and quality of life. The assessment times were preoperative period, 4th PO, and 30th-day HD. Results: Chest expansion diminished between the preoperative period and 4th PO, followed by an increase at 30th-day HD. MIP, MEP, and distance traveled on the 6MWT diminished between the preoperative period and 4th PO, with a return to preoperative values at 30th-day HD. General quality of life improved between the preoperative period and 4th PO and 30th-day HD. An improvement was found in the social domain between the preoperative period and the 30th-day HD. Conclusion: Heart surgery causes immediate physical deficit, but physical functioning can be recovered 30 days after hospital discharge, resulting in an improvement in quality of life one month after surgery. Introduction literature Objective MIP , (MIP) MEP (MEP) sixminute six minute MWT (6MWT) th PO) 30thday thday HD Methods descriptive analytical crosssectional cross sectional 1 201 202 rehabilitation axillary, axillary (axillary nipple xiphoid, xiphoid xiphoid) Results Conclusion deficit 3 (MIP (MEP (6MWT 20 2

More attention has been paid to immunotherapy for ovarian cancer and the development of tumor vaccines. We developed a trichostatin A (TSA)-modified tumor vaccine with potent immunomodulating activities that can inhibit the growth of ovarian cancer in rats and stimulate immune cell response in vivo. TSA-treated Nutu-19 cells inactivated by X-ray radiation were used as a tumor vaccine in rat ovarian cancer models. Prophylactic and therapeutic experiments were performed with TSA-modified tumor vaccine in rats. Flow cytometry and ELISpot assays were conducted to assess immune response. Immune cell expression in the spleen and thymus were detected by immunohistochemical staining. GM-CSF, IL-7, IL-17, LIF, LIX, KC, MCP-1, MIP-2, M-CSF, IP-10/CXCL10, MIG/CXCL9, RANTES, IL-4, IFN-γ, and VEGF expressions were detected with Milliplex Map Magnetic Bead Panel immunoassay. TSA vaccination in therapeutic and prophylactic models could effectively stimulate innate immunity and boost the adaptive humoral and cell-mediated immune responses to inhibit the growth and tumorigenesis of ovarian cancer. This vaccine stimulated the thymus into reactivating status and enhanced infiltrating lymphocytes in tumor-bearing rats. The expression of key immunoregulatory factors were upregulated in the vaccine group. The intensities of infiltrating CD4+ and CD8+ T cells and NK cells were significantly increased in the vaccine group compared to the control group (P<0.05). This protection was mainly dependent on the IFN-γ pathway and, to a much lesser extent, by the IL-4 pathway. The tumor cells only irradiated by X-ray as the control group still showed a slight immune effect, indicating that irradiated cells may also cause certain immune antigen exposure, but the efficacy was not as significant as that of the TSA-modified tumor vaccine. Our study revealed the potential application of the TSA-modified tumor vaccine as a novel tumor vaccine against tumor refractoriness and growth. These findings offer a better understanding of the immunomodulatory effects of the vaccine against latent tumorigenesis and progression. This tumor vaccine therapy may increase antigen exposure, synergistically activate the immune system, and ultimately improve remission rates. A vaccine strategy designed to induce effective tumor immune response is being considered for cancer immunotherapy. vaccines TSAmodified modified vivo TSAtreated treated Nutu19 Nutu 19 Nutu-1 Xray X ray staining GMCSF, GMCSF GM CSF, CSF GM-CSF IL7, IL7 IL 7, 7 IL-7 IL17, IL17 17, 17 IL-17 LIF LIX KC MCP1, MCP1 MCP 1, 1 MCP-1 MIP2, MIP2 MIP 2, 2 MIP-2 MCSF, MCSF M M-CSF IP10/CXCL10, IP10CXCL10 IPCXCL IP 10/CXCL10, 10 CXCL10 CXCL IP-10/CXCL10 MIGCXCL9 MIGCXCL MIG CXCL9 MIG/CXCL9 RANTES IL4, IL4 4, 4 IFNγ, IFNγ IFN γ, γ immunoassay cellmediated mediated tumorbearing bearing CD4 CD CD8 P<0.05. P005 P P<0.05 . 0 05 (P<0.05) extent IL- effect exposure progression system rates Nutu1 Nutu- IL1 IL-1 MCP- MIP- IP10 IP10/CXCL10 IP10CXCL1 10CXCL10 10/CXCL10 CXCL1 IP-10/CXCL1 MIG/CXCL P00 P<0.0 (P<0.05 IP1 IP10/CXCL1 IP10CXCL 10CXCL1 10/CXCL1 IP-10/CXCL P0 P<0. (P<0.0 IP10/CXCL 10CXCL 10/CXCL P<0 (P<0. P< (P<0 (P< (P

Modelos dinâmicos de equilíbrio geral computável (EGC) são uma ferramenta de simulação e análise dos potenciais efeitos da implementação de políticas sobre o comportamento dos agentes, o fluxo de renda e o bem-estar das famílias. Esses modelos são alimentados pelos dados disponíveis em uma Matriz de Contabilidade Social (MCS), a qual traz muita informação da Matriz Insumo Produto (MIP). Este trabalho busca validar um modelo EGC - construído nos moldes de Hosoe et al. (2016) - que utiliza como fonte de dados duas MIPs: a do NEREUS e a do IBGE. A intenção é responder duas perguntas: i) quão adequada é a estrutura do modelo para representar a economia brasileira? e, ii) qual fonte de dados deve alimentar o modelo para que ele se torne mais acurado? Essa validação do modelo foi avaliada com base nas MCSs projetadas 5 anos para frente quando comparadas aos dados do IBGE para tal ponto no tempo. Os resultados sugerem que a prescrição do modelo tem bastante aderência aos dados observados e que a MIP do IBGE seria mais adequada para ser utilizada como fonte de dados. Classificação JEL. E270, O210. (EGC agentes bemestar bem estar famílias MCS, MCS , (MCS) MIP. . (MIP) al 2016 (2016 MIPs perguntas i brasileira ii acurado tempo JEL E270 E O210 O (MCS (MIP 201 (201 E27 O21 20 (20 E2 O2 2 (2 (

ABSTRACT Currently, agricultural cropping systems have adopted integrated pest management (IPM) as a successful model for pest control. The use of entomopathogenic fungi in IPM has increased because of their great potential for reducing arthropod pest populations without causing harm to human health and ecosystems. Beauveria bassiana and Metarhizium anisopliae stand out among the most used fungi in biological control, with many isolates commercialized worldwide. Helicoverpa armigera is an agriculturally important pest in Brazil and worldwide, causing damage to several crops. In this context, the objective of this study was to evaluate the biological control potential of Beauveria bassiana, Metarhizium anisopliae, and M. rileyi on H. armigera. The results indicated that the entomopathogenic fungi B. bassiana (strain ESALQ PL63), M. anisopliae (strain ESALQ E9), and M. rileyi (strain UFMS 03), applied using different methods (direct application and dry film) and concentrations (1 × 107, 1 × 108, and 1 × 109 conidia mL-1), resulted in low mortality and no virulence in first-, third-, and fifth-instar larvae of H. armigera. The death of all individuals subjected to treatment with B. bassiana (strain ESALQ PL63) was confirmed by conidiogenesis. Considering the importance of H. armigera as an agricultural pest and the biodiversity of entomopathogenic fungi in Brazil, further investigations on the virulence of fungal strains are necessary to improve the integrated management of lepidopteran pests through microbial control, explore the potential of new strains, and understand the relationships between microorganisms and host defense mechanisms. Currently (IPM ecosystems worldwide crops context M H B strain PL63, PL63 PL , E9, E9 E E9) 03, 03 03) direct film ( 107 108 10 mL1, mL1 mL mL-1) first, first first- third, third third- fifthinstar fifth instar conidiogenesis mechanisms PL6 0 mL-1 mL-

RESUMO Atualmente, os sistemas de cultivo agrícola adotam o manejo integrado de pragas (MIP) como modelo de sucesso para o controle de pragas. O uso de fungos entomopatogênicos tem aumentado no manejo integrado devido ao seu grande potencial em reduzir as populações de pragas agrícolas sem causar danos à saúde humana e aos ecossistemas. Beauveria bassiana e Metarhizium anisopliae são as espécies de fungos mais utilizadas no controle biológico, contando com muitos isolados comercializados no mundo. Helicoverpa armigera é uma praga de importância agrícola, causadora de prejuízos associados a diversas culturas. Assim, esse estudo teve como objetivo avaliar o potencial virulento de Beauveria bassiana, Metarhizium anisopliae e Metarhizium rileyi no controle biológico dos estágios larvais de H. armigera. Os resultados indicaram que em diferentes concentrações (1 × 107, 1 × 108 e 1 × 109 con.mL-1) e métodos de aplicação (DA ou DF) B. bassiana (cepa ESALQ PL63), M. anisopliae (cepa ESALQ E9) e M. rileyi (cepa UFMS 03) causaram baixa mortalidade e não foram virulentos ao primeiro, terceiro e quinto ínstares de H. armigera. A mortalidade pelo tratamento com B. bassiana (cepa ESALQ PL63) foi confirmada por conidiogênese em todos os cadáveres. Dada a importância de H. armigera como praga agrícola e a biodiversidade de fungos entomopatogênicos no Brasil, novas investigações sobre a virulência de cepas fúngicas são necessárias para aprimorar o manejo integrado de pragas através do controle microbiano, o potencial para novas cepas e o entendimento das relações entre microrganismos e os mecanismos de defesa dos hospedeiros. Atualmente MIP (MIP ecossistemas mundo culturas Assim H ( 107 10 con.mL1 conmL1 conmL con.mL con mL con.mL-1 DA DF B cepa PL63, PL63 PL , M E9 E 03 primeiro cadáveres Brasil microbiano hospedeiros mL1 con.mL- PL6 0

Resumo Carrapatos são artrópodes parasitos que causam perdas econômicas significativas na produção de bovinos em todo o mundo. Embora o carrapato bovino - Rhipicephalus (Boophilus) microplus - esteja distribuído em todo o território brasileiro, não há um programa oficial de controle do parasito. Nesta revisão, foi abordada a situação da resistência de R. (B.) microplus aos carrapaticidas sintéticos no Brasil, incluindo: controle do carrapato; estado da resistência por estado; histórico da ocorrência de resistência a diferentes carrapaticidas; resistência múltipla; e as principais estratégias para o manejo integrado de carrapatos. No Brasil, laboratórios locais empregam empregam, inicialmente, o teste de imersão de adultos como método diagnóstico de resistência aos carrapaticidas. Apenas três estados (Acre, Amapá e Amazonas) não têm relatos de populações resistentes. O controle de carrapatos, no Brasil, é caracterizado por erros de manejo, há desinformação sobre estratégias de controle de carrapatos, uso inadequado dos produtos disponíveis, falta de adoção de práticas de manejo integrado de parasitos (MIP), baixo suporte técnico aos produtores e a rápida emergência de populações de carrapatos multirresistentes a carrapaticidas. Ao final, propõe-se uma lista de necessidades e prioridades para o controle de carrapatos em bovinos com relação à comunicação, pesquisa e políticas públicas. mundo Boophilus (Boophilus brasileiro parasito revisão R B. B (B. Brasil incluindo múltipla inicialmente Acre, Acre (Acre Amazonas resistentes disponíveis MIP, MIP , (MIP) final propõese propõe se comunicação públicas (B (MIP

Abstract Ticks are parasitic arthropods that cause significant economic losses to livestock production worldwide. Although Rhipicephalus (Boophilus) microplus, the cattle tick, occurs throughout the Brazilian territory, there is no official program to control this tick, which is the vector of tick fever pathogens. We address the situation of R. (B.) microplus resistance to synthetic acaricides in Brazil, including cattle tick management; the status of tick resistance per Brazilian state; the history of resistance occurrence of different acaricides; multiple resistance occurrence; and the main strategies for integrated tick management. Tick control in Brazil is characterized by management errors. Local laboratories affiliated with federal and state research institutions and universities employ the Adult Immersion Test as a primary diagnostic method to assess acaricide resistance to topically applied drugs. Only three states (Acre, Amapá, and Amazonas) have no reports on resistant populations. Misinformation on tick control strategies, misuse of available products for tick control, no adoption of Integrated Parasite Management (IPM) practices, low technical support to producers, and the high-speed emergence of acaricide-resistant tick populations are the main problems. We also propose a list of needs and priorities for cattle tick control regarding communication, research, and policies. worldwide Boophilus (Boophilus territory pathogens R B. B (B. errors drugs Acre, Acre (Acre Amapá Amazonas IPM (IPM practices producers highspeed high speed acaricideresistant problems communication policies (B

Abstract Introduction Chronic obstructive pulmonary disease (COPD) not only restricts airflow but also induces sys-temic manifestations in individuals with the disease. Objective To evaluate the effect of a water-based aero-bic exercise program on respiratory muscle strength, thoracic mobility, dyspnea, and functional capacity in patients with COPD. Methods We conducted a blind randomized controlled trial with 22 patients with COPD, dividing them into a control group (CG) and a training group (TG). The TG participated in 24 sessions of a water aerobic exercise program, while the CG only partici-pated in the evaluations. Maximal respiratory pressure (MRP), dyspnea, and functional capacity were measured. Results When comparing the MRP values (cmH2O) in the pre- and post-training conditions, the results revealed a significant improvement in the TG [maximal inspiratory pressure (MIP): 74.8 ± 15.3 vs. 83.9 ± 17.2; maximal expi-ratory pressure (MEP): 141.5 ± 30.7 vs. 157.6 ± 32.9], whereas no difference was observed for the CG (MIP: 55.5 ± 21.8 vs. 54.4 ± 18.4; MEP: 116.2 ± 40.3 vs. 109.3 ± 38.9). Regarding thoracic mobility in the pre- and post-training conditions, no significant difference was found for the CG, whilst for the TG there was a significant in-crease at the axillary level (cm) (5.9 ± 1.8 vs. 7.7 ± 1.1). With respect to functional capacity, there was a signifi-cant increase in walking distance during the six-minute walking test only in the TG when comparing pre- and post-training conditions (462.1 ± 62.9 vs. 538.5 ± 63.7). Lastly, the dyspnea results demonstrated that after the training period there was a major reduction in the scores of Medical Research Council (3.1 ± 0.8 vs. 1.9 ± 0.7) and Borg CR-10 scales (5.2 ± 0.8 vs. 3.7 ± 0.3) only for the TG. Conclusion The water aerobic exercise training promoted beneficial changes in respiratory mus-cle strength, thoracic mobility, functional capacity and dyspnea among patients with COPD. COPD (COPD systemic sys temic waterbased based aero bic strength 2 (CG . (TG) partici pated evaluations MRP, , (MRP) measured cmH2O cmHO cmH O (cmH2O pre posttraining post MIP (MIP) 748 74 8 74. 153 15 3 15. vs 839 83 9 83. 17.2 172 17 expiratory expi ratory MEP (MEP) 1415 141 5 141. 307 30 7 30. 1576 157 6 157. 32.9, 329 32.9 32 32.9] (MIP 555 55 55. 218 21 21. 544 54 4 54. 18.4 184 18 1162 116 116. 403 40 40. 1093 109 109. 38.9. 389 38.9 38 38.9) crease cm (cm 5.9 59 (5. 1 1. 77 7. 1.1. 11 1.1 1.1) signifi cant sixminute six minute 462.1 4621 462 (462. 629 62 62. 5385 538 538. 63.7. 637 63.7 63 63.7) Lastly 3.1 31 (3. 08 0 0. 19 0.7 07 CR10 CR 10 CR-1 5.2 52 37 3. 0.3 03 mus cle (TG (MRP 17. (MEP 14 32. 18. 38. 5. (5 462. 46 (462 53 63. (3 CR1 CR- ( (46 (4

Resumo Introdução A doença pulmonar obstrutiva crônica (DPOC) não apenas restringe o fluxo aéreo, mas também induz mani-festações sistêmicas em indivíduos com a doença. Objetivo Avaliar o efeito de um programa de exercícios aeróbicos aquáticos na força muscular respiratória (FMR), mobilidade torácica, dispneia e capacidade funcional em pacientes com DPOC. Métodos Realizou-se um ensaio clínico randomizado cego com 22 pacientes com DPOC, divi-dindo-os em grupo controle (GC) e grupo treinamento (GT). O GT participou de 24 sessões de um programa de exercícios aeróbicos aquáticos, enquanto o GC participou somente das avaliações. Foram me-didas a pressão respiratória máxima, (PRM) dispneia e capa-cidade funcional. Resultados Ao comparar os valores da PRM (cmH2O) nas condições pré e pós-treinamento, os resultados revelaram melhora significativa no GT [pressão inspiratória má-xima (PImáx): 74,8 ± 15,3 vs. 83,9 ± 17,2; pressão expiratória máxima (PEmáx): 141,5 ± 30,7 vs. 157,6 ± 32,9], enquanto não observou-se diferença para o GC (PImáx: 55,5 ± 21,8; vs. 54,4 ± 18,4; PEmáx: 116,2 ± 40,3 vs. 109,3 ± 38,9). Em relação à mobilidade torácica nas condições pré e pós-treinamento, não foi encontrada diferença significativa para o GC, enquanto para o GT houve um aumento significante no nível axilar (cm) (5,9 ± 1,8 vs. 7,7 ± 1,1). Com relação à capacidade funcional, houve aumento significativo da distância percorrida durante o teste de caminhada de 6 minutos apenas no GT quando comparadas as condições pré e pós-treinamento (462,1 ± 62,9 vs. 538,5 ± 63,7). Por fim, os resultados da dispneia demonstraram que após o período de treinamento houve uma redução importante nas pontuações do Medical Research Council (3,1 ± 0,8 vs. 1,9 ± 0,7) e nas escalas Borg CR-10 (5,2 ± 0,8 vs. 3,7 ± 0,3) apenas para o GT. Conclusão O trei-namento físico aquático promoveu alterações benéficas na força muscular respiratória, mobilidade torácica, capacidade funcional e dispneia em pacientes com DPOC. DPOC (DPOC aéreo manifestações mani festações FMR, FMR , (FMR) Realizouse Realizou se 2 dividindoos divi dindo (GC . (GT) avaliações medidas me didas (PRM capa cidade cmH2O cmHO cmH (cmH2O póstreinamento, póstreinamento pós treinamento, má xima PImáx (PImáx) 748 74 8 74, 153 15 3 15, vs 839 83 9 83, 17,2 172 17 PEmáx (PEmáx) 1415 141 5 141, 307 30 7 30, 1576 157 157, 32,9, 329 32,9 32 32,9] observouse observou (PImáx 555 55 55, 21,8 218 21 544 54 4 54, 18,4 184 18 1162 116 116, 403 40 40, 1093 109 109, 38,9. 389 38,9 38 38,9) cm (cm 5,9 59 (5, 1 1, 77 7, 1,1. 11 1,1 1,1) 462,1 4621 462 (462, 629 62 62, 5385 538 538, 63,7. 637 63,7 63 63,7) fim 3,1 31 (3, 08 0 0, 19 0,7 07 CR10 CR 10 CR-1 5,2 52 37 3, 0,3 03 trei namento (FMR (GT 17, (PEmáx 14 32, 21, 18, 38, 5, (5 462, 46 (462 53 63, (3 CR1 CR- ( (46 (4

In the present study, a novel magnetic dispersive solid-phase extraction method using molecularly imprinted polymer synthesized through semi-covalent imprinting approach was developed for cholesterol (CHO) determination in milk samples by high-performance liquid chromatography with diode array detector (HPLC-DAD). The adsorbent named Fe3O4/SiO2/MIP (MIP: molecularly imprinted polymers) was prepared through sol-gel polymerization of 3-(triethoxysilyl)propyl isocyanate (ICPTES) covalently bonded to CHO with tetraethoxysilane (TEOS), in the presence of Fe3O4/SiO2 particles. The adsorbent was characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS) and scanning electron microscopy (SEM). The proposed molecularly imprinted polymer-based magnetic dispersive solid-phase extraction method (MIP-MDSPE) was based on vortex-assisted preconcentration of 10.0 mL of CHO solution in chloroform as donor phase using 10.0 mg of Fe3O4/SiO2/MIP during 60 s. After this step, the magnetic particles were collected using a permanent magnet and the vortex-assisted elution was carried with 500 µL of ethanol during 180 s. A wide analytical curve ranging from 38.5 to 25000 µg L-1 (determination coefficient (R2) = 0.998) and low limits of detection (LOD) and quantification (LOQ) 11.5 and 38.5 µg L 1, respectively, were obtained. Milk samples (whole and reduced-fat milk) were subjected to MIP-MDSPE method after saponification reaction followed by CHO extraction with chloroform. Using external calibration, the CHO concentration ranged from 99.2 (reduced-fat milk) to 397.6 mg kg 1 (whole milk) and the accuracy was attested by addition and recovery tests (92 to 97%). study solidphase solid semicovalent semi covalent (CHO highperformance high performance HPLCDAD. HPLCDAD HPLC DAD . (HPLC-DAD) Fe3O4SiO2MIP FeOSiOMIP Fe3O4 SiO2 MIP Fe O SiO (MIP polymers solgel sol gel 3triethoxysilylpropyl triethoxysilylpropyl 3 triethoxysilyl propyl ICPTES (ICPTES TEOS, TEOS , (TEOS) Fe3O4SiO2 FeOSiO Fe3O4/SiO ATRFTIR, ATRFTIR ATR FTIR (ATR-FTIR) Xray X ray XRD, XRD (XRD) DLS (DLS SEM. SEM (SEM) polymerbased MIPMDSPE MDSPE (MIP-MDSPE vortexassisted vortex assisted 100 10 0 10. 6 s step 50 18 385 38 5 38. 2500 L1 L- R2 R (R2 0.998 0998 998 LOD (LOD LOQ (LOQ 115 11 11. respectively obtained whole reducedfat reduced fat calibration 992 99 2 99. 3976 397 397. 92 (9 97%. 97 97% 97%) (HPLC-DAD FeO Fe3O (TEOS Fe3O4SiO (ATR-FTIR (XRD (SEM 250 (R 0.99 099 9 39 ( 25 0.9 09 0.

ABSTRACT Background. The euphotic zone (EUZ) is the most superficial section of the water column where solar radiation is sufficient to produce photosynthesis. In situ depth is measured using light profiler probes but can also be monitored using remote sensing. Objective. To determine the temporal and spatial variability of Bahía de Banderas Euphotic Zone (EUZ) and its relationship with other variables that contribute to turbidity. Methods. Different data sources (OceanColor, Copernicus, NOAA, CONAGUA) were used to obtain time series (2003 to 2020) of particulate inorganic matter (PIM), Chlorophyll-a (Chl-a), diffuse attenuation coefficient at 490 nm (Kd490), depth of the Secchi disk (ZSD), ZEU, daily precipitation and water level of the Ameca and Pitillal rivers and a correlation analysis was made between these variables, and a cluster analysis to determine if there are spatial differences in the bay, as well as scatter plots to observe trends among environmental parameters. Two seasonal maps (rainy and dry season) were drawn based on the climatic medians of ZEU and the prevailing geostrophic currents. Results. The euphotic zone (EUZ) was deeper from June to August (48.54 ± 21.93 m) and shallower from February to April (22.95 ± 12.98 m); the cluster analysis determined that the bay is divided into two areas: the north, whose ZEU is shallower (higher correlation with MIP and Chl-a), and the south, where it is deeper (higher correlation with Chl-a). Conclusions. The parameter that has the greatest effect on the depth of the ZEU is the Chl-a, and only the area closest to the mouth of the Ameca and Pitillal rivers presents an inverse relationship between the ZEU with the MIP and the Chl-a.

RESUMEN Antecedentes. La zona eufótica (ZEU) es la sección más superficial de la columna de agua donde la radiación solar es suficiente para producir fotosíntesis. La profundidad in situ se mide usando sondas perfiladoras de la luz, pero también se puede monitorear utilizando percepción remota. Objetivo. Determinar la variabilidad temporal y espacial de la zona eufótica (ZEU) de Bahía de Banderas y su relación con otras variables que contribuyen a la turbidez. Métodos. Se utilizaron distintas fuentes de datos (OceanColor, Copernicus, NOAA, CONAGUA), para obtener series de tiempo (2003 al 2020) de materia inorgánica particulada (MIP), Clorofila-a (Chl-a), coeficiente de atenuación difusa a 490 nm (Kd490), profundidad del disco de Secchi (ZSD), ZEU, precipitación diaria y nivel hídrico de los ríos Ameca y Pitillal, se hizo un análisis de correlación entre estas variables. A partir de lo anterior, se realizó un análisis de conglomerados para determinar si existen diferencias espaciales en la bahía, así como diagramas de dispersión para observar las tendencias entre los parámetros ambientales. Se realizaron dos mapas estacionales (lluvias y secas) con base a las medianas climáticas de ZEU y a las corrientes geostróficas predominantes. Resultados. La profundidad de la zona eufótica (ZEU) fue mayor de junio a agosto (48.54 ± 21.93 m) y menor de febrero a abril (22.95 ± 12.98 m); el análisis de conglomerado determinó que la bahía se divide en dos áreas: la norte cuya ZEU es más somera (mayor correlación con la MIP y Chl-a), y la sur, donde es más profunda (mayor correlación con la Chl-a). Conclusiones. El parámetro que tiene mayor efecto sobre la profundidad de la ZEU es la Chl-a, y solamente el área más cercana a la desembocadura de los ríos Ameca y Pitillal presenta relación inversa entre la ZEU con la MIP y la Chl-a.

RESUMEN Introducción: Las enfermedades cardiovasculares son la principal causa mundial de muerte. Mediante la educación se pueden prevenir sus factores de riesgo. Objetivo: Evaluar el nivel de conocimiento sobre factores de riesgo cardiovascular antes y después de una intervención educativa en médicos internos de pregrado. Método: Estudio experimental realizado en octubre de 2021, en el cual participaron 45 médicos internos de pregrado (MIP). De forma aleatoria se formó un grupo de intervención y uno de control. La estrategia educativa fue un curso-taller sobre factores de riesgo cardiovascular. Se obtuvieron frecuencias simples, comparación de medias y análisis bivariante. Resultados: En más del 50% de los MIP se identificó sedentarismo, sobrepeso u obesidad, consumo de alcohol, antecedente familiar de diabetes mellitus y de enfermedad cardiovascular. Posterior a la intervención, la media de calificación del grupo de intervención fue 8.11 y del grupo control 4.92 (diferencia de medias: 3.1; t: 7.6; p = 0.00). Los alumnos incluidos en la estrategia educativa tuvieron mayor probabilidad de obtener un mejor nivel de conocimiento (OR: 19.6; IC95%: 3.5-110; c2 M-H: 12.3; p = 0.001). Conclusión: Realizar una intervención educativa sobre factores de riesgo cardiovascular en MIP mejora significativamente su nivel de conocimiento.

ABSTRACT Introduction: Cardiovascular diseases are the main cause of death worldwide. Through education their risk factors can be prevented. Objective: To evaluate the level of knowledge about cardiovascular risk factors before and after an educational intervention in undergraduate interns. Method: Experimental study carried out in October 2021, 45 undergraduate internal doctors participated; an intervention group and a control group were randomly formed. The educational strategy was a course-workshop on cardiovascular risk factors. Simple frequencies, comparison of means and bivariate analysis were obtained. Results: Sedentary lifestyle, overweight or obesity, alcohol consumption, family history of diabetes mellitus and cardiovascular disease were identified in more than 50% of the undergraduate internal doctors. After the intervention, the mean score for the intervention group was 8.11 and 4.92 for the control group (mean difference: 3.1; t: 7.6; p = 0.00). The students included in the educational strategy were more likely to obtain a better level of knowledge (OR: 19.6; 95%CI: 3.5-110; c2 M-H: 12.3; p = 0.001). Conclusion: Carrying out an educational intervention on cardiovascular risk factors in undergraduate internal doctors significantly improves their level of knowledge.

Abstract This case-control study evaluated the gene expression levels of interleukin (IL)-4, macrophage inflammatory protein type 1 alpha (MIP-1α), and metalloproteinase (MMP)-9, factors involved in the formation of giant cells in healthy peri-implant tissue and peri-implantitis. Thirty-five subjects (15 healthy and 20 with peri-implantitis), who met the inclusion and exclusion criteria, were included in this study. The peri-implant tissue biopsies were subjected to total RNA extraction, DNAse treatment, and cDNA synthesis. Subsequently, the reaction of real-time PCR was performed to evaluate the gene expression levels of IL-4, MIP-1α, and MMP-9 concerning the reference gene. IL-4 gene expression showed higher (18-fold) values in the Peri-Implantitis Group of Patients when compared with the Healthy (Control) Group (p<0.0001). Although MIP- 1α and MMP-9 gene expression levels were higher in diseased implants, they showed no significant differences (p=0.06 and p=0.2337), respectively. Within the limitations of this study, the results showed that in tissues affected by peri-implantitis, only levels of Il-4 were increased when compared with tissues in the control group. casecontrol case IL4, IL4 IL 4, 4 (IL)-4 MIP1α, MIP1α MIPα MIP , α (MIP-1α) MMP9, MMP9 MMP 9, 9 (MMP)-9 periimplant peri implant periimplantitis. periimplantitis implantitis. implantitis peri-implantitis Thirtyfive Thirty five 15 (1 2 periimplantitis, peri-implantitis) criteria extraction treatment synthesis Subsequently realtime real time 1α, MIP-1α MMP- IL- 18fold fold 18 (18-fold PeriImplantitis Peri Implantitis Control (Control p<0.0001. p00001 p p<0.0001 . 0 0001 (p<0.0001) implants p=0.06 p006 06 (p=0.0 p=0.2337, p02337 p=0.2337 2337 p=0.2337) respectively implantitis, Il4 Il Il- group (IL)- (MIP-1α (MMP)- ( p0000 p<0.000 000 (p<0.0001 p=0.0 p00 (p=0. p0233 p=0.233 233 (IL) (MMP) p000 p<0.00 00 (p<0.000 p=0. p0 (p=0 p023 p=0.23 23 (IL (MMP p<0.0 (p<0.00 p=0 (p= p02 p=0.2 p<0. (p<0.0 p= (p p<0 (p<0. p< (p<0 (p<

Resumo Este estudo caso-controle teve como objetivo avaliar a expressão gênica dos níveis de interleucina (IL)-4, proteína inflamatória de macrófagos tipo alfa 1 (MIP-1α) e metalopreoteinase (MMP)-9, todos fatores envolvidos na formação de células gigantes em tecidos peri-implantares saudáveis e com peri-implantite. Trinta e cinco indivíduos (15 saudáveis e 20 com peri-implantite) foram incluídos nesse estudo seguindo os critérios de inclusão e exclusão. Os tecidos peri-implantares foram submetidos a extração do RNA total, tratamento de DNAse e síntese de cDNA. Subsequentemente, a reação de PCR em tempo real foi realizada para avaliar os níveis da expressão de IL-4, MIP-1α, e MMP-9 em relação ao gene de referência. O nível de expressão de IL-4 foi estatisticamwente maior (18 vezes) nos tecidos de pacientes com peri-implantite quando comparados aos pacientes saudáveis (grupo controle) (p<0,0001). Embora os níveis de expressão de MIP- 1α e MMP-9 apresentassem maiores valores nos implantes doentes, esses níveis não foram estatisticamente significantes (p=0.06 and p=0.2337) respectivamente. Dentro das limitações desse estudo, os resultados mostraram que nos tecidos afetados pela peri-implantite, apenas os nívies de IL-4 estavam aumentados quando comparados ao grupo controle. casocontrole caso controle IL4, IL4 IL 4, 4 (IL)-4 MIP1α MIPα MIP α (MIP-1α MMP9, MMP9 MMP 9, 9 (MMP)-9 periimplantares peri implantares periimplantite. periimplantite implantite. implantite 15 (1 2 exclusão total cDNA Subsequentemente MIP1α, 1α, MIP-1α MMP- referência IL- 18 vezes p<0,0001. p00001 p p<0,0001 . 0 0001 (p<0,0001) doentes p=0.06 p006 06 (p=0.0 p=0.2337 p02337 2337 respectivamente periimplantite, implantite, (IL)- (MMP)- ( p0000 p<0,000 000 (p<0,0001 p=0.0 p00 (p=0. p=0.233 p0233 233 (IL) (MMP) p000 p<0,00 00 (p<0,000 p=0. p0 (p=0 p=0.23 p023 23 (IL (MMP p<0,0 (p<0,00 p=0 (p= p=0.2 p02 p<0, (p<0,0 p= (p p<0 (p<0, p< (p<0 (p<

Resumen Objetivo: Analizar los componentes subyacentes de las presiones inspiratorias (MIP) y espiratorias (MEP) es táticas máximas reducidas en sujetos con distrofia de Duchenne (DMD). Métodos: Se evaluaron 43 pacientes mediante pruebas de función pulmonar rutinarias. MIP y MEP fueron medidas a inspiración y espiración máximas. Los volúmenes pulmonares se midieron mediante dilución de helio. Resultados: Edad media 13 años (rango 7-20 años). La capacidad pulmonar total (TLC) y el volumen residual (RV) fueron 78.0% (49.0-94.0) y 27.0% (19.7- 30.1) de los valores predichos. El RV/TLC fue de 35.3% (28.1-47.7). Treinta y cinco sujetos tenían una TLC por debajo del límite inferior de normalidad, 31 tenían una RV/TLC por encima del límite superior de la normalidad. MIP y MEP fueron -53.0 (-65.5 a -41.8) y 58.0 (41.5-74.8) cmH2O, mientras que en % de los predichos (TLC y RV predichos) fueron 42.6 (33.3-50.8) y 33.7 (23.9-44.5). MIP en % del RV alcanzado (Grupo A 7-11 años) fue mayor (p 0.025), y MEP en % de la TLC alcanzada Grupo B (12-16 años) y C (17-20 años), también fue mayor (0.031). Conclusiones: En sujetos con DMD, debilidad intrínseca de los músculos respiratorios y desventaja mecánica conducen a generación de presión estática máxima inadecuada. Las mismas deben interpretarse con cautela, ya que sobrestiman la debilidad de los músculos respiratorios si se las compara con las tablas de valores predichos obtenidos a TLC y RV. Nuestros resultados proporcionan datos adicionales que respaldan la utilización de valores absolutos en lugar de los predichos.

Abstract Objective: To analyze the underlying components of reduced maximal static inspiratory (MIP) and expiratory (MEP) pressures in subjects with Duchenne muscular dystrophy. Methods: Forty-three subjects were assessed based on routine pulmonary function tests. MIP and MEP were measured the subjects performed maximal expirations and inspirations using a snorkel mouthpiece. Lung volumes were measured us ing the helium dilution technique. Results: The mean age was 13 years (range, 7-20 years). Median total lung capacity (TLC) and residual volume (RV) were 78.0 (49.0-94.0) and 27.0 (19.7-30.1) of the predicted values re spectively. The RV/TLC relationship was 35.3% (28.1-47.7). Thirty-five subjects had a TLC below the lower limit of normal, while 31 had an RV/TLC ratio above the upper limit of normal. The median (IQR) MIP and MEP values were -53.0 (-65.5 to -41.8) and 58.0 (41.5-74.8) cmH2O respectively. MIP and MEP in percent of the predicted values (predicted TLC and RV) were 42.6 (33.3-50.8) and 33.7 (23.9-44.5). MIP in percent of the RV reached for Group A (7-11 years old) was higher (p 0.025) while MEP in percent of the TLC reached for Group B (12-16 years) and C (17-20 years) were higher too (0.031). Conclusions: In subjects with Duchenne muscular dystrophy, the intrinsic weakness of respiratory muscles and mechanical disadvantage lead to inadequate maximal static pressure generation. Maximal static pressures should be interpreted cautiously as they overestimate respiratory muscle weakness when compared to predicted values obtained at TLC and RV. Our results provide additional data supporting absolute values use rather than predicted values.

Resumo: O artigo caracterizou a adoção do Manejo Integrado de Pragas (MIP) na produção paulista de laranja a partir do LUPA 2016/2017. Para tanto, buscou apoio nas construções teóricas e conceituais sobre mudança tecnológica e MIP, para conduzir análise pautada no cenário de produção, comercialização, organizacional e de P&D da cultura da laranja, assim como do tratamento e análise de informações censitárias, LUPA 2016/2017, no recorte do MIP e da produção de laranja. Os resultados apontam o forte posicionamento internacional do suco de laranja brasileiro e paulista, associado a uma ampla e consolidada estrutura de representação da agroindústria e da produção agrícola, bem como das atividades públicas e privadas de P&D. Esses resultados convivem com a limitada oferta de tecnologias para solução dos principais problemas fitossanitários da cultura. Nesse ambiente, o MIP toma espaço como alternativa viável e importante, estando presente em 40% da área e pés em produção, em propriedades em que a laranja é a principal fonte de renda familiar, com proprietários que possuem maior nível educacional e são cooperados ou associados. O MIP também foi relacionado a outras tecnologias, a exemplo do uso de mudas certificadas e do crédito e seguro rural.

Abstract: The article characterized the adoption of Integrated Pest Management (IPM) in orange production in São Paulo from LUPA 2016/2017. To this end, it sought support in theoretical and conceptual constructions on technological change and MIP, to conduct an analysis based on the production, commercialization, organizational and R&D scenario of the orange crop, as well as the treatment and analysis of census information, LUPA 2016/ 2017, in the cut of MIP and orange production. The results point to the strong international positioning of Brazilian and São Paulo orange juice, associated with a broad and consolidated structure of representation of agro-industry and agricultural production, as well as public and private R&D activities. These results coexist with the limited supply of technologies to solve the main phytosanitary problems of the crop. In this environment, MIP takes place as a viable and important alternative, being present in 40% of the area and plants in production, in properties where orange is the main source of family income, with owners who have a higher educational level and are cooperative or associated. . The MIP was also related to other technologies, such as the use of certified seedlings and rural credit and insurance.