Species from Pavonia genus (Malvaceae) are traditionally used as antioxidant, n anti inflammatory and antimicrobial remedies. Previous studies have revealed their diversity of specialized metabolites widely used in the development of new medicines. This is the first study on Pavonia glazioviana Gurke, a Brazilian species known as “Malva da Chapada”. The research led to identify fifteen compounds: 132 (S) hydroxy pheophytin a; and 132(S) hydroxy 173 ethoxyphaeophorbide, n decanol, cycloart 23Z ene 3β,25 diol, cycloart 24S 25 ene 3β,24 diol, sitosterol 3 O β D glucopyranoside, stigmasterol 3 O β D glucopyranoside, quercetin, acacetin, kaempferol, tiliroside, 5 hydroxy 3,7,8,4’ tetramethoxyflavone, 5,7 dihydroxy 3,8,4’ trimethoxyflavone, 5,7 dihydroxy 4’ methoxyflavone, 5,7,4’ trihydroxy 3,8 dimethoxyflavone and 5,7,4’ trihydroxy 3 methoxyflavone. The major flavonoids were tested for their antimicrobial activity, and the compound 5,7 dihydroxy 3,8,4’ trimethoxyflavone showed strong activity against Escherichia coli (MIC = 512 μg mL1), Pseudomonas aeruginosa (MIC = 512 μg mL1), Candida albicans (MIC = 512 μg mL1), Candida tropicalis (MIC = 512 μg mL1), Candida parapsilosis (MIC = 512 μg mL1), Aspergillus flavus (MIC = 512 μg mL1) and A. fumigantus (MIC = 512 μg mL1). The total phenolic content, flavonoid content, and radical scavenging potential were determined showing its great production of phenolics and flavonoids, along with interesting radical scavenging potential (DPPH EC50 = 6.36 ± 0.029 mg mL 1). Our findings contributed to characterize P. glazioviana as a source of methoxylated flavonoids with antimicrobial potential. Malvaceae (Malvaceae antioxidant remedies medicines Gurke Malva Chapada. Chapada . Chapada” compounds 13 S (S 132S 132(S 17 ethoxyphaeophorbide decanol Z 3β25 3β 3β,2 diol 2 3β24 24 glucopyranoside quercetin acacetin kaempferol tiliroside 3,7,8,4 3784 7 8 4 tetramethoxyflavone 57 5, 3,8,4 384 methoxyflavone 5,7,4 574 38 3, MIC 51 mL1, mL1 , A mL1. content DPPH EC EC5 636 6 36 6.3 0029 0 029 0.02 1. 1 1) P 3β2 3β, 3,7,8, 378 3,8, 5,7, 63 6. 002 02 0.0 3,7,8 37 00 0. 3,7, 3,7

Protium ovatum Eng. Burseraceae is an endemic species in Brazil used in traditional medicine. This study investigated the chemical variation and the in vitro antioxidant and antimicrobial properties of dichloromethane soluble fraction of hydrolate (DSFH) from leaves of P. ovatum collected from five different vegetation physiognomies within the Cerrado Goiano. The region was characterized based on the DSFH chemical composition by gas chromatography coupled to mass spectrometry (GC-MS) analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were performed to compare the different DSFHs from five Savannah regions in Brazil. The DSFHs from the Cerrado stricto sensu (CSS), Mata de Galeria (MG) and Cerradão (C) regions were grouped in the negative part of PC1, showing high similarity. Samples from the C and MG regions showed antioxidant activity of IC50 = 0.98 and 0.78 mg mL-1, respectively, and 0.250 mg mL-1 of butylated hydroxytoluene (positive control). DSFH from different regions exhibited antifungal activity against Candida albicans. The results show that the chemical composition and the biological activity of DSFHs can be affected according to the area of collection. Eng medicine (DSFH P Goiano GCMS GC MS (GC-MS PCA (PCA HCA (HCA CSS, CSS , (CSS) (MG (C PC1 PC similarity IC IC5 098 0 98 0.9 078 78 0.7 mL1, mL1 mL 1, 1 respectively 0250 250 0.25 mL- positive control. control . control) albicans collection (CSS 09 9 0. 07 7 025 25 0.2 02 2

Abstract Escherichia coli causes colibacillosis in farm animals that act as reservoirs for pathogenic strains. Antimicrobial resistance of E. coli producing Extended Spectrum Beta-lactamases [ESBL] is a serious public health problem that can be attributed to factors related to food consumption and contact with domestic animals. This study aimed to determine the presence and patterns of antimicrobial resistance of ESBL E. coli isolated from fecal samples from dairy cattle in the Pichincha province. A total of 182 bovine feces samples were analyzed, 112 samples from cattle slaughtered at the official slaughterhouse in Quito-Pichincha province, and 70 samples from the collection of the Foodborne Diseases and Antimicrobial Resistance Research Unit [UNIETAR]. The isolation of ESBL E. coli, biochemical identification, and resistance tests using the main antibiotics were carried out at UNIETAR. It was possible to identify 93 positive samples for ESBL E. coli (51%), phenotypic analysis revealed that antibiotics such as amoxicillin plus clavulanic acid, cefepime, ceftazidime, ciprofloxacin, amikacin, tetracycline, presented resistance higher than 80%. Furthermore, low resistance to nitrofurantoin, cefoxitin, and ertapenem was observed, while no isolate was resistant to tigecycline. One hundred percent of the isolates presented multi-resistance phenotypes, with the most frequent pattern being composed of 7 families of antibiotics. In conclusion, these results suggest that E. coli from dairy cattle origin could be an important reservoir of ESBL genes.

Resumen La bacteria Escherichia coli causa la colibacilosis en animales de granja que actúan como reservorios de cepas patógenas. La resistencia antimicrobiana de E. coli productor de betalactamasas de espectro extendido [BLEE] es un grave problema de salud pública y se puede atribuir a factores relacionados con el consumo de alimentos y el contacto con animales domésticos. El objetivo de este estudio fue determinar la presencia y patrones de resistencia antimicrobiana de E. coli BLEE aislado en muestras fecales provenientes de bovinos productores de leche de la provincia de Pichincha. Se analizaron un total 182 muestras de heces de bovinos: 112 muestras de bovinos faenados en el Camal Metropolitano de la provincia de Pichincha y 70 muestras de la colección de la Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencias a los Antimicrobianos [UNIETAR], se realizó el aislamiento de E. coli BLEE, la identificación bioquímica y pruebas de resistencia a los principales antibióticos utilizados. Se logró identificar 93 muestras positivas a E. coli BLEE (51 %), el análisis fenotípico reveló que los antibióticos amoxicilina más ácido clavulánico, cefepime, ceftazidima, ciprofloxacina, amikacina y tetraciclina presentaron porcentajes de resistencia mayores al 80 %. Además, se observó una baja resistencia a la nitrofurantoína, cefoxitin y ertapenem, mientras que ningún aislado fue resistente a la tigeciclina. El 100 % de los aislados presentaron fenotipos de multirresistencia y el patrón más frecuente estuvo compuesto por 7 familias de antibióticos. En conclusión, estos resultados sugieren que E. coli originaria de bovinos lecheros podría ser un reservorio de genes BLEE.

Abstract Objective: Signs and symptoms of osteomyelitis or septic arthritis in neonates and infants are often nonspecific and early-stage bone infections in infants may often go unnoticed. The objective of this study was to analyze the clinical characteristics of newborns and infants with osteomyelitis and septic arthritis to improve understanding of the disorder and to assist clinicians with diagnosis. Methods: A retrospective multicenter study was conducted on neonates (0-28 days old, n = 94) and infants (1–12 months old, n = 415) with osteoarticular infections. Data consisting of clinical characteristics, complications, laboratory outcomes, and the pathogenic microorganisms causing osteomyelitis were tabulated. The statistics were further broken down into two regions and the significant differences between neonates and infants were evaluated and compared to the literature. Results: Compared to infants, neonates had significantly lower incidences of fever (p < 0.0001), higher incidences of localized swelling (p = 0.0021), higher rate of infection at the humerus (p = 0.0016), higher percentage of Escherichia coli (p < 0.0001) and Klebsiella pneumoniae (p = 0.0039) infections, lower percentage of Staphylococcus aureus infections (p < 0.0001) and were more likely to develop septic arthritis (p < 0.0001). Conclusion: Distinct differences were found between neonatal and infants with osteoarticular infections. Future studies should focus on improving diagnosis and subsequent treatment regimens for younger age groups. Objective earlystage early stage unnoticed Methods 028 0 28 (0-2 old 94 1–12 112 1 12 (1–1 415 complications outcomes tabulated literature Results p 0.0001, 00001 0.0001 , 0001 0.0021, 00021 0.0021 0021 0.0021) 0.0016, 00016 0.0016 0016 0.0016) 0.0039 00039 0039 0.0001. . Conclusion groups 02 2 (0- 9 1–1 11 (1– 41 0000 0.000 000 0002 0.002 002 0.001 001 0.003 0003 003 (0 1– (1 4 0.00 00 ( 0.0 0.

ABSTRACT: The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichiacoli colonizing the nasal and rectal cavities of Amazonian manatees kept in captivity at the National Institute for Amazonian Research (INPA) in the state of Amazonas, Brazil. Rectal and nasal swabs from 44 manatees of different ages were used in this study. The genus Staphylococcus was isolated from the nasal swabs of 32 (72.7%) animals, with two individuals harboring more than one species of Staphylococcus. S. sciuri was the most commonly isolated species. Resistance to penicillin was observed in 13 (40.6%) isolates, more frequent than the other antimicrobials tested (P = 0.01). E. coli was isolated from the rectal swabs of all animals, with phylogroup B1 being the most frequent among the strains obtained (P = 0.0008). Four isolates (6.8%) were positive for virulence factors, three of which were classified as enterotoxigenicE. coli (ETEC) and one as enteropathogenicE. coli (EPEC). To our knowledge, this is the first study to evaluate Staphylococcus spp. and E. coli in Amazonian manatee samples. This study revealed nasal colonization by Staphylococcus spp., mainly S. sciuri, and diarrheagenicE. coli isolates, including antimicrobial-resistant strains. ABSTRACT Trichechusinunguis (Trichechusinunguis extinction However health frequency distribution spp INPA (INPA Amazonas Brazil 4 3 72.7% 727 72 7 (72.7% animals S 1 40.6% 406 40 6 (40.6% P 0.01. 001 0.01 . 0 01 0.01) E B 0.0008. 00008 0.0008 0008 0.0008) 6.8% 68 8 (6.8% factors enterotoxigenicE ETEC (ETEC enteropathogenicE EPEC. EPEC (EPEC) knowledge samples diarrheagenicE antimicrobialresistant resistant 72.7 (72.7 40.6 (40.6 00 0.0 0000 0.000 000 6.8 (6.8 (EPEC 72. (72. 40. (40. 0. 0.00 6. (6. (72 (40 (6 (7 (4 (

RESUMO: O peixe-boi amazônico (Trichechus inunguis) é um mamífero aquático ameaçado de extinção. Apesar disso, poucos estudos investigaram patógenos nessa espécie que podem impactar a saúde animal e humana. O objetivo deste estudo foi avaliar a frequência, distribuição e padrões de suscetibilidade antimicrobiana de Staphylococcus sp. e Escherichia coli pertencentes à microbiota nasal e retal de peixes-bois da Amazônia mantidos em cativeiro no Instituto Nacional de Pesquisas da Amazônia (INPA) (Amazonas, Brasil). Foram utilizados suabes retais e nasais de 44 peixes-boi de diferentes idades. O gênero Staphylococcus foi isolado do swab nasal de 32 (72,7%) animais, sendo que dois indivíduos apresentaram mais de uma espécie de Staphylococcus. S. sciuri foi a espécie mais comumente isolada. A resistência à penicilina foi observada em 13 (40,6%) isolados, sendo mais frequente do que os outros antimicrobianos testados (P = 0,01). Escherichia coli foi isolada dos suabes retais de todos os animais, sendo o filogrupo B1 o mais frequente entre as cepas obtidas (P = 0,0008). Quatro isolados (6,8%) foram positivos para fatores de virulência, três dos quais foram classificados como E. coli enterotoxigênica (ETEC) e um isolado como E. coli enteropatogênica (EPEC). Este é o primeiro estudo a avaliar Staphylococcus spp. e E. coli de amostras de peixe-boi da Amazônia. O estudo revelou colonização nasal por Staphylococcus sp., principalmente S. sciuri, e por isolados diarreiogênicos de E. coli, incluindo cepas resistentes a antimicrobianos. RESUMO peixeboi peixe boi Trichechus inunguis extinção disso humana frequência sp peixesbois peixes bois INPA (INPA Amazonas, Amazonas (Amazonas Brasil. Brasil . Brasil) 4 peixesboi idades 3 72,7% 727 72 7 (72,7% animais S 1 40,6% 406 40 6 (40,6% P 0,01. 001 0,01 0 01 0,01) B 0,0008. 00008 0,0008 0008 0,0008) 6,8% 68 8 (6,8% virulência E ETEC (ETEC EPEC. EPEC (EPEC) spp 72,7 (72,7 40,6 (40,6 00 0,0 0000 0,000 000 6,8 (6,8 (EPEC 72, (72, 40, (40, 0, 0,00 6, (6, (72 (40 (6 (7 (4 (

ABSTRACT: The aim of this study is the determination some quality parameters of grape and mulberry pestils (leathers) sold in Elazig. For this purpose, a total of 45 fruit pestil samples, of which 23 were grape pestils and 22 were mulberry pestils, were analyzed for their physical properties, chemical properties, and microbiological characteristics. In grape and mulberry pestils, the average L* (lightness/darkness) values were 42.72 and 44.81, a* (redness) values were 10.29 and 9.07, and b* (yellow) values were 20.90 and 20.90, respectively. In grape and mulberry fruit pestil samples, the thickness values were 1.62 and 1.34, pH values were 3.82 and 4.75, acidity values (as citric acid) were 0.63 and 0.22%, aw values were 0.602 and 0.627, dry matter values were 68.78% and 72.99 %, ash values were 2.25% and 0.64%, insoluble ash values in 10% HCl were 0.20 and 0.29, and Brix values were 18.83 and 19.36, respectively. TMAB, coliform, Staphylococcus-Micrococcus, and yeast-mold counts were found to be 4.72 and 4.48, 1.30 and 1.89, 2.11 and 2.12, and 2.04 and 2.11 log10 cfu/g, respectively, in all samples examined. In 14 of the samples (31.11%), the total number of mesophilic aerobic microorganisms was found to be >5 log10 cfu/g. Escherichia coli and Salmonella spp. were not found in any of the samples. However, the samples were found to contain significant levels of yeast-mold. There was no difference between the groups in microbiological terms in statistical analysis (P > 0.05). According to the current study, there was no standard production and not enough hygiene rules were observed in the pestil production process in family businesses in Elazig. On the other hand, essential information was obtained on some physical, chemical and microbiological quality parameters of grape and blackberry pulps sold in markets and stores in Elazig. ABSTRACT leathers (leathers Elazig purpose 4 2 properties characteristics L lightness/darkness lightnessdarkness lightness darkness (lightness/darkness 4272 42 72 42.7 4481 44 81 44.81 redness (redness 1029 10 29 10.2 907 9 07 9.07 b yellow (yellow 2090 20 90 20.9 respectively 162 1 62 1.6 134 34 1.34 382 3 82 3.8 475 75 4.75 as acid 063 0 63 0.6 022 0.22% 0602 602 0.60 0627 627 0.627 6878 68 78 68.78 7299 99 72.9 % 225 25 2.25 064 64 0.64% 020 0.2 029 0.29 1883 18 83 18.8 1936 19 36 19.36 TMAB coliform StaphylococcusMicrococcus, StaphylococcusMicrococcus Staphylococcus Micrococcus, Micrococcus Staphylococcus-Micrococcus yeastmold yeast mold 472 4.7 448 48 4.48 130 30 1.3 189 89 1.89 211 11 2.1 212 12 2.12 204 04 2.0 log log1 cfug cfu g cfu/g examined 31.11%, 3111 31.11% , 31 (31.11%) 5 spp However yeastmold. mold. P 0.05. 005 0.05 . 05 0.05) hand 427 7 42. 8 44.8 102 10. 9.0 209 20. 16 6 1. 13 38 3. 47 06 0. 02 0.22 060 60 062 0.62 687 68.7 729 72. 2.2 0.64 188 18. 193 19.3 4. 4.4 1.8 21 2. 311 31.11 (31.11% 00 0.0 44. 9. 68. 19. 31.1 (31.11 31. (31.1 (31. (31 (3 (

RESUMO: O objetivo deste estudo é a determinação de alguns parâmetros de qualidade de pestilos de uva e amoreira (couros) vendidos em Elazig. Para tanto, um total de 45 amostras de pestil de frutas, sendo 23 de uva e 22 de amoreira, foram analisadas quanto às suas propriedades físicas, químicas e microbiológicas. Em pestils de uva e amoreira, os valores médios de L* (claridade/escuridão) foram 42.72 e 44.81, os valores de a* (vermelhidão) foram 10.29 e 9.07, e os valores de b* (amarelo) foram 20.90 e 20.90, respectivamente. Nas amostras de pestil de uva e amoreira, os valores de espessura foram 1.62 e 1.34, os valores de pH foram 3.82 e 4.75, os valores de acidez (como ácido cítrico) foram 0.63 e 0.22%, os valores de aw foram 0,602 e 0.627, os valores de matéria seca foram 68.78% e 72.99%, os valores de cinzas foram 2.25% e 0.64%, os valores de cinzas insolúveis em HCl a 10% foram 0.20 e 0.29, e os valores Brix foram 18.83 e 19.36, respectivamente. As contagens de TMAB, coliformes, Staphylococcus-Micrococcus e fungos de levedura foram 4.72 e 4.48, 1.30 e 1.89, 2.11 e 2.12 e 2.04 e 2.11 log10 ufc/g, respectivamente, em todas as amostras examinadas. Em 14 das amostras (31.11%), o número total de microrganismos aeróbios mesófilos foi >5 log10 ufc/g. Escherichia coli e Salmonella spp. não foram encontrados em nenhuma das amostras. No entanto, verificou-se que as amostras continham níveis significativos de bolor de levedura. Não houve diferença entre os grupos em termos microbiológicos na análise estatística (P > 0,05). De acordo com o estudo atual, não houve produção padrão e não foram observadas regras de higiene suficientes no processo de produção de pestil em empresas familiares em Elazig. Por outro lado, foram obtidas informações essenciais sobre alguns parâmetros físicos, químicos e microbiológicos de qualidade das polpas de uva e amora-preta comercializadas em mercados e lojas de Elazig. RESUMO couros (couros Elazig tanto 4 frutas 2 físicas microbiológicas L claridade/escuridão claridadeescuridão claridade escuridão (claridade/escuridão 4272 42 72 42.7 4481 44 81 44.81 vermelhidão (vermelhidão 1029 10 29 10.2 907 9 07 9.07 b amarelo (amarelo 2090 20 90 20.9 respectivamente 162 1 62 1.6 134 34 1.34 382 3 82 3.8 475 75 4.75 como cítrico 063 0 63 0.6 022 0.22% 0602 602 0,60 0627 627 0.627 6878 68 78 68.78 7299 99 72.99% 225 25 2.25 064 64 0.64% 020 0.2 029 0.29 1883 18 83 18.8 1936 19 36 19.36 TMAB coliformes StaphylococcusMicrococcus Staphylococcus Micrococcus 472 4.7 448 48 4.48 130 30 1.3 189 89 1.89 211 11 2.1 212 12 204 04 2.0 log log1 ufcg ufc g ufc/g examinadas 31.11%, 3111 31.11% , 31 (31.11%) 5 spp entanto verificouse verificou se P 0,05. 005 0,05 . 05 0,05) atual lado físicos amorapreta amora preta 427 7 42. 8 44.8 102 10. 9.0 209 20. 16 6 1. 13 38 3. 47 06 0. 02 0.22 060 60 0,6 062 0.62 687 68.7 729 72.99 2.2 0.64 188 18. 193 19.3 4. 4.4 1.8 21 2. 311 31.11 (31.11% 00 0,0 44. 9. 0, 68. 72.9 19. 31.1 (31.11 72. 31. (31.1 (31. (31 (3 (

Abstract: Bacteremia is a major cause of morbidity and mortality in patients with cancer and episodes of high-risk febrile neutropenia (HRFN). Objective: To identify the frequency of microorganisms isolated from blood cultures (BC) and their antimicrobial resistance (R) profile in children with HRFN, compared with the same data from previous studies of the same group. Method: Prospective, multicenter, epidemiological surveillance study of microorganisms isolated from BC in patients under 18 years of age, from 7 PINDA network hospitals, between 2016 and 2021. Results: 284 episodes of HRFN with positive BC were analyzed out of 1091 enrolled episodes (26%). Median age 7.2 years [3.0-12.3]. The main isolates were gram-negative bacilli (GNB) 49.2%, gram-positive cocci (GPC) 43.8%, and fungi 3.6%. The most frequently isolated microorganisms were viridans group Streptococci (VGS) (25.8%), Escherichia coli (19.8%), Pseudomonas spp. (11.2%), Klebsiella spp. (10.9%), and coagulase negative Staphylococci (CoNS) (10.9%). There was an increase in R to third-generation cephalosporins (p = 0.011) in GNB and to oxacillin in CoNS (p = 0.00), as well as a decrease in R to amikacin in non-fermenting GNB (p = 0.02) and to penicillin in VGS (p = 0.04). Conclusion: VGS is the main agent isolated in BC from pediatric patients with cancer and episodes of HRFN, followed by E. coli, Pseudomonas spp., and Klebsiella spp. Having epidemiological surveillance of microorganisms isolated from BC and their antimicrobial R profile is essential to favor the rational use of antimicrobials.

Resumen: Las bacteriemias son una causa importante de morbimortalidad en pacientes con cáncer y episodios de neutropenia febril de alto riesgo (NFAR). Objetivo: Determinar la frecuencia de microorganismos aislados de hemocultivos (HC) y su perfil de resistencia (R) antimicrobiana en niños con NFAR, comparado con iguales datos de estudios previos del mismo grupo. Material y Método: Estudio prospectivo, multicéntrico, de vigilancia epidemiológica de microorganismos aislados de HC en pacientes menores de 18 años, en siete hospitales de la red PINDA, 2016-2021. Resultados: Se analizaron 284 episodios de NFAR con HC positivos de 1091 episodios enrolados (26%). Mediana de edad 7,2 años [3,0-12,3]. Los aislados principales fueron bacilos gramnegativos (BGN) 49,2%, cocáceas grampositivas (CGP) 43,8% y hongos 3,6%, con mayor frecuencia de Streptococcus grupo viridans (SGV) (25,8%), Escherichia coli (19,8%), Pseudomonas spp. (11,2%), Klebsiella spp. (10,9%) y Staphylococcus coagulasa negativa (SCN) (10,9%). Hubo aumento de R a cefalosporinas de tercera generación en enterobacterales (p = 0,011) y de R a oxacilina en SCN (p = 0,00), así como disminución de R a amikacina en BGN no fermentadores (p = 0,02) y de R a penicilina en SGV (p = 0,04). Conclusión: SGV es el principal agente aislado en HC de pacientes pediátricos con cáncer y episodios de NF, seguido de E. coli, Pseudomonas spp. y Klebsiella spp. Contar con vigilancia epidemiológica de microorganismos aislados de HC y su perfil de R antimicrobiana es esencial para favorecer el uso racional de antimicrobianos.

Abstract This study analyzed the impact of leachate from a temporary landfill on soil microbial diversity in Tingo María, Huánuco region, Peru. Three treatments were used: untreated soil (S), addition of stream water (T0), leachate (T1), and leachate treated by coagulation and flocculation (T2), with 828.5 ml/week added in three weekly doses. Soil samples were collected from the Reserved Forest of the Universidad Nacional Agraria de la Selva. Twenty-one randomly distributed soil samples were taken and homogenized for analysis. Soil quality parameters measured included sand, clay, silt, texture, pH, organic matter, nitrogen, phosphorus and potassium. As for microorganisms, viable aerobes, lactobacilli, actinomycetes, fungi, nitrogen-fixing bacteria, and Escherichia coli were quantified using specific culture and counting methods for each of them. To evaluate the impact of the leachate on microbial diversity, equity indices (Shannon and inverse Simpson), dominance indices (complementary Simpson and Berger Parker) and the percentage composition of each microorganism per treatment were used. An ANOVA was performed to estimate differences in microbial diversity, with a Tukey test at a significance level of α = 0.05. The study showed that leachates affect soil microbial diversity, reducing equity and increasing the dominance of certain species such as E. coli. They also alter physicochemical parameters, decreasing organic matter and nitrogen but increasing other elements such as phosphorus and potassium. This could have implications for soil health and functionality.

Abstract In Argentina, hemolytic uremic syndrome (HUS) caused by EHEC has the highest incidence in the world. EHEC infection has an endemo-epidemic behavior, causing 20-30% of acute bloody diarrhea syndrome in children under 5 years old. In the period 2016-2020, 272 new cases per year were notified to the National Health Surveillance System. Multiple factors are responsible for HUS incidence in Argentina including person-to-person transmission. In order to detect possible EHEC carriers, we carried out a preliminary study of the frequency of kindergarten teachers with anti-LPS antibodies against the most prevalent EHEC serotypes in Argentina. We analyzed 61 kindergarten teachers from 26 institutions from José C. Paz district, located in the suburban area of Buenos Aires province, Argentina. Fifty-one percent of the plasma samples had antibodies against O157, O145, O121 and O103 LPS: 6.4% of the positive samples had IgM isotype (n=2), 61.3% IgG isotype (n=19) and 32.3% IgM and IgG (n=10). Given that antibodies against LPS antigens are usually short-lived specific IgM detection may indicate a recent infection. In addition, the high percentage of positive samples may indicate a frequent exposure to EHEC strains in the cohort studied, as well as the existence of a large non-symptomatic population of adults carrying pathogenic strains that could contribute to the endemic behavior through person-to-person transmission. The improvement of continuous educational programs in kindergarten institutions could be a mandatory measure to reduce HUS cases not only in Argentina but also globally.

Resumen En Argentina, el síndrome urémico hemolítico causado por Escherichia coli enterohemorrágica (EHEC) tiene la más alta incidencia del mundo. Las infecciones por EHEC tienen un comportamiento endemoepidémico y causan del 20 al 30% de los síndromes de diarrea sanguinolenta en niños menores de 5 años. En el período 2016-2020, se notificaron 272 nuevos casos por año al Sistema de Vigilancia de Salud Nacional. Múltiples factores son responsables de la alta incidencia de SUH en Argentina, incluyendo la transmisión persona-persona. Con el objetivo de detectar posibles portadores asintomáticos de EHEC, realizamos un estudio preliminar de la frecuencia de anticuerpos antilipopolisacáridos contra los serotipos de EHEC más prevalentes en Argentina. El estudio se realizó con muestras de plasma obtenidas de 61 maestras y maestros de jardines de infantes de 26 instituciones del distrito de José C. Paz, localizado en el área suburbana de la provincia de Buenos Aires, Argentina. El 51% de las muestras presentaron anticuerpos contra los serotipos de lipopolisacáridos O157, O145, O121 y O103; el 6,4% de las muestras positivas tuvieron el isotipo IgM (n=2), el 61,3% el isotipo IgG (n=19) y el 32,3% los isotipos IgM e IgG (n=10). Dado que los anticuerpos antilipopolisacáridos presentan usualmente una duración corta, la detección de IgM específica podría indicar una infección reciente. Además, el alto porcentaje de muestras positivas hallado podría indicar una exposición frecuente a las cepas de EHEC en la cohorte estudiada. Asimismo, la gran población de adultos portadores asintomáticos de estas cepas patógenas podría contribuir al comportamiento endémico, a través de la transmisión persona-persona. El perfeccionamiento de programas educacionales continuos en jardines de infantes podría constituir una medida importante para reducir los casos de síndrome urémico hemolítico, no solo en Argentina, sino también en el mundo.

Abstract Diarrheagenic Escherichia coli comprises a heterogeneous group of pathotypes or pathogenic variants that share phenotypic characteristics with marked differences in virulence genes, colonization sites, pathogenesis, clinical presentation, and epidemiology of infection. The most studied pathotypes are Shiga toxin-producing E.coli (STEC), enterotoxigenic E.coli (ETEC), enteropathogenic E.coli (EPEC), enteroaggregative E.coli (EAEC), and enteroinvasive E.coli (EIEC). The objective of the study was to characterize the isolates of diarrheagenic E.coli from an outpatient pediatric population with diarrhea attended in two public hospitals from Buenos Aires, Argentina. Diarrheagenic E.coli pathotypes were investigated by amplifying characteristic virulence gene fragments: intimin (eae), heat-labile toxin (lt), heat-stable toxins (stp, sth), invasion plasmid antigen H (ipaH), transcriptional activator R (aggR) and Shiga toxins (stx1, stx2). Molecular subtyping of isolates was performed using PFGE (XbaI). Diarrheagenic E.coli was detected in 14% (84/601) of cases. The EAEC pathotype was prevalent, while ETEC, STEC, EPEC and EIEC were found in a lower proportion. EAEC isolates exhibited a high degree of genetic diversity. All pathotypes were found in children under 5years of age, while only EAEC, EIEC and ETEC were detected in the older population. Future studies that include the characterization of isolates from a greater number of genes and populations from other geographical areas will be necessary to determine the relevance of diarrheagenic E.coli in Argentina.

Resumen Escherichia coli diarreogénica abarca un grupo heterogéneo de patotipos o variantes patogénicas que comparten características fenotípicas con marcadas diferencias en genes de virulencia, sitios de colonización, patogenia, presentación clínica y epidemiología de la infección. Los patotipos más estudiados son E.coli productora de toxina Shiga (STEC), E.coli enterotoxigénica (ETEC), E.coli enteropatogénica (EPEC), E.coli enteroagregativa (EAEC) y E.coli enteroinvasiva (EIEC). El objetivo del estudio fue caracterizar los aislamientos de E.coli diarreogénica provenientes de población pediátrica ambulatoria con diarrea, atendida en dos hospitales públicos de Buenos Aires, Argentina. Los patotipos de E.coli diarreogénica se investigaron mediante la amplificación de fragmentos de genes de virulencia característicos: intimina (eae), toxina termolábil (lt), toxinas termoestables (stp, sth), antígeno plasmídico de invasiónH (ipaH), activador transcripcional R (aggR) y toxinas Shiga (stx1, stx2). La subtipificación molecular de aislamientos se realizó mediante PFGE (XbaI). E.coli diarreogénica fue detectada en el 14% (84/601) de los casos. El patotipo EAEC fue prevalente, mientras que ETEC, STEC, EPEC y EIEC fueron hallados en menor proporción. Los aislamientos de EAEC presentaron un alto grado de diversidad genética. Todos los patotipos fueron hallados en niños menores de 5años, mientras que solamente EAEC, EIEC y ETEC fueron detectados en población de mayor edad. Futuros estudios que incluyan la caracterización de aislamientos a partir de un mayor número de genes y población de otras áreas geográficas serán necesarios para determinar la relevancia de E.coli diarreogénica en Argentina.

Biologically active compounds in most spices possess antimicrobial and other important biomedical properties. There have been huge demands for natural immunity boosters (spices and herbs), considering the recent global pandemic and challenges relating to drug-resistant pathogens. This study was designed to compare the efficacy of ginger, garlic and turmeric spices against some pathogenic microorganisms. Aqueous extraction of spices, antimicrobial sensitivity and minimum inhibitory concentration tests were done using standard microbiological methods. Bioactive compounds were estimated using the gas chromatography-mass spectrometry (GC-MS) method. Aqueous extracts of ginger inhibited the growth of all test isolates except Streptococcus pneumoniae, with inhibition zones ranging between 0.9 mm and 13.5 mm. Escherichia coli, S. pneumoniae and Haemophilus influenzae were resistant to turmeric extracts, while the extract of garlic inhibited only four of the test pathogens. Inhibition zones for turmeric ranged between 4.4 mm and 10.9 mm, while those for garlic were between 4.7 mm and 11.5 mm. All the spice extracts did not inhibit microbial growth at 10-40%. An antibiotic spectrum indicated that Bacillus sp. was resistant to all but one, nitrofurantoin, which also inhibited the growth of almost all pathogens, except H. influenzae, with zones ranging between 10.5 mm and 11.6 mm. All test pathogens were resistant to cloxacillin except E. coli (10.6 mm). The major phyto-active compounds present in ginger are 2-Butanone,4-(4-hydroxy-3-methoxyphenyl), 1,3-Cyclohexadiene and 1-(4-Hydroxy-3-methoxyphenyl). SIGNIFICANCE: Conclusively, ginger, turmeric and garlic have varied inhibitory activities against diverse organisms, indicating their antimicrobial properties; however, ginger showed a higher inhibitory effect and more diverse antimicrobial property amongst selected isolates. Furthermore, certain bioactive compounds of biomedical importance were present. We therefore recommend the use of these spices as alternative natural food preservatives against spoilage organisms, as well as potential natural sources for bioactive compounds in drug development against pathogens.

FtsZ is a bacterial divisome protein responsible for Z-ring formation in cytokinesis. Characterization of Escherichia coli FtsZ protein oligomers (EcFtsZ) in native conditions is defiance because the protein is found as a multi-oligomer in self-association-dissociation equilibrium. We characterize the trimeric state of EcFtsZ through native PAGE, gel filtration chromatography and sucrose gradient techniques combined with chemical cross-linking. The filtration results indicate that the EcFtsZ trimer has a molecular mass of 131 kDa and a filtration friction coefficient (Rs/Rmin) equal to 1.9, while the theoretical filtration friction coefficient (fn/f1) calculated for a linear trimer yielded a value equal to 1.8 very close to the experimental value. On the other side, formaldehyde-crosslinked EcFtsZ showed a band 128 kDa recognized by anti-FtsZ antibodies, and a sedimentation friction coefficient (Smax/S20,w) equal to 1.9, while the theoretical sedimentation friction coefficient (Sn/S1) calculated for a triangular trimer was equal to the experimental value. These results suggest that EcFtsZ has two homotrimeric structures (linear and triangular). Finally, we report the aggregation of EcFtsZ at micromolar concentrations of Capsaicin without GTP and Mg2+.

La FtsZ es una proteína del divisoma bacteriano responsable de la formación del anillo Z en la citocinesis. La caracterización de los oligómeros de la proteína FtsZ de Escherichia coli (EcFtsZ) en su estado nativo es un desafío porque la proteína se encuentra como un multioligómero en equilibrio de autoasociación-disociación. Nosotros caracterizamos el estado trimérico de la EcFtsZ a través de técnicas de PAGE nativo, cromatografía de filtración en gel y gradiente de sacarosa combinada con entrecruzamiento químico. Los resultados por filtración indican que el trímero de EcFtsZ tiene una masa molecular de 131 kDa y un coeficiente de fricción por filtración (Rs/Rmin) igual a 1.9, mientras que el coeficiente de fricción por filtración teórico (fn/f1) calculado para un trímero lineal arrojó un valor igual a 1.8 que está muy cercano al valor experimental. Por otro lado, la EcFtsZ entrecruzada con formaldehído presentó una banda de 128 kDa reconocida por anticuerpos anti-FtsZ y un coeficiente de fricción por sedimentación (Smax/S20,w) igual a 1.9, mientras que el coeficiente de fricción por sedimentación teórico (Sn/S1) calculado para un trímero triangular resultó igual a valor experimental. Estos resultados sugieren que la EcFtsZ tiene dos estructuras homotriméricas (lineal y triangular). Finalmente, reportamos la agregación de la EcFtsZ a concentraciones micromolares de Capsaicina sin GTP y Mg2+.

Resumo O peixe é a principal fonte de proteína animal para a alimentação humana. O objetivo deste estudo foi descobrir a prevalência de bactérias patogênicas de dois peixes economicamente importantes selecionados do Paquistão, nomeadamente Mahseer (Tor putitora) e carpa prateada (Hypophthalmichthys molitrix). Amostras de peixes vivos de incubatórios e amostras de peixes mortos de diferentes mercados da área de estudo foram coletadas aleatoriamente. As amostras de peixes foram analisadas quanto ao isolamento, identificação e prevalência de bactérias. As bactérias isoladas dos peixes do estudo foram identificadas através de testes bioquímicos e cerca de 10 espécies de bactérias patogênicas foram identificadas incluindo as bactérias patogênicas para humanos e peixes, nomeadamente, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus iniae, Serratia spp. Citrobacter spp. Stenotrophomonas spp. Bacillus spp. e Salmonella spp. A porcentagem de freqüência de ocorrência bacteriana em carpa prateada e peixes Mahseer mostrou Pseudomonas aeruginosa 21,42%, Staphylococcus epidermidis 17,85%, Escherichia coli 11,90%, Staphylococcus aureus 9,52%, Citrobacter spp. 9,52%, Serratia spp. 8,33%, Streptococcus iniae 7,14%, Stenotrophomonas spp. 5,95%, Bacillus spp. 4,76% e Salmonella spp. 3,57%. O estudo revelou que as amostras de peixes de Mahseer e carpa prateada coletadas nos mercados encontraram mais isolados (10 espécies bacterianas) do que as amostras de peixes frescos (03 espécies bacterianas) de incubatórios. A ocorrência de bactérias patogênicas nos peixes do estudo apresentou fator de risco para consumidores de saúde pública.

Abstract Fish is the main source of animal protein for human diet. The aim of this study was to find out prevalence of pathogenic bacteria of two selected economically important fish of Pakistan namely Mahseer (Tor putitora) and Silver carp (Hypophthalmichthys molitrix). Live fish samples from hatcheries and dead fish samples from different markets of study area were randomly collected. The fish samples were analyzed for isolation, identification and prevalence of bacteria. The isolated bacteria from study fish were identified through biochemical test and about 10 species of pathogenic bacteria were identified including the pathogenic bacteria to human and fish namely, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus iniae, Serratia spp. Citrobacter spp. Stenotrophomonas spp. Bacillus spp. and Salmonella spp. The bacterial percentage frequency of occurrence in Silver carp and Mahseer fish showed Pseudomonas aeruginosa 21.42%, Staphylococcus epidermidis 17.85%, Escherichia coli 11.90%, Staphylococcus aureus 9.52%, Citrobacter spp. 9.52%, Serratia spp. 8.33%, Streptococcus iniae 7.14%, Stenotrophomonas spp. 5.95%, Bacillus spp. 4.76% and Salmonella spp. 3.57%. The study revealed that Fish samples of Mahseer and Silver carp that were collected from markets have found more isolates (10 bacterial species) than did the fresh fish pond samples (03 bacterial species) of hatcheries. The occurrence of pathogenic bacteria in study fish showed risk factor for public health consumers.

Resumo O presente estudo foi realizado para isolar e caracterizar bactérias de amostras de água e solo retiradas do Canal Lahore, em Lahore, em diferentes locais, ou seja, Mall Road, Mohlanwal e Khera. As cepas bacterianas isoladas foram identificadas com base em testes morfológicos e bioquímicos. A identificação foi confirmada por cultura de bactérias em testes de meios seletivos. O teste de resistência aos antibióticos também foi realizado para observar a resistência das bactérias a diferentes antibióticos. Foi realizado o teste de ágar sangue para identificar diferentes bactérias patogênicas. O resultado revelou que amostras de água e solo do Canal Lahore, Lahore, de diferentes localidades estavam contaminadas com Escherichia coli, Salmonella sp., Vibrio sp., Bacillus spp., Enterococcus sp. e Staphylococcus spp. Por causa da presença desses patógenos, essa água não é adequada para qualquer uso doméstico e de irrigação. O estudo revelou que a água do Canal Lahore é prejudicial à saúde humana, pois está contaminada com bactérias que podem causar doenças graves, por exemplo: Escherichia coli pode ocasionar gastroenterite; Bacillus spp. pode causar náuseas e vômitos; Enterococcus sp. pode infectar o trato urinário; Salmonella sp. é responsável pela bacteremia; Staphylococcus spp. pode causar febre leve; e Vibrio sp. pode ser a razão da cólera. Assim, torna-se imprópria para uso humano, como natação, banho, lavagem etc., até que algumas medidas corretivas sejam empregadas para erradicar microrganismos patogênicos por WASA e LWMS de acordo com os padrões da OMS. Da mesma forma, é bastante prejudicial, quando usada para irrigação sem tratamento adequado.

Abstract The present study was conducted to isolate and characterize bacteria from water and soil sample taken from the Lahore Canal at different sites i.e. Mall Road, Mohlanwal and Khera site. Isolated bacterial strains were identified on the basis of morphological and biochemical tests. Identification was confirmed by culturing bacteria on selective media. Antibiotic resistance test was also performed to observe the resistance of bacteria against different antibiotics. Blood agar test was performed for identification of different pathogenic bacteria. The result revealed that water and soil samples of Lahore Canal Lahore from different sites were contaminated with Escherichia coli, Salmonella sp., Vibrio sp., Bacillus spp., Enterococcus sp. and Staphylococcus spp. Due to presence of these pathogens, this water is not suitable for any domestic and irrigation use. Study also revealed that water of the Lahore Canal is harmful for human health as it is contaminated with bacteria that can cause severe disease e.g., Escherichia coli can cause gastroenteritis, Bacillus spp. can cause nausea and vomiting, Enterococcus may infect urinary tract, Salmonella sp. is responsible for Bacteremia, Staphylococcus spp. can cause mild fever and Vibrio sp. can be the reason of cholera. Thus it is rendered unfit for any kind of human use even other than drinking like swimming, bathing, washing etc., until and unless some remedial measures are employed to eradicate pathogenic microorganisms by WASA and LWMS according to standards of WHO. Similarly, it is quite harmful, when and where ever it is used for irrigation without proper treatment.

Resumo A microbiota gastrointestinal regula as funções do corpo e desempenha um papel importante na sua saúde. A disbiose leva a uma série de doenças crônicas, como diabetes, obesidade, inflamação, aterosclerose, etc. No entanto, essas doenças podem ser prevenidas pelo uso de probióticos − microrganismos vivos que beneficiam a microflora e, portanto, melhoram a saúde do organismo hospedeiro. Os probióticos mais comuns incluem bactérias do ácido láctico dos gêneros Bifidobacterium e Propionibacterium. Nós estudamos as propriedades probióticas das seguintes cepas: Bifidobacterium adolescentis АС-1909, Bifidobacterium longum infantis АС-1912, Propionibacterium jensenii В-6085, Propionibacterium freudenreichii В-11921, Propionibacterium thoenii В-6082 В-6082 acid e Propionibacterium thoenii В-6082 В-6082 acidibion. A atividade antimicrobiana foi determinada pelo método de 'blocos de ágar' contra as seguintes culturas de teste: Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa B6643, Proteus vulgaris ATCC 63 e Listeria monocytogenes moderada atividade ATCC 7644. Uma atividade antimicrobiana moderada contra todas as culturas de teste foi registrado em Bifidobacterium adolescentis АС-1909, Propionibacterium jensenii В-6085 e Propionibacterium thoenii В-6082. A atividade antioxidante foi determinada pelo método de inibição do DPPH em todas as cepas de ácido lático. Nosso estudo indicou que algumas cepas de Propionibacterium e Bifidobacterium − ou, teoricamente, seus consórcios − poderiam ser usadas como culturas probióticas em suplementos dietéticos ou alimentos funcionais para prevenir uma série de doenças crônicas.

Abstract The gastrointestinal microflora regulates the body’s functions and plays an important role in its health. Dysbiosis leads to a number of chronic diseases such as diabetes, obesity, inflammation, atherosclerosis, etc. However, these diseases can be prevented by using probiotics – living microorganisms that benefit the microflora and, therefore, improve the host organism's health. The most common probiotics include lactic acid bacteria of the Bifidobacterium and Propionibacterium genera. We studied the probiotic properties of the following strains: Bifidobacterium adolescentis АС-1909, Bifidobacterium longum infantis АС-1912, Propionibacterium jensenii В-6085, Propionibacterium freudenreichii В-11921, Propionibacterium thoenii В-6082, and Propionibacterium acidipropionici В-5723. Antimicrobial activity was determined by the ‘agar blocks’ method against the following test cultures: Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa B6643, Proteus vulgaris ATCC 63, and Listeria monocytogenes ATCC 7644. Moderate antimicrobial activity against all the test cultures was registered in Bifidobacterium adolescentis АС-1909, Propionibacterium jensenii В-6085, and Propionibacterium thoenii В-6082. Antioxidant activity was determined by the DPPH inhibition method in all the lactic acid strains. Our study indicated that some Propionibacterium and Bifidobacterium strains or, theoretically, their consortia could be used as probiotic cultures in dietary supplements or functional foods to prevent a number of chronic diseases.