ABSTRACT Cuminum cyminum L. (CM), Zataria multiflora Boiss. (ZM) and Mentha piperita L. (MP) are traditional medicinal plants with various pharmacological properties. This study was designed to assess the role of gamma irradiation -a modern decontamination method- in hepatoprotective effects of their essential oil (E.Os) in septic rats induced by experimental cecal ligation and puncture (CLP) model. The rats were divided into 20 groups; sham-operated (SOP); CLP; CLP + CM, ZM and MP (E.Os) (100 & 200 mg/kg b.w) and CLP + gamma irradiated (10 and 25 kGy) E.Os (100 & 200 mg/kg b.w) as treatment groups. All E.Os were injected i.p immediately after sepsis induction. 24 hour after CLP, the rats were sacrificed and the liver tissue was examined considering lipid peroxiation (LP), glutathione (GSH) and myeloperoxidase (MPO) activity. The results indicated that CLP operation caused significant (P<0.05) increase in the LP and MPO levels concomitant with decreased GSH level. Administration of the E.Os (100 and 200 mg/kg b.w) extracted from non irradiated plants as well as the irradiated (10 and 25 kGy) plant E.Os could significantly (P<0.05) modulate the levels of LP, MPO and GSH. It can be concluded that all E.Os even after irradiation exposure could modulate the oxidative injury parameters related to liver damages in CLP rat model. In conclusion, the plant irradiation didn’t have any adverse effects on the hepatoprotective activities of the extracted oils.