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Abstract The aim of this study was to evaluate the influence of diluent, stored volume and the cryodiluent medium supplementation with sulfated polysaccharides (SP) extracted from Nile tilapia skin on P. brevis vitrified sperm. Six pools were diluted in 5% Glucose or Powder Coconut Water (ACP-104), supplemented or not with 0.5 mg/mL of SP, and submitted to vitrification. Subsequently, they were stored in cryotubes in two volumes (60 μL and 420 μL). After 15 days, the samples were devitrified and evaluated for kinetics, membrane integrity and sperm DNA integrity. ACP-104 proved to be the best diluent for P. brevis sperm vitrification. Membrane and DNA integrity rates were higher (P < 0.05) when stored in smaller and larger volume, respectively. Additionally, the best rates (P < 0.05) of these same parameters were obtained with supplemented medium. There was interaction (P < 0.05) between diluent and stored volume, with ACP-104 exceeding 5% Glucose for motility in both volumes, while for average path speed (VAP) and membrane integrity the same happened in the larger volume. 5% Glucose had higher VAP and membrane integrity when stored in smaller volume. There was a triple interaction (P < 0.05) for DNA integrity, and better results were obtained when semen was vitrified in ACP-104 and stored in the larger volume, regardless of supplementation, which influenced only the 5% Glucose medium in the smaller volume. It was concluded that ACP-104, supplemented with SP and stored in larger volume make up the best treatment for P. brevis sperm vitrification.

https://doi.org/10.1590/1984-3143-ar2024-0075

NURSING TECHNICIANS' PROXEMIC BEHAVIORS DURING TRANSFUSIONS IN HOSPITALIZED CLIENTS
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Gonçalves, Francisco Gleidson de Azevedo

; Araújo, Sílvia Teresa Carvalho de

; Azevedo, Albert Lengruber de

; Ferreira, Priscila Brigolini Porfírio

; Torres, Danelia Gómez

; Pérez Júnior, Eugenio Fuentes

; Bastos, Soraia do Socorro Furtado

; Silva, Suely Francisco da

Texto & Contexto - Enfermagem

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2024, Volume 33 elocation e20230207

Abstract: Es Pt En | Text: Es Pt En | PDF: Es | PDF: Pt | PDF: En

RESUMEN Objetivo: describir los comportamientos proxémicos de técnicos de Enfermería durante transfusiones en clientes internados. Método: estudio cualitativo, descriptivo y exploratorio, basado en la observación no participante y registrado en un instrumento elaborado a partir de la Teoría del Comportamiento Proxémico. Los participantes fueron 18 técnicos de Enfermería de un hospital que es referencia en Hematología y Hemoterapia de la Región Sudeste de Brasil, entre abril y diciembre de 2022. En el análisis de los datos se priorizó el contenido temático. Resultados: se destacó lo siguiente: contacto visual; toque instrumental; tono de voz normal; y distancias íntima y personal; los cuales indican buen recibimiento, atención y vigilancia técnica al realizar las transfusiones. Conclusión: es necesario generar conciencia en los técnicos de Enfermería con respecto a los comportamientos proxémicos al recibir a los clientes. Eso implica: prestar más atención al escuchar y ampliar los vínculos y la convivencia interpersonal efectiva, además de favorecer el alcance de las medidas de atención en integrales en Hematología y Hemoterapia.

RESUMO Objetivo: descrever os comportamentos proxêmicos de técnicos de enfermagem durante o ato transfusional em clientes hospitalizados. Método: estudo qualitativo, descritivo e exploratório, pautado em observação não participante, registrado em instrumento elaborado a partir da teoria do comportamento proxêmico. Participaram 18 técnicos de enfermagem de um hospital referência em hematologia e hemoterapia da Região Sudeste do Brasil, de abril a dezembro de 2022. A análise de dados priorizou o conteúdo temático. Resultados: destacaram-se o contato visual; o toque instrumental; o tom de voz normal e as distâncias íntima e pessoal. Estes, indicativos de acolhimento, atenção e vigilância técnica à realização do ato transfusional. Conclusão: há necessidade de despertar a consciência dos técnicos de enfermagem em relação aos comportamentos proxêmicos durante o acolhimento aos clientes. Isto é: ampliar a escuta, o vínculo, a efetiva convivência interpessoal e favorecer o alcance dos cuidados em saúde integral na hematologia e hemoterapia.

ABSTRACT Objective: to describe nursing technicians' proxemic behaviors during transfusions in hospitalized clients. Method: a qualitative, descriptive and exploratory study based on non-participant observation, recorded in an instrument prepared from the Theory of Proxemic Behavior. The participants were 18 nursing technicians from a reference hospital for Hematology and Hemotherapy in the Brazilian Southeast Region, from April to December 2022. Data analysis prioritized thematic content. Results: the following stood out: eye contact; instrumental touch; normal tone of voice; and intimate and personal distances. These indicate welcoming, attention and technical vigilance when carrying out the transfusions. Conclusion: there is a need to raise nursing technicians' awareness regarding proxemic behaviors when welcoming clients. That is: expanding listening, bonding and effective interpersonal coexistence, and favoring the reach of comprehensive health care in Hematology and Hemotherapy.

https://doi.org/10.1590/1980-265x-tce-2023-0207en

Safety of CoronaVac and ChAdOx1 vaccines against SARS-CoV-2 in patients with rheumatoid arthritis: data from the Brazilian multicentric study safer
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Cruz, Vitor Alves

; Guimarães, Camila Rêgo, Jozelia Machado, Ketty Lysie Libardi Lira Miyamoto, Samira Tatiyama Burian, Ana Paula Neves Dias, Laiza Hombre Pretti, Flavia Zon Batista, Danielle Cristina Filgueira Alves Mill, José Geraldo Oliveira, Yasmin Gurtler Pinheiro de Gadelha, Carolina Strauss Estevez Gouveia, Maria da Penha Gomes Moulin, Anna Carolina Simões Souza, Bárbara Oliveira Aguiar, Laura Gonçalves Rodrigues Vieira, Gabriel Smith Sobral Grillo, Luiza Lorenzoni Lima, Marina Deorce de Pasti, Laís Pizzol Surlo, Heitor Filipe Faé, Filipe Moulaz, Isac Ribeiro Macabú, Mariana de Oliveira Ribeiro, Priscila Dias Cardoso Magalhães, Vanessa de Oliveira Aguiar, Mariana Freitas de Biegelmeyer, Erika Peixoto;, Flávia Maria Matos Melo Campos Kayser, Cristiane Souza, Alexandre Wagner Silva de Castro, Charlles Heldan de Moura Ribeiro, Sandra Lúcia Euzébio Telles, Camila Maria Paiva França Bühring, Juliana Lima, Raquel Lima de Santos, Sérgio Henrique Oliveira Dos Dias, Samuel Elias Basualto Melo, Natália Seixas de Sanches, Rosely Holanda da Silva Boechat, Antonio Luiz Sartori, Natália Sarzi Hax, Vanessa Dória, Lucas Denardi Rezende, Rodrigo Poubel Vieira de Baptista, Katia Lino Fortes, Natália Rodrigues Querido Melo, Ana Karla Guedes de Melo, Tâmara Santos Vieira, Rejane Maria Rodrigues de Abreu Vieira, Adah Sophia Rodrigues Kakehasi, Adriana Maria Tavares, Anna Carolina Faria Moreira Gomes Landa, Aline Teixeira de Costa, Pollyana Vitoria Thomaz da Azevedo, Valderilio Feijó Martins-Filho, Olindo Assis Peruhype-Magalhães, Vanessa Pinheiro, Marcelo de Medeiros Monticielo, Odirlei André Reis-neto, Edgard Torres Dos Ferreira, Gilda Aparecida Souza, Viviane Angelina de Teixeira-Carvalho, Andréa Xavier, Ricardo Machado Sato, Emilia Inoue Valim, Valeria Pileggi, Gecilmara Salviato Silva, Nilzio Antonio da

Advances in Rheumatology

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2024, Volume 64 elocation 58

Abstract: En | Text: En | PDF: En

Abstract Background Patients with immune-mediated rheumatic diseases (IMRDs) have been prioritized for COVID-19 vaccination to mitigate the infection severity risks. Patients with rheumatoid arthritis (RA) are at a high risk of severe COVID-19 outcomes, especially those under immunosuppression or with associated comorbidities. However, few studies have assessed the safety of the COVID-19 vaccine in patients with RA. Objective To evaluate the safety of vaccines against SARS-CoV-2 in patients with RA. Methods This data are from the study “Safety and Efficacy on COVID-19 Vaccine in Rheumatic Diseases,” a Brazilian multicentric prospective phase IV study to evaluate COVID-19 vaccine in IMRDs in Brazil. Adverse events (AEs) in patients with RA of all centers were assessed after two doses of ChAdOx1 (Oxford/AstraZeneca) or CoronaVac (Sinovac/Butantan). Stratification of postvaccination AEs was performed using a diary, filled out daily and returned at the end of 28 days for each dose. Results A total of 188 patients with RA were include, 90% female. CoronaVac was used in 109 patients and ChAdOx1 in 79. Only mild AEs were observed, mainly after the first dose. The most common AEs after the first dose were pain at the injection (46,7%), headache (39,4%), arthralgia (39,4%), myalgia (30,5%) and fatigue (26,6%), and ChAdOx1 had a higher frequency of pain at the injection (66% vs 32 %, p < 0.001) arthralgia (62% vs 22%, p < 0.001) and myalgia (45% vs 20%, p < 0.001) compared to CoronaVac. The more common AEs after the second dose were pain at the injection (37%), arthralgia (31%), myalgia (23%), headache (21%) and fatigue (18%). Arthralgia (41,4% vs 25%, p = 0.02) and pain at injection (51,4% vs 27%, p = 0.001) were more common with ChAdOx1. No serious AEs were related. With Regard to RA activity level, no significant difference was observed between the three time periods for both COVID-19 vaccines. Conclusion In the comparison between the two immunizers in patients with RA, local reactions and musculoskeletal symptoms were more frequent with ChAdOx1 than with CoronaVac, especially after the first dose. In summary, the AE occurred mainly after the first dose, and were mild, like previous data from others immunizing agents in patients with rheumatoid arthritis. Vaccination did not worsen the degree of disease activity.

https://doi.org/10.1186/s42358-024-00397-5

Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses
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Koltun, Alessandra

; Silva, Priscila Alves da Torres, Isabela Yamauchi Bonifácio-Anacleto, Fernando Yassitepe, Juliana Erika de Carvalho Teixeira

Crop Breeding and Applied Biotechnology

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2024, Volume 24 N. 1 elocation e46392411

Abstract: En | Text: En | PDF: En

Abstract Microsatellites have been widely used to genotype individuals and to address a myriad of biological questions in many research fields for decades. However, when implementing a microsatellite marker analysis routine from scratch, various problems can arise throughout the process from DNA extraction to allele scoring, including inputting errors in the database, decreasing the reliability of results and having profound negative impacts on the derived decisions. Therefore, correctly assigning a genotype to a sample is crucial and dependent on acquiring knowledge of the technique steps, such as the chemistry of reactions, software, and data curation. This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.

https://doi.org/10.1590/1984-70332024v24n1a01

Metabolic and ultrastructural renal changes in adult Wistar rats fed by a cafeteria diet
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Santos, Priscila Fernandes dos

; Souza, Diogo Benchimol de

; Torres, Eduardo José Lopes

; Costa, Waldemar Silva

; Sampaio, Francisco José Barcellos

; Gregorio, Bianca Martins

Acta Cirúrgica Brasileira

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2024, Volume 39 elocation e392224

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: To evaluate, by quantitative and qualitative methods, the glomerular ultrastructure in Wistar rats fed a cafeteria diet. Methods: Male Wistar rats were divided into two groups at 21 days of age: control (C, n = 10) and cafeteria diet (CAF, n = 8). The animals were followed up until 5 months of age, followed by euthanasia. The blood, kidneys, and fat deposits––epididymal, retroperitoneal, and subcutaneous––were extracted and analyzed. Data were analyzed by Student’s t test, and p < 0.05 was considered statistically significant. Results: The cafeteria diet promoted glucose intolerance, hyperglycemia (p < 0.0001), and deposition of retroperitoneal fat (p < 0.005). Scanning electron microscopy revealed that the length of the foot process was similar in both groups. The quantitative analyses by transmission electron microscopy revealed that the cafeteria diet reduced the thickness of the glomerular basement membrane (p < 0.05). Conclusions: The intake of lipids and simple carbohydrates were found to be associated with alteration in the glomerular ultrastructure. However, more studies are needed to evaluate not only the effects of high-protein and high-fat diets on components of the glomerular filtration barrier, but also renal physiology.

https://doi.org/10.1590/acb392224

Nursing team proxemic mapping in the hemotherapy space
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Gonçalves, Francisco Gleidson de Azevedo

; Araújo, Silvia Teresa Carvalho de

; Azevedo, Albert Lengruber de

; Ferreira, Priscila Brigolini Porfírio

; Torres, Danelia Gómez

; Costa, Fernanda de Nazaré Almeida

; Pires, Ariane da Silva

; Bastos, Soraia do Socorro Furtado

Revista da Escola de Enfermagem da USP

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2024, Volume 58 elocation e20240131

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: Analisar a influência dos fatores proxêmicos na comunicação e no cuidado prestado pelos profissionais de enfermagem durante o ato transfusional na hemoterapia. Método: Estudo descritivo, exploratório e qualitativo, com 25 profissionais de enfermagem de hospital especializado em doenças onco-hematológicas do Rio de Janeiro, a partir de roteiro sistematizado, registro individual dos fatores proxêmicos descritos por Edward Hall e entrevista situacional gravada. A análise considerou o conteúdo temático dos dados e utilizou o programa SketchUp 3D Modeling Software Review para demonstrar visualmente o mapeamento comportamental da interação dos profissionais de enfermagem com os clientes durante o cuidado. Resultados: As distâncias íntimas, pessoal, social e pública traduziram fatores proxêmicos, como os movimentos repetidos de ombros, pescoço e cabeça, que influenciaram positivamente o cuidado de enfermagem na hemoterapia, favorecendo a identificação das manifestações não verbais e verbais da equipe na interação com o cliente. Conclusão: A comunicação não verbal proxêmica deve ser resultante da consciência sobre disposição do espaço físico, das ações imediatas de cuidado, da pertinente e apropriada proximidade, da vigilância contínua visual e do reconhecimento manifesto das expressões traduzidas por interesse, respeito e zelo.

ABSTRACT Objective: To analyze the influence of proxemic factors on communication and care provided by nursing professionals during transfusion in hemotherapy. Method: A descriptive, exploratory and qualitative study with 25 nursing professionals from a hospital specializing in onco-hematological diseases in Rio de Janeiro, based on a systematized script, individual records of proxemic factors described by Edward Hall and recorded situational interviews. The analysis considered data thematic content and used the SketchUp 3D Modeling Software Review program to visually demonstrate the behavioral mapping of the interaction of nursing professionals with patients during care. Results: Intimate, personal, social and public distances translated into proxemic factors such as repeated movements of shoulders, neck and head, which positively influenced nursing care in hemotherapy, favoring the identification of non-verbal and verbal manifestations of the team in interaction with patients. Conclusion: Nonverbal proxemic communication must result from awareness of the layout of physical space, immediate care actions, pertinent and appropriate proximity, continuous visual surveillance and clear recognition of expressions translated as interest, respect and zeal.

RESUMEN Objetivo: Analizar la influencia de los factores proxémicos en la comunicación y el cuidado brindado por los profesionales de enfermería durante el acto transfusional en hemoterapia. Método: Estudio descriptivo, exploratorio y cualitativo, con 25 profesionales de enfermería de un hospital especializado en enfermedades oncohematológicas de Río de Janeiro, a partir de un guión sistematizado, registro individual de los factores proxémicos descritos por Edward Hall y entrevista situacional grabada. El análisis consideró el contenido temático de los datos y utilizó el programa SketchUp 3D Modeling Software Review para demostrar visualmente el mapeo conductual de la interacción de los profesionales de enfermería con los clientes durante la atención. Resultados: Las distancias íntimas, personales, sociales y públicas tradujeron factores proxémicos, como movimientos repetidos de hombros, cuello y cabeza, que influyeron positivamente en los cuidados de enfermería en hemoterapia, favoreciendo la identificación de manifestaciones verbales y no verbales del equipo en interacción con el cliente. Conclusión: La comunicación no verbal proxémica debe resultar de la conciencia de la disposición del espacio físico, de las acciones de cuidado inmediato, de la proximidad pertinente y adecuada, de la vigilancia visual continua y del reconocimiento claro de las expresiones traducidas por interés, respeto y celo.

https://doi.org/10.1590/1980-220x-reeusp-2024-0131en

Willingness and skills among students from non-health academic fields in providing efficient basic life support
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Sugimoto, Perola Nakandakari

; Gouvêa, Gabriela Buno

; Salles, Igor Caitano

; Carvalho, Heráclito Barbosa de

; Aikawa, Priscila

; Azi, Liana Maria Torres de Araújo

; Silva, Luiz Fernando Ferraz da

; Macchione, Mariangela

; Semeraro, Federico

; Lockey, Andrew

; Greif, Robert Carmona, Maria José Carvalho Böttiger, Bernd Walter Nakagawa, Naomi Kondo

Clinics

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2024, Volume 79 elocation 100518

Abstract: En | Text: En | PDF: En

Abstract Education in basic life support is widely proposed to increase survival in out-of-hospital sudden cardiac arrest. The authors aimed to assess knowledge, skills, and attitudes, including willingness to help, regarding myocardial infarction and sudden cardiac arrest among university students of all fields of knowledge. Methods: An Ethical Research Committee approved this cross-sectional study. An electronic survey “KIDS SAVE LIVES BRAZIL” was sent to 58,862 students of 82 disciplines in three universities, aged ≥ 18 years. The survey covered three categories: knowledge, skills, and attitude. Each category was graded between 0 and 10 points (the highest). Results: Among students, 4,803 undergraduates (8.2 %) answered the survey, and were divided into three groups of disciplines: medicine (219, ~21.7 years, 38 % male), other-healthcare (n = 1,058; ~22.9 years; 36 % male), and non-health-care (n = 3,526; ~22.9 years; 35 % male). All three groups showed significant differences between them (p < 0.001). The non-health-care compared with medicine and other healthcare groups showed, respectively, the lowest median scores (25 %-75%) in knowledge (4.0 [0.0-9.3], 4.0 [4.0-8.0], and 4.0 [4.0-4.7]), skills (2.4 [1.2-3.3], 6.4 [4.0-8.3], 4.0 [2.4-6.2]), and attitude (5.9 [5.9-6.8], 7.3 [5.9-7.3], and 7.3 [5.9-7.3]). Conclusion: University students who answered the e-survey have the willingness to help victims suffering from myocardial infarction or sustaining sudden cardiac arrest. However, non healthcare students markedly lack the knowledge and skills to perform cardiopulmonary resuscitation and automated external defibrillation. These findings reveal a stark difference in basic life support competencies between students in related healthcare fields and those in non-health fields, emphasizing the need for universal basic life support training.

https://doi.org/10.1016/j.clinsp.2024.100518

NURSING TECHNICIANS' PROXEMIC BEHAVIORS DURING TRANSFUSIONS IN HOSPITALIZED CLIENTS
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Gonçalves, Francisco Gleidson de Azevedo

; Araújo, Sílvia Teresa Carvalho de

; Azevedo, Albert Lengruber de

; Ferreira, Priscila Brigolini Porfírio

; Torres, Danelia Gómez

; Pérez Júnior, Eugenio Fuentes

; Bastos, Soraia do Socorro Furtado

; Silva, Suely Francisco da

Texto & Contexto - Enfermagem

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2024, Volume 33 elocation e20230207

Abstract: En Pt Es | Text: En Pt Es | PDF: En | PDF: Pt | PDF: Es

https://doi.org/10.1590/1980-265x-tce-2023-0207en

Physiological, anatomical, and histochemical changes in lentil (Lens culinaris Medik) seeds at different stages of maturation
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Cunha, Priscila Torres

; Trancoso, Ana Clara Reis

; Dias, Denise Cunha Fernandes dos Santos

; Picoli, Edgar Augusto de Toledo

; Silva Junior, Rubens Alves da

; Nascimento, Warley Marcos

Journal of Seed Science

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2024, Volume 46 elocation e202446039

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Conhecer as alterações que ocorrem nas sementes durante o processo de maturação é importante para definir o ponto ideal de colheita e obter de sementes de alta qualidade. Neste trabalho, dados de anatomia, histoquímica e qualidade fisiológica de sementes de lentilha foram obtidos em diferentes estádios de maturação. Sementes de lentilha, cv. Precoz, foram produzidas em campo experimental da Universidade Federal de Viçosa. Foram colhidas vagens em cinco estádios de maturação: super-verde, verde, verde-amarelo, amarelo e marrom. A caracterização anatômica e histoquímica das sementes foi realizada em microscopia de luz, sendo os cortes corados com Azul de toluidina, Xylidine Ponceau (XP), Lugol e Vermelho de Rutênio. A qualidade fisiológica das sementes foi avaliada por meio dos testes de germinação, IVG, emergência de plântulas, condutividade elétrica e envelhecimento acelerado. Durante a maturação das sementes de lentilha, a deposição de reservas de proteínas e amido ocorre quando as vagens estão no estádio verde, se estabilizando a partir do estádio amarelo. Fissuras no tegumento foram observadas nas sementes obtidas de vagens marrons. O potencial de germinação das sementes foi semelhante nos diferentes estádios de maturação. Sementes obtidas de vagens amarelas tiveram maior vigor em relação às de vagens verdes e marrons.

ABSTRACT: Understanding the changes that occur in seeds during the maturation process is essential to determine the optimal harvest time and to obtain high quality seeds. In this study, anatomical, histochemical and physiological quality data of lentil seeds at different stages of maturation were evaluated. Lentil seeds, cv. Precoz, were produced in an experimental field at the Universidade Federal de Viçosa. Pods were harvested at five stages of maturity: super-green, green, green-yellow, yellow and brown. The anatomical and histochemical characterization of the seeds was performed by light microscopy, with sections stained with Toluidine Blue, Xylidine Ponceau (XP), Lugol and Ruthenium Red. The physiological quality of the seeds was evaluated by germination, seedling emergence index (IVG), seedling emergence, electrical conductivity and accelerated ageing. During lentil seed maturation, protein, and starch reserve deposition occurs when the pods are at the green stage and stabilizes from the yellow stage. Seed coat cracks were observed in seeds from brown pods. Seed germination was similar among the different stages of maturity. Seeds from yellow pods were more vigorous than those from green and brown pods.

https://doi.org/10.1590/2317-1545v46291663

10.

Prevalence of clinical signs and diagnosis of temporomandibular disorders in adults with intellectual disability
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PUPO, Yasmine Mendes

; REIS, Giselle Emilãine da Silva

; DEZANETTI, Jullyana Mayara Preizner

; FANDERUFF, Marina

; SCHOSSLER, Thábata Louise

; PETTERLE, Ricardo Rasmussen

; CAVALHEIRO, Amanda Kerin Alves

; BONOTTO, Daniel

; TORRES-PEREIRA, Cassius Carvalho

; HILGENBERG-SYDNEY, Priscila Brenner

Revista de Odontologia da UNESP

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2024, Volume 53 elocation e20240031

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução A dor temporomandibular em indivíduos com deficiência intelectual foi explorada em apenas alguns estudos. No entanto, observa-se que a dor nessa população frequentemente permanece não detectada e inadequadamente tratada. Objetivo Avaliar e comparar os sinais e o diagnóstico de disfunção temporomandibular (DTM) em adultos com e sem deficiência intelectual (DI). Material e método Foi realizado um estudo transversal com grupo de comparação. Dados referentes ao gênero e à idade do grupo com DI foram coletados a partir de prontuários médicos disponíveis nas instituições de educação especial. Esses dados do grupo de comparação foram coletados nas clínicas odontológicas da Universidade Federal do Paraná (UFPR). Utilizamos o eixo I do RDC/TMD, com o auxílio da UPAT. O exame clínico avaliou dor muscular e articular, padrão de abertura bucal, extensão dos movimentos mandibulares e ruídos articulares. O teste do Qui-quadrado, o teste de Fisher e o pós-teste de Bonferroni foram utilizados com nível de significância de 5%. Resultado Foram avaliados dois grupos homogêneos em relação ao gênero (P = 0,08) e idade (P = 0,419), dos quais 97 adultos tinham deficiência intelectual e outros 96 não tinham deficiência intelectual. Não foi observada diferença significativa entre os grupos em relação aos sinais de DTM (p>0,05). O grupo de comparação apresentou significativamente mais deslocamento de disco com redução (DDCR) (P=0,011). Quando comparamos as diferenças em relação ao gênero, as mulheres do grupo de comparação tiveram maior prevalência de diagnósticos de DTM (p<0,05), mas essas diferenças não foram encontradas no grupo com DI (p>0,05). Em relação à idade, apenas o grupo de comparação mostrou significância estatística, com o diagnóstico de dor miofascial com abertura limitada sendo mais diagnosticado em indivíduos jovens (p=0,009). Conclusão Adultos com DI têm prevalência semelhante de sinais e sintomas de DTM em comparação com adultos sem DI. As diferenças de gênero não são significativas no grupo com DI.

Abstract Introduction Temporomandibular Pain among individuals with intellectual disabilities has been explored in only a few studies. Nevertheless, it is noted that pain in this population often remains undetected and inadequately treated. Objective To evaluate and compare, signs and diagnosis of temporomandibular disfunction (TMD) in adults with and without intellectual disability (ID). Material and method A cross-sectional study with comparison group was conducted. Data regarding gender and age of ID group were collected from medical records available in the institutions of special education. These data from the comparison group were collected in the dental clinics of the Federal University of Parana (UFPR). We using the RDC/TMD I axis, with the help of the UPAT. The clinical examination evaluated muscle and joint pain, mouth opening pattern, extension of mandibular movement and joint noise. The Chi-squared test, Fisher's test, and Bonferroni's post-test were used with significance level of 5%. Result Two homogeneous groups by gender (P = 0.08) and age (P = 0.419) were evaluated, of which 97 adults with intellectual disability and another 96 did not have intellectual disability. No significant difference was observed between groups on TMD signs (p>0.05). The comparison group had significantly more disc displacement with reduction (DDWR) (P=0.011). When we compare the differences with respect to gender. Overall, women in the comparison group had higher prevalence for TMD diagnoses (p<0.05), but these differences were not found in the ID group (p>0.05). Regarding age, only the comparison group showed statistical significance, with the diagnosis of myofascial pain with limited opening being more diagnosed in young individuals (p=0.009). Conclusion Adults with ID have similar prevalence of TMD signs and symptoms to adults without ID. Gender differences are not significant in the ID group.

https://doi.org/10.1590/1807-2577.03124

11.

Brazilian Research Consortium on Obsessive-Compulsive Spectrum Disorders guidelines for the treatment of adult obsessive-compulsive disorder. Part II: cognitive-behavioral therapy
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Mathis, Maria Alice de

; Chacon, Priscila

; Boavista, Rodrigo

; de Oliveira, Marcos Vinícius Sousa

; de Barros, Pedro Macul Ferreira

; Echevarria, Marco Antonio Nocito

; Ferrão, Ygor Arzeno

; Vattimo, Edoardo Filippo de Queiroz

; Lopes, Antônio Carlos

; Torres, Albina Rodrigues Diniz, Juliana Belo Fontenelle, Leonardo do Rosário, Maria Conceição Shavitt, Roseli Gedanke da Silva, Renata de Melo Felipe Miguel, Eurípedes Constantino Costa, Daniel Lucas da Conceição

Brazilian Journal of Psychiatry

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oct 2023, Volume 45 N. 5 Pages 431 - 447

Abstract: En | Text: En | PDF: En

Objectives: To summarize evidence-based cognitive-behavioral therapy (CBT) treatment and propose clinical interventions for adult patients with obsessive-compulsive disorder (OCD). Methods: The literature on CBT interventions for adult OCD, including BT and exposure and response prevention, was systematically reviewed to develop updated clinical guidelines for clinicians, providing comprehensive details about the necessary procedures for the CBT protocol. We searched the literature from 2013-2020 in five databases (PubMed, Cochrane, Embase, PsycINFO, and Lilacs) regarding study design, primary outcome measures, publication type, and language. Selected articles were assessed for quality with validated tools. Treatment recommendations were classified according to levels of evidence developed by the American College of Cardiology and the American Heart Association. Results: We examined 44 new studies used to update the 2013 American Psychiatric Association guidelines. High-quality evidence supports CBT with exposure and response prevention techniques as a first-line treatment for OCD. Protocols for Internet-delivered CBT have also proven efficacious for adults with OCD. Conclusion: High-quality scientific evidence supports the use of CBT with exposure and response prevention to treat adults with OCD.

https://doi.org/10.47626/1516-4446-2023-3081

12.

Brazilian Research Consortium on Obsessive-Compulsive Spectrum Disorders guidelines for the treatment of adult obsessive-compulsive disorder. Part I: pharmacological treatment ObsessiveCompulsive Obsessive Compulsive obsessivecompulsive obsessive compulsive disorder I
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de Oliveira, Marcos Vinícius Sousa

; de Barros, Pedro Macul Ferreira

; de Mathis, Maria Alice

; Boavista, Rodrigo

; Chacon, Priscila

; Echevarria, Marco Antonio Nocito Ferrão, Ygor Arzeno Vattimo, Edoardo Felippo de Queiroz Lopes, Antônio Carlos Torres, Albina Rodrigues Diniz, Juliana Belo Fontenelle, Leonardo F. Rosário, Maria Conceição do Shavitt, Roseli Gedanke Miguel, Eurípedes Constantino da Silva, Renata de Melo Felipe Costa, Daniel Lucas da Conceição

Brazilian Journal of Psychiatry

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apr 2023, Volume 45 N. 2 Pages 146 - 161

Abstract: En | Text: En | PDF: En

Objectives: To summarize evidence-based pharmacological treatments and provide guidance on clinical interventions for adult patients with obsessive-compulsive disorder (OCD). Methods: The American Psychiatric Association (APA) guidelines for the treatment of OCD (2013) were updated with a systematic review assessing the efficacy of pharmacological treatments for adult OCD, comprising monotherapy with selective serotonin reuptake inhibitors (SSRIs), clomipramine, serotonin and norepinephrine reuptake inhibitors (SNRIs), and augmentation strategies with clomipramine, antipsychotics, and glutamate-modulating agents. We searched for the literature published from 2013-2020 in five databases, considering the design of the study, primary outcome measures, types of publication, and language. Selected articles had their quality assessed with validated tools. Treatment recommendations were classified according to levels of evidence developed by the American College of Cardiology and the American Heart Association (ACC/AHA). Results: We examined 57 new studies to update the 2013 APA guidelines. High-quality evidence supports SSRIs for first-line pharmacological treatment of OCD. Moreover, augmentation of SSRIs with antipsychotics (risperidone, aripiprazole) is the most evidence-based pharmacological intervention for SSRI-resistant OCD. Conclusion: SSRIs, in the highest recommended or tolerable doses for 8-12 weeks, remain the first-line treatment for adult OCD. Optimal augmentation strategies for SSRI-resistant OCD include low doses of risperidone or aripiprazole. Pharmacological treatments considered ineffective or potentially harmful, such as monotherapy with antipsychotics or augmentation with ketamine, lamotrigine, or N-acetylcysteine, have also been detailed. Objectives evidencebased based obsessivecompulsive obsessive compulsive . (OCD) Methods (APA (2013 , (SSRIs) clomipramine SNRIs, SNRIs (SNRIs) glutamatemodulating glutamate modulating agents 20132020 2020 2013-202 databases study measures publication language tools ACC/AHA. ACCAHA ACC/AHA ACC AHA (ACC/AHA) Results 5 201 Highquality High firstline first line Moreover risperidone, (risperidone aripiprazole SSRIresistant SSRI resistant Conclusion 812 8 12 8-1 weeks harmful ketamine lamotrigine Nacetylcysteine, Nacetylcysteine N acetylcysteine, acetylcysteine N-acetylcysteine detailed (OCD (201 (SSRIs (SNRIs 2013202 202 2013-20 (ACC/AHA 20 81 1 8- (20 201320 2013-2 2 (2 20132 2013- (

https://doi.org/10.47626/1516-4446-2022-2891

13.

IL17A and IL17RA gene polymorphisms in Fanconi anemia ILA IL A ILRA RA
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MOBILE, Rafael Zancan

; MENDES, Monalisa Castilho

; MACHADO-SOUZA, Cleber

; QUEIROZ, Priscila de Mattos

; BONFIM, Carmem Maria Sales

; TORRES-PEREIRA, Cassius Carvalho

; SCHUSSEL, Juliana Lucena

Brazilian Oral Research

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2023, Volume 37 elocation e012

Abstract: En | Text: En | PDF: En

Abstract Fanconi anemia is a rare autosomal recessive disease. In this disease, cytokine pathways can induce the bone marrow failure that is observed in individuals with Fanconi anemia. Interleukin IL-17 exhibits a protective effect in organisms because it induces neutrophil recruitment and shows a pathological role in several models of autoimmune diseases, periodontal disease, cancer, allograft rejection, and graft versus host disease. Polymorphisms in the IL17A and IL17RA genes were evaluated from DNA in saliva, comparing individuals with or without Fanconi anemia, using models of genotypic transmission (additive, dominant, and recessive). Polymorphisms in the IL17A and IL17RA genes (rs2241044 [C allele], rs879577 [C allele], rs9606615 [T allele], and rs2241043 [C allele]) were risk factors for developing Fanconi anemia. We also performed an analysis of gene markers with clinical variables in the Fanconi group. Polymorphisms in the IL17A gene (rs3819025 [A allele] and rs2275913 [G allele], respectively) were associated with an age of less than 20 years (p = 0.026; RP 0.65) and the female sex (p = 0.043; RP 0.88). The IL17RA gene was also associated with age and the presence of leukoplakia (a potentially malignant oral disorder). An age of less than 20 years was associated with rs917864 (T allele; p = 0.036; RP 0.67). The presence of leukoplakia was associated with rs17606615 (T allele; p = 0.042; RP 0.47). To our knowledge, this is the first study that associates IL17A and IL17RA gene polymorphisms with Fanconi anemia and examines rs2241044 polymorphisms in scientific literature thus far. disease IL17 IL 17 IL-1 diseases cancer rejection ILA A ILRA RA saliva additive, additive (additive dominant recessive. . recessive) rs (rs224104 C allele, allele , rs87957 rs960661 T rs224104 group rs3819025 (rs381902 rs227591 G respectively 2 0.026 0026 0 026 0.65 065 65 0.043 0043 043 0.88. 088 0.88 88 0.88) disorder. disorder disorder) rs91786 0.036 0036 036 0.67. 067 0.67 67 0.67) rs1760661 0.042 0042 042 0.47. 047 0.47 47 0.47) knowledge far IL1 1 IL- (rs22410 rs8795 rs96066 rs22410 rs381902 (rs38190 rs22759 0.02 002 02 0.6 06 6 0.04 004 04 08 0.8 8 rs9178 0.03 003 03 rs176066 0.4 4 (rs2241 rs879 rs9606 rs2241 rs38190 (rs3819 rs2275 0.0 00 0. rs917 rs17606 (rs224 rs87 rs960 rs224 rs3819 (rs381 rs227 rs91 rs1760 (rs22 rs8 rs96 rs22 rs381 (rs38 rs9 rs176 (rs2 rs2 rs38 (rs3 rs17 (rs rs3 rs1

https://doi.org/10.1590/1807-3107bor-2023.vol37.0012

14.

An outbreak of fatal Pullorum disease (Salmonella Pullorum) in Guinea fowl keets (Numida meleagris) Salmonella Numida meleagris
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Pinto, Priscila N.

; Torres, Ana C.D.

; Rodrigues, Mariana P.

; Oliveira, Letícia B.

; Costa, Camila S.

; Ecco, Roselene

; Freitas Neto, Oliveiro C.

; Martins, Nelson R.S.

Pesquisa Veterinária Brasileira

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2023, Volume 43 elocation e07088

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A pulorose é descrita mundialmente e é causada por Salmonella enterica subespécie enterica sorovar Gallinarum biovar Pullorum (S. Pullorum). A infecção por S. Pullorum é importante em aves comerciais, provocando doença sistêmica com altas taxas de mortalidade. Sua ocorrência requer notificação e quando diagnosticada em aves de criação comercial resulta na erradicação do plantel. O objetivo deste estudo foi relatar um surto grave de pulorose em filhotes de galinhas-d’Angola (Numida meleagris), resultando em 100% de mortalidade das aves (n=290) nas primeiras duas semanas de idade. Os pintinhos recebidos tinham hepato, espleno e nefromegalia (5/5). Os tecidos dos cinco indivíduos recebidos foram submetidos a exame histológico e bacteriológico. Na histopatologia, foram observados nódulos paratifoides aleatórios caracterizados por áreas de necrose com fibrina e infiltrado moderado de macrófagos e heterófilos no fígado. Nos rins, foram observadas áreas discretas de necrose associadas a infiltrados multifocais moderados de linfócitos e plasmócitos. No baço, foi observado infiltrado moderado de macrófagos. O isolamento de colônias sugestivas de S. Pullorum de fígados e baços foi realizado em ágares seletivos e, após testes bioquímicos, confirmado por duplex-PCR específico. A susceptibilidade antimicrobiana da cepa isolada revelou resistência apenas ao sulfametoxazol + trimetoprim entre os antimicrobianos testados. O isolado de S. Pullorum recuperado no presente estudo foi altamente patogênico para N. meleagris e pode representar um risco para outras espécies de aves, incluindo aves industriais. RESUMO S (S Pullorum. . Pullorum) comerciais plantel galinhasdAngola galinhas d Angola Numida meleagris, , meleagris) 100 n=290 n290 n 290 (n=290 idade hepato 5/5. 55 5/5 5 (5/5) bacteriológico histopatologia fígado rins plasmócitos baço bioquímicos duplexPCR duplex PCR específico testados N industriais 10 n=29 n29 29 (n=29 5/ (5/5 1 n=2 n2 2 (n=2 (5/ n= (n= (5 (n (

ABSTRACT: Pullorum disease is described worldwide and is caused by Salmonella enterica subspecies enterica serovar Gallinarum biovar Pullorum (S. Pullorum). S. Pullorum infection is important in commercial poultry, provoking a systemic disease with high mortality rates. Its occurrence requires notification, and when it is diagnosed in commercial breeding flocks, its eradication is demanded. The aim of this study was to report a severe outbreak of Pullorum disease in young Guinea fowl (Numida meleagris), resulting in 100% mortality of keets (n=290) within the first two weeks of age. All examined keets had enlarged liver, kidneys and spleen (5/5), and the affected tissues were submitted to histological and bacteriological examination. On histopathology, random paratyphoid nodules characterized by areas of necrosis with fibrin and a moderate infiltrate of macrophages and heterophils were observed in the liver. In kidneys, discrete areas of necrosis associated with moderate multifocal infiltrates of lymphocytes, and plasma cells were observed. In the spleen, a moderate infiltrate of macrophages was noticed. Isolation of colonies suggestive of S. Pullorum from liver and spleen was performed in selective agars and, after biochemical tests, confirmed by specific duplex-PCR. The antimicrobial susceptibility test of the isolated strain revealed resistance to only sulfamethoxazole + trimethoprim among the tested antimicrobials. The S. Pullorum isolate recovered in the present study was highly pathogenic to N. meleagris and may represent a risk to other avian species, including industrial poultry. ABSTRACT S (S Pullorum. . Pullorum) poultry rates notification flocks demanded Numida meleagris, , meleagris) 100 n=290 n290 n 290 (n=290 age 5/5, 55 5/5 5 (5/5) examination histopathology lymphocytes noticed tests duplexPCR. duplexPCR duplex PCR. PCR duplex-PCR antimicrobials N species 10 n=29 n29 29 (n=29 5/ (5/5 1 n=2 n2 2 (n=2 (5/ n= (n= (5 (n (

https://doi.org/10.1590/1678-5150-pvb-7088

15.

Construção e Validação do Protocolo EmpoderACO Direcionado a Pacientes em Anticoagulação Oral com Varfarina
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Barbosa, Hannah Cardoso

; Torres, Heloisa de Carvalho

; Oliveira, João Antônio de Queiroz Santos, Rebeca Priscila de Melo Costa, Josiane Moreira da Miranda, Leonardo Gonçalves Pagano, Adriana Silvina Praxedes, Marcus Fernando da Silva Martins, Maria Auxiliadora Parreiras

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 N. 6 elocation e20220576

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Background Warfarin is an oral anticoagulant that is very useful in preventing thromboembolism, though it is considered a drug with a high risk of causing adverse events. Considering the practical challenges in controlling oral anticoagulation, the patients on warfarin could benefit from educational strategies aimed at behavioral changes, active participation in self-care, and adherence to drug therapy. Objective The aim was to construct and validate the EmpoderACO protocol for behavioral changes in warfarin patients. Methods The methodological steps were: definition of concepts and domains of self-care, identification of objectives, construction and selection of items, assessment of content validity, and pre-test in the target population. Results Relevance, adequacy, clarity, and internal reliability of the instrument’s items were assessed by a multidisciplinary judges committee (JC) through the E-surv web platform, obtaining an average agreement of ≥0.91. The understanding of the instrument measured by the target population revealed adequate clarity with a coefficient average of 0.96. Conclusion EmpoderACO can aid in qualifying the communication process between medical professionals and patients, as well as in improving adherence to both treatment and clinical outcomes, and can be replicated in healthcare settings.

Resumo Fundamento A varfarina é um anticoagulante oral útil para prevenção de tromboembolismo, embora seja considerado fármaco de alto risco de causar eventos adversos. Considerando os desafios práticos no controle da anticoagulação oral, os pacientes poderiam se beneficiar de estratégias educacionais que visem mudança de comportamento, participação ativa no autocuidado e adesão à farmacoterapia. Objetivo Construir e validar o protocolo EmpoderACO para mudança de comportamento em pacientes em uso de varfarina. Métodos As etapas metodológicas foram: definição de conceitos e domínios do autocuidado, identificação dos objetivos, construção e seleção dos itens, avaliação da validade de conteúdo e pré-teste na população alvo. Resultados Relevância, adequação, clareza e confiabilidade interna dos itens do instrumento foram avaliadas por comitê de juízes multiprofissional pela plataforma web E-surv, obtendo-se média de concordância ≥0,91. A compreensão do instrumento pela população-alvo teve clareza adequada com média de 0,96. Conclusão O EmpoderACO poderá contribuir para qualificar o processo de comunicação entre profissionais e pacientes, melhorar a adesão ao tratamento e os resultados clínicos, podendo ser replicado nos serviços de saúde.

https://doi.org/10.36660/abc.20220576

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