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Reduced genetic diversity and the success of the invasive peacock bass (Cichliformes: Cichlidae)
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Luz, S. C. S.

; Montes, M. A.

; Farias, R. S.

; Lima, R. V.

; Torres, R. A.

; Ferreira, E. J. G.

; Severi, W.

; Coimbra, M. R. M.

Brazilian Journal of Biology

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2024, Volume 84 elocation e248656

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Muitas espécies de Cichla colonizaram com sucesso lagos e reservatórios do Brasil desde os anos 1960, causando graves prejuízos à vida selvagem nesses locais. Neste estudo, 135 tucunarés foram coletados em um complexo de reservatórios a fim de identificar se representavam uma espécie dominante ou múltiplas espécies, uma vez que diversas espécies de Cichla foram registradas na bacia. Os espécimes foram identificados com base na coloração, dados morfométricos e merísticos, e por marcadores mitocondriais COI, 16S rDNA e Região Controle (RC). A sobreposição dos dados morfométricos e o padrão similar de coloração impediram a identificação utilizando as chaves de identificação disponíveis na literatura. Entretanto, as análises bayesiana e de máxima verossimilhança de dados moleculares demonstraram a ocorrência de uma única espécie, Cichla kelberi. Um único haplótipo foi observado para o 16S e RC, enquanto três foram detectados para o COI, com um haplótipo dominante presente em 98,5% das amostras. A baixa diversidade nos exemplares introduzidos de C. kelberi evidenciou um número limitado de linhagens maternas fundadoras. O sucesso da invasão parece depender de fatores abióticos, como a maior transparência da água de ambientes lênticos que favorece predadores visuais que, atrelado à ausência de predadores, fez do C. kelberi um invasor bem-sucedido nesses reservatórios.

Abstract Several species of Cichla successfully colonized lakes and reservoirs of Brazil, since the 1960’s, causing serious damage to local wildlife. In this study, 135 peacock bass were collected in a reservoir complex in order to identify if they represented a single dominant species or multiple ones, as several Cichla species have been reported in the basin. Specimens were identified by color pattern, morphometric and meristic data, and using mitochondrial markers COI, 16S rDNA and Control Region (CR). Overlapping morphological data and similar coloration patterns prevented their identification using the taxonomic keys to species identification available in the literature. However, Bayesian and maximum likelihood from sequencing data demonstrated the occurrence of a single species, Cichla kelberi. A single haplotype was observed for the 16S and CR, while three were detected for COI, with a dominant haplotype present in 98.5% of the samples. The extreme low diversity of the transplanted C. kelberi evidenced a limited number of founding maternal lineages. The success of this colonization seems to rely mainly on abiotic factors, such as increased water transparency of lentic environments that favor visual predators that along with the absence of predators, have made C. kelberi a successful invader of these reservoirs.

https://doi.org/10.1590/1519-6984.248656 101 downloads

Inactivation of isolated fungi on Erythrina velutina Willd. seeds through atmospheric plasma
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Diógenes, F. E. P.

; Nascimento, S. R. C.

; Alves Junior, C.

; Paiva, E. P.

; Torres, S. B.

; Oliveira, A. K.

; Ambrósio, M. M. Q.

Brazilian Journal of Biology

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2024, Volume 84 elocation e251367

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Este trabalho teve como objetivo avaliar o efeito da aplicação de plasma atmosférico na inativação de fungos na superfície de sementes de Erythrina velutina e em colônias fúngicas isoladas. Dois experimentos foram realizados em delineamento inteiramente casualizado: no primeiro, o plasma foi aplicado na superfície das sementes usando gás hélio e plasma atmosférico por três, seis e nove minutos, além do controle (sementes sem tratamento), constituindo sete tratamentos com cinco repetições cada; no segundo experimento, placas de Petri contendo o inóculo de diferentes fungos foram tratadas com plasma atmosférico por três, seis e nove minutos (Air-3, Air-6 e Air-9) e comparadas com fungos não tratados em placas de Petri sem tratamento (controle), totalizando quatro tratamentos e cinco repetições cada. Descobrimos que a aplicação de plasma atmosférico nas sementes de E. velutina por nove minutos teve efeito antimicrobiano sobre os fungos Aspergillus niger, Aspergillus flavus, Fusarium sp., Brachysporium sp. e Rhizopus sp. A formação de colônias fúngicas isoladas de sementes de E. velutina também foi inibida por três minutos de exposição à aplicação de plasma atmosférico, exceto para A. niger, cuja inibição ocorreu a partir de 6 minutos de exposição à aplicação de plasma atmosférico.

Abstract This study aimed to evaluate the effect of atmospheric plasma application on the inactivation of fungi on the surface of Erythrina velutina seeds and on isolated fungal colonies. Two experiments were conducted using a completely randomized design. First, plasma was applied to the surface of the seeds using helium gas and atmospheric plasma for 3, 6, and 9 min in addition to the control (untreated seeds), constituting seven treatments with five repetitions each. In the second experiment, Petri dishes containing the inoculum of different fungi were treated with atmospheric air plasma for 3, 6, and 9 min (Air-3, Air-6, and Air-9) and were compared with untreated fungi in Petri dishes without treatment (control), totaling four treatments and five repetitions each. We found that the application of atmospheric air plasma to E. velutina seeds for 9 min had an antimicrobial effect on the fungi Aspergillus niger, Aspergillus flavus, Fusarium sp., Brachysporium sp., and Rhizopus sp. The formation of fungal colonies isolated from E. velutina seeds was also inhibited by 3 min of exposure to atmospheric air plasma, except for A. niger, whose inhibition occurred after 6 min of exposure to atmospheric plasma.

https://doi.org/10.1590/1519-6984.251367 1 downloads

Evaluation of an automatized AMBU ESSI-1 INC designed at the Instituto Nacional de Cardiología Ignacio Chávez
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Abundes-Velasco, Arturo

; Sánchez-Pérez, Efraín

; Jiménez-Rodríguez, Gian M.

; Molina-Méndez, Francisco J.

; Herrera-Elizalde, Rafael

; Pulido-Zamudio, Tomás

; Álvarez-Rosales, Humberto

; Villegas-Juache, Juan

; Aceves-Díaz-González, Sebastián

; Parra-Rodríguez, David A.

; Cruz-Enríquez, Francisco J.

; Cobos-Reyes, Farid A.

; Aguilar-Torres, Eduardo A.

; Islas-Navarro, Enid A.

; Nuñez-Gómez, José C.

; Peña-Duque, Marco A.

Archivos de cardiología de México

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dec 2023, Volume 93 N. 4 Pages 391 - 397

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Objetivo: Evaluar un dispositivo automatizado para la asistencia ventilatoria basado en un AMBU manufacturado en Marzo del 2020. Métodos: El ESSI-1 INC fue evaluado por medio de parámetros fisiológicos y mecánica pulmonar a través de pruebas de espirómetro compensatorios (TISSOT); pulmón artificial (Modelo 5600i Dual Adult PNEU VIEW SYSTEM); así como su desempeño comparado a la máquina de anestesia (AEONMED 7500) en modelos porcinos, midiendo criterios ventilatorios, hemodinámicos y gasométricos. Resultados: Este equipo (ESSI-1 INC) fue exitosamente probado por modelos mecánicos y biológicos, tales como cerdos donde su desempeño fue evaluado en términos de la variabilidad del volumen tidal, frecuencia ventilatoria, y relación I/E versus el desempeño manual de dos médicos. Todos los resultados finalizaron como se esperaba de forma satisfactoria. Conclusiones: Es un equipo seguro y efectivo, el cual debería ser probado y usado en distintas condiciones clínicas para estandarizar la seguridad ventilatoria y cuidado de pacientes que lo requieran.

Abstract Objective: The objectives of this study were to evaluate an automated device for ventilatory support based on AMBU manufactured in March 2020. Methods: The ESSI-1 INC was evaluated through pulmonary mechanics and physiology parameters through compensatory spirometer tests (TISSOTs), and an artificial lung Model5600i Dual Adult PNEU VIEW SYSTEM; it was also compared to the anesthetic ventilatory support equipment (AEONMED 7500) in porcine models, measuring ventilatory, hemodynamic and gasometric parameters. Results: This equipment (ESSI-1 INC) was successfully tested by mechanical and biological models, such as pigs in which its performance was evaluated in terms of variability of tidal volume, ventilation frequency, and I/E relationship versus the manual performance of two medical interns. All the results turned out as expected and were satisfactory. Conclusions: It is safe and effective equipment and should be tested and used in diverse clinical conditions to standardize the ventilatory safety and care of patients who require it.

https://doi.org/10.24875/acm.2200004

Effect of school reopening on pediatric morbidity and mortality during the third epidemiological wave of COVID-19 in a Mexican state
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Reyna-Figueroa, Jesús

; Mirón-Calderón, Xochitl

; Durán-Mendieta, Victor

; Ramirez-Gijón, Guillermo

; Torres-Meza, Victor

; Anaya-López, Luis

; Frías-Badillo, Juan C.

; Mejía-Martínez, Valeria

; Salyano-Peñuelas, Yolanda A.

; Diaz-Martínez, Alfredo I.

; Fernández-Clamont, Francisco J.

Boletín médico del Hospital Infantil de México

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dec 2023, Volume 80 N. 6 Pages 367 - 373

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción: La determinación del efecto de reabrir las escuelas sobre las tasas de infección pediátrica por SARS-CoV-2 (síndrome respiratorio agudo grave coronavirus 2) incrementó la necesidad de trasmitir la experiencia de los gobiernos de muchas regiones geográficas para mejores decisiones futuras en emergencias sanitarias similares. Métodos: Mediante un estudio prospectivo basado en una cohorte poblacional se dio seguimiento a los alumnos de 18,988 escuelas del Estado de México que iniciaron con el regreso a clases. Se implementaron filtros sanitarios diarios en cada escuela y cotidianamente se informaban a los enlaces jurisdiccionales a través de una red negativa. Los casos identificados eran confirmados a través de RT-PCR (reacción en cadena de la polimerasa con transcriptasa inversa). Se estimaron frecuencias simples de casos, porcentajes e incidencias de COVID-19. Las incidencias del estado se compararon con la incidencia nacional. Resultados: Un total de 3,586 casos fueron confirmados; 2,048 (57.1%) correspondieron a niños. Veinticuatro (0.6%) fueron hospitalizados por COVID-19 moderado a grave; nueve (37.5%) fallecieron, y solamente una correspondió a un escolar. A partir de la semana 36 se observó una tasa promedio de infecciones de 0.36. En la semana epidemiológica 40 se observó la mayor tasa de infección en escolares (1.01); a partir de esta semana se observa un declive de los casos hasta la semana 50. Conclusiones: La implementación de intervenciones no farmacéuticas tiene más ventajas que limitaciones, siempre y cuando las estrategias sean homogéneas y correctamente ejecutadas, lo que asegurará un adecuado control en los contagios.

Abstract Background: Determining the effect of reopening schools on pediatric SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) infection rates increased the need to share the experience of governments in many geographic regions for better future decision-making in similar health emergencies. Methods: Through a prospective study based on a population-based cohort, students from 18,988 schools in the State of Mexico who began returning to school were followed. Daily sanitation filters were implemented in each school and district liaisons were informed on a daily basis through a negative network. Identified cases were confirmed by reverse transcriptase-polymerase chain reaction. Simple case frequencies, percentages, and incidences of COVID-19 were estimated. State incidences were compared with the national incidence. Results: A total of 3,586 cases were confirmed; 2,048 (57.1%) were children. Twenty-four (0.6%) were hospitalized for moderate to severe COVID-19; nine (37.5%) died, and only one was a schoolchild. From week 36, an average infection rate of 0.36 was observed. The highest infection rate in schoolchildren was observed in epidemiologic week 40 (1.01); from this week on, a decrease in the number of cases was observed until week 50. Conclusions: The use of non-pharmaceutical interventions has more advantages than limitations, as long as the strategies are homogeneous and properly implemented to ensure adequate control of infections.

https://doi.org/10.24875/bmhim.23000075

Estimate of carbon stock in the soil via diffuse reflectance spectroscopy (vis/nir) air and orbital remote sensing
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Faria, Ohana C. O.

; Torres, Gilmar N.

; Raimo, Luis A. D. L. Di

; Couto, Eduardo G.

Revista Caatinga

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sep 2023, Volume 36 N. 3 Pages 675 - 689

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Current procedures for determining soil organic carbon (SOC) content are costly, time-consuming, and generate polluting chemical waste. Therefore, developing new protocols using aerial and orbital remote sensing and diffuse reflectance spectroscopy (DRS) for digitally mapping the stock of soil organic carbon (CS) is essential for promoting actions of research and monitoring SOC in Brazilian soils. Given this, three areas of commercial plots in the region of the Middle North of Mato Grosso were studied, where sampling was carried out for the determination of SOC in the layer from 0 to 30 cm, evaluated by the dry combustion method and estimated through DRS in the visible to near -infrared region - Vis-NIR-SWIR/350-2500 nm). To obtain the images by aerial remote sensing, the Carcará II® Unmanned Aerial Vehicle was used, with a MicaSense® multispectral camera (RGB + NIR + RedEdge) attached. The orbital sensors used were the Sentinel 2® and Planet® satellites. This study showed that soil carbon stock values could be predicted using different modeling approaches based on field and laboratory spectral measurements. Predictive models to estimate SOC can be established using remote and near sensing, thus allowing a better understanding of spatial patterns of SOC in crop fields.

RESUMO Os procedimentos atuais de determinação do conteúdo de carbono orgânico do solo (COS) são onerosos, demorados e geram resíduos químicos poluentes. Por isso, o desenvolvimento de novos protocolos utilizando o sensoriamento remoto aéreo, orbital e a espectroscopia de refletância difusa (ERD) para o mapeamento digital do estoque de carbono orgânico do solo (EC) são imprescindíveis para o fomento de ações de pesquisa e monitoramento do COS nos solos brasileiros. Diante disso, foram estudadas três áreas de talhões comerciais na região do Meio Norte de Mato Grosso onde realizou-se a amostragem para a determinação de COS na camada de 0 a 30 cm, avaliado pelo método de combustão via seca e estimado através da ERD na região do visível ao infravermelho-próximo - Vis-NIR-SWIR/350-2500 nm) . Para obtenção das imagens por sensoriamento remoto aéreo, foi utilizado o Veículo Aéreo Não Tripulado Carcará II®, com uma câmera multiespectal (RGB + NIR + RedEdge) da marca MicaSense® acoplada. Os sensores orbitais utilizados foram o satélite Sentinel 2® e Planet®. Este estudo mostrou que os valores do estoque de carbono do solo podem ser preditos usando diferentes abordagens de modelagem com base em medições espectrais de campo e laboratório. Modelos preditivos para estimar o COS podem ser estabelecidos usando sensoriamento remoto e próximo, permitindo assim uma melhor compreensão dos padrões espaciais do COS nos campos de cultivo.

https://doi.org/10.1590/1983-21252023v36n320rc

Esfuerzos en la detección de aneurismas aórticos y la formación de recursos humanos para la optimización de su tratamiento
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Hinojosa-Becerril, Carlos A.

; Anaya-Ayala, Javier E.

; Bobadilla-Rosado, Luis

; Arámburo-López, Juan C.

; Barragán-Galindo, Luis

; Lozano-Corona, Rodrigo

; Torres-Machorro, Adriana

; Laparra-Escareno, Hugo

Cirugía y cirujanos

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aug 2023, Volume 91 N. 4 Pages 514 - 520

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Objetivo: Revisar los ingresos, procedimientos y defunciones intrahospitalarias asociadas a aneurismas aórticos abdominales (AAA) y analizar el impacto de la introducción de programas de formación de recursos humanos y tamizaje ultrasonográfico. Métodos: Estudio retrospectivo, se analizaron las bases de datos nacionales obtenidas del portal datos abiertos de la Dirección General de Información en Salud (DGIS) del año 2010 al 2020. Se calculó la letalidad intrahospitalaria anual y comparamos la experiencia del Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ). El análisis estadístico se realizó en el programa STATA versión 17. Resultados: De acuerdo con la base nacional (BN), se registraron 899 (91%) ingresos, mientras que en el INCMNSZ 85 (9%). La mayoría pertenecía al sexo masculino (68%), un total de 811 (82%) pacientes fueron sometidos a cirugía abierta, mientras que 173 (18%) a terapia endovascular (EVAR), siendo este abordaje más frecuente en nuestra institución (p = 0.007). La mortalidad intrahospitalaria fue del 22.5%, en la BN fue del 23.9%, mientras que en el INCMNSZ fue del 16.4%, sin que encontráramos diferencia significativa (p = 0.1). Conclusiones: Los AAA continúan siendo poco reconocidos en nuestro país. La introducción de programas universitarios de especialidad y el tamizaje podría impactar en la reducción de la morbimortalidad.

Abstract Objective: To review admissions, interventions and in-hospital mortality associated to Abdominal Aortic Aneurysms (AAA), and to analyze the impact of the introduction of a training program and imaging screening at our institution. Methods: Retrospective study where hospitalizations, procedures and mortality secondary to AAA were recorded. The national databases (ND) from the Secretariat of Health were utilized from 2010 to 2020. In-hospital lethality was calculated and compared with the experience at the Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ). The statistical analysis was completed with the STATA version 17. Results: According to the ND, 899 (91%) hospital admissions secondary to AAA occurred, while in the INCMNSZ 85 (9%). Most of them belonged to the male gender (68%); 811 (82%) patients underwent open surgical repair, and 173 (18%) to an endovascular exclusion (EVAR), the latter approach was significantly more frequently performed at our institution (p = 0.007). The 30-day hospital mortality was 22.5%; in the ND was 23.9 vs. a 16.4% in the INCMNSZ without significant difference (p = 0.1). Conclusions: AAA remain unrecognized in our country. The introduction of University programs and imaging screening might impact in the early detection, and to reduce the morbidity and mortality associated to emergency procedures.

https://doi.org/10.24875/ciru.22000300

La intención emprendedora en estudiantes de maestría en administración de empresas de universidades colombianas: el rol moderador del control del comportamiento percibido
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Acosta-Prado, Julio C.

; Zárate-Torres, Rodrigo A.

; Aburto-Camacllanqui, Elías

Formación universitaria

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jun 2023, Volume 16 N. 3 Pages 31 - 40

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: The main objective of this research study is to determine whether perceived behavioral control has a moderating role in the influence of attitude toward entrepreneurship and subjective norms on entrepreneurial intention. The sample consists of 159 master of business administration (MBA) students from four Colombian universities. The methodological design is cross-sectional and explanatory and applies the partial least squares structural equation modeling (PLS-SEM). The results show that there is no evidence to suggest that perceived behavioral control moderates the relationship between attitude toward entrepreneurship and entrepreneurial intention. In addition, there is no evidence to infer that perceived behavioral control moderates the relationship between subjective norms and entrepreneurial intention. In conclusion, the current model of the theory of planned behavior provides no empirical support for explaining the entrepreneurial intention of MBA students from four different Colombian universities.

Resumen: El objetivo de esta investigación es determinar si el control del comportamiento percibido tiene un rol moderador en la influencia de la actitud hacia el emprendimiento y las normas subjetivas sobre la intención emprendedora. La muestra consiste en 159 estudiantes de maestría en administración de empresas (MBA) de cuatro universidades privadas colombianas en Bogotá. El diseño metodológico es explicativo-transversal y utiliza el modelo de ecuaciones estructurales con mínimos cuadrados parciales (PLS-SEM). Los resultados muestran que no hay evidencia para afirmar que el control del comportamiento percibido modera la relación entre la actitud hacia el emprendimiento y la intención emprendedora. Tampoco hay evidencia de que el control del comportamiento percibido modera la relación entre las normas subjetivas y la intención emprendedora. En conclusión, el modelo actual de la teoría de la conducta planeada no tiene respaldo empírico en la explicación de la intención emprendedora de estudiantes de cuatro programas de MBA de universidades colombianas.

https://doi.org/10.4067/s0718-50062023000300031

Guía de práctica clínica para el manejo del cáncer de pulmón de células pequeñas: enfermedad extensa
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Cabrera-Miranda, Luis A.

; Díaz-García, Diego A.

; Corona-Cruz, José F.

; Lozano-Ruiz, Francisco J.

; Sánchez-Reyes, Roberto

; Álvarez-Bojórquez, Mario E.

; Blake-Cerda, Mónika

; Rivera-Márquez, Raúl

; López-Saucedo, Raúl A.

; Pérez-Álvarez, Sandra I.

; Bolaño-Guerra, Laura M.

; Alatorre-Alexander, Jorge A.

; Alexander-Meza, Francisco

; Barrón-Barrón, Feliciano

; Blanco-Vázquez, Yazmín C.

; Campos-Gómez, Saúl

; Mata-Moya, Dolores de la

; Figueroa-Martínez, Pedro

; González-Cisneros, Paulina E.

; Íñiguez-García, Marco A.

; Lázaro-León, Jesús M.

; Loyola-García, Ulises

; Morales-Rivera, Marcelino

; Olivares-Torres, Carlos

; Ramos-Ramírez, Maritza

; Sáenz-Frías, Julia A.

; Silva-Bravo, Fernando

; Trejo-Rosales, Rogelio

; Souto-del Bosque, Miguel

; Arrieta, Óscar

Gaceta mexicana de oncología

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jun 2023, Volume 22 N. 2 Pages 55 - 73

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Antecedentes: El cáncer de células pequeñas (CPCP) representa el 13-15% del total de neoplasias primarias de pulmón. Se caracteriza por su rapidez en la tasa de crecimiento y en el desarrollo de metástasis a distancia. Objetivos: Orientar y estandarizar el tratamiento del CPCP enfermedad extensa en México basado en evidencia clínica nacional e internacional. Material y métodos: Este documento se desarrolló como una colaboración del Instituto Nacional de Cancerología y la Sociedad Mexicana de Oncología en cumplimiento con estándares internacionales. Se integró un grupo conformado por oncólogos médicos, cirujanos oncólogos, cirujanos de tórax, radio-oncólogos y metodólogos con experiencia en revisiones sistemáticas de la literatura y guías de práctica clínica. Resultados: Se consensaron, por el método Delphi y en reuniones a distancia, las recomendaciones en CPCP enfermedad extensa, producto de preguntas de trabajo. Se identificó y evaluó la evidencia científica que responde a cada una de dichas preguntas clínicas antes de incorporarla al cuerpo de la guía. Conclusión: Esta guía proporciona recomendaciones clínicas para el manejo de la enfermedad extensa del CPCP y durante el proceso de toma de decisiones de los clínicos involucrados con su manejo en nuestro país para mejorar la calidad de la atención clínica para estos pacientes.

Abstract Background: Small cell lung cancer (SCLC) represents 13-15% of all primary lung neoplasms and is characterized by its rapid growth rate and the rapid development of distant metastases. Objectives: To guide and standardize the treatment of extensive disease small cell lung cancer in Mexico based on national and international clinical evidence. Material and methods: This document was developed as a collaboration between the National Cancer Institute and the Mexican Society of Oncology in compliance with international standards. An interdisciplinary group was formed, including medical oncologists, oncological surgeons, thoracic surgeons, radiation oncologists, and methodologists with experience in systematic reviews of the literature and clinical practice guidelines. Results: A consensus was reached, both by the Delphi method and in remote meetings, of extensive disease recommendations resulting from work questions. The scientific evidence that answers each of these clinical questions was identified and critically evaluated, before being incorporated into the body of evidence of the Guide. Conclusions: This Clinical Practice Guide provides clinical recommendations for the management of extensive disease of SCLC to contribute to the decision-making process of the clinicians involved with its management in our country, hoping that this will contribute to improving the quality of clinical care in these patients.

https://doi.org/10.24875/j.gamo.22000122

Effect of sitagliptin on down-regulation of KAT7 and SIRT1 gene expression in breast cancer cell line MCF7
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Ruteaga-Navarro, Tanya C.

; Reyes-Romero, Miguel A.

; Torres-Salazar, Quitzia L.

Cirugía y cirujanos

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jun 2023, Volume 91 N. 3 Pages 334 - 338

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Antecedentes. Hasta la fecha, el principal interés clínico de la DPP4 se centra en su inhibición en pacientes diabéticos para prolongar la vida media de las incretinas. Las alteraciones epigenéticas resultantes de la inhibición de DPP4 han sido poco exploradas. Objetivo. Determinar si la sitagliptina, un inhibidor de DPP4, tiene efectos sobre la expresión de KAT7 y SIRT1 (genes que codifican una histona acetiltransferasa y una histona desacetilasa, respectivamente) en células de cáncer de mama MCF7, que desempeñan un papel esencial en la modulación del paisaje epigenético de la cromatina. Método. Las células MCF7 se incubaron durante 20 h con sitagliptina a concentraciones de 0.5, 1.0 y 2.0 μM. Se aisló el ARN total y se determinó la expresión relativa de ARNm de KAT7 y SIRT1 mediante RT-qPCR. Resultados. Hubo una regulación a la baja en la expresión relativa de ambos genes; para KAT7, la regulación negativa alcanzó hasta 0.49 (p = 0.027) y para SIRT1 alcanzó hasta 0.55 (p = 0.037). Conclusiones. Estos resultados sugieren que la sitagliptina tiene efectos sobre el paisaje epigenético de las histonas. Este tema merece más estudios debido al uso actual de inhibidores de DPP4 en pacientes diabéticos.

Abstract Background. To date, the main clinical interest in DPP4 is focused on its inhibition in diabetic patients to prolong the half-life of incretins. Epigenetic alterations resulting from DPP4 inhibition have been poorly explored. Objective. The objective of this study was to determine, whether sitagliptin, a DPP4 inhibitor, has effects on the expression of KAT7 and SIRT1 (genes encoding a histone acetyltransferase and a histone deacetylase, respectively) in MCF7 breast cancer cells, which play an essential role in modulating the epigenetic landscape of chromatin. Material and methods. MCF7 cells were incubated for 20 h with sitagliptin at concentrations of 0.5, 1.0 and 2.0 μM. Total RNA was isolated and the relative mRNA expression of KAT7 and SIRT1 was determined by RT-qPCR. Results. There was downregulation in the relative expression of both genes; for KAT7, downregulation reached up to 0.49 (p = 0.027) and for SIRT1, it reached up to 0.55 (p = 0.037). Conclusions. These results suggest that sitagliptin has effects on the histone epigenetic landscape. This topic deserves further study due to the current sample use of DPP4 inhibitors in diabetic patients.

https://doi.org/10.24875/ciru.22000189

10.

Evolutionary model for gradual transition to Integrated Project Delivery (IPD)
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Daniel Muianga, E. Atália

; Denis Granja, A.

; Augusto Picchi, F.

; Torres Formoso, C.

Revista ingeniería de construcción

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apr 2023, Volume 38 N. 1 Pages 144 - 162

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen: Por años, los proyectos de construcción se han desarrollado y entregado usando métodos de entrega tradicionales. Sin embargo, esos métodos se han considerado ineficaces para lograr los objetivos reales del proyecto. Un modelo integrado y colaborativo, como la Entrega Integrada de Proyectos (IPD, por su sigla en inglés), un método de entrega basado en contratos relacionales ha atraído un interés creciente tanto en la academia como en la industria, pero aún escaso en Arquitectura, Ingeniería y Construcción (AIC). La mayoría de las investigaciones anteriores enfatizan en la identificación de las barreras y desafíos para implementar la IPD y pocos estudios se enfocan en desarrollar estrategias para superar esas limitaciones. Un número limitado de estudios aborda el cómo aprovechar los beneficios de la IPD en entornos AIC donde la colaboración entre las partes interesadas es deficiente. Esta investigación busca la forma de integrar la IPD en los proyectos de AIC cuando la colaboración aún es limitada. Se desarrolló un “Modelo Evolutivo de la IPD” y los lineamientos para su implementación para aplicaciones en tales ambientes. La Investigación en Ciencias del Diseño fue el enfoque adoptado en este studio para discutir y evaluar el modelo propuesto. El modelo enfatiza un proceso evolutivo y flexible para la aplicación de la IPD en proyectos en AIC. El modelo postula los elementos de IPD deseables y requeridos según los niveles de dificultad de aplicación, enfatizando la disposición de las partes interesadas para utilizarlo y la introducción gradual de la IPD en tales ambientes.

Abstract: Over the years, construction projects have been developed and delivered through traditional delivery methods. However, traditional delivery methods have been considered ineffective to achieve the real project goals. Integrated and collaborative models, such as Integrated Project Delivery (IPD), a delivery method based on relational contracts, has attracted growing interest in both academia and industry, but still scarce in Architecture Engineering, and Construction (AEC). Most prior research emphasized the identification of barriers and challenges to implementing IPD and few studies focused on developing strategies to overcome those constraints. A limited number of studies have addressed how to reap the benefits of IPD in AEC environments where collaboration between stakeholders is poor. This research investigates how to integrate IPD into AEC projects when collaboration is still limited. An “IPD Evolutionary Model” and guidelines for its implementation were developed for applications in such environments. Design Science Research was the research approach adopted in this study to discuss and evaluate the proposed model. The model emphasizes an evolutionary and flexible process for the IPD application in AEC projects. The model postulates the desirable and required IPD elements according to the levels of application difficulty, emphasising stakeholders' readiness for using it and the gradual introduction of IPD in such environments.

https://doi.org/10.7764/ric.00058.21

11.

Polyphenolic-rich extracts from Ilex paraguariensis and Larrea divaricata leaves and their antioxidant and antiCOVID-19 potential
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Contreras-Esquivel, Juan C.

; Cano-González, Carlos N.

; Ascacio-Valdes, Juan

; Aguirre-Joya, Jorge A.

; Aguillón-Gutierrez, David

; Breccia, Javier

; Espinoza-Perez, Judith D.

; Aguilar, Cristóbal N.

; Torres-León, Cristian

Biotecnia

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apr 2023, Volume 25 N. 1 Pages 61 - 66

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Las hojas de yerba mate (Ilex paraguariensis A.St. Hil) y jarilla (Larrea divaricata Cav.) se usan comúnmente como infusión de té en algunos países de América Latina. Este estudio se realizó para evaluar la actividad antioxidante (FRAP, ABTS y DPPH) y el potencial inhibitorio de los extractos de yerba mate y jarilla sobre la proteasa 3CL (Mpro) de coronavirus SARS-COV-2 por enfoque de acoplamiento molecular. Los principales compuestos bioactivos presentes en los extractos de plantas fueron identificados por HPLC-MS. De acuerdo con los resultados, los extractos polifenólicos de yerba mate y jarilla presentaron alta actividad antioxidante en los ensayos DPPH (> 91 %), ABTS (> 90 %) y FRAP (> 47 mg TE/g). Además, los compuestos fenólicos presentes en la yerba mate, quercetina-3-O-rutinósido (rutina) (-9,60 kcal/ mol) y ácido 3,4-dicafeoilquínico (- 8,20 kcal/mol) han demostrado ser más efectivos (en Mpro) que los medicamentos antivirales remdesivir y ribavirin. Los compuestos rutina y ácido 3,4-dicafeoilquínico tienen alta afinidad e interacción con uno de los residuos catalíticos Cys145 de Mpro. Estos resultados sugieren que las hojas de yerba mate y jarilla podrían potenciar las defensas antioxidantes del organismo y podrían beneficiar la salud.

Abstract Yerba mate (Ilex paraguariensis A.St. Hil) and jarilla (Larrea divaricata Cav.) leaves are commonly used as tea infusions in some Latin American countries. This study was conducted to evaluate the antioxidant activity (FRAP, ABTS, and DPPH) and the inhibitory potential of yerba mate and jarilla extracts on the 3CL protease (Mpro) from coronavirus SARS-COV-2 by a molecular docking approach. The main bioactive compounds present in the plant extracts were identified by HPLC-MS. According to the results, the extracts of yerba mate and jarilla showed high antioxidant activity in DPPH (> 91 %), ABTS (> 90 %), and FRAP (> 47 mg TE/g) assays. Additionally, the phenolic compounds present in yerba mate, quercetin-3-O-rutinoside (rutin) (-9.60 kcal/mol) and 3,4-dicaffeoylquinic acid (- 8.20 kcal/mol) were more effective on Mpro than the antiviral drugs remdesivir and ribavirin. The compounds rutin and 3,4-dicaffeoylquinic acid have a high affinity and interaction with one of the catalytic residues Cys145 of Mpro. The glycosylation of phenolic compounds affects biological activities: positively anti-COVID-19 and negatively antioxidant. The results suggest that extracts of yerba mate and jarilla leaves could enhance the body’s antioxidant defenses and can be used to improve health.

https://doi.org/10.18633/biotecnia.v25i1.1762

12.

Abdominal reconstruction in a patient with flank hernia after trauma, a case report and review of the literature
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Dávila-Portilla, Rogelio G.

; Correa-Dip, Elliott C.

; Torres-Piña, Raymundo

; Ugalde-Vitelly, Juán A.

; Guzmán-Martínez, Mario

; Jalife-Montaño, Abel

; Chapa-Azuela, Oscar

; Moreno-Hernández, Sergio G.

; Tristán-Tenorio, Carlo A.

Revista médica del Hospital General de México

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mar 2023, Volume 86 N. 1 Pages 38 - 42

Abstract: En | Text: En | PDF: En

Abstract Lateral abdominal herniae are rare, they usually occur after trauma, vascular or renal surgery, their incidence is less than 2%. Since 1906 there are just about 250 reported cases and there are many treatment options, including laparoscopic approach, direct muscle approximation with sutures, mesh, suture anchors and, even, conservative treatment. We present the case of a 30 year old male who suffers a motorcycle crash that results in a posttraumatic flank hernia. He was treated with a delayed abdominal wall reconstruction using polypropylene mesh and a fascia lata graft. There is no algorithm or specific treatment for lateral abdominal herniae and, because of its low incidence, little is known about their evolution and long-term results of the distinct reconstruction techniques. It's possible to reconstruct the abdomen wall early or delay it, depending on the abdominal cavity contamination and the patient's general condition.

https://doi.org/10.24875/hgmx.22000045

13.

Low Expression of E-Cadherin and Cdh1 Variants Associated with Diffuse Gastric Cancer
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García-Ruvalcaba, Azaria

; Vázquez-Ibarra, Katia C.

; Magaña-Torres, María T.

; C. Rizo de-la-Torre, Lourdes del

; Meléndez-Aranda, Lennon

; López-Armas, Gabriela

; Cruz-Ramos, José A.

; Peregrina-Sandoval, Jorge

; Espinoza-Jiménez, Esther

; Rosales-Gradilla, María E.

; Sánchez-López, Josefina Y.

Revista de investigación clínica

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feb 2023, Volume 75 N. 1 Pages 37 - 44

Abstract: En | Text: En | PDF: En

ABSTRACT Background Reduced or null expression of E-cadherin protein is a frequent cause of diffuse gastric cancer (DGC). More than 50% of patients with DGC present somatic variants in CDH1 gene. Objectives The objectives of this study were to study E-cadherin expression and identify variants in the CDH1 gene in gastric tumors of patients with DGC. Methods We studied 18 Mexican DGC patients who attended a hospital of the Mexican Social Security Institute; E-cadherin expression was determined by immunohistochemistry, and variants were identified by Sanger sequencing in promoter and coding regions. Predictive analysis was performed using PolyPhen-2 and HOPE software. Results We found that 56% of DGC patients showed reduced expression of E-cadherin. All patients carried CDH1 variants; overall, 12 different CDH1 variants were identified. Predictive analysis revealed that the rs114265540 variant was probably damaging, with a value of 0.985, indicating a functional impact on the E-cadherin protein. Variants rs34939176 and rs33964119 were identified as risk factors for DGC (odds' ratios [OR] = 31.3, 95% CI 6.3-154.0, p < 0.001; OR = 6.1, 95% CI 2.0-19.0, p < 0.001, respectively) given their elevated frequency and by comparing it with those reported for MXL population in the 1000 Genomes Project database. Conclusions In this Mexican population, the percentage of diffuse gastric tumors with reduced expression of E-cadherin was similar to that reported in other populations. All gastric tumors of DGC patients studied had somatic CDH1 gene variants; however, the rs114265540, rs34939176, and rs33964119 variants were importantly related to DGC.

https://doi.org/10.24875/ric.22000257

14.

Phytochemical screening of Cordia glabrata (MART.) A.DC. extracts and its potential antioxidant, photoprotective, antimicrobial and antiviral activities
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Debiasi, B. W.

; Raiser, A. L.

; Dourado, S. H. A.

; Torres, M. de P. R.

; Andrighetti, C. R.

; Bonacorsi, C.

; Battirola, L. D.

; Ribeiro, E. B.

; Valladão, D. M. S.

Brazilian Journal of Biology

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2023, Volume 83 elocation e248083

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Espécies do gênero Cordia apresentam atividades biológicas, como anti-inflamatória, analgésica, antioxidante, antiviral e antifúngica. Para a espécie Cordia glabrata (MART) A.DC., ainda não existem informações sobre seu perfil fitoquímico e possíveis atividades biológicas, deste modo, o presente estudo teve como objetivo avaliar este perfil em extratos etanólicos de folhas jovens, adultas e senescentes, bem como o potencial antioxidante, fotoprotetor, antimicrobiano e virucida. A análise fitoquímica foi realizada por CCD (Cromatografia em Camada Delgada), mostrando a presença de flavonóides, taninos e terpenos. Na avaliação por CLAE EM/EM (Cromatografia Líquida de Ultra Eficiência acoplada a Espectrometria de Massas) foi evidenciado a presença dos ácidos caféico (3,89 mgL-1), p-cumárico (6,13 mgL-1) e ferúlico (0,58 mgL-1), paralelamente, na CG/EM (Cromatografia Gasosa acoplada a Espectrometria de Massas) verificou-se maior quantidade dos ácidos graxos palmítico (51,17%), esteárico (20,34%), linoléico (9,62%) e mirístico (8,16%). Os radicais DPPH (2,2-Difenil-1-picrilhidrazil) e ABTS+ (2′-Azino-bis (ácido 3-etilbenzotiazolina-6-sulfônico)) foram utilizados para verificar o potencial antioxidante, observando-se uma atividade superior para o extrato da folha em sua fase fenológica adulta: 54,63 ± 1,06 µgmL-1 (DPPH) e 44,21 ± 1,69 mM (ABTS+). A potencial atividade fotoprotetora dos extratos foi determinada espectrofotometricamente e os valores in vitro de FPS (Fator de Proteção Solar) em folhas jovens e adultas (5,47 e 5,41 respectivamente) apresentaram valores próximos ao FPS mínimo de 6,0 exigido pela ANVISA (Agência Nacional de Vigilância Sanitária). Não foi observada atividade antimicrobiana para Staphylococcus aureus sendo a concentração inibitória mínima de 2000 μgmL-1, no entanto o ensaio anti-herpético contra o vírus Herpes simplex tipo 2 (HSV-2) mostrou uma potente atividade virucida nas concentrações testadas com um valor de CV50 <0,195 μgmL-1 e um Índice de Seletividade (IS = CC50 / CV50) maior que 448. Os resultados obtidos neste estudo sugerem que extratos de folhas de C. glabrata em seu estágio fenológico adulto apresentam potencial antioxidante, fotoprotetora e virucida, considerando os resultados de testes in vitro.

Abstract Species of the genus Cordia have shown biological activities, such as anti-inflammatory, analgesic, antioxidant, antiviral, and antifungal activities. The species Cordia glabrata (MART) A.DC. Has no information concerning its phytochemical profile and possible biological activities. Thus, this study aimed to evaluate this profile in ethanolic extracts of young, adult and senescent leaves, as well as their antioxidant, photoprotective, antimicrobial, and virucidal potentials. Phytochemical analysis was performed by TLC (thin-layer chromatography) and showed the presence of flavonoids, tannins, and terpenes. The evaluation by UPLC-MS/MS (Ultra performance liquid chromatography - tandem mass spectrometer) evidenced the presence of caffeic (3.89 mgL-1), p-cumaric (6.13 mgL-1), and ferulic (0.58 mgL-1) acids, whilst, in GC/MS (Gas chromatography–mass spectrometry) analysis there was a greater amount of palmitic (51.17%), stearic (20.34%), linoleic (9.62%), and miristic (8.16%) fatty acids. The DPPH (2,2-Diphenyl-1-picrylhydrazyl) and ABTS+ (2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radicals were used to verify the potential antioxidant activity, observing a better activity for the leaf extract in the adult phenological stage: 54.63 ± 1.06 µgmL-1 (DPPH) and 44.21 ± 1.69 mM (ABTS). The potential photoprotective activity of the extracts was determined by spectrophotometry and the in vitro values of SPF (Sun Protection Factor) in young and adult leaves (5.47 and 5.41, respectively) showed values close to the minimum SPF of 6.0 required by ANVISA (Brazilian Health Regulatory Agency). It was not observed an antimicrobial activity for Staphylococcus aureus with a minimum inhibitory concentration of 2000 μgmL-1, however the anti-herpetic assay against the Herpes simplex virus type 2 (HSV-2) showed a potent virucidal activity at the tested concentrations with CV50 value <0.195 μgmL-1 and a Selectivity Index (SI = CC50 / CV50) greater than 448. The results obtained in this study suggest that extracts of leaves of C. glabrata in their adult phenological stage have potential antioxidant, photoprotective and virucidal activity, considering in vitro test results.

https://doi.org/10.1590/1519-6984.248083 511 downloads

15.

Esferas de hidrogel relacionadas con oclusión intestinal
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Zurita-Martínez, Paulina M.

; Guerrero-Reséndiz, Diana A.

; Silva-Ramírez, Horacio

; Alcántara-Noguez, Carlos

; Lechuga-Mendoza, Luz A.

; Jiménez-Arrieta, Diana C.

; Vera-López, Isabel

; Torres-Hinojosa, Alejandra P.

Boletín médico del Hospital Infantil de México

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2023, Volume 80 Suppl. 1 Pages 64 - 68

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción: La obstrucción intestinal por ingesta accidental de cuerpos extraños se presenta muy rara vez en la edad pediátrica debido a que del 80 al 90% de los objetos pueden pasar libremente por el tracto gastrointestinal. Caso clínico: Se aborda el caso de una paciente de sexo femenino de 1 año 2 meses quien presentó obstrucción intestinal debido a la ingesta de esferas de hidrogel (poliacrilato de sodio). Dichas esferas, que son utilizadas como juguetes didácticos o sensoriales, aumentan de 200 a 400 veces su tamaño inicial mediante la absorción de agua. Se realizó radiografía abdominal en proyección decúbito supino, donde llamó la atención el hallazgo de defecto de llenado redondeado en asa de flanco derecho, ya que las esferas de hidrogel son radiolúcidas. El diagnóstico se estableció mediante ultrasonido abdominal, donde se reportó líquido libre peritoneal con datos de suboclusión por cuerpos extraños a nivel intestinal. Se indicó tratamiento conservador, encontrando persistencia de aumento de perímetro abdominal. Se realizó enterotomía y se encontraron las esferas impactadas a 30 centímetros de la válvula ileocecal. Conclusiones: Las esferas de hidrogel son peligrosas para la población pediátrica. La evolución de la paciente fue favorable debido al conocimiento del objeto extraño ingerido. Sobresale la conducta expectante que se tuvo que desempeñar debido a que se desconocía el crecimiento de las esferas y en qué momento no podrían continuar su paso por el tracto gastrointestinal.

Abstract Background: Bowel obstruction due to accidental ingestion of foreign objects occurs rarely in children because 80 to 90% of the objects can pass freely through the gastrointestinal tract. Case report: We report a case of a 14-month-old infant who presented bowel obstruction caused by the ingestion of hydrogel beads (sodium polyacrylate). Hydrogel beads are used as sensory and didactic toys that can increase their initial size 200 to 400 times by liquid absorption. An abdominal X-ray was perfomed in anteroposterior supine projection, where a round filling defect at the loop of the right flank was detected; this came to our attention because hydrogel beads are usually radiolucent. The diagnosis was established by abdominal ultrasound where free intraperitoneal fluid was reported with data of small bowel pseudo-obstruction by foreign objects. Conservative treatment was prescribed, finding persistence of increased abdominal perimeter, so an enterotomy was performed for their removal; finding impacted hydrogel beads 30 centimeters from the ileocecal valve. Conclusions: Hydrogel beads are dangerous for the pediatric population. The evolution of the patient was favorable thanks to the knowledge of the foreign objects ingested. The expectant behavior that had to be executed, stands out because we had no knowledge as to the maximum size of the hydrogel in the gastrointestinal tract.

https://doi.org/10.24875/bmhim.22000137

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