ABSTRACT Sugarcane-duck symbiosis (SDS) is an ecological breeding production method wherein meat ducks are released in sugarcane (Saccharum officinarum L.) fields during the middle and late stages of sugarcane growth. The effects of SDS on sugarcane rhizobacterial diversity remain poorly understood. We conducted field experiments with sugarcane monoculture (SC) and SDS and performed genetic analyses on the rhizobacterial communities based on 16S rRNA across different growth stages of sugarcane. The effects of soil physicochemical factors on the bacterial community structure were analyzed via redundancy analysis. We found that soil organic matter (SOM), total N (TN), nitrate N (NN), and ammonium N (AN) contents of sugarcane rhizosphere soils increased during different growth periods in SDS. During the perennial root stages, the Shannon-, Ace-, and Chao1 indices increased, whereas the available P (AP) content decreased. The soil pH was more balanced and its effect on the soil bacterial community was insignificant. The dominant sugarcane rhizosphere flora were Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, Firmicutes, norank_f__norank_o__Gaiellales, Sphingomonas, norank_f__Roseiflexaceae, Bacillus, and norank_f__Xanthobacteraceae, but with significant inter-annual variations. Redundancy analysis showed that SOM, TN, NN, AN, AP, catalase (CAT) and invertase (INV) activity were closely associated with the diversity of the soil bacterial communities, and TN and INV activity were the main drivers of sugarcane rhizobacterial communities in the SDS groups. In conclusion, SDS improves the fertilizer supply capacity and soil quality of sugarcane soils by regulating major soil nutrient content, available nutrients, and microbial diversity. This study provides a theoretical basis for scientifically managing SDS and the screening of biocontrol bacteria.

Abstract Objective: Periventricular-intraventricular hemorrhage is the most common type of intracranial bleeding in newborns, especially in the first 3 days after birth. Severe periventricular-intraventricular hemorrhage is considered a progression from mild periventricular-intraventricular hemorrhage and is often closely associated with severe neurological sequelae. However, no specific indicators are available to predict the progression from mild to severe periventricular-intraventricular in early admission. This study aims to establish an early diagnostic prediction model for severe PIVH. Method: This study was a retrospective cohort study with data collected from the MIMIC-III (v1.4) database. Laboratory and clinical data collected within the first 24 h of NICU admission have been used as variables for both univariate and multivariate logistic regression analyses to construct a nomogram-based early prediction model for severe periventricular-intraventricular hemorrhage and subsequently validated. Results: A predictive model was established and represented by a nomogram, it comprised three variables: output, lowest platelet count and use of vasoactive drugs within 24 h of NICU admission. The model’s predictive performance showed by the calculated area under the curve was 0.792, indicating good discriminatory power. The calibration plot demonstrated good calibration between observed and predicted outcomes, and the Hosmer-Lemeshow test showed high consistency (p = 0.990). Internal validation showed the calculated area under a curve of 0.788. Conclusions: This severe PIVH predictive model, established by three easily obtainable indicators within the NICU, demonstrated good predictive ability. It offered a more user-friendly and convenient option for neonatologists. Objective Periventricularintraventricular Periventricular intraventricular newborns birth periventricularintraventricular periventricular sequelae However Method MIMICIII MIMIC III v1.4 v14 v v1 4 (v1.4 database 2 nomogrambased nomogram based validated Results output models s 0792 0 792 0.792 power outcomes HosmerLemeshow Hosmer Lemeshow p 0.990. 0990 0.990 . 990 0.990) 0788 788 0.788 Conclusions ability userfriendly user friendly neonatologists v1. (v1. 079 79 0.79 099 0.99 99 078 78 0.78 (v1 07 7 0.7 09 0.9 9 (v 0.

Higher Himalayan catchments are often poorly monitored for hydrological activities involving flood flow prediction for the safety of riverside communities and the successful operation of hydropower projects. This study aimed to estimate the comparative performance of artificial neural network (ANN) based flow prediction models using 10 years of daily river flow data of Kaligandaki catchment at Kotagaun, Nepal, which is a snow-fed catchment in the Himalayan region. The flow prediction models were trained and tested at a hydrological station using the previous 3 days' river flow data to predict the 1-day ahead flow data. Eight different training functions were employed in an ANN model for comprehensive statistical assessment of accuracy and precision of each training function. The most significant and validated result obtained in this study is the comprehensive comparison of various training functions' performance, and identification )f the most efficient training function for the study case. Among the training functions investigated, the Levenberg-Marquardt backpropagation function exhibits the best performance for the model having Nash-Sutcliffe efficiency, root mean square error and mean absolute error values of 0.866, 209.578 and 75.422, respectively. This study provides a fundamental basis for accurate flow prediction of topographically challenged catchments where hydrological monitoring and data collection may be limited. In particular, r his model will help to improve early warning system, hydrological planning, and the safety of riverside communities in the Himalayan region.

Abstract Background In a recent genome-wide association study, novel genetic variations of WNT9A were reported to be involved in the etiopathogenesis of thumb osteoarthritis (TOA) in Caucasians. Our purposes were to replicate the association of WNT9A with the development of TOA in the Chinese population and to further unveil the functional role of the risk variants. Methods SNP rs11588850 of WNT9A were genotyped in 953 TOA patients and 1124 healthy controls. The differences of genotype and allele distributions between the patients and healthy controls were evaluated using the Chi-square test. Luciferase Reporter Assay was performed to investigate the influence of variant on the gene expression. Results There was significantly lower frequency of genotype AA in TOA patients than in the controls 74.9% vs. 81.9%, p < 0.001). The frequency of allele A was remarkably lower in the patients than in the controls (86.3% vs. 90.5%, p < 0.001), with an odds ratio of 0.66 (95% CI = 0.54–0.80). Luciferase Reporter Assay showed that the construct containing mutant allele G of rs11588850 displayed 29.1% higher enhancer activity than the wild allele A construct (p < 0.05). Conclusions Allele G of rs11588850 was associated with the increased risk of TOA possibly via up-regulation of WNT9A expression. Further functional analysis into the regulatory role of rs11588850 in WNT9A expression can shed new light on the genetic architecture of TOA. Key Points Genetic variants of WNT9A were associated with the incidence and severity of TOA. Allele G of rs11588850 was associated with an increased transcriptional activity of WNT9A promoter. Allele G of rs11588850 may add to the risk of TOA possibly via up-regulation of WNT9A expression. Further functional analysis into the regulatory role of rs11588850 in WNT9A expression can shed new light on the genetic architecture of TOA. genomewide genome wide study WNTA WNT (TOA Caucasians rs rs1158885 95 112 Chisquare Chi square test 749 74 9 74.9 vs 819 81 81.9% 0.001. 0001 0.001 . 0 001 0.001) 86.3% 863 86 3 (86.3 905 90 5 90.5% 0.001, , 066 66 0.6 95% (95 0.54–0.80. 054080 0.54–0.80 54 80 0.54–0.80) 291 29 1 29.1 0.05. 005 0.05 05 0.05) upregulation up regulation promoter rs115888 11 7 74. 8 81.9 000 0.00 00 86.3 (86. 90.5 06 6 0. (9 05408 0.54–0.8 2 29. 0.0 rs11588 81. 86. (86 90. ( 0540 0.54–0. rs1158 (8 054 0.54–0 rs115 0.54– rs11 0.54 rs1 0.5

High-altitude hypoxia exposure can lead to phospholipase D-mediated lipid metabolism disorder in spleen tissues and induce ferroptosis. Nonetheless, the key genes underlying hypoxia-induced splenic phospholipase D and the ferroptosis pathway remain unclear. This study aimed to establish a hypoxia animal model. Combined transcriptomic and proteomic analyses showed that 95 predicted target genes (proteins) were significantly differentially expressed under hypoxic conditions. Key genes in phospholipase D and ferroptosis pathways under hypoxic exposure were identified by combining Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis techniques. Gene set enrichment analysis (GSEA) showed that the differential gene sets of the phospholipase D and ferroptosis signaling pathways were upregulated in the high-altitude hypoxia group. The genes in the phospholipase D signalling pathway were verified, and the expression levels of KIT and DGKG were upregulated in spleen tissues under hypoxic exposure. Subsequently, the mRNA and protein expression levels of genes from the exogenous pathway such as TFRC, SLC40A1, SLC7A11, TRP53, and FTH1 and those from the endogenous pathway such as GPX4, HMOX1, and ALOX15 differentials in the ferroptosis signalling pathway were verified, and the results indicated significant differential expression. In summary, exposure to high-altitude hypoxia mediated phospholipid metabolism disturbance through the phospholipase D signalling pathway and further induced ferroptosis, leading to splenic injury. Highaltitude High altitude Dmediated Nonetheless hypoxiainduced unclear model 9 proteins (proteins conditions GO (GO KEGG (KEGG techniques GSEA (GSEA highaltitude high group verified Subsequently TFRC SLC40A1 SLCA SLC A SLC7A11 TRP53 TRP FTH GPX4 GPX HMOX1 HMOX ALOX ALOX1 summary injury SLC40A SLC7A1 TRP5 SLC7A

Abstract Background The development of rosacea is suggested to be closely associated with lipid metabolism, inflammation, and anxiety/depression. Gamma linolenic acid (GLA) is a key factor participating in lipid metabolism, which is also confirmed to regulate the inflammatory response. However, the associations of serum GLA levels with rosacea severity and psychological status still remain unclear. Objective and limitations of the study The present study aimed to investigate the associations of gamma linolenic acid (GLA), a key factor participating in lipid metabolism and the inflammatory response, with rosacea severity and psychological status. The present study still had some limitations. First, this study is a cross-sectional study and does not provide longitudinal evidence about the relationship between GLA and rosacea; Second, the cohort in this study is also relatively small, and a larger cohort is needed in further investigation to reveal the potential role of lipid metabolism in the pathogenesis of rosacea. Methods A total of 62 rosacea patients were consecutively recruited. Patient’s Self-Assessment (PSA) scale and Clinician Erythema Assessment (CEA) as well as 7-item Generalized Anxiety Disorder (GAD-7) and 9-item Patient Health Questionnaire (PHQ-9) were conducted to evaluate the degree of erythema severity and anxiety/depression, respectively. Serum GLA levels were determined by gas chromatography mass. Results Lower levels of serum GLA in rosacea patients were observed (p < 0.001), and subgroup analysis revealed that patients with higher-level GLA had lower scores of PSA, CEA, GAD-7 and PHQ-9. Moreover, Spearman correlation analysis uncovered that serum GLA levels were negatively associated with PSA, CEA, GAD-7 as well and PHQ-9 scores, respectively. Linear regression model found that serum GLA levels at baseline were a predictive factor for prognosis of clinical outcomes after 1-month conventional treatment. Conclusion The present study indicates that lower levels of serum GLA in rosacea patients are negatively associated with the degree of erythema and anxiety/depression status. inflammation anxietydepression anxiety depression (GLA response However unclear GLA, , First crosssectional cross sectional Second small 6 recruited Patients s SelfAssessment Self PSA (PSA CEA (CEA 7item item 7 GAD7 GAD (GAD-7 9item 9 PHQ9 PHQ (PHQ-9 respectively mass p 0.001, 0001 0.001 0 001 0.001) higherlevel higher level GAD- PHQ9. 9. Moreover PHQ- 1month month 1 treatment (GAD- (PHQ- 000 0.00 00 (GAD (PHQ 0.0 0.

Abstract Objectives Nasopharyngeal carcinoma (NPC) is an aggressive epithelial cancer. The expression of miR-186 is decreased in a variety of malignancies and can promote the invasion and metastasis of cancer cells. This study aimed to explore the role and possible mechanism of miR-186 in the metastasis and epithelial-mesenchymal transformation (EMT) of NPC. Methods The expression of miR-186 in NPC tissues and cells was detected by RT-PCR. Then, miR-186 mimic was used to transfect NPC cell lines C666-1 and CNE-2, and cell activity, invasion and migration were detected by CCK8, transwell and scratch assay, respectively. The expression of EMT-related proteins was analyzed by western blotting analysis. The binding relationship between miR-186 and target gene Zinc Finger E-Box Binding Homeobox 1 (ZEB1) was confirmed by double luciferase assay. Results The expression of miR-186 in NPC was significantly decreased, and transfection of miR-186 mimic could significantly inhibit the cell activity, invasion, and migration, and regulate the protein expressions of E-cadherin, N-cadherin and vimentin in C666-1 and CNE-2 cells. Further experiments confirmed that miR-186 could directly target ZEB1 and negatively regulate its expression. In addition, ZEB1 has been confirmed to be highly expressed in NPC, and inhibition of ZEB1 could inhibit the activity, invasion, metastasis and EMT of NPC cells. And co-transfection of miR-186 mimic and si-ZEB1 could further inhibit the proliferation and metastasis of NPC. Conclusion miR-186 may inhibit the proliferation, metastasis and EMT of NPC by targeting ZEB1, and the miR-186/ZEB1 axis plays an important role in NPC. (NPC miR186 miR 186 miR-18 epithelialmesenchymal mesenchymal (EMT RTPCR. RTPCR RT PCR. PCR RT-PCR Then C6661 C C666 C666- CNE2, CNE2 CNE 2, 2 activity CCK8 CCK assay respectively EMTrelated related analysis EBox E Box ZEB (ZEB1 Ecadherin, Ecadherin cadherin, cadherin E-cadherin Ncadherin N CNE- addition cotransfection co siZEB1 siZEB si si-ZEB miR186/ZEB1 miR186ZEB1 miRZEB 186/ZEB1 miR-186/ZEB miR18 18 miR-1 C66 (ZEB miR186/ZEB miR186ZEB 186ZEB1 186/ZEB miR1 miR- C6 186ZEB

Abstract The imbalance between pro-inflammatory M1 and anti-inflammatory M2 macrophages plays a critical role in the pathogenesis of sepsis-induced acute lung injury (ALI). Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) may modulate macrophage polarization toward the M2 phenotype by altering mitochondrial activity. This study aimed to investigate the role of the PGC-1α agonist pioglitazone (PGZ) in modulating sepsis-induced ALI. A mouse model of sepsis-induced ALI was established using cecal ligation and puncture (CLP). An in vitro model was created by stimulating MH-S cells with lipopolysaccharide (LPS). qRT-PCR was used to measure mRNA levels of M1 markers iNOS and MHC-II and M2 markers Arg1 and CD206 to evaluate macrophage polarization. Western blotting detected expression of peroxisome proliferator-activated receptor gamma (PPARγ) PGC-1α, and mitochondrial biogenesis proteins NRF1, NRF2, and mtTFA. To assess mitochondrial content and function, reactive oxygen species levels were detected by dihydroethidium staining, and mitochondrial DNA copy number was measured by qRT-PCR. In the CLP-induced ALI mouse model, lung tissues exhibited reduced PGC-1α expression. PGZ treatment rescued PGC-1α expression and alleviated lung injury, as evidenced by decreased lung wet-to-dry weight ratio, pro-inflammatory cytokine secretion (tumor necrosis factor-α, interleukin-1β, interleukin-6), and enhanced M2 macrophage polarization. Mechanistic investigations revealed that PGZ activated the PPARγ/PGC-1α/mitochondrial protection pathway to prevent sepsis-induced ALI by inhibiting M1 macrophage polarization. These results may provide new insights and evidence for developing PGZ as a potential ALI therapy. proinflammatory pro inflammatory M antiinflammatory anti sepsisinduced sepsis induced . (ALI) proliferatoractivated proliferator 1alpha alpha 1 PGC1α PGCα PGC 1α α (PGC-1α activity (PGZ CLP. CLP (CLP) MHS MH S LPS. LPS (LPS) qRTPCR qRT PCR MHCII MHC II Arg CD CD20 PPARγ (PPARγ PGC1α, 1α, NRF1 NRF NRF2 mtTFA function staining qRTPCR. PCR. CLPinduced wettodry wet dry ratio tumor factorα, factorα factor α, factor-α interleukin1β, interleukin1β interleukinβ interleukin 1β, 1β β interleukin-1β interleukin6, interleukin6 6 , interleukin-6) PPARγ/PGC1α/mitochondrial PPARγPGC1αmitochondrial PPARγPGCαmitochondrial PPARγ/PGC 1α/mitochondrial therapy (ALI (CLP (LPS CD2 interleukin-6 PPARγPGC αmitochondrial 1αmitochondrial interleukin-

SUMMARY OBJECTIVE: The objective of this study was to explore the relationship between serum soluble fms-like tyrosine kinase 1 and the severity of acute pancreatitis and its diagnostic utility. METHODS: This study was carried out by searching Chinese and English literature from the establishment of the database to July 9, 2023, systematically, and assessing the quality and heterogeneity of the articles included. RESULTS: Thirteen studies with a total of 986 patients were included. Patients with severe acute pancreatitis showed higher levels of soluble fms-like tyrosine kinase 1 compared with mild acute pancreatitis [weighted mean difference=76.64 pg/mL, 95% confidence interval (95%CI 50.39–102.89, p<0.001)]. Soluble fms-like tyrosine kinase 1 predicted pooled sensitivity, specificity, and area under the curve were 79%, 74%, and 0.85 for severe acute pancreatitis, with some heterogeneity (I2>50% or p<0.05). In the subgroup analysis, cutoff >150 pg/mL was found to be a heterogeneous factor. CONCLUSION: Soluble fms-like tyrosine kinase 1 is a reliable tool for identifying acute pancreatitis severity, but only as a screening tool. OBJECTIVE fmslike fms like utility METHODS 9 2023 systematically included RESULTS 98 weighted difference7664 difference 76 64 difference=76.6 pgmL pg mL 95 95%CI 95CI CI 503910289 50 39 102 89 50.39–102.89 p<0.001. p0001 p p<0.001 . 0 001 p<0.001)] sensitivity specificity 79 79% 74 74% 085 85 0.8 I2>50% I250 I I2 (I2>50 p<0.05. p005 p<0.05 05 p<0.05) analysis 150 >15 factor CONCLUSION 202 difference766 7 6 difference=76. 50391028 5 3 10 8 50.39–102.8 p000 p<0.00 00 p<0.001) 08 0. I2>50 I25 (I2>5 p00 p<0.0 15 >1 20 difference76 difference=76 5039102 50.39–102. I2>5 (I2> p0 p<0. > 2 difference7 difference=7 503910 50.39–102 I2> (I2 p<0 difference= 50391 50.39–10 (I p< 5039 50.39–1 503 50.39– 50.39 50.3 50.

Abstract Background In contemporary society, with the accelerated pace of work and life, more and more people feel different degrees of stress. Long-term stress may not only lead to insomnia, but also to mental health problems (e.g., anxiety and depression), which has a significant impact on people's quality of life and mental health. Objective This study primarily investigates the mechanism through which stress affects sleep quality among college students. Methods We conducted research on 1653 Chinese college students using four scales with high reliability and validity: stress, the Pittsburgh Sleep Quality Index, social anxiety, and rumination. Results The study found: (1) Stress can significantly and positively predict sleep quality and rumination; (2) Rumination can positively predict social anxiety; (3) Social anxiety can positively predict sleep quality; (4) Stress can affect sleep quality through social anxiety and rumination separately, and stress can also affect sleep quality through the chained mediation of rumination and social anxiety. Conclusion This study reveals the relationship and mechanisms between stress and sleep quality. It not only deepens the research on the impact of stress on sleep quality but also provides theoretical support and new methods for mental health professionals to help clients improve their sleep quality. In practice, in addition to using some common psychological intervention methods to help individuals reduce stress, we should pay more attention to how to help clients reduce rumination and social anxiety, This is significant in improving the quality of an individual's sleep. society Longterm Long term insomnia e.g., eg e g (e.g. depression, depression , depression) peoples s 165 validity Index found 1 (1 2 (2 3 (3 4 (4 separately practice individual e.g. (e.g 16 ( e.g

ABSTRACT During the harvesting of waste straw in the field, a self-propelled baler undergoes significant oscillations in the longitudinal direction around its centre of gravity, which greatly affect the stability of operation and the comfort of the driver. In order to analyse the vibration characteristics of the main frame under multi-source excitation, the complete frame is divided into a chassis frame and a baling frame. A nine-degree-of-freedom vibration system model of the whole machine is established based on the relationship between the internal motion devices, and simulations are carried out using MATLAB/Simulink. In addition, a multi-body dynamics simulation of the compression device is carried out, and the law governing the variation in force at different unit points of the mechanism is analysed. Finally, the vibration response of the main frame under different working conditions is explored. The results show that the main frequency component of the frame in the no-load state is 80 Hz, and the main response frequency of the frame is about 1.6 Hz, a finding that is consistent with the operating frequency of the compression mechanism. In particular, the displacement amplitude of the simulated signal at the centroid position is consistent with that measured in experiments, which verifies the validity of the dynamic model. Our results can provide guidance for future work aiming to further reduce the body vibration amplitude and optimise the design of the frame. field selfpropelled self propelled gravity driver multisource multi source excitation ninedegreeoffreedom nine degree freedom devices MATLABSimulink MATLAB Simulink MATLAB/Simulink addition multibody analysed Finally explored noload no load 8 Hz 16 1 6 1. particular experiments

Abstract Objective This study aimed to explore the effects of Apatinib combined with Temozolomide (TMZ) on the levels of Soluble PD-1 (sPD-1) and Soluble Programmed Death-1 Ligand (sPD-L1) in patients with drug-resistant recurrent Glioblastoma (GB). Study design A total of 69 patients with recurrent GB from September 2020 to March 2022 were recruited and assigned to the control group (n = 34) and observation group (n = 35) according to different treatment options after tumor recurrence. The control group was treated with TMZ, and the observation group was treated with Apatinib combined with TMZ. Levels of sPD-1 and spd-l1, clinical efficacy, survival time and adverse reactions were observed and compared between the two groups. Results General data including gender, age, body mass index, and combined diseases indicated no statistical significance between groups (p > 0.05). Before the intervention, sPD-1 and sPD-L1 levels were not significantly different in the two groups (p > 0.05). After interventions, levels of PD-1 and sPD-L1 levels decreased significantly (p < 0.05). The objective remission rate and clinical benefit rate of the observation group were higher and overall survival and progression-free survival were longer than those of the control group (p < 0.05). No significant difference was observed in major adverse reactions among patients (p > 0.05). Conclusions Apatinib combined with TMZ is safe and effective in the treatment of recurrent GB. The combined application of the two can reduce the levels of sPD-1 and sPD-L1, which has important clinical application value. (TMZ PD1 PD 1 PD- sPD1 sPD (sPD-1 Death1 Death Death- sPDL1 sPDL L1 L (sPD-L1 drugresistant drug resistant . (GB) 6 202 n 34 35 recurrence sPD- spdl1, spdl1 spdl spd l1, l1 l spd-l1 efficacy gender age index p 0.05. 005 0.05 0 05 0.05) intervention sPD-L interventions progressionfree progression free sPDL1, L1, value (sPD- (sPD-L (GB 20 3 spd-l 00 0.0 (sPD 2 0.

ABSTRACT Broccoli is increasingly favored by consumers as it is rich in vitamins and many other bioactive compounds. However, existing large tractor-drawn vegetable harvesting machinery is more suitable for large farmland and farm cultivation patterns. Therefore, this paper develops tracked broccoli harvesting equipment for small farmland and tests the ability to harvest broccoli. The tracked broccoli harvester developed in this paper is capable of traveling on its own in the field, cutting the stalks and transporting the broccoli bulbs. This paper analyses the working process of the cutting device and designs the working parameters. Through the cutting bench experiment, the optimal working parameters of the cutting device were determined as 456 r/min of rotational speed, 8.312 mm of overlap, and 0.255 m/s of pusher speed. In the field test, the broccoli leakage rate was 4.8%, the cutting qualification rate was 81.6%, the transport qualification rate was 95.62%, the damage rate was 3.96%, and the total loss rate was 8.55%. This structural model is an important technical support for broccoli harvesting equipment in China. compounds However tractordrawn tractor drawn patterns Therefore bulbs experiment 45 rmin r min speed 8312 8 312 8.31 overlap 0255 0 255 0.25 ms m s test 48 4 4.8% 816 81 6 81.6% 9562 95 62 95.62% 396 3 96 3.96% 855 55 8.55% China 831 31 8.3 025 25 0.2 4.8 81.6 956 9 95.62 39 3.96 85 5 8.55 83 8. 02 2 0. 4. 81. 95.6 3.9 8.5 95. 3.