ABSTRACT Background: Cervical cancer (CC), as a serious menace to the health of women, has long been one of the most lethal gynecologic neoplasms throughout the world. Long non-coding RNA (LncRNA) NR2F1-AS1 has been documented to exert crucial functions in many malignant tumors. Nonetheless, the function and molecular mechanism of NR2F1-AS1 in CC remain completely unknown. Objective: This study aimed to explore the function and molecular mechanism of NR2F1-AS1 in CC. Methods: The expression levels of NR2F1-AS1, miR-642a-3p, NR2F1 in CC tissues, and cell lines were examined by reverse transcription real-time quantitative polymerase chain reaction. Cell viability, proliferation, migration, and invasion were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, colony formation and Transwell assays. The protein levels of epithelial-mesenchymal transition markers and NR2F1 in CC cells were assessed by Western blot analysis. The correlations among NR2F1-AS1, miR-642a-3p, and NR2F1 were estimated through luciferase reporter and RNA immunoprecipitation assays. Results: NR2F1-AS1 expression was clearly downregulated in CC tissues and cell lines. Molecular mechanistic experiments showed that NR2F1-AS1 overexpression upregulated NR2F1 expression in CC cells by directly binding to miR-642a-3p, and inhibiting by this way cell viability, proliferation, migration, and invasion in CC. Rescue assays showed that NR2F1 knockdown or miR-642a-3p overexpression offset NR2F1-AS1 upregulation-induced inhibition on CC cell malignant phenotypes. Conclusion: These findings revealed that NR2F1-AS1 played a tumor suppressor role in CC by mediating the miR-642a-3p/NR2F1 axis.

ABSTRACT: The impacts of continuous cropping of banana on soil microbiological and biochemical properties are little understood. In this study, we evaluated the variations in soil bacterial community abundance and diversity, microbial biomass carbon (MBC) and nitrogen (MBN) as well as soil enzyme activities involved in C, N and P cycles as affected by continuous cropping of banana. An initial increase in bacterial 16S rRNA copy and soil microbial biomass was observed in the second cropping and then decreased until the fourth cropping. The diversity of bacterial community showed a continuous decrease throughout the experiment. In addition, continuous cropping of banana caused shifts in bacterial community composition and structures. Soil urease and invertase exhibited the highest activities in the second cropping and then decreased gradually from the second to the fourth cropping. The phosphatase activity showed a gradual increase from the first to the third cropping. The bacterial 16S rRNA copy was positively correlated with the contents of MBN and urease activities. The results indicated that continuous cropping of banana was responsible for the disturbance of the bacterial community and that the effect on enzyme activity varies depending on the type of soil enzyme.