ABSTRACT Purpose: The objective of this study was to evaluate whether morphological (linear measurements) and functional (ADC value) assessments of periprostatic fat can predict the aggressiveness of prostate cancer (PCa) over a 5-year follow-up period. Material and Methods: This retrospective study included patients with histologically proven PCa who underwent 3.0T MRI between July 2016 and June 2018. Clinical and demographic data collected included PSA, PSA density (dPSA), ISUP grade, clinical and pathological staging, and treatment details. MRI-derived parameters were assessed by an experienced radiologist, who measured subcutaneous and periprostatic fat thickness, and calculated ADC values from ROI plots in periprostatic fat. Clinical and MRI parameters were analyzed for associations with biochemical recurrence, systemic metastasis, and PCa-related mortality. Results: After applying exclusion criteria, 109 patients were included. Using the Cox model, dPSA (p<0.01), systemic disease at diagnosis (p<0.01), and mean ADC (p<0.02) were independent predictors of overall survival (OS). For progression-free survival (PFS), only dPSA (p<0.01) and systemic disease at diagnosis (p<0.01) were significant predictors. In the Poisson Model for systemic recurrence risk, dPSA had a relative risk (RR) of 1.04 (95%CI 1.0-1.07, p=0.03), systemic disease at diagnosis had an RR of 63.3 (95%CI 3.7-86.4, p<0.01), and average ADC had an RR of 3.42 (95%CI 1.52-7.69, p<0.01). Conclusions: The ADC value of periprostatic fat may serve as an additional tool for PCa risk stratification, correlating with poorer outcomes such as systemic recurrence and overall survival. If validated by external, prospective, multicenter studies, these findings could impact future therapeutic decisions.

This paper presents an analytical multiresidue method for the quantification of 7 nitroimidazoles, 19 anthelmintics, 3 amphenicols, and 4 dyes in poultry, pork, beef, horse, shrimp, and fish samples by isotope dilution ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) in a single 6 min run. Samples were prepared using a modified quick, easy, cheap, effective, rugged and safe (QuEChERS)-type extraction with ethyl acetate and Na2SO4-NaCl (2:1), concentration into dimethyl sulfoxide (DMSO), and defatting by liquid-liquid extraction (LLE) with n-hexane. No clean-up using solid-phase extraction (SPE) cartridges or dispersive solid-phase extraction (DSPE) was necessary. The linearity ranges (in μg kg-1) were 0.10-0.60 for chloramphenicol, 0.50-3.0 for dyes, 1.5-9.0 for nitroimidazoles, and 5.0-30.0 for the other drugs. The limits of quantitation (LOQ) were established at the lowest calibration curve levels. Except for chloramphenicol in the horse matrix, analyte recovery and reproducibility were satisfactory (recovery (fREC): 75.0 to 116.6%; coefficient of variation (CV): 1.2 to 29.9%). For the unapproved substances and the substances with a maximum residue level (MRL), respectively, the decision limits (CCα) were 0.14-4.10 and 5.28-1003.60 μg kg-1, while the detection capabilities (CCβ) were 0.13-3.33 and 5.46-11.56 μg kg-1.

This work aims to analyze the effects of corrosion in different municipalities through the exposure of 1020 carbon-steel plates to the atmosphere and buried in soil. Sea air and pollution were treated as major corrosion agents and zinc has been tested to prove its effectiveness as a sacrificial anode. For this analysis, weighing and spectrophotometric methods were used to determine the iron mass loss after five months of exposure. From the data obtained, corrosion rates were calculated and compared based on different regional conditions. The results showed that the coastal region of the state of São Paulo presented higher corrosion rates compared to places of higher altitudes and further away from the sea, indicating that sea air and pollution are important agents in catalyzing the deterioration processes. In addition, zinc was efficient as a sacrificial anode, demonstrating its potential application as corrosion protection in 1020 carbon-steel plates.

ABSTRACT Given the large differences in the results of inoculation and co-inoculation in the bean crop, an experiment in cultivating winter beans was carried out in 2019 under greenhouse conditions with the aim of evaluating the performance of the beans and a possible increase in plant growth and grain production, using seeds inoculated or co-inoculated with Rhizobium tropici and Azospirillum brasilense, with and without fungicide and/or insecticide, in sandy and clayey soils. A total of 112 pots were used, and 14 treatments were applied in a completely randomised experimental design, with four replications. The Scott-Knott test was used to analyse the results, and showed that whether using untreated seeds or carrying out complete chemical treatment with fungicide and insecticide plus co-inoculation with R. tropici and A. brasilense, winter bean production was reduced in clayey soil, while the joint use of fungicide and insecticide, fungicide and R. tropici, or fungicide, insecticide and R. tropici, favoured shoot development. Inoculating with A. brasilense, without R. tropici and with insecticide, or insecticide and fungicide, favoured greater root growth in clayey soil. In sandy soil, grain production was higher using seeds treated solely with insecticide, solely with R. tropici, or with a combination of both. Production was lower in the joint treatment with fungicide, insecticide and the co-inoculation of bacteria, or when using insecticide together with A. brasilense.

ABSTRACT Objective: The objective of this study was to analyze suicide attempts in adolescents treated in the emergency department before and during the coronavirus disease 2019 pandemic. Methods: This cross-sectional, documentary, and retrospective study assessed the medical records of adolescents treated for suicide attempts in two emergency rooms linked to a teaching hospital in São Paulo, Brazil. Data were collected from the pre-pandemic period (between January 1, 2015, and March 10, 2020) and the pandemic period (between March 11, 2020, and May 5, 2023), characterizing the adolescents, risk factors for suicide attempts during the pandemic, and treatment. Data were subjected to descriptive and inferential analyses, including association tests and trend analysis, with a p-value of <0.05 considered statistically significant. The national ethical guidelines were followed. Results: Data on 140 suicide attempts were collected, of which 54 occurred during the pandemic. The trend in these cases remained stable (p>0.05). We observed an association between adolescents who had completed high school and cases during the pandemic, with a previous mental disorder diagnosis and pre-pandemic cases, and a higher number of suicide attempt notifications to the Brazilian epidemiological surveillance system during the pandemic period (p<0.05). Feelings of loneliness were the most prevalent risk factor during the pandemic. Conclusions: The COVID-19 pandemic did not show an influence on the number of suicide attempt cases in adolescents. However, it was observed that the cases during this period were not related to previous mental disorder diagnoses, as seen in the pre-pandemic period, which may suggest that other risk factors were associated with suicide attempt cases during the pandemic.

RESUMO Objetivo: Analisar as tentativas de suicídio em adolescentes atendidos em um departamento de urgência e emergência antes da pandemia da COVID-19 e durante seu curso. Métodos: Estudo transversal, documental e retrospectivo, conduzido com prontuários de adolescentes atendidos por tentativa de suicídio, em dois prontos-socorros vinculados a um hospital escola no município de São Paulo, Brasil. Foram coletados dados do período pré-pandêmico (entre 1o de janeiro de 2015 e 10 de março de 2020) e período pandêmico (entre 11 de março de 2020 e 5 de maio de 2023), com a caracterização do adolescente, dos fatores de risco à tentativa de suicídio na pandemia e do atendimento. Os dados foram submetidos a análise descritiva e inferencial, com testes de associação e análise de tendência, considerando-se o valor de p<0,05 como diferença estatística. Respeitaram-se as diretrizes éticas nacionais. Resultados: Foram coletados dados de 140 atendimentos de tentativa de suicídio, dos quais 54 ocorreram no período pandêmico. A tendência de atendimentos mostrou-se estacionária (p>0,05). Observou-se associação entre os adolescentes que estudaram até o ensino médio e os atendimentos na pandemia; ter um diagnóstico de transtorno mental prévio e o atendimento pré-pandêmico; e o maior número de notificações de tentativa de suicídio para o sistema de vigilância epidemiológico brasileiro no período pandêmico (p<0,05). A sensação de solidão destacou-se como fator de risco pandêmico mais prevalente. Conclusões: A pandemia da COVID-19 não mostrou influência nos atendimentos de tentativa de suicídio em adolescentes. Todavia, observou-se que os casos neste período não tiveram relação com o diagnóstico de transtorno mental prévio, como visto no período pré-pandêmico, o que pode sugerir que outros fatores de risco estiveram associados aos casos de tentativa de suicídio durante a pandemia.

ABSTRACT The cultivation of conventional maize in refuge areas is important for preserving Bt technology and serving specific markets with greater added value to the grains. Therefore, research aimed at chemical weed control must also continue to be directed to conventional maize hybrids. The objective was to evaluate the selectivity of herbicides applied in preand post-emergence of a conventional maize hybrid cultivated in the Cerrado biome of Brazil. Two field experiments were set up, one in summer and the other in second season. The treatments were composed of eleven herbicide treatments, whose doses are presented in g ha-1 of active ingredient: S-metolachlor (1,440), S-metolachlor (1,680), mesotrione + atrazine (115.2 + 2,000), mesotrione + atrazine (192 + 2,000), tembotrione + atrazine (75.6 + 2,000), tembotrione + atrazine (100.8 + 2,000), nicosulfuron + atrazine (16 + 2,000), nicosulfuron + atrazine (24 + 2,000), [mesotrione + atrazine] ([120 + 1,200]), sequential application of [mesotrione + atrazine] ([60 + 600]), atrazine (2,000), plus a weeded control. Regardless of the experiment, all herbicides applied preand post-emergence of maize led to low percentages of phytointoxication. In both experiments, none of the treatments caused reductions in crop stand, confirming the absence of plant mortality due to the application of herbicides. Plant tipping/lodging was seen in summer maize, with no treatment effect. Maize 100grain mass and yield did not change as a result of the application of herbicides in preand post-emergence of the crop. All herbicide treatments evaluated showed selectivity for the conventional maize hybrid.

RESUMO O cultivo do milho convencional em áreas de refúgio é importante para preservação da tecnologia Bt e para atender mercados específicos com maior valor agregado aos grãos. Portanto, as pesquisas voltadas ao controle químico de plantas daninhas também devem continuar direcionadas aos híbridos convencionais de milho. O objetivo foi avaliar a seletividade de herbicidas aplicados em pré e pós-emergência de um híbrido convencional de milho cultivado no bioma Cerrado do Brasil. Foram instalados dois experimentos de campo, um na safra verão e outro segunda safra. Os tratamentos foram onze herbicidas, cujas doses são apresentadas em g ha-1 de ingrediente ativo: S-metolachlor (1.680), mesotrione + atrazine (115,2 + 2.000), mesotrione + atrazine (192 + 2.000), tembotrione + atrazine (75,6 + 2.000), tembotrione + atrazine (100,8 + 2.000), nicosulfuron + atrazine (16 + 2.000), nicosulfuron + atrazine (24 + 2.000), [mesotrione + atrazine] ([120 + 1.,200]), aplicação sequencial de [mesotrione + atrazine] ([60 + 600]), atrazine (2.000), mais testemunha capinada. Independentemente do experimento, todos os herbicidas aplicados pré e pós-emergência do milho proporcionaram baixos percentuais de fitointoxicação. Em ambos os experimentos nenhum dos tratamentos causou redução no estande, confirmando a ausência de mortalidade das plantas pela aplicação de herbicidas. O tombamento/acamamento das plantas foi observado no experimento realizado de verão e não observou efeito entre tratamentos. Massa de 100 grãos e produtividade do milho não alteraram em função da aplicação de herbicidas na pré e pósemergência da cultura. Todos os tratamentos herbicidas avaliados apresentaram seletividade ao híbrido de milho convencional.

Resumen Aunque no es muy común, en los últimos 20 años se ha observado un aumento significativo en los reportes de rotura del pectoral mayor, normalmente asociado a la práctica de actividad física en la que se produce una contracción intensa y/o al uso de cargas pesadas ejerciendo resistencia sobre el mismo. el músculo. En este caso clínico tenemos un paciente de 51 años que refiere dolor en el pecho derecho y en el brazo derecho durante 3 días después de intentar arreglar el manillar de su motocicleta. Presentó asimetría de pectorales, pérdida de fuerza en miembro superior derecho, dificultad de movimiento y hematoma. La resonancia magnética demostró rotura completa de la unión miotendinosa del pectoral mayor, con tendinopatía con fisuras de inserción e intrasustancial del infraespinoso y tendinopatía con rotura parcial del tendón subescapular. Un médico ortopédico recomendó seguimiento con el uso de medicación analgésica.

Resumo Apesar de não muito frequente, nos últimos 20 anos, houve um aumento significativo dos relatos sobre rotura do peitoral maior, normalmente associadas à prática de atividade física em que ocorre contração intensa e/ou uso de cargas pesadas exercendo resistência sobre o músculo. Neste relato de caso temos um paciente de 51 anos referindo dor no tórax à direita e no braço direito há 3 dias após tentar consertar o guidão da moto. Apresentava assimetria dos peitorais, perda de força do membro superior direito, dificuldade de movimentação e hematoma. A ressonância magnética demonstrou rotura completa da junção miotendínea do peitoral maior, com tendinopatia com fissuras insercionais e intrasubstanciais infraespinhal e tendinopatia com rotura parcial do tendão subescapular. Foi indicado por médico ortopedista o acompanhamento com o uso de medicação analgésica.

Abstract Although not very common, in the last 20 years, there has been a significant increase in reports of rupture of the pectoralis major, normally associated with the practice of physical activity in which intense contraction occurs and/or the use of heavy loads exerting resistance on the muscle. In this case report we have a 51-year-old patient reporting pain in his right chest and right arm for 3 days after trying to fix his motorcycle's handlebars. He had asymmetry of the pectorals, loss of strength in the right upper limb, difficulty moving and hematoma. Magnetic resonance imaging demonstrated complete rupture of the myotendinous junction of the pectoralis major, with tendinopathy with insertional and intrasubstantial infraspinatus fissures and tendinopathy with partial rupture of the subscapularis tendon. An orthopedic doctor recommended follow-up with the use of analgesic medication.

Abstract Introduction: The frequency of depressive symptoms among healthcare professionals during the COVID-19 pandemic was high. However, little evidence includes support workers, those who do not directly provide healthcare. Objective: To identify the prevalence of depressive symptoms and their levels, as well as the associated factors, in Brazilian healthcare workers during the COVID-19 pandemic. Method: This is a cross-sectional, analytical, and quantitative study, conducted with 108 healthcare workers (from direct care and support workers) from January to May 2021, using non-probabilistic sampling. The Depression, Anxiety, and Stress Scale was applied to identify the symptoms of depression. Data were analyzed using absolute and relative frequencies for variables related to sociodemographic, healthcare service and occupation, self-perceived health, factors associated with the risk of COVID-19, clinical history, and depressive symptoms. Association tests were applied, and prevalence ratios were estimated with a confidence interval of 95%. Results: The prevalence of depressive symptoms was 51.9% (95% CI = 43.1%-63.7%), with the extremely severe level being more frequent (16.7%, 95% CI = 8.8%-22.5%), mainly among workers who do not work directly in care (35.7%), compared to those who assist patients (12.9%). The prevalence of depressive symptoms stands out among workers with fair/poor/very poor self-perceived health (88.0%), diagnosis of mental disorder (81.2%), continuous use of medication (63.0%), and use of psychotropic drugs (69.6%). Conclusion: Depressive symptoms were predominant among healthcare workers during the pandemic, with extremely severe levels occurring more frequently among support workers. There is a need to focus attention on the mental health of healthcare workers according to specificities, mainly occupational, to minimize the occurrence of depressive symptoms.

Resumo Introdução: A frequência de sintomas de depressão entre profissionais da saúde durante a pandemia de COVID-19 foi alta. Contudo, existem poucas evidências que incluem trabalhadores de apoio, aqueles que não desenvolvem diretamente a assistência em saúde. Objetivo: Identificar a prevalência da presença de sintomas de depressão e de seus níveis, bem como, os fatores associados em trabalhadores da saúde brasileiros durante a pandemia de COVID-19. Método: Trata-se de uma pesquisa transversal, analítica e quantitativa, realizada com 108 trabalhadores da saúde de janeiro a maio de 2021, por amostragem não probabilística. Aplicou-se a Depression, Anxiety and Stress Scale para identificar os sintomas de depressão. Os dados foram analisados por meio de frequências absolutas e relativas para variáveis sociodemográficas, relacionadas ao serviço de saúde e ocupação, autopercepção de saúde, fatores relacionados ao risco para COVID-19, histórico clínico e “Sintomas de Depressão”. Foram aplicados testes de associação e estimadas Razões de Prevalência com Intervalo de Confiança de 95%. Resultados: A prevalência de sintomas de depressão foi de 51,9% (IC95% = 43,1%-63,7%), sendo que o nível extremamente severo foi mais frequente (16,7%, IC95% = 8,8%-22,5%), principalmente, entre trabalhadores que não atuam diretamente na assistência (35,7%), comparado aos que assistem os pacientes (12,9%). Destaca-se a prevalência de sintomas de depressão entre os trabalhadores com autopercepção de saúde regular/ruim/muito ruim (88,0%), diagnóstico de transtorno mental (81,2%), uso contínuo de medicamentos (63,0%) e uso de psicotrópicos (69,6%). Conclusão: Os sintomas de depressão foram predominantes entre os trabalhadores da saúde em meio à pandemia, ressaltando-se níveis extremamente severos que ocorreram com maior frequência entre trabalhadores de apoio. Sinaliza-se a necessidade de se direcionar a atenção à saúde mental de trabalhadores da saúde conforme especificidades, principalmente, ocupacionais, a fim de minimizar a ocorrência de sintomas de depressão.

Resumen Introducción: La frecuencia de síntomas de depresión entre profesionales de la salud durante la pandemia de COVID-19 fue alta. Sin embargo, hay poca evidencia que incluya a trabajadores de apoyo, quienes no brindan atención médica directamente. Objetivo: Identificar la prevalencia de la presencia de síntomas de depresión y sus niveles, así como factores asociados, en trabajadores de salud brasileños durante la pandemia de COVID-19. Método: Se trata de una investigación transversal, analítica y cuantitativa, realizada con 108 trabajadores de la salud (de asistencia directa y de apoyo) de enero a mayo de 2021, mediante muestreo no probabilístico. Se aplicó la Escala de Depresión, Ansiedad y Estrés para identificar síntomas de depresión. Los datos fueron analizados mediante frecuencias absolutas y relativas para variables sociodemográficas, relacionadas con el servicio de salud y ocupación, salud autopercibida, factores relacionados con el riesgo de COVID-19, historia clínica y síntomas de depresión. Se aplicaron pruebas de asociación y se estimaron razones de prevalencia con un intervalo de confianza de 95. Resultados: La prevalencia de síntomas depresivos fue del 51.9 % (IC95 % = 43.1 %-63.7 %), siendo más frecuente el nivel extremadamente severo (16.7 %, IC95 % = 8.8 %-22.5 %), principalmente entre los trabajadores que no trabajan directamente en el cuidado (35.7 %), en comparación con los que asisten a pacientes (12.9 %). Destaca la prevalencia de síntomas de depresión entre trabajadores con autopercepción de salud regular/mala/muy mala (88.0 %), diagnóstico de trastorno mental (81.2 %), uso continuo de medicación (63.0 %) y uso de psicofármacos (69.6 %). Conclusión: Los síntomas de depresión fueron predominantes entre trabajadores de salud en medio de la pandemia, destacando niveles extremadamente severos que ocurrieron con mayor frecuencia entre personal de apoyo. Destaca la necesidad de orientar la atención a la salud mental de la población trabajadora del área de la salud según especificidades, principalmente ocupacionales, con el fin de minimizar la aparición de síntomas de depresión.

ABSTRACT Introduction: Sarcopenia is defined as the loss of muscle strength associated with a decre- ase in muscle mass and/or functionality, which can lead to frailty syndrome, dependence, and death in older adults. Objective: To determine the frequency of sarcopenia in adults over 75 years old in the Fa- mily Health Unit of the Santa Ana neighborhood. Materials and Methods: Observational, descriptive, cross-sectional study with non-pro- babilistic convenience sampling. Patients of both sexes aged 75 years and older were included. The SARC-F questionnaire was applied, and anthropometric measurements and handgrip strength were taken. Statistical analysis was performed using Epi Info 7.2 6.0 software. Results: A total of 62 adults participated, 64.52% (n=40) were female. The age range was 75 to 92 years. According to the nutritional diagnosis, 35.48% (n=22) were overweight. Sarcopenia was diagnosed in 4.84% (n=3) of the population, and 50% (n=31) presented probable sarcopenia. Conclusion: The frequency of sarcopenia found in this population was lower than the global rate; on the other hand, the figures for probable sarcopenia were similar to those found in Latin America.

RESUMEN Introducción: La sarcopenia se define como la pérdida de fuerza muscular asociada a disminución de la masa y/o de la funcionalidad muscular que puede provocar en el adulto mayor el síndrome de fragilidad, dependencia y óbito. Objetivo: determinar la frecuencia de la sarcopenia en los adultos mayores de 75 años, de la Unidad de Salud de la Familia del Barrio Santa Ana. Materiales y métodos: Estudio observacional, descriptivo, de corte transversal con muestreo no probabilístico por conveniência. Se incluyeron pacientes de ambos sexos a partir de 75 años de edad en los cuales se aplicó el cuestionario SARC-F y se tomó medidas antropométricas y de la fuerza de prensión manual. Para el análisis estadístico se utilizó el software Epi Info 7.2 6.0. Resultados: participaron 62 adultos, el 64,52% (n=40) fue del sexo femenino. El rango de edad fue de 75 a 92 años. Según el diagnóstico nutricional el 35,48% (n=22) presentó sobrepeso. El 4,84% (n=3) de la población fue diagnosticada con sarcopenia y el 50% (n=31) presentó probable sarcopenia. Conclusión: la frecuencia de sarcopenia encontrada en esta población fue menor que la global, por otro lado, las cifras de probable sarcopenia se asemejan a las encontrados en Latinoamérica.

Abstract Cracking can be investigated by infrared thermography, as defects on a surface have different temperatures compared to regions without defects. The temperature of the crack depends on its dimensions, opening and depth, which can be identified by thermography. The possibility of classifying crack damage according to different depths by thermography in heating and cooling regimes and indicating which conditions are most suitable for identifying cracks is evaluated. It is proposed to observe the temperature evolution during the heating and cooling of a mortar slab that has cracks with different depths. The temperature variation of the plate during heating and cooling is monitored by thermograms and thermocouples present on the surface and interior of the plate in regions close to the cracks. It is identified, through the application of Delta-T thermographic contrast, that cracks of greater depth have a greater Delta-T, in modulus, during heating, while cracks of shallower depth have a greater Delta-T during cooling. Cracks are best identified during the cooling phase, as shallower cracks are not identified at the end of heating.

Resumo A fissuração pode ser investigada por termografia de infravermelho, pois defeitos em uma superfície apresentam temperaturas diferentes em relação as regiões sem defeito. A temperatura da fissura é dependente das suas dimensões, abertura e profundidade, nas quais podem ser identificadas pela termografia. Avalia-se o comportamento das temperaturas em regime controlado de aquecimento e resfriamento de fissuras em função de diferentes profundidades e quais as condições mais adequadas para identificar as fissuras. Propõe-se a observação da evolução de temperatura no aquecimento e resfriamento de uma placa de argamassa que possui fissuras com diferentes profundidades. A variação de temperatura da placa no aquecimento e resfriamento é monitorada por termogramas e termopares presentes na superfície e interior da placa em regiões próximas às fissuras. Identifica-se, pela aplicação de contraste termográfico Delta-T, que fissuras de maior profundidade possuem maior Delta-T, em módulo, durante o aquecimento, enquanto fissuras de menor profundidade possuem maior Delta-T no resfriamento. As fissuras são melhor identificadas na fase de resfriamento, pois no fim do aquecimento as fissuras de menor profundidade não são identificadas.

ABSTRACT: The crown lengthening is indicated for aesthetic purposes, exposure of subgingival caries, crown fractures or a combination of these, which can be characterized as aesthetic or functional, related to restorative indications, and restoration of biological width. Several factors need to be evaluated in the aesthetic planning for optimizing the smile, with the inclusion of an increase in the clinical crown, emphasizing the quality of the thin or thick periodontal phenotype, an adequate band of keratinized tissue and the size of the biological width. A correct diagnosis of the gummy smile etiology, as well as an assessment of clinical characteristics and anatomical dimensions, is of fundamental importance prior to the patient's approach. It determines and guide decisions regarding the possibilities of treatment and prognosis of cases. The aim of this study was to report two cases of cosmetic periodontal surgery with techniques commonly used for this purpose: gingivectomy and flap surgery with osteotomy.

RESUMEN: El alargamiento clínico de corona está indicado con fines estéticos, exposición de caries subgingivales, fracturas coronarias o alguna combinación de estas, y puede caracterizarse como estético o funcional, relacionado con indicaciones restaurativas y restauración del espacio biológico. Varios factores deben ser evaluados en la planificación estética para optimizar la sonrisa, con la inclusión del aumento clínico de coronas, destacando la calidad del fenotipo periodontal delgado o grueso, la gama adecuada de tejido queratinizado y la dimensión del espacio biológico. Un diagnóstico correcto de las causas asociadas a la sonrisa gingival, así como una valoración de las características clínicas y dimensiones anatómicas, son de fundamental importancia antes de abordar al paciente, para determinar y orientar decisiones respecto a las posibilidades de tratamiento y pronóstico de los casos. El objetivo de este estudio fue reportar dos casos de cirugía periodontal estética con técnicas comúnmente utilizadas para este fin: gingivectomía y cirugía de colgajo con osteotomía.

Abstract The Cerrado harbors rich biodiversity and yet faces various anthropogenic pressures, an alarming situation that justifies fauna inventory studies, particularly insects, often overlooked in such endeavors. Additionally, many locations within the Cerrado lack information about the odonatofauna, including Conservation Units (CUs), considered the main tools for biodiversity conservation in Brazil. The objective of our study is to report the Odonata community occurring in the Minas Gerais portion of the Parque Nacional Grande Sertão Veredas (PNGSV), the conservation status of species, taxonomic information for some taxa, and compare the Odonata community along wet and dry seasons. The study was conducted from September 2022 to April 2023, with a total of 576 hours of sampling. We collected 601 specimens from 83 species, highlighting two new species (one recently described and other to be described elsewhere), eight new records for the state, and the rediscovery of the species Progomphus geijskesi Needham, 1944, collected for the first time in Brazil since 1918, and Argia botacudo Calvert, 1909, previously known only from the type material. The significant richness sampled, new taxa, new records, and the presence of species at some risk of extinction reinforce the Cerrado's relevance as a global hotspot. It also underscores the importance of CUs as a tool to ensure the protection of Odonata communities in Brazil and highlights the need to strengthen public policies to guarantee investments and foster future financial resources for the protection of Brazilian biota, especially for conducting more inventory studies in the Cerrado.

Resumen El Cerrado alberga una rica biodiversidad y aún enfrenta diversas presiones antropogénicas, una situación alarmante que justifica estudios de inventario de fauna, especialmente de insectos, a menudo pasados por alto en tales esfuerzos. Además, muchos lugares dentro del Cerrado carecen de información sobre la odonatofauna, incluídas las Unidades de Conservación (UC), consideradas las principales herramientas para la conservación de la biodiversidad en Brasil. El objetivo de nuestro estudio es informar sobre la comunidad de odonatos que se encuentra en la porción de Minas Gerais del Parque Nacional Grande Sertão Veredas (PNGSV), el estado de conservación de las especies, información taxonómica para algunos taxones y comparar la comunidad de odonatos durante las estaciones húmeda y seca. El estudio se realizó de septiembre de 2022 a abril de 2023, con un total de 576 horas de muestreo. Recopilamos 601 ejemplares de 83 especies, destacando dos nuevas especies (una recientemente descrita y otra por describirse en otro lugar), ocho nuevos registros para el estado y el redescubrimiento de las especies Progomphus geijskesi Needham, 1944, recolectada por primera vez en Brasil desde 1918, y Argia botacudo Calvert, 1909, conocida anteriormente solo por el material tipo. La riqueza significativa muestreada, los nuevos taxones, los nuevos registros y la presencia de especies en riesgo de extinción refuerzan la relevancia del Cerrado como un hotspot global. También subraya la importancia de las UC como herramienta para garantizar la protección de las comunidades de odonatos en Brasil y destaca la necesidad de fortalecer las políticas públicas para garantizar inversiones y fomentar futuros recursos financieros para la protección de la biota brasileña, especialmente para llevar a cabo más estudios de inventario en el Cerrado.

Abstract From the diversity of phytophagous stink bugs found in rice fields in Itapecuru-Mirim, Maranhão, we have the first record of Edessa meditabunda in the crop, and the second record in Brazil of Paratibraca dubia. Additionally, Tibraca limbativentris, Oebalus poecilus, and Oebalus ypsilongriseus were observed.

Resumen Dentro de la diversidad de chinches fitófagos encontrados en campos de arroz en Itapecuru-Mirim, Maranhão, tenemos el primer registro de Edessa meditabunda en el cultivo y el segundo registro en Brasil de Paratibraca dubia. Además, se observaron Tibraca limbativentris, Oebalus poecilus y Oebalus ypsilongriseus.

SUMMARY: Establishing vegetative propagation techniques to promote the rejuvenation/reinvigoration of genotypes is essential for the rescue of adult trees of the Eucalyptus genus used in species and provenance tests. The aim of this study was to evaluate the induction of epicormic buds and shoots in pruned branches and the in vitro establishment, multiplication, genetic fidelity, and elongation of three 44-year-old Eucalyptus pilularis selected plants. M1, M2 and M3 represent selected plants. M1 produced the highest number of epicormic buds, number of shoots, and with the lowest rate of tissue oxidation and non-reactive explants. In the establishment stage, M3 exhibited the lowest contamination percentage, number of shoots, and shoot length. The emission of shoots occurred only for M3, and it was the unique genotype subjected to the other stages (i.e. multiplication, elongation, and rooting). In the multiplication stage, the highest vigour and shoot length values of were found in the 15th subculture. Phenolic oxidation had its highest value in the 12th subculture, decreasing from the 13th. The highest value for number of shoots was found in the 11th subculture. No polymorphism was observed in the selected plant (M3) and the clonal plants obtained in the 15th subculture. For shoots elongation, the use of culture medium containing 0.10 mg L-1 BAP and 1.00 mg L-1 NAA provided the lowest means for oxidation, and the highest for vigour, number, and length of shoots. The emission of adventitious roots was observed, demonstrating that, through micropropagation, it was possible to induce the competence to root the material, even at an advanced ontogenetic age.

RESUMEN: Establecer técnicas de propagación que promuevan el rejuvenecimiento/revigorización de genotipos es fundamental para el rescate de árboles adultos del género Eucalyptus. El objetivo de este estudio fue evaluar la inducción de yemas y brotes epicórmicos en ramas podadas y el establecimiento, multiplicación, elongación y fidelidad genética de tres plantas seleccionadas de Eucalyptus pilularis de 44 años de edad. M1, M2 y M3 representan las plantas seleccionadas. M1 fue el mejor en número de yemas epicórmicas, número de brotes, baja tasa de oxidación del tejido y explantes que no responden. En la fase de establecimiento, M3 mostró baja contaminación, número de brotes y longitud de brotes. La emisión de brotes ocurrió para M3, y fue el único genotipo sometido a las demás fases (multiplicación, elongación y enraizamiento). En la fase de multiplicación, los mayores valores de vigor y longitud de brotes se encontraron en el subcultivo 15. La oxidación fenólica tuvo su mayor valor en el subcultivo 12, disminuyendo a partir del 13. El valor más alto para el número de brotes se encontró en el subcultivo 11. No se observó polimorfismo entre la planta seleccionada M3 y los brotes obtenidos en el subcultivo 15. En elongación, el uso de medio de cultivo que contenía 0,10 mg L-1 de BAP y 1,00 mg L-1 de ANA proporcionó las medias más bajas para la oxidación, y los más altos para vigor, número y longitud de brotes por explante. Se observó la emisión de raíces adventicias, demostrando que, a través de la micropropagación, era posible inducir la competencia para enraizar el material, incluso en una edad ontogenética avanzada.

ABSTRACT This article aims to analyze, from a Bakhtinian perspective, the grotesque construction of Mexican artist Frida Kahlo’s body through a religious worldview. Therefore, we analyze two of her self-portraits, taking into account the dialogic relations constructed between the grotesque representations and Catholic symbology. The analyzed utterances, in which the specificities of the creative competence of the author-creator in her aesthetic activity are inferred, showed that a dialogue is established between the painting El venado herido and Catholic Saint Sebastian and between the painting Las dos fridas and the image of the Sacred Heart of Jesus.

RESUMO O presente artigo tem como objetivo analisar, sob a perspectiva bakhtiniana, a construção grotesca do corpo fridiano por meio de uma cosmovisão religiosa. Para tanto, são analisados dois autorretratos produzidos pela pintora mexicana Frida Kahlo, levando em consideração as relações dialógicas construídas entre as representações grotescas e as simbologias católicas. Nos enunciados analisados, nos quais são depreendidas as especificidades da competência criativa da autora-criadora em sua atividade estética, percebe-se o correlacionamento entre a pintura El venado herido e a narrativa do santo católico São Sebastião e o diálogo entre a tela Las dos fridas e a imagem do Sagrado Coração de Jesus.