Results: 8
#1
au:Souza, Marcus V. N. de
Filters
Order by
Page
of 1
Next
1.
Cytotoxicity and Leishmanicidal Activity of Isoniazid-Derived Hydrazones and 2-Pyrazineformamide Thiosemicarbazones
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
Amim, Raquel S.
; Firmino, Gisele S. S.
; Rego, Ana C. P. D.
; Nery, Adriane L.
; Da-Silva, Silvia A. G.
; Souza, Marcus V. N. de
; Pessoa, Claudia
; Resende, Jackson A. L. C.
; Figueroa-Villar, José D.
; Lessa, Josane A.
.










The isoniazid-derived hydrazones 2-pyridinecarbaldehyde- (HPCIH), 2-acetylpyridine- (HAPIH), 2-benzoylpyridine- (HBPIH), 2-pyridineformamide- (HPAmIH) and 2-pyrazineformamide- (HPzAmIH) isonicotinoyl hydrazones, as well as the pyrazinamide-derived thiosemicarbazones 2-pyrazineformamide- (HPzAm4DH), N(4)-methyl-2-pyrazineformamide- (HPzAm4M), N(4)-ethyl-2-pyrazineformamide- (HPzAm4E) and N(4)-phenyl-2-pyrazineformamide- (HPzAm4Ph) thiosemicarbazones, were assayed for their action against intracellular amastigote form of Leishmania (Viannia) braziliensis strains and the glioblastoma multiforme (SF-295), colon adenocarcinoma (HCT-116), ovarian cancer (OVCAR-8) and acute myeloid leukemia (HL-60) human tumor cell lines. All compounds exhibited leishmanicidal effects, with concentrations at which 50% of parasites were inhibited (IC50) in the 10.70-18.84 µM range. Moreover, the compounds were up to 30-fold less toxic to macrophages than to the parasites. Pyrazinamide-derived thiosemicarbazones proved to have poor activity against the tumor cell lines at 5 µg mL-1, whereas, in general the isoniazid-derived hydrazones presented good activity, being HAPIH and HBPIH the most potent compounds (IC50 = 0.42-1.5 µM).
https://doi.org/10.5935/0103-5053.20150330
1249 downloads
2.
Optimization of an Electrolyte System for the Simultaneous Separation of Nelfinavir Mesylate and Two Impurities by Micellar Electrokinetic Chromatography
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
A methodology for the simultaneous determination of nelfinavir mesylate and the impurities 3-hydroxy-2-methylbenzoic acid and (2R,3R)-4-((3S,4aS,8aS)-3-(tert-butylcarbamoyl) octahydroisoquinolin-2(1H)-yl)-3-hydroxy-1-(phenylthio)butan-2-aminium benzoate by micellar electrokinetic chromatography, with an analysis time of 25 min, was proposed. An electrolyte composed of sodium tetraborate buffer (pH 9.24; 25 mmol L-1), sodium dodecyl sulphate (9 mmol L-1) and methanol (10%, v/v) was optimized using a mixed-level factorial design, with direct detection at 200 nm. After evaluating some figures of merit, such as selectivity, linearity, precision, limit of detection, limit of quantification, accuracy and robustness (using Youden's test), the method was successfully applied to the analysis of nelfinavir mesylate and its impurities in a pharmaceutical formulation. The optimized methodology is demonstrated to be useful in the determination of these analytes in a synthesis monitoring process, in raw materials and in pharmaceutical formulations, while offering low solvent consumption, requiring a small sample and using non-specific columns as advantages.
https://doi.org/10.5935/0103-5053.20150049
928 downloads
3.
Growing knowledge: an overview of Seed Plant diversity in Brazil
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.





















































































































































































































































































































































































































































Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
https://doi.org/10.1590/2175-7860201566411
33340 downloads
4.
Determination of efavirenz in diluted alkaline electrolyte by cathodic adsorptive stripping voltammetry at the mercury film electrode
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
Journal of the Brazilian Chemical Society
- Journal Metrics
A stripping method for the determination of the antiretroviral drug efavirenz at the submicromolar concentration levels in diluted alkaline electrolyte is described. Optimum experimental conditions were: 2.0 × 10-3 mol L-1 NaOH, accumulation potential of -0.10 V, pulse amplitude of 50 mV and scan rate of 50 mV s-1. The response is linear over the concentration range of 0.01-0.25 ppm. For an accumulation time of 10 min, the limit of detection was 1.0 ppb (3.0 × 10-9 mol L-1). The most convenient conditions to measure the efavirenz concentration in the presence of ATP, DNA, several metals, and other antiviral drugs was also investigated. The utility of the method is demonstrated by the determination of efavirenz in a synthetic mixture containing both lamivudine and zidovudine, which are frequently used in the clinic in association with efavirenz as part of highly active antiretroviral therapy (HAART).
Um método de redissolução para a determinação do agente anti-retroviral efavirenz em concentrações submicromolares e meio eletrolítico alcalino diluído é descrito. As condições experimentais ótimas encontradas são: NaOH 2,0 × 10-3 mol L-1, potencial de acumulação -0,10 V, amplitude de pulso 50 mV e velocidade de varredura 50 mV s-1. A resposta é linear sobre a faixa de concentrações 0,01-0,25 ppm. Para um tempo de acumulação de 10 min, o limite de detecção encontrado foi igual a 1,0 ppb (3,0 × 10-9 mol L-1). As condições mais convenientes para a medida da concentração de efavirenz na presença de ATP, DNA, diversos metais e outros antivirais foi também investigada. A utilidade do método é demonstrada pela determinação de efavirenz em uma mistura sintética contendo lamivudina e zidovudina, drogas frequentemente usadas na clínica em associação com o efavirenz como parte da terapêutica anti-retroviral de alta ação (HAART).
https://doi.org/10.1590/S0103-50532011000900007
1855 downloads
5.
Ethambutol analysis by copper complexation in pharmaceutical formulations: spectrophotometry and crystal structure
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
Faria, Adriana F
; Marcellos, Luiza F
; Vasconcelos, Juliana P
; Souza, Marcus V. N. de
; S. Júnior, Antônio L
; Carmo, Weberton R. do
; Diniz, Renata
; Oliveira, Marcone A. L. de
.








An alternative method for ethambutol (ETB) determination by UV spectrophotometry in pharmaceutical formulations using ETB complexation with Cu(II) was optimized employing a 3² full experimental design having Cu(II) and aqueous acetic acid/sodium acetate buffer at pH 4.6 as variables. The predominant complex stoichiometry in aqueous buffer solution at this pH and formation constant were obtained by using Job's method and Scatchard diagram, respectively. The complex crystalline structure of monocrystal was obtained by X-ray diffraction. The developed method was applied to the pharmaceutical formulation analysis being found: 408.0 ± 11.9 mg of ETB·2HCl, recovery range of 100.9-104.0% and detection and quantification limits of 0.8 and 2.8 mg L-1, respectively. The spectrophotometric method was compared to zone capillary electrophoresis and no significant difference was found within the 95% confidence interval
Um método alternativo para determinação de etambutol (ETB) por espectrofotometria UV em formulações farmacêuticas por complexação com Cu(II) foi otimizado usando um planejamento fatorial completo 3², tendo como variáveis Cu(II) e tampão aquoso de acetato de sódio em pH 4,6. A estequiometria predominante do complexo em solução aquosa tamponada nesse pH e a constante de formação do complexo foram obtidas usando o método de Job e o diagrama de Scatchard, respectivamente. A estrutura cristalina do complexo de monocristal foi obtida por difração de raios X. O método espectrofotométrico foi aplicado na análise de formulação farmacêutica sendo encontrado: 408,0 ± 11,9 mg de ETB·2HCl, faixa de recuperação de 100,9-104,0% e limites de detecção e quantificação de 0,8 e 2,8 mg L-1, respectivamente. O método espectrofotométrico ao ser comparado com o método por eletroforese capilar de zona não evidenciou diferenças significativas no intervalo de 95% de confiança
https://doi.org/10.1590/S0103-50532011000500008
4890 downloads
6.
Synthesis and antitubercular activity of isoniazid condensed with carbohydrate derivatives
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
Cardoso, Sílvia H.
; Assis, João Vítor de
; Almeida, Mauro V. de
; Lourenço, Maria Cristina S.
; Vicente, Felipe R. C.
; Souza, Marcus V. N. de
.






A series of 13 compounds analogous of isoniazid condensed with carbohydrate was synthesized and evaluated for their in vitro antibacterial activity against Mycobacterium tuberculosis H37Rv using Alamar Blue susceptibility test and the activity expressed as the minimum inhibitory concentration (MIC90) in μg/mL. Several compounds exhibited antitubercular activity (0.31-3.12 μg/mL) when compared with first line drugs such as isoniazid (INH) and rifampicin (RIP) and could be a good starting point to develop new compounds against tuberculosis.
https://doi.org/10.1590/S0100-40422009000600038
8578 downloads
7.
Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
An alternative capillary zone electrophoresis (CZE) method for the determination of ciprofloxacin (CPFLX), gatifloxacin (GTFLX), moxifloxacin (MFLX) and ofloxacin (OFLX) through a simple aqueous electrolyte system consisting of 25 mmol L-1 of TRIS/ hydrochloride and 15 mmol L-1 of sodium tetraborate buffer mixture (pH 8.87) using direct UV detection at 282 nm within 3 min was validated. The analytical parameters of validation evaluated were: linearity (r > 0.998), selectivity (comparison between slope of the calibration curve of external standard and calibration curve of standard addition), repeatability in area for sample (RSD%: < 3.94% for CPFLX, < 3.87% for GTFLX, 1.30% for MFLX and < 1.88% for OFLX), intermediate precision in area for sample (RSD%: < 3.59% for CPFLX, < 3.09% for GTFLX, 2.67% for MFLX and < 2.25% for OFLX), accuracy (mean of recovery range: 101.2% for CPFLX, 101.0% for GTFLX, 101.3% for MFLX and 99.9% for OFLX), limit of detection (mg L-1: 2.72 for CPFLX, 1.92 for GTFLX, 0.795 for MFLX and 1.05 for OFLX), limit of quantification (mg L-1: 9.06 for CPFLX, 6.40 for GTFLX, 2.65 for MFLX and 3.50 for OFLX) and robustness. Due to its simplicity, selectivity, precision, accuracy and rapidity, the methodology can be an interesting alternative for quality assurance in the pharmaceutical industry of these drugs.
Um método alternativo por eletroforese capilar de zona (CZE) para determinação de ciprofloxacina (CPFLX), gatifloxacina (GTFLX), moxifloxacina (MFLX) e ofloxacina (OFLX) foi validado. O sistema de eletrólito utilizado consistiu da mistura de 25 mmol L-1 de TRIS/ HCl e 15 mmol L-1 de tetraborato de sódio em meio aquoso resultando em pH 8,87. A análise foi realizada sob detecção direta por UV em 282 nm com tempo de análise de 3 min. Os parâmetros analíticos de validação avaliados foram: linearidade (r > 0,998), seletividade (comparação entre a inclinação da curva de calibração de padronização externa e curva de calibração de adição de padrão), repetitividade em área para amostras (RSD %: < 3,94% para CPFLX, < 3,87% para GTFLX, 1,30% para MFLX e < 1,88% para OFLX), precisão intermediária em área para amostras (RSD%: < 3,59% para CPFLX, < 3,09% para GTFLX, 2,67% para MFLX e < 2,25% para OFLX), exatidão (média da faixa de recuperação: 101,2% para CPFLX, 101,0% para GTFLX, 101,3% para MFLX e 99,9% para OFLX), limite de detecção (mg L-1: 2,72 para CPFLX, 1,92 para GTFLX, 0,795 para MFLX e 1,05 para OFLX), limite de quantificação (mg L-1: 9,06 para CPFLX, 6,40 para GTFLX, 2,65 para MFLX e 3,50 para OFLX) e robustez. Devido à simplicidade, seletividade, precisão, exatidão e rapidez, o método pode ser uma alternativa interessante para auxiliar o controle da qualidade dessas drogas na indústria farmacêutica.
https://doi.org/10.1590/S0103-50532008000300004
3314 downloads
8.
Reações de ortometalacão em piridinas
Facebook Twitter

Facebook Twitter
- Other social networks
- Google+
- StambleUpon
- CiteULike
- Mendeley
- Other networks
- Metrics
In this paper we describe a powerful methodology for the regiospecific construction of polysubstituted aromatic and heteroaromatic compounds. The DoM reaction (direct ortho-metalation) comprises the deprotonation in position ortho of a aromatic or heteroaromatic containing DMG (directed metalation group) by strong bases, normally an alkyllithium reagent, leading to an ortho-lithiated species. These species, upon treatment with electrophilic reagents, gives 1,2 disubstituted products.
https://doi.org/10.1590/S0100-40422002000100016
5307 downloads
Showing
itens per page
Page
of 1
Next
Statistics of
Send result
Sem resultados
No documents were found for your search
Glossary and search help
You can enrich your search in a very simple way. Use the search indexes combined with the connectors (AND or OR) and specify more your search.
For example, if you want to search for articles about
cases of dengue in Brasil in 2015, use:ti:dengue and publication_year:2015 and aff_country:Brasil
See below the complete list of search indexes that can be used:
Index code | Element |
---|---|
ti | article title |
au | author |
kw | article keywords |
subject | subject (title words, abstract and keywords) |
ab | abstract |
ta | journal short title (e.g. Cad. Saúde Pública) |
journal_title | journal full title (e.g. Cadernos de Saúde Pública) |
la | publication language code (e.g. pt - Portuguese, es - Spanish) |
type | document type |
pid | publication identifier |
publication_year | publication year of publication |
sponsor | sponsor |
aff_country | country code of the author's affiliation |
aff_institution | author affiliation institution |
volume | article volume |
issue | article issue |
elocation | elocation |
doi | DOI number |
issn | journal ISSN |
in | SciELO colection code (e.g. scl - Brasil, col - Colômbia) |
use_license | article usage license code |