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Effect of 2-chloro-N-(4-fluoro-3-nitrophenyl)acetamide in combination with antibacterial drugs against Klebsiella pneumoniae
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CORDEIRO, LAÍSA V.

; SOUZA, HELIVALDO D.S.

; SOUSA, ALESON P.

; ANDRADE JÚNIOR, FRANCISCO P. DE

; FIGUEIREDO, PEDRO T.R. DE

; OLIVEIRA, RAFAEL F. DE

; ATHAYDE FILHO, PETRÔNIO F. DE

; OLIVEIRA-FILHO, ABRAHÃO A.

; LIMA, EDELTRUDES DE O.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 N. 2 elocation e20210141

Abstract: En | Text: En | PDF: En

Abstract Klebsiella pneumoniae is a species of Gram-negative bacteria related to a wide range of infections and high rates of drug resistance. The combined use of antibacterial agents is one of the strategies that has been analyzed in recent years as part of the alternatives in the treatment of drug-resistant infections. Recently, the antibacterial activity of of 2-chloro-N-(4-fluoro-3-nitrophenyl)acetamide has been demonstrated against K. pneumoniae, also indicating that this acetamide did not show significant cytotoxic potential in preliminary tests. Thus, it becomes an interesting substance for future studies that explore its antimicrobial capacity, including investigating its association with antibacterial drugs. Based on this, this research aimed to analyze the effects of the association of 2-chloro-N-(4-fluoro-3-nitrophenyl)acetamide (CFA) with ciprofloxacin, cefepime, ceftazidime, meropenem and imipenem against K. pneumoniae strains. The results showed additivity when the substance was combined with ciprofloxacin and cefepime, indifference when associated with ceftazidime and synergistic effect when combined with meropenem and imipenem. Thus, the acetamide was able to optimize the effects of antibacterial drugs, reducing the concentrations necessary to cause bacterial death. These data indicate a potential future clinical use of these combinations, and further studies are needed to analyze this viability.

https://doi.org/10.1590/0001-3765202320210141

Barriers to patient recruitment in a poststroke neurorehabilitation multicenter trial in Brazil
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Silva, T.R. da

; Luvizutto, G.J.

; Martins, L.G.

; Costa, R.D.M. da

; Souza, J.T. de

; Winckler, F.C.

; Sartor, L.C.A.

; Modolo, G.P.

; Ferreira, N.C.

; Rodrigues, J.C.S.

; Kanda, R.G.

; Fogaroli, M.O.

; Borges, G.F.

; Rizzatti, G.R.S.

; Ribeiro, P.W.

; Pires, D.S.

; Favoretto, D.B.

; Aguiar, L.R.

; Bazan, S.G.Z.

; Betting, L.E.G.

; Antunes, L.C.O.

; Nunes, H.R.C.

; Pereira, V.M.

; Edwards, T.G.S.

; Pontes-Neto, O.

; Conforto, A.B.

; Bazan, R.

Brazilian Journal of Medical and Biological Research

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2023, Volume 56 elocation e12326

Abstract: En | Text: En | PDF: En

There is a high demand for stroke rehabilitation in the Brazilian public health system, but most studies that have addressed rehabilitation for unilateral spatial neglect (USN) after stroke have been performed in high-income countries. Therefore, the aim of this study was to analyze USN patient recruitment in a multicenter noninvasive brain stimulation clinical trial performed in Brazil and to provide study design recommendations for future studies. We evaluated the reasons for exclusion of patients from a multicenter, randomized, double-blinded clinical trial of rehabilitation of USN patients after stroke. Clinical and demographic variables were compared between the included and excluded patients. A descriptive statistical analysis was performed. Only 173 of the 1953 potential neglect patients (8.8%) passed the initial screening. After screening evaluation, 87/173 patients (50.3%) were excluded for clinical reasons. Cognitive impairment led to the exclusion of 21/87 patients (24.1%). Low socioeconomic status led to the exclusion of 37/173 patients (21.4%). Difficulty obtaining transportation to access treatment was the most common reason for their exclusion (16/37 patients, 43.3%). The analyzed Brazilian institutions have potential for conducting studies of USN. The recruitment of stroke survivors with USN was restricted by the study design and limited financial support. A history of cognitive impairment, intracranial stenting or craniectomy, and lack of transportation were the most common barriers to participating in a multicenter noninvasive brain stimulation trial among patients with USN after stroke.

https://doi.org/10.1590/1414-431x2023e12326

Glutathione and IGF-1 in bovine seminal cryopreservation: oxidative stress response and pregnancy rate
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Almeida, D.S.

; Pinto, S.C.C.

; Alves, M.B.R.

; Galiza, Y.S.

; Celeghini, E.C.C.

; Laskoski, L.M.

; Osorio, J.P.

; Souza, F.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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mar 2021, Volume 73 N. 2 Pages 311 - 319

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se avaliar a taxa de concepção, as condições metabólicas e estrutural das células espermáticas bovinas criopreservadas, acrescidas de diluidores com fator de crescimento semelhante à insulina do tipo 1(IGF-1) e glutationa (GSH). Foram utilizados 12 ejaculados de touros da raça Nelore, submetidos aos tratamentos: controle, gSH (2mM/mL), IGF-1 (100ng/mL) e gSH (1mM/mL) + IGF-1 (50ng/mL). Após a criopreservação e descongelação, o sêmen passou pelos testes de termorresistência rápida (TTRr), integridade de membrana plasmática e acrossomal (PIAI), alto potencial mitocondrial (AP), estresse oxidativo e taxa de concepção. Utilizou-se o teste de Tukey para as análises estatísticas das variáveis paramétricas e o teste de Friedman para as não paramétricas, com significância de 5%. A percentagem de gestação foi comparada pelo teste do qui-quadrado. Não hove diferença estatística (P<0,05) entre os tratamentos para a variável TTRr. Já no estresse oxidativo avaliado com a sonda CellROX, observou-se que o IGF-1 apresentou maior quantidade de células reativas (P<0,05), 36,38± 24,10. A PIAI, o AP e a taxa de gestação não apresentaram diferença entre tratamentos (P>0,05), com média de concepções de 36,58%. Conclui-se que o IGF-1, a gSH e a sua associação não causaram mudanças na motilidade espermática, no potencial mitocondrial, na integridade da membrana plasmática e acrossomal. O IGF-1 aumentou o estresse oxidativo, porém sem diferença na taxa degestação entre os tratamentos.

ABSTRACT The objective of this study was to evaluate the rate of conception, metabolic, and structural conditions of cryopreserved bovine sperm cells, plus extender with IGF-1 and glutathione (GSH). 12 ejaculations of Nelore bulls were used, submitted to treatments: control, gSH (2mM/mL), IGF-1 (100ng/mL) and gSH (1mM/mL) + IGF-1 (50ng/mL). After cryopreservation and thawing the semen passed the fast thermo resistance test (TTR), plasma membrane and acrosomal integrity (PIAI), mitochondrial membrane potential (AP), oxidative stress, and conception rate. Tukey test was used for the statistical analysis of the parametric variables and the Friedman test for nonparametric. The gestation percentage was compared by the Chi-square test. There was no statistical difference (P<0.05) between treatments for the TTRr variable. Otherwise in the oxidative stress evaluated with the CellROX probe was noted that the IGF-1 showed the highest number of reactive cells (P<0.05). The PIAI, AP and gestation rate showed no difference among treatments (P>0.05), with an average of conceptions of 36.58%. It is concluded that IGF-1, gSH and their association did not cause changes in sperm motility, mitochondrial potential, plasma and acrosomal membrane integrity. IGF-1 increased oxidative stress, however, there was no difference in the gestation rate among the treatments.

https://doi.org/10.1590/1678-4162-12060 233 downloads

Antifungal activity and mechanism of action of 2-chloro-N -phenylacetamide: a new molecule with activity against strains of Aspergillus flavus
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FERREIRA, ELBA S.

; CORDEIRO, LAÍSA V.

; SILVA, DANIELE DE F.

; SOUZA, HELIVALDO D.S.

; ATHAYDE-FILHO, PETRÔNIO F. DE

; BARBOSA-FILHO, JOSÉ MARIA

; SCOTTI, LUCIANA

; LIMA, EDELTRUDES O.

; CASTRO, RICARDO D. DE

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20200997

Abstract: En | Text: En | PDF: En

Abstract Aspergillus genus causes many diseases, and the species Aspergillus flavus is highly virulent. Treatment of aspergillosis involves azole derivatives such as voriconazole and polyenes such as amphotericin B. Due to an increase in fungal resistance, treatments are now less effective; the search for new compounds with promising antifungal activity has gained importance. The aims of this study were to evaluate the effects of the synthetic amide 2-chloro-N-phenylacetamide (A1Cl) against strains of Aspergillus flavus and to elucidate its mechanism of action. Thus, the minimum inhibitory concentration, minimum fungicidal concentration, conidial germination, associations with antifungal agents, cell wall activities, membrane activities and molecular docking were evaluated. A1Cl presented antifungal activity against Aspergillus flavus strains with a minimum inhibitory concentration of between 16 and 256 μg/mL and a minimum fungicidal concentration between 32 and 512 μg/mL. The minimum inhibitory concentration of A1Cl also inhibited conidial germination, but when associated with amphotericin B and voriconazole, it promoted antagonistic effects. Binding to ergosterol on the fungal plasma membrane is the likely mechanism of action, along with possible inhibition of DNA synthesis through the inhibition of thymidylate synthase. It is concluded that the amide 2-chloro-N-phenylacetamide has promising antifungal potential.

https://doi.org/10.1590/0001-3765202120200997 82 downloads

ω-Conotoxina MVIIC e células-tronco mesenquimais promovem recuperação motora em ratos Wistar após trauma medular agudo
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Serra, T.L.

; Santos, F.E.

; Coelho, M.P.R.C.

; Silva, C.M.O.

; Melo, F.G.

; Souza, A.C.S.

; Primo, J.V.C.

; Rodrigues, D.S.

; Gomez, M.V.

; Glória, J.R.

; Ocarino, N.M.

; Serakides, R.

; Melo, E.G.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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dec 2020, Volume 72 N. 6 Pages 2223 - 2232

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste estudo foi avaliar o efeito da ω-conotoxina MVIIC e das células-tronco mesenquimais (CTM) de forma isolada e sua associação nos ratos submetidos ao trauma medular agudo (TMA). Trinta Rattus novergicus, linhagem Wistar, três meses de idade, foram distribuídos igualmente em cinco grupos experimentais: controle negativo (CN), controle positivo (CP), ω-conotoxina MVIIC (MVIIC), células-tronco mesenquimais da medula óssea (CTM-MO) e associação (MVIIC + CTM-MO). O grupo CN foi submetido à laminectomia sem trauma medular, e os grupos CP, MVIIC, CTM-MO e MVIIC + CTM-MO foram submetidos ao trauma medular contusivo. O grupo CP recebeu, uma hora após o TMA, 10μL de PBS estéril, e os grupos MVIIC e MVIIC + CTM-MO receberam 10μL de PBS contendo 20pmol da ω-conotoxina MVIIC, todos por via intratecal. Os grupos CTM-MO e MVIIC + CTM-MO receberam, 24 horas após, 1x106 de CTM via intravenosa. Avaliou-se a recuperação da função locomotora até o sétimo dia pós-trauma. Os animais tratados com MVIIC + CTM-MO obtiveram recuperação motora após o trauma medular agudo (P<0,05). Conclui-se que essa associação apresentou efeito neuroprotetor com melhora na função locomotora em ratos Wistar.

ABSTRACT The objective of this study was to evaluate the effect of isolated ω-conotoxin MVIIC and mesenchymal stem cells (MSCs) and its association in rats submitted to acute spinal cord injury (SCI). Thirty Rattus norvegicus, Wistar strain, three-month-old rats were randomly distributed in five experimental groups with six animals: negative control (CN), positive control (CP), ω-conotoxin MVIIC (MVIIC), bone marrow mesenchymal stem cells (CTM-MO) and the association (MVIIC + CTM-MO). The CN group underwent laminectomy without spinal cord trauma, and groups CP, MVIIC, CTM-MO and MVIIC + CTM-MO were submitted to contusive spinal cord trauma. The CP group received 10μl of PBS one hour after SCI, and groups MVIIC and MVIIC + CTM-MO received 10μl of PBS containing 20pmol of ω-conotoxin MVIIC, both intrathecally. Groups CTM-MO and MVIIC + CTM-MO received 1x106 of MSCs intravenously 24 hours later. The recovery of locomotor function was evaluated up to seven days post-injury. The animals treated with MVIIC + CTM-MO obtained motor recovery after SCI (P<0.05). It is concluded that this association showed neuroprotective effect with improvements in locomotor function in Wistar rats.

https://doi.org/10.1590/1678-4162-11875 604 downloads

Insulina e IGF-1 no meio extensor de criopreservação seminal bovina
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Costa, O.J.S.

; Almeida, D.S.

; Pinto, S.C.C.

; Chaves, R.M.

; Laskoski, L.M.

; Souza, F.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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may 2020, Volume 72 N. 3 Pages 664 - 672

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se avaliar a condição metabólica e estrutural das células espermáticas bovinas após congelação, com adição prévia de IGF-I e insulina no meio diluidor seminal. Os ejaculados de seis touros Nelore foram submetidos a quatro tratamentos: controle; insulina (100µUI/mL); IGF-I (150ng/mL) e insulina + IGF-I (50µUI/mL e 75ng/mL, respectivamente). Após a congelação, realizaram-se os testes de termorresistência rápida, coloração pelo corante azul de tripan e Giemsa, além da análise computadorizada da motilidade espermática, da integridade das membranas plasmática e acrossomal, e da peça intermediária por meio de sondas fluorescentes. O teste de termorresistência rápida apresentou efeito dentro do tempo de cada tratamento, mas não entre os tratamentos. Na análise computadorizada da motilidade espermática, foram observados movimento, motilidade e velocidade espermáticos; não houve efeitos dos tratamentos sobre qualquer uma dessas variáveis. Respostas iguais foram obtidas com as sondas fluorescentes e o corante azul de tripan/Giemsa. A adição de insulina e IGF-I, de forma isolada ou combinada, ao meio diluidor para congelação de sêmen não produziu efeitos sobre as condições metabólica e estrutural das células espermáticas.

ABSTRACT This study aimed to evaluate the metabolic and structural condition of the spermatic bovine cells after the freezing, with addition, previously, of IGF-I and Insulin in the seminal thinner medium. The semen of 6 Nellore bulls were submitted to four treatments: Control, Insulin (100µUI/mL); IGF-I (150ng/mL) and Insulin + IGF-I (50µUI/mL and 75ng/mL, respectively). After freezing, rapid resistance tests, Tripan and Giemsa Blue staining, and computerized analysis of sperm motility and integrity of the plasma and acrosomal membranes and the intermediate part were performed by fluorescent probes. The term rapid resistance test had effect within the time of each treatment, but not between treatments. In the computer analysis of sperm motility, sperm movement, motility and velocity no effects of treatments were observed on any of these variables. The same results were obtained with the fluorescent probes and the Blue dye Trypan / Giemsa. The addition of Insulin and IGF-I, alone or in combination, to the semen freezing dilution medium had no effect on the metabolic and structural condition of sperm cells.

https://doi.org/10.1590/1678-4162-11291 594 downloads

Perfil hematológico e bioquímico sérico de capivaras (Hydrochoerus hydrochaeris) de vida livre nos biomas Mata Atlântica e Caatinga
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Souza, D.S.

; Yang, S.G.N.S.

; Alves, A.C.A.

; Pontes, R.M.

; Carvalho, C.C.D.

; Soares, P.C.

; Oliveira, J.B.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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apr 2020, Volume 72 N. 2 Pages 461 - 470

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Devido à ausência de estudos sobre capivaras na região Nordeste do Brasil, o objetivo deste estudo foi avaliar a sanidade desses roedores de vida livre em três áreas dos biomas Mata Atlântica (2) e Caatinga (1) do estado de Pernambuco, por meio da determinação de parâmetros da hematologia e bioquímica sérica. De novembro de 2016 a dezembro de 2017, foram capturados 21 animais, dos quais foram coletadas amostras de sangue para avaliação hematológica (eritrograma, leucograma e plaquetometria) e bioquímica sérica (atividade enzimática, perfil proteico, energético e mineral). A maioria dos parâmetros esteve dentro dos valores de normalidade para a espécie, embora alguns apresentassem diferenças estatisticamente significativas de acordo com a área de estudo (hemoglobina, hematócrito, VCM, CHCM, eosinófilos, fosfatase alcalina, proteína total, albumina, ácido úrico, creatinina, lactato, sódio e magnésio) e o sexo dos animais (ácido úrico). Os parâmetros obtidos são apresentados como referência e atestam a sanidade e o bom estado nutricional de populações de capivaras nos biomas Mata Atlântica e Caatinga da região Nordeste do Brasil. As informações aportadas neste estudo pioneiro na região Nordeste contribuem para aumentar o conhecimento sobre a ecofisiologia e a conservação in situ de capivaras.

ABSTRACT Due to the lack of studies about capybaras in the northeast region of Brazil, the objective of this study was to evaluate the health status of free-ranging capybaras in three areas of Atlantic Forest (2) and Caatinga (1) biomes in Pernambuco state, through the determination of hematological and serum biochemical parameters. From November 2016 to December 2017, 21 animals were captured and blood samples were collected for the hematological (erythrogram, leukogram and platelet counts) and serum biochemistry (enzymatic activity, protein, energy and mineral profile) evaluation. Hematological and serum biochemical parameters were within the normal range for the species, but some presented statistically significant variations according to the study area (hemoglobin, hematocrit, MCV, MCHC, eosinophils count, alkaline phosphatase, total proteins, albumin, uric acid, creatinine, lactate, sodium and magnesium) and sex of the animals (uric acid). The parameters obtained are presented as reference and attest to the health and good nutritional status of populations of capybaras in the Atlantic Forest and Caatinga biomes of northeastern Brazil. The information provided in this pioneering study in the northeast region contributes to increased knowledge about the ecophysiology and in situ conservation of capybaras.

https://doi.org/10.1590/1678-4162-11163 882 downloads

Does supplementation of vitamin C, reduced glutathione or their association in semen extender reduce oxidative stress in bovine frozen semen?
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Pinto, S.C.C.

; Almeida, D.S.

; Alves, M.B.R.

; Florez-Rodriguez, S.A.

; Abreu Júnior, G.S.

; Alves, N. Britto e

; Celeghini, E.C.C.

; Laskoki, L.M.

; Souza, F.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jan 2020, Volume 72 N. 1 Pages 9 - 17

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste estudo foi avaliar a adição de vitamina C, glutationa reduzida e sua associação ao diluidor de criopreservação de sêmen bovino. O ejaculado de nove touros foi dividido em quatro frações, cada uma correspondendo a um tratamento, a saber: grupo controle - sêmen diluído em Tris-gema; grupo vitamina C - sêmen diluído em Tris-gema, suplementado com vitamina C (2,5mmol/mL); grupo glutationa - sêmen diluído em Tris-gema, suplementado com glutationa reduzida (2,5mmol/mL) e grupo sêmen associado - diluído em Tris-gema, suplementado com vitamina C (1,25mmol/mL) e glutationa reduzida (1,25mmol/mL ). Posteriormente, o sêmen foi envasado em palhetas francesas e submetido à criopreservação por meio de equipamento automatizado. Após a criopreservação, o sêmen foi descongelado e avaliado quanto à motilidade espermática, à morfologia, à membrana plasmática, ao acrossoma, ao potencial mitocondrial e ao estresse oxidativo, bem como pelo teste de resistência térmica. A suplementação de extensor com a associação de vitamina C e glutationa reduzida mostrou efeitos benéficos sobre a motilidade espermática e a preservação das membranas plasmática e acrossomal durante a criopreservação de sêmen, sendo também o grupo que apresentou maiores valores de espécies reativas de oxigênio. Assim, a associação de ambos os antioxidantes contribuiu para a preservação dos espermatozóides em todas as características analisadas, sugerindo sua utilização na criopreservação de sêmen bovino.

ABSTRACT The aim of this study was to evaluate the addition of vitamin C, reduced glutathione and the association thereof to the bovine semen cryopreservation extender. The ejaculate from nine bulls were divided into four fractions, each corresponding to a treatment, namely: control group-semen diluted with Tris-yolk extender; vitamin C group-semen diluted in Tris-yolk extender supplemented with vitamin C (2.5mmol/mL); glutathione group-semen diluted in Tris-yolk extender supplemented with reduced glutathione (2.5mmol/mL) and associated group-semen diluted in Tris-yolk extender supplemented with vitamin C (1.25mmol/mL) and reduced glutathione (1.25mmol/mL). Afterwards, the semen was packed into French straws and submitted to cryopreservation using automated equipment. After cryopreservation, the semen was thawed and evaluated considering sperm motility, morphology, plasma membrane, acrosome, mitochondrial potential and oxidative stress, as well as the thermo resistance test. Extender’s supplementation with the association of vitamin C and reduced glutathione showed benefic effects on sperm motility and preservation of plasma and acrosomal membranes during semen cryopreservation, being also the group that showed higher values of reactive oxygen species. Thus, the association of both antioxidants contributed to the preservation of sperm cells in every analyzed characteristic, suggesting its use on bovine semen cryopreservation.

https://doi.org/10.1590/1678-4162-11293 1115 downloads

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
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Baptista, M.S.

; Alves, M.J.M.

; Arantes, G.M.

; Armelin, H.A.

; Augusto, O.

; Baldini, R.L.

; Basseres, D.S.

; Bechara, E.J.H.

; Bruni-Cardoso, A.

; Chaimovich, H.

; Colepicolo Neto, P.

; Colli, W.

; Cuccovia, I.M.

; Da-Silva, A.M.

; Di Mascio, P.

; Farah, S.C.

; Ferreira, C.

; Forti, F.L.

; Giordano, R.J.

; Gomes, S.L.

; Gueiros Filho, F.J.

; Hoch, N.C.

; Hotta, C.T.

; Labriola, L.

; Lameu, C.

; Machini, M.T.

; Malnic, B.

; Marana, S.R.

; Medeiros, M.H.G.

; Meotti, F.C.

; Miyamoto, S.

; Oliveira, C.C.

; Souza-Pinto, N.C.

; Reis, E.M.

; Ronsein, G.E.

; Salinas, R.K.

; Schechtman, D.

; Schreier, S.

; Setubal, J.C.

; Sogayar, M.C.

; Souza, G.M.

; Terra, W.R.

; Truzzi, D.R.

; Ulrich, H.

; Verjovski-Almeida, S.

; Winck, F.V.

; Zingales, B.

; Kowaltowski, A.J.

Brazilian Journal of Medical and Biological Research

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2019, Volume 52 N. 9 elocation e8935

Abstract: En | Text: En | PDF: En

The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this changing landscape, researchers in biochemistry and molecular biology must re-think their priorities in terms of scientific output dissemination. The evaluation processes and institutional funding for scientific publications should also be revised accordingly. This article presents the results of discussions within the Department of Biochemistry, University of São Paulo, on this subject.

https://doi.org/10.1590/1414-431x20198935 1769 downloads

10.

Estudo anatômico, ultrassonográfico e tomográfico do aparato podotroclear de equinos adultos
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Souza, L.P.

; Bortolini, Z.

; Müller, T.R.

; Fonteque, J.H.

; Schade, J.

; Luciani, M.G.

; Souza, D.S.

; Vulcano, L.C.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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oct 2017, Volume 69 N. 5 Pages 1097 - 1104

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A síndrome do navicular é uma condição que envolve o aparato podotroclear e representa uma das causas mais comuns de claudicação dos membros torácicos de equinos. Portanto, o estudo complementar da região reveste-se de grande interesse quando se refere ao diagnóstico e tratamento das claudicações dos equinos. O objetivo deste estudo foi demonstrar as diferenças dos achados imagenológicos entre a ultrassonografia e a tomografia computadorizada na avaliação das estruturas palmares do aparato podotroclear de equinos adultos e hígidos, bem como a descrição das estruturas observadas nas imagens obtidas com essas técnicas. Para isso, foram realizadas imagens de quatro peças anatômicas, as quais foram posteriormente seccionadas e utilizadas para a descrição anatômica. A utilização de peças anatômicas auxilia no conhecimento da anatomia normal, o que leva à melhor interpretação das imagens e aumenta a especificidade diagnóstica na detecção das alterações que as doenças acarretam. A ultrassonografia fornece informações relevantes quanto às estruturas estudadas, e sua associação com a tomografia computadorizada aumentou a acurácia da investigação. Apesar de o uso da tomografia computadorizada ser mais indicada para tecido ósseo, ela fornece informações importantes, podendo ser usada como uma ferramenta útil quando não se tem disponível a ressonância magnética em razão do custo ou da disponibilidade.

ABSTRACT The navicular syndrome is a condition involving the podotrochlear apparatus and represents one of the most common causes of forelimb lameness in horses. Therefore, further study of this region is of interest when it comes to diagnosis and treatment of lameness in horses. The aim of this study was to demonstrate the differences between the imaging findings of ultrasonography and computed tomography in the evaluation of the palmar structures of the podotrochlear apparatus of healthy adult horses and description of the structures observed in images obtained with these techniques. For this, four images of four anatomical parts were performed, and subsequently sectioned and used for the anatomical description. The use of anatomical parts helps in the understanding of normal anatomy leading to a better interpretation of the images and increasing the specificity of the diagnostic for detecting changes that cause diseases. Ultrasonography provides relevant information about these structures to be studied and the association with computed tomography (CT) increased the accuracy of the investigation. Despite the use of CT being more suitable for bone tissue it provides important information and can be used as a useful tool when there is no available MRI.

https://doi.org/10.1590/1678-4162-9411 6084 downloads

11.

Estenose esofágica em duas cadelas após ovário-histerectomia: relato de caso
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Luciani, M.G.

; Biezus, G.

; Cardoso, H.M.

; Müller, T.R.

; Ferian, P.E.

; Souza, L.P.

; Souza, D.S.

; Casagrande, R.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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aug 2017, Volume 69 N. 4 Pages 908 - 914

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A estenose esofágica benigna é uma afecção rara em pequenos animais, comumente secundária a esofagites ulcerativas. O refluxo gastroesofágico, frequente durante procedimentos anestésicos, é a principal causa de esofagite grave, com consequente formação de cicatriz esofágica. O presente trabalho tem por objetivo descrever dois casos de estenose esofágica ocorrentes após ovário-histerectomia, com destaque para os procedimentos diagnósticos realizados. Em ambos os casos, a combinação dos sinais clínicos sugestivos e os achados de esofagograma e esofagoscopia foram determinantes. No primeiro caso, devido ao tempo avançado de desenvolvimento dos sinais clínicos, o paciente veio a óbito antes mesmo que a intervenção direta da região de estenose fosse realizada. Já no segundo, o procedimento de gastrostomia para melhor manejo alimentar, associado à dilatação esofágica via esofagoscopia e à terapia com medicamentos antiácidos, resultou em melhora clínica.

ABSTRACT Benign esophageal stricture is a rare affection in small animals, usually secondary to ulcerative esophagitis. Gastroesophageal reflux, frequent during anesthetic procedures, is the main cause of severe esophagitis with consequent formation of esophageal cicatrix. The objective of this work is to describe two cases of esophageal stricture occurring after ovariohysterectomy, highlighting the diagnostic procedures performed. In both cases, the combination of the suggestive clinical signs and findings from an esophagram and an esophagoscopy were determinants. In the first case, due to the advanced stage of the clinical signs, the patient died before direct interventions on the stenosis region were performed. But in the second case, the gastrostomy procedures for better feed management associated with esophageal dilatation by esophagoscopy and therapy with antacids resulted in clinical improvement.

https://doi.org/10.1590/1678-4162-9117 1956 downloads

12.

Torção de cólon em um felino doméstico: relato de caso
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Souza, L.P.

; Bach, E.C.

; Lunardeli, B.

; Porto, C.

; Muller, T.R.

; Salbego, F.

; Luciani, M.G.

; Souza, D.S.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2017, Volume 69 N. 3 Pages 597 - 599

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A torção de cólon em felinos é uma patologia rara. Os principais sinais apresentados são de abdome agudo e podem levar o animal rapidamente a óbito. O presente trabalho tem o objetivo de relatar um caso de torção de cólon em um felino. Este é o primeiro relato dessa enfermidade no Brasil. O diagnóstico foi realizado por meio do histórico e de exames de imagem. O animal foi encaminhado para a cirurgia, mas, devido à gravidade e extensão das lesões intestinais, foi realizada a eutanásia.

ABSTRACT Colon torsion in felines is a rare pathology. The main signs presented are of acute abdomen and the condition can quickly lead to death. This paper aims to report a case of twisted colon in a feline. This is the first report of colon torsion in Brazil. The presumptive diagnosis was made through history and imaging. The patient was submitted to surgery, where, due to severity and extent of lesions, euthanasia was performed.

https://doi.org/10.1590/1678-4162-9108 3769 downloads

13.

Efficacy of Topical 5% Acyclovir-1% Hydrocortisone Cream (ME-609) for Treatment of Herpes Labialis: a systematic review
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ROSA, MARIA INÊS DA

; SOUZA, SUÉLI L.

; FARIAS, BRUNA F. DE

; PIRES, PATRÍCIA D.S.

; DONDOSSOLA, EDUARDO R.

; REIS, MARIA EDUARDA F. DOS

Anais da Academia Brasileira de Ciências

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aug 2015, Volume 87 N. 2 Pages 1415 - 1420

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Realizamos uma revisão sistemática com o objetivo de verificar a eficácia do uso tópico de aciclovir 5% e Creme de hidrocortisona 1% em comparação com o grupo placebo para o tratamento herpes labial simplex. Realizamos uma pesquisa bibliográfica usando o Medline, Embase, BIOSIS, LILACS, Scopus, literatura cinza, Central de Registros de Ensaios Controlados Cochrane, o ISI Web of Science e IBECS do início de 1990 a junho de 2014. Nós relatamos os resultados usando risco relativo (RR) com intervalos de confiança de 95%. A pesquisa bibliográfica encontrou 180 publicações potencialmente relevantes. Buscas nas listas de referência encontraram mais duas citações. Entre esses estudos, dois foram considerados elegíveis para inclusão nesta revisão. Ambos os ensaios incluíram 1.213 pacientes. Uma metanálise desses estudos mostrou um RR = 0,77 (95% CI,70-0,86; p <0,001). Este resultado sugere que um tratamento precoce com a combinação de um antiviral e um esteróide é benéfico para o tratamento de herpes labial simplex.

We performed a systematic review with the objective of verifying the efficacy of topical use 5% Acyclovir-1% Hydrocortisone cream compared to the placebo group for herpes simplex labialis treatment. We performed a literature search using MEDLINE, Embase, BIOSIS, LILACS, Scopus, Grey literature, the Cochrane Central Register of Controlled Trials, the ISI Web of Science and IBECS from 1990 to June 2014. We reported the outcomes using relative risk (RR) with 95% confidence intervals. The literature search yielded 180 potentially relevant publications. Reviews of the reference lists yielded two further citations. Among these papers, two were considered eligible for inclusion in this review. Both trials included 1,213 patients. A meta-analysis of these studies showed a RR = 0.77, (95% CI 0.70-0.86; p<0.001).This result suggests that an early episodic treatment with the combination of an antiviral and a steroid is beneficial for herpes simplex labialis treatment.

https://doi.org/10.1590/0001-3765201520140701 4713 downloads

14.

Atividade antimicrobiana do óleo essencial de Lippia origanoides e Lippia rotundifolia frente a enterobactérias isoladas de aves
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Souza, D.S.

; Almeida, A.C.

; Andrade, V.A.

; Marcelo, N.A.

; Azevedo, I.L.

; Martins, E.R.

; Figueiredo, L.S.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2015, Volume 67 N. 3 Pages 940 - 944

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

The aim of this study was to evaluate the antimicrobial activity of essential oils on enterobacteria Escherichia coli and Staphylococcus aureus isolated from poultry fecal samples in the cloaca from 49 laying hens. To analyze the antimicrobial sensibility an agar diffusion susceptibility test was performed and the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericida Concentration (MBC) of Lippia origanoides and Lippia rotundifolia essential oils was determined. The concentrations used were 160, 80 and 40 μL/mL. The L. origanoides essential oil showed antimicrobial effect from 40 μL/mL dose against both microorganisms, with larger efficiency in E. coli. The L.rotundifolia essential oil was more efficient at the concentration of 160μL/mL. Its effect was observed in all microorganisms. These results suggest that L. origanoides oil is more effective than L. rotundifolia oil in inhibiting the growth of microorganism isolated from poultry, although it also has a satisfactory antimicrobial effect. Results indicate the potential use of these plant's essential oils in poultry feed as an alternative to conventional antimicrobial products.

https://doi.org/10.1590/1678-4162-7580 2507 downloads

15.

Use of Doehlert and constrained mixture designs in the development of a photo-oxidation procedure using UV radiation/H2O2 for decomposition of landfill leachate samples and determination of metals by flame atomic absorption spectrometry
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Bezerra, Marcos A.

; Souza, Antônio D.S.

; Oliveira, Rafael V.

; Oliveira, Djalma M.

; Cardoso, Luiz A.M.

; Sousa Filho, Hélio R.

Anais da Academia Brasileira de Ciências

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mar 2015, Volume 87 N. 1 Pages 3 - 13

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este trabalho propõe o uso de degradação por foto-oxidação com radiação ultravioleta e H2O2 como técnica de tratamento de amostras para a determinação de Fe, Zn, Mn, Ni e Co a partir de chorume de aterro sanitário municipal usando espectrometria de absorção atômica com chama (FAAS). Três variáveis (pH, tempo de irradiação e concentração de tampão) foram otimizadas usando planejamento Doehlert e as proporções dos componentes da mistura submetida à irradiação ultravioleta (chorume, solução tampão e H2O2 30% v/v) foram otimizadas usando-se um planejamento de misturas com restrições. Usando as condições experimentais estabelecidas, este procedimento forneceu limites de detecção de 0,075; 0,025; 0,010; 0,075 e 0,041 µg mL-1, e os níveis de precisão, expressos como desvio padrão relativo (%RSD, 0,5 µg mL-1) foram 3,6; 1,8; 1,3; 3,3 e 1,7% pra Fe, Mn, Zn, Ni e Co respectivamente. Testes de recuperação foram realizados para avaliação da exatidão do procedimento e as recuperações encontradas estavam entre 92 e 106% para os metais estudados. Este procedimento foi aplicado nas amostras de chorume coletadas no município de Jequié, região sudoeste da Bahia, Brasil. Os resultados foram comparados com aqueles obtidos pelo procedimento de digestão em meio ácido. Não houve diferenças significativas entre os resultados obtidos nos dois métodos com base no teste t-pareado a um nível de confiança de 95%.

This work proposes the use of photo-oxidation degradation with UV radiation/H2O2 as sample treatment for the determination of Fe, Zn, Mn, Ni and Co in municipal solid waste landfill leachate by flame atomic absorption spectrometry (FAAS). Three variables (pH, irradiation time and buffer concentration) were optimized using Doehlert design and the proportions of mixture components submitted to UV radiation (leachate sample, buffer solution and H2O2 30%, v/v) were optimized using a constrained mixture design. Using the experimental conditions established, this procedure allows limits of detection of 0.075, 0.025, 0.010, 0.075 and 0.041 µg mL-1, and the precision levels expressed as relative standard (%RSD, 0.5 µg mL-1) were 3.6, 1.8, 1.3, 3.3 and 1.7%, for Fe, Mn, Zn, Ni and Co respectively. Recovery tests were carried out for evaluation of the procedure accuracy and recoveries were between 92 and 106% for the studied metals. This procedure has been applied for the analysis of the landfill leachate collected in Jequié, a city of the southwestern region of the State of Bahia, Brazil. The results were compared with those obtained by acid digestion. There was no significant difference between the results obtained by the two methods based on paired t-test at 95% confidence level.

https://doi.org/10.1590/0001-3765201520140258 3194 downloads

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