Abstract The TP53 3’UTR variant rs78378222 A>C has been detected in different tumor types as a somatic alteration that reduces p53 expression through modification of polyadenylation and miRNA regulation. Its prevalence is not yet known in all tumors. Herein, we examine tumor tissue prevalence of rs7837822 in Brazilian cohorts of patients from south and southeast regions diagnosed with lung adenocarcinoma (LUAD, n=586), sarcoma (SARC, n=188) and uterine leiomyoma (ULM, n=41). The minor allele (C) was identified in heterozygosity in 6/586 LUAD tumors (prevalence = 1.02 %) and none of the SARC and ULM samples. Additionally, next generation sequencing analysis revealed that all variant-positive tumors (n=4) with sample availability had additional pathogenic or likely pathogenic somatic variants in the TP53 coding regions. Among them, 3/4 (75 %) had the same pathogenic or likely pathogenic sequence variant (allele frequency <0.05 in tumor DNA) namely c.751A>C (p.Ile251Leu). Our results indicate a low somatic prevalence of rs78378222 in LUAD, ULM and SARC tumors from Brazilian patients, which suggests that no further analysis of this variant in the specific studied regions of Brazil is warranted. However, these findings should not exclude tumor molecular testing of this TP53 3’UTR functional variant for different populations.

Abstract Few long-term studies assess the discoloration induced by hydraulic calcium silicate-based cement on dental structures. In addition, as far as we know, no long-term study has assessed the discoloration induced by these cement on composite resin. Objective This in vitro study aimed to assess, during a period of two years, the discoloration potential of different hydraulic calcium silicate-based cements (hCSCs) on the enamel/dentin structure and composite resin restoration. Methodology A total of 40 enamel/dentin discs were obtained from bovine incisors, and 40 composite resin discs (10 mm in diameter × 2 mm thick) were fabricated. A 0.8 mm-deep cavity was made in the center of each disc and filled with the following hCSCs (n=10): Original MTA (Angelus); MTA Repair HP (Angelus); NeoMTA Plus (Avalon); and Biodentine (Septodont). An initial color measurement was performed (T0 - baseline). After 7, 15, 30, 45, 90, 300 days, and two years, new color measurements were performed to determine the color (ΔE00), lightness (ΔL’), chroma (ΔC’), hue differences (ΔH’), and whiteness index (WID). Results For enamel/dentin, the ΔE00 was significant among groups and periods (p<0.05). NeoMTA Plus had the greatest ΔE00. The NeoMTA Plus group had the greatest ΔE00 after two years for composite resin. Significant reduction in lightness was observed for all groups after two years (p<0.05). The most significant WID values were observed after 30 days for Biodentine (enamel/dentin) and MTA Repair HP groups (composite resin) (p<0.05). Conclusions The hCSCs changed the colorimetric behavior of both substrates, leading to greater darkening over time. The Bi2O3 in the Original MTA seems relevant in the short periods of color change assessment.

Abstract The industries that have polymers as an important raw material in their production, such as the construction, automotive, electrical and electronic sectors, always seek innovations to cut costs, reduce weight, easiness of processing, maximizing mechanical properties, and recyclability. In this context, this work presents the study of the kinetic parameters and viscoelastic behavior of a new thermoplastic system initially liquid. Through differential scanning calorimetry (DSC) analysis, Brookfield viscosimetry; dynamic-mechanical analysis (DMA) and mathematical modeling with consolidated and standardized methods, it was possible to evaluate the polymerization kinetics and viscoelastic behavior of the material in solutions with different concentrations. The generated equations allow the prediction of the kinetic and gelation behavior of the material reducing the need for laboratory tests to determine polymer properties. The found results showed that concentrations of benzoyl peroxide initiator with 1wt% in the methyl methacrylate (MMA) copolymer solution have the best viscoelastic and dynamic-mechanical properties with a less expensive polymerization cycle. production construction automotive sectors costs weight processing recyclability context liquid DSC (DSC viscosimetry dynamicmechanical dynamic DMA (DMA methods 1wt wt MMA (MMA cycle

Resumo O uso prejudicial de substâncias psicoativas tem sido considerado um dos maiores problemas de saúde pública. Diversas abordagens são utilizadas para o tratamento dos transtornos relacionados ao uso de substâncias diante dos desafios impostos. O presente estudo teve por objetivo identificar os trabalhos empíricos que utilizaram a ACT para o tratamento do uso de substâncias e seus respectivos resultados no que diz respeito à aceitação, viabilidade e redução da gravidade da dependência, bem como nos resultados relacionados às comorbidades e demais aspectos associados ao uso através de uma revisão sistemática da literatura acerca do tema, utilizando-se das recomendações do guia Preferred Report Items for Systematic Reviews and Meta-Analyses (PRISMA) e buscas nas bases Pubmed, APA PsycNET, SciELO, ScienceDirect, Web of Science, BVS e LILACS. Foram analisados 17 estudos, permitindo observar aumento da flexibilidade psicológica, atenuação das taxas de abandono do tratamento e redução do consumo de substâncias nas intervenções analisadas.

Abstract The harmful use of psychoactive substances has been considered one of the biggest public health issues. Several approaches are used for the treatment of disorders related to substance use in the face of the challenges imposed. This study aimed to identify the empirical works that used ACT for the treatment of substance use and their respective results regarding acceptance, feasibility, and reduction in the severity of dependence, as well as the results related to comorbidities and other aspects associated with drugs use by a systematic review of the literature on the subject, using the recommendations of the Preferred Report Items for Systematic Reviews and Meta-Analyses (PRISMA) guide and searches in PubMed, APA PsycNET, SciELO, ScienceDirect, Web of Science, VHL, and LILACS. In total, 17 studies were analyzed, allowing the observation of an increase in psychological flexibility, attenuation of treatment dropout rates, and a reduction in substance use in the analyzed interventions.

Resumen El uso nocivo de sustancias psicoactivas se ha considerado como uno de los mayores problemas de salud pública. Se utilizan varios enfoques para el tratamiento de los trastornos relacionados con el consumo de sustancias frente a los desafíos impuestos. Este estudio tuvo como objetivo identificar los trabajos empíricos que utilizaron ACT para el tratamiento del consumo de sustancias y sus respectivos resultados en cuanto a la aceptación, viabilidad y reducción de la gravedad de la dependencia, así como los resultados relacionados con las comorbilidades y otros aspectos asociados al uso, desde una revisión sistemática de la literatura sobre el tema y utilizando las recomendaciones de la guía Preferred Report Items for Systematic Reviews and Meta-Analyzes (PRISMA) y búsquedas en PubMed, APA PsycNET, SciELO, ScienceDirect, Web of Science, VHL y LILACS. Se analizaron 17 estudios, lo que permitió observar un aumento de la flexibilidad psicológica, una atenuación de las tasas de abandono del tratamiento y una reducción del consumo de sustancias en las intervenciones analizadas.

Na Mata Fresca, área situada na divisa dos Estados do Rio Grande do Norte e Ceará, pequenos agricultores têm como fonte de renda a obtida com a produção de raízes de mandioca, usando densidades de plantio em torno de 5.000 plantas ha-1. Esse procedimento pode estar limitando a obtenção de maiores rendimentos de raízes, pois alguns trabalhos demonstraram que é possível a obtenção de rendimentos maiores da mandioca, com maiores densidades. O objetivo do trabalho foi avaliar o rendimento de raízes e outras características da mandioca em resposta à densidade de plantio. A cultivar Vermelhinha foi submetida às densidades de plantio de 5.000 a 21.000 plantas ha-1, com intervalos de 2.000 plantas ha-1, em experimento irrigado. Utilizou-se o delineamento de blocos completos casualizados com quatro repetições. As densidades de plantios ideais para maximizar a obtenção de matérias frescas de folhas, caule + ramos, total de raízes, de raízes comercializáveis, número de raízes comercializáveis, e de matérias secas de raízes comercializáveis e da parte aérea foram de 17.800; 17.077; 14.416; 13.594; 16.436; 12.361 e 18.149 plantas ha-1, respectivamente. Com a adoção da densidade de plantio usada pelos agricultores, obtiveram-se 15.837 kg ha-1 de raízes comercializáveis. Com o uso da densidade ideal encontrada no presente trabalho (13.594 plantas ha-1), o rendimento foi mais do dobro do rendimento do agricultor. O aumento da densidade de plantio reduziu o comprimento das raízes comercializáveis e o índice de colheita (relação entre a matéria seca das raízes comercializáveis e a matéria seca total da planta).

In Mata Fresca, an area located on the border of the states of Rio Grande do Norte and Ceará, in Brazil, small farmers have a source of income from the production of cassava roots, using planting densities of around 5,000 plants ha-1. This procedure might be helping to limit higher yields of the roots, since some studies have shown that it is possible to obtain higher yields of cassava using higher densities. The objective of this study was to evaluate the root yield and other characteristics of the cassava, as a response to planting density. The Vermelhinha cultivar was submitted to planting densities of from 5,000 to 21,000 plants ha-1, at intervals of 2,000 plants ha-1, in an experiment under irrigation. A completely randomized block design with four replications was used. The ideal planting densities in order to maximise leaf green matter, stems and branches, total roots, total marketable roots, number of marketable roots, marketable-root dry matter and stem dry matter, were 17,800; 17,077; 14,416; 13,594; 16,436; 12,361; and 18,149 plants ha-1 respectively. When adopting the planting density used by the farmers, a yield for marketable roots of 15,837 kg ha-1 was obtained. By using the optimal density as found in this work (13,594 plants ha-1), the yield was more than double that of the farmers. Increasing planting density reduced both the length of the marketable roots and the harvest index (the ratio of marketable-root dry matter to total plant dry matter).

O objetivo do estudo foi analisar a influência da cafeína no desempenho dos 200 metros rasos (200 m). Dezessete indivíduos fisicamente ativos (21,5 ± 2,15 anos; 175,9 ± 5,5 cm; 74,1 ± 10,04 kg) executaram em dias diferentes duas performances de 200m. Uma hora antes do teste foi ingerido de modo duplo-cego e randomizado cápsula gelatinosa contendo cafeína (6mg.kg-1) ou placebo. Foram analisados o tempo final dos 200 m rasos e o lactato sanguíneo ([La]; repouso, pré-aquecimento e pós-teste). A ingestão de cafeína diminuiu significantemente o tempo no desempenho dos 200m em relação ao placebo (27,398 ± 1,626 vs. 27,596 ± 1,714 s, respectivamente) e aumentou as [La] pré-aquecimento (1,236 ± 0,497 vs 1,064 ± 0,330 mM) sem modificações na [La] pico. Assim, podemos concluir que a ingestão de cafeína exerceu efeito ergogênico no desempenho com característica anaeróbia, nos indivíduos ativos avaliados neste estudo. Contudo a ausência de modificação na [La] pico indica que essa melhora não parece estar relacionada a um maior fluxo glicolítico.

The aim of this study was to investigate the influence of caffeine ingestion on 200 meters performance (200 m). Seventeen physical education students (21.5 ± 2.15 years; 175.9 ± 5.5 cm; 74.1 ± 10.04 kg) performed on two different days two performances of 200 m. One hour before the performance the participants ingested a gelatin capsule containing either caffeine (6 mg.kg-1) or placebo in a randomized double-blinded manner. Were analyzed the end time of the 200 m race and blood lactate ([La]; rest, pre-warm-up and post-test) Caffeine intake decreased the time to overcome 200 m race compared to placebo intake (27.398 ± 1.626 and 27.596 ± 1.714 s, respectively) and increased blood lactate concentration [La] pre-warm-up (1.236 ± 0.497; 1.064 ± 0.330 mM, respectively) without modification in [La] peak. Thus, we conclude that caffeine intake exert an ergogenic effect on anaerobic performance, however analyzing the [La] peak, this improvement does not seem to be related to increased glycolytic flux.

OBJETIVO: Avaliar se há preferência pela citação de periódicos estrangeiros em detrimento dos brasileiros, em três revistas brasileiras de cirurgia, em dois períodos distintos. MÉTODOS: Foram avaliadas todas as referências dos artigos publicados nos anos de 2011 e 2007, pelas revistas Acta Cirúrgica Brasileira, Arquivos Brasileiros de Cirurgia Digestiva e Revista do Colégio Brasileiro de Cirurgiões, verificando a quantidade de artigos provenientes de revistas brasileiras e estrangeiras. RESULTADOS: Foram analisadas 7343 referências dispostas em 348 artigos nas três revistas analisadas, desse total, 856 (11,65%) eram de periódicos brasileiros. Não houve diferença entre as três revistas analisadas, nem entre os dois períodos analisados. Cento e onze (31,9%) artigos não citaram artigo de periódico brasileiro e 36 (10,34%), citaram mais os de brasileiros do que os de estrangeiros. CONCLUSÃO: há necessidade de o pesquisador brasileiro valorizar mais os periódicos brasileiros aumentando a citação do Brasil, sem uma xenofobia científica.

OBJECTIVE: To evaluate whether there is a preference for foreign periodicals over Brazilian one. METHODS: We evaluated all references of articles published in the years 2011 and 2007 in the journals Acta Cirúrgica Brasileira, Arquivos Brasileiros de Cirurgia Digestiva and the Journal of the Brazilian College of Surgeons, verifying the quantity of papers from Brazilian and foreign publications. RESULTS: We analyzed a total of 7,343 references arranged in 348 articles in those three journals; of these, 856 (11.65%) were from Brazilian journals. There was no difference between the three magazines, nor between the two periods. One hundred and eleven (31.9%) articles did not cite any Brazilian journal article and 36 (10.34%) cited Brazilian articles more than foreign ones. CONCLUSION: There is need for the Brazilian researcher to appreciate more the Brazilian journals, increasing citation from Brazil, without a scientific xenophobia.

Schinus terebinthifolius Raddi é uma espécie florestal nativa que pode ser utilizada na recomposição de áreas degradadas e na arborização urbana. Para avaliar a eficiência de métodos que possibilitem separar lotes de sementes de aroeira-vermelha em níveis de vigor, foram utilizados cinco lotes de sementes avaliados qualitativamente pela determinação do teor de água, germinação, primeira contagem da germinação, envelhecimento acelerado (procedimento tradicional e o método alternativo com o uso de solução saturada de NaCl), índice de velocidade de germinação, tempo médio de germinação, comprimento e massa seca de plântulas. Os testes de primeira contagem da germinação, envelhecimento acelerado a 41 °C por 72 h, tempo médio de germinação, comprimento de plântulas e matéria seca de plântulas permitem a separação dos lotes de sementes de S. terebinthifolius em níveis de vigor.

Schinus terebinthifolius is a native forest species which may be used in recuperating degraded areas and in urban afforestation. In order to evaluate the efficiency of methods for separating lots of S. terebinthifolius seeds into vigor levels, five lots of seeds were qualitatively evaluated for their moisture content, germination, first count, accelerated aging (the traditional and the saturated salt methods), germination speed index, average time of germination and seedling length and dry weight. The results showed that the tests of first germination count, accelerated aging at 41 ºC for 72 hours, average time of germination, seedling length and dry matter permitted the separation of S. terebinthifolius seeds into different vigor levels.

O artigo resgata a essência da relação orientador-orientado como núcleo de sustentação de um programa de pós-graduação senso estrito e suas influências e implicações no processo atual de formação dos pesquisadores.

The article captures the essence of the advisor-advisee relationship as the fulcrum of the graduate program, and its influence and implications in the present formation process of new researchers.