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Abstract Objective Advances in medicine have increased the life expectancy of pediatric patients with chronic illnesses, and challenges with the guided transition of adolescents and young adults from pediatric clinics to adult clinics have grown. The aim of this study was to better understand readiness and factors related to this transition process in Brazil. Method In this cross-sectional study of 308 patients aged from 16 to 21 years under follow-up in pediatric specialties, the degree of readiness for transition was assessed using the Transition Readiness Assessment Questionnaire (TRAQ) and its domains. Associations with demographic data, clinical data, socio-economic level, medication adherence, family functionality, and parental satisfaction with health care were evaluated. Results The median TRAQ score was 3.7 (3.2 - 4.2). Better readiness was associated with female patients, socio-economic class A-B, current active employment, higher level of education, not failing any school year, attending medical appointments alone, functional family, and a good knowledge of disease and medications. A low correlation was observed between TRAQ and age. TRAQ presented good internal consistency (alpha-Cronbach 0.86). In the multiple linear regression, TRAQ score showed a significant association with female gender, advanced age, socio-economic class A-B, better knowledge of disease and medications, and independence to attend appointments alone. Conclusion TRAQ instrument can guide healthcare professionals to identify specific areas of approach, in order to support adolescents with chronic disease to set goals for their own personal development and improve their readiness to enter into the adult healthcare system. In this study, some factors were related to better TRAQ scores.

https://doi.org/10.1016/j.jped.2022.10.006

Challenges in Developmental Psychology Research During the COVID-19 Pandemic
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Frizzo, Giana Bitencourt

; Arteche, Adriane Xavier

; Yates, Denise Balem

; Sousa, Diogo Araújo de

; Mendonça Filho, Euclides de

; Bazon, Marina Rezende

; Silva, Mônia Aparecida da

; Silva, Patrícia Santos da

; Mateus, Vera Lúcia Esteves

; Marasca, Aline Riboli

; Cauduro, Giovanna Nunes

; Almeida, Maíra Lopes

; Bandeira, Denise Ruschel

Psico-USF

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jul 2022, Volume 27 N. 3 Pages 567 - 580

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo A pandemia COVID-19 trouxe uma série de reestruturações necessárias à pesquisa em Psicologia do Desenvolvimento. O objetivo deste artigo é discutir as adaptações que realizamos em pesquisas neste contexto durante a pandemia de COVID-19 e apresentar estratégias para adequação de protocolos de pesquisa originalmente planejados para ocorrer de forma presencial. Embora alguns contextos não permitam a continuidade dos estudos, pesquisas nesse momento podem trazer importantes contribuições sobre este período ímpar. No presente artigo são exploradas estratégias de adaptação dos procedimentos de recrutamento, sugeridas plataformas de divulgação e como melhor usar as redes sociais para esse fim. Também são descritos procedimentos para realização de entrevistas não presenciais com responsáveis, formas de avaliação da interação das duplas mãe-criança e problematizadas questões éticas. Os procedimentos de devolução dos resultados, um compromisso ético dos pesquisadores, podem ser facilitados por recursos como relatórios automáticos. Além disso, sugerimos estratégias para melhor divulgação dos resultados ao público participante.

Abstract The COVID-19 pandemic brought a series of restructurings necessary for research in Developmental Psychology. The aim of the manuscript is to discuss adaptations we made in our research in this context during the COVID-19 pandemic and to present strategies to adequate research protocols originally designed to occur in person. Although some contexts do not allow the continuity of studies, research at this time can bring essential contributions in this extreme period. This article explores the strategies for adapting recruitment procedures, suggesting dissemination platforms, and using social networks for this purpose. Guidelines are suggested for conducting non-face-to-face interviews with caregivers, ways of assessing the interaction of the mother-child pairs, and problematizing ethical issues. The procedures for returning the results, an ethical researcher commitment, may be improved by resources such as automatic reports. Besides, strategies for better dissemination of the results for the participants are suggested.

Resumen La pandemia del COVID-19 trajo una serie de reestructuraciones necesarias para la investigación en Psicología del Desarrollo. El objetivo de este artículo es discutir las adaptaciones realizadas en las investigaciones en este contexto durante la pandemia de COVID-19 y presentar algunas estrategias para la adaptación de los protocolos de investigación originalmente planeados para ser presenciales. Si bien algunos contextos no permitan la continuidad de los estudios, la investigación en este momento puede aportar importantes avances sobre estos tiempos de crisis. Este artículo explora las estrategias para adaptar los procedimientos de contratación, sugiriendo algunas plataformas de difusión y la mejor manera de utilizar las redes sociales para este fin. También se describen los procedimientos para la realización de entrevistas no presenciales con padres o tutores legales, las formas de evaluar la interacción madre-hijo y las cuestiones éticas. Los procedimientos para la devolución de los resultados, un compromiso ético de los investigadores, pueden verse facilitados por funciones como informes automáticos. Además, se recomienda estrategias para una mejor difusión de los resultados al público participante.

https://doi.org/10.1590/1413-82712034270313

Postos de trabalho ocupados por fisioterapeutas: uma menor demanda para a atenção básica
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Fernandes, Juliana Aparecida Elias

; Gomes, Marília Miranda Forte

; Sousa, Bruna da Silva

; Marães, Vera Regina Fernandes da Silva

Ciência & Saúde Coletiva

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jun 2022, Volume 27 N. 6 Pages 2175 - 2186

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Tendo em vista o olhar ampliado de saúde proposto pelo SUS, a abordagem dada ao processo do adoecimento exigiu um prisma voltado à visão biopsicossocial, e não mais ao modelo biomédico, o que levou a um reordenamento na formação dos profissionais de saúde. Assim, as Diretrizes Curriculares Nacionais foram instituídas norteando os projetos pedagógicos dos cursos rumo ao modelo biopsicossocial, de forma que a Classificação Internacional de Funcionalidade, Incapacidade e Saúde contribui para esse cenário por meio de sua abordagem multidimensional e não reducionista. Entretanto, historicamente, a fisioterapia tem forte influência do modelo biomédico, distanciando-a da atenção básica (AB). Este é um estudo exploratório, descritivo e quantitativo, com fonte de dados secundários obtidos por meio do Cadastro Nacional dos Estabelecimentos de Saúde do Brasil, e cujo objetivo foi verificar, dentro de um intervalo de dez anos, em qual nível de assistência à saúde está concentrada a maior parte dos fisioterapeutas e sua representatividade na AB. Os achados indicaram que a AB representa o cenário minoritário dos postos de trabalho ocupados pelos fisioterapeutas, refletindo a hegemonia do modelo biomédico e reforçando o caráter reabilitador dado historicamente à profissão.

Abstract In view of the expanded view of health proposed by the Unified Health System (SUS), the approach given to the illness process required a prism focused on the biopsychosocial view and no longer on the biomedical model, which led to a reorganization in the training of health professionals. Thus, the National Curriculum Guidelines were established guiding the pedagogical projects of the courses towards the biopsychosocial model, so that the International Classification of Functionality, Disability and Health contributes to this scenario through its multidimensional and non-reductionist approach. However, physiotherapy has historically been strongly influenced by the biomedical model, distancing it from primary care (PC). This is an exploratory, descriptive and quantitative study, with secondary data source obtained through the National Register of Health Establishments in Brazil, whose objective was to verify, within a 10-year interval, at which level of health care most physiotherapists and their representation in PC are concentrated. The findings indicated that PC represents the minority scenario of jobs occupied by physical therapists, reflecting the hegemony of the biomedical model and reinforcing the rehabilitation character historically given to the profession.

https://doi.org/10.1590/1413-81232022276.14692021

Postos de trabalho ocupados por fisioterapeutas: uma menor demanda para a atenção básica
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Fernandes, Juliana Aparecida Elias

; Gomes, Marília Miranda Forte

; Sousa, Bruna da Silva

; Marães, Vera Regina Fernandes da Silva

Ciência & Saúde Coletiva

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jun 2022, Volume 27 N. 6 Pages 2175 - 2186

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1413-81232022276.14692021

BRAZILIAN CONSENSUS ON THE MANAGEMENT OF INFLAMMATORY BOWEL DISEASES IN PEDIATRIC PATIENTS: A CONSENSUS OF THE BRAZILIAN ORGANIZATION FOR CROHN’S DISEASE AND COLITIS (GEDIIB)
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LOMAZI, Elizete Aparecida

; OBA, Jane

; RODRIGUES, Maraci

; MARMO, Michela Cynthia da Rocha

; SANDY, Natascha Silva

; SDEPANIAN, Vera Lucia

; IMBRIZI, Marcello

; BAIMA, Júlio Pinheiro

; MAGRO, Daniéla Oliveira

; ALBUQUERQUE, Idblan Carvalho de

; ZABOT, Gilmara Pandolfo

; CASSOL, Ornella Sari

; SAAD-HOSSNE, Rogério

Arquivos de Gastroenterologia

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2022, Volume 59 Suppl. 1 Pages 85 - 124

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Background: Approximately 25% of patients with inflammatory bowel disease (IBD) develop the disease during childhood or adolescence and treatment aims to control active symptoms and prevent long-term complications. The management of Crohn’s disease (CD) and ulcerative colitis (UC) can be especially challenging in children and adolescents, related to particularities that may affect growth, development, and puberty. Objective: This consensus aims to provide guidance on the most effective medical and surgical management of pediatric patients with CD or UC. Methods: Experts in Pediatric IBD representing Brazilian gastroenterologists (Brazilian Organization for Crohn’s Disease and Colitis [GEDIIB]) developed this consensus. A rapid review was performed to support the recommendations/statements. Medical and surgical recommendations were structured and mapped according to the disease type, disease activity, and indications and contraindications for medical and surgical treatment. After structuring the statements, the modified Delphi Panel methodology was used to conduct the voting. The process took place in three rounds: two using a personalized and anonymous online voting platform and one face-to-face. Whenever participants did not agree with a specific recommendation, an option to explain why was offered to enable free-text responses and provide the opportunity for the experts to elaborate or explain disagreement. The consensus of recommendations in each round was accepted when reached ≥80% agreement. Results and conclusion: The recommendations are presented according to the stage of treatment and severity of the disease in three domains: management and treatment (drug and surgical interventions), criteria for evaluating the effectiveness of medical treatment, and follow-up/ patient monitoring after initial treatment, follow-up/ patient monitoring after initial treatment. Surgical recommendations were grouped according to disease type and recommended surgery. The target audience for this consensus was general practitioners, gastroenterologists, and surgeons interested in the treatment and management of pediatric CD and UC. Additionally, the consensus aimed to support the decision-making of health insurance companies, regulatory agencies, and health institutional leaders and/or administrators.

RESUMO Contexto: Aproximadamente 25% dos pacientes desenvolvem doença inflamatória intestinal (DII) durante a infância ou adolescência, e o tratamento visa controlar os sintomas ativos e prevenir complicações a longo prazo. O tratamento da doença de Crohn (DC) e retocolite ulcerativa (RCU) pode ser especialmente desafiador em crianças e adolescentes, relacionado a particularidades que podem afetar o crescimento, o desenvolvimento e a puberdade. Objetivo: Este consenso visa fornecer orientações sobre o tratamento clínico e cirúrgico mais eficaz de pacientes pediátricos com DC ou RCU. Métodos: Gastroenterologistas brasileiros especialistas em DII Pediátrico membro da Organização Brasileira para Doença de Crohn e Colite (GEDIIB) desenvolveram este consenso. Uma revisão rápida foi realizada para apoiar as recomendações/declarações. As recomendações médicas e cirúrgicas foram estruturadas e mapeadas de acordo com o tipo de doença, atividade da doença e indicações e contraindicações para tratamento médico e cirúrgico. Após a estruturação das declarações, foi utilizada a metodologia modificada do Painel Delphi para conduzir a votação. O processo ocorreu em três rodadas: duas por meio de uma plataforma de votação online personalizada e anônima e uma presencial. Sempre que os participantes não concordavam com a recomendação específica, uma opção para explicar o motivo era oferecida para permitir respostas em texto livre e dar a oportunidade para os especialistas elaborarem ou explicarem a discordância. O consenso das recomendações em cada rodada foi aceito quando houve concordância ≥80%. Resultados e conclusão: As recomendações são apresentadas de acordo com o estágio de tratamento e gravidade da doença em três domínios: manejo e tratamento (intervenções medicamentosas e cirúrgicas), critérios para avaliar a eficácia do tratamento médico, acompanhamento/monitoramento do paciente após tratamento. As recomendações cirúrgicas foram agrupadas de acordo com o tipo de doença e cirurgia recomendada. O público-alvo deste consenso foram clínicos gerais, gastroenterologistas e cirurgiões interessados no tratamento e manejo da RCU e DC pediátrica. Além disso, o consenso visava apoiar a tomada de decisão das operadoras de planos de saúde, agências reguladoras e líderes e/ou administradores de instituições de saúde.

https://doi.org/10.1590/s0004-2803.2022005s1-04

Colonization by Panstrongylus megistus (Hemiptera, Reduviidae, Triatominae) in an urban park in the city of São Paulo
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Silva, Rubens Antonio da

; Estevão, Vera Aparecida Oliveira

; Duarte, Agnaldo Nepomuceno

; Maria, Priscilla Cipolini

Revista da Sociedade Brasileira de Medicina Tropical

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2021, Volume 54 elocation e03302020

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: This communication reports on the occurrence of colonization by Panstrongylus megistus in an urban park in the municipality of São Paulo, Brazil. METHODS: Entomological research includes active search for vectors based on notifications by the population and identification and examination of insects. RESULTS: A colony of triatomines was found to be associated with enclosed birds. CONCLUSIONS: The occurrence of P. megistus has already been reported in the city of São Paulo; however, reports of colonization by this species provide evidence of its potential for the occupation of artificial ecotopes, which may pose a risk to the human population.

https://doi.org/10.1590/0037-8682-0330-2020 426 downloads

Colonization of Panstrongylus megistus (Hemiptera:Reduvidae:Triatominae) in an urban area and its association with Didelphis marsupialis in the metropolitan region of São Paulo
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Silva, Rubens Antonio da

; Zaicaner, Raquel

; Rosa, Mariangela Palma

; Aun, Graciela Cristina Granizo

; Muniz, Júlio César

; Magalhães, Alon Carlos

; Pereira, Creusa

; Pena, Amanda Rosa

; Santos, Maysa Flexa dos

; Estevão, Vera Aparecida Oliveira

; Duarte, Agnaldo Nepomuceno

Revista da Sociedade Brasileira de Medicina Tropical

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2021, Volume 54 elocation e0471-2020

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: This communication reports the colonization of Panstrongylus megistus in an urban area of the municipality of Taboão da Serra in the metropolitan region of São Paulo. METHODS: After receiving a notification from the population, entomological research comprising active search, collection, identification, and examination of triatomines was conducted. Wild animals were captured and examined. RESULTS: A colony of triatomines was found to be associated with dogs in the backyard of the property. CONCLUSIONS: The colonization of P. megistus shows the potential for their occupation of artificial ecotopes, which may pose a risk to the human population.

https://doi.org/10.1590/0037-8682-0471-2020 274 downloads

Differences in children and adolescents with SARS-CoV-2 infection: a cohort study in a Brazilian tertiary referral hospital
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Marques, Heloisa Helena de Sousa

; Pereira, Maria Fernanda Badue

; Santos, Angélica Carreira dos

; Fink, Thais Toledo

; Paula, Camila Sanson Yoshino de

; Litvinov, Nadia

; Schvartsman, Claudio

; Delgado, Artur Figueiredo

; Gibelli, Maria Augusta Bento Cicaroni

; Carvalho, Werther Brunow de

; Odone Filho, Vicente

; Tannuri, Uenis

; Carneiro-Sampaio, Magda

; Grisi, Sandra

; Duarte, Alberto José da Silva

; Antonangelo, Leila

; Francisco, Rossana Pucineli Vieira

; Okay, Thelma Suely

; Batisttella, Linamara Rizzo

; Carvalho, Carlos Roberto Ribeiro de

; Brentani, Alexandra Valéria Maria

; Silva, Clovis Artur

; Eisencraft, Adriana Pasmanik

; Rossi Junior, Alfio

; Fante, Alice Lima

; Cora, Aline Pivetta

; Reis, Amelia Gorete A. de Costa

; Ferrer, Ana Paula Scoleze

; Andrade, Anarella Penha Meirelles de

; Watanabe, Andreia

; Gonçalves, Angelina Maria Freire

; Waetge, Aurora Rosaria Pagliara

; Silva, Camila Altenfelder

; Ceneviva, Carina

; Lazari, Carolina dos Santos

; Abellan, Deipara Monteiro

; Santos, Emilly Henrique dos

; Sabino, Ester Cerdeira

; Bianchini, Fabíola Roberta Marim

; Alcantara, Flávio Ferraz de Paes

; Ramos, Gabriel Frizzo

; Leal, Gabriela Nunes

; Rodriguez, Isadora Souza

; Pinho, João Renato Rebello

; Carneiro, Jorge David Avaizoglou

; Paz, Jose Albino

; Ferreira, Juliana Carvalho

; Ferranti, Juliana Ferreira

; Ferreira, Juliana de Oliveira Achili

; Framil, Juliana Valéria de Souza

; Silva, Katia Regina da

; Kanunfre, Kelly Aparecida

; Bastos, Karina Lucio de Medeiros

; Galleti, Karine Vusberg

; Cristofani, Lilian Maria

; Suzuki, Lisa

; Campos, Lucia Maria Arruda

; Perondi, Maria Beatriz de Moliterno

; Diniz, Maria de Fatima Rodrigues

; Fonseca, Maria Fernanda Mota

; Cordon, Mariana Nutti de Almeida

; Pissolato, Mariana

; Peres, Marina Silva

; Garanito, Marlene Pereira

; Imamura, Marta

; Dorna, Mayra de Barros

; Luglio, Michele

; Rocha, Mussya Cisotto

; Aikawa, Nadia Emi

; Degaspare, Natalia Viu

; Sakita, Neusa Keico

; Udsen, Nicole Lee

; Scudeller, Paula Gobi

; Gaiolla, Paula Vieira de Vincenzi

; Severini, Rafael da Silva Giannasi

; Rodrigues, Regina Maria

; Toma, Ricardo Katsuya

; Paula, Ricardo Iunis Citrangulo de

; Palmeira, Patricia

; Forsait, Silvana

; Farhat, Sylvia Costa Lima

; Sakano, Tânia Miyuki Shimoda

; Koch, Vera Hermina Kalika

; Cobello Junior, Vilson

Clinics

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2021, Volume 76 elocation e3488

Abstract: En | Text: En | PDF: En

OBJECTIVES: To compare demographic/clinical/laboratory/treatments and outcomes among children and adolescents with laboratory-confirmed coronavirus disease 2019 (COVID-19). METHODS: This was a cross-sectional study that included patients diagnosed with pediatric COVID-19 (aged <18 years) between April 11, 2020 and April 22, 2021. During this period, 102/5,951 (1.7%) of all admissions occurred in neonates, children, and adolescents. Furthermore, 3,962 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection samples were processed in patients aged <18 years, and laboratory-confirmed COVID-19 occurred in 155 (4%) inpatients and outpatients. Six/155 pediatric patients were excluded from the study. Therefore, the final group included 149 children and adolescents (n=97 inpatients and 52 outpatients) with positive SARS-CoV-2 results. RESULTS: The frequencies of sore throat, anosmia, dysgeusia, headache, myalgia, nausea, lymphopenia, pre-existing chronic conditions, immunosuppressive conditions, and autoimmune diseases were significantly reduced in children and adolescents (p<0.05). Likewise, the frequencies of enoxaparin use (p=0.037), current immunosuppressant use (p=0.008), vasoactive agents (p=0.045), arterial hypotension (p<0.001), and shock (p=0.024) were significantly lower in children than in adolescents. Logistic regression analysis showed that adolescents with laboratory-confirmed COVID-19 had increased odds ratios (ORs) for sore throat (OR 13.054; 95% confidence interval [CI] 2.750-61.977; p=0.001), nausea (OR 8.875; 95% CI 1.660-47.446; p=0.011), and lymphopenia (OR 3.575; 95% CI 1.355-9.430; p=0.010), but also had less hospitalizations (OR 0.355; 95% CI 0.138-0.916; p=0.032). The additional logistic regression analysis on patients with preexisting chronic conditions (n=108) showed that death as an outcome was significantly associated with pediatric severe acute respiratory syndrome (SARS) (OR 22.300; 95% CI 2.341-212.421; p=0.007) and multisystem inflammatory syndrome in children (MIS-C) (OR 11.261; 95% CI 1.189-106. 581; p=0.035). CONCLUSIONS: Half of the laboratory-confirmed COVID-19 cases occurred in adolescents. Individuals belonging to this age group had an acute systemic involvement of SARS-CoV-2 infection. Pediatric SARS and MIS-C were the most important factors associated with the mortality rate in pediatric chronic conditions with COVID-19.

https://doi.org/10.6061/clinics/2021/e3488

Diversity and spatio-temporal variation of Anopheles (Diptera: Culicidae) before and after the construction of the Jirau hydroelectric plant, state of Rondônia, Brazil
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Ferreira, Francisco Augusto da Silva

; Costa, Fábio Medeiros da

; Lima, Gervilane Ribeiro de

; Roque, Rosemary Aparecida

; Ferreira, Vania da Costa

; Santos Neto, Veríssimo Alves dos

; Benchimol, Thais Melo

; Scarpassa, Vera Margarete

; Tadei, Wanderli Pedro

Revista Brasileira de Entomologia

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2021, Volume 65 N. 2 elocation e20200105

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of this study was to evaluate the effect of the construction of the Jirau hydroelectric plant on the anopheline species, in Porto Velho, Rondônia, Brazil. For this, sampling was carried out in 23 locations in the pre (2004) and 23 after construction (2018) phases in the hydroelectric dam's coverage area, performed by human landing catch between 6:00 pm and 10:00 pm. We found 14 anopheline species. In the pre-construction phase, An. darlingi (73%) was the most abundant and after construction An. braziliensis (31.5%) was the most abundant. The T test indicated that there were no differences in the indexes – diversity (H '), richness (Sjack 1), equitability (J’) and dominance (d) of anophelines species - evaluated between the phases (p > 0.05). The spatio-temporal distribution of An. darlingi proved to be wide, with the highest density values observed in March ( x ¯w = 27.1) in the pre-construction phase and in the months of May ( x ¯w = 4) and July ( x ¯w = 3.1), post-construction. Differences in hematophagic activity peaks were observed between species and between phases. According to the data obtained in this study, the construction of the hydroelectric plant had little influence on the composition of anopheline species, however changes were observed in the spatio-temporal distribution of the An. darlingi and in the pattern of hematophagic activity of the species, which directly influenced the dynamics of malaria in the region.

https://doi.org/10.1590/1806-9665-rbent-2020-0105 148 downloads

10.

Physicochemical quality of brown soybean preserve in function of maceration time and ascorbic acid
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TASSI, Adriana Luiza Wain

; BENTO, Juliana Aparecida Correia

; CALIARI, Márcio

; SILVA, Vera Sônia Nunes da

; PACHECO, Maria Teresa Bertoldo

; VERA, Rosângela

; SOARES JÚNIOR, Manoel Soares

Food Science and Technology

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jun 2020, Volume 40 Pages 250 - 257

Abstract: En | Text: En | PDF: En

Abstract The aim of the present study was to evaluate the physical and chemical quality of the brown soybean preserve in function of the ascorbic acid concentration and the maceration time, and also to evaluate the chemical composition, microbiological hazard, and acceptability of the best preserve, in addition verify its technological, nutritional, functional and sensory viability. The hardness of the grain ranged between 8.6 and 23N and was significantly affected by the maceration time. The ascorbic acid concentration influenced antioxidant activity (ranged between 4.3 and 183.8 mg100g-1) and total phenolic compounds (ranged between 176.4 and 466.2 mg100g-1). The best brown soybean preserve was obtained with a maceration time of 23 min and an ascorbic acid concentration of 0.15 g100g-1 . This shows high nutritional value and essential amino acid content, absence of trypsin inhibitor and microbiological risk, and also sensory acceptance. Therefore, the brown soybean preserve is viable, and its consumption is recommended.

https://doi.org/10.1590/fst.09618 628 downloads

11.

Prevalence of anti-SARS-CoV-2 antibodies in outpatients of a large public university hospital in Sao Paulo, Brazil
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Oliveira, Luanda Mara da Silva

; Tiyo, Bruna Tiaki

; Silva, Lais Teodoro da

; Fonseca, Luiz Augusto Marcondes

; Rocha, Rosana Coura

; Santos, Vera Aparecida dos

; Ceneviva, Carina

; Bedin, Anderson Aparecido

; Almeida, Alexandre de

; Duarte, Alberto José da Silva

; Oshiro, Telma Miyuki

Revista do Instituto de Medicina Tropical de São Paulo

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2020, Volume 62 elocation e91

Abstract: En | Text: En | PDF: En

ABSTRACT Coronavirus disease 19 (COVID-19) is caused by SARS-Cov-2 and the manifestations of this infection range from an absence of symptoms all the way up to severe disease leading to death. To estimate the prevalence of past infection in a population, the most readily available method is the detection of antibodies against the virus. This study has investigated the prevalence of anti-SARS-CoV-2 antibodies in outpatients of the Hospital das Clinicas, in Sao Paulo city (Brazil), which is a large university hospital belonging to the public health system that cares for patients with complex diseases who need tertiary or quaternary medical care. Our serological inquiry was carried out for 6 weeks, with once-a-week blood sampling and included 439 patients from several outpatient services. Overall, 61 patients tested positive for anti-SARS-CoV-2 IgG (13.9%); 56.1 % of the patients live in Sao Paulo city, with the remaining living in other towns of the metropolitan area; 32.8% of the patients testing positive for IgG antibodies to SARS-CoV-2 were asymptomatic, 55.7% developed mild or moderate disease and 11.5% had to be hospitalized. The prevalence of SARS-CoV-2 positive serology was lower among patients who had received the seasonal influenza vaccine compared to the ones who did not. These findings may indicate that those individuals care more about health issues, and/or that they have a better access to health care and/or a better quality of health care service. The large proportion of patients who were unaware of having had contact with SARS-CoV-2 deserves attention, reflecting the scarcity of tests performed in the population.

https://doi.org/10.1590/s1678-9946202062091 1104 downloads

12.

Sensitivity of the Wondfo One Step COVID-19 test using serum samples
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Santos, Vera Aparecida dos

; Rafael, Mayra Matias

; Sabino, Ester Cerdeira

; Duarte, Alberto José da Silva

Clinics

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2020, Volume 75 elocation e2013

Abstract: En | Text: En | PDF: En

https://doi.org/10.6061/clinics/2020/e2013 5396 downloads

13.

Estratégias adaptativas foliares de Miconia nervosa (Melastomataceae) na Amazônia Matogrossense
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Müller, Angélica Oliveira

; Franco, Andréia Aparecida

; Ribeiro Júnior, Norberto Gomes

; Gressler, Eliana

; Rocha, Vera Lúcia Pegorini

; Silva, Ivone Vieira da

Rodriguésia

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2020, Volume 71 elocation e01052018

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A plasticidade fenotípica geralmente observada em caracteres morfológicos e anatômicos foliares pode ser influenciada por diversos fatores, como luminosidade, disponibilidade de nutrientes no solo, umidade e herbivoria, entre outros. Testamos essa plasticidade em Miconia nervosa (Melastomataceae) em um fragmento florestal no sul da Amazônia, Mato Grosso - Brasil, buscando verificar possíveis estratégias de aclimatação morfoanatômica à luminosidade. Coletamos folhas totalmente expandidas de 15 indivíduos adultos expostos diretamente à luz solar, nas margens de um lago, e 15 sob o sombreamento do dossel. Avaliamos quantitativamente oito variáveis morfológicas e seis anatômicas, das quais seis e quatro, respectivamente, diferiram significativamente entre folhas de sol e sombra. Morfologicamente, as folhas de sol apresentaram maior tamanho da lâmina foliar e do pecíolo e, anatomicamente, células epidérmicas com paredes levemente mais espessadas, maiores densidades e índice estomático. Folhas de sombra apresentaram epiderme significativamente mais espessa em ambas as faces. As folhas de M. nervosa apresentaram grau mediano a alto de plasticidade nas variáveis morfoanatômicas conforme índice de plasticidade fenotípica, com caracteres mistos de ambientes de sol e sombra. Sugerimos que a distribuição dos indivíduos de M. nervosa na floresta está relacionada à disponibilidade de luz e umidade do solo.

Abstract The phenotype plasticity usually observed in morphological and anatomical leaf traits may be influenced by several factors, such as luminosity, soil nutrient availability, humidity and herbivory, among others. We tested leaf plasticity in Miconia nervosa (Melastomataceae) in a forest fragment in Southern Amazonia, Mato Grosso State - Brazil, seeking to verify morpho-anatomical strategies of light acclimation. We collected fully expanded leaves of 15 individuals exposed directly to sunlight, on a lake edge, and 15 under canopy shading. We evaluated quantitatively eight morphological variables and six anatomical variables, of which six and four, respectively, differed significantly between sun and shade leaves. Morphologically, sun leaves showed larger leaf and petiole sizes and anatomically, epidermal cells with slightly thickened walls, higher stomatal index and density. Shade leaves showed significantly thicker epidermis on both leaf faces. Leaves of M. nervosa exhibited medium to high degree of plasticity in morpho-anatomical traits, according to the plasticity index, with mixed characteristics of sun and shade environments. We suggest that the distribution of M. nervosa individuals in the forest is related to the availability of light and soil moisture.

https://doi.org/10.1590/2175-7860202071094 354 downloads

14.

The ICF in the pedagogical projects of Physiotherapy courses in Midwest Brazil
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Fernandes, Juliana Aparecida Elias

; Gomes, Marília Miranda Forte

; Sousa, Bruna da Silva

; Romão, Juliana de Faria Fracon e

; Pinho, Diana Lúcia Moura

; Marães, Vera Regina Fernandes da Silva

Fisioterapia em Movimento

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2020, Volume 33 elocation e003344

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução: Os projetos políticos pedagógicos dos cursos de Fisioterapia (PPCs) no Brasil são concebidos à luz das Diretrizes Curriculares Nacionais do Curso de Graduação em Fisioterapia (DCNF) e aos princípios do SUS. Assim, os PPCs que norteiam a formação profissional tendem a incluir uma proposta de familiarização dos graduandos com a Classificação Internacional de Funcionalidade, Incapacidade e Saúde (CIF) com uma abordagem biopsicossocial, portanto os PPCs deveriam contemplar o uso da CIF. Objetivo: Verificar, por meio da análise exploratória dos PPCs, se existem propostas de ensino e uso da CIF na formação dos alunos. Método: Estudo quali-quantitativo, realizado por meio da análise documental exploratória dos PPCs dos cursos de Fisioterapia do Centro Oeste Brasileiro, dos quais foram extraídas informações de interesse relacionadas à CIF. Resultados: O modelo biopsicossocial e o delineamento das DCNF foram identificados nos 10 PPCs analisados e a CIF foi encontrada na matriz curricular de 6 PPCs, o que aponta positivamente para incorporação da CIF no processo formativo do aluno. Entretanto, somente em 4 PPCs foi possível identificá-la nos objetivos do curso e nas referências bibliográficas de disciplinas obrigatórias, a CIF foi encontrada em apenas 2 PPCs, o que pode indicar nesses documentos, uma possível fragilidade da abordagem da CIF. Conclusão: A presença da CIF em alguns PPCs indica mudanças e favorece o entendimento da funcionalidade, o que não anula a necessidade de uma maior abordagem quanto ao ensino da CIF nos demais PPCs, visando a formação do aluno dentro de um contexto biopsicossocial.

Abstract Introduction: The course pedagogical projects (CPPs) of physical therapy programs in Brazil are based on National Curriculum Guidelines for Physiotherapy (NCGP) and the principles of the National Health System (SUS). The CPPs that guide professional training tend to use a biopsychosocial approach and propose familiarizing undergraduate students with the International Classification of Functionality, Disability and Health (ICF); as such, they should include the use of this instrument. Objective: Assess CPPs by exploratory document analysis and determine whether they propose teaching and using the ICF in student training. Method: Qualitative-quantitative study with document analysis of CPPs for physical therapy courses in Midwest Brazil, from which information related to the ICF was extracted. Results: The biopsychosocial model and NGCP were identified in the 10 CPPs analyzed and the ICF was found in the curriculum outline of 6 of these, indicating the incorporation of this framework in student training. However, the ICF was only identified in the course objectives and literature references of 4 and 2 CPPs, respectively, suggesting possible shortcomings in its application in these documents. Conclusion: The inclusion of the ICF in some CPPs indicates a positive change and favors understanding of functioning, but does not preclude the need for a broader approach to teaching this classification framework in the remaining CPPs in order to provide student training within a biopsychosocial context.

https://doi.org/10.1590/1980-5918.033.ao44 637 downloads

15.

Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide
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Marciano, Maria Aparecida Moraes

; Silva, Rafaela Aparecida

; Barbosa, Maria Luisa

; Ferreira, Antônio Roberto Souza

; Pereira-Chioccola, Vera Lucia

Revista do Instituto de Medicina Tropical de São Paulo

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2020, Volume 62 elocation e84

Abstract: En | Text: En | PDF: En

ABSTRACT This study aimed to investigate a methodology for discriminating viable and non-viable T. gondii oocysts in water. Analyses included two steps: (i) microscopic investigation with vital dyes; (ii) molecular investigation, using a real time PCR (qPCR), after parasite treatment (or not) with propidium monoazide (PMA). The method was called qPCR-PMA. Oocyst aliquots were incubated (15 min) at 25 ºC or 100 ºC and analyzed by microscopy, after trypan blue and neutral red staining. Microscopic investigation determined viable and non-viable oocysts. For the molecular investigation, both aliquots of oocysts were treated with PMA. Non-viable oocysts, after PMA treatment, exhibited an inhibition of DNA amplification by qPCR. Although analyses were carried out with oocysts treated experimentally, these results suggest that qPCR-PMA can be a useful strategy to distinguish viable and non-viable T. gondii oocysts in water safety testing, showing if water is safe to drink.

https://doi.org/10.1590/s1678-9946202062084 530 downloads

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