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Abstract Introduction: Chronic kidney disease is usually asymptomatic, and its diagnosis depends on laboratory tests, with emphasis on serum creatinine and proteinuria. Objective: To assess knowledge on the role of serum creatinine as a biomarker of kidney function in a sample of the Brazilian population. Method: Cross-sectional observational study conducted in São Paulo (SP, Brazil), in which a random adult population was interviewed. Results: A total of 1138 subjects were interviewed, with a median age of 36 years old (27–52); 55.1% were female. Regarding the “creatinine” biomarker, 40.6% stated they had never performed such a test. When asked about their knowledge on the usefulness of this exam, only 19.6% knew its function. The other responses were “I don’t know” (71.6%), evaluating heart function (0.9%) and liver function (7.8%). Of those who reported they had already taken a creatinine test, only 29.4% correctly identified the role of creatinine. When dividing the groups into “knows” and “does not know” the function of creatinine, a statistically significant difference (p < 0.05) was observed regarding level of education, female sex, being a healthcare student/worker, having ever measured creatinine, knowing someone with kidney disease and older age. In the multivariate analysis, the main variable related to knowing the creatinine role was having previously taken the test (OR 5.16; 95% CI 3.16–8.43, p < 0.001). Conclusion: There is a significant lack of knowledge about creatinine and its use in checkups. The results indicate that greater efforts are needed from healthcare professionals to raise awareness on the role of serum creatinine. Introduction asymptomatic tests proteinuria Objective Method Crosssectional Cross sectional SP, SP (SP Brazil, Brazil , Brazil) interviewed Results 113 3 27–52 2752 27 52 (27–52) 551 55 1 55.1 “creatinine 406 40 6 40.6 exam 196 19 19.6 I dont don t know 71.6%, 716 71.6% 71 (71.6%) 0.9% 09 0 9 (0.9% 7.8%. 78 7.8% . 7 8 (7.8%) 294 29 4 29.4 knows “knows does 0.05 005 05 education sex studentworker student worker student/worker analysis OR 5.16 516 5 16 95 316843 43 3.16–8.43 0.001. 0001 0.001 001 0.001) Conclusion checkups 11 27–5 275 2 (27–52 55. 40. 19. 71.6 (71.6% 0.9 (0.9 7.8 (7.8% 29. 0.0 00 5.1 51 31684 3.16–8.4 000 0.00 27– (27–5 71. (71.6 0. (0. 7. (7.8 5. 3168 3.16–8. (27– (71. (0 (7. 316 3.16–8 (27 (71 ( (7 31 3.16– (2 3.16 3.1 3.

Resumo Introdução: A doença renal crônica costuma ser assintomática e seu diagnóstico depende da realização de exames laboratoriais, com destaque para a creatinina sérica e pesquisa de proteinúria. Objetivo: Avaliar em uma amostra da população brasileira o conhecimento sobre o papel da creatinina sérica como marcador de função renal. Método: Estudo observacional transversal realizado na cidade de São Paulo (SP, Brasil), em que foi entrevistada uma população adulta aleatória. Resultados: Foram entrevistados 1138 indivíduos, com idade mediana de 36 anos (27–52); 55,1% do sexo feminino. Com relação ao marcador “creatinina”, 40,6% afirmaram que nunca realizaram tal dosagem. Quando questionados quanto ao conhecimento sobre a utilidade desse exame, somente 19,6% sabiam a sua função. As outras respostas foram “não sei” (71,6%), avaliar o funcionamento do coração (0,9%) e fígado (7,8%). Dos que afirmaram já terem realizado o exame de creatinina, somente 29,4% acertaram a função da creatinina. Ao dividir os grupos em “sabe” e “não sabe” a função da creatinina, percebeu-se diferença estatisticamente significante (p < 0,05) em relação ao grau de escolaridade, sexo feminino, ser aluno/trabalhador da saúde, ter dosado creatinina alguma vez, conhecer alguém com doença renal e maior idade. Na análise multivariada, a principal variável relacionada com conhecer a função da creatinina foi ter realizado o exame anteriormente (OR 5,16; IC 95% 3,16–8,43, p < 0,001). Conclusão: Há grande desconhecimento sobre a creatinina e seu uso em check-ups. Os resultados indicam que é necessário maior esforço por parte dos profissionais de saúde para divulgar o papel da creatinina sérica. Introdução laboratoriais proteinúria Objetivo Método SP, SP (SP Brasil, Brasil , Brasil) aleatória Resultados 113 indivíduos 3 27–52 2752 27 52 (27–52) 551 55 1 55,1 feminino “creatinina” 406 40 6 40,6 dosagem 196 19 19,6 não sei 71,6%, 716 71,6% 71 (71,6%) 0,9% 09 0 9 (0,9% 7,8%. 78 7,8% . 7 8 (7,8%) 294 29 4 29,4 sabe “sabe percebeuse percebeu se 0,05 005 05 escolaridade alunotrabalhador aluno trabalhador vez multivariada OR 5,16 516 5 16 95 316843 43 3,16–8,43 0,001. 0001 0,001 001 0,001) Conclusão checkups. checkups check ups. ups check-ups 11 27–5 275 2 (27–52 55, “creatinina 40, 19, 71,6 (71,6% 0,9 (0,9 7,8 (7,8% 29, 0,0 00 5,1 51 31684 3,16–8,4 000 0,00 27– (27–5 71, (71,6 0, (0, 7, (7,8 5, 3168 3,16–8, (27– (71, (0 (7, 316 3,16–8 (27 (71 ( (7 31 3,16– (2 3,16 3,1 3,

https://doi.org/10.1590/2175-8239-jbn-2023-0117en

Consensus of the Brazilian Association of Hematology, Hemotherapy and Cellular Therapy (ABHH) and the Brazilian Ministry of Health - General management of blood and blood products on the tests necessary for the release of exceptional medicines for sickle cell disease Hematology ABHH (ABHH
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Lobo, Clarisse

; Araújo, Aderson

; Antunes, Alexandre de Albuquerque

; Pinto, Ana Cristina Silva

; Godinho, Ariadne Carvalho

; Pires, Cassia Silvestre Mariano

; Matheus, Cinthia Cristina

; Albuquerque, Xerez de

; Neves, Daniele Campos Fontes

; Moreno, Fábio de Lima

; Baldanzi, Giorgio

; Siufi, Grazziella Curado

; Miranda, Heloisa Helena Pereira

; Hankins, Jane

; Aragão, Joice

; Braga, Josefina Aparecida Pellegrini

; Martins, Juliana Touguinha Neves

; Souza, Luciana Campos Costa Machado de

; Figueiredo, Maria Stella

; Oliveira, Mirella Rodrigues

; Cardoso, Patricia Santos Resende

; Pinto, Patricia Costa Alves

; Moura, Patricia Gomes

; Cançado, Rodolfo Delfini

; Araujo, Paulo Ivo Cortez de

; Saad, Sara Olalla

; Loggetto, Sandra Regina

; Fonseca, Teresa Cristina Cardoso

Hematology, Transfusion and Cell Therapy

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mar 2024, Volume 46 N. 1 Pages 67 - 71

Abstract: En | Text: En | PDF: En

Abstract To date, hydroxyurea is the only effective and safe drug that significantly reduces morbidity and mortality of individuals with Sickle cell disease. Twenty years of real-life experience has demonstrated that hydroxyurea reduces pain attacks, vaso-occlusive events, including acute chest syndrome, the number and duration of hospitalizations and the need for transfusion. The therapeutic success of hydroxyurea is directly linked to access to the drug, the dose used and adherence to treatment which, in part, is correlated to the availability of hydroxyurea. This consensus aims to reduce the number of mandatory exams needed to access the drug, prioritizing the requesting physician's report, without affecting patient safety. date disease reallife real life attacks vasoocclusive vaso occlusive events syndrome transfusion which part physicians physician s report safety

https://doi.org/10.1016/j.htct.2024.01.001

IL-1β and IL-17 in cutaneous lupus erythematous skin biopsies: could immunohistochemicals indicate a tendency towards systemic involvement? IL1β ILβ IL 1β β IL17 17 IL-1 biopsies involvement IL1 1 IL-
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Lovato, Barbara Hartung

; Fogagnolo, Leticia

; Souza, Elemir Macedo de

; Silva, Larissa Juliana Batista da

; Velho, Paulo Eduardo Neves Ferreira

; Cintra, Maria Leticia

; Teixeira, Fernanda

Anais Brasileiros de Dermatologia

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jan 2024, Volume 99 N. 1 Pages 66 - 71

Abstract: En | Text: En | PDF: En

Abstract Background: Only a fraction of patients with cutaneous lupus erythematosus (CLE) will eventually progress toward systemic disease (SLE). Objective: To find inflammatory biomarkers which could predict the progression of cutaneous lupus erythematosus (CLE) into systemic lupus erythematosus (SLE) using immunohistochemical (IHC) assays. Methods: Immunohistochemical markers for cytotoxic, inflammatory, and anti-inflammatory responses and morphometric methods were applied to routine paraffin sections of skin biopsies, taken from lesions of 59 patients with discoid lupus, subacute lupus, and lupus tumidus. For the diagnosis of SLE, patients were classified by both the American College of Rheumatology (ACR-82) and the Systemic Lupus International Collaborating Clinics (SLICC-12) systems. Results: Skin samples from CLE/SLE +patients presented higher expression of IL-1β (ARC-82: p = 0.024; SLICC-12: p = 0.0143) and a significantly higher number of cells marked with granzyme B and perforin (ARC: p = 0.0097; SLICC-12: p = 0.0148). Biopsies from CLE/SLE- individuals had higher expression of IL-17 (ARC-82: p = 0.0003; SLICC-12: p = 0.0351) and presented a positive correlation between the density of granzyme A+and FoxP3+ cells (ARC-82: p = 0.0257; SLICC-12: p = 0.0285) and CD8+ cells (ARC-82: p = 0.0075; SLICC-12: p = 0.0102), as well as between granulysin-positive and CD8+ cells (ARC-82: p = 0.0024; SLICC-12: p = 0.0116). Study limitations: Patients were evaluated at a specific point in their evolution and according to the presence or not of systemic disease. The authors cannot predict how many more, from each group, would have evolved towards SLE in the following years. Conclusions: In this cohort, immunohistochemical findings suggested that patients with a tendency to systemic disease will show strong reactivity for IL-1β, while those with purely cutaneous involvement will tend to express IL-17 more intensely. Background CLE (CLE SLE. . Objective (SLE IHC (IHC assays Methods cytotoxic antiinflammatory anti biopsies 5 tumidus ACR82 ACR 82 (ACR-82 SLICC12 SLICC 12 (SLICC-12 systems Results CLESLE IL1β ILβ IL 1β β ARC82 ARC (ARC-82 0.024 0024 0 024 SLICC-12 0.0143 00143 0143 (ARC 0.0097 00097 0097 0.0148. 00148 0.0148 0148 0.0148) IL17 17 IL-1 0.0003 00003 0003 0.0351 00351 0351 Aand A FoxP3 FoxP 0.0257 00257 0257 0.0285 00285 0285 CD8 CD 0.0075 00075 0075 0.0102, 00102 0.0102 , 0102 0.0102) granulysinpositive granulysin 0.0024 00024 0.0116. 00116 0.0116 0116 0.0116) limitations group years Conclusions cohort IL1β, 1β, intensely ACR8 8 (ACR-8 SLICC1 1 (SLICC-1 ARC8 (ARC-8 0.02 002 02 SLICC-1 0.014 0014 014 0.009 0009 009 IL1 IL- 0.000 0000 000 0.035 0035 035 0.025 0025 025 0.028 0028 028 0.007 0007 007 0010 0.010 010 0.002 0002 0011 0.011 011 (ACR- (SLICC- (ARC- 0.0 00 SLICC- 0.01 001 01 0.00 0.03 003 03 (ACR (SLICC 0.

https://doi.org/10.1016/j.abd.2023.02.007

Understanding of the role of serum creatinine in a subset of the Brazilian population
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Cerqueira, Bruno Pellozo

; Rocha, Júlia Ferreira

; Barnes, Rafaela Francisquetti

; Pepato, Pedro Henrique Moretti

; Paim, Thays Sellan

; De Nardi, Francisco

; Hsu, Fabrício Akira

; Oguma, Juliana Miki

; Ito, Leticia Miyuki

; da Silva, Enio Yasuhiro Arimatsu Policarpo

; Vizzuso-Oliveira, Alexandre

; Filho, Fernando Diniz dos Santos

; Maia, João Vitor Bozza

; Mendez, Juan Diego Zambrano

; Sato, Beatrice Borges

; Souza, Roberto Matias

; Miyahara, Andre Kiyoshi

; Kirsztajn, Gianna Mastroianni

Brazilian Journal of Nephrology

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2024, N. ahead

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

https://doi.org/10.1590/2175-8239-jbn-2023-0117en

Psychometric properties of the Providers Survey in the Brazilian context of mental health: a validation study health
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Lacerda, Mayara Karoline Silva

; Vieira, Maria Aparecida

; Marques, Fabrine Costa

; Alves, Juliana Pereira

; Pereira, Matheus Mendes

; Carmo, Andreia Cristina Feitosa do

; Costa, Mark Napoli

; Caldeira, Antônio Prates

; Sampaio, Cristina Andrade

Sao Paulo Medical Journal

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2024, Volume 142 N. 2 elocation e2022444

Abstract: En | Text: En | PDF: En

ABSTRACT BACKGROUND: Precisely determining the aspects related to an instrument's validity and reliability measures allows for greater assurance of the quality of the results. OBJECTIVES: To analyze the psychometric properties of The Providers Survey in the Brazilian context of mental health services. DESIGN AND SETTING: The instrument validation study was conducted in Montes Claros, Minas Gerais, Brazil. METHODS: The validation study was conducted using the Consensus-based Standards for the Selection of Health Measurement Instruments checklist to analyze its validity and reliability. RESULTS: A committee of expert judges performed content validation after which the Content Validity Index was calculated. Construct validation took place through Exploratory Factor Analysis using the Kaiser-Meyer-Olkin Test criterion and Bartlett's Sphericity Test. Reliability was verified using test-retest reliability. The significance level adopted for the statistical tests was 5% (P < 0.05). The final instrument comprised 54 questions. The Content Validity Index was 97%. Exploratory Factor Analysis identified a Kaiser-Meyer-Olkin index of 0.901 and Bartlett's Sphericity Test with P < 0.001. We obtained a Cronbach's alpha coefficient of 0.95 and an intraclass correlation coefficient of 0.849. CONCLUSIONS: The Providers Survey, translated and adapted into Portuguese, was named the Work Assessment Instrument for the Recovery of Mental Health. It presented adequate psychometric properties for evaluating work-related practices for the recovery of psychosocial care network users. BACKGROUND instruments s results OBJECTIVES services SETTING Claros Gerais Brazil METHODS Consensusbased Consensus based RESULTS calculated KaiserMeyerOlkin Kaiser Meyer Olkin Bartletts Bartlett testretest test retest 5 0.05. 005 0.05 . 0 05 0.05) questions 97 97% 0901 901 0.90 0001 001 0.001 Cronbachs Cronbach 095 95 0.9 0849 849 0.849 CONCLUSIONS Portuguese workrelated work users 00 0.0 9 090 90 000 0.00 09 0. 084 84 0.84 08 8 0.8

https://doi.org/10.1590/1516-3180.2022.0444.r3.010623

Desenvolvimento de um design de telessimulação para o suporte básico de vida
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Bernardinelli, Fabiana Cristina Pires

; Amorim, Gustavo Correa de

; Nascimento, Juliana da Silva Garcia

; Fonseca, Luciana Mara Monti

; Domingues, Tânia Arena Moreira

; Condeles, Paulo César

; Chavaglia, Suzel Regina Ribeiro

Acta Paulista de Enfermagem

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2024, Volume 37 elocation eAPE00021

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen Objetivo Elaborar y validar un diseño de telesimulación sincrónica y observacional de enfermería para la atención de acuerdo con el protocolo de soporte vital básico intrahospitalario en adultos. Métodos Estudio metodológico basado en las fases del procedimiento teórico realizado por medio de una scoping review, fase empírica llevada cabo a través de la validación de contenido del diseño telesimulado y fase analítica en la que se adoptó el índice de validez de contenido. Resultados Se elaboró un diseño para la atención de acuerdo con el protocolo de soporte vital básico que atravesó seis etapas: planificación, preparación, participación, teledebriefing, evaluación y feedback y aprendizaje adicional, con un Índice de Validez de Contenido de 0,96. Conclusión El diseño elaborado fue considerado válido en contenido para planificar y ejecutar la telesimulación por parte de enfermeros y además puede adaptarse a otros contextos educativos. adultos review etapas planificación preparación participación teledebriefing adicional 096 0 96 0,96 educativos 09 9 0,9 0,

Abstract Objective To construct and validate a synchronous and observational telesimulation design in nursing for care according to in-hospital basic life support protocol for adults. Methods A methodological study based on the phases of the theoretical procedure carried out through a scoping review; empirical phase developed through the telesimulated design content validity and analytical phase in which the content validity index was adopted. Results A care design was constructed according to the basic life support protocol, going through six steps: planning, preparation, participation, teledebriefing, assessment and feedback and additional learning, with a Content Validity Index of 0.96. Conclusion The developed design was considered valid in content to plan and execute telesimulation by nursing and still be adapted to other educational contexts. inhospital hospital adults review adopted steps planning preparation participation teledebriefing learning 096 0 96 0.96 contexts 09 9 0.9 0.

Resumo Objetivo Construir e validar um design de telessimulação síncrona e observacional em enfermagem para o atendimento segundo o protocolo de suporte básico de vida intra-hospitalar no adulto. Métodos Estudo metodológico baseado nas fases do procedimento teórico realizado por meio de uma scoping review; fase empírica desenvolvida através da validação de conteúdo do design telessimulado e fase analítica em que adotou-se o índice de validade de conteúdo. Resultados Construiu-se um design para o atendimento segundo o protocolo de suporte básico de vida percorrendo seis etapas: o planejamento, preparação, participação, teledebriefing, avaliação e feedback e a aprendizagem adicional, com Índice de Validade de Conteúdo de 0,96. Conclusão O design desenvolvido foi considerado válido em conteúdo para planejar e executar a telessimulação pela enfermagem e ainda ser adaptado a outros contextos educacionais. intrahospitalar intra hospitalar adulto review adotouse adotou se Construiuse Construiu etapas planejamento preparação participação teledebriefing adicional 096 0 96 0,96 educacionais 09 9 0,9 0,

https://doi.org/10.37689/acta-ape/2024ao0000021

Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses maize
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Koltun, Alessandra

; Silva, Priscila Alves da

; Torres, Isabela Yamauchi

; Bonifácio-Anacleto, Fernando

; Yassitepe, Juliana Erika de Carvalho Teixeira

Crop Breeding and Applied Biotechnology

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2024, Volume 24 N. 1 elocation e46392411

Abstract: En | Text: En | PDF: En

Abstract Microsatellites have been widely used to genotype individuals and to address a myriad of biological questions in many research fields for decades. However, when implementing a microsatellite marker analysis routine from scratch, various problems can arise throughout the process from DNA extraction to allele scoring, including inputting errors in the database, decreasing the reliability of results and having profound negative impacts on the derived decisions. Therefore, correctly assigning a genotype to a sample is crucial and dependent on acquiring knowledge of the technique steps, such as the chemistry of reactions, software, and data curation. This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field. decades However scratch scoring database decisions Therefore steps reactions software curation SSR (SSR tenplex plex (ten-plex 114 genotypes Here microsatellitebased based them field 11 1

https://doi.org/10.1590/1984-70332024v24n1a01

Lung cancer screening in Brazil: recommendations from the Brazilian Society of Thoracic Surgery, Brazilian Thoracic Association, and Brazilian College of Radiology and Diagnostic Imaging Brazil Surgery Association
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Pereira, Luiz Fernando Ferreira

; Santos, Ricardo Sales dos

; Bonomi, Daniel Oliveira

; Franceschini, Juliana

; Santoro, Ilka Lopes

; Miotto, André

; Sousa, Thiago Lins Fagundes de

; Chate, Rodrigo Caruso

; Hochhegger, Bruno

; Gomes Neto, Artur

; Schneider, Airton

; Araújo Neto, César Augusto de

; Escuissato, Dante Luiz

; Prado, Gustavo Faibischew

; Costa-Silva, Luciana

; Zamboni, Mauro Musa

; Ghefter, Mario Claudio

; Corrêa, Paulo César Rodrigues Pinto

; Torres, Pedro Paulo Teixeira e Silva

; Mussi, Ricardo Kalaf

; Muglia, Valdair Francisco

; Godoy, Irma de

; Bernardo, Wanderley Marques

Jornal Brasileiro de Pneumologia

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2024, Volume 50 N. 1 elocation e20230233

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Although lung cancer (LC) is one of the most common and lethal tumors, only 15% of patients are diagnosed at an early stage. Smoking is still responsible for more than 85% of cases. Lung cancer screening (LCS) with low-dose CT (LDCT) reduces LC-related mortality by 20%, and that reduction reaches 38% when LCS by LDCT is combined with smoking cessation. In the last decade, a number of countries have adopted population-based LCS as a public health recommendation. Albeit still incipient, discussion on this topic in Brazil is becoming increasingly broad and necessary. With the aim of increasing knowledge and stimulating debate on LCS, the Brazilian Society of Thoracic Surgery, the Brazilian Thoracic Association, and the Brazilian College of Radiology and Diagnostic Imaging convened a panel of experts to prepare recommendations for LCS in Brazil. The recommendations presented here were based on a narrative review of the literature, with an emphasis on large population-based studies, systematic reviews, and the recommendations of international guidelines, and were developed after extensive discussion by the panel of experts. The following topics were reviewed: reasons for screening; general considerations about smoking; epidemiology of LC; eligibility criteria; incidental findings; granulomatous lesions; probabilistic models; minimum requirements for LDCT; volumetric acquisition; risks of screening; minimum structure and role of the multidisciplinary team; practice according to the Lung CT Screening Reporting and Data System; costs versus benefits of screening; and future perspectives for LCS. LC (LC tumors 15 stage 85 cases (LCS lowdose low dose (LDCT LCrelated related 20 20% 38 cessation decade populationbased population recommendation incipient necessary Surgery Association literature studies reviews guidelines reviewed criteria findings lesions models acquisition team System 1 8 2 3

RESUMO O câncer de pulmão (CP) é uma das neoplasias mais comuns e letais no Brasil, e apenas 15% dos pacientes são diagnosticados nos estágios iniciais. O tabagismo persiste como o responsável por mais de 85% de todos os casos. O rastreamento do CP (RCP) por meio da TC de baixa dosagem de radiação (TCBD) reduz a mortalidade do CP em 20%, e, quando combinado com a cessação do tabagismo, essa redução chega a 38%. Na última década, diversos países adotaram o RCP como recomendação de saúde populacional. No Brasil, embora ainda incipiente, a discussão sobre o tema é cada vez mais ampla e necessária. Com o intuito de aumentar o conhecimento e estimular o debate sobre o RCP, a Sociedade Brasileira de Cirurgia Torácica, a Sociedade Brasileira de Pneumologia e Tisiologia e o Colégio Brasileiro de Radiologia e Diagnóstico por Imagem constituíram um painel de especialistas para elaborar as recomendações para o RCP. As recomendações aqui apresentadas foram baseadas em revisão narrativa da literatura, com ênfase em grandes estudos populacionais, em revisões sistemáticas e em recomendações de diretrizes internacionais, sendo construídas após ampla discussão pelo grupo de especialistas. Os temas revisados foram os seguintes: porque rastrear, considerações gerais sobre tabagismo, epidemiologia do CP, critérios de elegibilidade, achados incidentais, lesões granulomatosas, modelos probabilísticos, requisitos mínimos da TCBD, aquisições volumétricas, riscos do rastreamento, estrutura mínima e papel da equipe multidisciplinar, conduta segundo o Lung CT Screening Reporting and Data System (Lung-RADS), custos vs. benefícios e perspectivas do rastreamento. (CP Brasil 15 iniciais 85 casos (RCP TCBD (TCBD 20 20% 38 38% década populacional incipiente necessária Torácica literatura populacionais internacionais seguintes rastrear elegibilidade incidentais granulomatosas probabilísticos volumétricas multidisciplinar LungRADS, LungRADS RADS , (Lung-RADS) vs 1 8 2 3 (Lung-RADS

https://doi.org/10.36416/1806-3756/e20230233

Poly-ε-caprolactone based nanoparticles for delivery of genistein in melanoma treatment Polyεcaprolactone Poly ε caprolactone
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Abriata, Juliana Palma

; Luiz, Marcela Tavares

; Viegas, Juliana Santos Rosa

; Fumagalli, Fernando

; Melo, Shaiani Maria Gilde

; Emery, Flavio da Silva

; Marchetti, Juliana Maldonado

; Vicentini, Fabiana Testa Moura de Carvalho

Brazilian Journal of Pharmaceutical Sciences

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2024, Volume 60 elocation e22542

Abstract: En | Text: En | PDF: En

Abstract We developed poly-ε-caprolactone (PCL)-based nanoparticles containing D-α-tocopherol polyethylene glycol-1000 succinate (TPGS) or Poloxamer 407 as stabilizers to efficiently encapsulate genistein (GN). Two formulations, referred to as PNTPGS and PNPol, were prepared using nanoprecipitation. They were characterized by size and PDI distribution, zeta potential, nanoparticle tracking analysis (NTA), GN association (AE%), infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC). PNTPGS-GN exhibited a particle size of 141.2 nm, a PDI of 0.189, a zeta potential of -32.9 mV, and an AE% of 77.95%. PNPol-GN had a size of 146.3 nm, a better PDI than PNTPGS-GN (0.150), a less negative zeta potential (-21.0 mV), and an AE% of 68.73%. Thermal and spectrometric analyses indicated that no new compounds were formed, and there was no incompatibility detected in the formulations. Cellular studies revealed that Poloxamer 407 conferred less toxicity to PCL nanoparticles. However, the percentage of uptake decreased compared to the use of TPGS, which exhibited almost 80% cellular uptake. This study contributes to the investigation of stabilizers capable of conferring stability to PCL nanoparticles efficiently encapsulating GN. Thus, the PCL nanoparticle proposed here is an innovative nanomedicine for melanoma therapy and represents a strong candidate for specific pre-clinical and in vivo studies. polyεcaprolactone poly ε caprolactone PCLbased based Dαtocopherol D α tocopherol glycol1000 glycol 1000 glycol-100 TPGS (TPGS 40 . (GN) formulations PNPol nanoprecipitation distribution NTA, NTA , (NTA) AE%, AE (AE%) FTIR, FTIR FT IR (FT-IR) DSC. DSC (DSC) PNTPGSGN 1412 141 2 141. nm 0189 0 189 0.189 32.9 329 32 9 -32. mV 7795 77 95 77.95% PNPolGN 1463 146 3 146. 0.150, 0150 0.150 150 (0.150) 21.0 210 21 (-21. mV) 6873 68 73 68.73% formed However 80 Thus preclinical pre clinical glycol100 100 glycol-10 4 (GN (NTA (AE% (FT-IR (DSC 14 018 18 0.18 32. -32 779 7 77.95 015 0.15 15 (0.150 21. (-21 687 6 68.73 8 glycol10 10 glycol-1 (AE 1 01 0.1 -3 77.9 (0.15 (-2 68.7 glycol1 glycol- 0. - 77. (0.1 (- 68. (0. ( (0

https://doi.org/10.1590/s2175-97902024e22542

10.

Does tourism policy consider climate? Exploring rainy season narratives on the Alagoas coast, Brazil climate coast
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Bombana, Briana

; Martins, Ivan Machado

; Gaeta, Juliana de Carvalho

; Pereira, Marcos Paulo Santos

; Normande, Iran Campello

; Cerqueira, Aline da Silva

Ambiente & Sociedade

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2024, Volume 27 elocation e00108

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen Este estudio buscó expandir la discusión sobre la extensión en que el turismo puede responder a las expectativas socioeconómicas. Como en otras zonas costeras, el estado de Alagoas ha priorizado la promoción turística, pese a la ocurrencia de una estación lluviosa que frena el desarrollo de su principal tipo de turismo, de sol y playa. En este contexto, clima y tiempo son utilizados como puntos de referencia para este análisis. Por intermedio de diversas fuentes, se identificaron nueve narrativas sobre la estación lluviosa. Al cruzarlas con el Plan Estratégico para el Desarrollo Turístico de Alagoas 2013-2023, los resultados destacaron cómo la estación lluviosa está mal reflejada en dicha política pública. Para mejor abordar las limitaciones y demandas locales-regionales actuales, son propuestas recomendaciones. Más allá de esto, el abordaje de las narrativas probó su utilidad para expandir la consideración de valores y significados en políticas públicas, potencialmente en la relación ambiente-sociedad. socioeconómicas costeras turística playa contexto análisis fuentes 20132023, 20132023 2013 2023, 2023 2013-2023 pública localesregionales locales regionales actuales recomendaciones esto públicas ambientesociedad. ambientesociedad ambiente sociedad. sociedad ambiente-sociedad 2013202 201 202 2013-202 201320 20 2013-20 20132 2 2013-2 2013-

Abstract This study aims to expand the discussion regarding the extent to which tourism can address socioeconomic expectations. Like other coastal regions, the state of Alagoas has prioritised tourism promotion, despite facing a rainy season that hinders its primary form of tourism, known as Sea, Sun and Sand tourism. We have based our assessment on climate and weather. Through various sources, we have identified narratives related to the rainy season. When crossing these narratives with the Alagoas Strategic Plan for Tourism Development 2013-2023, our findings revealed that the state’s policy inadequately addresses the impact of the rainy season. Consequently, we have proposed some recommendations to better align with current local-regional constraints and demands. Beyond the scope of this work, our narrative approach has proven valuable in expanding the consideration of values and meanings within the framework of public policy development, potentially fostering a more holistic understanding of the environment-society relationship. expectations regions promotion Sea weather sources 20132023, 20132023 2013 2023, 2023 2013-2023 states s Consequently localregional local regional demands work development environmentsociety environment society relationship 2013202 201 202 2013-202 201320 20 2013-20 20132 2 2013-2 2013-

Resumo Esse estudo busca ampliar a discussão sobre a extensão na qual o turismo pode responder às expectativas socioeconômicas. Como em outras zonas costeiras, o estado de Alagoas tem priorizado a promoção turística, apesar da ocorrência de uma estação chuvosa que freia o desenvolvimento do seu principal tipo de turismo, de sol e praia. Neste contexto, o clima e o tempo são utilizados como pontos de referência para essa análise. Por intermédio de diversas fontes, foram identificadas nove narrativas relacionadas à estação chuvosa. As narrativas foram cruzadas com o Plano Estratégico para o Desenvolvimento Turístico de Alagoas 2013-2023, apontando que a estação chuvosa está mal refletida na política estadual. Para melhor abordar as limitações e demandas locais-regionais atuais, recomendações foram, assim, propostas. Além do escopo desse trabalho, a abordagem das narrativas provou ser útil para ampliar a consideração de valores e significados no desenvolvimento de políticas públicas, especialmente na relação ambiente-sociedade. socioeconômicas costeiras turística praia contexto análise fontes 20132023, 20132023 2013 2023, 2023 2013-2023 estadual locaisregionais locais regionais atuais assim propostas trabalho públicas ambientesociedade. ambientesociedade ambiente sociedade. sociedade ambiente-sociedade 2013202 201 202 2013-202 201320 20 2013-20 20132 2 2013-2 2013-

https://doi.org/10.1590/1809-4422asoc0108r1vu27l2oa

11.

Confinement and semi confinement of lambs in an integrated production system: impacts on production performance, infection by gastrointestinal nematodes, and selected blood analytes system performance nematodes
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Schmidt, Elizabeth Moreira dos Santos

; Fachiolli, Daniele Floriano

; Meirelles, Paulo Roberto de Lima

; Soutello, Ricardo Velludo Gomes de

; Pariz, Cristiano Magalhães

; Castilhos, André Michel de

; Amarante, Alessandro Francisco Talamini do

; Almeida, Fabiana Alves de

; Souza, Daniel Martins de

; Barros, Juliana da Silva

; Costa, Ciniro

Ciência Rural

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2024, Volume 54 N. 8 elocation e20230406

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT: This trial evaluated the production performance, gastrointestinal nematode infection (GIN), and selected blood analytes, in Corriedale lambs during the finishing phase in an integrated crop-livestock system (ICLS) with 2 stocking rates. Fifty-two Corriedale lambs were divided into 4 groups: a semi confinement system with a low stocking rate (T1) for 70 days; a semi confinement system with a high stocking rate (T2) for 70 days; confinement for 28 days, followed by semi confinement with a low stocking rate (T3) for 42 days; and confinement for 28 days, followed by semi confinement with a high stocking rate (T4) for 42 days. Body weight, dry matter intake, blood collection, and faecal analysis of nematode eggs were performed for each animal. The stocking rates did not influence body weight gain for the semiconfined lambs (T1 and T2) or for the confined/semiconfined lambs (T3 and T4). All treatments had lower EPG counts at the end of the experimental period. The nematode genera identified during the experiment trial were Haemonchus and Trichostrongylus. There were no significant differences (P > 0.05) for packed cell volume, total plasma protein, and eosinophil means between treatments. These results suggested that the confinement followed by semi confinement in an ICLS, was an option for lamb production during the finishing phase which could be useful in reducing GIN without affecting the production performance. ABSTRACT performance GIN, , (GIN) analytes croplivestock crop livestock ICLS (ICLS Fiftytwo Fifty two groups T1 T 7 days T2 (T2 T3 T4 (T4 intake collection animal (T confinedsemiconfined confined T4. . T4) period Trichostrongylus P 0.05 005 0 05 volume protein (GIN 0.0 00 0.

RESUMO: O objetivo deste experimento foi avaliar o desempenho produtivo, infecção por nematódeos gastrointestinais (GIN) e variáveis sanguíneas selecionadas, em cordeiras Corriedale durante a fase de terminação em sistema integrado lavoura-pecuária (ICLS) com duas taxas de lotação. O experimento utilizou 52 cordeiras Corriedale divididas em quatro grupos: sistema de semiconfinamento com baixa taxa de lotação por área (T1) por 70 dias; sistema de semiconfinamento com alta lotação por área (T2) por 70 dias; confinamento por 28 dias, seguido de semiconfinamento com baixa lotação por área (T3) por 42 dias; e confinamento por 28 dias, seguido de semiconfinamento com alta lotação por área (T4) por 42 dias. Peso corporal, ingestão de matéria seca, coleta de sangue e análise fecal para ovos de nematódeos foram avaliados para cada animal. As taxas de lotação não influenciaram no ganho de peso dos cordeiros semiconfinados (T1 e T2) ou nos confinados/semiconfinados (T3 e T4). Todos os tratamentos apresentaram contagens menores de EPG no final do período experimental. Os gêneros de nematódeos identificados durante o experimento foram Haemonchus e Trichostrongylus. Não houve diferença significativa (P > 0.05) para hematócrito, proteína plasmática total e média de eosinófilos entre os tratamentos. Esses resultados sugerem que o confinamento seguido de semiconfinamento em ICLS pode ser uma opção para a produção de cordeiras na fase de terminação e pode ser útil para a redução da GIN sem afetar o desempenho da produção. RESUMO produtivo (GIN selecionadas lavourapecuária lavoura pecuária (ICLS 5 grupos T1 T 7 dias T2 (T2 2 T3 4 T4 (T4 corporal seca animal (T confinadossemiconfinados confinados T4. . T4) experimental Trichostrongylus P 0.05 005 0 05 hematócrito 0.0 00 0.

https://doi.org/10.1590/0103-8478cr20230406

12.

Substrates and containers influence the growth of Campomanesia phaea (O. Berg. Landrum) seedlings, an endangered Atlantic Rainforest species O. O (O Berg Landrum seedlings
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Santoro, Marcelo Brossi

; Gomes, Juliana Aparecida Souza

; Brogio, Bruna do Amaral

; Jacomino, Angelo Pedro

; Silva, Simone Rodrigues da

Acta Scientiarum. Agronomy

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2024, Volume 46 elocation e63904

Abstract: En | Text: En | PDF: En

ABSTRACT. Native to the Brazilian Atlantic Rainforest, cambuci fruits can be consumed either in natura or in the processed form, and cambuci trees can be used to recover degraded areas. However, studies regarding cambuci germination and seedling development are scarce. The main aim of this study was to understand how different substrates and containers influence the growth of cambuci seedlings. To this end, two experiments were conducted: five different substrates were tested in the first experiment, and based on the multivariate analysis of the first experiment, the effect of combination of the best-performing substrates and three commonly employed plant growth containers on seedling development was investigated. Overall, good quality cambuci seedlings were obtained when they were grown in the commercial pine bark substrate and peat and perlite mixture in a 260 cm3 container. ABSTRACT Rainforest form areas However scarce end conducted experiment bestperforming best performing investigated Overall 26 cm container 2

https://doi.org/10.4025/actasciagron.v46i1.63904

13.

Severe esophagitis induced by antituberculosis drugs: a case report drugs
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Mamani, Roxana Flores

; Silva, Franciele Moreira

; Lima-Júnior, Marcos Vidal de

; Lima, Juliana Paitach de Oliveira

; Vianna, Vitor Montez

; Azevedo, Rivelino Trindade de

; McBenedict, Billy

; Martins, Ezequias Batista

Revista do Instituto de Medicina Tropical de São Paulo

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2024, Volume 66 elocation e2

Abstract: En | Text: En | PDF: En

ABSTRACT Tuberculosis stands as one of humanity’s oldest afflictions, intrinsically intertwined with social disparities. This formidable disease spares no age group and remains the prevailing cause of infection-induced mortality worldwide, particularly in developing nations. We present a case of a 56-year-old woman with diabetes who was diagnosed with Pulmonary Tuberculosis. After receiving antituberculosis drugs as part of her treatment, she experienced a range of systemic manifestations and suffered from severe ulcerative esophagitis. This adverse reaction led to uncontrollable gastrointestinal intolerance, tragically resulting in her untimely demise. The incident underscores the potential seriousness of adverse reactions that can arise from tuberculosis treatment medications. humanitys humanity s afflictions disparities infectioninduced infection induced worldwide nations 56yearold yearold 56 year old esophagitis intolerance demise medications 5

https://doi.org/10.1590/s1678-9946202466002

14.

Neurological manifestation of Brazilian spotted fever in childhood
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Mendes, Bruna Fernanda Deicke

; Moreira, Marina Melo

; Jimenez, Ana Luisa Lodi

; Silva, Lívia Barbosa da

; Thiersch, Laura Maria Silva

; Rodrigues, Carolina Malaquias

; Torres, Bruna Ribeiro

; Costa, Juliana Goulart Dias da

; Diniz, Lilian Martins Oliveira

Revista do Instituto de Medicina Tropical de São Paulo

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2024, Volume 66 elocation e16

Abstract: En | Text: En | PDF: En

ABSTRACT Rocky Mountain Spotted Fever is a rickettsial disease caused by the bacteria Rickettsia rickettsii. In Brazil, the disease is known as Brazilian spotted fever (BSF), being the most significant tick-borne disease in the country. Among the affected patients, only 5% of cases occur in children aged one to nine years. Typical symptoms of the disease are fever, rash, headache and digestive symptoms. Neurological manifestations such as seizures, aphasia and hemiparesis have been described in few patients. This study aimed to describe the case of an infant diagnosed with BSF who presented severe signs of neurological manifestation. rickettsii Brazil BSF, , (BSF) tickborne tick borne country patients 5 years rash seizures manifestation (BSF

https://doi.org/10.1590/s1678-9946202466016

15.

The high biodiversity of benthic organisms in a coastal ecosystem revealed by an integrative approach
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Amaral, A. Cecília Z.

; Cunha, Beatriz P.

; Checon, Helio H.

; Godoy, Angélica S. de

; Silva, Camila F. da

; Corte, Guilherme N.

; Nogueira, João M. de M.

; Fukuda, Marcelo V.

; Steiner, Tatiana M.

; Kawauchi, Gisele Y.

; Turra, Alexander

; Denadai, Márcia R.

; Ferreira, Luciane

; Mendonça, Joel B. de

; Tavares, Marcos

; Leite, Fosca P. P.

; Costa, Mariana F. B.

; Siqueira, Silvana G. L.

; Vieira, Leandro M.

; Dias, Gustavo M.

; Teixeira, Joyce A.

; Rocha, Rosana M.

; Gusmão, Luciana C.

; Borges, Michela

; Alitto, Renata

; Machado, Fabrizio M.

; Passos, Flávio D.

; Cunha, Carlo M.

; Simone, Luiz R.L.

; Araujo, Ana Paula G.

; Carbayo, Fernando

; Bahia, Juliana

; Bulnes, Verónica N.

; Castello-Branco, Cristiana

; Hajdu, Eduardo

; Vilas-Boas, Ana Carolina

; Garraffoni, André R. S.

; Schockaert, Ernest

; Fonseca, Gustavo

; Domenico, Maikon Di

; Curini-Galletti, Marco

; Sørensen, Martin V.

; Hochberg, Rick

; Oliveira, Ana Julia F. C. de

; Zampieri, Bruna Del B.

; Chinelatto, Roberta M.

; Migotto, Alvaro E.

Biota Neotropica

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2024, Volume 24 N. 2 elocation e20231583

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Increasing habitat modification and species loss demand consistent efforts to describe and understand biodiversity patterns. The BIOTA/FAPESP Program was created in this context and it has been a successful initiative to promote studies on biodiversity and conservation in Brazil. The BIOTA/Araçá is an interdisciplinary project that provided a detailed evaluation of the biodiversity of Araçá Bay, a coastal seascape located on the North coast of the state of São Paulo, Southeast Brazil. The bay encompasses multiple habitats, such as beaches, mangroves, rocky shores, and a tidal flat, and provides important ecosystem services. Unfortunately, the bay is the subject of complex social-environmental conflicts that oppose economic, social, and environmental demands (i.e., the expansion of neighboring harbor activities vs. small-scale artisanal fisheries and protection of biodiversity). The present study presents a survey of the benthic species occurring in the different habitats of Araçá Bay, including data obtained during the BIOTA/Araçá project and previous assessments of the area. The benthic species play an important role in marine environments and studying the diversity of these organisms that live associated with the bottom is indispensable for comprehending the environment’s functioning. The macrofauna, meiofauna, and microorganisms associated with soft and hard bottom were listed, and additional information, such as the habitat and geographical distribution, were provided for each species. The checklist includes 826 species, almost 70% recorded during the BIOTA/Araçá project. The most speciose taxa were the annelids (225 spp.), mollusks (194 spp.), and crustaceans (177 spp.). Seven benthic species are endemic to Araçá Bay, 14 are considered threatened, and seven are economically exploited. Furthermore, the bay is the type locality of many taxa, and 11 new benthic species were described based on specimens sampled during the project. This project shows the importance of Araçá Bay as a unique biologically rich environment and highlights the need for conservation efforts in light of the current threats. patterns BIOTAFAPESP BIOTA FAPESP Brazil BIOTAAraçá Paulo beaches mangroves shores flat services Unfortunately socialenvironmental social economic i.e., ie i e (i.e. vs smallscale small scale biodiversity. . biodiversity) area s functioning macrofauna meiofauna listed information distribution 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. 1 threatened exploited Furthermore threats i.e. (i.e 8 7 22 (2 19 (1 17 i.e 2 (

Resumo O aumento da modificação dos habitats e da perda de espécies demanda esforços consistentes para descrever e compreender os padrões de biodiversidade. O programa BIOTA/FAPESP foi criado nesse contexto e é uma iniciativa de sucesso para promover estudos em biodiversidade e conservação no Brasil. O BIOTA/Araçá é um projeto interdisciplinar que promoveu uma avaliação detalhada da biodiversidade da Baía do Araçá, um ecossistema costeiro localizado ao Norte do estado de São Paulo, Sudeste do Brasil. A baía engloba múltiplos habitats, tais como praias, manguezais, costões rochosos, e uma planície de maré, e também fornece importantes serviços ecossistêmicos. Infelizmente, a baía está sujeita à conflitos sócio-ambientais complexos que contrastam demandas econômicas, sociais e ambientais (i.e. a expansão das atividades do porto vizinho vs. a pesca artesanal de pequena escala e a proteção da biodiversidade). O presente estudo apresenta um levantamento das espécies bentônicas que ocorrem nos diferentes habitats da Baía do Araçá, incluindo dados obtidos durante o projeto BIOTA/Araçá e de investigações realizadas anteriormente na área. As espécies bentônicas desempenham um papel importante no ambiente marinho, e estudar a diversidade desses organismos que vivem associados ao fundo é indispensável para compreender o funcionamento do meio ambiente. A macrofauna, meiofauna, e microorganismos associados aos fundos consolidado e inconsolidado foram listados, e informações adicionais foram fornecidas para cada espécie, tais como a distribuição geográfica e nos habitats. O checklist inclui 826 espécies, quase 70% registradas durante o projeto BIOTA/Araçá. Os taxa mais especiosos foram os anelídeos (225 spp.), moluscos (194 spp.), e crustáceos (177 spp.). Entre as espécies bentônicas listadas, sete são endêmicas da Baía do Araçá, 14 são consideradas ameaçadas de extinção, e sete são exploradas economicamente. A baía é a localidade tipo de vários taxa, e 11 novas espécies bentônicas foram descritas com base em espécimes amostrados durante o projeto. Este projeto mostra a importância da Baía do Araçá como um ambiente de riqueza biológica única e demonstra a necessidade de esforços para a sua conservação considerando as atuais ameaças. BIOTAFAPESP BIOTA FAPESP Brasil BIOTAAraçá Paulo praias manguezais rochosos maré ecossistêmicos Infelizmente sócioambientais sócio econômicas i.e. ie i (i.e vs . biodiversidade) área marinho macrofauna meiofauna listados espécie 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. listadas 1 extinção economicamente ameaças i.e 8 7 22 (2 19 (1 17 2 (

https://doi.org/10.1590/1676-0611-bn-2023-1583

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