The pequi pericarp corresponds to the largest portion of the fruit and has a high nutritional value, but it is discarded as an agro-industrial residue. The present study aimed to prepare and characterize flours from the pequi pericarp in terms of their proximate composition, its antioxidant potential before and after the in vitro digestibility process and chemical profile, aiming at the full use of this fruit. The samples of pequi pericarp flours from the cities of Sete Lagoas, Paraopeba and Felixlândia were analyzed. The profile of chemical compounds present in the flours was determined using paper spray mass spectrometry. The in vitro simulated digestion technique was used to verify the stability of the phenolic compounds and the maintenance of the antioxidant capacity of the samples. The flours from the pequi pericarp showed to have higher levels of protein, ash and dietary fiber, compared to the data described in the literature for the pulp of the fruit. The analysis of paper spray mass spectrometry allowed the identification of 46 chemical compounds including amino acids, sugars, organic acids and phenolic compounds. The analysis of the main components showed that there was no chemical variation among the fruits from the cities studied. Through the in vitro digestibility technique, it was possible to observe an increase in the bioaccessibility of phenolic compounds, contributing to increase the already significant antioxidant capacity of the samples. It was concluded that the pequi pericarp flour has the potential to be used as a food ingredient due to the high bioaccessibility of its bioactive compounds, capable of reducing the risk of developing diseases caused by oxidative stress.

Abstract The use of creep feeding for preweaning piglets is important to improve the performance of the piglets. The objective of this experiment was evaluate the effect of using or altering the position of piglet’s creep feeder during lactation on piglet’s performance and on behavior of piglets and sows kept in a hot climate environment. Forty-five sows and their litters at 10 days of lactation were randomly distributed into three treatments: front feeder (FF) - near the side of the sow’s head; back feeder (BF) - near the side of the rump of the sow; and no feeder (NF). All piglets were weighed individually to evaluate the average weight, weight gain and coefficient of variation of the weight. Behavior assessments of the piglets and sows were recorded in 3 period. At 15 and 21 d, piglets of the FF treatment were heavier (P ≤ 0.0001) than piglets of the other treatments. At 10-21d piglets of FF treatment had 76.2% less belly nosing behavior than the NF piglets (P=0.015). The treatments had no impact on behavior of the sows. The creep feeders positioned in the front of the farrowing crate increased piglet growth rate and decreased frequency of belly nosing behavior.

The objective of this work was to determine the chemical composition, total phenolic compounds content, antioxidant capacity through the ABTS (2,20-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) method, as well as to evaluate the use of paper spray ionization mass spectrometry (PS-MS) to obtain fingerprints in different brands of mixed industrialized beverage based on fruits and vegetables with “detox” denomination. The carbohydrate contents ranged from 4.17% (sample D) to 12.72% (sample G). The differences may be related to the composition and proportion of the ingredients present in each formulation. The contents of total phenolic compounds varied between 26.75 mg GAE (gallic acid equivalents) g-1 (sample F) to 48.61 mg GAE g-1 (sample D). The samples A, B and C contain cabbage, spinach and parsley and presented significantly higher phenolic compounds content than the other samples. The antioxidant activity values varied between 1.76 µmol L-1 Trolox g-1 (sample B) to 18.95 µmol L-1 Trolox g-1 (sample E). The brands E, F, and G, that presented higher antioxidant potential, have ingredients pineapple, apple, cabbage, and ginger as common. The analysis of PS-MS allowed for the identification of a number of substances, including organic acids, sugars, and phenolic acids. PS-MS is a relatively simple method, fast and efficient to obtain fingerprints of mixed beverages.

A novel, fast, low-cost and scalable methodology to prepare stable magnetoliposomes (MGLs), without the use of organic solvents, is described. The concept of the work is based on the dual use of soy lecithin associated to a new liposome preparation methodology. Soy lecithin was used to coat the nanoparticles of magnetite (Fe3 O4 @lecithin) and for encapsulation of Fe3 O4 @lecithin (Lip-Fe3 O4 @lecithin). Liposomes with size less than 160 nm, polydispersity index of 0.25 and zeta potential of -41 mV, were prepared with the use of autoclave and sonication. The liposomal formulations containing magnetite and stigmasterol (Lip-Fe3 O4 @lecithin, Lip-Stigma and Lip-Stigma-Fe3 O4 @lecithin) were shown to be promising for the application as antibacterial. The liposomal formulation and magnetite were characterized by the following techniques: conventional and high-resolution transmission electron microscopy (TEM/HRTEM), energy-filtered transmission electron microscopy (EFTEM), proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRPD), dynamic light scattering (DLS) and zeta potential. The Lip-Fe3 O4 @lecithin had a minimum inhibitory concentration (MIC) of 8.4 µg mL-1 in the presence of 200 Oe magnetic field against S. aureus.

The phytochemical study of hexane extract from leaves of Salacia crassifolia resulted in the isolation of 3β-palmitoxy-urs-12-ene, 3-oxofriedelane, 3β-hydroxyfriedelane, 3-oxo-28-hydroxyfriedelane, 3-oxo-29-hydroxyfriedelane, 28,29-dihydroxyfriedelan-3-one, 3,4-seco-friedelan-3-oic acid, 3β-hydroxy-olean-9(11):12-diene and the mixture of α-amirin and β-amirin. β-sitosterol, the polymer gutta-percha, squalene and eicosanoic acid were also isolated. The chemical structures of these constituents were established by IR, 1H and 13C NMR spectral data. Crude extracts and the triterpenes were tested against Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis and no activity was observed under the in vitro assay conditions. The hexane, chloroform, ethyl acetate and ethanol crude extracts, and the constituent 3,4-seco-friedelan-3-oic acid and 28,29-dihydroxyfriedelan-3-one showed in vitro antimicrobial activity against Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Streptococcus sanguinis and Candida albicans.

Em 1991, foi realizado um estudo de prevalência sobre o hábito de fumar entre 864 alunos da 6ª à 8ª série do 1º grau de oito escolas municipais de Sapiranga, Rio Grande do Sul. A coleta de dados foi feita através de um questionário aplicado e recolhido por professoras treinadas, em sala de aula. Entre os escolares, 3,2% eram fumantes, embora 20,3% tenham sido expostos pelo menos uma vez ao tabaco. A idade média de início do hábito foi de 14,7 anos no sexo masculino e 15,0 no sexo feminino. A prevalência mostrou uma tendência a aumentar junto com a idade e com o ano escolar. Quanto ao sexo, o número de fumantes foi significativamente maior no sexo masculino (p < 0,05). O hábito de fumar do irmão mais velho e do pai parece reforçar a adesão do escolar ao mesmo.

A prevalence study on smoking habits was carried out in 1991, among 864 school children from eight municipal schools in Sapiranga, in the State of Rio Grande do Sul, Brazil, from the 6th through 8th grade of primary school. Data was collected in class, using a questionnaire applyed by trained teachers. Among students, 3.2% were smokers, although 20.3% had been exposed to tobacco. Males had begun the smoking habit at a mean age of 14.7 years and females at about 15 years old. The prevalence showed an increasing trend by age and individual school grade. As for gender, male smokers outnumbered females (p < 0,05). Smoking by older brothers and fathers seems to be positively associated with acquisition of the habit by school children.

Em 1991, foi realizado um estudo de prevalência sobre o hábito de fumar entre 864 alunos da 6ª à 8ª série do 1º grau de oito escolas municipais de Sapiranga, Rio Grande do Sul. A coleta de dados foi feita através de um questionário aplicado e recolhido por professoras treinadas, em sala de aula. Entre os escolares, 3,2% eram fumantes, embora 20,3% tenham sido expostos pelo menos uma vez ao tabaco. A idade média de início do hábito foi de 14,7 anos no sexo masculino e 15,0 no sexo feminino. A prevalência mostrou uma tendência a aumentar junto com a idade e com o ano escolar. Quanto ao sexo, o número de fumantes foi significativamente maior no sexo masculino (p < 0,05). O hábito de fumar do irmão mais velho e do pai parece reforçar a adesão do escolar ao mesmo.

A prevalence study on smoking habits was carried out in 1991, among 864 school children from eight municipal schools in Sapiranga, in the State of Rio Grande do Sul, Brazil, from the 6th through 8th grade of primary school. Data was collected in class, using a questionnaire applyed by trained teachers. Among students, 3.2% were smokers, although 20.3% had been exposed to tobacco. Males had begun the smoking habit at a mean age of 14.7 years and females at about 15 years old. The prevalence showed an increasing trend by age and individual school grade. As for gender, male smokers outnumbered females (p < 0,05). Smoking by older brothers and fathers seems to be positively associated with acquisition of the habit by school children.