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IL-1β and IL-17 in cutaneous lupus erythematous skin biopsies: could immunohistochemicals indicate a tendency towards systemic involvement?
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Lovato, Barbara Hartung

; Fogagnolo, Leticia

; Souza, Elemir Macedo de

; Silva, Larissa Juliana Batista da

; Velho, Paulo Eduardo Neves Ferreira

; Cintra, Maria Leticia

; Teixeira, Fernanda

Anais Brasileiros de Dermatologia

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jan 2024, Volume 99 N. 1 Pages 66 - 71

Abstract: En | Text: En | PDF: En

Abstract Background: Only a fraction of patients with cutaneous lupus erythematosus (CLE) will eventually progress toward systemic disease (SLE). Objective: To find inflammatory biomarkers which could predict the progression of cutaneous lupus erythematosus (CLE) into systemic lupus erythematosus (SLE) using immunohistochemical (IHC) assays. Methods: Immunohistochemical markers for cytotoxic, inflammatory, and anti-inflammatory responses and morphometric methods were applied to routine paraffin sections of skin biopsies, taken from lesions of 59 patients with discoid lupus, subacute lupus, and lupus tumidus. For the diagnosis of SLE, patients were classified by both the American College of Rheumatology (ACR-82) and the Systemic Lupus International Collaborating Clinics (SLICC-12) systems. Results: Skin samples from CLE/SLE +patients presented higher expression of IL-1β (ARC-82: p = 0.024; SLICC-12: p = 0.0143) and a significantly higher number of cells marked with granzyme B and perforin (ARC: p = 0.0097; SLICC-12: p = 0.0148). Biopsies from CLE/SLE- individuals had higher expression of IL-17 (ARC-82: p = 0.0003; SLICC-12: p = 0.0351) and presented a positive correlation between the density of granzyme A+and FoxP3+ cells (ARC-82: p = 0.0257; SLICC-12: p = 0.0285) and CD8+ cells (ARC-82: p = 0.0075; SLICC-12: p = 0.0102), as well as between granulysin-positive and CD8+ cells (ARC-82: p = 0.0024; SLICC-12: p = 0.0116). Study limitations: Patients were evaluated at a specific point in their evolution and according to the presence or not of systemic disease. The authors cannot predict how many more, from each group, would have evolved towards SLE in the following years. Conclusions: In this cohort, immunohistochemical findings suggested that patients with a tendency to systemic disease will show strong reactivity for IL-1β, while those with purely cutaneous involvement will tend to express IL-17 more intensely.

https://doi.org/10.1016/j.abd.2023.02.007

Implementação do Projeto ApiceOn: Desenvolvimento e Prática do Enfermeiro
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Ibiapino, Juliana Louise da Silva

; Chirelli, Mara Quaglio

; Rezende, Kátia Terezinha Alves

; Marin, Maria José Sanches

; Mazzetto, Fernanda Moerbeck Cardoso

; Mielo, Márcio

New Trends in Qualitative Research

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oct 2023, Volume 18 elocation e886

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução: O Projeto de Aprimoramento e Inovação no Cuidado e Ensino em Obstetrícia e Neonatologia (ApiceOn), propõe integrar os hospitais de ensino à Rede Cegonha, visa as áreas que se referem ao parto e nascimento, planejamento reprodutivo pós-parto e pós aborto, atenção as mulheres que estão em situação de violência sexual e abortamento e aborto legal, por meio de práticas humanizadas e evidências científicas com a inserção de enfermeiros obstetras e obstetrizes, diretamente relacionadas ao aprendizado dos futuros profissionais. Objetivo: analisar o processo de implementação do projeto ApiceOn em uma instituição hospitalar e como se desenvolveu a inserção do enfermeiro e do enfermeiro obstetra. Métodos: pesquisa de abordagem qualitativa, realizada por meio de entrevitas semi-estruturadas com 15 enfermeiros e tratamento dos dados pela Análise Temática de Conteúdo. Identificou-se como tema o conhecimento e desafios do Projeto ApiceOn: do significado, da implementação, do desenvolvimento e da prática do enfermeiro. Resultados: considera-se que as entrevistadas possuem conhecimento incipiente sobre o projeto, sua implementação e desenvolvimento não há inserção dos enfermeiros e enfermeiros obstetras frente ao projeto. O processo de implantação e implementação do Projeto ApiceOn requer a constituição de cogestão, visto que necessita da construção de novas práticas ancoradas no modelo de vigilância à saúde, superando o modelo biomédico, para tanto a lógica de formação dos profissionais está pautada na educação permanente. Conclusão: há a necessidade de mudança do paradigma de cuidado em saúde e, consequentemente, do processo de trabalho com as gestantes/parturientes/famílias, por meio da implementação e formação para o desenvolvimento do projeto ApiceOn, utilizando protocolos para atuação dos profissionais e organização estrutural.

Abstract Introduction: The Project for Improvement and Innovation in Care and Teaching in Obstetrics and Neonatology (ApiceOn) proposes to integrate teaching hospitals to Rede Cegonha, targets areas related to labor and birth, postpartum and post-abortion reproductive planning, care for women who are in a situation of sexual violence and abortion and legal abortion, through humanized practices and scientific evidence with the insertion of obstetric nurses and midwives, directly related to the learning of future professionals. Goals: analyze the ApiceOn project implementation process in a hospital institution and how the insertion of the nurse and the midwife. Methods: qualitative approach research, with 15 nurses through semi-structured interviews and data processing by Analysis thematic content. The knowledge and challenges of the ApiceOn Project were identified as a theme: the meaning, implementation, development and practice of nursing. Results: it is considered that the interviewees have incipient knowledge about the project, its implementation and development and there is no insertion of nurses and midwives in front of the project. The implementation process of the ApiceOn Project requires the constitution of co-management, since it requires the construction of new practices anchored in the health surveillance model, surpassing the biomedical model, therefore, the training logic of professionals is based on permanent education. Conclusions: there is a need to change the paradigm of health care and, consequently, the work process with pregnant women/pregnant women/families, through the implementation and training for the development of the ApiceOn project, using protocols for the performance of professionals and structural organization.

https://doi.org/10.36367/ntqr.18.2023.e886

Risco e rabisco: representações sociais de crianças expressas em desenhos acerca do procedimento de coleta sanguínea
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Lucas, Eduardo Alexander Júlio César Fonseca

; Rocha, Tatiana da Silva Mello

; Santos, Antonio Eduardo Vieira dos

; Lima, Juliana Dias Vieira

; Souza, Samhira Vieira Franco de

; Lemos, Natália Rodrigues Pontes

; Telles, Alexandre Oliveira

; Lucas, Simone Fonseca

; Evaristo, Fernanda Fonseca Lucas

Ciência & Saúde Coletiva

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apr 2023, Volume 28 N. 4 Pages 1031 - 1042

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract The scope of this article was to identify the social representations of children in a municipal public school in Rio de Janeiro, with respect to the blood sampling procedure and to discuss the social representations that emerge, in the light of the performance of the family health team in carrying out this procedure. Moscovisci’s method/theory of analysis of social representations based on a qualitative approach was used. For data collection, the projective technique of the esthetic production scene and the student’s speech was used. The results were grouped into four categories: 1) structural dimensions involved in the blood sampling procedure; 2) dimension of the human component and its interface in the blood sampling procedure; 3) the psycho-emotional dimension portrayed in the blood sampling procedure; and 4) blood sampling: the importance for student health. With respect to the importance of blood sampling, we highlight the perception of students regarding the importance of actions aimed at prevention and health care promotion.

Resumo Objetivou-se identificar as representações sociais de crianças de uma escola pública municipal do Rio de Janeiro acerca do procedimento de coleta sanguínea e discutir as representações sociais emergidas, à luz da atuação da equipe de saúde da família na realização desse procedimento. Utilizou-se o método e a teoria de análise das representações sociais fundamentadas por Moscovisci e Jodelet, numa abordagem qualitativa. Para a coleta de dados, empregou-se a técnica projetiva de cena de produção estética e do discurso do escolar. Os resultados foram agrupados em quatro categorias: 1) dimensões estruturais que envolvem o procedimento de coleta sanguínea; 2) dimensão do componente humano e sua interface no procedimento de coleta sanguínea; 3) a dimensão psicoemocional retratada no procedimento de coleta sanguínea e 4) a coleta de sangue: a importância para a saúde do escolar. No que diz respeito à importância da coleta sanguínea, evidenciamos a percepção de escolares para a importância de ações voltadas à prevenção e promoção da saúde.

https://doi.org/10.1590/1413-81232023284.00552022

Annual litterfall in an Atlantic Forest fragment in Northeast Brazil
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da Silva Barreto, Wanctuy

; Teixeira das Chagas, Kyvia Pontes

; da Silva Santana, José Augusto

; de Oliveira Ribeiro, Ciro

; de Melo Almeida, Débora

; Fonseca Lucas, Fernanda Moura

; Lorensi do Canto, Juliana

Idesia (Arica)

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mar 2023, Volume 41 N. 1 Pages 97 - 104

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The ecological structure of forest fragments is closely related to the dynamics of biological flows directly dependent on the decomposition of the litterfall, which is the main mechanism of recycling nutrients to plants. In this sense, the objective of the present work was to quantify the annual litterfall productionduring the period from April 2013 to March 2014 in Mata do Bebo, an Atlantic Forest fragment located in Macaíba, Rio Grande do Norte. 10 conical collectors were used to obtain the data on the litter contribution, with an average opening area of 0.128 m2, placed 1 m above the ground. The material was collected monthly, dried at air temperature for 48 h, and separated into four fractions: leaves, bark and branches, reproductive material; and miscellaneous material. After separation, the fractions were weighed and stored. The total contribution of litter was uninterrupted and totaled 5,038.72 kg ha-1 yea-1, with the highest production in September, at the beginning of the dry season, and the lowest in July. The foliar fraction made the largest contribution, with 3,744.26 kg ha-1 yean1, followed by the reproductive material, which reached 846.29 kg ha-1 year-1, branches and bark, with 396.10 kg ha-1 year-1, and miscellaneous material, with 52,069 kg ha-1 year-1. The correlations of litterfall production with rainfall and temperature were negative. The litterfall production in the fragment was lower than that obtained in other remaining Atlantic Forest areas, possibly because it is located in a region considered an ecotone between the Caatinga and Atlantic Forest biomes, besides the anthropic effect to which it has been submitted for decades.

RESUMEN La estructura ecológica de un fragmento de bosque está estrechamente relacionada con la dinámica de los flujos biológicos, ya que depende directamente de la descomposición de la hojarasca, que representa el principal mecanismo de disponibilidad y retorno de nutrientes a las plantas. El objetivo de este trabajo fue cuantificar la producción anual de hojarasca en un fragmento de Bosque Atlántico en el noreste de Brasil. Para obtener datos sobre la producción de hojarasca se utilizaron 10 colectores cónicos con una superficie media de 0,128 m 2 , situados a 1 m de altura. El material se recogía mensualmentg, se secaba a temperatura ambiente durante 48 h y se separaba en fracciones: hojas, corteza, ramas, material de reproducción y miscelánea. Después de la separación las fracciones fueron pesadas y almacenadas. La producción de hojarasca fue ininterrumpida y ascendió a un total de 5.038,72 kg ha -1 .año~ 1 , con la mayor producción en septiembre, al comienzo del período seco, y la menor en julio. La fracción foliar fue la que presentó el mayor aporte en la producción de la hojarasca, con 3.744,26 kg ha -1 año- 1 , seguida del material de reproducción que alcanzó a 846,29 kg ha -1 año -1 , las ramas y las cáscaras con 396,10 kg ha -1 añoy material misceláneo con 52.069 kg ha -1 año -1 . Las correlaciones de la producción de hojarasca con la lluvia y la temperatura resultaron negativas. La producción de hojarasca en el fragmento fue menor que la obtenida en otras zonas del Bosque Atlántico, posiblemente porque se encuentra en una región considerada un ecotono con la Caatinga, además del efecto antrópico al que ha estado sometido durante décadas.

Hereditary hemochromatosis beyond hyperferritinemia: Clinical and laboratory investigation of the patient’s profile submitted to phlebotomy in two reference centers in southern Brazil
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Kersting, Nathalia

; Fontana, Juliana Cristine

; Athayde, Fabiane Pohlmann de

; Carlotto, Fernanda Marcante

; Machado, Bruna Accorsi

; Araújo, Cristiane da Silva Rodrigues de

; Sekine, Leo

; Onsten, Tor Gunnar Hugo

; Leistner-Segal, Sandra

Genetics and Molecular Biology

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jan 2023, Volume 46 N. 2 elocation e20220230

Abstract: En | Text: En | PDF: En

Abstract Hereditary Hemochromatosis is a disorder characterized by iron deposition in several organs and hyperferritinemia. The most studied variants are linked to the HFE gene. In Brazil, surveys that characterize this population are scarce, with no sampling in the state of Rio Grande do Sul. Our objective is to carry out a data collection focusing on the profile of this population and the influence of the most frequently HFE variants. Two centers were enrolled: Hospital de Clínicas de Porto Alegre and Hospital São Vicente de Paulo. Patients with hyperferritinemia and undergoing phlebotomy were invited. Clinical data were collected, including HFE investigation. Among the descriptive data, the allele frequency of the C282Y variant (0.252) stands out, which differs from the national scenario. Systemic arterial hypertension was the most cited comorbidity. Differences between centers were observed, highlighting higher frequency of H63D cases in HSVP (p<0.01). Genotypes were stratified according to deleterious effect of C282Y variant. Higher transferrin saturation and number of phlebotomies were observed in the C282Y/C282Y cases (p<0.001). Positive family history for hyperferritinemia was more prevalent in compound heterozygotes (p<0.01). The results presented confirm the importance of encouraging such studies and reiterate the need for greater attention to this population.

https://doi.org/10.1590/1678-4685-gmb-2022-0230

Risco e rabisco: representações sociais de crianças expressas em desenhos acerca do procedimento de coleta sanguínea
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Lucas, Eduardo Alexander Júlio César Fonseca

; Rocha, Tatiana da Silva Mello

; Santos, Antonio Eduardo Vieira dos

; Lima, Juliana Dias Vieira

; Souza, Samhira Vieira Franco de

; Lemos, Natália Rodrigues Pontes

; Telles, Alexandre Oliveira

; Lucas, Simone Fonseca

; Evaristo, Fernanda Fonseca Lucas

Ciência & Saúde Coletiva

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2023, Volume 28 N. 4 Pages 1031 - 1042

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1413-81232023284.00552022

Differences in determinants of active aging between older Brazilian and English adults: ELSI-Brazil and ELSA
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Silva, Janderson Diego Pimenta da

; Martins, Isadora Viegas

; Braga, Luciana Helena Reis

; Oliveira, Cesar Messias de

; Lima-Costa, Maria Fernanda

; Braga, Luciana de Souza

; Torres, Juliana Lustosa

Cadernos de Saúde Pública

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2023, Volume 39 N. 9 elocation e00076823

Abstract: Pt Es En | Text: Pt Es En | PDF: Pt | PDF: Es | PDF: En

Resumo: Este estudo transversal objetivou investigar as diferenças nos determinantes do envelhecimento ativo entre idosos brasileiros e ingleses, e verificar a associação de determinantes comportamentais, pessoais e sociais com a saúde física. A pesquisa baseou-se nas coortes ELSI-Brasil (2015-2016) e ELSA (2016-2017). Os determinantes do envelhecimento ativo incluíram os determinantes comportamentais (tabagismo, sedentarismo e má qualidade do sono), pessoais (função cognitiva e satisfação com a vida) e sociais (educação, solidão e voluntariado), de acordo com a Organização Mundial da Saúde. A saúde física incluiu limitação de atividades e multimorbidade. Prevalências ajustadas por idade e sexo foram calculadas para cada indicador e escores médios, utilizando-se a regressão binomial negativa para a análise estatística. A pesquisa incluiu 16.642 participantes, sendo 9.409 do Brasil e 7.233 da Inglaterra. No geral, todos os determinantes do envelhecimento ativo foram piores no Brasil do que na Inglaterra, exceto a satisfação com a vida (sem diferença). A diferença mais marcante refere-se ao escore de determinantes sociais no Brasil (diferença média de 0,18; p < 0,05), principalmente devido à escolaridade significativamente menor no Brasil (70,6%; intervalo de 95% de confiança - IC95%: 69,7-71,5) do que na Inglaterra (37,1%; IC95%: 35,1-39,1). Todos os determinantes (comportamentais, pessoais e sociais) estiveram associados à saúde no Brasil e na Inglaterra. No entanto, o domínio comportamental foi mais fortemente associado à saúde na Inglaterra (coeficiente = 2,76; IC95%: 2,46-3,10) do que no Brasil (coeficiente = 1,38; IC95%: 1,26-1,50) (p < 0,001). Idosos ingleses se beneficiam mais de comportamentos mais saudáveis do que os brasileiros, que dependem mais de políticas sociais.

Resumen: Este estudio transversal tuvo como objetivo investigar las diferencias en los determinantes del envejecimiento activo entre personas mayores brasileñas e inglesas, y verificar la asociación de determinantes conductuales, personales y sociales con la salud física. La investigación se basó en las cohortes ELSI-Brasil (2015-2016) y ELSA (2016-2017). Los determinantes del envejecimiento activo incluyeron determinantes conductuales (tabaquismo, sedentarismo y mala calidad del sueño), personales (función cognitiva y satisfacción con la vida) y sociales (educación, soledad y voluntariado), según la Organización Mundial de la Salud. La salud física incluyó la limitación de actividades y la multimorbilidad. Se calcularon las prevalencias ajustadas por edad y sexo para cada indicador y los puntajes medios, usando la regresión binomial negativa para el análisis estadístico. La encuesta incluyó a 16.642 participantes, 9.409 de Brasil y 7.233 de Inglaterra. En general, todos los determinantes del envejecimiento activo fueron peores en Brasil que en Inglaterra, salvo la satisfacción con la vida (sin diferencia). La diferencia más llamativa se refiere al puntaje de los determinantes sociales en Brasil (diferencia media de 0,18; p < 0,05), sobre todo debido al nivel educativo significativamente más bajo en Brasil (70,6%; intervalo de 95% de confianza - IC95%: 69,7-71,5) que en Inglaterra (37,1%; IC95%: 35,1-39,1). Todos los determinantes (conductuales, personales y sociales) se asociaron con la salud en Brasil y en Inglaterra. Sin embargo, el dominio conductual se asoció más fuertemente con la salud en Inglaterra (coeficiente = 2,76; IC 95% 2,46-3,10) que en Brasil (coeficiente = 1,38; IC95%: 1,26-1,50) (p < 0,001). Las personas mayores inglesas se benefician más de comportamientos más saludables que los brasileños, que dependen más de las políticas sociales.

Abstract: This study aimed to investigate differences in determinants of active aging between older Brazilian and English adults and to verify the association of behavioral, personal, and social determinants with physical health. This cross-sectional study was based on the ELSI-Brazil (2015-2016) and ELSA (2016-2017) cohorts. Active aging determinants included behavior (smoking, sedentary lifestyle, and poor sleep quality), personal (cognitive function and life satisfaction), and social determinants (education, loneliness, and volunteering), according to the World Health Organization. Physical health included activities limitation and multimorbidity. We estimated age- and sex-adjusted prevalence for each indicator and mean score, and used the negative binomial regression for statistical analysis. We included 16,642 participants, 9,409 from Brazil and 7,233 from England. Overall, all active aging determinants were worse in Brazil than in England, except for life satisfaction (no difference). The most remarkable difference was found for social determinants score in Brazil (mean difference of 0.18; p < 0.05), mainly due to a significantly lower education level in Brazil (70.6%; 95% confidence interval - 95%CI: 69.7-71.5) than England (37.1%; 95%CI: 35.1-39.1). All determinants (behavioral, personal, and social) were associated with health in Brazil and in England. However, the behavioral domain was stronger associated with health in England (coefficient = 2.76; 95%CI: 2.46-3.10) than in Brazil (coefficient = 1.38; 95%CI: 1.26-1.50; p < 0.001). Older English adults beneficiate more from healthier behaviors than Brazilians, which depend more on social policies.

https://doi.org/10.1590/0102-311xen076823

How many species of Mollusca are there in Brazil? A collective taxonomic effort to reveal this still unknown diversity
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Machado, Fabrizio M.

; Miranda, Marcel S.

; Salvador, Rodrigo B.

; Pimenta, Alexandre D.

; Côrtes, Mariana O.

; Gomes, Jaime A.J.

; Miyahira, Igor C.

; Agudo-Padrón, Ignacio

; Oliveira, Cléo D.C.

; Caetano, Carlos H.S.

; Coelho, Paulo R.S.

; D’Ávila, Sthefane

; Arruda, Eliane P. de

; Almeida, Sérgio M. de

; Gomes, Suzete R.

; Alvim, Juliana

; Galvão-Filho, Hilton

; L. Ferreira-Júnior, Augusto

; Marques, Rodrigo C.

; Martins, Inês

; Souza, Leonardo S. de

; Arruda, Janine O.

; Cavallari, Daniel C.

; Santos, Sonia B. dos

; Pedro, Natan C.

; Salles, Anna C. de A.

; Dornellas, Ana P.S.

; Lima, Tarcilla C. de

; Amaral, Vanessa S. do

; Silva, Fernanda S.

; Passos, Flávio D.

; Thiengo, Silvana S.

; Leite, Tatiana S.

; Simone, Luiz R.L.

Zoologia (Curitiba)

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2023, Volume 40 elocation e23026

Abstract: En | Text: En | PDF: En

ABSTRACT The expression ‘you need to know to conserve’ is a well-known cliche among biologists. Documenting the richness of a group of organisms is the first step towards understanding biodiversity and preparing efficient conservation plans. In this context, many efforts have been made to quantify the number of species on Earth and estimate the number of species still unknown to science. A few countries have complete and integrated databases estimating the approximate number of species recorded for their territory, particularly in the Global South. In Brazil, a country of continental dimensions, revealing the richness of the second most diverse clade of invertebrates (=Mollusca) has been a goal of taxonomists. Recently, in an unprecedented, collective, and integrated effort among Brazilian malacologists, it was possible to estimate how many valid species of molluscs are there in Brazil. In this effort, more than 30 mollusc experts joined together to update the Taxonomic Catalogue of the Brazilian Fauna (TCBF), a governmental website that allows a quick and real-time updating of all Metazoan. So far, more than 5,000 updates have been made in TCBF, indicating the presence of 3,552 valid species of molluscs in Brazil, distributed among the main clades as follows: Caudofoveata (10 spp.), Solenogastres (6 spp.), Polyplacophora (35 spp.), Scaphopoda (43 spp.), Cephalopoda (92 spp.), Bivalvia (629 spp.) and Gastropoda (2,737 spp.). The present study, in addition to demonstrating for the first time the richness of Brazilian molluscs, also presents the state of the art of this important phylum of invertebrates highlighting its most representative and neglected groups.

https://doi.org/10.1590/s1984-4689.v40.e23026

Cuidados para prevenção de lesão por pressão realizada por enfermeiros em um hospital de ensino
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Silva, Taiara Fonseca da

; Tristão, Fernanda Santana

; Echevarria-Guanilo, Maria Elena

; Zillmer, Juliana Graciela Vestena

; Oliveira, Camila Maria de

; Blair, Isabela Jéssica Queiroz

Revista Uruguaya de Enfermería (En línea)

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2023, Volume 18 N. 2 elocation e205

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo: Objetivo: identificar os fatores de risco para lesão por pressão reconhecidos e os cuidados de enfermagem realizados em relação à prevenção de lesão por pressão. Metodologia: Estudo com abordagem quantitativa, do tipo transversal e descritivo, realizado em unidades de internação adulto de um hospital de ensino. Os dados foram coletados por meio de um questionário autoaplicável. Os dados foram analisados por meio do Programa SPSS versão 22.0, as variáveis foram analisadas por estatística descritiva com o propósito de avaliar associação entre as variáveis categóricas, utilizou-se o teste Qui-Quadrado com nível de 0,05 de significância. Resultado: O estudo envolveu 40 enfermeiros. Quanto aos fatores de risco para lesão por pressão (100 %) dos enfermeiros indicou mobilidade no leito prejudicado, (97,5 %) proeminência óssea aparente. Quanto, a capacitação sobre prevenção de lesão por pressão, (62,5 %) participou de o algum curso de capacitação. A avaliação da condição da pele no momento da admissão foi indicada como um cuidado realizado pela maioria dos enfermeiros (67,5 %) e (22,5 %) dos enfermeiros não levam em conta o tempo de admissão para realizar a avaliação da pele dos pacientes. Entre os cuidados para a prevenção de lesão por pressão realizados pelos enfermeiros destacaram-se a mudança de decúbito e a utilização de coxins (95,0 %). Conclusão: Embora os enfermeiros demonstrem conhecimento a respeito do tema, destaca-se a necessidade do desenvolvimento de protocolos de prevenção de lesão por pressão nos hospitais, assim como investimento em materiais e equipamentos adequados.

Abstract: Objective: to identify the recognized risk factors for pressure injury and the nursing care performed in relation to the prevention of pressure injury. Methodology: Study with a quantitative approach, cross-sectional and descriptive, performed in adult hospitalization units of a teaching hospital. The data were collected through a self-applied questionnaire. The data were analyzed using the SPSS Program version 22.0, the variables were analyzed using descriptive statistics with the purpose of evaluating the association between categorical variables, using the Chi-Square test with a 0.05 significance level. Result: The study involved 40 nurses. Regarding the risk factors for pressure injury, 100 % of the nurses indicated harmed bed mobility, 97.5 % apparent bone prominence. As for training on pressure injury prevention, 62.5 % attended to some training course. The evaluation of the skin condition at the time of admission was indicated as a care performed by most nurses (67.5 %) and 22.5 % of the nurses do not consider admission time to perform skin evaluation of patients. Among the care for the prevention of pressure injury performed by the nurses, the change in decubitus and the use of cushions stood out (95.0 %). Conclusion: Although nurses demonstrate knowledge on the subject, the need to develop pressure injury prevention protocols in hospitals is highlighted, as well as investment in appropriate materials and equipment.

Resumen: Objetivo: Identificar los factores de riesgo reconocidos para lesión por presión y los cuidados de enfermería realizados con relación a la prevención de lesión por presión. Metodología: Estudio con planteamiento cuantitativo del tipo transversal y descriptivo realizado en unidades de hospitalización adulta de un hospital escuela. Se recolectaron los datos por medio de un cuestionario autoaplicable. Se analizaron los datos mediante la versión SPSS 22.0 mientras las variables, por estadística descriptiva con el propósito de evaluar la asociación entre las variables categóricas. Se utilizó la prueba Chi-cuadrado con nivel 0.05 de significancia. Resultado: Cuarenta enfermeros participaron en el estudio. En cuanto a los factores de riesgo para lesión por presión, el 100 % de los enfermeros reportó movilidad perjudicada en la cama y el 97,5 %, prominencia ósea aparente. En cuanto a la capacitación sobre la prevención de lesión por presión, el 62,5 % había participado en algún curso de capacitación. La evaluación de la condición de la piel en el momento de la hospitalización fue reportada como un cuidado realizado por la mayoría de los enfermeros (67,5 %) mientras el 22,5 % no toma en consideración el tiempo de hospitalización para evaluar la piel de los pacientes. Entre los cuidados realizados por los enfermeros para la prevención de lesión por presión se destacaron el cambio de decúbito y la utilización de cojinetes (95,0 %). Conclusión: Aunque los enfermeros demuestren conocimiento acerca del tema, se resalta la necesidad de desarrollar protocolos de prevención de lesión por presión en los hospitales, así como la inversión en materiales y equipos adecuados.

https://doi.org/10.33517/rue2023v18n2a8

10.

Metabolic effect of low fluoride levels in the islets of NOD mice: integrative morphological, immunohistochemical, and proteomic analyses
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TREVIZOL, Juliana Sanches

; DIONIZIO, Aline

; DELGADO, Aislan Quintiliano

; VENTURA, Talita Mendes Oliveira

; RIBEIRO, Caroline Fernanda da Silva

; RIBEIRO, Laura

; BUZALAF, Nathalia Rabelo

; CESTARI, Tânia Mary

; MAGALHÃES, Ana Carolina

; SUZUKI, Maiko

; BOSQUEIRO, José Roberto

; BUZALAF, Marília Afonso Rabelo

Journal of Applied Oral Science

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2023, Volume 31 elocation e20230036

Abstract: En | Text: En | PDF: En

Abstract Fluoride (F) has been widely used to control dental caries, and studies suggest beneficial effects against diabetes when a low dose of F is added to the drinking water (10 mgF/L). Objectives This study evaluated metabolic changes in pancreatic islets of NOD mice exposed to low doses of F and the main pathways altered by the treatment. Methodology In total, 42 female NOD mice were randomly divided into two groups, considering the concentration of F administered in the drinking water for 14 weeks: 0 or 10 mgF/L. After the experimental period, the pancreas was collected for morphological and immunohistochemical analysis, and the islets for proteomic analysis. Results In the morphological and immunohistochemical analysis, no significant differences were found in the percentage of cells labelled for insulin, glucagon, and acetylated histone H3, although the treated group had higher percentages than the control group. Moreover, no significant differences were found for the mean percentages of pancreatic areas occupied by islets and for the pancreatic inflammatory infiltrate between the control and treated groups. Proteomic analysis showed large increases in histones H3 and, to a lesser extent, in histone acetyltransferases, concomitant with a decrease in enzymes involved in the formation of acetyl-CoA, besides many changes in proteins involved in several metabolic pathways, especially energy metabolism. The conjunction analysis of these data showed an attempt by the organism to maintain protein synthesis in the islets, even with the dramatic changes in energy metabolism. Conclusion Our data suggests epigenetic alterations in the islets of NOD mice exposed to F levels comparable to those found in public supply water consumed by humans.

https://doi.org/10.1590/1678-7757-2023-0036

11.

Chikungunya virus infection in the southernmost state of Brazil was characterised by self-limited transmission (2017-2019) and a larger 2021 outbreak
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Gregianini, Tatiana Schäffer

; Salvato, Richard Steiner

; Barcellos, Regina Bones

; Godinho, Fernanda Marques

; Ruivo, Amanda Pellenz

; de Melo, Viviane Horn

; Schroder, Júlio Augusto

; Martiny, Fernanda Letícia

; Möllmann, Erica Bortoli

; Favreto, Cátia

; Baethgen, Ludmila Fiorenzano

; Ferreira, Vithoria Pompermaier

; de Lima, Lívia Eidt

; Piazza, Cláudia Fasolo

; Machado, Taís Raquel Marcon

; Becker, Irina Marieta

; Ramos, Raquel Rocha

; Frölich, Guilherme Carey

; Rossetti, Alana Fraga

; Almeida, Lucas da Cunha

; Rodrigues, Tahiana Machado Antunes

; Bragança, Isabella Tabelli

; Campos, Aline Alves Scarpellini

; Manzoni, Verônica Baú

; Machado, Lais Ceschini

; da Silva, Luisa Maria Inácio

; de Oliveira, André Luiz Sá

; Paiva, Marcelo Henrique Santos

; Nunes, Zenaida Marion Alves

; de Almeida, Paula Rodrigues

; Demoliner, Meriane

; Gularte, Juliana Schons

; da Silva, Mariana Soares

; Filippi, Micheli

; Pereira, Vyctoria Malayhka de Abreu Góes

; Spilki, Fernando Rosado

; da Veiga, Ana Beatriz Gorini

; Wallau, Gabriel Luz

Memórias do Instituto Oswaldo Cruz

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2023, Volume 118 elocation e220259

Abstract: En | Text: En | PDF: En

BACKGROUND Chikungunya is a mosquito-borne virus that has been causing large outbreaks in the Americas since 2014. In Brazil, Asian-Caribbean (AC) and East-Central-South-African (ECSA) genotypes have been detected and lead to large outbreaks in several Brazilian states. In Rio Grande do Sul (RS), the southernmost state of Brazil, the first cases were reported in 2016. OBJECTIVES AND METHODS We employed genome sequencing and epidemiological investigation to characterise the Chikungunya fever (CHIKF) burden in RS between 2017-2021. FINDINGS We detected an increasing CHIKF burden linked to travel associated introductions and communitary transmission of distinct lineages of the ECSA genotype during this period. MAIN CONCLUSIONS Until 2020, CHIKV introductions were most travel associated and transmission was limited. Then, in 2021, the largest outbreak occurred in the state associated with the introduction of a new ECSA lineage. CHIKV outbreaks are likely to occur in the near future due to abundant competent vectors and a susceptible population, exposing more than 11 million inhabitants to an increasing infection risk.

https://doi.org/10.1590/0074-02760220259

12.

Differences in determinants of active aging between older Brazilian and English adults: ELSI-Brazil and ELSA
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Silva, Janderson Diego Pimenta da

; Martins, Isadora Viegas

; Braga, Luciana Helena Reis

; Oliveira, Cesar Messias de

; Lima-Costa, Maria Fernanda

; Braga, Luciana de Souza

; Torres, Juliana Lustosa

Cadernos de Saúde Pública

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2023, Volume 39 N. 9 elocation e00076823

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

https://doi.org/10.1590/0102-311xen076823

13.

Systemic and local inflammatory response after implantation of biomaterial in critical bone injuries
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Brassolatti, Patricia

; Castro, Cynthia Aparecida de

; Santos, Hugo Leonardo dos

; Simões, Isabelle Taira

; Almeida-Lopes, Luciana

; Silva, Juliana Virginio da

; Duarte, Fernanda Oliveira

; Luna, Genoveva Lourdes Flores

; Beck, Wladimir Rafael

; Bossini, Paulo Sergio

; Anibal, Fernanda de Freitas

Acta Cirúrgica Brasileira

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2023, Volume 38 elocation e383823

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: To evaluate inflammatory response in critical bone injuries after implantation of the biomaterial composed of hydroxyapatite (HA)/poly (lactic-coglycolic acid) (PLGA)/BLEED. Methods: Forty-eight male Wistar rats (280 ± 20 grams) were divided into two groups: control group (CG), in which the animals do not receive any type of treatment; and biomaterial group (BG), in which the animals received the HA/PLGA/BLEED scaffold. Critical bone injury was induced in the medial region of the skull calotte with the aid of a trephine drill 8 mm in diameter. The biomaterial was implanted in the form of 1.5-mm thick scaffolds. Serum and calotte were collected at one, three and seven days. Results: Biomaterial had a significant effect on the morphological structure of the bone, accelerating osteoblast activation within three days, without causing exacerbated systemic inflammation. In addition, quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that BG induced upregulation of osteogenic genes such as runt-related transcription factor 2, and stimulated genes of inflammatory pathways such as tumor necrosis factor-α, on the first day without overexpressing genes related to bone matrix degradation, such as tissue inhibitor of metalloproteinases-1 and matrix metalloproteinase-9. Conclusions: The HA/PLGA/BLEED® association can be used as a bone graft to aid bone repair, as it is capable of modulating expression of important genes at this stage of the repair process.

https://doi.org/10.1590/acb383823

14.

Development and validation of a video on first aid for choking incidents in the school environment
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Miranda, Priscila da Silva

; Silva, Liliane Faria da

; Cursino, Emília Gallindo

; Góes, Fernanda Garcia Bezerra

; Pacheco, Sandra Teixeira de Araújo

; Moraes, Juliana Rezende Montenegro Medeiros de

Revista Gaúcha de Enfermagem

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2023, Volume 44 elocation e20220251

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective: To develop and validate an educational video on first aid for choking incidents in children in the school environment. Method: Methodological study, conducted between 2021 and 2022, in six stages: search for themes through interviews with 13 teachers and staff members who work in early childhood education in Niterói; theoretical study; development of the video; validation with 17 expert judges; video adaptation and validation with 17 teachers and staff. The interviews were analyzed using the Iramuteq software and the validation through the concordance index, with a desirable value of 70%. Results: The animated video, lasting 4 minutes and 10 seconds, addressed the identification and management of the child experiencing choking and was validated with a concordance index of 97% among the judges and 96% with target audience. Conclusion: The video is a validated technology that can be used to develop educational practices with professionals in the school environment.

RESUMEN Objetivo: Desarrollar y validar un video educativo sobre primeros auxilios para niños en situaciones de atragantamiento en el ámbito escolar. Método: Estudio metodológico, realizado entre 2021 y 2022, en 6 etapas: búsqueda de temas en entrevistas con 13 profesores y empleados que trabajan en la educación infantil en Niterói; estudio teórico; desarrollo del vídeo; validación con 17 jueces expertos; adaptación y validación del vídeo con 17 profesores y empleados. Las entrevistas se analizaron con ayuda del programa informático Iramuteq y la validación mediante el índice de concordancia, valor deseable 70%. Resultados: El vídeo animado, de 4 minutos y 10 segundos de duración, abordaba la identificación y el manejo del niño en situación de atragantamiento y fue validado con índice de concordancia del 97% entre los jueces y del 96% con público objetivo. Conclusión: El vídeo es una tecnología validada que puede utilizarse para desarrollar prácticas educativas con profesionales en el ámbito escolar.

RESUMO Objetivo: Elaborar e validar um vídeo educativo sobre primeiros socorros à criança em situação de engasgo no ambiente escolar. Método: Estudo metodológico, realizado de 2021 a 2022, em seis etapas: busca de temas por meio de entrevistas com 13 professores e funcionários que atuam na educação infantil, em Niterói; estudo teórico; elaboração do vídeo; validação com 17 juízes especialistas; adequação do vídeo e validação com 17 professores e funcionários. As entrevistas foram analisadas com auxílio do software Iramuteq e a validação por meio do índice de concordância, valor desejável 70%. Resultados: O vídeo animado, com duração de 4 minutos e 10 segundos, abordou a identificação e manejo da criança em situação de engasgo e foi validado com índice de concordância de 97% entre os juízes e 99% com público-alvo. Conclusão: O vídeo elaborado é uma tecnologia validada que pode ser utilizada para desenvolver práticas educativas junto aos profissionais no ambiente escolar.

https://doi.org/10.1590/1983-1447.2023.20220251.en

15.

Simulation-based training in Leprosy: development and validation of a scenario for community health workers
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Souza, Raíssa Silva

; Moreira, Juliana Almeida Menezes

; Dias, Ana Angélica Lima

; Coelho, Angélica da Conceição Oliveira

; Amendoeira, José Joaquim Penedos

; Lanza, Fernanda Moura

Revista Brasileira de Enfermagem

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2023, Volume 76 Suppl. 2 elocation e20230114

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objectives: To build and validate a clinical simulation scenario designed to instruct community health workers (CHWs) in active leprosy case detection. Methods: Methodological study involving the development of a simulated clinical scenario and content validation by experts. The Content Validity Index (CVI) was used to determine the level of agreement among the judging commitee, and a descriptive analysis of their recommendations was performed. Results: A simulated scenario with a simulated participant was developed — a simulation characterized by low complexity, moderate physical/environmental fidelity, moderate to high psychological fidelity, and high conceptual fidelity, lasting 50 minutes and capable of training up to 10 CHWs simultaneously. The scenario was validated by 14 experts, with a CVI exceeding 80% for all components. Conclusions: The validated clinical simulation possesses attributes that make it highly reproducible in various national health contexts, thereby contributing to the global “Towards Zero Leprosy” strategy.

RESUMEN Objetivos: Construir y validar escenario de simulación clínica para enseñar a agentes comunitarios de salud (ACS) acciones de búsqueda activa de la lepra. Métodos: Estudio metodológico de construcción de escenario clínico simulado y de validez de contenido por especialistas. Utilizado el Índice de Validez de Contenido (IVC) para determinar el grado de concordancia entre los jueces y el análisis descriptivo de las recomendaciones. Resultados: Creado una simulación escénica con participante simulado, de baja complejidad, mediana fidelidad física/ambiental, mediana/alta fidelidad psicológica y alta fidelidad conceptual, cuyo tiempo de duración total fue de 50 minutos, capaz de calificar hasta diez ACS simultáneamente. Catorce jueces validaron el escenario, obteniéndose IVC superior a 80% en todos los componentes. Conclusiones: La simulación clínica validada tiene atributos que la transforman altamente replicable en diferentes contextos de salud nacionales, pudiendo, de esa manera, contribuir con la estrategia global “Rumbo al cero lepra”.

RESUMO Objetivos: Construir e validar cenário de simulação clínica para ensinar a agentes comunitários de saúde (ACS) ações de busca ativa da hanseníase. Métodos: Estudo metodológico de construção de cenário clínico simulado e de validação de conteúdo por especialistas. Utilizou-se o Índice de Validade de Conteúdo (IVC) para determinar o grau de concordância entre os juízes e a análise descritiva das recomendações. Resultados: Criou-se uma simulação cênica com participante simulado, de baixa complexidade, média fidelidade física/ambiental, média/alta fidelidade psicológica e alta fidelidade conceitual, cujo tempo de duração total é de 50 minutos, capaz de qualificar até dez ACS simultaneamente. Quatorze juízes validaram o cenário, obtendo-se IVC superior a 80% em todos os componentes. Conclusões: A simulação clínica validada tem atributos que a tornam altamente replicável em diferentes contextos de saúde nacionais, podendo, dessa forma, contribuir com a estratégia global “Rumo à zero hanseníase”.

https://doi.org/10.1590/0034-7167-2023-0114

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