Abstract Purpose: To investigate the therapeutic potential of honey, Nigella sativa (N. sativa) and their combination in rat model of excisional wound healing. Methods: A circular excision wound was established in the back region of 50 Wistar rats. Subsequently, they were divided into 5 groups and daily topical administration of lanolin in the control group, honey in the honey group, cold-pressed N. sativa seed oil in the N. sativa groups, mix of 1:1 ratio of honey and N. sativa seed oil in the mix group, and phenytoin cream in the phenytoin group were used. Then, wound surface areas were evaluated using digital camera immediately after the injury and at post excision days 5, 10, 15 and 20. Results: Significant reduction in wound surface area was observed within and between the groups (P < 0.001). In the post excision days 5, 10, 15 and 20 the wound surface areas in the mix group were significantly lower than the other groups followed by the phenytoin, honey, N. sativa, and control groups. Conclusion: The wound healing may be improved and accelerated by using topical solutions of honey, N. sativa seed oil and especially their mixture.
Abstract Purpose: To evaluate the effectiveness of sodium hyaluronate, sesame oil, honey, and silver nanoparticles in preventing of postoperative surgical adhesion formation. Methods: Forty male Wistar rats were randomly assigned into five groups with eight rats in each group including control, hyaluronate, sesame, honey and silver groups. After two weeks the animals underwent laparotomy and were evaluated by two different blinded surgeons for severity of adhesions based on the two different classification scoring systems including Nair classification and cumulative adhesion scoring scale. Results: The scores of severity of adhesions in the hyaluronate and sesame groups were significantly lower than the control group based on the Nair classification (both P-values = 0.02), however based on the cumulative adhesion scoring scale just the score of severity of adhesions in the hyaluronate group was significantly lower than the control group (P-value = 0.02). In the hyaluronate group the severity of adhesions was decreased by 48% based on the cumulative adhesion scoring scale. Conclusions: Sodium hyaluronate and sesame oil may have a significant effect in preventing postoperative surgical adhesion formation.
Abstract: The objective of this work was to evaluate the effects of the integrated application of nitrogen fertilizer and biofertilizers on the yield, grain filling period, and composition of fatty acids of safflower (Carthamus tinctorius). Split-plot experiments were carried out during the 2011 and 2012 crop seasons. The treatments consisted of seed inoculation with plant growth-promoting rhizobacteria (Azotobacter chroococcum strain 5, Azospirillum lipoferum strain F, and Pseudomonas putida strain 186) in the subplots, including a control without seed inoculation; and of the application of N fertilizer at different rates (60, 120, and 180 kg ha-1 urea) in the main plots, including a control without N. The highest grain yield, grain filling period, and effective grain filling period were obtained by the application of 180 kg ha-1 urea and by seed inoculation with P. putida. The application of high N rates and P. putida inoculation resulted in 25.66% increase of the potential rate of grain filling. Biofertilizer inoculation in seed reduced the contents of saturated fatty acids (palmetic and stearic acids) and increased the contents of unsaturated fatty acids (linoleic, linolenic, and oleic acids). The suitable amount of N fertilizer (between 120 and 180 kg ha-1 urea) can improve plant growth, and the quantity and quality of oil in seeds treated with P. putida in safflower plants.
Resumo: O objetivo deste trabalho foi avaliar os efeitos da aplicação integrada de fertilizante nitrogenado e biofertilizantes sobre o rendimento, o período de enchimento de grãos e a composição de ácidos graxos de cártamo (Carthamus tinctorius). Os experimentos foram conduzidos em parcelas subdividas, durante as safras 2011 e 2012. Os tratamentos consistiram da inoculação de rizobactérias promotoras do crescimento de plantas (Azotobacter chroococcum estirpe 5, Azospirillum lipoferum estirpe F e Pseudomonas putida estirpe 186) nas sementes, nas subparcelas, com um controle sem inoculação; e da aplicação de diferentes níveis de adubação nitrogenada (60, 120 e 180 kg ha-1 de ureia) nas parcelas principais, com um controle sem aplicação de N. O maior rendimento de grãos, o maior período de enchimento de grãos e o período efetivo de enchimento de grãos foram obtidos com a aplicação de 180 kg ha-1 de ureia e com a inoculação de P. putida nas sementes. A aplicação de altas doses de N e a inoculação com P. putida resultaram no aumento de 25,66% da taxa potencial de enchimento de grãos. A inoculação de biofertilizantes nas sementes reduziu o teor de ácidos graxos saturados (ácidos palmítico e esteárico) e incrementou os ácidos graxos insaturados (ácidos linoleico, linolênico e oleico). A aplicação da quantidade adequada de adubação nitrogenada (120 a 180 kg ha-1 de ureia) pode melhorar o crescimento de plantas e a quantidade e a qualidade do óleo das sementes tratadas com P. putida, em plantas de cártamo.