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ABSTRACT: Aujeszky’s Disease (AD) is a highly infectious swine disease caused by Suid herpesvirus 1 (SuHV 1). The present study evaluated the biosafety of commercial swine farms and carried out the first serological survey for Aujeszky’s Disease in Espírito Santo State, Brazil. A total of 55 farms in 27 municipalities of the state were selected, where 416 swine serum samples were collected to be submitted to serological diagnosis for AD. Finally, a survey was carried out using a questionnaire to obtain information on the biosecurity of the farms. The results by the ELISA technique indicated the absence of antibodies for AD. Regarding the biosecurity of the farms, 56.4% of the farms did not meet the requirement of internal isolation, 67.3% did not have changing rooms, 72.7% did not provide specific clothing for employees and visitors, 85.5% did not require visitors bathe to access the sheds and only 10.9% of the farms controlled the flow of visitors and vehicles accessing the property. Another noteworthy factor is that only 25.5% of the farms had an area to quarantine replacement animals and 69.1% had an access ramp located close to the animal pens. With the results, it was concluded that there is a need to improve biosecurity measures on commercial farms in the state of Espírito Santo and that there was no presence of Aujeszky’s Disease in the evaluated animals.

RESUMO: A Doença de Aujeszky (DA) é uma enfermidade de suínos altamente infecciosa, causada pelo herpesvírus suíno 1 (SuHV 1). O presente estudo objetivou avaliar a biosseguridade das granjas comerciais capixabas de suínos e realizar o primeiro inquérito sorológico para Doença de Aujeszky no Estado do Espírito Santo, Brasil. Foram selecionadas 55 granjas em 27 municípios do Estado, onde se coletou 416 amostras de soro de suínos para serem submetidos ao diagnóstico sorológico para DA. Por fim, foi realizado inquérito, mediante questionário para obter informações sobre a biosseguridade das granjas. Os resultados pela técnica de ELISA indicaram ausência de anticorpos para DA. Em relação à biosseguridade das granjas, 56,4% das granjas não atenderam o requisito de cerca de isolamento interno, 67,3% não possuíam vestiários, 72,7% não disponibilizavam roupas específicas para funcionários e visitantes, 85,5% não exigiam a prática de banho dos visitantes para acesso aos galpões e somente 10,9% das granjas faziam o controle do fluxo de visitantes e veículos que acessam a propriedade. Outro fator destacado é que apenas 25,5% das granjas apresentavam área para realizar quarentena dos animais de reposição e 69,1% possuíam o embarcador localizado próximo às baias dos animais. Com os resultados, concluiu-se que há necessidade de melhoria das medidas de biosseguridade nas granjas comerciais no Estado do Espírito Santo e que não houve presença da Doença de Aujeszky nos animais avaliados.

https://doi.org/10.1590/0103-8478cr20230037

Therapeutic Drug Monitoring in Inflammatory Bowel Disease
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Lins Neto, Manoel Alvaro de Freitas

; Rolim, João Otávio Moraes

; Jatobá, Diogo César Maurício de Oliveira

; Meira, Júnia Elisa Carvalho de

; Salvador Filho, Luís Henrique

; Lins, Lucas Correia

; Peçanha, Jorge Artur Coelho

Journal of Coloproctology (Rio de Janeiro)

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dec 2023, Volume 43 N. 4 Pages 276 - 279

Abstract: En | Text: En | PDF: En

Abstract Inflammatory bowel disease (IBD) is a problem that directly affects the quality of life of patients suffering from this condition. Monitoring the serum level of infliximab (IFX) (TDM) is an important tool for guiding therapeutic decisions in IBD patients. The purpose of this study was to determine the significance of quantitatively measuring the serum level of IFX (TDM) and antibody to IFX (ATI). Methods and materials: Prospective observational study involving 40 IBD patients on IFX therapy, including 14 Proactive (week 06 of the induction phase) and 26 Reactive (maintenance phase). Immediately prior to the infusion, blood samples were drawn and measured using a Bulhlmann rapid test instrument. Serum concentrations of IFX were categorized as supratherapeutic (>7.0 micrograms/ml), therapeutic (between 3.0 and 7.0 micrograms/ml), and subtherapeutic (3.0 micrograms/ml). When the serum concentration of IFX was 3 mcg/ml (subtherapeutic), the ATI was measured. 25 patients with CD and 15 patients with UC were evaluated. Only three of the twenty patients with subtherapeutic serum levels had a positive ATI, and both were reactive; two had CD and one had UC. There was a statistically significant difference between reactive and proactive patients with respect to levels of CRP (p = 0.042), with proactive DNS patients suffering greater alterations in CRP and albumin.

https://doi.org/10.1055/s-0043-1776892

Diferencias en la gravedad del choque hemorrágico en mujeres con placenta previa y acretismo placentario antes y durante la pandemia de COVID-19
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Nares Torices, Miguel Ángel

; Hernández Pacheco, José Antonio

; Ramírez Calvo, José Antonio

; Flores Cortés, Mildred

; Becerra Navarro, Norma Patricia

; Martínez Hueyopa, Heidi Guadalupe

; Balladares Nava, Elisa Madai

; Serna-Castellanos, Karla Estela

; Brito Brito, Benjamín

; Vila Herrera, Salvador

; Rangel Vargas, Uriel Emmanuel

; Auqui Valarezo, Sandra Verónica

; Mota Calzada, Javier

Medicina crítica (Colegio Mexicano de Medicina Crítica)

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2023, Volume 37 N. 1 Pages 31 - 34

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen: Introducción: estudios previos han mostrado una mayor incidencia de todas las causas de hemorragia postparto durante la pandemia de COVID-19. El acretismo placentario es una causa frecuente de hemorragia postparto. Objetivo: determinar las diferencias en la severidad del choque hemorrágico y la cantidad de sangrado en mujeres con diagnóstico del espectro placenta previa-acretismo, antes de la pandemia y durante la pandemia por COVID-19. Material y métodos: en un estudio con cohortes comparativas de mujeres con placenta previa-acretismo, atendidas en el Instituto Nacional de Perinatología, durante el periodo de enero de 2017 a diciembre de 2019, grupo I (prepandemia), y de enero de 2020 a marzo de 2022, grupo II (pandemia), se comparó entre los grupos la cantidad de hemorragia y la clase del choque hemorrágico de acuerdo a ATLS. Resultados: se estudió a 277 mujeres, 211 fueron del grupo I (pre-COVID-19) y 66 del grupo II (COVID-19), se observó una mayor cantidad de sangrado durante la pandemia de COVID versus la fase pre-COVID (2,150.56 ± 1,910.08 mL versus 1,246.34 ± 1,494.1 mL) p = 0.001, existieron diferencias en las proporciones de la gravedad del choque hemorrágico en la fase de pandemia para las clases III y IV. Conclusión: se encontró un incremento en la cantidad de sangrado y gravedad del choque durante la pandemia de COVID-19.

Abstract: Introduction: previous studies have shown an increased incidence of all-cause postpartum hemorrhage during the COVID-19 pandemic. Placental accreta is a frequent cause of hemorrhagic shock. Objective: determine the severity of shock and the amount of bleeding during the COVID-19 pandemic in women diagnosed with placenta previa and placental accreta. Material and methods: in a comparative cohort study of women with placenta previa and accreta treated at the National Institute of Perinatology during the period from January 2017 to December 2019 group I (pre-pandemic) and from January 2020 to March 2022 group II (pandemic) the amount of hemorrhage and the class of hemorrhagic shock according to ATLS were compared between the groups. Results: 277 women were studied, 211 were from group I (pre COVID-19) and 66 from group II (COVID-19). A greater amount of bleeding was observed during the COVID pandemic versus the pre COVID phase (2,150.56 ± 1,910.08 mL vs 1,246.34 ± 1,494.1 mL) p = 0.001, there were differences in the proportions of severity of hemorrhagic shock in the pandemic phase for classes III and IV. Conclusion: an increase in the amount of bleeding and severity of shock was found during the COVID-19 pandemic.

Resumo: Introdução: estudos anteriores mostraram uma maior incidência de hemorragia pós-parto de todas as causas durante a pandemia de COVID-19. O acretismo placentário é uma causa frequente de hemorragia pós-parto. Objetivo: determinar as diferenças na gravidade do choque hemorrágico e na quantidade de sangramento em mulheres diagnosticadas com o espectro de placenta prévia-acretismo antes da pandemia e durante a pandemia de COVID-19. Material e métodos: em um estudo de coorte comparativo de mulheres com placenta prévia e acreta tratadas no Instituto Nacional de Perinatologia durante o período de janeiro de 2017 a dezembro de 2019, grupo I (pré-pandemia) e de janeiro de 2020 a março de 2022, grupo I II (pandemia) a quantidade de sangramento e a classe de choque hemorrágico de acordo com o ATLS foram comparadas entre os grupos. Resultados: foram estudadas 277 mulheres, 211 eram do grupo I (pré-COVID-19) e 66 do grupo II (COVID-19). Observou-se uma maior quantidade de sangramento durante a pandemia de COVID versus a fase pré-COVID (2,150.56 ± 1,910.08 mL vs 1,246.34 ± 1,494.1 ml) p = 0.001, houve diferenças nas proporções da gravidade do choque hemorrágico na fase pandêmica para as classes III e IV. Conclusão: um aumento na quantidade de sangramento e gravidade do choque foi encontrado durante a pandemia de COVID-19.

https://doi.org/10.35366/109960

Quality of automatic geocoding tools: a study using addresses from hospital record files in Temuco, Chile
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Quinteros, Maria Elisa

; Blazquez, Carola

; Rosas, Felipe

; Ayala, Salvador

; García, Ximena Marcela Ossa

; Delgado-Saborit, Juana Maria

; Harrison, Roy M.

; Ruiz-Rudolph, Pablo

; Yohannessen, Karla

Cadernos de Saúde Pública

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2022, Volume 38 N. 1 elocation e00288920

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo: Os métodos de geocodificação automática se tornaram populares nos últimos anos para facilitar o estudo da associação entre desfechos de saúde e lugar de residência. Entretanto, poucos estudos avaliaram a qualidade da geocodificação, e a maioria dos estudos existentes foi realizada nos Estados Unidos e Europa. O estudo teve como objetivo comparar a qualidade de três ferramentas de geocodificação eletrônica automática em relação a um método de referência. Foi geocodificada uma subamostra de 300 endereços anotados à mão em prontuários hospitalares, usando Bing, Google Earth e Google Maps. As taxas de correspondência dos registros foram mais altas (> 80%) com Google Maps e Google Earth, comparado com Bing. Entretanto, a acurácia dos endereços foi melhor com Bing, com uma proporção maior (> 70%) de endereços com erros de localização menores que 20 metros. Em geral, o desempeno não variou para cada método de acordo com condição socioeconômica. Os métodos apresentaram desempenho geral aceitável, porém heterogêneo. Os resultados servem de alerta contra o uso de métodos automáticos sem avaliar a qualidade em outras cidades, particularmente no Chile e no resto da América Latina.

Abstract: Automatic geocoding methods have become popular in recent years, facilitating the study of the association between health outcomes and the place of living. However, rather few studies have evaluated geocoding quality, with most of them being performed in the US and Europe. This article aims to compare the quality of three automatic online geocoding tools against a reference method. A subsample of 300 handwritten addresses from hospital records was geocoded using Bing, Google Earth, and Google Maps. Match rates were higher (> 80%) for Google Maps and Google Earth compared with Bing. However, the accuracy of the addresses was better for Bing with a larger proportion (> 70%) of addresses with positional errors below 20m. Generally, performance did not vary for each method for different socioeconomic status. Overall, the methods showed an acceptable, but heterogeneous performance, which may be a warning against the use of automatic methods without assessing quality in other municipalities, particularly in Chile and Latin America.

Resumen: Los métodos automáticos de geocodificación se han convertido en algo popular durante los últimos años para facilitar el estudio de la asociación entre resultados de salud y lugar para vivir. No obstante, más bien pocos estudios han evaluado la calidad de la geocodificación, siendo realizados la mayoría de ellos en EE.UU. y Europa. El objetivo de este artículo es comparar la calidad de tres herramientas automáticas de geocodificación en línea frente a un método de referencia. La submuestra de 300 direcciones escritas a mano, procedentes del registro hospitalario, se geocodificaron usando Bing, Google Earth y Google Maps. Los porcentajes de coincidencia fueron mayores (> 80%) en el caso de Google Maps y Google Earth comparados con Bing. Sin embargo, la precisión de las direcciones fue mejor con Bing, en una proporción más grande (> 70%) de direcciones que tenían errores de posición por debajo de 20m. En general, el rendimiento no varió en cada método para diferentes niveles estatus socioeconómico. En general, los métodos mostraron un rendimiento aceptable, pero heterogéneo. Esto previene contra el uso de métodos automáticos sin evaluar la calidad en otras ciudades, particularmente en Chile y Latinoamérica.

https://doi.org/10.1590/0102-311x00288920

Quality of automatic geocoding tools: a study using addresses from hospital record files in Temuco, Chile
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Quinteros, Maria Elisa

; Blazquez, Carola

; Rosas, Felipe

; Ayala, Salvador

; García, Ximena Marcela Ossa

; Delgado-Saborit, Juana Maria

; Harrison, Roy M.

; Ruiz-Rudolph, Pablo

; Yohannessen, Karla

Cadernos de Saúde Pública

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2022, Volume 38 N. 1 elocation e00288920

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.1590/0102-311x00288920

Desigualdades sociales en el consumo de verduras y frutas según características de los hogares argentinos
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Ballesteros, Matías Salvador

; Zapata, María Elisa

; Freidin, Betina

; Tamburini, Camila

; Rovirosa, Alicia

Salud Colectiva

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2022, Volume 18 elocation e3835

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Low consumption of fruits and vegetables contributes to an increased burden of non-communicable diseases. In order to analyze the impact of different factors on fresh fruit and vegetable consumption, an observational, correlational, and cross-sectional study was conducted using data collected in the 2017-2018 National Household Expenditure Survey. We calculated apparent intake of fresh fruits and vegetables in net grams. Descriptive bivariate and multivariate analyses were conducted using multiple linear regression. Apparent intake of fresh fruits and vegetables was well below the recommended level; the lowest consumption was observed in households with lower incomes, with lower educational levels, without elderly members, with children under age 14, with a male head of household, and those residing in the Argentine Northeast region. Identifying social inequalities in food consumption contributes to the development of policies aimed at promoting food and nutrition security.

RESUMEN El bajo consumo de verduras y frutas contribuye al aumento de la carga de enfermedades no transmisibles. Con el objetivo de analizar cómo inciden distintos factores en el consumo de verduras y frutas frescas se realizó un estudio observacional, correlacional y transversal con datos obtenidos de la Encuesta Nacional de Gastos de los Hogares 2017-2018. Se estimó el consumo aparente de verduras y frutas frescas, en gramos de peso neto. Se realizó un análisis descriptivo bivariado y uno multivariado a partir de regresiones lineales múltiples. El consumo aparente de verduras y frutas frescas está muy por debajo de lo recomendado, los menores consumos se observaron en hogares de menores ingresos, con menor clima educativo, sin adultos mayores, con menores de 14 años, con jefatura masculina y que residen en la región del nordeste argentino. Conocer las desigualdades sociales en el consumo contribuye a la planificación de políticas que garanticen la seguridad alimentaria y nutricional.

https://doi.org/10.18294/sc.2022.3835

Desigualdades sociales en el consumo de verduras y frutas según características de los hogares argentinos
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Ballesteros, Matías Salvador

; Zapata, María Elisa

; Freidin, Betina

; Tamburini, Camila

; Rovirosa, Alicia

Salud colectiva

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2022, Volume 18 elocation e3835

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

https://doi.org/10.18294/sc.2022.3835 55 downloads

Determinación de aflatoxinas en especias, ingredientes y mezclas de especias usados en la formulación de productos cárnicos comercializados en la Ciudad de México
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Ríos Barragán, Montserrat Lizeth

; González Sánchez, José Fernando

; Gutiérrez Tolentino, Rey

; Escobar Medina, Arturo Camilo

; Pérez González, José Jesús

; Vega y León, Salvador

Revista mexicana de ciencias pecuarias

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sep 2021, Volume 12 N. 3 Pages 944 - 957

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Aflatoxins are toxic substances produced by some species of fungi that pose a serious danger to human health, especially aflatoxin B1, which is one of the main analytes found in foods and is classified as carcinogenic. The objective of this study was to provide information on the presence of total aflatoxins in spices, ingredients and spice mixtures used for the formulation of meat products and meat products marketed in Mexico City, using an enzyme-linked immunosorbent assay (ELISA) method. Fifty samples were analyzed for total aflatoxins. Sixty-one percent of spices and ingredients were positive for total aflatoxins in concentrations of 0.07 to 4.24 μg/kg; 75 % of spice mixtures were positive, in quantities of 0.6 to 1.9 μg/kg and only 3.5 % of meat products were positive for total aflatoxins. The samples with the highest prevalence of total aflatoxins were chili and paprika. All results showed concentrations below the maximum limit set by the European Union of 10 to 20 μg/kg for total aflatoxins, which does not constitute a current public health problem under the conditions analyzed. It is recommended to use the ELISA system as a screening method and subsequent confirmation by liquid chromatography with fluorescence detection, as well as to have a monitoring program to evaluate the presence of aflatoxins and other mycotoxins; there is also a need for an official Mexican regulation for mycotoxins in spices considering the high consumption of chili in the Mexican population.

Resumen Las aflatoxinas son sustancias tóxicas producidas por algunas especies de hongos que suponen un grave peligro para la salud humana, en especial la aflatoxina B1 que es uno de los principales analitos encontrados en alimentos y está catalogado como cancerígeno. El objetivo de este estudio fue proporcionar información sobre la presencia de aflatoxinas totales en especias, ingredientes y mezclas de especias usados para la formulación de productos cárnicos y productos cárnicos comercializados en la Ciudad de México, empleando un método de ensayo por inmunoabsorción ligado a enzimas (ELISA). Se analizaron 50 muestras contra aflatoxinas totales. El 61 % de especias e ingredientes fueron positivas a aflatoxinas totales en concentraciones de 0.07 a 4.24 µg/kg; las mezclas de especias fueron positivas el 75 %, en cantidades de 0.6 a 1.9 µg/kg y los productos cárnicos sólo el 3.5 % fueron positivos a aflatoxinas totales. Las muestras con mayor prevalencia de aflatoxinas totales fueron chile y pimentón. Todos los resultados presentaron concentraciones inferiores al límite máximo establecido por la Unión Europea de 10 a 20 µg/kg para aflatoxinas totales; lo que no constituye un problema de salud pública actual en las condiciones analizadas. Se recomienda el uso del sistema ELISA como método de pesquisaje y posterior confirmación por cromatografía liquida con detección fluorescente, así como contar con un programa de monitoreo para evaluar la presencia de aflatoxinas y otras micotoxinas; además existe la necesidad de una regulación oficial mexicana para micotoxinas en especias considerando el alto consumo de chile en la población mexicana.

https://doi.org/10.22319/rmcp.v12i3.5530 91 downloads

Evitemos la rutina: situs inversus
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Blanco González, Javier

; Navarro Salvador, Lucía

; Castaño Andreu, Elisa

; Checa Díaz, Patricia

; Gilarte Herrera, Cristina E

; Adrados Razola, Ignacio

Pediatría Atención Primaria

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2020, Volume 22 Suppl. 28 Pages 152 - 153

Abstract: Es | Text: Es | PDF: Es

53 downloads

10.

Brazilian Nutritional Consensus in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease
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Pereira, Andréa Z

; Vigorito, Afonso Celso

; Almeida, Alessandro de Moura

; Candolo, Alexandre de Almeida

; Silva, Ana Carolina Leão

; Brandão-Anjos, Ana Elisa de Paula

; Sá, Bianca Laselva de

; Souza, Catarina Lôbo Santos de

; Castro Junior, Cláudio Galvão de

; Oliveira, José Salvador Rodrigues de

; Barban, Juliana Bernardo

; Mancilha, Elaine Maria Borges

; Todaro, Juliana

; Lopes, Lilian Pinheiro

; Macedo, Maria Cristina Martins de Almeida

; Rodrigues, Morgani

; Ribeiro, Paulo Cesar

; Silva, Roberto Luiz da

; Roberto, Telma Sigolo

; Rodrigues, Thays de Cássia Ruiz

; Colturato, Vergilio Antonio Rensi

; Paton, Eduardo José de Alencar

; Barros, George Maurício Navarro

; Almeida, Rosana Ducatti Souza

; Moreira, Maria Claudia Rodrigues

; Flowers, Mary Evelyn

einstein (São Paulo)

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2020, Volume 18 elocation eAE4799

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O Consenso Brasileiro de Nutrição no Transplante de Células Tronco Hematopoiéticas: doença do enxerto contra o hospedeiro foi aprovado pela Sociedade Brasileira de Transplante de Medula Óssea, com a participação de 26 centros brasileiros de transplante de células-tronco hematopoiéticas. O Consenso descreve as principais condutas nutricionais em casos de doença do enxerto contra o hospedeiro, a principal complicação do transplante de células-tronco hematopoiéticas.

ABSTRACT The Brazilian Consensus on Nutrition in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease was approved by Sociedade Brasileira de Transplante de Medula Óssea , with the participation of 26 Brazilian hematopoietic stem cell transplantation centers. It describes the main nutritional protocols in cases of Graft- versus -host disease, the main complication of hematopoietic stem cell transplantation.

https://doi.org/10.31744/einstein_journal/2020ae4799 1552 downloads

11.

Neonatal Screening for Congenital Hypothyroidism in Nicaragua: Audit of a Cord-blood Thyrotropin-based Program (2005-2015)
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Fúnez, Aura

; Lara, María Eugenia

; Chévez, Ana Cecilia

; Castellón, Efrén Alí

; Perán, Salvador

; Toro, María Josefa

; Montoya, Eladio

; varro, José Carlos Moreno

Journal of Inborn Errors of Metabolism and Screening

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2019, Volume 7 elocation e2019003

Abstract: En | Text: En | PDF: En

Abstract The aim of this study is to evaluate the Nicaraguan screening program for congenital hypothyroidism in terms of coverage and effectiveness of detection and confirmation of cases with the condition throughout a decade. Thyrotropin was quantified in cord-blood samples by a validated ELISA and a cut-off of 20 mU/l was applied. Coverage, positive predictive value, recall rate and prevalence were retrospectively analysed. Babies with positive screening results were contacted for confirmation by means of determination of thyrotropin and thyroid profile in serum samples. 272,338 babies were screened during the period 2005-2015. The mean coverage reached by the program in the participating departments was 71%, with a positive predictive value of 83% and a recall rate of 0.055%. Eighty cases of congenital hypothyroidism were identified, representing an incidence of 1 in 3229 live births, most of them (81%) being severe. The performance of the Nicaraguan screening program is comparable to those in Latin America also using cord-blood samples. The incidence of congenital hypothyroidism is within the low range of other countries worldwide. Strategies are needed to expand the program to the whole country, improve recall rates and achieve earlier treatment of babies, with the condition.

https://doi.org/10.1590/2326-4594-jiems-2019-0003 1929 downloads

12.

Vitamin D status by sociodemographic factors and body mass index in Mexican women at reproductive age
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Contreras-Manzano, Alejandra

; Villalpando, Salvador

; Robledo-Pérez, Ricardo

Salud Pública de México

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oct 2017, Volume 59 N. 5 Pages 518 - 525

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: Objective: To describe the prevalence of Vitamin D deficiency (VDD) and insufficiency (VDI), and the main dietary sources of vitamin D (VD) in a probabilistic sample of Mexican women at reproductive age participating in Ensanut 2012, stratified by sociodemographic factors and body mass index (BMI) categories. Materials and methods: Serum concentrations of 25-hydroxyvitamin-D(25-OH-D) were determined using an ELISA technique in 4162 women participants of Ensanut 2012 and classified as VDD, VDI or optimal VD status. Sociodemographic, anthropometric and dietary data were also collected. The association between VDD/VDI and sociodemographic and anthropometry factors was assessed adjusting for potential confounders through an estimation of a multinomial logistic regression model. Results: The prevalence of VDD was 36.8%, and that of VDI was 49.8%. The mean dietary intake of VD was 2.56 μg/d. The relative risk ratio (RRR) of VDD or VDI was calculated by a multinomial logistic regression model in 4162 women. The RRR of VDD or VDI were significantly higher in women with overweight (RRR: 1.85 and 1.44, p<0.05), obesity (RRR: 2.94 and 1.93, p<0.001), urban dwelling (RRR:1.68 and 1.31, p<0.06), belonging to the 3rd tertile of income (RRR: 5.32 and 2.22, p<0.001), or of indigenous ethnicity (RRR: 2.86 and 1.70, p<0.05), respectively. Conclusion: The high prevalence of VDD/VDI in Mexican women calls for stronger actions from the health authorities, strengthtening the actual policy of food supplementation and recommending a reasonable amount of sun exposure.

Resumen: Objetivo: Describir la prevalencia de deficiencia (DVD) e insuficiencia (IVD) de vitamina D (VD), y las principales fuentes dietéticas de VD en una muestra probabilística de mujeres mexicanas en edad reproductiva participantes de la Ensanut 2012, estratificando por factores sociodemográficos y categorías de IMC. Material y métodos: Las concentraciones séricas de 25-hidroxivitamina-D (25-OH-D) se midieron utilizando la técnica ELISA en 4162 mujeres participantes de la Ensanut 2012, que fueron clasificadas con DVD, IVD u óptimos niveles de VD. Se recolectaron datos sociodemográficos, antropometría y dieta, y se evaluó su asociación con DVD/IVD por medio de un modelo de regresión logística multinomial. Resultados: 36.8% de las mujeres presentaron DVD y 49.8% IVD. La media de ingesta de VD fue 2.56 μg/d. La probabilidad de presentar DVD o IVD fue mayor en las mujeres con sobrepeso (RRR: 1.85 y 1.44, p<0.05), obesidad (RRR: 2.94 y 1.93, p<0.001), residentes del área urbana (RRR: 1.68 y 1.31, p<0.06), del tercil 3 de nivel socioeconómico (RRR: 5.32 y 2.22, p<0.001) o indígenas (RRR: 2.86 y 1.70, p<0.05) respectivamente. Conclusiones: La alta prevalencia de DVD/IVD en mujeres mexicanas es un llamado a las autoridades de salud para implementar una política de suplementación de alimentos más fuerte y hacer recomendaciones para una razonable exposición al sol.

https://doi.org/10.21149/8080 178 downloads

13.

Vitamin D status by sociodemographic factors and body mass index in Mexican women at reproductive age
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Contreras-Manzano, Alejandra

; Villalpando, Salvador

; Robledo-Pérez, Ricardo

Salud Pública de México

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oct 2017, Volume 59 N. 5 Pages 518 - 525

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

https://doi.org/10.21149/8080 958 downloads

14.

Mitochondrial proteomic profile of complex IV deficiency fibroblasts: rearrangement of oxidative phosphorylation complex/supercomplex and other metabolic pathways
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Salvador-Severo, Karina

; Gómez-Caudillo, Leopoldo

; Quezada, Héctor

; García-Trejo, José de Jesús

; Cárdenas-Conejo, Alan

; Vázquez-Memije, Martha Elisa

; Minauro-Sanmiguel, Fernando

Boletín médico del Hospital Infantil de México

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jun 2017, Volume 74 N. 3 Pages 175 - 180

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: Background: Mitochondriopathies are multisystem diseases affecting the oxidative phosphorylation (OXPHOS) system. Skin fibroblasts are a good model for the study of these diseases. Fibroblasts with a complex IV mitochondriopathy were used to determine the molecular mechanism and the main affected functions in this disease. Methods: Skin fibroblast were grown to assure disease phenotype. Mitochondria were isolated from these cells and their proteome extracted for protein identification. Identified proteins were validated with the MitoMiner database. Results: Disease phenotype was corroborated on skin fibroblasts, which presented a complex IV defect. The mitochondrial proteome of these cells showed that the most affected proteins belonged to the OXPHOS system, mainly to the complexes that form supercomplexes or respirosomes (I, III, IV, and V). Defects in complex IV seemed to be due to assembly issues, which might prevent supercomplexes formation and efficient substrate channeling. It was also found that this mitochondriopathy affects other processes that are related to DNA genetic information flow (replication, transcription, and translation) as well as beta oxidation and tricarboxylic acid cycle. Conclusions: These data, as a whole, could be used for the better stratification of these diseases, as well as to optimize management and treatment options.

Resumen: Introducción: Las mitocondriopatías son enfermedades multisistémicas que afectan el funcionamiento de la fosforilación oxidativa (OXPHOS). Un buen modelo de estudio para estas enfermedades es el cultivo primario de fibroblastos. En este trabajo se utilizaron fibroblastos con mitocondriopatía del complejo IV para determinar cuáles son las principales funciones afectadas en esta enfermedad. Métodos: Se realizaron cultivos primarios de fibroblastos para corroborar el fenotipo de la enfermedad. Las mitocondrias se aislaron de estas células y se extrajo su proteoma para su identificación. Las proteínas identificadas se validaron con la base de datos de MitoMiner. Resultados: Los fibroblastos conservaron el fenotipo de la enfermedad que incluye un defecto del complejo IV. El proteoma mitocondrial de estas células mostró que las proteínas más afectadas pertenecen al sistema de OXPHOS, principalmente los complejos que forman supercomplejos o respirosomas (I, III, IV y V). El defecto en el complejo IV al parecer se debió a problemas de ensamblaje que pueden evitar la formación de los supercomplejos y la eficiente canalización de sustratos. También se observó que esta mitocondriopatía afecta otros procesos relacionados con el flujo de información genética del DNA (replicación, transcripción y traducción), así como con la beta oxidación y el ciclo de los ácidos tricarboxílicos (TCA). Conclusiones: En conjunto, estos datos podrían utilizarse para una mejor clasificación de estas enfermedades, así como para la optimización de las opciones de manejo y tratamiento.

https://doi.org/10.1016/j.bmhimx.2017.05.001 1069 downloads

15.

Use of fish-farming wastewater in lettuce cultivation
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Guimarães, Isaías P.

; Oliveira, Francisco de A. de

; Torres, Salvador B.

; Pereira, Francisco E. C. B.

; França, Francisco D. de

; Oliveira, Mychelle K. T. de

Revista Brasileira de Engenharia Agrícola e Ambiental

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aug 2016, Volume 20 N. 8 Pages 728 - 733

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A alface se destaca como a principal hortaliça folhosa consumida no Brasil apresentando divergência para adaptabilidade a diferentes condições ambientais, entre as quais a salinidade. O trabalho teve, como objetivo, avaliar as respostas de cultivares de alface ao uso de água salina residuária da atividade da piscicultura. O experimento foi desenvolvido sob delineamento inteiramente casualizado, com quatro repetições, em esquema fatorial 7 x 4 sendo sete cultivares (Regiane, Vera, Isabela, Elisa, Amélia, Lavínia e Angelina) e quatro níveis de salinidade (1,2; 2,2; 3,2 e 4,2 dS m-1). As características avaliadas foram: número de folhas, área foliar, diâmetro da coroa, massa fresca e seca da parte aérea. Verificou-se que a água residuária da piscicultura diluída pode ser utilizada na irrigação da alface cultivada em vasos. As cultivares Regiane e Elisa apresentaram maior emissão de folhas e área foliar e as cultivares Amélia e Angelina foram as mais produtivas em termos de biomassa. As cultivares Vera, Isabela, Amélia e Angelina se mostraram mais tolerantes à salinidade da solução de fertirrigação.

ABSTRACT Lettuce stands out as the main leafy vegetable consumed in Brazil, with divergence regarding adaptability to different environmental conditions, which include salinity. The objective of the work was to evaluate the response of lettuce cultivars to the use of saline wastewater from fish farming. The experiment was conducted in a completely randomized design with four replicates, in a factorial 7 x 4, corresponding to seven cultivars (Regiane, Vera, Isabela, Elisa, Amelia, Lavinia and Angelina) and four salt concentrations (1.2, 2.2, 3.2 and 4.2 dS m-1). The evaluated characteristics were: number of leaves, leaf area, crown diameter and shoot fresh and dry matter. Diluted wastewater from fish farming can be used in the preparation of solution for fertigation of lettuce. The cultivars Regiane and Elisa had higher production of leaves and leaf area, while Amélia and Angelina were the most productive in terms of biomass. The cultivars Vera, Isabela, Amélia and Angelina were more tolerant to the salinity of the fertigation solution.

https://doi.org/10.1590/1807-1929/agriambi.v20n8p728-733 1610 downloads

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