ABSTRACT Safflower (Carthamus tinctorius L.) is an annual herbaceous plant, cultivated mainly for the seed which is used for edible oil extraction and bird feeding. This study was designed to evaluate the safety of a new pigmented variety of safflower (A82) seeds. The results showed that oral administration of A82 seeds significantly increased the body weight of male rats in a dose-dependent manner (p<0.05). Biochemical tests showed that A82 seeds significantly increased the serum levels of AST (Aspartate aminotransferase) (p<0.05), slightly reduced the serum levels of ALT (Alanine aminotransferase) and significantly reduced ALP (p<0.05) levels in a dose dependent manner. BUN (Blood Urea Nitrogen) and Cr (Creatinine) were not significantly changed in A82 seed treated groups. Also, testosterone levels were not significantly changed by administration of different doses of A82. However, Johnson scoring showed slightly decrease in experimental groups. No organ weight or histological changes were observed in liver, kidney, spleen, heart and brain of A82 seed treated animals. These results indicate that A82 seeds have not any toxic effects in Wistar rats. Future studies are required to clarify the exact mechanism by which A82 seeds alter AST levels and body weight in rat.