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PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri
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Santos, Ana Paula Maia dos

; Santos, Edson Mauro

; Silva de Oliveira, Juliana

; Garcia Leal de Araújo, Gherman

; de Moura Zanine, Anderson

; Araújo Pinho, Ricardo Martins

; Costa do Nascimento, Thiago Vinicius

; Fernandes Perazzo, Alexandre

; Ferreira, Daniele de Jesus

; da Silva Macedo, Alberto Jefferson

; de Sousa Santos, Francisco Naysson

Revista de la Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo

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jun 2023, Volume 55 N. 1 Pages 115 - 125

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Este estudio tuvo como objetivo evaluar el efecto de la inoculación con L. buchneri liofilizado y/o activado sobre las poblaciones de bacterias de ácido láctico en ensilajes de maíz. Los tratamientos experimentales consistieron en ensilaje de maíz sin aditivo y ensilaje con los inoculantes L. buchneri 1 × 10 5 ufc/g, aplicados según las recomendaciones del fabricante (1 g/tonelada de forraje) en las formas de inoculante liofilizado e inoculante preactivado. Se identificaron aislados purificados de ensilajes de maíz con y sin inocu lación, y el 93% de los aislados correspondieron a bacterias de ácido láctico de la especie L. plantarum. Entre los aislados no se detectaron bacterias de la especie L. buchneri. La aplicación de L. buchneri liofilizado o activado en la masa ensilada promueve un aumento considerable de la población de bacterias de ácido lácticas en los ensilajes de maíz durante todo el período de fermentación.

Abstract This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 10 5 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.

Seroprevalence of SARS-CoV-2 antibodies in schoolchildren in the city of São Paulo, 2020
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Kamioka, Gabriela Akemi

; Madalosso, Geraldine

; Albuquerque, José Olimpio Moura de

; Costa, Selma Anequini

; Ferreira, Paula Bisordi

; Sato, Ana Paula Sayuri

; Glasser, Paula Regina

; Pino, Francisco Alberto

; Burihan, Patrícia Carla Piragibe Ramos

; Carvalho, Ana Carolina Aguiar de

; Amorim, Ana Beatriz Pagliaro

; Cavazzana, Cinthya Luzia

; Aires, Caroline Cotrim

; Kataoka, Ana Paula Arruda Geraldes

; Savani, Elisa San Martin Mouriz

; Bessa, Thirsa Alvares Franco

; Aguiar, Breno Souza de

; Failla, Marcelo Antunes

; Santos, Edson Aparecido dos

; Brito, Edjane Maria Torreão

; Santos, Maria Cristina Honório dos

; Caldeira, Luiz Artur Vieira

; Silva, Solange Maria Saboia e

; Zamarco, Luiz Carlos

; Fonseca, Sandra Maria Sabino

; Lima, Marcia Maria de Cerqueira

; Marques, Ivanilda Argenau

; Mauro, Athenê Maria de Marco França

; Masi, Eduardo de

Revista de Saúde Pública

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2023, Volume 57 Suppl. 1 elocation 6s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO Estimar a soroprevalência de anticorpos do vírus SARS-CoV-2 em escolares de quatro a 14 anos de idade residentes no município de São Paulo, segundo variáveis clínicas, demográficas, epidemiológicas e sociais, durante o período de fechamento das escolas como medida de controle da covid-19. MÉTODOS Realizou-se um inquérito sorológico em setembro de 2020 com amostra aleatória estratificada por tipo de rede de ensino (pública municipal, pública estadual e privada). Foi coletada amostra de sangue venoso utilizando-se o teste de imunoensaio de fluxo lateral da fabricante Wondfo para detecção de anticorpos totais contra o vírus SARS-CoV-2. Aplicaram-se questionários semiestruturados para o levantamento de dados clínicos, demográficos, sociais e epidemiológicos. RESULTADOS A soroprevalência de anticorpos do vírus SARS-CoV-2 em escolares foi de 16,6% (IC95% 15,4–17,8). O estudo encontrou soroprevalências mais elevadas na rede pública municipal (18,5%; IC95% 16,6–20,6) e estadual (16,2%; IC95% 14,4–18,2) em relação à rede privada (11,7; IC95% 10,0–13,7) e entre escolares da raça/cor preta e parda (18,4%; IC95% 16,8–20,2) e no estrato social mais vulnerável (18,5%; IC95% 16,9–20,2). A pesquisa identificou menores soroprevalências nos escolares que relataram seguir as medidas recomendadas de proteção contra a covid-19. CONCLUSÃO A soroprevalência de anticorpos contra o vírus SARS-CoV-2 atinge principalmente os escolares socialmente mais vulneráveis. Este estudo pode contribuir para embasar políticas públicas que reforcem a importância da suspensão das aulas presenciais e da necessidade de estratégias de medidas de proteção e acompanhamento do status sorológico daqueles que ainda não foram contemplados no calendário vacinal.

ABSTRACT OBJECTIVE To estimate seroprevalence of SARS-CoV-2 antibodies in schoolchildren aged 4 to 14 years living in the city of São Paulo, according to clinical, demographic, epidemiological, and social variables, during the school closure period as a measure against covid-19 spread. METHODS A serological survey was made in September 2020 with a random sample stratified by school system (municipal public, state public and private) type. A venous blood sample was collected using the Wondfo SARS-CoV-2 Antibody Test (lateral flow method) for detection of total SARS-CoV-2 virus antibodies. Semi-structured questionnaires were applied to collect clinical, demographic, social, and epidemiological data. RESULTS Seroprevalence of SARS-CoV-2 antibodies in schoolchildren was of 16.6% (95%CI 15.4–17.8). The study found higher seroprevalence in the municipal (18.5%; 95%CI 16.6–20.6) and state (16.2%; 95%CI 14.4–18.2) public school systems compared to the private school system (11.7; 95%CI 10.0–13.7), among black and brown students (18.4%; 95%CI 16.8–20.2) and in the most vulnerable social stratum (18.5 %;95%CI 16.9–20.2). Lower seroprevalence was identified in schoolchildren who reported following the recommended protective measures against covid-19. CONCLUSION Seroprevalence of SARS-CoV-2 antibodies is found mainly in the most socially vulnerable schoolchildren. This study can contribute to support public policies that reinforce the importance of suspending face-to-face classes and developing strategies aimed at protective measures and monitoring of the serological status of those who have not yet been included in the vaccination schedule.

https://doi.org/10.11606/s1518-8787.2023057004782

Seroprevalence of SARS-CoV-2 antibodies in schoolchildren in the city of São Paulo, 2020
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Kamioka, Gabriela Akemi

; Madalosso, Geraldine

; Albuquerque, José Olimpio Moura de

; Costa, Selma Anequini

; Ferreira, Paula Bisordi

; Sato, Ana Paula Sayuri

; Glasser, Paula Regina

; Pino, Francisco Alberto

; Burihan, Patrícia Carla Piragibe Ramos

; Carvalho, Ana Carolina Aguiar de

; Amorim, Ana Beatriz Pagliaro

; Cavazzana, Cinthya Luzia

; Aires, Caroline Cotrim

; Kataoka, Ana Paula Arruda Geraldes

; Savani, Elisa San Martin Mouriz

; Bessa, Thirsa Alvares Franco

; Aguiar, Breno Souza de

; Failla, Marcelo Antunes

; Santos, Edson Aparecido dos

; Brito, Edjane Maria Torreão

; Santos, Maria Cristina Honório dos

; Caldeira, Luiz Artur Vieira

; Silva, Solange Maria Saboia e

; Zamarco, Luiz Carlos

; Fonseca, Sandra Maria Sabino

; Lima, Marcia Maria de Cerqueira

; Marques, Ivanilda Argenau

; Mauro, Athenê Maria de Marco França

; Masi, Eduardo de

Revista de Saúde Pública

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2023, Volume 57 elocation 6s

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

https://doi.org/10.11606/s1518-8787.2023057004782

Diversity of rickettsiae in ticks (Acari: Ixodidae) collected from wild vertebrates in part of the Amazon, Cerrado, and Pantanal biomes in Brazil
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Prati, Anny Carolina

; Maia, Maerle Oliveira

; Martins, Thiago Fernandes

; Morgado, Thaís Oliveira

; Corrêa, Sandra Helena Ramiro

; Mendes, Edson Junior Figueiredo

; Ferraz, Rosa Helena dos Santos

; Mudrek, Jessica Rhaiza

; Strüssmann, Christine

; Ramos, Dirceu Guilherme de Souza

; Semedo, Thiago Borges Fernandes

; Minetto, Make Kawatake

; Aguiar, Daniel Moura de

; Pacheco, Richard Campos

; Melo, Andréia Lima Tomé

Revista Brasileira de Parasitologia Veterinária

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2023, Volume 32 N. 4 elocation e008023

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Ticks parasitizing 102 wild animals in the states of Mato Grosso and Goiás, Brazil were collected between 2015 and 2018. A total of 2338 ticks (865 males, 541 females, 823 nymphs, and 109 larvae) belonging to four genera (Amblyomma, Dermacentor, Haemaphysalis, and Rhipicephalus) and at least 21 species were identified. DNA extraction and a molecular survey for rickettsial agents were performed on 650 ticks. The results revealed parasitism by the following species: Rickettsia amblyommatis in Amblyomma cajennense s.s., A. cajennense s.l., Amblyomma coelebs, Amblyomma humerale, Amblyomma longirostre, Amblyomma nodosum, Amblyomma scalpturatum, Amblyomma sculptum, and Amblyomma romitii; Rickettsia parkeri in Amblyomma nodosum, Amblyomma ovale, Amblyomma scalpturatum, and Amblyomma triste; Rickettsia rhipicephali in Haemaphysalis juxtakochi; Rickettsia sp. in A. cajennense s.s., A. nodosum, and A. sculptum, and lastly, ‘Candidatus Rickettsia andeanae’ in Amblyomma parvum and Rhipicephalus microplus. This study expands the body of knowledge about tick parasitism among wild animals, including new data concerning tick-host associations, and provides information about the epidemiology of tick-borne pathogens in the Center-West region of Brazil.

Resumo Foram coletados carrapatos parasitando 102 animais silvestres nos estados de Mato Grosso e Goiás, Brasil, entre 2015 e 2018. Um total de 2338 carrapatos (865 machos, 541 fêmeas, 823 ninfas e 109 larvas) pertencentes a quatro gêneros (Amblyomma, Dermacentor, Haemaphysalis e Rhipicephalus) e pelo menos 21 espécies foram identificadas. A extração de DNA e pesquisa molecular para agentes riquétsiais foram realizadas em 650 carrapatos. Os resultados revelaram parasitismo pelas seguintes espécies: Rickettsia amblyommatis em Amblyomma cajennense s.s., A. cajennense s.l., Amblyomma coelebs, Amblyomma humerale, Amblyomma longirostre, Amblyomma nodosum, Amblyomma scalpturatum, Amblyomma sculptum e Amblyomma romitii; Rickettsia parkeri em Amblyomma nodosum, Amblyomma ovale, Amblyomma scalpturatum e Amblyomma triste; Rickettsia rhipicephali em Haemaphysalis juxtakochi; Rickettsia sp. em A. cajennense s.s., A. nodosum e A. sculptum e, por último, ‘Candidatus Rickettsia andeanae’ em Amblyomma parvum e Rhipicephalus microplus. Este estudo amplia o conhecimento sobre o parasitismo de carrapatos em animais silvestres, incluindo-se novos dados sobre associações carrapato-hospedeiro. Fornece, ainda, informações sobre a epidemiologia de patógenos transmitidos por carrapatos na região Centro-Oeste do Brasil.

https://doi.org/10.1590/s1984-29612023059

Performance of lambs fed total feed silage based on cactus pear
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Silva, Juscelino Kubitschek Bevenuto da

; Araújo, Gherman Garcia Leal de

; Santos, Edson Mauro

; Oliveira, Juliana Silva de

; Campos, Fleming Sena

; Gois, Glayciane Costa

; Silva, Tiago Santos

; Matias, Alex Gomes da Silva

; Ribeiro, Ossival Lolato

; Perazzo, Alexandre Fernandes

; Zanine, Anderson de Moura

Revista mexicana de ciencias pecuarias

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mar 2022, Volume 13 N. 1 Pages 19 - 31

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen: Este estudio tuvo como objetivo evaluar la ingesta, digestibilidad aparente, balance hídrico, balance de nitrógeno y rendimiento productivo en corderos alimentados con ensilaje de nopal asociado con forrajes tropicales. En el experimento se utilizaron 40 corderos machos cruzados intactos, con un peso corporal inicial de 18.85 ± 1.2 kg. El diseño experimental fue completamente al azar, con 5 tratamientos y 8 repeticiones. Los tratamientos consistieron en dietas con diferentes proporciones de pasto buffel y salvado de trigo como fuentes de fibra detergente neutro (FDN), en ensilaje de alimento total (EAT) a base de nopal: EAT1 - 279 g/kg de pasto buffel; EAT2 - 240 g/kg de pasto buffel y 17 g/kg de salvado de trigo; EAT3 - 198 g/kg de pasto buffel y 34 g/kg de salvado de trigo; EAT4 - 108 g/kg de pasto buffel y 74 g/kg de salvado de trigo; EAT5 - 118 g/kg de salvado de trigo. La ingesta más baja de FDN se encontró en corderos que consumieron EAT5 (402 g/día). La digestibilidad aparente de los carbohidratos no fibrosos fue mayor para EAT5, mientras que la digestibilidad aparente de FDN de EAT5 y EAT4 fue mayor que la de EAT1. Las dietas promovieron una ganancia diaria de peso promedio de 180.8 g/día. En condiciones experimentales, el uso de nopal forrajero y concentrado en forma de raciones mixtas totales de ensilaje conduce a una mayor ingesta de proteína cruda, carbohidratos no fibrosos, extracto etéreo y mayor digestibilidad de carbohidratos no fibrosos y fibra detergente neutro, sin embargo, todas las dietas fueron viables en la alimentación de ovinos confinados, proporcionando ganancias de hasta 198 g/d según la formulación de la dieta.

Abstract This study aimed was to evaluate the intake, apparent digestibility, water balance, nitrogen balance, and productive performance in lamb fed cactus pear silage associated with tropical forages. Forty male intact crossbred lambs, with an initial body weight of 18.85 ± 1.2 kg, were used in the experiment. The experimental design was completely randomized, with 5 treatments and 8 replications. The treatments consisted of diets with different proportions of buffelgrass and wheat bran as sources of neutral detergent fiber (NDF), in total feed silage (TFS) based on cactus pear: TFS1 - 279 g/kg of buffelgrass; TFS2 - 240 g/kg of buffelgrass and 17 g/kg of wheat bran; TFS3 - 198 g/kg of buffelgrass and 34 g/kg of wheat bran; TFS4 - 108 g/kg of buffelgrass and 74 g/kg of wheat bran; TFS5 - 118 g/kg of wheat bran. The lowest NDF intake was found in lambs that consumed TFS5 (402 g/d). The non-fibrous carbohydrates apparent digestibility was higher for TFS5, while the NDF apparent digestibility of TFS5 and TFS4 was higher than TFS1. Diets promoted an average daily weight gain of 180.8 g/d. Under experimental conditions, the use of forage cactus pear and concentrate in the form of total mixed rations silage leads to greater intake of crude protein, non-fibrous carbohydrates, ether extract and greater digestibility of non-fibrous carbohydrates and neutral detergent fiber, however, all diets were viable in the feed of confined sheep, providing gains of up to 198 g/d according with the formulation of the diet.

https://doi.org/10.22319/rmcp.v13i1.5849 30 downloads

Prevalence evolution of SARS-CoV-2 infection in the city of São Paulo, 2020–2021
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Albuquerque, José Olimpio Moura de

; Kamioka, Gabriela Akemi

; Madalosso, Geraldine

; Costa, Selma Anequini

; Ferreira, Paula Bisordi

; Pino, Francisco Alberto

; Sato, Ana Paula Sayuri

; Carvalho, Ana Carolina Aguiar de

; Amorim, Ana Beatriz Pagliaro

; Aires, Caroline Cotrim

; Kataoka, Ana Paula Arruda Geraldes

; Savani, Elisa San Martin Mouriz

; Bessa, Thirsa Alvares Franco

; Aguiar, Breno Souza de

; Failla, Marcelo Antunes

; Santos, Edson Aparecido dos

; Brito, Edjane Maria Torreão

; Santos, Maria Cristina Honório dos

; Silva, Solange Maria Saboia e

; Caldeira, Luiz Artur Vieira

; Zamarco, Luiz Carlos

; Fonseca, Sandra Maria Sabino

; Lima, Marcia Maria de Cerqueira

; Marques, Ivanilda Argenau

; Silva, Fabiana Érica Vilanova da

; Glasser, Paula Regina

; Burihan, Patrícia Carla Piragibe Ramos

; Cavazzana, Cinthya Luzia

; Mello, Debora Silva de

; Pellini, Alessandra Cristina Guedes

; Nishio, Fernando Yoshiki

; Kian, Fernanda Miyashiro

; Braga, Elza de Santana

; Bertelli, Nilza Maria Piassi

; Fracini, Wagner

; Gonçalves, Marcelo Dell Áquila

; Zular, Paulete Secco

; Piva, Regiane de Santana

; Masi, Eduardo

Revista de Saúde Pública

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2021, Volume 55 elocation 62

Abstract: En | Text: En | PDF: En

ABSTRACT OBJECTIVE To estimate the evolution of the prevalence of SARS-CoV-2 virus infection among residents aged 18 years or over in the municipality of São Paulo. METHODS This is a population-based household survey conducted every 15 days, between June and September 2020, and January and February 2021. In total, the study comprised 11 phases. The presence of antibodies against SARS-CoV-2 was identified in venous blood using a lateral flow test, Wondfo Biotech. In the last phase, the researchers combined it with an immunoenzymatic test, Euroimmun. The participants also answered a semi-structured questionnaire on sociodemographic and economic factors, and on social distancing measures. Prevalence estimates and the 95% confidence interval were estimated according to regions, Human Development Index, sex, age group, ethnicity, education, income, and variables associated with risk or prevention of infection. To compare the frequencies among the categories of each variable, the chi-square test with Rao-Scott correction was used, considering a significance level of 5%. RESULTS In total, 23,397 individuals were interviewed and had their samples collected. The estimated prevalence of antibodies against SARS-CoV-2 ranged from 9.7% (95%CI: 7.9–11.8%) to 25.0% (95%CI: 21.7–28.7). The prevalence of individuals with antibodies against the virus was higher among black and brown people, people with lower schooling and income, and among residents of regions with lower Human Development Index. The lowest prevalences were associated with recommended measures of disease protection. The proportion of asymptomatic infection was 45.1%. CONCLUSION The estimated prevalence of the infection was lower than the cumulative incidence variation, except for the last phase of the study. The differences in prevalence estimates observed among subpopulations showed social inequality as a risk of infection. The lower prevalence observed among those who could follow prevention measures reinforce the need to maintain social distancing measures as a way to prevent SARS-CoV-2 infection.

https://doi.org/10.11606/s1518-8787.2021055003970

Prevalence evolution of SARS-CoV-2 infection in the city of São Paulo, 2020–2021
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Albuquerque, José Olimpio Moura de

; Kamioka, Gabriela Akemi

; Madalosso, Geraldine

; Costa, Selma Anequini

; Ferreira, Paula Bisordi

; Pino, Francisco Alberto

; Sato, Ana Paula Sayuri

; Carvalho, Ana Carolina Aguiar de

; Amorim, Ana Beatriz Pagliaro

; Aires, Caroline Cotrim

; Kataoka, Ana Paula Arruda Geraldes

; Savani, Elisa San Martin Mouriz

; Bessa, Thirsa Alvares Franco

; Aguiar, Breno Souza de

; Failla, Marcelo Antunes

; Santos, Edson Aparecido dos

; Brito, Edjane Maria Torreão

; Santos, Maria Cristina Honório dos

; Silva, Solange Maria Saboia e

; Caldeira, Luiz Artur Vieira

; Zamarco, Luiz Carlos

; Fonseca, Sandra Maria Sabino

; Lima, Marcia Maria de Cerqueira

; Marques, Ivanilda Argenau

; Silva, Fabiana Érica Vilanova da

; Glasser, Paula Regina

; Burihan, Patrícia Carla Piragibe Ramos

; Cavazzana, Cinthya Luzia

; Mello, Debora Silva de

; Pellini, Alessandra Cristina Guedes

; Nishio, Fernando Yoshiki

; Kian, Fernanda Miyashiro

; Braga, Elza de Santana

; Bertelli, Nilza Maria Piassi

; Fracini, Wagner

; Gonçalves, Marcelo Dell Áquila

; Zular, Paulete Secco

; Piva, Regiane de Santana

; Masi, Eduardo

Revista de Saúde Pública

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2021, Volume 55 elocation 62

Abstract: En | Text: En | PDF: En

https://doi.org/10.11606/s1518-8787.2021055003970 43 downloads

Cross-sectional survey for toxoplasmosis in free-range chickens (Gallus gallus domesticus) from the Atlantic Forest area in Alagoas state, Northeastern Brazil
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Braz, Beatriz Maria de Almeida

; Feitosa, Bruna Catarina de Oliveira

; Romão, Elton Amorim

; Silva, Edson Moura da

; Pinheiro Júnior, José Wilton

; Mota, Rinaldo Aparecido

; Azevedo, Sérgio Santos de

; Porto, Wagnner José Nascimento

Revista Brasileira de Parasitologia Veterinária

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2020, Volume 29 N. 4 elocation e014120

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A toxoplasmose é uma doença parasitária mundial com um forte componente ambiental em sua cadeia de transmissão. Nesta pesquisa, foram determinados a soroprevalência e os fatores associados em galinhas caipiras de uma área de Mata Atlântica no Nordeste do Brasil. Um total de 550 galinhas caipiras foi testado para anticorpos anti-Toxoplasma gondii pela reação de imunfluorescência indireta (RIFI) com ponto de corte de 1:16, e 269 (48,9%; IC95% = 44,7- 53,1%) foram positivas. Das 61 propriedades visitadas, 57 (93,4%) apresentaram pelo menos um animal positivo. O modelo de regressão de Poisson robusta mostrou que as variáveis/categorias associadas à soroprevalência de T. gondii foram: origem dos animais/área urbana (razão de prevalência - RP = 2,346; P<0,001), sistema de manejo/uso de gaiolas (RP = 1,591; P = 0,019), presença de roedores/sim (RP = 1,295; P = 0,035), e tipo de alimento/uso de sobras de alimentos (RP = 1,603; P = 0,009). A alta prevalência sugereque este cenário demanda cuidados de manejo, principalmente no que se refere ao fornecimento de sobras de alimentos mal cozidos ou crus, higiene adequada do meio ambiente e controle frequente de roedores.

Abstract Toxoplasmosis is a parasitic disease, which is prevalent across the world and has a strong environmental component in its transmission chain. The present study determined the seroprevalence and factors related to Toxoplasma gondii infection among free-range chickens from an Atlantic Forest area in Northeastern Brazil. A total of 550 free-range chickens were tested for T. gondii antibodies usingthe indirect fluorescent antibody test (IFAT, cut-off point 1:16), and 269 (48.9%; 95% CI = 44.7-53.1%) were positive. Among the 61 properties 57 (93.4%) had at least one seropositive animal. The robust Poisson regression model showed that the variables/categories associated with the seroprevalence of T. gondii were: region of origin of the animals/urban area (prevalence ratio [PR] = 2.346; P<0.001), management system/use of cages (PR = 1.591; P = 0.019), presence of rodents/yes (PR = 1.295; P = 0.035), and the type of food/use of food scraps (PR = 1.603; P = 0.009). The high prevalence found suggest that this scenario demands careful management, mainly regarding the use of scraps of undercooked or raw food, adequate environmental hygiene and frequent rodentcontrol.

https://doi.org/10.1590/s1984-29612020087 649 downloads

Tempo de espera e absenteísmo na atenção especializada: um desafio para os sistemas universais de saúde
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Farias, Cynthia Moura Louzada

; Giovanella, Ligia

; Oliveira, Adauto Emmerich

; Santos Neto, Edson Theodoro dos

Saúde em Debate

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dec 2019, Volume 43 N. spe5 Pages 190 - 204

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo do estudo foi analisar o absenteísmo em relação ao tempo de espera por consultas e exames especializados nos 20 municípios que compõem a Região de Saúde Metropolitana do estado do Espírito Santo (RSM-ES), Brasil. Estudo descritivo retrospectivo realizado a partir da análise do Banco de Dados da Secretaria de Estado da Saúde do Espírito Santo (Sesa). Foram considerados 1.002.719 encaminhamentos dos usuários residentes na RSM-ES para consultas/exames especializados fora do município no período de janeiro de 2014 a dezembro de 2016, que correspondem a todos os agendamentos. O tempo médio de espera pela consulta foi de 419 dias (desvio padrão = 29,3, mediana = 17,0) em 2014, de 687 dias (desvio padrão = 70,5, mediana = 16,0) em 2015, de 1.077 dias (desvio padrão = 140,3, mediana = 20,0) em 2016, aumento progressivo da espera com o passar dos anos. As análises de correlação do estudo apontaram que o tempo de espera e o porte municipal são fatores correlacionados às taxas de absenteísmo em consultas e exames especializados (p-valor<5%). O impacto do absenteísmo nos serviços ambulatoriais, influenciado pelo tempo de espera, constitui-se em um grande desafio para a estruturação de um sistema público de saúde no Brasil. Conhecer como certos fatores impactam o comportamento de não comparecimento a compromissos agendados em municípios pode subsidiar mudanças nas políticas de agendamento de consultas/exames especializados.

ABSTRACT The study aimed to analyze the waiting time for specialized consultations and examinations in the twenty municipalities that compose the Metropolitan Health Region of Espírito Santo State (RSM-ES), Brazil. It is a retrospective and descriptive study was carried out based on the analysis of the Database of the State Health Department of Espírito Santo (Sesa). A total of 1,002,719 referrals from users residing in the RSM-ES were considered for specialized consultations/exams outside the municipality from January 2014 to December 2016, which corresponds to all schedulings. The average waiting time for the consultation was 419 days (standard deviation = 29.34, median = 17.00) in 2014, 687 days (standard deviation = 70.51, median = 16.00) in 2015, 1077 days (standard deviation = 140.26, median = 20.00) in 2016, progressive increase in waiting over the years. The correlation analyses of the study indicate that waiting time and municipal size are factors that can influence absenteeism in specialized consultations and examinations. The impact of absenteeism on outpatient services, influenced by waiting time, constitutes a major challenge for the structuring of a public health system in Brazil. Knowing how certain factors impact the behavior of non-attendance to appointments scheduled in municipalities can substantiate changes in scheduling policies for specialized consultations/examinations.

https://doi.org/10.1590/0103-11042019s516

10.

Effects of nitrogen fertilization and seasons on the morphogenetic and structural characteristics of Piatã (Brachiaria brizantha) grass
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Farias, Lorraine do Nascimento

; Zanine, Anderson de Moura

; Ferreira, Daniele de Jesus

; Ribeiro, Marinaldo Divino

; Lima de Souza, Alexandre

; Valério Geron, Luiz Juliano

; Araujo Pinho, Ricardo Martins

; Santos, Edson Mauro

Revista de la Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo

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dec 2019, Volume 51 N. 2 Pages 42 - 54

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The objective of this study was to evaluate the morphogenetic and structural characteristics of Piatã grass under rotational stocking and nitrogen fertilization during the seasons. A randomized complete block design in a split-plot arrangement with three replications was used. The main plots were the applications of 0, 150, 300 and 450 kg per ha of N in the form of urea, and the subplots were seasons of the year: late summer/fall, winter, spring and summer. No interaction was detected between nitrogen fertilization and season for leaf appearance rate, leaf lifespan, number of live leaves and final length leaves. However, an interaction (P<0.05) of nitrogen fertilization and season influenced leaf elongation rate, phyllochron, leaf senescence rate and stem elongation rate. The leaf elongation rate and leaf appearance rate were linearly affected (P<0.05) by nitrogen fertilization. The seasons affected (P<0.05) the leaf lifespan and number of life leaves. The leaf lifespan decreased by 0.06 days for each kg of N applied. On the other hand, the number of live leaves increased by 0.0026 leaves/tiller for each kg of N. Fertilization with nitrogen positively affects morphogenetic and structural characteristics of Piatã grass under rotational stocking. This effect can be optimized during rainy periods in spring and late summer/autumn.

El objetivo de este estudio fue evaluar las características morfogenéticas y estructurales del pasto Piatã bajo pastoreo rotacional y fertilización nitrogenada durante las diferentes estaciones del año. Se utilizó un diseño de bloques completos al azar con un arreglo de parcelas divididas con tres repeticiones. Las parcelas principales fueron las aplicaciones de 0, 150, 300 y 450 kg/ha de N en forma de urea, y las subparcelas fueron las estaciones del año: finales de verano/otoño, invierno, primavera y verano. No se detectó interacción entre la fertilización nitrogenada y la estación para la tasa de aparición de hojas, la esperanza de vida de la hoja, el número de hojas vivas y longitud final de las hojas. Sin embargo, una interacción (P<0,05) de la fertilización nitrogenada y la temporada influyeron en la tasa de elongación de la hoja, filocrono, tasa de senescencia foliar y tasa de elongación del tallo. La tasa de elongación de la hoja y la tasa de aparición de la hoja se vieron afectadas linealmente (P<0,05) por la fertilización nitrogenada. Se observó un efecto (P<0,05) de las estaciones sobre la vida media foliar y el número de hojas vivas. La vida útil de la hoja disminuyó en 0,06 días por cada kg de N aplicado. Por otro lado, el número de hojas vivas aumentó en 0,0026 hoja/macollo por cada kg de N. La fertilización con nitrógeno influye positivamente en las características morfogenéticas y estructurales del pasto Piatã bajo pastoreo rotativo. Este efecto se puede optimizar durante los períodos de lluvia, en la primavera y al final del verano/otoño.

177 downloads

11.

New initiatives for Brazilian aquatic plant data management
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Pivari, Marco Otávio Dias

; Melo, Pablo Hendrigo Alves de

; Souza, Filipe Soares

; Stehmann, João Renato

; Moura Júnior, Edson Gomes de

; Moreira, Suzana Neves

; Pott, Vali Joana

; Pott, Arnildo

; Lopes, Aline

; Moço, Maria Cecilia Chiara

; Oliveira, Lukiel dos Santos

; Lins, Alba Lucia de Almeida

; Arruda, Rafael

; Morais, Isa Lucia de

; Silva, Gustavo Santos

; Ferreira, Régis Moreira

Acta Botanica Brasilica

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mar 2019, Volume 33 N. 1 Pages 78 - 87

Abstract: En | Text: En | PDF: En

ABSTRACT Initiatives directed at managing information on aquatic plants can support different areas of scientific research, educational practices and political decisions for sustainable management and protection of wetlands and natural resources, as well as reveal knowledge gaps regarding aquatic plants and wetlands. Furthermore, such initiatives can contribute directly to consolidating wetland classification systems in Brazil. The objectives of this work are to present and explore the use of a data repository and online platform created specifically for the management of data on aquatic plants in Brazil. The data repository for aquatic plants was developed to store information on species occurrence records in a standardized form considering: systematics, taxonomy, habit, life form, geographic distribution, hydrographic basin, wetland, bibliographic references and herbarium material. A total of 3,903 records referring to the Southeast Region of Brazil were generated for the data repository. This total encompassed 1,036 species distributed among 132 families and 466 genera. Our results illustrate the diversity of information available regarding aquatic plants, circumscribe this group of species and serve as a source of data about them. Similar databases and electronic infrastructures can be developed for other flora in the future.

https://doi.org/10.1590/0102-33062018abb0280 1306 downloads

12.

Tempo de espera e absenteísmo na atenção especializada: um desafio para os sistemas universais de saúde
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Farias, Cynthia Moura Louzada

; Giovanella, Ligia

; Oliveira, Adauto Emmerich

; Santos Neto, Edson Theodoro dos

Saúde em Debate

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2019, Volume 43 N. spe5 Pages 190 - 204

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/0103-11042019s516 251 downloads

13.

TOLERANCE OF F 2 POPULATIONS OF COWPEA TO WATER DEFICIT
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RODRIGUES, ERINA VITÓRIO

; DAMASCENO-SILVA, KAESEL JACKSON

; ROCHA, MAURISRAEL DE MOURA

; BASTOS, EDSON ALVES

; SANTOS, ADRIANO DOS

Revista Caatinga

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mar 2018, Volume 31 N. 1 Pages 48 - 55

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A deficiência hídrica provoca alterações no desenvolvimento vegetal cuja reversibilidade depende do genótipo, da duração do estresse, da severidade do dano causado e do estádio de desenvolvimento em que a planta sofreu estresse. O feijão-caupi é considerado tolerante ao déficit hídrico, no entanto, estudos demonstraram que seu rendimento pode ser reduzido sob essa condição. O objetivo deste trabalho foi avaliar o efeito do déficit hídrico em populações F2 de feijão-caupi e selecionar genótipos tolerantes. Foram conduzidos dois ensaios para a avaliação das 30 populações F2, juntamente com seus genitores (1-BRS Paraguaçu, 2-Pingo de Ouro-1-2, 3-BRS Xiquexique, 4-CNCx 698-128G, 5-Santo Inácio e 6-MNC99-510F-16-1), sendo um sob déficit hídrico, imposto e outro sob irrigação plena. Os experimentos foram conduzidos no campo experimental da Embrapa Meio-Norte, em Teresina, PI. Utilizou-se o delineamento experimental látice triplo. A parcela experimental foi constituída de seis linhas de 2m, com espaçamento de 1,0 m entre linhas e 0,50 m entre plantas. Avaliou-se a produção de grãos e foi calculado o percentual de redução na produção de grãos associado a índices de produção relativa e a tolerância ao estresse. O déficit hídrico, na média, reduziu em 29,83% a produção de grãos dos genótipos. As populações que apresentaram alta produção e alta tolerância ao déficit hídrico foram: BRS Paraguaçu x CNCx 698-128G, BRS Xiquexique x Pingo de Ouro-1-2, CNCx 698-128G x BRS Xiquexique, CNCx 698-128G x MNC99-510F-16-1, Santo Inácio x BRS Xiquexique e MNC99-510F-16-1 x BRS Paraguaçu.

ABSTRACT Water deficit affects plant development and the overcoming of its effects depends on the genotype, duration of stress, severity of damage and development stage of the plant at water stress. Cowpea is considered tolerant to water deficit, however, studies have shown that its yield can be reduced under this condition. Thus, the objective of this work was to evaluate the effect of water deficit on F2 populations of cowpea and select tolerant genotypes. Thirty F2 populations of cowpea and their parents (BRS-Paraguaçu, Pingo-de-Ouro-1-2, BRS-Xiquexique, CNCx-698-128G, Santo-Inácio and MNC99-510F-16-1) were evaluated in two experiments, one under induced water deficit and other under full irrigation, in the experimental field of the Embrapa Mid-North, in Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications. The experimental plots consisted of six 2-m rows, with spacing of 1.0 m between rows and 0.50 m between plants. The grain production was evaluated and its decrease related to the relative production and tolerance to stress was calculated. The grain production of the genotypes had an average decrease of 29.83% under water deficit. The genotypes from the crosses BRS-Paraguaçu x CNCx-698-128G, BRS-Xiquexique x Pingo-de-Ouro-1-2, CNCx-698-128G x BRS-Xiquexique, CNCx-698-128G x MNC99-510F-16-1, Santo-Inácio x BRS-Xiquexique and MNC99-510F-16-1 x BRS-Paraguaçu had high production and high tolerance to water deficit.

https://doi.org/10.1590/1983-21252018v31n106rc 1290 downloads

14.

DIALLEL ANALYSIS OF TOLERANCE TO DROUGHT IN COWPEA GENOTYPES
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RODRIGUES, ERINA VITÓRIO

; DAMASCENO-SILVA, KAESEL JACKSON

; ROCHA, MAURISRAEL DE MOURA

; BASTOS, EDSON ALVES

; SANTOS, ADRIANO DOS

Revista Caatinga

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mar 2018, Volume 31 N. 1 Pages 40 - 47

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A baixa adoção de tecnologias pelos agricultores e a ocorrência de estresses bióticos e abióticos são fatores que limitam a produção do feijão-caupi na região Nordeste do Brasil. A tolerância de genótipos à seca é uma das formas para diminuir os efeitos negativos dos estresses sobre a produtividade. O objetivo deste trabalho foi identificar genitores e combinações promissoras com maior probabilidade de gerarem populações segregantes de feijão-caupi tolerantes à seca. Foi realizado um dialelo completo envolvendo seis genótipos de feijão-caupi, totalizando 30 populações F2, que foram avaliadas, juntamente com seus genitores, em um ensaio sob déficit hídrico, no campo experimental da Embrapa Meio-Norte, em Teresina-PI. Utilizou-se o delineamento experimental látice triplo, com parcela de 6 linhas de 2 m, sendo amostradas 15 plantas por parcela. Foram avaliados caracteres agronômicos e submetidos à análise de variância e, utilizando-se das médias, obtiveram-se as estimativas de capacidade geral e específica de combinação. Observou-se diferenças significativas para todos os caracteres, evidenciando a existência de variabilidade genética na população. Os efeitos aditivos foram mais importantes que os efeitos não aditivos, sendo também constatada a presença de herança materna. Os genótipos BRS Xiquexique, Pingo de Ouro-1-2 e MNC99- 510F-16-1 foram os mais promissores para utilização em programa de seleção recorrente visando tolerância ao déficit hídrico. As combinações híbridas (1) BRS Paraguaçu X (4) CNCx 698-128G, (2) Pingo de Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo Inácio, (4) CNCx 698-128G X (6) MNC99-510F-16-1 e (5) Santo Inácio X (4) CNCx 698-128G, possuem potencial simultâneos para extração de linhagens superiores quanto à produtividade de grãos e tolerantes ao déficit hídrico.

ABSTRACT The low use of technologies by farmers and the occurrence of biotic and abiotic stresses are limiting factors for cowpea production in the Brazilian Northeast region. The tolerance of genotypes to drought is an alternative to decrease the negative effects of stresses on cowpea production. Thus, the objective of this work was to identify parents and combinations of cowpea genotypes with high probability of generating segregating populations with tolerance to drought. Six cowpea genotypes were crossed in a complete diallel cross design, totaling 30 F2 populations, which were evaluated together with their parents in an experiment under water deficit at the experimental field of the Embrapa Mid-North, Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications, with experimental plot consisting of six 2-m plant rows. Fifteen plants per plot were sampled to evaluate their agronomic characteristics, whose results were subjected to analysis of variance and means were used to estimate the general and specific combining abilities. The genotypes showed significant differences in all characteristics evaluated, denoting the genetic variability of the population. The additive effects were more important than the non-additive effects, and maternal inheritance was detected. The genotypes BRS Xiquexique, Pingo-de-Ouro-1-2 and MNC99-510F-16-1 were the most promising for use in recurrent selection programs for tolerance to water deficit. The hybrid combinations (1) BRS Paraguaçu X (4) CNCx-698-128G, (2) Pingo-de-Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo-Inácio, (4) CNCx-698-128G X (6) MNC99-510F-16-1 and (5) Santo-Inácio X (4) CNCx-698-128G showed potential for generating superior lineages regarding bean production and tolerance to water deficit.

https://doi.org/10.1590/1983-21252018v31n105rc 741 downloads

15.

Associação de Fungos com Sementes de Espécies Florestais Nativas
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Carmo, Ana Lídia Moura do

; Mazaratto, Edson José

; Eckstein, Bárbara

; Santos, Álvaro Figueredo dos

Summa Phytopathologica

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sep 2017, Volume 43 N. 3 Pages 246 - 247

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO No Brasil, ainda há poucas pesquisas sobre os microrganismos associados às sementes florestais. O objetivo desse trabalho foi detectar fungos associados às sementes de seis espécies florestais nativas: açoita-cavalo (Luehea divaricata), araçá amarelo (Psidium cattleyanum), corticeira (Erythrina falcata), rabo-de-bugio (Lonchocarpus sp.), vassoura-vermelha (Dodonea viscosa) e pau-jacaré (Piptadenia gonoacantha). Foram identificados oito gêneros de fungos potencialmente patogênicos: Cladosporyum sp., Fusarium sp., Pestalotiopsis sp., Phomopsis sp., Coletotrichum sp., Alternaria sp., Phoma sp. e Botrytis sp. e fungos saprófitas: Aspergillus sp., Penicillium sp., Rhizopus sp., Epicoccum sp., Torula sp., Trichoderma sp., Gliocladium sp., Chaetomium sp. e Trichothecium sp. Os resultados evidenciam a diversidade de fungos associados às sementes florestais nativas, incluindo vários potencialmente fitopatogênicos.

ABSTRACT In Brazil, there is still scarce research on microorganisms associated with forest seeds. The aim of this study was to detect fungi associated with seeds of six native forest species: “açoita-cavalo” (Luehea divaricata), “araçá amarelo” (Psidium cattleyanum), “corticeira” (Erythrina falcata), “rabo-de-bugio” (Lonchocarpus sp.), “vassoura-vermelha” (Dodonea viscosa) and “pau-jacaré” (Piptadenia gonoacantha). Eight genera of fungi were identified as potentially pathogenic: Cladosporyum sp., Fusarium sp., Pestalotiopsis sp., Phomopsis sp., Coletotrichum sp., Alternaria sp., Phoma sp. and Botrytis sp., and saprophytic fungi: Aspergillus sp., Penicillium sp., Rhizopus sp., Epicoccum sp., Torula sp., Trichoderma sp., Gliocladium sp., Chaetomium sp. and Trichothecium sp. Results evidence the diversity of fungi associated with native forest seeds, including several potentially phytopathogenic fungi.

https://doi.org/10.1590/0100-5405/2211 2186 downloads

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