Pheromones are essential for colony organization in honeybees. The pheromones 9-ODA ((E)-9-oxodec-2-enoic acid) and 9-HDA ((E)-9-hydroxydec-2-enoic acid), produced by the mandibular gland of the queen, have various functions within the colony, including inhibition of ovarian development of workers, attraction of swarms, and stabilization of the cluster group. An example of reaction of the colony to adverse conditions is absconding, which is defined as mass exit of all of the adult individuals, leaving even brood and food. In this study, the pheromones 9-ODA and 9-HDA were determined in virgin and normal mated, egg-laying European Carniolan and Africanized queens, as well as in mated queens that were in absconding colonies, using liquid chromatography with UV detection developed methodology. Absconding was induced by maintaining free-flying five-standard-Langstroth-frame colonies in a chamber artificially heated to 45 ºC. The obtained results showed that the amount of 9-ODA in Africanized queens (6.56 µg bee-1) is very low compared with European queens. However, large amounts of 9-HDA were found in the queens of absconding Africanized swarms (107.4 µg bee-1). According to our results, the quantities of 9-ODA and 9-HDA in Africanized honeybees in Brazil may be contributing to the high rates of absconding, promoting low effect of stabilizing agent, and high effect of attraction of dispersers cluster.

Abstract Introduction: Overactive bladder syndrome (OABS) affects up to 43% of women. Staggered therapy includes habit changes, drugs, and peripheral neuromodulation. Up to 40% of patients may require advanced alternative therapy such as botulinum toxin A (BT). Objective: To report the results of treatment with TB in women with OABS refractory to first- and second-line treatment in a public hospital in Chile. Method: Retrospective cohort study from prospectively collected database from Hospital Sótero del Río between 2018 and 2022. Thirty-three patients with previous pharmacological treatment and neuromodulation were included, with an average age of 57 years, undergoing cystoscopic injection of TB to detrusor. Demographic data, history, and complications were analyzed. Follow-up was performed with voiding frequency, cloths/day, Sandvik Severity Scale (ISI) and Patient Global Impression Improvement (PGI-I) survey. Results: Comparative data pre/post TB, respectively showed: daytime voiding frequency 11/6 and nighttime 5/1; cloths/day 5/3; ISI 8/3. 20 of 33 women reported being excellent or much better (PGI-I). Conclusions: TB management in women with OABS refractory to first- and second-line treatment has good results and significant impact on quality of life.

Resumen Introducción: El síndrome de vejiga hiperactiva (VHA) afecta hasta al 43% de las mujeres. La terapia escalonada incluye cambios de hábitos, fármacos y neuromodulación. Hasta el 40% de las pacientes llegan a requerir terapia avanzada alternativa, como toxina botulínica (TB). Objetivo: Reportar los resultados del tratamiento con toxina botulínica en mujeres con VHA refractaria a tratamiento de primera y segunda línea, en un hospital público en Chile. Método: Estudio de cohorte retrospectivo desde una base de datos recolectada prospectivamente del Hospital Sótero del Río entre 2018 y 2022. Se incluyeron 33 pacientes con tratamiento farmacológico y neuromodulación previa, con una edad promedio de 57 años, sometidas a inyección cistoscópica de TB a detrusor, analizando datos demográficos, antecedentes y complicaciones. Se realizó seguimiento con frecuencia miccional, paños/día, escala de gravedad de Sandvik (ISI) y encuesta Patient Global Impression Improvement (PGI-I). Resultados: Los datos comparativos pre/post TB, respectivamente, fueron: frecuencia miccional diurna 11/6 y nocturna 5/1; paños/día 5/3; ISI 8/3. Veinte de 33 mujeres refirieron estar excelente o mucho mejor (PGI-I). Conclusiones: Es posible realizar manejo con TB en mujeres con VHA refractaria a primera y segunda línea, con buenos resultados e impacto significativo en la calidad de vida.

RESUMEN: Se ha propuesto que las medidas del hueso hioides varían entre los diferentes sexos y grupos de edad. El objetivo del estudio fur estudiar la morfometría del hueso hioides en pacientes jordanos. Se analizaron tomografías computarizadas en 3D de 637 pacientes. Se midieron diez parámetros del hueso hioides, incluyendo la longitud anteroposterior, la longitud de los cuernos mayores (derecho e izquierdo), la altura de los cuernos mayores (derecho e izquierdo), el ancho del cuerpo hioides, la altura del cuerpo hioides, la distancia entre los puntos medios de los extremos posteriores de los cuernos mayores del hueso hioides, el ángulo entre los cuernos mayores derecho e izquierdo, y la distancia del hueso hioides a la columna vertebral. Además, se documentaron el nivel vertebral, el rango de fusión, la morfología de la língula del cuerpo hioides y la forma del hueso hioides. Todas las dimensiones del hioides fueron más largas en los hombres, pero el mayor ángulo de los cuernos fue más ancho en las mujeres. En pacientes menores de 30 años, los parámetros son los más pequeños con el ángulo más amplio. La distancia del hioides a la columna vertebral es mayor en el sexo masculino (30-49) años. No se observó fusión entre el cuerpo hioides y los cuernos mayores en pacientes menores de 10 años, pero se encontró fusión (unilateral o bilateral) en solo el 73,2 % de los pacientes ≥ 70 años. El hioides estaba mayormente al nivel de la vértebra C3 y en forma de “U”. La forma de la língula era mayoritariamente "Cicatriz" en los hombres (especialmente ≥ 50 años) y "Nada" en las mujeres (especialmente < 50 años). El máximo crecimiento de las dimensiones del hioides es antes de los 30 años. La fusión entre el cuerpo hioides y los cuernos mayores no se observó en pacientes menores de 10 años. No obstante, las características del hueso hioides no pudieron predecir la edad o el sexo en nuestra muestra. Además, la tomografía computarizada 3D es una herramienta excelente para evaluar la anatomía de la región de la cabeza y el cuello.

SUMMARY: Hyoid bone measurements have been proposed to vary between different genders and age groups. The aim of the study is to study hyoid morphometrics among Jordanian patients. 3D-CT scans of 637 patients were analyzed. Ten parameters of hyoid bone were measures, including the anteroposterior length, length of greater horns (right and left), height of greater horns (right and left), width of hyoid body, height of hyoid body, the distance between the midpoints of the posterior ends of the greater horns of the hyoid bone, the angle between right and left greater horns, and the distance of the hyoid bone to the vertebral column. Also, vertebral level, fusion rank, morphology of hyoid body lingula, and shape of hyoid bone were documented. All hyoid dimensions were longer in males, but greater horns angle was wider in females. In patients younger than 30 years, the parameters are the smallest with the widest angle. The distance from hyoid to vertebral column is higher in males (30-49) years of age. No fusion between hyoid body and greater horns was observed in patients younger than 10 years, but fusion (unilateral or bilateral) was found in only 73.2 % of patients ≥ 70 years. The hyoid was mostly at vertebra C3 level and “U” shaped. The lingula shape was mostly “Scar” in males (especially ≥ 50 years) and “Nothing” in females (especially < 50 years). The maximum growth of hyoid dimensions is before age of 30 years. Fusion between hyoid body and greater horns was not seen in patients younger than 10 years. Otherwise, the hyoid features failed to predict age or gender in our sample. Furthermore, 3D-CT scan is an excellent tool to assess the anatomy of head and neck region.

ABSTRACT: Toxoplasma gondii can be eliminated in bovine semen. Cryopreserved semen is often used due to the fact that artificial insemination in dairy and beef cattle provides benefits in terms of production. However, little is known regarding the viability and infectivity of T. gondii tachyzoites in cryopreserved bovine semen. In the present study, cattle semen negative for T. gondii were contaminated with 1 x 106 tachyzoites (RH strain) and cryopreserved with and without different cryoprotectants, such as DMSO (concentrations of 2.5%, 5.0%, 7.5%, 8.0% and 10.0%) and glycerol (2.25%, 2.5%, 3.0%, 5.0%, 7.5% and 10.0%), followed by freezing in liquid nitrogen (-196°C). After 24 hours, the samples were thawed and inoculated in 10 mice per cryoprotectant concentration. The mice were evaluated for clinical signs of toxoplasmosis (rough coat, diarrhea, hypoactivity and sudden death) as well as serum titers of IgM and IgG and the presence of tachyzoites in the peritoneal lavage. The results revealed that T. gondii remained infective in all samples. Clinical signs of toxoplasmosis were observed in the mice beginning with the 6th day post-inoculation (DPI) and 100% lethality was found between the 7th and 9th DPI. Viable tachyzoites were recovered from peritoneal exudate of dead mice (except for the control group), with higher mean of tachyzoite counts in the intraperitoneal lavage for 5% DMSO (±3.32 x 106), 8% DMSO (±3.53 x 106), 3% glycerol (±4.75 x 106), 7.5% glycerol (±6.26 x 106) and the absence of cryoprotectant (±3.11 x 106). Seroconversion occurred in the treated groups, with titers of IgG from 1:16 to 1:128 and IgM from 1:16 to 1:512. T. gondii viability and infectivity were maintained in cattle semen during 24 hours of cryopreservation at -196°C with and without cryoprotectant. However, further studies are necessary to determine whether cryopreserved semen contributes to the spread of toxoplasmosis through artificial insemination.

RESUMO: Sabe-se que Toxoplasma gondii pode ser eliminado no sêmen bovino. A inseminação artificial em bovinos leiteiros e de corte proporcionou avanços e benefícios nas produções e para isso o sêmen criopreservado é frequentemente utilizado. No entanto, pouco se sabe sobre a viabilidade e infectividade dos taquizoítos de T. gondii em sêmen bovino criopreservado. Para isso o sêmen bovino, negativo para T. gondii, foi contaminado com 1x106 taquizoítos (cepa RH), criopreservados com ou sem diferentes crioprotetores como DMSO (2.5%, 5.0%, 7.5%, 8.0% e 10.0%) e Glicerol (2.25%, 2.5%, 3.0%, 5.0%, 7.5% e 10.0%) e congelados em nitrogênio líquido (-196°C). Após 24 horas, essas amostras foram descongeladas e inoculadas em 10 camundongos por diluente de concentração de crioprotetor. Os camundongos foram avaliados quanto a sinais clínicos de toxoplasmose (pele áspera, diarreia, hipoatividade e morte súbita), títulos séricos de IgM e IgG e presença de taquizoítos no lavado peritoneal. Os resultados mostraram que T. gondii se manteve infectante em todas as amostras, inclusive naquelas sem crioprotetor. Sinais clínicos de toxoplasmose foram observados nos camundongos a partir do 6º dia pós-inoculação (DPI) e 100% de letalidade foi verificada entre o 7º ao 9º DPI. Nos camundongos mortos, exceto no grupo controle, taquizoítos viáveis foram recuperados do exsudato peritoneal, com maior média de taquizoítos quantificados na lavagem intraperitoneal para DMSO a 5% (±3.32x106), 8% (±3.53x106) e glicerol 3% (±4.75x106), 7,5% (±6.26x106) e livre de crioprotetor (±3.11x106). A soroconversão ocorreu nos grupos tratados com títulos de IgG (1:16 a 1:128) e IgM (1:16 a 1:512). A viabilidade e infectividade do T. gondii no sêmen bovino durante as 24 horas de criopreservação a -196°C foram mantidas com ou sem crioprotetor. No entanto, mais estudos são necessários para verificar se o sêmen criopreservado contribui para a disseminação da toxoplasmose na inseminação artificial.

Abstract The present study aimed was to evaluate the spatial variability of weed species by means of phytosociological parameters and their correlations with the physical-chemical soil properties, under semiarid climate conditions. Weed phytosociology and soil characterization were carried out in two areas one newly deforested area covering 8.86 ha, and one experimental agricultural area covering 24.7 ha; both in the semi-arid region of Brazil. Weed and soil were sampled by following georeferenced grids in each area. Biomass and the total number of weed individuals, as well as soil properties, were mapped by the ordinary Kriging method. The predominant herbaceous plants in the newly deforested area were Hexasepalum teres and Digitaria insularis. The weed species that predominated in the agricultural area were Cyperus rotundus L., Euphorbia heterophylla L. and Herissantia Crispa (L.) Brizicky; the latter species outstanding for dry biomass (873.5g). Spatial dependence was observed for the predominant species, except for Digitaria insularis. The spatial distribution of these weeds was conditioned by soil K+ contents in both areas, and by sand content for the experimental agricultural area. Therefore, these two soil attributes resulted key factors for weed infestation in this semi-arid region.

Resumen Entre las duras estaciones e inclemencias del tiempo, olvidados y desprovistos de docentes, transporte, infraestructura moderna y materiales educativos, llegar a los campo-escuelas de la sierra puede tardar horas de empinada caminata para niños de primaria y pequeños infantes, en el que las dificultades propias de espacios rurales y la dureza de la altura puede no ser el mayor desafío de todos. Pues, sin privilegio alguno, cruzando cerros y montañas, son niños y niñas que salen de la nada para conquistarlo todo. Desde esta premisa, el estudio propone engrosar la relación entre Escuela-Montaña (EM) a manera de forjar no solamente una renovada noción o línea real que permita responder a los actuales dilemas y retos de la época, sino que, además, permita encontrar algunos motores de acción fundidas y forjadas desde el corazón de la montaña, una que tenga la suficiente capacidad y el espíritu necesario de asesinar al asesino. De esta forma, en línea de estudios que plantean degollar al emperador o de provocar un atípico evento telúrico en las regiones del poder, este estudio deposita su alma y espíritu dentro de trabajos parametrados por la investigación documental en el marco de las normas preestablecidas por la metodología PRISMA. De los resultados puede expresarse que, en ese lugar de glaciares y nevados destinados a desaparecer con el pasar de los años, los pobladores de altura no solo carecen de múltiples atenciones, sino que además de estar en la intemperie, su aparatosa y brutal embestida tiene el impacto de fuertes desventuras, donde la escuela, como nunca antes rige sus propios desafíos.

Abstract Between the harsh seasons and inclement weather, forgotten and devoid of teachers, transportation, modern infrastructure and educational materials, reaching the field-schools of the mountains can take hours of steep walking for primary school children and small infants, in which the difficulties of rural areas and the hardness of the height may not be the greatest challenge of all. Well, without any privilege, crossing hills and mountains, they are boys and girls who come out of nowhere to conquer everything. From this premise, the study proposes to enlarge the relationship between School-Mountain (MS) in order to forge not only a renewed notion or real line that allows responding to the current dilemmas and challenges of the time, but also allows finding some action engines cast and forged from the heart of the mountain, one that has the ability and spirit to slay the killer. In this way, in line with studies that propose beheading the emperor or provoking an atypical telluric event in the regions of power, this study deposits its soul and spirit within works parameterized by documentary research within the framework of the norms pre-established by the PRISMA methodology. From the results it can be stated that, in that place of glaciers and snow-capped mountains destined to disappear over the years, the high-altitude inhabitants not only lack multiple attentions, but in addition to being out in the open, their cumbersome and brutal onslaught has the impact of strong misadventures, where the school, like never before governs its own challenges.

RESUMO: O artigo apresenta em detalhe a gênese do Fundo Monteiro Lobato, no CEDAE/Unicamp, abrangendo desde o projeto inicial até o desenvolvimento do Acervo ao longo dos anos. Algumas seções são mais explicitadas, como cartas (algumas transcritas), fotografias, livros e presença na mídia. Destaca seu papel para o conhecimento mais completo das plateias e bastidores da história literária brasileira dos inícios do século XX, para além dos estereótipos correntes sobre o escritor.

ABSTRACT: The article presents in detail the genesis of the Monteiro Lobato Fund, at CEDAE/Unicamp, covering from the initial project to the development of the Archive over the years. Some sections are more explicit, such as letters (some transcribed), photographs, books and presence in the media. It highlights its role for a more complete knowledge of the audiences and backstage of Brazilian literary history of the beginning of the 20th century, beyond the current stereotypes about the writer.

RESUMO A serapilheira é uma importante fonte de nutrientes para o solo e a sua decomposição pode ser influenciada pelos sistemas de uso do mesmo. Este estudo teve por objetivo avaliar a decomposição de serapilheira e seu estoque de macronutrientes em dois sistemas de cultivo do cafeeiro, sendo um sombreado (CS) e outro a pleno sol (CPS). O experimento foi realizado em uma propriedade rural do município de Cacoal, Estado de Rondônia, Brasil, em esquema fatorial 2 × 6, dois usos do solo (CS e CPS) e seis épocas de avaliação da decomposição da serapilheira (0, 30, 60, 180, 300 e 360 dias), com sete repetições. A decomposição de serapilheira foi avaliada aos 0, 30, 60, 180, 300 e 360 dias e, além disso, foi determinada a constante de decomposição (k) e o tempo de meia vida (t1/2) aos 360 dias e os teores de fósforo (P), potássio (K), cálcio (Ca), magnésio (Mg), enxofre (S) e nitrogênio (N) da serapilheira remanescente em cada tempo de decomposição. No CS há maiores perda de massa e constante de decomposição, e menor tempo de meia vida da serapilheira ao final do período. A serapilheira apresenta perda de massa em função do tempo de decomposição. Os estoques de macronutrientes N, P, K, Ca e Mg reduziram-se de forma linear durante o tempo de decomposição e ocorreu incremento no estoque de enxofre ao final do período.

ABSTRACT Litterfall is an important source of soil nutrients, but its decomposition can be affected by the crop system used. The objective of this study was to evaluate litterfall decomposition and macronutrient stocks in coffee crop systems in shaded (SHCS) environments and those in full sun (FSCS). The experiment was conducted on a rural property in Cacoal, state of Rondônia, Brazil, in a 2 × 6 factorial scheme with two crop systems (SHCS and FSCS), and six litterfall decomposition evaluation times (0, 30, 60, 180, 300, and 360 days after the litterfall was returned to the soil (DAL)), with seven replicates. The constant of decomposition (k), half-life time (t1/2) at 360 DAL, and phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), and nitrogen (N) concentrations of the remaining litterfall were determined at each evaluation time. The litterfall in the SHCS had a greater weight loss and constant of decomposition and a lower half-life time at the last evaluation, and the weight loss increased as a function of decomposition time. The litterfall stocks of macronutrients N, P, K, Ca, and Mg showed a linear decrease throughout the decomposition time, and increases in sulfur stock were found at the last evaluation.

Ambuic acid (AA) is a highly-modified cyclohexenone and known as a promising inhibitor of quorum sensing in methicillin-resistant Staphylococcus aureus, and is thus a candidate as an antivirulence drug. This molecule is mainly produced by the species of Pestalotiopsis and, since its discovery twenty years ago, only a restricted amount of AA-derivatives have been described. Despite being a promising subject, methods for the analysis of modified AA-analogues via mass spectrometry remain unexplored. In order to adress this question, the marine fungus Pestalotiopsis sp. 4A11 associated with the ascidian Didemnum perlucidum was grown in a solid rice medium and its crude extract was chemically studied. From this extract, AA and 10-hydroxy ambuic acid (10-HAA) were isolated and identified using NMR spectroscopy with the aim of obtaining model compounds for the MS analysis. These served as reference compounds (seeds) to guide the dereplication of other AA-analogues via LC-MS/MS-based molecular networking. Based on the manual interpretation of the fragmentation pathways of the seeds and related compounds observed in the networks, six AA-derivatives were dereplicated in the extract. Furthermore, three analogues with unprecedented chemical formulas were proposed as putative unprecedented AA-derivatives. The fragmentation annotation proposed represents a fast and feasible method for characterizing AA-derivatives.

Bio-based linear low-density polyethylene (green LLDPE) composites are used as electrical jackets/insulator for cables. Assessments of gamma-irradiation (Co-60) effects on these materials are of interest as they might be used in nuclear power plants (NPPs). Brazilian sugarcane juice-based green LLDPE composite electrical jackets were irradiated until 1000 kGy and analyzed for thermal stability and mechanical characteristics. Thermogravimetry analysis (TGA) showed increasing of pyrolysis activation energy (Ea) (under N2) from 42.7 ± 4.2 kJ/mol in unirradiated samples to 72.8 ± 4.6 kJ/mol after 60 kGy dose, as resulted of radiation-induced effects. FTIR spectra evidenced radiation-induced formation of conjugated C=C bonds after 250 kGy dose. Tensile stress and Young modulus did not change significantly until 150 kGy dose, whereas elongation at break decreased and reached 50% at 91 kGy dose. These results suggest that green LLDPE might withstand radiation damage through a NPP operating life (~ 40 years).

Abstract UVB-irradiation increases the risk of various skin disorders, therefore leading to inflammation and oxidative stress. In this sense, antioxidant-rich herbs such as Rosmarinus officinalis may be useful in minimizing the damage promoted by reactive oxygen species. In this work, we report the efficacy of a R. officinalis hydroethanolic extract (ROe)-loaded emulgel in preventing UVB-related skin damage. Total phenols were determined using Folin-Ciocalteu assay, and the main phytocomponents in the extract were identified by UHPLC-HRMS. Moreover, in vitro sun protection factor (SPF) value of ROe was also assessed, and we investigated the in vivo protective effect of an emulgel containing ROe against UVB-induced damage in an animal model. The ROe exhibited commercially viable SPF activity (7.56 ± 0.16) and remarkable polyphenolic content (24.15 ± 0.11 mg (Eq.GA)/g). HPLC-MS and UHPLC-HRMS results showcased that the main compounds in ROe were: rosmarinic acid, carnosic acid and carnosol. The evaluation of the in vitro antioxidant activity demonstrated a dose-dependent effect of ROe against several radicals and the capacity to reduce iron. Therefore, we demonstrated that topical application of the formulation containing ROe inhibited edema formation, myeloperoxidase activity, GSH depletion and maintained ferric reducing (FRAP) and ABTS scavenging abilities of the skin after UVB exposure.

Objective: The aim of the present study was to analyze the body sway response in specific phobia (SP) patients and healthy controls while viewing neutral, phobic, and disgusting images. Methods: The participants’ heart rate (HR) and skin conductance were also recorded during the procedure. Nineteen patients with arachnophobia and 19 healthy volunteers matched by age, gender, and years of education underwent a postural control test on a stabilometric platform. Results: The platform recorded increased body sway in the SP group when exposed to spider images (SPI). The SP group presented increases in most parameters (SD, velocity, frequency, area, p ≤ 0.05) when viewing pictures of the SPI category. Psychometric measures of subjective anxiety (State-Trait Anxiety Inventory, STAI) and physiological states (HR; skin conductance responses; spontaneous fluctuations in skin conductance) showed increased anxiety (p ≤ 0.05) in the SP group compared to healthy volunteers. High anxiety levels were observed throughout the assessment, including the task of exposure to SPI (p ≤ 0.05). No significant effect or correlation was found between skin conductance and body sway measures (p > 0.05). Conclusions: The results of the postural control test suggest the occurrence of a defensive escape response in SP, in agreement with previous evidence.

Selecting a polymer depends on its characteristics, the properties of the drug and of the remaining ingredients in the formulation. The drug, when incorporated into a polymeric matrix, can show several advantages when compared with its conventional form. In this context, this work describes the preparation and characterization of polyethylene glycol (PEG 4000) and its derivative particles loaded with benznidazole, as well as evaluates their trypanocidal activity. In this work, reactions to modify the PEG 4000 polymer and the subsequent incorporation of the benznidazole were made. The nuclear magnetic resonance (NMR) analysis confirmed the efficiency in modifying the PEG chains. The morphology of polymeric films was observed by atomic force microscopy (AFM) and showed considerable changes on the film organization. The acetylation of PEG favored the stability of the system and an increase in the zeta potential from ?14.83 to ?25.54 mV was observed. Although encapsulation efficiency values between 30.14 and 39.48% were found, the enhanced benznidazole dissolution profile by microparticles enables the use of lower drug concentrations. This fact can be proven by the increased trypanocidal effect of benznidazole when encapsulated in BP3 microparticles. Finally, the high selectivity of the formulations for trypanocidal action guarantees their safety as an alternative for the treatment of the Chagas disease.

RESUMO Este artigo objetiva adaptar transculturalmente um questionário de qualidade de vida para individuos com doenças orais potencialmente malignas para o contexto brasileiro. Este estudo metodológico consistiu nas seguintes etapas do processo de validaçâo de conteúdo: (1) Equivalência conceitual e de itens - etapa em que foi realizada uma ampla revisao da literatura sobre o construto; (2) Equivalência semántica. A extensa revisao da literatura mostrou que o questionário permite avaliar a QV e que os dominios e itens também sao considerados e relevantes para o contexto brasileiro. A equivalência semántica foi avaliada pelo comité de juízes de forma satisfatória. A análise do escopo dos dominios foi realizada pela taxa de concordância e apresentou resultados iguais ou superiores a 84%. Para a análise de clareza e representatividade, o resultado do cálculo geral do Cálculo de Validade de Conteúdo (CVC) foi de 0,84 e 0,92 respectivamente. Dos 20 itens, 18 apresentaram valores de IVC acima de 0,8. Os indicadores de validaçâo de conteúdo, pré-teste e equivalência operacional indicam que a versâo brasileira do QoL-OPMD é um instrumento promissor e que parece válido para avaliar a qualidade de vida de pessoas com doenças orais potencialmente malignas. Como próximo passo, é importante avaliar as propriedades psicométricas desse instrumento.

ABSTRACT The aim ofthis article is to describe the cross-cultural adaptation of a quality of life (QoL) questionnaire for individuals with potentially malignant oral diseases (OPMD) in the Brazilian context. This methodological study consisted of the following stages of content validation process: (1) Conceptual and item equivalence: stage during which a comprehensive literature review on the construct was performed; (2) Semantic equivalence. The extensive literature review showed that the questionnaire enables evaluation of QoL, and that domains and items are also considered and relevant to the Brazilian context. Semantic equivalence was evaluated as satisfactory by a committee of judges. The scope of the domains was analyzed according to the agreement rate and presented results equal to or greater than 84%. The general Content Validity Calculation (CVC) was 0.84 for clarity and 0.92 for representativeness. Of the 20 items, 18 presented CVC values above 0.8. The indicator for content validation, pre-test and operational equivalence indicate that the Brazilian version of the OPMD QoL questionnaire is a promising instrument and a tool that seems valid to evaluate the quality of life of people with oral potentially malignant disorders. As a next step, it is important to measure equivalences to evaluate the psychometric properties of this instrument.

RESUMO Objetivos: Determinar a associação entre a necessidade de tratamento ortodôntico e o bullying autorrelatado e seu impacto na autoestima dos adolescentes. Métodos: A amostra foi composta por 160 escolares de 11 a 14 anos, matriculados em escolas públicas. A história de bullying foi avaliada por meio do Questionário Crozier; adaptado por Dimmock (1999). A autoestima foi determinada por meio do Global Negative Self-Evaluation Scale (GSE). A necessidade normativa de tratamento ortodôntico foi determinada pelo Componente de Saúde Bucal (DHC) e a necessidade percebida, pelo Componente Estético (AC) do Índice de Necessidade de Tratamento Ortodôntico (IOTN). Foram realizadas análises individuais das associações com a história de bullying (desfecho). Resultados: As variáveis que apresentaram P ? 0.20 nas análises individuais foram testadas em modelo de regressão logística múltipla, permanecendo no modelo as variáveis com P ? 0.05. Foram estimados os odds ratios com os intervalos de confiança. De acordo com os resultados, 72.5% dos escolares relataram história de bullying. Os escolares que apresentaram impacto negativo na qualidade de vida relacionado aos sintomas bucais e autoestima negativa tiveram 4.59 e 5.44 vezes mais chance, respectivamente, de relatarem história de bullying (P < 0.05), e não sofreram influência da necessidade de tratamento ortodôntico normativa e percebida. Conclusão: A história de bullying não foi influenciada pela necessidade de tratamento ortodôntico, mas pela baixa autoestima dos adolescentes.

ABSTRACT Objective: To determine the association between orthodontic treatment need and the self-reported bullying, and its impact on adolescents’ self-esteem. Methods: The sample consisted of 160 schoolchildren from 11 to 14 years of age, enrolled in public schools. The history of bullying was evaluated by means of the Crozier; Dimmock adapted Questionnaire (1999). Self-esteem was determined by means of the Global Negative Self-Evaluation Scale (GSE). The normative orthodontic treatment need was determined by the Dental Health Component (DHC) and perceived need, by Aesthetic Component (AC) of the Index of Orthodontic Treatment Need (IOTN). Individual analyses were made of the associations with the history of bullying (outcome). Results: The variables that presented P ? 0.20 in the individual analyses were tested in a multiple logistic regression model, and variables with P ? 0.05 remained in the model. The odds ratios with the intervals of confidence were estimated. According to the results 72.5% of the schoolchildren reported a history of bullying. The schoolchildren who presented negative impact on the quality of life related to oral symptoms and negative self-esteem were observed to have 4.59 and 5.44 times more chance, respectively to report a history of bullying (P < 0.05), which did not suffer the influence of normative and perceived orthodontic treatment need. Conclusion: The history of bullying was not influenced by orthodontic treatment need, but by the low self-esteem of adolescents.