Abstract The Guarani (GAS) and Serra Geral (SGAS) aquifer systems are the main groundwater sources in southern Brazil. We investigated the crucial relationship between the primary, intergranular pore system in the GAS and the secondary pore system associated with fractures in the SGAS in order to understand better the compartmentalization and connection, water storage, and productivity of both aquifers. Data from 375 wells that produce from both aquifers was used to evaluate the hydrodynamic characteristics of the GAS-SGAS contact. The integration of data using remote sensing, structural data, and thin section analysis support interpretations about the compartmentalization and potential connection of the aquifers. Fault zones trending NW, EW, and NNE demarcate six structural blocks in which the GAS-SGAS contact varies more than 400 m in elevation. Thin section analysis of sandstone texture and composition allowed us to determine the relationship between the type of porosity and cement type and content. The surface demarcating the Guarani-Serra Geral contact is highly productive in 93% of the wells that reach both aquifers. The integration of our results allowed us to propose a conceptual hydrogeological model for the GAS and SGAS aquifers in the study area.

The complete genome sequence of bovine papillomavirus 2 (BPV2) from Brazilian Amazon Region was determined using multiple-primed rolling circle amplification followed by Illumina sequencing. The genome is 7,947 bp long, with 45.9% GC content. It encodes seven early (E1, E2,E4, E5, E6,E7, and E8) and two late (L1 and L2) genes. The complete genome of a BPV2 can help in future studies since this BPV type is highly reported worldwide although the lack of complete genome sequences available.

Adjuvants play an important role in vaccine formulations by increasing their immunogenicity. In this study, the phenolic compound-rich J fraction (JFR) of a Brazilian green propolis methanolic extract stimulated cellular and humoral immune responses when co-administered with an inactivated vaccine against swine herpesvirus type 1 (SuHV-1). When compared to control vaccines that used aluminium hydroxide as an adjuvant, the use of 10 mg/dose of JFR significantly increased (p < 0.05) neutralizing antibody titres against SuHV-1, as well as the percentage of protected animals following SuHV-1 challenge (p < 0.01). Furthermore, addition of phenolic compounds potentiated the performance of the control vaccine, leading to increased cellular and humoral immune responses and enhanced protection of animals after SuHV-1 challenge (p < 0.05). Prenylated compounds such as Artepillin C that are found in large quantities in JFR are likely to be the substances that are responsible for the adjuvant activity.