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Resumo As comunidades quilombolas, estão presentes em diversos estados brasileiros, vivendo em condições de saúde mais precárias. Isto ocorre por conta do isolamento geográfico, das limitações de acesso e da falta de qualidade no serviço quando este é prestado. Nesse sentido, o objetivo do estudo foi analisar a qualidade de vida de mulheres de uma comunidade quilombola do nordeste brasileiro. Estudo observacional, transversal e descritivo. Foram entrevistadas 160 mulheres adultas, através de um formulário para a coleta de perfil e do questionário de Qualidade de Vida WHOQOL – bref. Foi observado que as mulheres tinham em média 40,7 anos (±17,25), casadas, autodeclaradas negras, com fundamental incompleto, do lar, sem renda, com moradia de alvenaria, própria, com até 6 cômodos, abastecidas por caixa de água comunitária, tratada. A Qualidade de Vida, apresentou escores medianos nos domínios: físico (3,18), psicológico (3,4), relações sociais (3,45) e meio ambiente (2,59). Com a realização desta pesquisa foi possível caracterizar a comunidade quilombola de Santa Luzia do Norte-AL quanto as dificuldades de acesso a saúde e geração de renda, fatos que repercutem na sua condição de saúde. Os problemas descritos neste estudo podem contribuir para que ações de saúde sejam planejadas e efetivadas com o intuito de melhorar as condições socioeconômicas e de saúde nessa comunidade, considerando-se o contexto social, político e ambiental, valorizando seus saberes e práticas tradicionais.

Abstract Quilombola communities are present in many Brazilian states living in precarious health conditions. This is due to geographic isolation, limitations to the access of the area in which they live in, and the lack of quality in the service when it is needed to be provided. Therefore, the aim of this study was to analyze the quality of life of women from a quilombola community in northeastern Brazil. It is an observational, cross-sectional and descriptive study. 160 adult women were first interviewed through a form to collect a profile and then it was applied the WHOQOL Quality of Life questionnaire – bref. It was observed that the women were on average 40.7 years old (±17.25), married, self-declared black, who did not finish elementary school, housewife, had no income, with their own masonry house, with up to 6 rooms, supplied by a box of community treated water. Quality of Life had median scores in the domains: physical (3.18), psychological (3.4), social relationships (3.45) and environment (2.59). With this research, it was possible to characterize the quilombola community of Santa Luzia do Norte-AL regarding the difficulties of access to health and income generation, issues that affect their health condition. The problems described in this study can contribute to health actions being planned and carried out in order to improve socioeconomic and health conditions in this community, considering the social, political and environmental context, valuing their traditional knowledge and practices.

https://doi.org/10.1590/1519-6984.246463

Spatio-temporal analysis of the visceral leishmaniasis in the state of Alagoas, Brazil
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Ferreira, J. R. S.

; Silva, K. M.

; Cavalcanti, M. G. S.

; Ferreira-Júnior, G. C.

; Souza, E. C.

; Magalhães, P. K. A.

; Gomes, D. C. S.

; Fonseca, S. A.

; Maranhão, T. L. G. Q.

; Rocha, M. A. N.

; Nascimento, C. M. A.

; Lima, R. F.

; Alves, L. C.

; Brayner, F. A.

; Costa, J. G. da

; Matos-Rocha, T. J.

Brazilian Journal of Biology

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2024, Volume 84 elocation e253098

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A leishmaniose visceral é uma doença tropical negligenciada. Foram avaliadas a distribuição espacial dos casos de leishmaniose visceral no estado de Alagoas. Todos os casos de LV, registrados pela secretaria de saúde, foram analisados e georreferenciados. Entre 2008 e 2017, 97,1% dos municípios apresentaram classificação esporádica de transmissão. Com a evolução temporal, a incidência de casos de leishmaniose visceral se concentrou na maioria dos municípios da microrregião de Santana do Ipanema-AL. A análise espaço-tempo, se considerada, pode promover o aprimoramento das ações de vigilância e controle da leishmaniose visceral.

Abstract Visceral leishmaniosis is a neglected tropical disease. We evaluated the spatial distribution of cases of visceral leishmaniosis in the state of Alagoas, Brazil. All cases of VL, registered by the health department, were analyzed and georeferenced. Results: Between 2008 and 2017, 97.1% of the municipalities presented sporadic classification of transmission. With temporal evolution, the incidence of cases of visceral leishmaniosis was concentrated in most municipalities in the microregion of Santana do Ipanema-AL. Space-time analysis, if considered, may promote the improvement of surveillance and control actions of visceral leishmaniosis.

https://doi.org/10.1590/1519-6984.253098

Evaluation of antibacterial and antifungal activity of antimicrobial soaps
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Santos Junior, C. J.

; Lins, F. C. C. O.

; Santos, P. O.

; Silva, V. B.

; Barros, Y. V. R.

; Araújo, M. A. S.

; Rocha, T. J. M.

; Souza, A. K. P.

Brazilian Journal of Biology

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2024, Volume 84 elocation e263364

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Os sabonetes têm um papel importante para a nossa higiene e saúde, pois eles além de ter efeito bactericida, também removem as sujeiras presentes no corpo humano. Avaliar a eficácia de sabonetes com atividade antimicrobiana de diferentes marcas comerciais vendidas no Brasil. Foram realizados ensaios da atividade antimicrobiana dos diferentes sabonetes através do método difusão em ágar frente aos microrganismos Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Acinetobacter baumannii, Proteus mirabilis e Candida albicans. Todos os sabonetes comerciais testados apresentaram formação de halo de inibição antimicrobiana frente às bactérias S. aureus e P. aeruginosa. Apenas dois sabonetes comerciais apresentaram inibição das espécies A. baumannii e C. albicans. Nenhum dos sete produtos estudados apresentou inibição das bactérias E. cloacae, P. mirabilis e E. coli. Na comparação das informações contidas nas embalagens dos produtos com os resultados obtidos durante a pesquisa realizada foram observadas divergências. Os sabonetes que apresentaram maior eficiência contra os microrganismos testados foram aqueles das apresentações 1 e 2, que se mostraram eficazes contra as espécies de bactérias S. aureus, P. aeruginosa e A. baumannii e contra a espécie de fungo C. albicans. As marcas 3, 4, 5, 6 e 7 apresentaram o mesmo resultado de sensibilidade frente as bactérias das espécies S. aureus e P. aeruginosa, com variação quantitativa apenas do halo de inibição. Houve divergência entre as informações contidas nas embalagens dos sete produtos em estudo e os resultados dos testes experimentais.

Abstract Soaps play an important role in our hygiene and health, as they not only have a bactericidal effect but also remove dirt from the human body. To evaluate the effectiveness of soaps with antimicrobial activity from different commercial brands sold in Brazil. Tests of the antimicrobial activity of different soaps were carried out through diffusion in agar against the microorganisms Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Acinetobacter baumannii, Proteus mirabilis, and Candida albicans. All commercial soaps tested transfer antimicrobial inhibition halo formation against S. aureus and P. aeruginosa bacteria. Only two commercial soaps inhibit the species A. baumannii and C. albicans. None of the seven products studied showed inhibition of E. cloacae, P. mirabilis, and E. coli bacteria. When comparing the information contained in the packaging of the products with the results obtained during a survey, divergences were observed. The soaps that provide greater efficiency against the tested microorganisms were presented in presentations 1 and 2, which become useful against the bacteria species S. aureus, P. aeruginosa, and A. baumannii and a fungus species C. albicans. Marks 3, 4, 5, 6, and 7 parallel the same sensitivity result opposite as bacteria of the species S. aureus and P. aeruginosa, with quantitative variation only of the inhibition halo. There was a divergence between the information contained in the packaging of the seven products under study and the results of the experimental tests.

https://doi.org/10.1590/1519-6984.263364

Biomonitoring of air quality in the Bodoquena microregion, Mato Grosso Do Sul: mutagenic and morphoanatomical alterations in Tradescantia pallida (rose) D.R. Hunt var. purpurea
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Salgueiro, S. A. M.

; Rocha, A. N.

; Mauad, J. R. C.

; Silva, C. A. M.

; Mussury, R. M.

Brazilian Journal of Biology

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2024, Volume 84 elocation e250100

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste trabalho foi avaliar a qualidade do ar com base no fluxo veicular das cidades localizadas em diferentes altitudes na microrregião da Bodoquena, no estado de Mato Grosso do Sul, Brasil. Para tal, foi realizado o teste de micronúcleo, por meio do bioensaio TRAD-MCN em botões jovens de Tradescantia coletadas no período entre fevereiro a novembro de 2018 em sete cidades da microrregião da Bodoquena, com diferentes intensidades de fluxo veicular. Foram avaliados os parâmetros meteorológicos, os veículos foram contados para determinar o tráfego de veículos em cada cidade e altitude. A partir da topografia Shuttle Radar (SRTM) e processamento no software Esri ArcGIS® versão 10.5.1 foi possível mapear a área com base no Modelo de Elevação. As análises morfoanatômicos foram realizadas conforme metodologia padrão. As mensurações de espessura, comprimento, largura dos tecidos e estruturas como a cutícula superior, cutícula inferior, face superior e face inferior da epiderme, hipoderme e mesófilo foram avaliadas. O maior fluxo veicular foi encontrado nas cidades de Bodoquena, Guia Lopes da Laguna, Jardim e Porto Murtinho. O horário das 17:00 às 18:00h foi o que apresentou maiores fluxo de veículos. A maior frequência de alterações mutagênicas foi encontrada na cidade de Guia Lopes, não diferindo de Bonito, Caracol e Jardim. Ao longo do biomonitoramento observou-se que as estações de verão e outono foram as que apresentaram maiores frequências de micronúcleo independente da cidade avaliada. Observou-se que a correlação entre a frequência de micronúcleos e o fluxo veicular foi baixa, apoiando a tese de que essa microrregião, embora apresente alterações mutagênicas em T. pallida, as alterações numericamente são pequenas quando comparadas a outras regiões do estado de Mato Grosso do Sul. Observou-se uma variação na espessura dos tecidos/estruturas que é variável entre as diferentes cidades e estações do ano. De forma geral os tecidos/estrutura apresentaram redução na espessura para as cidades de Nioaque e Porto Murtinho quanto aos aspectos anatômicos e morfológicos, sendo que, para o comprimento e espessura foliar não foi observado diferenças entre as cidades. Em relação as estações do ano, observou-se que no outono a espessura dos tecidos/estruturas são menores. Diante dos fatores genotóxicos e morfoanatômicos aqui avaliados, a microrregião da Bodoquena parece estar bem preservada em termos de qualidade do ar, apresentando baixa frequência de micronúcleos e redução limitada de tecidos e estruturas foliares, independentemente da estação do ano.

Abstract The objective of this study was to assess air quality in relation to vehicular traffic flow in cities located at different elevations in the Bodoquena microregion, state of Mato Grosso do Sul, Brazil. To do so, a micronucleus test was carried out using the TRAD-MCN bioassay on young Tradescantia buds collected from February to November 2018 in seven cities of the microregion with different traffic flow intensities. Meteorological parameters were evaluated, and vehicular traffic was counted to determine traffic flow in each city. With data from the Shuttle Radar Topography Mission (SRTM) and processing in Esri ArcGIS® software version 10.5.1, the regions was mapped based on an Elevation Model. Morphoanatomical analyses were performed according to standard methodology. Measurements were taken of thickness, length and width of tissues and structures, including the upper and lower cuticle, upper and lower epidermis, hypodermis and mesophyll. The greatest traffic flow was found in the cities of Bodoquena, Guia Lopes da Laguna, Jardim, and Porto Murtinho, with the period from 5:00 to 6:00 p.m. showing the highest traffic flow. The greatest frequency of mutagenic alterations was found in the city of Guia Lopes da Laguna, although the results did not differ significantly from Bonito, Caracol, and Jardim. Throughout the biomonitoring, the summer and autumn seasons showed the greatest micronuclei frequencies in all evaluated cities. Variations in the tissue/structure thickness was observed across cities and seasons, but with a decrease in thickness during autumn. In general, the tissues/structures were smaller for the cities of Nioaque and Porto Murtinho, while the anatomical and morphological characteristics of leaf length and thickness showed no differences among cities. We found limited correlation between micronuclei frequency and traffic flow, supporting the hypothesis that although mutagenic alterations are observed in T. pallida, in this microregion the changes are numerically lower when compared to other regions of the state. In light of the genotoxic and morphoanatomical factors assessed herein, the Bodoquena microregion appears to be well preserved in terms of air quality, presenting low micronuclei frequency and a limited reduction in tissues and leaf structures, regardless of the season.

https://doi.org/10.1590/1519-6984.250100 1 downloads

Optimization of Very Short Multi-Pump Discrete Raman Amplifiers based on Tellurite Fibers MultiPump Multi Pump
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Bastida, Gabriel C.

; Rocha, Helder R. de O.

; Segatto, Marcelo E. V.

; Castellani, Carlos E. S.

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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2024, Volume 23 N. 1 elocation e2024276090

Abstract: En | Text: En | PDF: En

Abstract This paper presents a numerical study and the optimization of very short discrete multi-pump Raman amplifiers using Tellurite-based optical fibers. While most studies about discrete Raman amplifiers usually focus on cases where hundreds of meters of fiber are used, here we consider a multi-pumped Raman amplifier setup with extremely short dimensions, in which the length of the gain media was varied from 2.5 to 30 meters. The optimization process was developed over an broad amplification band from 1520 to 1600 nm, considering a maximum acceptable ripple of 3 dB, and using 2, 3 and 4 pumps with individual powers always below 2.5 W. The results show that it is possible to achieve net gains as high as 10 dB for gain media around 20 meters, and close to 15 dB for 30 meters. These high gains highlight the fact that Tellurite-based optical fibers can be used for the construction of very short and compact broadband amplifiers using just a few pump lasers with modest optical powers. multipump multi Telluritebased Tellurite based multipumped pumped dimensions 25 2 5 2. 152 160 nm W 1 16

https://doi.org/10.1590/2179-10742024v23i1276090

Chemical Characterization and Evaluation of the Antitumoral Activity of Annona tomentosa R.E.Fr. on Breast (MCF-7) Three-Dimensional (3D) Tumor Spheroids REFr R E Fr R.E.Fr MCF7 MCF 7 (MCF-7 ThreeDimensional Three Dimensional 3D D (3D (MCF- (MCF
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Pinheiro, Aglaete A.

; Lee, Celina Y.-A. L.

; Santos, Anna Gabriele P. dos

; Pereira, Érica R.

; Rosário, Marcelino S. do

; Ribeiro, Diego Luis

; Serpeloni, Juliana Mara

; Rocha, Cláudia Q. da

Journal of the Brazilian Chemical Society

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2024, Volume 35 N. 8 elocation e-20240037

Abstract: En | Text: En | PDF: En

Annona tomentosa, popularly known as “araticum marolo”, is an Annonaceae with little scientific evidence regarding its phytochemistry and biological activities. Based on this, the present study carried out the chemical characterization of the methanolic extract and fractions of ethyl acetate and alkaloid of the stem bark of A. tomentosa, in addition to evaluating their influence on the viability, proliferation and migration of breast (MCF-7) cells in a three-dimensional (3D) model. The samples were analyzed by liquid chromatography tandem mass spectrometry (LC MS/MS), and the Global Natural Products Social Networking (GNPS) platform was used to construct a molecular network. 21 compounds were identified, of which 16 belong to the alkaloid class. Multicellular spheroids (MCTS) growth was reduced after 216 h of treatment, as well as their viability (ca. 50% for the extract and ca. 25% for the alkaloid fraction). The extract and the alkaloid fraction showed greater antiproliferative (at a concentration of 100 µg mL-1 after 216 h, ca. 48% for the extract and ca. 20% for the fraction) and antimigratory (at concentrations 50-500 µg mL-1 for the extract and 100-250 µg mL-1 for the fraction) effects. This study is pioneering in demonstrating the antitumor effects in a 3D culture model, also revealing its potential for the isolation of alkaloids. tomentosa araticum marolo, marolo , marolo” activities this A MCF7 MCF 7 (MCF-7 threedimensional three dimensional D (3D model LC MS/MS, MSMS MS/MS MS MS/MS) GNPS (GNPS network 2 identified 1 class MCTS (MCTS treatment ca (ca 50 25 fraction. . at 10 mL1 mL mL- 48 20 50500 500 50-50 100250 250 100-25 alkaloids (MCF- 5 4 5050 50-5 10025 100-2 (MCF 505 50- 1002 100-

https://doi.org/10.21577/0103-5053.20240037

Optimization of Hydrolysis and Identifcation of Bioactive Peptides in Brewery Yeast Residuals
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Rosa, Fernanda A. Della

; Tonin, Angelica P.

; Rocha, Beatriz S.

; Santos, Marco A. R.

; Silveira, Fabiane M.

; Cardoso-Filho, Lucio

; Ribeiro, Valquíria M. S.

; Meurer, Eduardo C.

Journal of the Brazilian Chemical Society

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2024, Volume 35 N. 3 elocation e-20230146

Abstract: En | Text: En | PDF: En

Industrial waste products are of biotechnological interest due to their abundance and can be utilized as protein sources for the production of bioactive peptides through hydrolysis. The utilization of yeast residues from the brewing industry has been demonstrated to be a viable method for obtaining protein hydrolysates. The degree of hydrolysis and soluble total protein of the hydrolysates were evaluated for optimization purposes to achieve a product with a high proportion of soluble organic nitrogen, increased levels of bioactive peptides and amino acids. Both alkaline and enzymatic hydrolysis processes were tested. The hydrolyses were carried out with the aim of large-scale implementation. The treatments were effective in liberating intracellular content and producing biologically functional hydrolysates, with degree of hydrolysis values ranging from 28 to 63% and total protein from 2.95 to 8.28% at the optimum points confrmed by statistical analysis (analysis of variance (ANOVA)). The hydrolysate produced with Alcalase® exhibited the highest peptide intensity, while the hydrolysate produced with Flavourzyme® showed the highest concentration of free amino acids. The peptides were identifed by mass spectrometry and were found to have biological activities according to the Biopep database. This study presents a quick and economically feasible approach for the implementation of a pilot project for the reuse of this waste. nitrogen acids tested largescale large scale 2 63 295 95 2.9 828 8 8.28 ANOVA. ANOVA . (ANOVA)) Alcalase intensity Flavourzyme database 6 29 9 2. 82 8.2 (ANOVA) 8. (ANOVA

https://doi.org/10.21577/0103-5053.20230146

Canonical Unified Statistical Rate Constants Using a High-Level Composite Coupled Cluster Energy Scheme for the CH4 + CH Reaction HighLevel High Level
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Leite Júnior, Jonas A.

; Rocha, Carlos M. R.

; Oliveira Filho, Antonio G. S. de

Journal of the Brazilian Chemical Society

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2024, Volume 35 N. 2 elocation e-20230107

Abstract: En | Text: En | PDF: En

We report an ab initio study on the kinetics and chemical dynamics of the CH4 + CH reaction using a coupled cluster based composite scheme that includes unrestricted coupled cluster singles and doubles with perturbative connected triples method, UCCSD(T), energies extrapolated to the complete basis set limit, in addition to core-valence, higher-order correlation, and relativistic corrections. With this protocol, the reaction enthalpies for the two main reaction channels, C2H4 + H and CH2 + CH3, are reproduced with an absolute deviation of 0.04 kcal mol–1 relative to experimental data. At the UCCSD(T)/complete basis set (CBS) level of theory, the formation of the C2H5 intermediate is barrierless, in contrast with the low submerged barrier (–1.87 kcal -mol–1 relative to the reactants) found at the UCCSD(T)/augmented correlation-consistent polarized double-zeta (aug-cc-pVDZ) level of theory. With the correlation-consistent polarized triple-zeta (cc-pVTZ) basis set, we describe structural variations for the reaction bottleneck along the reaction path, finding at least three canonical variational transition states for the temperature range from 20 to 800 K. The thermal rates were obtained via the canonical unified statistical theory (CUS), using the canonical variational transition state theory (VTST) for the inner-transition state and long-range transition state theory (LRTST) for the outer-transition state. Our calculations agree with literature measurements and show inverse Arrhenius behavior, as observed experimentally. At 298 K, the computed rate constant is 2.65 × 10–10 cm3 molecule–1 s–1 and reported experimental measurements range from 2.5 × 10–12 to 3.0 × 10–10 cm3 molecule–1 s–1. Our theoretical study represents an improvement on previous computational investigations and highlights that even relatively simple gas-phase reactions can require high levels of theory to be modeled accurately. method UCCSDT, UCCSDT UCCSD T , UCCSD(T) limit corevalence, corevalence core valence, valence core-valence higherorder higher order correlation corrections protocol channels C C2H CH3 004 0 04 0.0 mol1 mol 1 mol– data UCCSDT/complete UCCSDTcomplete /complete CBS (CBS barrierless –1.87 187 87 (–1.8 -mol– reactants UCCSDT/augmented UCCSDTaugmented /augmented augmented correlationconsistent consistent doublezeta double zeta augccpVDZ aug cc pVDZ (aug-cc-pVDZ triplezeta triple ccpVTZ pVTZ (cc-pVTZ path 2 80 K CUS, CUS (CUS) VTST (VTST innertransition inner longrange long LRTST (LRTST outertransition outer behavior experimentally 29 265 65 2.6 1010 10 10–1 cm molecule1 molecule molecule– s1 s s– 25 5 2. 1012 12 30 3 3. gasphase gas phase accurately UCCSD(T 00 0. –1.8 18 8 (–1. -mol (CUS 26 6 101 10– –1. (–1 –1 (– – (

https://doi.org/10.21577/0103-5053.20230107

DIMENSIONING OF VINYLSULFONIC SUPPORTS FROM CASHEW APPLE BAGASSE BIOMASS IN THE IMMOBILIZATION OF LIPASES
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Lima, Paula J. M.

; Silva, Jouciane de S.

; Melo, Rafael L. F.

; S. Neto, Francisco

; Fechine, Pierre B. A.

; Rocha, Maria V. P.

; Gonçalves, Luciana R. B.

; Santos, José C. S. dos

Química Nova

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2024, Volume 47 N. 5 elocation e-20230132

Abstract: En | Text: En | PDF: En

In this work, the support, cashew apple bagasse (CAB), was chemically modified with divinyl sulfone (DVS), and it was evaluated to immobilize Candida antarctica lipase A (CAL-A). The best activation conditions of CAB support were defined by an advanced experimental design using the Taguchi method, assessing five factors at four levels (concentration of DVS, ionic strength, pH, temperature, and time). The support and biocatalyst (CAB-DVS-CAL-A) were characterized by Fourier transform infrared spectroscopy (FTIR), elemental analysis, thermogravimetry (TGA), scanning microscopy (SEM), fluorescence spectroscopy (XRF) and electrophoresis. The optimal conditions to activate the support were DVS concentration of 1.4 mol L-1 (3 mL of DVS in 20 mL of reaction volume), a concentration of sodium bicarbonate buffer at 5 mmol L-1, pH 3.0 at 30 °C for 12 h. The immobilization on CAB-DVS promoted increased thermal stability at 70 °C and different pHs of CAL-A. Therefore, the selected conditions allowed for a catalyst with a catalytic activity of 6.8 U g-1 and more stable than the free enzyme (CAL-A). This demonstrates that pretreated and DVS-activated CAB is a promising support for enzyme immobilization. work CAB, , (CAB) (DVS) CALA. CALA CAL . (CAL-A) method strength temperature time. time time) CABDVSCALA (CAB-DVS-CAL-A FTIR, FTIR (FTIR) analysis TGA, TGA (TGA) SEM, SEM (SEM) XRF (XRF electrophoresis 14 1 4 1. L1 L L- 3 ( 2 volume, volume volume) L1, 1, 0 3. C h CABDVS 7 A. CAL-A Therefore 68 6 8 6. g1 g g- DVSactivated activated (CAB (DVS (CAL-A (FTIR (TGA (SEM

https://doi.org/10.21577/0100-4042.20230132

10.

The high biodiversity of benthic organisms in a coastal ecosystem revealed by an integrative approach
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Amaral, A. Cecília Z.

; Cunha, Beatriz P.

; Checon, Helio H.

; Godoy, Angélica S. de

; Silva, Camila F. da

; Corte, Guilherme N.

; Nogueira, João M. de M.

; Fukuda, Marcelo V.

; Steiner, Tatiana M.

; Kawauchi, Gisele Y.

; Turra, Alexander

; Denadai, Márcia R.

; Ferreira, Luciane

; Mendonça, Joel B. de

; Tavares, Marcos

; Leite, Fosca P. P.

; Costa, Mariana F. B.

; Siqueira, Silvana G. L.

; Vieira, Leandro M.

; Dias, Gustavo M.

; Teixeira, Joyce A.

; Rocha, Rosana M.

; Gusmão, Luciana C.

; Borges, Michela

; Alitto, Renata

; Machado, Fabrizio M.

; Passos, Flávio D.

; Cunha, Carlo M.

; Simone, Luiz R.L.

; Araujo, Ana Paula G.

; Carbayo, Fernando

; Bahia, Juliana

; Bulnes, Verónica N.

; Castello-Branco, Cristiana

; Hajdu, Eduardo

; Vilas-Boas, Ana Carolina

; Garraffoni, André R. S.

; Schockaert, Ernest

; Fonseca, Gustavo

; Domenico, Maikon Di

; Curini-Galletti, Marco

; Sørensen, Martin V.

; Hochberg, Rick

; Oliveira, Ana Julia F. C. de

; Zampieri, Bruna Del B.

; Chinelatto, Roberta M.

; Migotto, Alvaro E.

Biota Neotropica

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2024, Volume 24 N. 2 elocation e20231583

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Increasing habitat modification and species loss demand consistent efforts to describe and understand biodiversity patterns. The BIOTA/FAPESP Program was created in this context and it has been a successful initiative to promote studies on biodiversity and conservation in Brazil. The BIOTA/Araçá is an interdisciplinary project that provided a detailed evaluation of the biodiversity of Araçá Bay, a coastal seascape located on the North coast of the state of São Paulo, Southeast Brazil. The bay encompasses multiple habitats, such as beaches, mangroves, rocky shores, and a tidal flat, and provides important ecosystem services. Unfortunately, the bay is the subject of complex social-environmental conflicts that oppose economic, social, and environmental demands (i.e., the expansion of neighboring harbor activities vs. small-scale artisanal fisheries and protection of biodiversity). The present study presents a survey of the benthic species occurring in the different habitats of Araçá Bay, including data obtained during the BIOTA/Araçá project and previous assessments of the area. The benthic species play an important role in marine environments and studying the diversity of these organisms that live associated with the bottom is indispensable for comprehending the environment’s functioning. The macrofauna, meiofauna, and microorganisms associated with soft and hard bottom were listed, and additional information, such as the habitat and geographical distribution, were provided for each species. The checklist includes 826 species, almost 70% recorded during the BIOTA/Araçá project. The most speciose taxa were the annelids (225 spp.), mollusks (194 spp.), and crustaceans (177 spp.). Seven benthic species are endemic to Araçá Bay, 14 are considered threatened, and seven are economically exploited. Furthermore, the bay is the type locality of many taxa, and 11 new benthic species were described based on specimens sampled during the project. This project shows the importance of Araçá Bay as a unique biologically rich environment and highlights the need for conservation efforts in light of the current threats. patterns BIOTAFAPESP BIOTA FAPESP Brazil BIOTAAraçá Paulo beaches mangroves shores flat services Unfortunately socialenvironmental social economic i.e., ie i e (i.e. vs smallscale small scale biodiversity. . biodiversity) area s functioning macrofauna meiofauna listed information distribution 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. 1 threatened exploited Furthermore threats i.e. (i.e 8 7 22 (2 19 (1 17 i.e 2 (

Resumo O aumento da modificação dos habitats e da perda de espécies demanda esforços consistentes para descrever e compreender os padrões de biodiversidade. O programa BIOTA/FAPESP foi criado nesse contexto e é uma iniciativa de sucesso para promover estudos em biodiversidade e conservação no Brasil. O BIOTA/Araçá é um projeto interdisciplinar que promoveu uma avaliação detalhada da biodiversidade da Baía do Araçá, um ecossistema costeiro localizado ao Norte do estado de São Paulo, Sudeste do Brasil. A baía engloba múltiplos habitats, tais como praias, manguezais, costões rochosos, e uma planície de maré, e também fornece importantes serviços ecossistêmicos. Infelizmente, a baía está sujeita à conflitos sócio-ambientais complexos que contrastam demandas econômicas, sociais e ambientais (i.e. a expansão das atividades do porto vizinho vs. a pesca artesanal de pequena escala e a proteção da biodiversidade). O presente estudo apresenta um levantamento das espécies bentônicas que ocorrem nos diferentes habitats da Baía do Araçá, incluindo dados obtidos durante o projeto BIOTA/Araçá e de investigações realizadas anteriormente na área. As espécies bentônicas desempenham um papel importante no ambiente marinho, e estudar a diversidade desses organismos que vivem associados ao fundo é indispensável para compreender o funcionamento do meio ambiente. A macrofauna, meiofauna, e microorganismos associados aos fundos consolidado e inconsolidado foram listados, e informações adicionais foram fornecidas para cada espécie, tais como a distribuição geográfica e nos habitats. O checklist inclui 826 espécies, quase 70% registradas durante o projeto BIOTA/Araçá. Os taxa mais especiosos foram os anelídeos (225 spp.), moluscos (194 spp.), e crustáceos (177 spp.). Entre as espécies bentônicas listadas, sete são endêmicas da Baía do Araçá, 14 são consideradas ameaçadas de extinção, e sete são exploradas economicamente. A baía é a localidade tipo de vários taxa, e 11 novas espécies bentônicas foram descritas com base em espécimes amostrados durante o projeto. Este projeto mostra a importância da Baía do Araçá como um ambiente de riqueza biológica única e demonstra a necessidade de esforços para a sua conservação considerando as atuais ameaças. BIOTAFAPESP BIOTA FAPESP Brasil BIOTAAraçá Paulo praias manguezais rochosos maré ecossistêmicos Infelizmente sócioambientais sócio econômicas i.e. ie i (i.e vs . biodiversidade) área marinho macrofauna meiofauna listados espécie 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. listadas 1 extinção economicamente ameaças i.e 8 7 22 (2 19 (1 17 2 (

https://doi.org/10.1590/1676-0611-bn-2023-1583

11.

Forage performance in silage production under water deficit conditions
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Pires, Fábio R.

; Rocha Júnior, Paulo R. da

; Cavalcanti, André C.

; Nascimento, Adriel L.

; Nardotto Júnior, André O.

; Almeida, Marcelo S. de

; Viana, Douglas G.

; Bonomo, Robson

Revista Caatinga

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2024, Volume 37 elocation e12345

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT The positive performance of different cultivars in relation to a water deficit is directly correlated with an increase in biomass production, with repercussions for ensuring system sustainability. The objective of this study was to evaluate the production and nutritional value of four millet genotypes in comparison with that of corn and sorghum under two water supply conditions (simulated water deficit and normal conditions). This experiment was conducted using a completely randomized design (CRD), with six cultivars, two irrigation levels, and four replicates, totaling 48 experimental units. At the end of 12 weeks after sowing, biometric and bromatological evaluations were conducted. Based on these results, pearl millet ADR300, ADR500, BRS1501, and ADRf6010 showed promise and are interesting alternatives to be explored by silage producers in more critical water deficit periods. Among millets, ADRf6010 performed similarly to corn and sorghum, which are commonly used in Brazil as forage, indicating that it is the best alternative for silage formation. sustainability simulated conditions. . conditions) CRD, CRD , (CRD) levels replicates 4 units 1 sowing results ADR300 ADR ADR500 BRS1501 BRS ADRf ADRf601 periods millets forage formation (CRD ADR30 ADR50 BRS150 ADRf60 ADR3 ADR5 BRS15 ADRf6 BRS1

RESUMO O desempenho positivo de diferentes cultivares ao déficit hídrico está diretamente correlacionado com o aumento da produção de biomassa, com reflexos na garantia da sustentabilidade do sistema. Objetivou-se com esse estudo avaliar a produção e valor nutritivo de quatro cultivares de milheto em comparação com o milho e sorgo, sob duas condições de fornecimento de água (simulando uma condição de déficit hídrico e uma condição normal). O delineamento experimental foi inteiramente ao acaso (DIC), com 6 cultivares, 2 níveis de irrigação e 4 repetições, totalizando 48 unidades experimentais. Ao final de 12 semanas após a semeadura, foram realizadas as avaliações biométricas e bromatológicas. Com base nos resultados, os milhetos ADR300, ADR500, BRS1501 e ADRf6010 se apresentaram promissores e são alternativas interessantes a serem exploradas por produtores para silagem em períodos mais críticos ao déficit hídrico. Dentre os milhetos, o ADRf6010 apresentou desempenho semelhante quando comparado com o milho e sorgo, comumente utilizados no Brasil como forragem, indicando ser a melhor alternativa para a formação de silagem. biomassa sistema Objetivouse Objetivou sorgo simulando normal. normal . normal) DIC, DIC , (DIC) repetições experimentais 1 semeadura bromatológicas resultados ADR300 ADR ADR500 BRS BRS150 ADRf ADRf601 forragem (DIC ADR30 ADR50 BRS15 ADRf60 ADR3 ADR5 BRS1 ADRf6

https://doi.org/10.1590/1983-21252024v3712345rc

12.

Zinc oxide nanoparticles and bioinoculants on the postharvest quality of eggplant subjected to water deficit
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Martins, Bruna L. R.

; Araújo, Railene H. C. R.

; Rocha, Josinaldo L. A.

; Silva, Toshik I. da

; Alves, Kalinny de A.

; Almeida, Evanilson S. de

; Ferreira, Kaiki N.

Revista Brasileira de Engenharia Agrícola e Ambiental

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2024, Volume 28 N. 7 elocation e279019

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A berinjela (Solanum melongena) é amplamente cultivada. Apresenta tolerância moderada ao défice hídrico, mas sofre perdas de rendimento nas regiões áridas e semiáridas onde é cultivada. O objetivo deste estudo foi investigar a influência das nanopartículas de óxido de zinco (NPZnO), em associação com bactérias promotoras de crescimento vegetal (BPCV), na qualidade pós-colheita de berinjela submetida a déficit hídrico. Foram estudadas duas percentagens de irrigação referentes à evapotranspiração potencial -ETo (50 e 100% de ETo) e cinco combinações envolvendo NPZnO ou BPCV. Foram avaliados peso e número de frutos comerciais e não comerciais, diâmetro, comprimento, cor da casca, firmeza, acidez titulável, sólidos solúveis, SS/TA, vitamina C e açúcares solúveis totais. Houve forte correlação positiva entre peso de frutos comerciais, SS/TA, açúcares solúveis totais, acidez titulável, luminosidade e vitamina C nos tratamentos contendo ZnSO₄, NPZnO e BPCV. O déficit hídrico e as nanopartículas contendo zinco, associadas ou não a bactérias promotoras do crescimento das plantas, não influenciaram o peso e o tamanho médio dos frutos e a qualidade pós-colheita da cultura da berinjela. O déficit hídrico reduziu a cromaticidade e a luminosidade da cor da casca e o teor de vitamina C da berinjela. Solanum melongena cultivada NPZnO, , (NPZnO) BPCV, BPCV (BPCV) póscolheita pós colheita ETo 50 (5 100 diâmetro comprimento firmeza titulável SSTA SS TA SS/TA totais ZnSO₄ ZnSO plantas (NPZnO (BPCV 5 ( 10 1

ABSTRACT Eggplant (Solanum melongena) is widely cultivated. It shows moderate tolerance to water deficit, but suffers yield losses in the arid and semi-arid regions where it is grown. The aim of this study was to investigate the influence of zinc oxide nanoparticles (NPZnO), in association with plant growth-promoting bacteria (PGPB), on the post-harvest quality of eggplant subjected to water deficit. Two irrigation percentages relative to potential evapotranspiration-ETo (50 and 100% ETo) and five combinations involving NPZnO or PGPB were studied. Number of commercial fruits per plant and weight of commercial fruits per plant, diameter, length, skin color, firmness, titratable acidity, soluble solids, SS/TA, vitamin C and total soluble sugars were evaluated. There was strong positive correlation between weight of commercial fruits per plant, SS/TA, total soluble sugars, titratable acidity, lightness and vitamin C in the treatments containing ZnSO₄, NPZnO and PGPB. Water deficit and nanoparticles containing zinc, associated or not with bacteria that promote plant growth, did not influence the weight and average size of the fruits and the post-harvest quality of the eggplant crop. Water deficit reduced the chromaticity and lightness of the skin color and the vitamin C content of eggplant. Solanum melongena cultivated semiarid semi grown NPZnO, , (NPZnO) growthpromoting growth promoting PGPB, (PGPB) postharvest post harvest evapotranspirationETo evapotranspiration ETo 50 (5 100 studied diameter length firmness acidity solids SSTA SS TA SS/TA evaluated ZnSO₄ ZnSO crop (NPZnO (PGPB 5 ( 10 1

https://doi.org/10.1590/1807-1929/agriambi.v28n7e279019

13.

Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer Longterm Long term VEIL (VEIL
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Tobias-Machado, Marcos

; Ornellas, Antonio A.

; Hidaka, Alexandre K.

; Medina, Luis G.

; Mattos, Pablo A. L.

; Besio, Ruben S.

; Abreu, Diego

; Castro, Pedro R.

; Nishimoto, Ricardo H.

; Astigueta, Juan

; Dourado, Aurus

; Machado, Roberto D.

; Magnabosco, Wesley J.

; Corona-Montes, Victor

; Villoldo, Gustavo M.

; Zampolli, Hamilton C.

; Taha, Anis

; Auad, Pericles R.

; Faria, Eliney F.

; Arantes, Paulo B. O.

; Tavares, Alessandro

; Nascimento, Francisco S. M. S.

; Brazão Jr., Eder S.

; Rocha, Maurício M.

; Costa, Walter H.

; Panico, Vinicius

; Reis, Leonardo O.

; Almeida-Carrera, Roberto J.

; Silva, Rafael C.

; Zequi, Stênio C.

; Calixto, José R. R.

; Sotelo, Rene

International braz j urol

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oct 2023, Volume 49 N. 5 Pages 580 - 589

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer. Objective VEIL. . (VEIL) Methods 2 CoalitionLatin Coalition Latin PeCLA PeC LA (PeC-LA technique intermediate/highrisk intermediatehighrisk intermediate/high risk intermediate high nonfixed non fixed diameter range Results 200 2020 5 4568 45 68 (45-68 old 9 60120. 60120 60 120 (60-120) 1 616. 616 6 16 (6-16) 157 15 7 15.7% 19 1.9 86 8 8. 48 4.8 respectively 267 26 26.7 nonpalpable 28 2.8 10y y 742 74 74.2 848 84 84.8% pN0 pN pN1 100 100% 824 82 82.4% 727 72 72.7 91 9.1% Conclusion morbidity noninvasive invasive biopsy nonbulky bulky (VEIL highrisk intermediatehigh 20 202 456 (45-6 6012 12 (60-120 61 (6-16 15.7 1. 4. 26. 2. 74. 84.8 82.4 72. 9.1 (45- 601 (60-12 (6-1 15. 84. 82. 9. (45 (60-1 (6- (4 (60- (6 ( (60

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

14.

Proximate composition, minerals, tannins, phytates and cooking quality of commercial cowpea cultivars composition minerals tannins
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Oliveira, Adolfo M. C. de

; Jean, Abdias

; Damasceno-Silva, Kaesel J.

; Moreira-Araújo, Regilda S. dos R.

; Franco, Luis J. D.

; Rocha, Maurisrael de M.

Revista Caatinga

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sep 2023, Volume 36 N. 3 Pages 702 - 710

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Cowpea is a socioeconomically important legume in the Northeast region of Brazil, and the most grown and consumed type of bean, representing the main source of low-cost vegetable protein for rural and urban populations in this region. The objective of this work was to characterize the proximate composition, minerals, tannins, phytic acid, and cooking quality of whole grains of cowpea from the commercial cultivars BRS Aracê, BRS Inhuma, and BRS Xiquexique. Grain samples of the cultivars were ground in a zirconium ball mill and the flour was used for analysis. The proximate composition was carried out according to the AOAC methodology. The minerals were determined by nitro-perchloric digestion and reading in a flame atomic absorption spectrophotometer. The anti-nutritional factors tannins and phytic acid were determined by extraction and reading in a spectrophotometer. Cooking quality was assessed using an electric pressure cooker and then a Mattson cooker. A completely randomized experimental design was used, with three treatments (cultivars) and three replications. The data were subjected to analysis of variance and the means were compared by the Tukey's test (p<0.05). The cowpea cultivars showed significant differences (p<0.05) for most characteristics evaluated and were similar in terms of ash, lipids, total dietary fiber, and most macrominerals. The results showed that grains of the cultivars BRS Aracê and BRS Xiquexique are excellent sources of dietary fiber, proteins, and minerals and present high cooking quality, while grains of the cultivar BRS Inhuma stood out regarding carbohydrates, soluble dietary fibers, total energetic value, and low levels of factors, anti-nutritional tannins and phytic acid. Brazil bean lowcost cost methodology nitroperchloric nitro perchloric spectrophotometer antinutritional anti nutritional (cultivars replications Tukeys Tukey s p<0.05. p005 p p<0.05 . 0 05 (p<0.05 ash lipids fiber macrominerals proteins carbohydrates fibers value p00 p<0.0 (p<0.0 p0 p<0. (p<0. p<0 (p<0 p< (p< (p

RESUMO O feijão-caupi é uma leguminosa de grande importância socioeconômica na região Nordeste do Brasil, sendo o tipo de feijão mais cultivado e consumido, representando a principal fonte de proteína vegetal de baixo custo das populações rural e urbana dessa região. O objetivo deste trabalho foi caracterizar a composição centesimal, minerais, taninos, ácido fítico e a qualidade de cozimento dos grãos integrais das cultivares de feijão-caupi BRS Aracê, BRS Inhuma e BRS Xiquexique. A composição centesimal foi determinada de acordo com a metodologia AOAC. Os minerais foram determinados por digestão nitro-perclórica e leitura em espectrofotômetro de absorção atômica de chama. Os fatores antinutricionais taninos e ácido fítico foram determinados por extração e leitura em espectrofotômetro. A qualidade do cozimento foi avaliada usando uma panela de pressão elétrica e, em seguida, o cozedor de Matsson. Foi utilizado o delineamento inteiramente casualizado (DIC), com três tratamentos (cultivares) e três repetições. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey (p<0,05). As cultivares apresentaram diferenças significativas (p<0,05) para a maioria das características avaliadas e similares para os teores de cinzas, lipídeos, fibra alimentar total e a maioria dos macrominerais. As cultivares BRS Aracê e BRS Xiquexique representam excelentes fontes de fibras dietéticas, proteínas e minerais, além de alta qualidade de cozimento, enquanto a cultivar BRS Inhuma destacou-se em carboidratos, fibras alimentares solúveis, valor energético total e baixos teores dos fatores antinutricionais analisados. feijãocaupi caupi Brasil consumido AOAC nitroperclórica nitro perclórica chama seguida Matsson DIC, DIC , (DIC) (cultivares repetições p<0,05. p005 p p<0,05 . 0 05 (p<0,05 cinzas lipídeos macrominerais dietéticas destacouse destacou se carboidratos solúveis analisados (DIC p00 p<0,0 (p<0,0 p0 p<0, (p<0, p<0 (p<0 p< (p< (p

https://doi.org/10.1590/1983-21252023v36n322rc

15.

COVID-19 Infection Changes the Functions and Morphology of Erythrocytes: A Multidisciplinary Study COVID19 COVID 19 COVID-1 Erythrocytes COVID1 1 COVID-
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Sales, Marcos V. S.

; Tanabe, Eloiza L. L.

; Maciel, Thamilla M. S.

; Tavares, Maria C.

; Leal, Juliana G. C.

; Pinto, Larissa S.

; Pires, Keyla S. N.

; Coelho, Jorge A. P. M.

; Silva, Elaine C. O.

; Souza, Samuel T.

; Fonseca, Eduardo J. S.

; Fragoso, Thiago S.

; Aquino, Thiago M.

; Borbely, Alexandre U.

; Rocha, Ueslen

; Santos, Josué C. C.

; Leite, Ana Catarina R.

Journal of the Brazilian Chemical Society

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aug 2023, Volume 34 N. 8 Pages 1185 - 1196

Abstract: En | Text: En | PDF: En

Hematological problems are associated with Coronavirus disease 2019 (COVID-19). Respiratory impairment is the higher point studied, although without experimental studies related to the oxygen transport performed by erythrocytes. Therefore, we decided to investigate if erythrocytes from COVID-19 patients have their functionality changed. The case-control study included hospitalized patients with a positive real-time polymerase chain reaction (RT-PCR) result admitted to University Hospital. Volunteers (negative RT-PCR results) were recruited as a control group. Thus, we assessed different erythrocytes parameters, oxidative stress markers, and biophysical studies using whole blood and isolated hemoglobin. We found a decrease of 51% in oxygen uptake and reduced antioxidant enzyme activity in COVID-19 patients compared to controls. Raman spectrometry showed structural changes in the hemoglobin and lipids of the erythrocytes from COVID-19 patients; thus, these results were consolidated with an increase in Young’s modulus in erythrocytes followed by morphology changes. Besides, isolated hemoglobin from COVID-19 patients has a distinct interaction profile using a ligand model compared to the control. COVID-19 leads to structural, functional, and morphological damage to human erythrocytes. Our data showed structural and molecular changes and induction of oxidative stress in erythrocytes by COVID-19, a new perspective on the contribution of erythrocytes to a respiratory commitment in COVID-19. 201 COVID19. COVID19 COVID 19 . (COVID-19) studied Therefore COVID-1 changed casecontrol case realtime real time RTPCR RT PCR (RT-PCR Hospital negative group Thus parameters markers 51 controls thus Youngs Young s Besides functional COVID19, 19, 19. 20 COVID1 1 (COVID-19 COVID- 5 2 (COVID-1 (COVID- (COVID

https://doi.org/10.21577/0103-5053.20230031

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