To evaluate the risks of hair dye exposure, we investigated cellular and molecular effects of Arianor Ebony dye, which is a mixture of azo and anthraquinone dyes, used in the composition of the black color. Cytotoxicity, genotoxicity, and gene expression of relevant molecules of apoptotic and oxidative stress mechanisms were investigated in HepG2 cells exposed to Arianor Ebony. Results showed that the dye did not induce cytotoxicity to exposed cells at a concentration up to 50 µg/mL compared to the negative control. However, genotoxic assays indicated that the dye was able to damage the genetic material at a concentration of 25 µg/mL, with induction factor values of exposed cells two- to five-fold higher than those recorded for the negative control. Moreover, the lowest observed effect concentration was 12.5 µg/mL. For gene expression, relevant changes were observed in cytochrome c and caspase 9, which decreased in cells incubated with the dye in a dose-dependent manner when compared with the negative control. In parallel, the expression of genes for antioxidant enzymes was increased in exposed cells, suggesting the presence of metabolic routes that protect cells against the toxic effect of the dye, avoiding exacerbated cellular death. Results suggested that the dye disrupted cellular homeostasis through mitochondrial dysfunction, which may be hazardous to human health. Thus, further investigations are necessary to deeply understand the mechanisms of action of the dye, considering its toxic potential found in our ex vivo assays.

Resumen Se sintetizaron una serie de catalizadores de paladio (0.5, 1.5 y 3.0% en peso), mediante impregnación a volumen de poro en un tamiz molecular mesoporoso a base de sílice (SBA-15). Todos los materiales se caracterizaron por Fisisorción de N2, DRX, MEB y HR-TEM. La actividad catalítica se evaluó en la oxidación catalítica vía húmeda (OCVH) del fenol a 160°C, 10 bar, 200 mg de catalizador y 200 mL de fenol de una solución de 500 ppm. Los resultados de la caracterización muestran una gran influencia del contenido metálico de Pd; el área específica disminuye al introducir un mayor porcentaje de paladio. Los materiales presentaron textura, estructura y morfología acorde a lo reportado en la literatura. Se encontró que el tamaño de las especies de óxido de paladio soportadas, formadas durante la etapa de calcinación fue menor en el catalizador con menor contenido metálico (Pd0.5/SBA-15) y este fue el más activo en la degradación de fenol y degradó 64 % de fenol, siendo el más activo de los catalizadores.

Abstract A series of palladium catalysts (0.5, 1.5 and 3.0 wt%) were synthesized by impregnation at pore volume on a silica-based mesoporous molecular sieve (SBA-15). Materials were characterized by N2 physisorption, X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (HR-TEM). The catalytic activity was evaluated in catalytic wet air oxidation (CWAO) of phenol at 160oC, 10 bar, 200 mg of catalyst and 200 mL of phenol from a 500 ppm solution. The results of the characterization show a great influence of the metallic content of Pd; the specific area decreases when introducing a higher percentage of palladium. The materials presented texture, structure and morphology according to what has been observed in literature. It was found that the size of the supported palladium oxide species formed during the calcination stage was smaller in the catalyst with lower metal content (Pd0.5/SBA-15) and this was the most active in the degradation of phenol and degraded 64% of phenol, being the most active of the catalysts.

Fetuses exposed to alcohol and/or tobacco are at risk for perinatal adversities. However, little is currently known about the association of the separate or concomitant use of alcohol and tobacco with infant motor and cognitive development. Thus, the objective of the present study was to investigate the association between maternal consumption of alcohol and/or tobacco during pregnancy and the motor and cognitive development of children starting from the second year of life. The study included 1006 children of a cohort started during the prenatal period (22-25 weeks of pregnancy), evaluated at birth and reevaluated during the second year of life in 2011/2013. The children were divided into four groups according to the alcohol and/or tobacco consumption reported by their mothers at childbirth: no consumption (NC), separate alcohol consumption (AC), separate tobacco consumption (TC), and concomitant use of both (ACTC). The Bayley Scale of Infant and Toddler Development Third Edition screening tool was used for the assessment of motor and cognitive development. Adjusted Poisson regression models were used to determine the association between groups and delayed development. The results indicated that only the ACTC group showed a higher risk of motor delay, specifically regarding fine motor skills, compared to the NC group (RR=2.81; 95%CI: 1.65; 4.77). Separate alcohol or tobacco consumption was not associated with delayed gross motor or cognitive development. However, the concomitant use of the two substances increased the risk of delayed acquisition of fine motor skills.

This study aimed to estimate and compare racial inequality in low birth weight (LBW), preterm birth (PTB), and intrauterine growth restriction (IUGR) in two Brazilian birth cohorts. This was a cross-sectional study nested within two birth cohorts in Ribeirão Preto (RP) and São Luís (SL), whose mothers were interviewed from January to December 2010. In all, 7430 (RP) and 4995 (SL) mothers were interviewed. The maternal skin color was the exposure variable. Associations were adjusted for socioeconomic and biological covariates: maternal education, per capita family income, family economic classification, household head occupation, maternal age, parity, marital status, prenatal care, type of delivery, maternal pre-pregnancy BMI, hypertension, hypertension during pregnancy, and smoking during pregnancy collected from questionnaires applied at birth. Statistical analysis was done with the chi-squared test and logistic regression. In RP, newborns from mothers with black skin color had a higher risk of LBW and IUGR, even after adjusting for socioeconomic and biological variables (P<0.001). In SL, skin color was not a risk factor for LBW (P=0.859), PTB (P=0.220), and IUGR (P=0.062), before or after adjustment for socioeconomic and biological variables. The detection of racial inequality in these perinatal outcomes only in the RP cohort after adjustment for socioeconomic and biological factors may be reflecting the existence of racial discrimination in the RP society. In contrast, the greater miscegenation present in São Luís may be reflecting less racial discrimination of black and brown women in this city.

RESUMO A cutia (Dasyprocta agouti) e a capivara (Hydrochoerus hydrochaeris) são roedores histricomorfos da fauna brasileira. A anatomia de animais silvestres é essencial para a prática clínico-cirúrgica e programas conservacionistas. Este trabalho objetivou avaliar a morfologia quantitativa do coração da cutia e da capivara. Para isso, foram utilizados três corações de cutias e três de capivaras, provenientes do acervo do Setor de Anatomia dos Animais Domésticos e Silvestres da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. Os corações foram pesados e as mensurações foram realizadas com paquímetro. O volume do ventrículo esquerdo foi calculado pelo método de Cavalieri. O peso médio dos corações foi de 16,76g (cutias) e de 104,2g (capivaras). Os valores médios para as dimensões eixo, largura e base foram, para a cutia, de 4,3cm, 3,3cm e 3,3cm e para a capivara, de 7,6cm, 6,0cm e 6,1cm, respectivamente. O volume médio do ventrículo esquerdo da cutia e da capivara foi respectivamente de 5,03cm3 e de 54,55cm3. Os resultados quantitativos dos corações foram compatíveis com o peso corporal médio dos roedores. Assim, esses dados numéricos podem ser aplicados à cardiologia veterinária.

ABSTRACT The cutia (Dasyprocta agouti) and capybara (Hydrochoerus hydrochaeris ) are hystricomorphic rodents of the Brazilian fauna. The wild animal anatomy is essential to the clinic and surgery practices and conservation programs. This work aimed to evaluate the heart quantitative morphology of the cutia and the capybara. For this, three hearts of cutia and three of capybaras were used from the collection of the Anatomy Sector of Domestic and Wild Animals of the Faculty of Veterinary Medicine and Animal Science of the University of São Paulo. The hearts were weighted and measured with a pachymeter. The left ventricle volume was estimated through the Cavalieri method. The hearts’ mean weight was 16.76g (cutias) and 104.2g (capybaras). The mean values for the axis, width and base dimensions were of the cutia 4.3cm, 3.3cm and 3.3cm and for the capybara were 7.6cm, 6.0cm and 6.1cm, respectively. The mean volume of the left ventricle of the cutia and the capybara was 5.03cm3 and 54.55cm3, respectively. The quantitative results of the hearts were compatible with the average body weight of the rodents. Thus, these numerical data can be applied to veterinary cardiology.

RESUMO A Peritonite Infecciosa Felina (PIF) é uma doença progressiva e fatal de felinos domésticos e selvagens, causada pelo vírus da Peritonite Infecciosa Felina (FIPV). A doença é caracterizada por uma reação imunomediada contra o vírus em vários órgãos. Este trabalho descreveu um relato de caso de epicardite fibrinosa causada pelo FPIV. Um gato macho, com três anos de idade, veio a óbito e foi recebido para necropsia. Macroscopicamente, foi observado exsudato fibrinoso esbranquiçado, friável, multifocal, medindo até 2 centímetros de espessura, no epicárdio, principalmente no ápice cardíaco. Microscopicamente, foi observado no epicárdio um infiltrado inflamatório misto, multifocal, composto por macrófagos, plasmócitos e linfócitos, associado a deposição de fibrina. A imuno-histoquímica foi positiva para o FIPV nas áreas de inflamação no epicárdio. Os autores descrevem o segundo relato de caso na literatura científica de epicardite causada pelo FIPV em um gato. Portanto, a epicardite deve ser considerada como diagnóstico diferencial em doenças cardíacas em Medicina Felina.

ABSTRACT Feline Infectious Peritonitis (FIP) is a progressive and fatal disease in domestic and wild cats, caused by Feline Infectious Peritonitis Virus (FIPV). The disease is characterized by an immunomediated reaction against the virus in various organs. This work described a case report of fibrinous epicarditis caused by FIPV. A male cat, three years old, died and was received to be necropsied. Grossly, soft, multifocal to coalescing, whitish fibrinous exudate, measuring up the 2 centimeters of thickness, was observed in the epicardium, mostly at the apex of the heart. Microscopically, severe, multifocal to coalescing inflammatory infiltrate was observed in the epicardium, composed mainly by macrophages, plasmocytes and lymphocytes, associated with fibrin deposition. Immunohistochemistry was performed for FIPV and was positive in the areas of inflammation in the epicardium. To the author´s knowledge, this is the second report of epicarditis due to FIPV in a cat. Therefore, epicarditis should be considered a differential diagnosis of cardiac diseases in Feline Medicine.

Abstract: The genus Capsicum is an economically important plant around the world. In Mexico, chili cropping is a profitable agricultural business due to its demand in the national and international market. A species of interest is the piquín chili pepper, Capsicum annuum var. glabriusculum (Dunal) Heiser & Pickersgill, whose exploitation is limited only to wild populations. There have been many unsuccessful attempts to cultivate it intensively because of its low germination percentage, since the seed shows non-deep physiological dormancy, a frequent feature in undomesticated wild species. In this study, the effect of temperature and times of storage of piquín chili pepper seed on the concentration of tryptophan (auxin precursor amino acid), cytokinins (kinetin) and gibberellins (GA3) and their germinative capacity were analyzed. For this, the germinative capacity of pepper seeds stored at two temperatures (4 and 24 °C) and five times (0, 3, 6, 9 and 12 months) was evaluated. Also, quantification of the phytohormones auxins, cytokinins and gibberellins was performed using high performance liquid chromatography (HPLC). The results show that the content of the three phytohormones had increased through time according to the time of storage. The highest germination percentage was at 9 months of storage and this was the highest content of the three phytohormones; however, at 12 months of storage, germination started diminishing as well as the phytohormone content, indicating that the seed quality and viability was starting to decrease.

Resumen: El género Capsicum es una hortaliza de importancia económica a nivel mundial. En México es una importante actividad económica en el sector agrícola, por ello la importancia del cultivo de variedades que permitan abrir nuevas áreas de oportunidad comercial tanto a nivel nacional como internacional. Una de estas variedades es el chile piquín Capsicum annuum var. glabriusculum (Dunal) Heiser & Pickersgill, cuyo aprovechamiento se reduce únicamente a poblaciones silvestres, ya que se han hecho varios intentos de cultivarla de manera intensiva teniendo como resultado bajos porcentajes de germinación, debido a que la semilla presenta latencia fisiológica no profunda, rasgo característico de especies que aún no han sido domesticadas. En este estudio se propuso conocer el efecto de la temperatura y el tiempo de almacenamiento, sobre el contenido de triptófano (aminoácido precursor de auxinas), citocininas (kinetina) y giberelinas (GA3) presentes en la semilla y su relación en el proceso de germinación. Para evaluar la capacidad de germinación de las semillas de chile piquín, estas se almacenaron a dos temperaturas (4 °C y 24 °C) y cinco tiempos de almacenamiento (0, 3, 6, 9 y 12 meses). Además, se cuantificó el contenido de fitohormonas, auxinas, citicininas y giberelinas utilizándose la técnica de Cromatografía Líquida de Alta Resolución (HPLC). Los resultados muestran que el contenido de las tres fitohormonas se incrementó con respecto al tiempo de almacenamiento. El más alto porcentaje de germinación y concentración de fitohormonas se presentó a los 9 meses de almacenamiento; mientras que, a los 12 meses, la germinación empieza a disminuir, así como el contenido de fitohormonas, indicando que la calidad y viabilidad de la semilla ha empezado a decrecer.

Rheumatoid arthritis (RA), psoriatic arthritis (PsA), and ankylosing spondylitis (AS) are inflammatory diseases with different bone remodeling patterns. Fibroblast-like synoviocytes (FLS) are cells involved in the transition from an acute and reparable phase to a chronic and persistent stage in these diseases. The distinction of joint phenotypes involves inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-17, and IL-22 directly or through key signaling pathways such as Wnt. To evaluate the role of FLS as the source of Wnt antagonists (sFRP3/FRZB and Dkk1) in the synovia, levels of TNF- α, IL-17, IL-22, Dkk1, and sFRP3 were measured by ELISA directly in the synovial fluid of patients with RA, PsA, or AS. Dkk1 and sFRP3 were also measured in the FLS culture supernatants after different inflammatory stimulus. sFRP3 and Dkk1 are constitutively expressed by FLS. IL-22 and sFRP3 were positively correlated (r=0.76; P<0.01) in synovial fluid. The stimulation of FLS with IL-22, but not TNF-alpha and IL-17, increased the production of sFRP3. No stimulus altered the basal expression of Dkk1. These results showed, for the first time, the ability of IL-22 to increase the expression of sFRP3/FRZB by human FLS in both in vitro and ex vivo models. This finding linked IL-22 to local inhibition of Wnt signaling and possibly to blockade of osteogenesis. Furthermore, FLS presented as a source of this inhibitor in synovial fluid, assigning to this cell a bone injury mechanism.

Abstract Kisakiellus aweti Sousa and Elmoor-Loureiro, 2018 (Cladocera, Chydoridae) was described from first-order streams in Upper Xingu River basin. Here, we report a new occurrence of this species from Araguari river, Amapá, Brasil, extending its distribution range for more than 1,500 km in Amazon Hydrogeographic Region.

ABSTRACT The aim of this study was to evaluate a new source of injectable organic zinc (zinc edetate) on the energy and oxidative profile in sheep during the immediate postpartum period. Twenty-six Texel breed animals were previously identified and divided into two experimental groups: the treated group (TG; n= 13) that comprised the animals that received a subcutaneous (SC) injection of 100 mg of zinc edetate (2 mL) fifteen days before the parturition expected date and the control group (CG; n=13) that comprised the animals that received 2mL of physiological solution at the same date of TG. Blood samples were collected on the parturition day for the assessment of serum fructosamine, cholesterol and triglycerides, insulin-like growth factor type 1 (IGF-1), the oxidative stress index (OSi) and blood zinc concentration. In addition to these parameters, the measurement of zinc was made in food given to the animals. There was no difference in metabolic parameters and OSi between the experimental groups (P>0.05), as well as in blood zinc concentrations (P>0.05). The parenteral zinc edentate does not change the energy and oxidative profile of sheep in immediate postpartum.

RESUMO O presente trabalho foi realizado com o objetivo de avaliar a sensibilidade dos valores genéticos dos pesos corporais e as características de carcaças de codornas europeias às mudanças do gradiente ambiental (níveis da relação treonina com a lisina das dietas), do nascimento aos 21 dias de idade, por meio de modelos de regressão aleatória com diferentes classes de variância residual. Os dados utilizados neste estudo são provenientes de 915 codornas de corte da linhagem LF1 e 839 da linhagem LF2, pertencentes ao Programa de Melhoramento Genético da Universidade Federal dos Vales do Jequitinhonha e Mucuri. Foram avaliados os pesos corporais e os rendimentos da carcaça das aves. As sensibilidades dos valores genéticos às mudanças nos níveis da relação treonina:lisina (interação genótipo x ambiente) foram obtidas por modelos de regressão aleatória (utilizando normas de reação) por meio do programa Wombat, que utiliza o princípio da máxima verossimilhança restrita (REML). O modelo de regressão aleatória que considerou duas classes de variância residual foi o mais indicado para a maioria das análises realizadas. Verificaram-se alterações na classificação dos valores genéticos para as duas linhagens de codornas de corte estudadas. Esse comportamento indica sensibilidade de valores genéticos aditivos às mudanças nutricionais, o que caracteriza a existência de interação genótipo x ambiente. A predição dos valores genéticos deve ser feita com o mesmo nível da relação treonina:lisina da dieta com a qual as codornas serão alimentadas no sistema de produção.

ABSTRACT This research was carried out to evaluate the sensitivity of breeding values of body weight and carcass traits in two lines of European quails (LF1 and LF2) to changes in the environment gradient (levels of threonine: lysine ratio of diets) from hatch to 21 days of age in two lines LF1 and LF2 using Random Regression Models with different classes of residual variance. Records are from 915 quails of line LF1 and 839 of line LF2 belonging to the Breeding Improvement Program of Universidade Federal dos Vales do Jequitinhonha e Mucuri. Live body weight and weights and yields of carcass, breast, and thigh and drumstick were measured. The sensitivities of breeding values to changes in threonine: lysine ratios (genotype x environment interaction) of diets were obtained by random regression models (reaction model) using the WOMBAT program using the Restricted Maximum Likelihood principle. Model considering two classes of residual variance showed the best goodness of fit. The Reaction Norms analyses indicated changes in the ranking of breeding values for both lines suggesting quails selected in one level of threonine: lysine ratio will not express all their genetic potential if fed different threonine: lysine ratio diets. This behavior indicates sensitivity of breeding values to changes in the nutrition characterizing the genotype by environment interaction. The prediction of breeding values must be performed using the same level of threonine: lysine ratio in diet the quails will be fed in the production system.

RESUMO Objetivou-se avaliar os efeitos de antioxidantes suplementados às dietas de galos de matrizes pesadas em idade avançada sobre as características reprodutivas. Foram utilizados galos da linhagem Cobb com 47 semanas de idade. Estes foram distribuídos em quatros tratamentos, de acordo com a adição de antioxidante à dieta, sendo: A = grupo controle (sem suplementação antioxidante); B = suplementação de 9ppm de citranaxantina; C = suplementação de 6ppm de cantaxantina e D = 150ppm de vitamina E. Foram avaliadas as características seminais, tais como volume, vigor, motilidade e concentração, a composição de ácidos graxos do sêmen (saturados, insaturados, monoinsaturados, poli-insaturados, ômega 3, ômega 6 e ômega 9) e a correlação entre o peso dos testículos e o peso dos galos. Não foram observados efeitos das substâncias antioxidantes testadas sobre os parâmetros reprodutivos avaliados. Os antioxidantes avaliados não influenciam as respostas reprodutivas de machos reprodutores de matrizes pesadas em idade avançada.

ABSTRACT This study aimed to evaluate the effect of antioxidant supplementation in diets of aging broiler breeder roosters on reproductive characteristics. Cobb roosters were divided into four groups, according to antioxidant addition, where: A = control group (without antioxidant supplementation); B = 9ppm of citranaxanthin; C = 6ppm of canthaxanthin, and D = 150ppm of vitamin E. Seminal characteristics (volume, force, motility and concentration), semen fatty acid composition (saturated, unsaturated, monounsaturated, polyunsaturated, ω3, ω6 and ω9), and the correlation between testis weight with rooster weight were evaluated. No effects of the antioxidants tested were observed. The antioxidants do not influence the reproductive responses of aging male broiler breeder.

Resumo Objetivo Relacionar o estilo de vida e o estado de nutrição das crianças na escola. Método Estudo de correlação, em que participaram 260 crianças vinculadas à clínica ISSSTE, escolhidas aleatoriamente. Utilizou-se um questionário sobre estilos de vida na infância e valorizou-se o peso e o tamanho. Obteve-se a aprovação da chefia de pesquisa do ISSSTE. Na análise de dados aplicou-se estatística descritiva e inferencial. Resultados Participaram 137 meninos e 123 meninas, com idade entre 6-12 anos de idade. Em relação ao estado de nutrição, 5 de cada 10 dos participantes encontram-se em peso normal, 2 de cada 10 com sobrepeso e um de cada 10 com obesidade e baixo peso. Sobre o estilo de vida, 7 de cada 10 dos participantes encontraram-se em risco de baixo peso e 2 de cada 10 em risco moderado. Por outra parte, não se encontrou relação entre o estilo de vida das crianças e o seu estado de nutrição (r s = 0.038, p > 0.05). Adicionalmente, encontrou-se que as crianças com peso normal ou baixo peso têm melhores hábitos de sono do que as crianças com sobrepeso ou obesidade (U = 3.79, p < 0.001). Conclusões Esse estudo mostra indícios de que os aspetos vinculados com a dimensão psicocorporal e com uso do tempo livre deveriam ser indagados com maior profundidade, já que foram os que lançaram dados que situam as crianças na escola em risco moderado e alto.

Abstract Objective To explore the association between lifestyle and nutritional status among school children. Method Correlational study involving 260 children registered at an ISSSTE clinic, who were randomly selected. A questionnaire on childhood lifestyles was used; weight and height were also assessed. A project approval from the ISSSTE research committee was obtained. Descriptive and inferential statistics were used. Results 137 boys and 123 girls with ages between 6 and 12 years old participated. In relation to the nutrition status, five out every ten have a normal weight; two out every ten are over weighted, and one out of every ten is obese or under weighted respectively. In relation to lifestyle, seven out of every ten participants have low risk; and two out of every ten have medium risk. On the other hand a relation between the lifestyle and nutritional status of the children was not found (rs=.038, p > 0.05). Moreover, it was found that children with normal or low weights have better sleep habits in comparison to the children who are over weighted or obese U = 3.79, p < .001). Conclusions This study suggests that issues related to the psychological and corporal dimensions, as well as the use of free time, should be further explored because they showed figures which place children within mid and high risks.

Resumen Objetivo Relacionar el estilo de vida y el estado de nutrición en escolares. Método Estudio correlacional, participaron 260 niños adscritos a la Clínica ISSSTE, elegidos aleatoriamente. Se utilizó el cuestionario sobre estilos de vida en la niñez y se valoró el peso y talla. Se obtuvo la aprobación de la Jefatura de Investigación del ISSSTE. En el análisis de datos se aplicó estadística descriptiva e inferencial. Resultados Participaron 137 niños y 123 niñas, con edades entre 6-12 años. Respecto al estado de nutrición, 5 de cada 10 de los participantes se encuentran en peso normal, 2 de cada 10 en sobrepeso y uno de cada 10 en obesidad y peso bajo. Sobre el estilo de vida 7 de cada 10 de los participantes se encontraron en riesgo bajo y 2 de cada 10 en riesgo mediano. Por otra parte, no se encontró relación entre el estilo de vida de los niños y su estado de nutrición (r s = 0.038, p > 0.05). Adicionalmente, se encontró que los niños con peso normal o bajo peso tienen mejores hábitos de sueño que los niños con sobrepeso u obesidad (U = 3.79, p < 0.001). Conclusiones Este estudio muestra indicios de que los aspectos vinculados con la dimensión psicocorporal y con uso del tiempo libre deberían indagarse a mayor profundidad, ya que fueron los que arrojaron cifras que ubican a los escolares en riesgo medio y alto.

RESUMO Objetivo Avaliar as dimensões do fígado e do baço em pacientes com lúpus eritematoso sistêmico de início pediátrico (LESp) e controles saudáveis. Métodos Foram submetidos a uma ultrassonografia do abdome 30 pacientes com LESp e 30 voluntários saudáveis controle. Foram feitas duas medições do fígado no lobo hepático esquerdo (craniocaudal e anteroposterior) e três no lobo hepático direito (LHD) (craniocaudal posterior [CCP-LHD], craniocaudal anterior e anteroposterior). Foram também avaliadas três medidas das dimensões do baço: longitudinal, transversal e anteroposterior. Foram avaliados dados demográficos, clínicos e laboratoriais, SLEDAI-2K, ECLAM, SLAM e tratamento. Resultados A idade média foi semelhante nos pacientes com LESp e controles (170,31 ± 27,81 vs. 164,15 ± 39,25 meses; p = 0,486). A média da dimensão CCP-LHD foi significativamente maior no grupo LESp em comparação com os controles (13,30 ± 1,85 vs. 12,52 ± 0,93, p = 0,044). Não houve diferenças nos outros parâmetros biométricos do fígado e do baço (p > 0,05). Uma análise especifica realizada apenas nos pacientes com LESp de acordo com a dimensão CCP-LHD ≥ 13,3 cm versus < 13,3 cm mostrou que a mediana do SLEDAI-2K [8 (0-18) vs. 2 (0-8), p = 0,004], ECLAM [4 (0-9) vs. 2 (0-5), p = 0,019] e SLAM [5 (1-13) vs. 2 (0-14), p = 0,016] era significativamente maior em pacientes com maior dimensão CCP-LHD, do mesmo modo que a frequência de nefrite (77% vs. 29%, p = 0,010). As enzimas hepáticas foram semelhantes nos dois grupos (p > 0,05). Foi observada uma correlação positiva entre o SLEDAI-2K e a dimensão CCP-LHD (p = 0,001, r = +0,595). Evidenciou-se uma correlação negativa entre a duração da doença e a dimensão longitudinal do baço (p = 0,031, r = −0,394). Conclusão Os dados levantam a possibilidade de que a atividade da doença pode levar a uma hepatomegalia subclínica e localizada durante o curso da doença. A duração da doença resultou em atrofia do baço em pacientes com LESp.

ABSTRACT Objective To evaluate liver and spleen dimensions in childhood-onset systemic lupus erythematosus (c-SLE) patients and healthy controls. Methods 30 c-SLE patients and 30 healthy control volunteers underwent abdominal ultrasound. The following two liver measurements were performed in left hepatic lobe: craniocaudal and anteroposterior and three in right hepatic lobe (RHL): posterior craniocaudal (PCC-RHL), anterior craniocaudal and anteroposterior. Three spleen dimension measurements were also evaluated: longitudinal, transverse and anteroposterior. Demographic, clinical and laboratorial data, SLEDAI-2K, ECLAM, SLAM and treatment were assessed. Results Mean current age was similar in c-SLE and controls (170.31 ± 27.81 vs. 164.15 ± 39.25months; p = 0.486). The mean of PCC-RHL dimension was significantly higher in c-SLE compared to controls (13.30 ± 1.85 vs. 12.52 ± 0.93, p = 0.044). There were no differences between the other hepatic biometrics and splenic parameters (p > 0.05). Further analysis in c-SLE patients according to PCC-RHL dimension ≥ 13.3 cm versus < 13.3 cm showed that the median of SLEDAI-2K [8(0-18) vs. 2(0-8), p = 0.004], ECLAM [4(0-9) vs. 2(0-5), p = 0.019] and SLAM [5(1-13) vs. 2(0-14), p = 0.016] were significantly higher in patients with higher PCC-RHL dimension, likewise the frequencie of nephritis (77% vs. 29%, p = 0.010). Liver enzymes were similar in both groups (p > 0.05). Positive correlation was observed between SLEDAI-2K and PCC-RHL (p = 0.001, r = +0.595). Negative correlation was evidenced between disease duration and longitudinal dimension of spleen (p = 0.031, r = −0.394). Conclusion Our data raises the possibility that disease activity could lead to a subclinical and localized hepatomegaly during the disease course. Long disease duration resulted to spleen atrophy in c-SLE patients.

Objetivou-se avaliar os efeitos da umidade relativa do ar nas incubadoras (URI) e da idade da matriz leve sobre a eclodibilidade, qualidade dos pintos recém-eclodidos e o desempenho da progênie. Foram incubados 10.840 ovos, sendo os tratamentos definidos pela idade das matrizes Lohmann LSL(r) (26, 41 e 56 semanas) e pelos níveis de URI (48, 56 e 64%). Após a incubação, 1.620 pintos fêmeas foram alojados em galpão experimental convencional, de acordo com os tratamentos, até as 12 semanas de idade. Para a avaliação de eclodibilidade sobre ovos férteis e fêmeas refugos, o delineamento experimental foi em blocos ao acaso no arranjo em parcelas subdivididas. A parcela foi a URI (três níveis) e a subparcela foi a idade da matriz (três idades). Os blocos foram constituídos pelos andares dos carrinhos de incubação, em que cada andar continha uma repetição de cada idade da matriz. Os nove tratamentos foram constituídos por 14 repetições cada. As repetições foram bandejas de incubação, constituindo 1.204 ovos por tratamento. Para a avaliação do desempenho das frangas, o delineamento experimental foi inteiramente ao acaso, constituído por um esquema fatorial 3x3 (três níveis de URI e três idades), com seis repetições de 30 aves em cada tratamento. A eclodibilidade sobre ovos férteis foi maior (P<0,05) com a utilização de 56% de umidade relativa do ar durante a incubação, independentemente da idade da matriz; e ovos de matrizes mais velhas (56 semanas) apresentaram eclodibilidade menor (P<0,05), independentemente do nível de umidade relativa do ar no interior das incubadoras. Não foram constatados efeitos da idade da matriz e URI sobre o percentual de pintos refugos. A URI ou a idade da matriz leve não afetam o desempenho da progênie.

The aim of this study was to evaluate the effects of relative humidity in incubators (RHI) and breeder hen age on hatchability, hatchling quality and performance of progeny. 10,840 eggs were incubated, and the treatments were defined by the Lohmann LSL(r) breeder age (26, 41 and 56 weeks) and the levels of RHI (48, 56 and 64%). After incubation, 1,620 female chicks were raised in a conventional experimental shed, according to the treatments, until 12 weeks of age. To assess the hatchability of fertile eggs and unviable female chicks, the experimental design was randomized blocks in split plots design. The plot factor was the RHI and the subplot was the age of the breeder hens. The blocks were made by the floors of the incubation carriages, where each floor contained a repetition of each breeder age. The nine treatments consisted of 14 repetitions each. The repetitions were trays of incubation, with 1,204 eggs per treatment. To evaluate the performance of the progeny, the experimental design was completely randomized, consisting of a 3x3 factorial scheme with six replicates of 30 birds in each treatment. Hatchability of fertile eggs was higher (P<0.05) with the use of 56% relative humidity during incubation, regardless of breeder age, and the eggs from the oldest breeder hens (56 weeks) had lower hatchability (P<0.05), regardless of the level of relative humidity inside the incubator. No effects of breeder age and RHI on the percentage of unviable chicks were observed. The RHI or the age of the breeder hen does not affect the performance of progeny.