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RESUMO Objetivou-se avaliar diferentes planos nutricionais para vacas F1 Holandês/Zebu mantidas em pasto diferido de capim-braquiária sobre a produção e a composição do leite e no ganho em peso corporal. Foram utilizadas 36 vacas F1 Holandês/Zebu com período médio de lactação de 267 ± 10 dias, peso corporal médio de 548 ± 19kg, seguindo o delineamento inteiramente ao acaso, em esquema fatorial 4 x 5, sendo quatro estratégias de alimentação e cinco dias em avaliação. Os tratamentos consistiram de quatro planos nutricionais: pasto diferida como fonte de forragem sem suplementação (PDSS); pastagem diferida como fonte de forragem com oferta de suplemento de proteico (PDCS); pasto diferido suplementado com 15 quilos de silagem de milho (base natural) + 1.200 gramas de suplemento proteico (PDSP) e silagem de milho (ad libitum) + 700 gramas de suplemento de proteína (SMP). Verificou-se que as vacas alimentadas com SMP apresentaram produção de leite 26,06% superior aos demais planos nutricionais (média de 11,46kg/dia). Vacas F1 Holandês/Zebu tratadas em pastagem diferida de maneira tradicional, suplementada com proteína, mantêm o rendimento de leite.

ABSTRACT The objective of this study was to evaluate different supplementation strategies concentrated to F1 Holstein x Zebu lactating cows managed in deferred signal grass pasture on the yield and composition of milk and body weight gain. Thirty six F1 Holstein x Zebu cows with an average lactation period of 267 ± 10 days, mean body weight of 548 ± 19kg were used following a completely randomized design in a 4 x 5 factorial scheme, being four feeding strategies and five days under evaluation. The treatments consisted of four nutritional strategies: deferred pasture as a source of roughage without supplementation (PDSS); deferred pasture as a source of roughage with protein supplement offer (PDCS); deferred pasture supplemented with 15 kilos of corn silage (natural base) + 1,200 grams of protein supplement (PDSP) and corn silage (ad libitum) + 700 grams of protein supplement (SMP). There was no interaction (P> 0.05) between the nutritional plans and days under evaluation for any of the variables. It was found that cows fed SMP showed milk production 26.06% higher than the other nutritional plans (mean of 11.46kg/day). F1 Holstein/Zebu cows handled in deferred pasture in a traditional way supplemented with protein maintains milk yield.

https://doi.org/10.1590/1678-4162-12090 241 downloads

Depression and anxiety disorders in patients with multiple sclerosis: association with neurodegeneration and neurofilaments
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Tauil, C.B.

; Rocha-Lima, A.D.

; Ferrari, B.B.

; Silva, F.M. da

; Machado, L.A.

; Ramari, C.

; Brandão, C.O.

; Santos, L.M.B. dos

; Santos-Neto, L.L. dos

Brazilian Journal of Medical and Biological Research

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2021, Volume 54 N. 3 elocation e10428

Abstract: En | Text: En | PDF: En

There is increasing evidence that neurofilament light chain (NF-L) can be considered as a biomarker for neuro-axonal damage. This polypeptide can be released into the cerebrospinal fluid (CSF) and the blood, where it can be quantified. The concentration of NF-L is elevated in patients with multiple sclerosis (MS) and psychiatric disorders. We aimed to investigate the NF-L levels in the CSF from treated MS patients and the relationship with depression or anxiety. The study involved three groups: control group (individuals without inflammation), the relapse-remitting multiple sclerosis (RRMS)-untreated group, and the RRMS-Fingo group (RRMS patients who were treated with fingolimod). MS disability was assessed by the Expanded Disability Status Scale, and depression and anxiety were evaluated by a neuropsychologist, using the Hospital Anxiety and Depression Scale, the Beck Depression Inventory-II, and the Beck Anxiety Inventory. Individual CSF samples were collected to measure NF-L levels. The results of the statistical analysis on levels of NF-L in the CSF of control subjects, RRMS-untreated patients, and RRMS-Fingo patients were significant. The relationship between depression and anxiety in RRMS-Fingo patients and NF-L levels was not statistically significant. In conclusion, MS events such as anxiety and depression appear to contribute to the onset of clinical relapses, subclinical cases, and neurodegeneration.

https://doi.org/10.1590/1414-431x202010428 924 downloads

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle
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Antunes, M.M.

; Godoy, G.

; de Almeida-Souza, C.B.

; da Rocha, B.A.

; da Silva-Santi, L.G.

; Masi, L.N.

; Carbonera, F.

; Visentainer, J.V.

; Curi, R.

; Bazotte, R.B.

Brazilian Journal of Medical and Biological Research

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2020, Volume 53 N. 3 elocation e9039

Abstract: En | Text: En | PDF: En

We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), Δ-6 desaturase (D6D), elongase 6, and de novo lipogenesis (DNL) were estimated. As for indicators of the inflammatory tissue state, we measured myeloperoxidase (MPO) activity and gene expression of F4/80, tumor necrosis factor-α (TNF-α), interleukin (IL)-4, IL-6, and IL-10. The HCD led to a lower deposition of SFA, MUFA, n-3 PUFA, and n-6 PUFA compared to HFD. However, the HCD increased arachidonic acid levels, SFA/n-3 PUFA ratio, DNL, SCD-1, D6D, and MPO activities, and expression of IL-6, contrasting with the general idea that increased lipid deposition is associated with more intense inflammation. The HCD was more potent to induce skeletal muscle inflammation than the HFD, regardless of the lower lipid accumulation.

https://doi.org/10.1590/1414-431x20199039 1930 downloads

Perfil de los pacientes rehabilitados por medio de prótesis atendidos en servicio público de salud en Ribeirão Preto, Brasil
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de Andrade Lima Chaves, C.

; Alves Gomes, E.

; Faria, T.

; Rocha Correa da Silva, S.

; de Souza Filho, C.B.

; Borie, E.

; Corrêa Silva Sousa, Y.T.

Avances en Odontoestomatología

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dec 2015, Volume 31 N. 6 Pages 347 - 353

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The prevalence of gender and age related pattern of the different types of prosthetic restorations are important variables to be estimated. The aim of this study was to evaluate the profile of age and gender in patients rehabilitated with dental prostheses on School of Dentistry during five years. Were evaluated 638 records of patients who received fixed prosthesis (FP), removable partial (RP) or total dentures (T). Rehabilitated patients with RP had their prostheses classified into dental-mucous-supported (DM) or tooth-supported (D). To analyze the results, patients were divided into 2 groups: A) >50 years or B) <50 years. Data were statistically analyzed by Fisher's exact test or chi Square (p<0.05). No statistical significance relation to age and types of prostheses was observed. On RP, 47% was D and 53% DM, there was no statistically significant difference. It was possible to conclude that the rehabilitation of greater number of the teeth increases with age and is prevalent in all periods of adulthood, especially in women.

La prevalencia de sexo y el padrón de edad relacionado a los diferentes tipos de rehabilitaciones protésicas son variables importantes que deben ser estudiadas. De esta manera, el objetivo de este estudio fue evaluar el perfil de edad y género en pacientes rehabilitados con prótesis dentales en un curso de Odontología durante un periodo de cinco años. Fueron evaluados 638 fichas de pacientes que recibieron prótesis parcial fija (PPF), prótesis parcial removible (PPR) y prótesis total (PT). Las prótesis de los pacientes rehabilitados con PPR fueron clasificadas en dentomucosoportada (DM) o dentosoportadas (D). Para el análisis de los resultados, los pacientes fueron divididos en 2 grupos: A) >50 años y B) <50 años. Los datos fueron analizados estadísticamente por el test exacto de Fisher o chi-cuadrado (p<0,05). No se observaron diferencias significativas en relación a la edad y tipo de prótesis. Para la PPF 74% fueron <50 años, para la PPR 52%, >50 años y para la PT 92%, >50 años. En todas las prótesis, existió una mayor prevalencia del género femenino. Con relación a la clasificación de las PPRs, 47% fueron D y 53% DM, sin identificar diferencias estadísticamente significativas. De esta manera se puede concluir que la necesidad de rehabilitación de un mayor numero de elementos dentales aumenta con la edad, siendo prevalente en todos los periodos de la vida adulta, principalmente, en mujeres.

https://doi.org/10.4321/S0213-12852015000600002 957 downloads

Isolation, characterization and evaluation of antimicrobial and cytotoxic activity of estragole, obtained from the essential oil of croton zehntneri (euphorbiaceae)
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ANDRADE, THALLITA C.B.

; LIMA, SIDNEY G. DE

; FREITAS, RIVELILSON M.

; ROCHA, MÁRCIO S.

; ISLAM, TOREQUL

; SILVA, TERESINHA G. DA

; MILITÃO, GARDENIA C.G.

Anais da Academia Brasileira de Ciências

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mar 2015, Volume 87 N. 1 Pages 173 - 182

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Croton zehntneri (Euphorbiaceae) é uma planta aromática nativa da região Nordeste do Brasil. O monoterpenóide Estragol (ESL) foi isolado por métodos cromatográficos clássicos a partir do óleo essencial (OE) das folhas de C. zehntneri e caracterizado por CG-DIC e CG-EM, tendo seu potencial antimicrobiano e citotóxico avaliado. A análise do OE permitiu a identificação de 100% dos constituintes integrados, cujo rendimento foi cerca de 1.8%. Os principais componentes identificados foram: eucaliptol, estragol (84.7%) e espatulenol. A dosagem de 50 μg/disco de ESL apresentou halos de inibição bastante expressivos frente a bactérias gram positiva e fungos. O ESL apresentou toxicidade frente a Artemia salina com CL50 e CL90 de 4,54 e 8,47 μg mL-1. Entretanto, nos testes de inibição tumoral (células humanas), não houve inibição satisfatória em nenhuma das linhagens de células humanas cancerígenas (MCF-7, HEP-2 e NCIH292).

Croton zehntneri (Euphorbiaceae) is a native aromatic plant from Northeast region of Brazil. The monoterpenoid estragole (ESL) has been isolated by classical chromatographic methods from the essential oil (EO) of C. zehnteneri leaves and characterized by GC-FID and GC-MS, its antimicrobial and cytotoxic potentials being assessed. The analysis of the EO enabled the identification of 100% of the integrated constituents, of which yield was about 1.8%. The main components identified were: eucalyptol, estragole (84.7%) and spathulenol. The dosage of 50 μg/disk of ESL presented fairly significant zones of inhibition against Gram-positive bacteria and fungi. The ESL presented toxicity against Artemia salina with LC50 and LC90 of 4,54 and 8,47 μg mL-1. However, in tumor inhibition assays (human cells), there were no rewarding inhibition in any of the human cancer cell lines (MCF-7, HEP-2 and NCI-H292).

https://doi.org/10.1590/0001-3765201520140111 3034 downloads

Non-nutritive sucking evaluation in preterm newborns and the start of oral feeding: a multicenter study
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Neiva, Flávia C.B.

; Leone, Cléa R.

; Leone, Claudio

; Siqueira, Lisiane L.

; Uema, Kátia Akiko

; Evangelista, Daiana

; Delgado, Susana

; Rocha, Adriana

; Buhler, Karina Bernardis

Clinics

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jun 2014, Volume 69 N. 6 Pages 393 - 397

Abstract: En | Text: En | PDF: En

OBJECTIVES:The assessment of early sucking by preterm infants provides information on the ability of these infants to efficiently and safely receive nutrients via an oral route (oral feeding). To analyze the application and reliability of an instrument in assessing non-nutritive sucking that indicates a capacity for oral feeding in the routine care of different neonatal units.METHODS:A multicenter, prospective cohort study was conducted in seven neonatal units. A non-nutritive sucking assessment with a formulary validated by Neiva et al (2008) (variables evaluated: rooting reaction; easy initiation of sucking; labial sealing; tongue central groove; peristaltic tongue movements; jaw raising and lowering movements; labial, tongue and jaw coordination; sucking strength; sucking rhythm; bites; excessive jaw excursion; stress signals) was applied to 199 pre-term newborns, who had a chronological age ≥ 2 days and were clinically stable. These infants were divided into two groups based on their corrected gestational age at the first assessment, as follows: Group I-infants with a gestational age ≤ 33 weeks and Group II-infants with a gestational age between 34 and 36 6/7 weeks.RESULTS:The mean gestational age was 31.66±2 weeks, and the mean birth weight was 1494 ± 373 g. The mean scores on the non-nutritive sucking assessment were 46 ± 25 in Group I and 49 ± 24 in Group II. The beginning of oral feeding was successful in 43 (67.2%) infants in Group I and 64 (81%) infants in Group II (p= 0.089).CONCLUSION:The method identified preterm infants who were able to feed orally based on 33 points in the non-nutritive sucking assessment and a corrected gestational age of 32 weeks or more. The corrected gestational age was the most important factor in predicting the success of oral feeding.

https://doi.org/10.6061/clinics/2014(06)05 7638 downloads

Comparison of different protocols for the extraction of microbial DNA from reef corals
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Santos, H.F.

; Carmo, F.L.

; Leite, D.C.A.

; Jesus, H.E.

; De Carvalho Maalouf, P.

; Almeida, C.

; Soriano, A.U.

; Altomari, D.

; Suhett, L.

; Vólaro, V.

; Valoni, E.

; Francisco, M.

; Vieira, J.

; Rocha, R.

; Sardinha, B.L.

; Mendes, L.B.

; João, R.R.

; Lacava, B.

; Jesus, R.F.

; Sebastian, G.V.

; Pessoa, A.

; van Elsas, J.D.

; Rezende, R.P.

; Pires, D.O.

; Duarte, G.

; Castro, C.B.

; Rosado, A.S.

; Peixoto, R.S.

Brazilian Journal of Microbiology

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jun 2012, Volume 43 N. 2 Pages 517 - 527

Abstract: En | Text: En | PDF: En

This study aimed to test different protocols for the extraction of microbial DNA from the coral Mussismilia harttii. Four different commercial kits were tested, three of them based on methods for DNA extraction from soil (FastDNA SPIN Kit for soil, MP Bio, PowerSoil DNA Isolation Kit, MoBio, and ZR Soil Microbe DNA Kit, Zymo Research) and one kit for DNA extraction from plants (UltraClean Plant DNA Isolation Kit, MoBio). Five polyps of the same colony of M. harttii were macerated and aliquots were submitted to DNA extraction by the different kits. After extraction, the DNA was quantified and PCR-DGGE was used to study the molecular fingerprint of Bacteria and Eukarya. Among the four kits tested, the ZR Soil Microbe DNA Kit was the most efficient with respect to the amount of DNA extracted, yielding about three times more DNA than the other kits. Also, we observed a higher number and intensities of DGGE bands for both Bacteria and Eukarya with the same kit. Considering these results, we suggested that the ZR Soil Microbe DNA Kit is the best adapted for the study of the microbial communities of corals.

24569 downloads Cited 1 time in SciELO

Traffic modeling in PLC networks using a Markov Fluid Model with autocorrelation function fitting
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Rocha, F.G.C.

; Santos, C.B.

; Vieira, F.H.T.

TEMA (São Carlos)

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dec 2011, Volume 12 N. 3 Pages 233 - 243

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste artigo, apresentamos uma análise do tráfego VoIP (Voice over IP) e de transferência de dados usando uma rede PLC (PowerLine Communications). Propomos um modelo baseado em MMFM (Markov Modulated Fluid Models) para tráfego de dados e VoIP em redes PLC. Simulações e comparações foram realizadas para verificar a eficiência do modelo de tráfego proposto em relação aos modelos de Poisson e MMPP (Markov Modulated Poisson Process).

In this paper, we present an analysis of VoIP (Voice over IP) traffic and data transfer using PLC (PowerLine Communications) network. We propose a model based on MMFM (Markov Modulated Fluid Models) for data and VoIP traffic in PLC networks. Simulations and comparisons were carried out to verify the efficiency of the proposed traffic model over the Poisson and MMPP (Markov Modulated Poisson Process) models.

https://doi.org/10.5540/tema.2011.012.03.0233 2428 downloads Cited 1 time in SciELO

Occurrence and control of Chilodonella spp. in pejerrey Odontesthes bonariensis
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Fernandes, J. Morato

; Portelinha, M.K.

; Rocha, C.B.

; Pouey, J.L.O.F.

; Piedras, S.R.N.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2011, Volume 63 N. 3 Pages 788 - 790

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente trabalho teve por objetivo relatar a ocorrência e controle de infecção parasitária por Chilodonella spp. em alevinos de peixe-rei (O. bonariensis). A Chilodonella spp. é um ciliado que parasita tegumento e brânquias de peixes, podendo ocasionar grande mortalidade. A infecção foi identificada a partir da observação comportamental de alevinos criados em caixas de polietileno com capacidade de 1000 litros, mantidas em circuito fechado com um biofiltro e alimentados com ração e zooplancton selvagem. A contaminação foi constatada devido à redução do apetite, perda do equilíbrio e alteração na coloração dos peixes, tendo como causa provável, a elevação dos níveis de amônia não ionizável, levando a uma diminuição da imunidade. Como tratamento foi utilizado uma concentração salina de 10g/L, durante 30 minutos, com êxito para o controle deste parasito.

2166 downloads

10.

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.
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Farias, Davi F.

; Cavalheiro, Mariana G.

; Viana, Martônio P.

; Queiroz, Vanessa A.

; Rocha-Bezerra, Lady C.B.

; Vasconcelos, Ilka M.

; Morais, Selene M.

; Carvalho, Ana F.U.

Anais da Academia Brasileira de Ciências

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sep 2010, Volume 82 N. 3 Pages 585 - 594

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este trabalho objetivou avaliar a toxicidade dos extratos aquosos de sementes de 15 espécies de leguminosas contra larvas de Aedes aegypti. Foi realizada uma caracterização química e bioquímica parcial dos extratos aquosos e a avaliação da toxicidade aguda em camundongos. Os extratos de Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum e Piptadenia moniliformis causaram 100% de mortalidade depois de 1 a 3 h de exposição e mostraram valores de CL50 e CL90 entre 0,43 = 0,01 e 9,06 ± 0,12 e entre 0,71 = 0,02 e 13,03 = 0,15 mg/mL, respectivamente. Dentre os constituintes do metabolismo secundário, os extratos das sementes apresentaram taninos, fenóis, flavonas, flavonóis, xantonas, saponinas e alcalóides. Os extratos apresentaram alto teor de proteínas solúveis (0,98 to 7,71 mg/mL), lectina (32 to 256 UH/mL) e inibidor de tripsina (3,64 ± 0,43 to 26,19 = 0,05 gIT/kg de farinha). O perfil eletroforéticomostrou uma grande diversidade de proteínas, muitas dasquais já descritas como inseticidas. Os extratos mostraram baixa toxicidade ao camundongo (DL50 > 0,15 = 0,01 g/kg peso corporal), porém apesar desses resultados promissores, estudos posteriores são necessários para compreender a toxicidade desses extratos e de seus constituintes do metabolismo primário e secundário sobre Ae. aegypti.

This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC50 and LC90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high soluble proteins content (0.98 to 7.71 mg/mL), lectin (32 to 256 HU/mL) and trypsin inhibitory activity (3.64 = 0.43 to 26.19 = 0.05 gIT/kg of flour) The electrophoretic profiles showed a great diversity of protein bands, many of which already described as insecticide proteins. The extracts showed low toxicity to mice (LD50 > 0.15 = 0.01 g/kg body weight), but despite these promising results, further studies are necessary to understand the toxicity of these extracts and their constituentsfrom primary and secondary metabolism upon Ae. aegypti.

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11.

Neuroimaging in Parkinsonism: a study with magnetic resonance and spectroscopy as tools in the differential diagnosis
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Vasconcellos, Luiz Felipe Rocha

; Novis, Sergio A. Pereira

; Moreira, Denise Madeira

; Rosso, Ana Lucia Z.

; Leite, Ana Claudia C.B.

Arquivos de Neuro-Psiquiatria

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mar 2009, Volume 67 N. 1 Pages 1 - 6

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O diagnóstico diferencial do parkinsonismo baseado em parâmetros clínicos pode ser difícil. Alguns exames complementares podem ser úteis, especialmente a ressonância magnética, um método não invasivo, de menor custo quando comparado a tomografia por emissão de pósitrons, proporcionando uma análise anatômica satisfatória. A ressonância por espectroscopia analisa o metabolismo cerebral, com resultados variáveis na literatura no estudo das síndromes parkinsonianas. Selecionamos 40 indivíduos para realização de ressonância magnética e espectroscopia, sendo 12 com doença de Parkinson, 11 com paralisia supranuclear progressiva, 7 com atrofia de múltiplos sistemas tipo parkinsoniana e 10 indivíduos sem manifestações neurológicas ou psiquiátricas (grupo controle). As escalas clínicas analisadas foram a de Hoenh e Yahr, unified Parkinson's disease rating scale e o mini-exame do estado mental. Os resultados encontrados revelaram que pacientes com doença de Parkinson e controle apresentavam em geral o mesmo aspecto por imagem enquanto os grupos paralisia supranuclear progressiva e atrofia de múltiplos sistemas com anormalidades, havendo significância estatística em algumas variáveis. A ressonância magnética e a espectroscopia podem ser úteis no diagnóstico diferencial do parkinsonismo.

The differential diagnosis of Parkinsonism based on clinical features, sometimes may be difficult. Diagnostic tests in these cases might be useful, especially magnetic resonance imaging, a noninvasive exam, not as expensive as positron emission tomography, and provides a good basis for anatomical analysis. The magnetic resonance spectroscopy analyzes cerebral metabolism, yielding inconsistent results in parkinsonian disorders. We selected 40 individuals for magnetic resonance imaging and spectroscopy analysis, 12 with Parkinson's disease, 11 with progressive supranuclear palsy, 7 with multiple system atrophy (parkinsonian type), and 10 individuals without any psychiatric or neurological disorders (controls). Clinical scales included Hoenh and Yahr, unified Parkinson's disease rating scale and mini mental status examination. The results showed that patients with Parkinson's disease and controls presented the same aspects on neuroimaging, with few or absence of abnormalities, and supranuclear progressive palsy and multiple system atrophy showed abnormalities, some of which statistically significant. Thus, magnetic resonance imaging and spectroscopy could be useful as a tool in differential diagnosis of Parkinsonism.

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