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au:Ribeiro, Carla M.

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Detection of alloimmunization in Glanzmann Thrombasthenia and Bernard-Soulier Syndrome: Data from a Brazilian Center
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Gabe, Caroline

; Ziza, Karen Chinoca

; Durazzo, Natália

; Pagani, Flavia M.

; Oliveira, Valéria Brito

; Conrado, Marina-C.A.V.

; Dezan, Marcia R.

; Mendrone Jr., Alfredo

; Villaça, Paula Ribeiro

; Dinardo, Carla Luana

; Rocha, Vanderson

Hematology, Transfusion and Cell Therapy

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jul 2023, Volume 45 Suppl. 2 Pages S101 - S107

Abstract: En | Text: En | PDF: En

ABSTRACT Introduction: The Glanzmann Thrombasthenia (GT) and Bernard-Soulier Syndrome (BSS) are rare hereditary disorders of platelet function. Their treatment often requires platelet transfusion, which can lead to the development of alloantibodies. Objective: In this study, we aim to develop a strategy for alloantibody detection and to describe the frequency of alloimmunization in a patient population from a single center in southeastern Brazil. Methods: Samples from patients with GT or BSS were tested using the Platelet Immunofluorescence Test (PIFT). If a positive result was obtained, a confirmatory step using the Monoclonal Antibody Immobilization of Platelet Antigens (MAIPA) and Luminex bead-based platelet assay (PAKLx) was executed. Main results: Among 11 patients with GT, we detected the presence of alloantibodies in 5 using PIFT, with confirmation through MAIPA and PAKLx in 2 (1 anti-HLA and 1 anti-HPA), resulting in a frequency of 18.1%. Among 4 patients with BSS, PIFT was positive in 3, with confirmation by MAIPA and PAKLx in 1 (anti-HLA), showing a frequency of 25%. The two patients with anti-HLA antibodies exhibited a panel reactive antibody (PRA-HLA) testing greater than 97%. Conclusion: Our study highlights the importance of identifying platelet alloimmunization in this patient population. The proposed algorithm for platelet alloantibodies detection allows resource optimization.

https://doi.org/10.1016/j.htct.2022.06.005

Anatomy of the lower hypogastric plexus applied to endometriosis: a narrative review
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Ribeiro-Julio, Gisele Silva

; Pereira, Jorge Alves

; Ribeiro, Eduardo

; Gallo, Carla M.

; Favorito, Luciano A.

International braz j urol

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jun 2023, Volume 49 N. 3 Pages 299 - 306

Abstract: En | Text: En | PDF: En

ABSTRACT Objective The objective of the present study is to evaluate the anatomy of the inferior hypogastric plexus, correlating it with urological pathologies, imaging exams and surgeries of the female pelvis, especially for treatment of endometriosis. Material and Methods We carried out a review about the anatomy of the inferior hypogastric plexus in the female pelvis. We analyzed papers published in the past 20 years in the databases of Pubmed, Embase and Scielo, and we included only papers in English and excluded case reports, editorials, and opinions of specialists. We also studied two human fixed female corpses and microsurgical dissection material with a stereoscopic magnifying glass with 2.5x magnification. Results Classical anatomical studies provide few details of the morphology of the inferior hypogastric plexus (IHP) or the location and nature of the associated nerves. The fusion of pelvic splanchnic nerves, sacral splanchnic nerves, and superior hypogastric plexus together with visceral afferent fibers form the IHP. The surgeon’s precise knowledge of the anatomical relationship between the hypogastric nerve and the uterosacral ligament is essential to reduce the risk of complications and postoperative morbidity of patients surgically treated for deep infiltrative endometriosis involving the uterosacral ligament. Conclusion Accurate knowledge of the innervation of the female pelvis is of fundamental importance for prevention of possible injuries and voiding dysfunctions as well as the evacuation mechanism in the postoperative period. Imaging exams such as nuclear magnetic resonance are interesting tools for more accurate visualization of the distribution of the hypogastric plexus in the female pelvis.

https://doi.org/10.1590/s1677-5538.ibju.2022.9980

Immunopathological evaluation of oral squamous cell carcinomas: a case series
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Souza, Vinícius G. de

; Santos, Damilys J.

; Araújo, Tatiana S.

; Silva, Ana G.

; Ribeiro, Rosy I.

; Loyola, Adriano M.

; Cardoso, Sérgio V.

; Siqueira-Miranda, Carla S.

; Cardoso, Ludimila P.

Gaceta mexicana de oncología

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2023, Volume 22 Suppl. 1 Pages 100 - 104

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen La evaluación de la expresión del ligando 1 de muerte programada (PD-L1) es un criterio de elegibilidad para la inmunoterapia anti-PD-L1 en el carcinoma oral de células escamosas (COCE). También deben evaluarse otros componentes del microambiente tisular. Aquí se informaron cuatro casos de carcinoma de células escamosas orales. Se realizaron análisis histopatológicos del grado tumoral y TIL (infiltrado inflamatorio asociado a tumor) e inmunohistoquímica para CD4, CD8 y PD-L1. Los casos se clasificaron según el microambiente tisular en resistencia inmunitaria adaptativa, ignorancia inmunológica, tolerancia o inducción intrínseca. Este análisis destaca la importancia de comprender el microambiente inmunológico que puede presentar el COCE para la identificación de dianas terapéuticas más eficaces contra la enfermedad.

Abstract The evaluation of the expression of programmed cell death protein (PD-L1) is an eligibility criterion for anti-PD-L1 immunotherapy in oral squamous cell carcinoma (OSCC). Other components of tissue microenvironment should also be evaluated. Here, four cases of oral squamous cells carcinoma were reported. Histopathological analysis of tumor grade and TILs and immunohistochemistry for CD4, CD8, and PD-L1 was done. The cases were classified according to the tissue microenvironment in adaptive immune resistance, immunological ignorance, tolerance, or intrinsic induction. This analysis highlights the importance of understanding the immunological microenvironment that the OSCC can present for the identification of more effective therapeutic targets against the disease.

https://doi.org/10.24875/j.gamo.22000145

Posicionamento sobre a Saúde Cardiovascular nas Mulheres – 2022
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Oliveira, Glaucia Maria Moraes de

; Almeida, Maria Cristina Costa de

; Marques-Santos, Celi

; Costa, Maria Elizabeth Navegantes Caetano

; Carvalho, Regina Coeli Marques de

; Freire, Cláudia Maria Vilas

; Magalhães, Lucelia Batista Neves Cunha

; Hajjar, Ludhmila Abrahão

; Rivera, Maria Alayde Mendonça

; Castro, Marildes Luiza de

; Avila, Walkiria Samuel

; Lucena, Alexandre Jorge Gomes de

; Brandão, Andréa Araujo

; Macedo, Ariane Vieira Scarlatelli

; Lantieri, Carla Janice Baister

; Polanczyk, Carisi Anne

; Albuquerque, Carlos Japhet da Matta

; Born, Daniel

; Falcheto, Eduardo Belisário

; Bragança, Érika Olivier Vilela

; Braga, Fabiana Goulart Marcondes

; Colombo, Fernanda M. Consolim

; Jatene, Ieda Biscegli

; Costa, Isabela Bispo Santos da Silva

; Rivera, Ivan Romero

; Scholz, Jaqueline Ribeiro

; Melo Filho, José Xavier de

; Santos, Magaly Arrais dos

; Izar, Maria Cristina de Oliveira

; Azevedo, Maria Fátima

; Moura, Maria Sanali

; Campos, Milena dos Santos Barros

; Souza, Olga Ferreira de

; Medeiros, Orlando Otávio de

; Silva, Sheyla Cristina Tonheiro Ferro da

; Rizk, Stéphanie Itala

; Rodrigues, Thais de Carvalho Vieira

; Salim, Thaís Rocha

; Lemke, Viviana de Mello Guzzo

Arquivos Brasileiros de Cardiologia

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nov 2022, Volume 119 N. 5 Pages 815 - 882

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20220734

WSOC and Its Relationship with BC, Levoglucosan and Transition Metals in the PM2.5 of an Urban Area in the Amazon
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Fernandes, Karenn S.

; Santos, Erickson O. dos

; Batista, Carla E.

; Ribeiro, Igor O.

; Piracelli, Victor P.

; Solci, Maria Cristina

; Duvoisin Jr., Sergio

; Martin, Scot T.

; Souza, Rodrigo A. F.

; Machado, Cristine M. D.

Journal of the Brazilian Chemical Society

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jun 2022, Volume 33 N. 6 Pages 570 - 581

Abstract: En | Text: En | PDF: En

Water-soluble organic carbon (WSOC) makes up a large mass fraction of the organic carbon in the aerosol and can influence important cloud processes in the atmosphere. The capacity of WSOC to form metallic complexes with transition metals is well known; however, its influence on the aerosol of urban areas in the Amazon region is not very well known. In this study, we investigated the relationship between WSOC, black carbon (BC), levoglucosan (LEV) and transition metals (Fe, Cu and Mn) present in the PM2.5 (particles with a diameter smaller than 2.5 µm) of an urban environment during the dry season in the central Amazon. Oxalic acid (C2) was used to identify the influence of transition metals on WSOC. The mean mass concentration value of the PM2.5 was 14.72 μg m-3 (2.11-31.68 μg m-3). The WSOC made up 58.34% of the PM2.5 mass, followed by BC (20.28%), and LEV (2.62%). The WSOC showed significant correlation with the transition metals analyzed (> 0.56), especially Mn with C2 (linear coefficient (R2) = 0.74). A multiple linear regression with WSOC, BC and LEV showed a strong linear correlation between them (R2 = 0.86), indicating the influence of biomass burning and vehicle traffic on the organic aerosol.

https://doi.org/10.21577/0103-5053.20220011

Growth, leaf gas exchange and mycorrhizal colonization of three medicinal species submitted to different irradiance levels
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Barazetti, Viviane Maria

; Gross, Eduardo

; Sodré, George Andrade

; Dalmolin, Ândrea Carla

; Costa, Larissa Corrêa do Bomfim

; Ribeiro, Miguel Antônio Quinteiro

Ciência Rural

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2022, Volume 52 N. 3 elocation e20200633

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste trabalho foi avaliar o crescimento, as trocas gasosas foliares e a colonização por fungos micorrízicos arbusculares em três espécies de plantas medicinais, sob diferentes intensidades de irradiância. Fridericia chica (Bonpl.) L.G. Ohmmann, Mikania laevigata Sch.Bip. ex Baker e Varronia curassavica Jacq. foram propagadas por estaquia e cultivadas por 120 dias em ambientes artificialmente sombreados, utilizando telas do tipo sombrite, em quatro níveis de irradiância, 100%, 70%, 50% e 30%. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 3 x 4 (três espécies de plantas e quatro níveis de irradiação) com sete repetições. As três espécies medicinais avaliadas apresentaram maiores taxas de assimilação líquida, crescimento em massa e colonização radicular por fungos micorrízicos arbusculares quando expostas a ambientes com 70% de disponibilidade de luz. Em relação às respostas fisiológicas, V. curassavica apresentou maior taxa fotossintética, condutância estomática e transpiração quando submetidas a 70% de irradiância, podendo ser cultivada em ambientes mais abertos e com maiores níveis de radiação. Por outro lado, F. chica e M. laevigata apresentaram características de plantas tolerantes à sombra. Os resultados obtidos na fase inicial de crescimento podem ser utilizados como um indicador para recomendar o ambiente de plantio dessas três espécies medicinais em sistemas agroflorestais.

ABSTRACT: This study o evaluated growth, leaf gas exchange and arbuscular mycorrhizal fungi root colonization in three medicinal plant species under different irradiance intensities. Fridericia chica (Bonpl.) L.G.Lohmann, Mikania laevigata Sch.Bip. ex Baker and Varronia curassavica Jacq. were propagated by cutting and cultivated for 120 days in artificially shaded environments using black shade-type screens, obtaining four irradiance levels: 100%, 70%, 50% and 30%. The experimental design was completely randomized in a 3 x 4 factorial scheme (three plant species and four irradiation levels) with seven replicates. The three medicinal species showed higher liquid assimilation, mass growth and arbuscular mycorrhizal fungi root colonization rates when exposed to environments with 70% light availability. In relation to physiological responses, V. curassavica presented higher photosynthetic rate, stomatal conductance and transpiration when submitted to 70% irradiance, being able to be cultivated in more open environments with higher irradiation levels. Conversely F. chica and M. laevigata presented shade tolerance characteristics. At the initial growth phase, the results obtained can be used as indicators to recommend the ideal cultivation environment for these species in agroforestry systems.

https://doi.org/10.1590/0103-8478cr20200633 95 downloads

Saúde Cardiovascular e Fibrilação ou Flutter Atrial: Um Estudo Transversal do ELSA-Brasil
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Santos, Itamar S.

; Lotufo, Paulo A.

; Goulart, Alessandra C.

; Brant, Luisa C. C.

; Pinto Filho, Marcelo M

; Pereira, Alexandre C.

; Barreto, Sandhi M.

; Ribeiro, Antonio L. P.

; Thomas, G Neil

; Lip, Gregory Y. H.

; Bensenor, Isabela M.

; Arasalingam, Ajini

; Beane, Abi

; Bensenor, Isabela M

; Brocklehurst, Peter

; Cheng, Kar Keung

; El-Bouri, Wahbi

; Feng, Mei

; Goulart, Alessandra C

; Greenfield, Sheila

; Guo, Yutao

; Guruparan, Mahesan

; Gusso, Gustavo

; Gooden, Tiffany E

; Haniffa, Rashan

; Humphreys, Lindsey

; Jolly, Kate

; Jowett, Sue

; Kumarendran, Balachandran

; Lancashire, Emma

; Lane, Deirdre A

; Li, Xuewen

; Lip (Co-PI), Gregory Y.H.

; Li, Yan-guang

; Lobban, Trudie

; Lotufo, Paulo A

; Manseki-Holland, Semira

; Moore, David J

; Nirantharakumar, Krishnarajah

; Olmos, Rodrigo D

; Paschoal, Elisabete

; Pirasanth, Paskaran

; Powsiga, Uruthirakumar

; Romagnolli, Carla

; Santos, Itamar S

; Shantsila, Alena

; Sheron, Vethanayagam Antony

; Shribavan, Kanesamoorthy

; Szmigin, Isabelle

; Subaschandren, Kumaran

; Surenthirakumaran, Rajendra

; Tai, Meihui

; Neil Thomas (Co-PI), G

; Varella, Ana C

; Wang, Hao

; Wang, Jingya

; Zhang, Hui

; Zhong, Jiaoyue

Arquivos Brasileiros de Cardiologia

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2022, N. ahead

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Fundamento A associação entre o status de saúde cardiovascular ideal ( ideal cardiovascular health ( ICVH) e diagnóstico de fibrilação ou flutter atrial (FFA) foi menos estudado em comparação a outras doenças cardiovasculares. Objetivos Analisar a associação entre o diagnóstico de FFA e métricas e escores de ICVH no Estudo Longitudinal de Saúde do Adulto (ELSA-Brasil). Métodos Este estudo analisou dados de 13141 participantes com dados completos. Os traçados eletrocardiográficos foram codificados de acordo com o Sistema de Minnesota, em um centro de leitura centralizado. As métricas do ICVH (dieta, atividade física, índice de massa corporal, tabagismo, glicemia de jeju, e colesterol total) e escores do ICVH foram calculados conforme proposto pela American Heart Association . Modelos de regressão logística bruta e ajustada foram construídos para analisar associações de métricas e escores do ICVH com diagnóstico de FFA. O nível de significância foi estabelecido em 0,05. Resultados A idade mediana da amostra foi de 55 anos, e 54,4% eram mulheres. Nos modelos ajustados, os escores de ICVH não apresentaram associação significativa com diagnóstico de FFA prevalente [odds ratio (OR):0,96; intervalo de confiança de 95% (IC95%):0,80-1,16; p=0,70). Perfis de pressão arterial ideal (OR:0,33; IC95%:0,1-0,74; p=0,007) e colesterol total ideal (OR:1,88; IC95%:1,19-2,98; p=0,007) foram significativamente associados com o diagnóstico de FFA. Conclusões Não foram identificadas associações significativas entre escores de ICVH global e diagnóstico de FFA após ajuste multivariado em nossas análises, devido, ao menos em parte, às associações antagônicas da FFA com métricas de pressão arterial e de colesterol total do ICVH. Nossos resultados sugerem que estimar a prevenção da FFA por meio de escore de ICVH global pode não ser adequado, e as métricas do ICVH devem ser consideradas separadamente.

Abstract Background The association between ideal cardiovascular health (ICVH) status and atrial fibrillation or flutter (AFF) diagnosis has been less studied compared to other cardiovascular diseases. Objective To analyze the association between AFF diagnosis and ICVH metrics and scores in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Methods This study analyzed data from 13,141 participants with complete data. Electrocardiographic tracings were coded according to the Minnesota Coding System, in a centralized reading center. ICVH metrics (diet, physical activity, body mass index, smoking, blood pressure, fasting plasma glucose, and total cholesterol) and scores were calculated as proposed by the American Heart Association. Crude and adjusted binary logistic regression models were built to analyze the association of ICVH metrics and scores with AFF diagnosis. Significance level was set at 0.05. Results The sample had a median age of 55 years and 54.4% were women. In adjusted models, ICVH scores were not significantly associated with prevalent AFF diagnosis (odds ratio [OR]:0.96; 95% confidence interval [95% CI]:0.80-1.16; p=0.70). Ideal blood pressure (OR:0.33; 95% CI:0.15–0.74; p=0.007) and total cholesterol (OR:1.88; 95% CI:1.19–2.98; p=0.007) profiles were significantly associated with AFF diagnosis. Conclusions No significant associations were identified between global ICVH scores and AFF diagnosis after multivariable adjustment in our analyses, at least partially due to the antagonistic associations of AFF with blood pressure and total cholesterol ICVH metrics. Our results suggest that estimating the prevention of AFF burden using global ICVH scores may not be adequate, and ICVH metrics should be considered in separate.

https://doi.org/10.36660/abc.20210970

Growth and gas exchange of corn under salt stress and nitrogen doses
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Sousa, Henderson C.

; Sousa, Geocleber G. de

; Lessa, Carla I. N.

; Lima, Antonio F. da S.

; Ribeiro, Rute M. R.

; Rodrigues, Francisco H. da C.

Revista Brasileira de Engenharia Agrícola e Ambiental

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mar 2021, Volume 25 N. 3 Pages 174 - 181

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O excesso de sais pode afetar diversos processos nas culturas, e o nitrogênio (N) pode atenuar o efeito depressivo da salinidade. Objetivou-se avaliar a influência de doses de nitrogênio no crescimento e nas trocas gasosas da cultura do milho irrigado com água salina. O experimento foi conduzido no período de junho a setembro de 2019, na Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, CE. O delineamento experimental foi inteiramente casualizado, em arranjo fatorial 2 x 3 água de abastecimento de 0,3 dS m-1 e solução salina de 3,0 dS m-1, e três doses de nitrogênio, 0, 80 e 160 kg ha-1, correspondendo a 0, 50 e 100% da dose recomendada, respectivamente, com seis repetições. Aos 30 e 45 dias após a semeadura (DAS) foram avaliadas altura de planta, área foliar, número de folhas, fotossíntese, transpiração e a condutância estomática. O estresse salino afeta a altura de plantas, área foliar, fotossíntese, transpiração e a condutância aos 30 DAS. As doses de 80 e 160 kg ha-1 proporcionam maior desempenho em altura de plantas, área foliar, fotossíntese, transpiração e a condutância aos 30 DAS. O uso de água de baixa salinidade e as doses de 80 e 160 kg ha-1 foram mais eficientes quanto à altura de plantas, área foliar, fotossíntese, transpiração e a condutância aos 45 DAS. A dose de 160 kg ha-1 de N atenua os efeitos deletérios dos sais nas plantas de milho híbrido AG 1051 proporcionando maiores valores de fotossíntese, transpiração e condutância estomática aos 45 DAS, quando irrigadas com água de 3,0 dS m-1.

ABSTRACT The excess of salts can affect several processes in the crops, and nitrogen (N) can attenuate the depressive effect of salinity. The objective was to evaluate the influence of nitrogen doses on the growth and gas exchange of corn crop irrigated with saline water. The experiment was conducted from June to September 2019 at the University of International Integration of Afro-Brazilian Lusophony, Redenção, CE, Brazil. The experimental design was completely randomized, in a 2 x 3 factorial scheme (supply water of 0.3 dS m-1 and saline solution of 3.0 dS m-1) and three nitrogen doses, 0, 80, and 160 kg ha-1, corresponding to 0, 50, and 100% of the recommended dose respectively, with six repetitions. At 30 and 45 days after sowing (DAS), plant height, leaf area, number of leaves, photosynthesis, transpiration, and stomatal conductance were evaluated. Saline stress affects plant height, leaf area, photosynthesis, transpiration, and conductance at 30 DAS. The doses of 80 and 160 kg ha-1 provide greater performance in plant height, leaf area, photosynthesis, transpiration, and conductance at 30 DAS. The use of low salinity water and doses of 80 and 160 kg ha-1 were more efficient in terms of plant height, leaf area, photosynthesis, transpiration, and conductance at 45 DAS. The dose of 160 kg ha-1 of N attenuates the harmful effects of salts in AG 1051 hybrid maize plants, providing higher values of photosynthesis, transpiration, and stomatal conductance at 45 DAS when irrigated with water of 3.0 dS m-1.

https://doi.org/10.1590/1807-1929/agriambi.v25n3p174-181 307 downloads

The effect of incubation on business performance: A comparative study in the Centro region of Portugal
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Almeida, Rita Isabel da Silva

; Pinto, António Pedro Soares

; Henriques, Carla M. Ribeiro

Revista Brasileira de Gestão de Negócios

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mar 2021, Volume 23 N. 1 Pages 127 - 140

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo: O papel das incubadoras no desempenho das empresas é um tema que vem sendo discutido na literatura. Elas ajudam a criar as condições necessárias ao desenvolvimento do empreendedorismo e da inovação empresarial, mas faltam estudos sobre sua real contribuição, especialmente em contextos como o de Portugal. As incubadoras de empresas têm sido fortes impulsionadoras do empreendedorismo e da inovação. O objetivo principal deste estudo é avaliar se a incubação de empresas oferece benefícios às empresas incubadas em relação às não incubadas, principalmente em termos de desempenho. Metodologia: Os dados foram coletados de empresas incubadas e não incubadas da região Centro de Portugal. Esses dois grupos de empresas (incubadas e não incubadas) foram inicialmente comparados através do teste t e do teste de Mann-Whitney. Em seguida, por meio de modelos de regressão linear, estimou-se o impacto da incubação nas variáveis de desempenho, depois de ajustar ao efeito das variáveis de controle, quando significativas. Resultados: Os resultados sugerem que, nos primeiros anos de vida, as empresas incubadas apresentam um nível de desempenho superior às não incubadas, efeito que diminui à medida que as empresas amadurecem. Contribuições: O estudo contribui para aprofundar a compreensão do papel que as incubadoras de empresas desempenham, fornecendo mais evidências de que, nos estágios iniciais, as empresas incubadas apresentam desempenho superior às não incubadas.

Abstract Purpose: The role that incubators play in business performance is a topic that has been discussed in the literature. They help to create the necessary conditions for the development of entrepreneurship and business innovation, but studies on their real contribution are lacking, especially in contexts such as the Portuguese one. Business incubators have been strong drivers of entrepreneurship and innovation. The main objective of this study is to assess whether business incubation offers benefits to incubated companies compared to non-incubated ones, particularly in terms of performance. Design/methodology/approach Data were collected from incubated and non-incubated companies in the central region of Portugal. These two groups of companies (incubated and non-incubated) were initially compared using the t-test and the Mann-Whitney test. Then, using linear regression models, the impact of incubation on performance variables was estimated, adjusting for the effect of control variables, when significant. Findings The results suggest that in the first years of life, incubated companies present a higher level of performance than non-incubated ones, an effect that decreases as companies become more mature. Originality/value: The study contributes to deepening the understanding of the role that business incubators play, providing further evidence that in their early life incubated companies outperform non-incubated ones.

https://doi.org/10.7819/rbgn.v23i1.4089 239 downloads

10.

Brazilian Green Propolis: Chemical Composition of Essential Oil and Their In Vitro Antioxidant, Antibacterial and Antiproliferative Activities
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Quintino, Ricardo Lanzellotti

; Reis, Ana Carolina

; Fernandes, Cassia Cristina

; Martins, Carlos Henrique Gomes

; Colli, Ana Carla

; Crotti, Antônio Eduardo Miller

; Squarisi, Iara Silva

; Ribeiro, Arthur Barcelos

; Tavares, Denise Crispim

; Miranda, Mayker Lazaro Dantas

Brazilian Archives of Biology and Technology

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2020, Volume 63 elocation e20190408

Abstract: En | Text: En | PDF: En

Abstract Propolis is a resinous substance collected and processed by Apis mellifera from parts of plants, buds and exudates. In Minas Gerais (MG) state, Brazil, green propolis is produced from the collection of resinous substance found in shoot apices of Baccharis dracunculifolia. This paper aims to investigate the chemical composition and in vitro antioxidant, anti-Helicobacter pylori, antimycobacterial and antiproliferative activities of essential oil (EO) from Brazilian green propolis (BGP-EO). The oil showed high antibacterial activity against H. pylori (MIC = 6.25 µg/mL), Mycobacterium avium (MIC = 62.5 µg/mL) and M. tuberculosis (MIC = 64 µg/mL). Its antioxidant activity was evaluated in vitro by both DPPH (IC50 = 23.48 µg/mL) and ABTS (IC50 = 32.18 µg/mL) methods. The antiproliferative activity in normal (GM07492A, lung fibroblasts) and tumor cell lines (MCF-7, HeLa and M059J) was analyzed by the XTT assay. BGP-EO showed inhibition of normal cell growth at 68.93 ± 2.56 µg/mL. Antiproliferative activity was observed against human tumor cell lines, whose IC50 values were 56.17, 66.43 and -65.83 µg/mL for MCF-7, HeLa and M059J cells, respectively. Its major constituents, which were determined by GC-FID and GC-MS, were carvacrol (20.7 %), acetophenone (13.5 %), spathulenol (11.0 %), (E)-nerolidol (9.7 %) and β-caryophyllene (6.2 %). These results showed the effectiveness of BGP-EO as a natural product which has promising biological activities.

https://doi.org/10.1590/1678-4324-2020190408 1697 downloads

11.

Caregrief scale confirmatory factor analysis in a sample of portuguese caregivers
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Vasconcelos-Raposo, José

; Teixeira, Carla M.

; Ribeiro, Ana

; Silva, Ana

; Santos, Beatriz

PsychTech & Health Journal

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dec 2019, Volume 3 N. 1 Pages 30 - 42

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Há muita informação sobre o sofrimento dos cuidadores informais e formais. No entanto, em Portugal faltam escalas que avaliem esta dimensão do comportamento humano. O objectivo deste estudo foi validar e adaptar a Caregiver Grief Scale (CGS), desenvolvida por Franziska Meichsner, Denise Schinköthe e Gabriele Wilz (2016) para a população alemã, a fim de avaliar o grau de sofrimento dos cuidadores. Para analisar as propriedades psicométricas do instrumento, recorremos à análise factorial confirmatória com uma amostra de 150 cuidadores (formais e informais). Os resultados não confirmaram a proposta de um modelo de quatro factores (dor emocional, perda relacional, perda absoluta e aceitação de perda), tal como proposto na amostra alemã. Posteriormente, utilizou-se a análise factorial exploratória que sugeriu um modelo unifactorial, cuja análise factorial confirmatória mostrou-se mais adequada para a amostra do estudo, com valores de: χ² / df = 2.023; CFI = .970; GIF = .923; RMSEA = .083; AIC = 110.724. Além disso, a análise de consistência interna indicou um alfa de Cronbach de .936 e a Fiabilidade Compósita foi .934. Concluiu-se que a estrutura multifactorial sugerida para cuidadores alemães não foi replicada e que para a população portuguesa o sofrimento se apresenta como um constructo unifactorial. Recomenda-se que futuros estudos, em Portugal, sejam realizados com amostra diferenciadas de cuidadores

ABSTRACT There is a great deal of information about caregivers’ suffering, however, there is a lack of scales in Portugal that evaluate this dimension of human behavior. The purpose of this study was to validate and adapt the Caregiver Grief Scale (CGS), developed by Franziska Meichsner, Denise Schinköthe and Gabriele Wilz (2016) for the German population, in order to assess the degree of suffering of caregivers. In order to analyze the psychometric properties of the instrument, we used confirmatory factorial analysis with a sample of 150 caregivers (formal and informal). The results did not confirm the proposal of a 4-factor model (emotional pain, relational loss, absolute loss, and loss acceptance), as proposed by the German study. Subsequently, we used the exploratory factorial analysis that suggested a unifactorial model, which confirmatory factorial analysis showed was more adequate for the study sample, with values of: χ² / df = 2.023; CFI = .970; GIF = .923; RMSEA = .083; AIC = 110,724. In addition, the internal consistency analysis indicated a Cronbach alpha of .936 and the Composite Reliability was .934. It was concluded that the multifactorial structure suggested for German caregivers was not replicated and that for the Portuguese population suffering is a unifactorial construct. It is recommended that future studies, in Portugal, be carried out with a differentiated sample of caregivers.

https://doi.org/10.26580/pthj.art19-2019 42 downloads

12.

Behavioral characterization and alternative control methods of the Bamboo borer [Dinoderus minutus Fabricius (Coleoptera: Bostrichidae)]
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da Silva, Rudieli M.

; Ribeiro, Nathalia P.

; Rodrigues, Carla da S.

; Pelvine, Raíra A.

; Rondon, Josimara N.

; Cereda, Marney P.

Idesia (Arica)

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jun 2019, Volume 37 N. 2 Pages 5 - 12

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Bamboo wood is a widely used raw material for the manufacture of special furniture and handicraft products, and can also be used in construction and architecture. In Brazil, the usage of this material has gained great space in the last decades. However, it can suffer from the attack of the bamboo borer [Dinoderus minutus Fabricius (Coleoptera: Bostrichidae)], causing damages and making the use of this wood unviable. Hence, the purpose of this work was to verify the behavior of D. minutus in culms of three bamboo species (Bambusa vulgaris, B. vulgaris var. vittata e B. multiplex) in two seasons of the year, one dry and one rainy season, in addition to testing alternative methods of insect control. In relation to the behavior, we verified the damages caused by the larvae from the oviposition of D. minutus in nodes and internodes of the bamboo species. While the alternative control methods were based on the spraying of the bacterium Proteus mirabilis, as an entomopathogenic agent, on B. vulgaris nodes, and the immersion treatment of nodes of same bamboo species in aqueous extract of Dracaena arborea leaves, and in vinasse. In relation to the insect behavior, there was a higher activity (consumption and reproduction) during the rainy season and a higher attack preference for the species B. vulgaris. Regarding the alternative control methods, it was verified a better performance of the treatments with P. mirabilis and D. arborea, since they provided a mortality index superior to 90% of the insects.

RESUMEN La madera de bambú es una materia prima ampliamente utilizada para la fabricación de muebles especiales y productos de arte sanía. Además es empleada en la construcción civil y arquitectura. En Brasil, el uso de este material ha aumentado en las últimas décadas. Sin embargo, puede sufrir el ataque del barrenador de bambú Dinoderus minutus Fabricius (Coleoptera: Bostrichidae), causando daños e inutilizando la madera. El objetivo de este trabajo fue caracterizar el comportamiento de D. minutus en cañas de tres especies de bambú (Bambusa vulgaris, B. vulgaris var. vittata y B. multiplex) en dos estaciones del año, una seca y otra lluviosa, además de probar métodos alternativos de control de insectos. En relación con el comportamiento, verificamos los daños causados por las larvas a partir de la oviposición de D. minutus en nudos y entrenudos de las especies de bambú. Los métodos de control alternativos se basaron en la fumigación de la bacteria Proteus mirabilis, como agente entomopatógeno; en los nudos de B. vulgaris y el tratamiento de inmersión de nudos de la misma especie de bambú en extracto acuoso de hojas de Dracaena arborea y en vinaza. En relación con el comportamiento de los insectos, hubo una mayor actividad (consumo y reproducción) durante la temporada de lluvias y una mayor preferencia de ataque para la especie B. vulgaris. Respecto a los métodos de control alternativos, se verificó un mejor desempeño de los tratamientos con P. mirabilis y D. arborea, ya que proporcionaron un índice de mortalidad superior al 90% de los insectos.

919 downloads

13.

Gold Supported on Strontium Surface-Enriched CoFe2O4 Nanoparticles: a Strategy for the Selective Oxidation of Benzyl Alcohol
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Pereira, Laise N. S.

; Ribeiro, Carlos E. S.

; Tofanello, Aryane

; Costa, Jean C. S.

; Moura, Carla V. R. de

; Garcia, Marco A. S.

; Moura, Edmilson M. de

Journal of the Brazilian Chemical Society

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jun 2019, Volume 30 N. 6 Pages 1317 - 1325

Abstract: En | Text: En | PDF: En

The synthesis of efficient and reusable gold-based catalysts for the selective oxidation of alcohols is a strategy for the development of green processes. Pre-formed nanoparticles syntheses are an easy way to produce controlled-nanosized gold materials; however, the selection of a support is not trivial. Herein, we proposed a CoFe2O4 support enriched with Sr(OH)2, which holds remarkable properties and is suitable for the synthesis of a stable gold-based catalyst for the oxidation of benzyl alcohol. We suggested that the interaction between the CoFe2O4 and the Sr(OH)2 is highly important for the performance of the catalyst. Under the base-free condition of 2.5 h, 100 ºC and 2 bar of O2, the catalyst reached 58% of conversion with 76% of selectivity to benzaldehyde. With K2CO3 addition, the conversion and selectivity to benzaldehyde increased to 87% and 88%, respectively. Any gold leaching was detected in 5 successive runs, attesting the noticeable stability that the catalyst presents. This work provides great potential for the selective oxidation of alcohols with high activity since the magnetic properties of the catalyst provide an easy route and allows the separation of the medium reaction. In addition, we are here proposing an important interaction between the Sr(OH)2 and the magnetic nanoparticles.

https://doi.org/10.21577/0103-5053.20190030 803 downloads

14.

Pathogenicity of Metarhizium anisopliae and Beauveria bassiana fungi to Tetranychus ludeni (Acari: Tetranychidae)
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Pereira, Samuel Luan

; Reis, Tatiane Carla

; de Oliveira, Ivani Teixeira

; Ferreira, Evander Alves

; de Castro e Castro, Bárbara Monteiro

; Soares, Marcus Alvarenga

; Vidal Ribeiro, Victor Hugo

Arquivos do Instituto Biológico

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2019, Volume 86 elocation e0272018

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O uso de acaricidas é o principal método de controle de Tetranychus ludeni (Acari: Tetranychidae) em cultivos hortícolas. Esse ácaro foi registrado causando danos em batata-doce (Ipomoea batatas (L.). A utilização de fungos patogênicos é uma alternativa ao controle químico. O objetivo foi avaliar a patogenicidade dos fungos Metarhizium anisopliae (Meetch) Sorok. e Beauveria bassiana (Bals.) Vuill. ao ácaro fitófago T. ludeni em batata-doce. Suspensões conidiais de M. anisopliae e B. bassiana, nas concentrações de 106 e 107 con.mL-1, foram aplicadas sobre folhas de batata-doce. Após 24 horas, cinco fêmeas recém-emergidas foram liberadas sobre as folhas. O bioensaio foi inteiramente randomizado, com arranjo fatorial 2 × 2 (duas espécies de fungos e duas concentrações) e controle (água destilada), com 10 replicações por tratamento. A avaliação consistiu na observação de três parâmetros biológicos do ácaro: mortalidade, oviposição e repelência, após 24, 48, 72 e 96 horas de contato com os fungos. Os isolados de M. anisopliae causam altas taxas de mortalidade de T. ludeni em laboratório. Beauveria bassiana tem potencial para suprimir futuras gerações do ácaro, reduzindo a taxa de oviposição. Comportamento de repelência nos ácaros não foi observado.

ABSTRACT The use of acaricides is the main control method for Tetranychus ludeni (Acari: Tetranychidae) in horticultural crops. This mite has been recorded causing damage to sweet potato (Ipomoea batatas L.). The use of pathogenic fungi is an alternative to chemical control. The objective was to evaluate the pathogenicity of the fungi Metarhizium anisopliae (Meetch) Sorok, and Beauveria bassiana (Bals.) Vuill. to phytophagous mite T. ludeni in sweet potato plants. Conidial suspensions of M. anisopliae and B. bassiana, at concentrations of 106 and 107 con.mL-1 were applied to sweet potato leaves. After 24 hours, five females of T. ludeni newly emerged were released on the leaves. The bioassay was arranged in a completely randomized design with factorial arrangement 2 × 2 (two species of fungi and two concentrations) plus the control (distilled water), with 10 repetitions per treatment. The evaluation consisted of observing of three biological parameters of the mite: mortality, oviposition, and repellency, after 24, 48, 72, and 96 hours of contact with the fungi. The isolates of M. anisopliae cause high mortality rates of T. ludeni in laboratory. Beauveria bassiana has the potential to suppress future generations of mite, reducing its oviposition rate. Repellency behavior was not observed.

https://doi.org/10.1590/1808-1657000272018 934 downloads

15.

Factors associated with ASDAS remission in a long-term study of ankylosing spondylitis patients under tumor necrosis factor inhibitors
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Shimabuco, Andrea Y.

; Gonçalves, Celio R.

; Moraes, Julio C. B.

; Waisberg, Mariana G.

; Ribeiro, Ana Cristina de M.

; Sampaio-Barros, Percival D.

; Goldenstein-Schainberg, Claudia

; Bonfa, Eloisa

; Saad, Carla G. S.

Advances in Rheumatology

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2018, Volume 58 elocation 40

Abstract: En | Text: En | PDF: En

Abstract Objective: To determine the clinical and demographic factors associated with disease remission and drug survival in patients with ankylosing spondylitis (AS) on TNF inhibitors. Methods: Data from a longitudinal electronic database of AS patients under anti-TNF therapy between June/2004 and August/2013. Demographic, clinical parameters, disease activity by ASDAS remission (< 1.3) and inactive/low (< 2.1) were analyzed to characterize reasons for drug survival and switching of anti-TNF. Results: Among 117 AS patients, 69 (59%) were prescribed only one anti-TNF, 48 (41%) switched to a second anti-TNF and 13 (11%) to a third anti-TNF. Considering ASDAS-CRP < 1.3, 31 (39%) patients were inactive at the end of the study. Non-switchers (P = 0.04), younger age (P = 0.004), non-smoking (P = 0.016), shorter disease duration (P = 0.047), more frequent use of SSZ (P = 0.037) and lower BASDAI (P = 0.027), BASMI (P = 0.034) and BASFI (P = 0.003) at baseline were associated with remission. In the multivariate analysis younger age (P = 0.016) and lower BASDAI (P = 0.032) remained as remission predictors. Conclusion: This study supports that ASDAS-CRP remission is an achievable goal not only for non-switchers but also for second anti-TNF, particularly in patients with younger age and lower BASDAI at baseline. Comedication and non-smoker status seems to have a beneficial effect in anti-TNF response in this population.

https://doi.org/10.1186/s42358-018-0040-x 648 downloads

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