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Abstract This study aimed to understand the subjective meanings attributed to home care by family caregivers of dependent older adults through a multicenter qualitative investigation that gathered 84 in-depth interviews with family caregivers from eight Brazilian locations. The hermeneutic-dialectic, theoretical, methodological framework was employed. The following categories emerged from the analysis: 1. Movements inhibiting emotions and feelings; 2. Presence of processes of symbiosis and emotional dependence in the older adult-relative relationship; 3. Contentment in caring for the dependent older adult; and 4. Giving up current and future life projects. The results reveal life experiences marked by symbiotic processes, emotional dependence, and psychic stress. Dependence causes suffering and feelings of despair, powerlessness, impatience, but also solidarity and empathy. Withdrawal from the job market, social depreciation of family caregiver’s activity, social isolation, neglected self-care, and family conflicts impact caregivers’ subjectivity. The elaboration of public policies must consider the social-affective life experiences of family caregivers of dependent older adults in order to include the care of those providing care.

Resumo O objetivo do presente estudo foi compreender os sentidos subjetivos atribuídos pelos cuidadores familiares de idosos dependentes do cuidado ofertado no domicílio, por meio de uma investigação qualitativa multicêntrica que reúne entrevistas em profundidade com 84 cuidadores familiares em oito localidades no Brasil. Utilizou-se o referencial teórico metodológico hermenêutico-dialético. Na leitura das entrevistas emergiram as seguintes categorias que serviram de base para as análises: 1. Movimentos de inibição de emoções e sentimentos; 2. Presença de processos de simbiose e de dependência emocional na relação entre idoso e familiar; 3. Contentamento em cuidar do idoso dependente; e 4. Renúncia a projetos de vida atuais e futuros. Os resultados apontam vivências marcadas por processos de simbiose, dependência emocional e estresse psíquico. A dependência gera sofrimento e sentimentos de desespero, impotência, impaciência, mas também de solidariedade e empatia. A renúncia ao trabalho profissional, a desvalorização social da atividade de cuidador familiar, o isolamento social, o cuidado “descuidado” de si e os conflitos familiares influenciam a subjetividade dos cuidadores. A formulação de políticas públicas precisa considerar as vivências socioafetivas dos cuidadores familiares de idosos dependentes, para nelas incluir o cuidado de quem cuida.

https://doi.org/10.1590/1413-81232020261.30402020

Vivências subjetivas de familiares que cuidam de idosos dependentes
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Gutierrez, Denise Machado Duran

; Sousa, Girliani Silva de

; Figueiredo, Ana Elisa Bastos

; Ribeiro, Maria de Nazaré de Souza

; Diniz, Cleisiane Xavier

; Nobre, Guiomar Alegria Souza Silva

Ciência & Saúde Coletiva

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2021, Volume 26 N. 1 Pages 47 - 56

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1413-81232020261.30402020

Multidimensional voice assessment: the immediate effects of Lax Vox® in singers with voice complaints
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Matta, Ruliano Santana da

; Santos, Marco Aurélio Rocha

; Plec, Elisa Meiti Ribeiro Lin

; Gama, Ana Cristina Côrtes

Revista CEFAC

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2021, Volume 23 N. 2 elocation e4520

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Purpose: to multidimensionally assess the effect of the Lax Vox® vocal technique on singers with voice complaints. Methods: a comparative intrasubject study that assessed 30 singers - 13 males and 17 females, aged 18 to 55 years - who self-reported voice complaints and had voice problems symptoms. The participants were submitted to voice assessment with perceptive-auditory, acoustic, aerodynamic, and electroglottographic voice analysis, as well as laryngeal assessment with high-speed videolaryngoscopy. The participants were assessed at two moments: 1) at the beginning of the data collection; and 2) five minutes after performing the Lax Vox® vocal technique. The groups were compared with appropriate statistical tests, with a 5% significance level. Results: in the acoustic analysis, there was an increase in the fundamental frequency for males, after using the Lax Vox® vocal technique. In the aerodynamic assessment, there was an increase in the airflow mean values during vocalization, as well as in aerodynamic power after using the Lax Vox® vocal technique in both groups. Conclusion: the Lax Vox® vocal technique, in the studied singers with a complaint of dysphonia, promoted an increase in the fundamental frequency, for males. In the aerodynamic parameters, in both sexes, it promoted an increase in the airflow and aerodynamic power.

RESUMO Objetivo: avaliar, de forma multidimensional, o efeito da técnica vocal Lax Vox® em cantores com queixas vocais. Métodos: trata-se de um estudo comparativo intrassujeitos. Foram avaliados 30 cantores, sendo 13 homens e 17 mulheres, com idade entre 18 e 55 anos, que apresentaram queixa vocal autorreferida e presença de sintomas vocais. Os participantes foram submetidos à avaliação vocal pela análise perceptivo-auditiva, acústica, aerodinâmica e eletroglotográfica da voz, bem como à avaliação laríngea com a videolaringoscopia de alta velocidade. Os participantes foram avaliados em dois momentos: 1) no início da coleta; e 2) após cinco minutos de realização da técnica vocal Lax Vox®. Para comparação dos grupos foram utilizados teste estatísticos pertinentes, com nível de significância de 5%. Resultados: na análise acústica houve aumento da frequência fundamental dos homens após o uso da técnica vocal Lax Vox®. Na avaliação aerodinâmica houve aumento dos valores da média do fluxo aéreo durante vocalização e da potência aerodinâmica após o uso da técnica vocal Lax Vox® em ambos os grupos. Conclusão: a técnica vocal do Lax Vox® nos cantores com queixa de disfonia estudados promoveu um aumento da frequência fundamental nos homens. Nos parâmetros aerodinâmicos em ambos os sexos provocou um aumento do fluxo aéreo e da potência aerodinâmica.

https://doi.org/10.1590/1982-0216/20212324520

Livros para infâncias diversas: onze casos de editoras independentes da Argentina e do Brasil
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Mihal, Ivana

; Szpilbarg, Daniela

; Ribeiro, Ana Elisa

Estudos de Literatura Brasileira Contemporânea

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2021, N. 62 elocation e624

Abstract: En Pt Es | Text: En Pt Es | PDF: En | PDF: Pt | PDF: Es

Abstract In this article, based on an expanded and tentative concept of bibliodiversity that considers the subjects of sexual diversity, gender and diverse children, we briefly analyze the projects of six Argentine and five Brazilian independent publishing houses. In the 2000s, these publishers edited books about diversity, especially about transgender children and gender questions, directed towards children. Based in a brief analysis of the sites and catalogues (still in development), we conclude that the independent publishing houses have accompanied important changes and social achievements related to diversity rights, as demonstrated by the books shown here as examples.

Resumo Neste artigo, com base em uma noção expandida, e aqui ensaiada, de bibliodiversidade, que considera o tema da diversidade sexual, de gênero e as infâncias diversas, analisamos brevemente os projetos de seis editoras independentes argentinas e cinco brasileiras. Estas casas editoriais publicaram obras sobre temáticas ligadas à diversidade, em especial infâncias trans e questões de gênero, dirigidas ao público infantil, decorridos os anos 2000. Com base na análise dos sites e de seus breves catálogos (que ainda se constroem), concluímos que as editoras independentes têm caminhado pari passu com mudanças e conquistas sociais importantes em relação ao direito à diversidade, como demonstram livros aqui tomados como exemplos.

Resumen En este artículo, con base en una noción expandida y ensayada aquí, de bibliodiversidad, que considera el tema de la diversidad sexual, de género y las infancias diversas, analizamos brevemente los proyectos de seis editoriales independientes argentinas y cinco brasileñas. Estas casas editoriales publicaron obras sobre temáticas ligadas a la diversidad, especialmente infancias trans y cuestiones de género, dirigidas al público infantil desde los años 2000. Con base en un análisis de sitios web y de sus catálogos - que están todavía en construcción - concluimos que las editoriales independientes han ido acompañando los cambios y conquistas sociales importantes en relación con el derecho a la diversidad, como demuestran los libros aquí tomados como ejemplos.

https://doi.org/10.1590/2316-4018624

Serological screening for Chagas disease in an endemic region of Northern Minas Gerais, Brazil: the SaMi-Trop project
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Cruz, Dardiane Santos

; Souza, Núbia Nunes de

; Rafael, Aline Ferreira

; Damasceno, Renata Fiuza

; Ribeiro, Antonio Luiz Pinho

; Oliveira, Léa Campos de

; Sabino, Ester Cerdeira

; Ghilardi, Fábio de Rose

; Cruz Neto, Ozorino Caldeira

; Ferreira, Ariela Mota

; Haikal, Desirée Sant’Ana

; Cardoso, Clareci Silva

; Oliveira, Claudia Di Lorenzo

; Bierrenbach, Ana Luiza

; Vieira, Thallyta Maria

Revista do Instituto de Medicina Tropical de São Paulo

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2021, Volume 63 elocation e67

Abstract: En | Text: En | PDF: En

ABSTRACT Chagas disease (CD) is still a neglected disease. Infected individuals are diagnosed late, being treated in worse clinical conditions. Thus, this study aimed to analyze the prevalence and the factors associated with new confirmed cases of CD identified by serological screening in an endemic region of Minas Gerais State, Brazil. This is an analytical cross-sectional study with data from a project of the Research Center in Tropical Medicine of Sao Paulo- Minas Gerais (SaMi-Trop) conducted in two municipalities. Data collection included a questionnaire with closed questions, a venous blood collection and an ELISA serological test for CD. A total of 2,038 individuals with no previous diagnosis of CD participated in the study. The result of the serological test for CD was adopted as the dependent variable. The independent variables addressed personal issues, health conditions and lifetime housing. A descriptive analysis of individual variables was performed. Subsequently, a bivariate analysis was performed using the Pearson’s chi-square test. Households sheltering individuals positive for CD were georeferenced, and the analysis of spatial distribution was performed using the quartic function to estimate the density of the nucleus. Among the participants, 188 (9.2 %) were positive for CD. The profile of participants with CD was associated with place of residence, age, relative/family member with CD and living conditions. It is noteworthy that there are still patients with CD who are unaware of their diagnosis in both, rural and urban areas.

https://doi.org/10.1590/s1678-9946202163067

Avaliação de impacto de políticas públicas: o estudo de caso do PIBIC/ICMBio no Brasil
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Tozato, Heloisa de Camargo

; Bezerra, Fernanda de Araújo

; Martins, Elizabeth Maria Maia de Albuquerque

; Bacellar, Ana Elisa de Faria

; Salzo, Ivan

; Jorge, Rodrigo Silva Pinto

; Ribeiro, Katia Torres

Avaliação: Revista da Avaliação da Educação Superior (Campinas)

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dec 2020, Volume 25 N. 3 Pages 676 - 700

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Evidence-based impact assessment has a great potential to support decision making, improve the public policy cycle and support its transparency. Given this perspective, the present research had the objective of develop it using, as a case study, the Institutional Program of Scientific Initiation Scholarships of the Chico Mendes Institute for Biodiversity Conservation (PIBIC/ICMBio), which completed 10 years of implementation in Brazil. The Program is the ICMBio's tool to clarify which knowledge or information is essential to boost intervention actions for the Brazilian biodiversity management. The evaluation was based on documentary research, participant observation, the formulation of the result chain, consultation with key actors and the development of thematic cartography. The results showed the achievement of the PIBIC/ICMBio objectives; its relevance to other attributions of ICMBio; its coherence with the main Brazilian policies of fomentation and execution programs of research, protection, preservation and conservation of the biodiversity and of environmental education; its efficiency in relation to the costs involved; its added value ; and its potential for the Brazilian know-how to support decision making on biodiversity management and conservation.

A avaliação de impacto baseada em evidências apresenta elevado potencial para subsidiar a tomada de decisão, melhorar a implementação das políticas públicas e respaldar a transparência da gestão. Diante desta perspectiva, a presente pesquisa teve como objetivo desenvolvê-la utilizando, como estudo de caso, o Programa Institucional de Bolsas de Iniciação Científica do Instituto Chico Mendes de Conservação da Biodiversidade (PIBIC/ICMBio), o qual completou 10 anos de implementação no país. O Programa constitui a ferramenta do Instituto para explicitar quais conhecimentos ou informações são imprescindíveis para impulsionar as ações de intervenção para a gestão e manejo da biodiversidade brasileira. A avaliação foi realizada a partir da pesquisa documental, da observação participante, da formulação da cadeia de resultados, da consulta a atores-chaves e do desenvolvimento da cartografia temática. Os resultados evidenciaram o alcance dos objetivos do PIBIC/ICMBio; sua relevância para as demais atribuições do próprio ICMBio; sua coerência com as principais políticas brasileiras de fomento e execução programas de pesquisa, proteção, preservação e conservação da biodiversidade e de educação ambiental; sua eficiência em relação aos custos envolvidos; seu valor agregado e sua potencialidade para o know-how de apoio a tomada de decisão sobre manejo e conservação da biodiversidade no Brasil.

https://doi.org/10.1590/s1414-40772020000300009

[SciELO Preprints] - SUBJECTIVE LIFE EXPERIENCES OF FAMILY CAREGIVERS OF DEPENDENT OLDER ADULTS
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Gutierrez, Denise Machado Duran Sousa, Girliani Silva de Figueiredo, Ana Elisa Bastos Ribeiro, Maria de Nazaré Souza Diniz, Cleisiane Xavier Nobre, Guiomar Alegria Souza Silva

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Publication date: 2020-10-13

Abstract: En Pt

This study aimed to understand the subjective meanings attributed to home care by family caregivers of dependent older adults through a multicenter qualitative investigation that gathered 84 in-depth interviews with family caregivers from eight Brazilian locations. The hermeneutic-dialectic, theoretical, methodological framework was employed. The following categories emerged from the analysis: 1. Movements inhibiting emotions and feelings; 2. Presence of processes of symbiosis and emotional dependence in the older adult-relative relationship; 3. Contentment in caring for the dependent older adult; and 4. Giving up current and future life projects. The results reveal life experiences marked by symbiotic processes, emotional dependence, and psychic stress. Dependence causes suffering and feelings of despair, powerlessness, impatience, but also solidarity and empathy. Withdrawal from the job market, social depreciation of family caregiver’s activity, social isolation, neglected self-care, and family conflicts impact caregivers’ subjectivity. The elaboration of public policies must consider the social-affective life experiences of family caregivers of dependent older adults in order to include the care of those providing care.

Objetivou-se compreender os sentidos subjetivos atribuídos pelos cuidadores familiares de idosos dependentes ao cuidado ofertado no domicílio, por meio de uma investigação qualitativa multicêntrica que reúne entrevistas em profundidade com 84 cuidadores familiares em oito localidades no Brasil. Utilizou-se o referencial teórico metodológico hermenêutico-dialético. Na leitura das entrevistas emergiram as seguintes categorias que serviram de base para as análises: 1. Movimentos de inibição de emoções e sentimentos; 2. Presença de processos de simbiose e de dependência emocional na relação idoso e familiar; 3. Contentamento em cuidar do idoso dependente e 4. Renúncia a projetos de vida atuais e futuros. Os resultados apontam vivências marcadas por processos de simbiose, dependência emocional e estresse psíquico. A dependência gera sofrimento e sentimentos de desespero, impotência, impaciência, mas também solidariedade e empatia. A renúncia ao trabalho profissional, a desvalorização social da atividade de cuidador familiar, o isolamento social, o cuidado “descuidado” de si e conflitos familiares influenciam a subjetividade dos cuidadores. A formulação de políticas públicas precisa considerar as vivências socioafetivas dos cuidadores familiares de idosos dependentes para nelas incluir o cuidado de quem cuida.

https://doi.org/10.1590/SciELOPreprints.1258

10.

"Faça o que eu digo, não o que eu faço?" Um Estudo sobre a Transmissão Valorativa entre Pais e Filhos
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SILVA SOARES, ANA KARLA

; MEDEIROS CAVALCANTI, THIAGO

; FREIRE, SANDRA ELISA DE ASSIS

; REZENDE, ALESSANDRO TEIXEIRA

; RIBEIRO, MARIA GABRIELA COSTA

; GOUVEIA, VALDINEY VELOSO

Revista Colombiana de Psicología

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jun 2020, Volume 29 N. 1 Pages 29 - 44

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen El propósito de este estudio es identificar en qué medida los valores de los padres/madres e hijos se relacionan, teniendo en cuenta el tipo de transmisión (directa o indirecta) y su poder predictivo. Participaron 204 pares de padres e hijos. La mayoría de los niños era del sexo femenino (54.7%), con edad promedio de 11 años (DE =1.01). En cuanto a los progenitores, la mayoría fueron madres (69.6%), con edad promedio de 38 años (DE=7.82). Los progenitores contestaron la Encuesta de los Valores Básicos correspondiente a su grupo de edad y a cuestiones sociodemográficas. Los resultados señalan que los valores de los hijos son influenciados con más fuerza por los valores percibidos en sus padres/madres, que por los valores que los padres/madres priorizan o desean transmitir a sus hijos, presentando indicadores significativos en las seis subfunciones valorativas. Se concluye que, mediante el proceso de socialización, el niño aprehende los valores transmitidos por sus padres/madres desde la observación de sus conductas; por lo tanto, se enfatiza que el propio comportamiento es la mejor forma de educar a los hijos.

Abstract The objective of the study is to identify to what extent the values of fathers/mothers and children are correlated, taking into account the type of transmission (direct or indirect) and its predictive potential. Participants were 204 pairs of parents and children. Most of the children were female (54.7%), and their average age was 11 (DE=1.01). In the case of the parents, the majority were mothers (69.6%), and their average age was 38 (DE =7.82). The fathers/mothers answered the Basic Values Survey corresponding to their age group and socio-demographic aspects. Results show that children's values are more heavily influenced by the values they perceive in their fathers/mothers, than by the values the fathers/ mothers give priority to and wish to convey to their children, according to significant indicators in the six valuation sub-functions. The study concludes that, through the socialization process, children apprehend the values transmitted by their fathers/mothers by observing their conduct. Therefore, we emphasize that parents' behavior is the best way to educate children.

Resumo Este estudo objetiva identificar em que medida os valores dos pais/das mães e filhos estão relacionados, considerando o tipo de transmissão (direta ou indireta) e seu poder preditivo. Participaram 204 pares de pais e filhos. Dentre as crianças, a maioria era do sexo feminino (54.7%), com idade média de 11 anos (DP=1.01). Quanto aos pais, a maioria formada por mães (69.6%), com idade média de 38 anos (DP=7.82). Os pais/as mães responderam ao Questionário dos Valores Básicos correspondente ao seu grupo etário e a questões sociodemográficas. Os resultados indicaram que os valores dos filhos são influenciados mais fortemente pelos valores percebidos em seus pais/suas mães do que pelos valores que os pais/as mães priorizam ou almejam para seus filhos, apresentando indicadores significativos nas seis subfunções valorativas. Conclui-se que, por meio do processo de socialização, a criança apreende os valores transmitidos por seus pais/suas mães a partir da observação de seus comportamentos, portanto enfatiza-se que o próprio comportamento é a melhor forma para educar os filhos.

https://doi.org/10.15446/rcp.v29n1.72405

11.

Coleções de livros de bolso no Brasil: notas sobre a Biblioteca Universal Popular
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Ribeiro, Ana Elisa

; Karam, Sérgio

Palabra clave

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apr 2020, Volume 9 N. 2 Pages 84 - 84

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumo Estão em foco, neste trabalho, ao menos dois elementos da edição importantes para a compreensão dos modos de produção e circulação dos livros no Brasil: as noções de “livro popular” e de “livro de bolso”, ambas estreitamente relacionadas entre si e também a estratégias de produção e venda, formatos, design e certos gêneros, aqui no sentido de gêneros literários ou gêneros “de livraria”. Trataremos aqui, para fins de discussão conceitual, mas também para uma análise de práticas editoriais, da Biblioteca Universal Popular, coleção de livros de bolso publicada no país entre 1963 e 1968 pela Editora Civilização Brasileira, uma das mais importantes casas editoriais em atividade naquele momento. Outro aspecto relevante do presente estudo é a recomposição do catálogo da referida coleção, que representa uma das muitas tentativas de editoras brasileiras de acercar-se ao mercado dos livros de bolso por meio da edição de livros de baixo custo e ampla circulação. A coleta e a organização das informações aqui elencadas deram-se com base em consultas a bibliotecas particulares e a diversos sites, especialmente o acervo on-line da Fundação Biblioteca Nacional e a Estante Virtual.

Abstract In this work, we have focused in at least two elements of edition that are crucial to the understanding of the modes of production and the circulation of books in Brazil. These are the notions of “popular book” and “pocket book”, both closely related to each other and also to production and sales strategies, book formats, book design and certain literary genres or “bookstore” genres. For the purposes of conceptual discussion and analysis of editorial practices, we will deal here, with the Biblioteca Universal Popular (Universal Popular Library). This is a collection of paperbacks published in Brazil between 1963 and 1968 by Editora Civilização Brasileira, one of the most important publishing houses in activity at that time. Another relevant aspect of the present study is the recomposition of the catalog of this collection, which represents one of the many attempts of Brazilian publishers to approach the pocket book market through the edition of low cost books with a wide circulation. The collection and organization of the information presented here are based on investigations in private libraries and in various websites, especially in the online collection of Fundação Biblioteca Nacional and in the website of Estante Virtual.

https://doi.org/10.24215/18539912e084

12.

Gonadosomatic index and weight/length relationship in females of three penaeoidean shrimps impacted by fisheries on the southeastern Brazilian coast
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Santos, Renan Augusto Pereira dos

; Silva, Alexandre Ribeiro da

; Moraes, Isabela Ribeiro Rocha de

; Antunes, Mariana

; Lopes, Ana Elisa Bielert

; Costa, Rogério Caetano da

; Castilho, Antonio Leão

Nauplius

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2020, Volume 28 elocation e2020045

Abstract: En | Text: En | PDF: En

Abstract The aim of this study was to evaluate the gonadosomatic index (GSI) and the weight/length ratio in important shrimp populations (Xiphopenaeus kroyeri (Heller, 1862), Litopenaeus schmitti (Burkenroad, 1936), Rimapenaeus constrictus (Stimpson, 1874)) from the northern littoral of São Paulo State. The samples were collected between 2015 and 2016, with 212 females that were identified, classified according to their gonadal development stage, and measured (CL). The gonads were macroscopically classified into immature, spent and developed, and were then removed. Both body (BodW) and gonad (GonW) dry weights were obtained in order to calculate the gonadosomatic index. Weight/length relationship and weight gain rate were assessed. The weight gain presented negative allometry, indicating that the animals grow faster than they gain weight. The weight/length relationship showed a pattern of continuous reproduction, as previously determined for tropical species. From the GSI values, it was possible to separate the three predetermined gonadal stages, proving that macroscopic evaluation is reliable for evaluating the reproductive period of these shrimps. These results can be used as a guide for fishery stocks management and monitoring.

https://doi.org/10.1590/2358-2936e2020045

13.

Dengue diagnostics: serious inaccuracies are likely to occur if pre-analytical conditions are not strictly followed
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Iani, Felipe Campos de Melo

; Caetano, Ana Carolina Barbosa

; Cocovich, Jéssica Caroline Wenceslau

; Amâncio, Frederico Figueiredo

; Pereira, Maira Alves

; Adelino, Talita Émile Ribeiro

; Caldas, Sérgio

; Silva, Marcos Vinícius Ferreira

; Pereira, Glauco de Carvalho

; Duarte, Myrian Morato

Memórias do Instituto Oswaldo Cruz

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2020, Volume 115 elocation e200287

Abstract: En | Text: En | PDF: En

BACKGROUND The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES To check if pre-analytical variables, such as storage time and temperature, have an impact on the accuracy of the main routine diagnostic tests for dengue. METHODS Virus isolation, reverse transcription real-time polymerase chain reaction (RT-PCR) and NS1 enzyme-linked immunosorbent assay (ELISA) were evaluated using 84 samples submitted to different pre-analytical conditions. FINDINGS Sensitivity and negative predictive value were directly affected by sample storage conditions. RT-PCR and virus isolation showed greater dependence on well-conserved samples for an accurate diagnosis. Interestingly, even storage at -30ºC for a relatively short time (15 days) was not adequate for accurate results using virus isolation and RT-PCR tests. On the other hand, NS1 ELISA showed no significant reduction in positivity for aliquots tested under the same conditions as in the previous tests. MAIN CONCLUSIONS Our results support the stability of the NS1 marker in ELISA diagnosis and indicate that the accuracy of routine tests such as virus isolation and RT-PCR is significantly affected by inadequate transport and storage conditions of serum samples.

https://doi.org/10.1590/0074-02760200287

14.

Caffeic acid and chlorogenic acid cytotoxicity, genotoxicity and impact on global DNA methylation in human leukemic cell lines
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Hernandes, Lívia Cristina

; Machado, Ana Rita Thomazela

; Tuttis, Katiuska

; Ribeiro, Diego Luís

; Aissa, Alexandre Ferro

; Dévoz, Paula Pícoli

; Antunes, Lusânia Maria Greggi

Genetics and Molecular Biology

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2020, Volume 43 N. 3 elocation e20190347

Abstract: En | Text: En | PDF: En

Abstract Dietary phenolic compounds such as caffeic and chlorogenic acid exert an antiproliferative effect and modulate the gene-specific DNA methylation status in human breast tumor cells, but it remains unclear whether they interfere with global DNA methylation in human leukemia cells. We examined whether caffeic and chlorogenic acid (1-250 µM) exert antitumor action in human promyelocytic leukemia cells (HL-60) and human acute T-cell leukemia cells (Jurkat). Caffeic and chlorogenic acid did not reduce cell viability in the two cell lines, as assessed using the neutral red uptake and MTT assays. These phenolic acids (1-100 μM) neither induced DNA damage (comet assay) nor increased the micronuclei frequency (micronucleus assay) in HL-60 and Jurkat cells, indicating that they were not genotoxic or mutagenic. Analysis of global DNA methylation levels using a 5-mC DNA ELISA kit revealed that chlorogenic acid at a non-cytotoxic concentration (100 μM) induced global DNA hypomethylation in Jurkat cells, but not in HL-60 cells, suggesting that it exerts a cell-specific effect. Caffeic acid did not change global DNA methylation. As other phenolic compounds, chlorogenic acid probably modulates DNA methylation by targeting DNA methyltransferases. The hypomethylating action of chlorogenic acid can be beneficial against hematological malignances whose pathogenic processes involve impairment of DNA methylation.

https://doi.org/10.1590/1678-4685-gmb-2019-0347

15.

Brazilian Nutritional Consensus in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease
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Pereira, Andréa Z

; Vigorito, Afonso Celso

; Almeida, Alessandro de Moura

; Candolo, Alexandre de Almeida

; Silva, Ana Carolina Leão

; Brandão-Anjos, Ana Elisa de Paula

; Sá, Bianca Laselva de

; Souza, Catarina Lôbo Santos de

; Castro Junior, Cláudio Galvão de

; Oliveira, José Salvador Rodrigues de

; Barban, Juliana Bernardo

; Mancilha, Elaine Maria Borges

; Todaro, Juliana

; Lopes, Lilian Pinheiro

; Macedo, Maria Cristina Martins de Almeida

; Rodrigues, Morgani

; Ribeiro, Paulo Cesar

; Silva, Roberto Luiz da

; Roberto, Telma Sigolo

; Rodrigues, Thays de Cássia Ruiz

; Colturato, Vergilio Antonio Rensi

; Paton, Eduardo José de Alencar

; Barros, George Maurício Navarro

; Almeida, Rosana Ducatti Souza

; Moreira, Maria Claudia Rodrigues

; Flowers, Mary Evelyn

Einstein (São Paulo)

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2020, Volume 18 elocation eAE4799

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT The Brazilian Consensus on Nutrition in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease was approved by Sociedade Brasileira de Transplante de Medula Óssea , with the participation of 26 Brazilian hematopoietic stem cell transplantation centers. It describes the main nutritional protocols in cases of Graft- versus -host disease, the main complication of hematopoietic stem cell transplantation.

RESUMO O Consenso Brasileiro de Nutrição no Transplante de Células Tronco Hematopoiéticas: doença do enxerto contra o hospedeiro foi aprovado pela Sociedade Brasileira de Transplante de Medula Óssea, com a participação de 26 centros brasileiros de transplante de células-tronco hematopoiéticas. O Consenso descreve as principais condutas nutricionais em casos de doença do enxerto contra o hospedeiro, a principal complicação do transplante de células-tronco hematopoiéticas.

https://doi.org/10.31744/einstein_journal/2020ae4799

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