ABSTRACT The objective of this study was to describe the constant drying flux stage, as well as obtaining the critical moisture content during the convective drying of jambu leaf paste crushed under different conditions and characterizing the physical properties of the material after drying. Drying was carried out in an air circulation oven at a speed of 1.0 m s-1 and temperatures of 60, 70 and 80 ºC and layer thicknesses of 0.005 and 0.01 m. To determine the constant rate stage, the mass fluxes and the drying rate were calculated. Segmented polynomials were used to estimate the critical moisture content. Under the analyzed conditions, a typical drying behavior was found, demonstrating the existence of a significant period of constant drying rate for the mass of ground jambu leaves. Critical moisture content as a function of thickness and temperature did not show a trend. The material showed favorable physical characteristics, such as high solubility and cohesiveness, free flow, and good wettability, characteristics that add value to the raw material and for the availability of a new product to the market in the condition of packaging.

RESUMO Neste estudo objetivou-se descrever o estágio de fluxo de secagem constante, bem como obter o teor de água crítico durante a secagem convectiva da pasta de folhas de jambu trituradas em diferentes condições e caracterizar as propriedades físicas do material após a secagem. A secagem foi realizada em estufa de circulação de ar com velocidade de 1,0 m s-1 nas temperaturas de 60, 70 e 80 ºC e espessuras da camada de 0,005 e 0,01 m. Para a determinação do estágio de taxa constante, foram calculados os fluxos de massa e a taxa de secagem. Os polinômios segmentados foram utilizados para estimar o teor de água crítico. Nas condições analisadas houve um comportamento típico de secagem, demonstrando a existência de um expressivo período de taxa constante de secagem para a pasta de folhas de jambu trituradas. Os teores de água críticos em função da espessura e temperatura não apresentaram uma tendência. O material apresentou características físicas favoráveis, como alta solubilidade e coesividade, escoamento livre e boa molhabilidade, características que agregam valor à matéria-prima e para a disponibilização de um novo produto ao mercado em condição de acondicionamento.

Abstract The behavior of pile foundations under axial loading is directly influenced by the effects that its installation process induces in the surrounding soil. Consequently, the consideration of these effects is essential for the correct numerical modeling of these geotechnical structures. In the present study, numerical simulations of driven cast-in-situ piles under axial loading have been carried out using finite element analysis. Three 3.5 m long piles with diameters ranging from 114.3 to 219.1 mm were analyzed. The pile installation effects have been considered indirectly by employing two distinct approaches, both based on the concepts of cylindrical cavity expansion. The behavior of the tropical soil profile is described with the Hardening Soil constitutive model. The load-displacement response and load distribution along the pile obtained with the numerical simulations have been analyzed and compared with in-situ load tests results. In the failure conditions, both approaches accurately predicted the bearing capacity of the piles, with an average error of only 2% compared to the measured values. The results in terms of load distribution over depth were also satisfactory. The difference between measured and numerical ultimate base resistance values ranged from 0% to 30%. The good agreement between the numerical and experimental results indicates that the proposed numerical approaches have been effective in simulating the piles installation process and reinforces the importance of considering the installation effects in the numerical modeling of these geotechnical structures. Both approaches can also be used to predict the bearing capacity of displacement piles.

ABSTRACT Modeling diameter distribution in natural forests is an important tool for understanding the native woody species dynamics, supporting decision-making for degraded lands management and restoration. Therefore, this work aimed to fit probabilistic density functions to evaluate the diameter structure of three tree species with economic interest, such as Campomansesia xanthocarpa Marl. Ex. O. Berg, Piptadenia gonoacantha (Mart.) J.F. Macbr. and Zeyheria tuberculosa (Vell.) Bureau ex Verl., in a Semideciduous Seasonal Forest fragment at São Paulo State, Brazil. The data came from 83 temporary plots of 10 m x 20 m systematically distributed along 164 ha. Log-normal, Gamma, and Weibull functions were fitted to the three species using the fitdistrplus-package in the R program. Kolmogorov-Smirnov's adherence test was used to evaluate the fits at a 5% probability level. The functions were selected employing Akaike's Information Criterion (AIC) and Schwarz's Bayesian Information Criterion (BIC), in addition to a graphical analysis of the fitted functions. The results indicated that the three species diameter structure is positively asymmetric, representing the exponential pattern, representing continuous natural regeneration. AIC and BIC statistics indicated the Log-normal function to describe the diameter distribution of C. xanthocarpa and Z. tuberculosa, while the Gamma function was the most appropriate for P. gonoacantha. For the three species, the graphical analysis showed the Gamma function results in the best fit without tendency for estimating frequency density per diameter class.

RESUMO A modelagem da distribuição diamétrica em florestas naturais é uma ferramenta importante para compreender a dinâmica de espécies nativas lenhosas, auxiliando na tomada de decisão para o manejo e a recuperação de áreas degradadas. Dessa forma, o objetivo deste trabalho foi ajustar funções de densidade probabilística para avaliar a estrutura diamétrica de três espécies arbóreas de interesse econômico, como Campomansesia xanthocarpa Marl. Ex. O. Berg, Piptadenia gonoacantha (Mart.) J.F. Macbr. e Zeyheria tuberculosa (Vell.) Bureau ex Verl., em um fragmento de Floresta Estacional Semidecidual no estado de São Paulo, Brasil. Os dados foram provenientes de 83 parcelas temporárias de 10 m x 20 m distribuídas sistematicamente ao longo de 164 ha. As funções Log-normal, Gamma e Weibull foram ajustadas para as três espécies por meio do pacote fitdistrplus no programa R. O teste de aderência de Kolmogorov-Smirnov foi aplicado a 5% de probabilidade para avaliar os ajustes. As funções foram .selecionadas por meio do Critério de Informação Akaike (AIC) e do Critério de Informação Bayesiano de Schwaarz (BIC), além da análise gráfica das funções ajustadas. Os resultados indicaram que a estrutura diamétrica das três espécies é assimétrica positiva, as quais apresentam o padrão exponencial, sugerindo uma regeneração natural contínua. As estatísticas de AIC e BIC indicaram a função Log-normal para descrever a distribuição diamétrica de C. xanthocarpa e Z. tuberculosa, enquanto a função Gamma foi a mais adequada para P. gonoacantha. Para as três espécies, a análise gráfica mostrou que a função Gamma resultou no melhor ajuste, não apresentando tendências nas estimativas da densidade de frequências por classe de diâmetro.

ABSTRACT. In soils with adequate levels of fertility, it is possible to manage phosphate fertilization aiming at the grain production system, instead of each isolated crop. The objective was to evaluate the effects of the management of phosphate fertilization, place and time, on the soil and leaf P content, and crop yield in grain production systems. An experiment was conducted at the farm level in the municipality of Nazareno, state of Minas Gerais, Brazil, for four years on soil with adequate level of fertility. The treatments consisted of the management of phosphate fertilization by broadcast or furrow and dose for each crop or for the production system (spring/summer and autumn/winter crops), being: Control = without phosphate fertilization; Conventional = phosphate fertilization in the furrow in each crop; BTP = phosphate fertilization of the grain production system to broadcast; TPS = phosphate fertilization of the grain production system in the furrow of the spring/summer crop; TPW = phosphate fertilization of the grain production system in the furrow of the autumn/winter crop. Soil P content was evaluated in the third year of implantation, and the crops yield every season crop (maize, soybean, common bean and wheat). The P content in the leaves of maize and soybeans were evaluated in the spring/summer crops. The application of the total dose of phosphate fertilization for both crops in the sowing furrow of the spring/summer or autumn/winter crops (TPS and TPW) promoted operational advantages and increased soil P content in the 0-0.20 m layer, without reducing the yield of the spring/summer crops. Leaf P content was not affected. The Conventional management (phosphate fertilization in the furrow in each crop) provide greater yield of common beans and wheat during autumn/winter crops, which are more subject to water restriction due to less rainfall, even without increasing the soil P content.

BACKGROUND There is interest in lingering non-specific symptoms after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, referred to as Long coronavirus disease 2019 (Long COVID-19). It remains unknown whether the risk of Long COVID-19 is associated with pre-existing comorbidities or initial COVID-19 severity, including infections due to new Omicron lineages which predominated in 2023. OBJECTIVES The aim of this case report was to characterize the clinical features of acute XBB.1.5 infection followed by Long COVID-19. METHODS We followed a 73-year old female resident of Rio de Janeiro with laboratory-confirmed SARS-CoV-2 during acute infection and subsequent months. The SARS-CoV-2 lineage was determined by genome sequencing. FINDINGS The participant denied comorbidities and had completed a two-dose vaccination schedule followed by two booster doses eight months prior to SARS-CoV-2 infection. Primary infection by viral lineage XBB.1.5. was clinically mild, but the participant subsequently reported persistent fatigue. MAIN CONCLUSIONS This case demonstrates that Long COVID-19 may develop even after mild disease due to SARS-CoV-2 in fully vaccinated and boosted individuals without comorbidities. Continued monitoring of new SARS-CoV-2 lineages and associated clinical outcomes is warranted. Measures to prevent infection should continue to be implemented including development of new vaccines and antivirals effective against novel variants.

Abstract Background Some studies have suggested the HLA-B27 gene may protect against some infections, as well as it could play a benefit role on the viral clearance, including hepatitis C and HIV. However, there is lack of SARS-CoV-2 pandemic data in spondyloarthritis (SpA) patients. Aim To evaluate the impact of HLA-B27 gene positivity on the susceptibility and severity of COVID-19 and disease activity in axial SpA patients. Methods The ReumaCoV-Brasil is a multicenter, observational, prospective cohort designed to monitor immunemediated rheumatic diseases patients during SARS-CoV-2 pandemic in Brazil. Axial SpA patients, according to the ASAS classification criteria (2009), and only those with known HLA-B27 status, were included in this ReumaCov-Brasil's subanalysis. After pairing them to sex and age, they were divided in two groups: with (cases) and without (control group) COVID-19 diagnosis. Other immunodeficiency diseases, past organ or bone marrow transplantation, neoplasms and current chemotherapy were excluded. Demographic data, managing of COVID-19 (diagnosis, treatment, and outcomes, including hospitalization, mechanical ventilation, and death), comorbidities, clinical details (disease activity and concomitant medication) were collected using the Research Electronic Data Capture (REDCap) database. Data are presented as descriptive analysis and multiple regression models, using SPSS program, version 20. P level was set as 5%. Results From May 24th, 2020 to Jan 24th, 2021, a total of 153 axial SpA patients were included, of whom 85 (55.5%) with COVID-19 and 68 (44.4%) without COVID-19. Most of them were men (N = 92; 60.1%) with mean age of 44.0 ± 11.1 years and long-term disease (11.7 ± 9.9 years). Regarding the HLA-B27 status, 112 (73.2%) patients tested positive. There were no significant statistical differences concerning social distancing, smoking, BMI (body mass index), waist circumference and comorbidities. Regarding biological DMARDs, 110 (71.8%) were on TNF inhibitors and 14 (9.15%) on IL-17 antagonists. Comparing those patients with and without COVID-19, the HLA-B27 positivity was not different between groups (n = 64, 75.3% vs. n = 48, 48%, respectively; p = 0.514). In addition, disease activity was similar before and after the infection. Interestingly, no new episodes of arthritis, enthesitis or extra-musculoskeletal manifestations were reported after the COVID-19. The mean time from the first symptoms to hospitalization was 7.1 ± 3.4 days, and although the number of hospitalization days was numerically higher in the B27 positive group, no statistically significant difference was observed (5.7 ± 4.11 for B27 negative patients and 13.5 ± 14.8 for B27 positive patients; p = 0.594). Only one HLA-B27 negative patient died. No significant difference was found regarding concomitant medications, including conventional or biologic DMARDs between the groups. Conclusions No significant difference of COVID-19 frequency rate was observed in patients with axial SpA regarding the HLA-B27 positivity, suggesting a lack of protective effect with SARS-CoV-2 infection. In addition, the disease activity was similar before and after the infection. Trial registration This study was approved by the Brazilian Committee of Ethics in Human Research (CONEP), CAAE 30186820.2.1001.8807, and was registered at the Brazilian Registry of Clinical Trials – REBEC, RBR-33YTQC. All patients read and signed the informed consent form before inclusion.

ABSTRACT Endive is a vegetable traditionally eaten as a raw or cooked salad. It is a source of important nutritional compounds and one of the procedures for its industrialization is drying, which increases its shelf life, preserves the nutrients and reduces losses due to microorganisms. This research evaluated the drying kinetic of endive leaves at different temperatures and adjusted the experimental data according to mathematical models. The experimental design was completely randomized in triplicate, with each sample unit being a perforated aluminum tray containing about 100 g fresh leaves. The endive leaves were dried in an oven at 50, 60, 70 and 80°C. The mathematical models were adjusted according to the experimental data; non-linear regression analysis was performed by Gauss-Newton and Quasi-Newton methods. In all conditions, the mathematical models that best fitted the drying kinetics of the endive leaves were Midilli, Logarithmic and Valcam. The Logarithmic model, under these drying conditions, can be accurately described as suitable for predicting and simulating the drying kinetic of endive leaves, as it showed the best results in the statistical parameters evaluated.

RESUMO Chicória é um vegetal tradicionalmente consumido como salada crua ou cozida. É uma fonte de importantes compostos nutricionais e um dos procedimentos para sua industrialização é a secagem, que aumenta a vida útil, preserva seus nutrientes e reduz as perdas por microrganismos. Esta pesquisa avaliou a cinética de secagem de folhas de chicória em diferentes temperaturas e ajustou os dados experimentais de acordo com modelos matemáticos. O delineamento experimental foi inteiramente casualizado, em triplicata, onde cada unidade amostral era uma bandeja de alumínio perfurada, contendo cerca de 100 g de folhas frescas. As folhas de chicória foram secas em estufa, a 50, 60, 70 e 80°C. Os modelos matemáticos foram ajustados de acordo com os dados experimentais e, a partir deles, a análise de regressão não-linear foi realizada pelos métodos de Gauss-Newton e Quasi-Newton. Em todas as condições, os modelos matemáticos que melhor se ajustaram à cinética de secagem das folhas de chicória foram o Midilli, o Logarítmico e o Valcam. O modelo logarítmico, nessas condições de secagem, pode ser descrito com precisão como adequado para prever e simular a cinética de secagem das folhas de chicória, pois apresentou melhores resultados nos parâmetros estatísticos avaliados.

RESUMO O objetivo foi avaliar a divergência genética entre acessos de espécies silvestres e híbridos interespecíficos F1 de tomateiro. Caracterizou-se seis acessos silvestres (Solanum pimpinellifolium ‘AF 26970’, S. galapagense ‘LA-1401’, S. peruvianum ‘AF 19684’, S. habrochaites var. hirsutum ‘PI-127826’, S. habrochaites var. glabratum ‘PI-134417’ e S. pennellii ‘LA-716’), a cultivar comercial Redenção e os respectivos híbridos interespecíficos F1: ‘Redenção’ x ‘AF 26970’, ‘Redenção’ x ‘LA-1401’, ‘Redenção’ x ‘AF 19684’, ‘Redenção’ x ‘PI-127826’, ‘Redenção’ x ‘PI-134417’ e ‘Redenção’ x ‘LA-716’. Foram avaliados 35 caracteres quantitativos, sendo 29 morfoagronômicos e seis fisiológicos. Realizou-se análises de componentes principais (ACP). Utilizou-se os métodos de otimização de Tocher, baseado na distância de Mahalanobis e a dispersão gráfica das variáveis canônicas, que seguiram a mesma tendência de agrupamento dos genótipos, formando três grupos distintos. As ACP permitiram observar algumas divergências genéticas não observadas pelos demais métodos. Ocorreu alta divergência entre os acessos das espécies e híbridos interespecíficos. A cultivar Redenção teve a maior dissimilaridade genética e os híbridos interespecíficos de S. lycopersicum com acessos silvestres, apresentaram maior similaridade morfoagronômica e fisiológica com os genitores silvestres.

ABSTRACT The objective of this study was to evaluate the genetic divergence among accessions of wild tomato species and interspecific F1 hybrids. Six wild accessions were characterized (Solanum pimpinellifolium ‘AF 26970’, S. galapagense ‘LA-1401’, S. peruvianum ‘AF 19684’, S. habrochaites var. hirsutum ‘PI-127826’, S. habrochaites var. glabratum ‘PI-134417’, and S. pennellii ‘LA-716’), the commercial cultivar Redenção, and the respective interspecific F1 hybrids: ‘Redenção’ x ‘AF 26970’, ‘Redenção’ x ‘LA-1401’, ‘Redenção’ x ‘AF 19684’, ‘Redenção’ x ‘PI-127826’, ‘Redenção’ x ‘PI-134417’, and ‘Redenção’ x ‘LA-716’. Thirty-five quantitative traits were evaluated, encompassing 29 morphoagronomic and six physiological characteristics. Principal component analysis (PCA) was performed. Tocher’s optimization method was used based on the Mahalanobis distance and the graphic dispersion of canonical variables, which followed the same trend of genotype clustering, forming three distinct groups. The PCA indicated some genetic divergences not shown by the other methods. A high divergence was observed among the species accessions and interspecific hybrids. The cultivar Redenção had the greatest genetic dissimilarity, and the interspecific hybrids of S. lycopersicum with wild accessions showed the greatest morphoagronomic and physiological similarity with the wild parents.

RESUMEN El objetivo de este trabajo fue comparar las variables hortícolas de la lechuga iceberg utilizando dos dosis de compost Bokashi, solo y en combinación, con el bioactivador Penergetic (Penergetic International AG®) frente a la fertilización mineral estándar (NPK) en tres ciclos de cultivo. Los experimentos se realizaron en un invernadero de plástico en la Universidade Estadual de Londrina, Brasil (548 msnm). Los tratamientos fueron: control negativo (agua solamente); control positivo (NPK, 4-14-8); Penergetic solo; Bokashi en dosis de 5 y 10 g/planta con o sin combinación con Penergetic. Las plantas de lechuga se cultivaron en macetas llenas de tierra. Se evaluaron biomasa comercial (BC), diámetro de cabeza (DC), altura de planta (AP) e índice de clorofila (IC). En los tres ciclos evaluados, los tratamientos Bokashi 10 g/planta, Bokashi 10 g/planta + Penergetic y NPK superaron al testigo. En general, las variables estudiadas (BC, DC, AP y CI) en el tratamiento de menor dosis de Bokashi también fueron más altas que el control pero menores que con la dosis más alta de Bokashi y NPK. Penergetic aumentó la BC en el tratamiento con dosis más bajas de Bokashi solo en el primer ciclo de producción, probablemente debido al bajo contenido de materia orgánica en el suelo. El tratamiento Bokashi 10 g/planta mejoró significativamente las variables hortícolas de la lechuga frente al tratamiento de control y fue similar a la fertilización química en dos de los tres ciclos de cultivo.

ABSTRACT The aim of the study was to compare horticultural variables of iceberg lettuce using two Bokashi compost doses, alone and in combination, with the bioactivator Penergetic (Penergetic International AG®) against standard mineral fertilization (NPK) in three crop cycles. Experiments were conducted in a plastic greenhouse at the Universidade Estadual de Londrina, Brazil (548 m a.s.l.). The treatments were: negative control (water only); positive control (NPK, 4-14-8); Penergetic alone; Bokashi doses 5 g and 10 g/plant with or without a combination with Penergetic. Lettuce plants were grown in pots filled with soil. Commercial biomass (CM), head diameter (HD), plant height (PH) and chlorophyll index (CI) were evaluated. In the three cycles tested, the Bokashi 10 g/plant, Bokashi 10 g/plant + Penergetic, and NPK treatments surpassed the control. The studied variables (CM, HD, PH and CI) in the lower Bokashi dose treatment were also higher than controls but lower than higher Bokashi doses and NPK. Penergetic increased the CM in the lower Bokashi dose treatment just in the first production cycle, probably due to the poor organic matter content in the soil. The treatment Bokashi 10 g/plant improved significantly the lettuce horticultural variables vs. control treatments and was similar to chemical fertilization in two of three crop cycles.

RESUMO A cebola para produzir bulbos ou sementes depende da interação do genótipo com o ambiente. Assim, programas de melhoramento genético devem realizar a seleção com base nas condições climáticas de cada região produtora. Neste estudo, objetivou-se avaliar 17 híbridos experimentais de cebola e dois genótipos comercias (‘Bella Dura’ e ‘Buccaneer’) com base em atributos agronômicos, físico-químicos dos bulbos e de resistência ao florescimento precoce em condições subtropicais. Foi realizada análise de variância conjunta e a divergência fenotípica estimada por meio de análise de componentes principais (PCA) e agrupamento hierárquico de Ward. Observou-se que a época de transplante afetou mais as características agronômicas do que as características físico-químicas, não afetando a porcentagem de florescimento precoce dos genótipos. O transplantio de primeira época propiciou maiores produtividades de bulbos, enquanto, o transplante na segunda época, reduziu o ciclo e a produtividade. Os híbridos H1, H17 e ‘Buccaneer’ tiveram taxa de florescimento inferior a 3%. Entre os híbridos experimentais, H2 apresentou produtividade superior às cultivares comercias e, H1, H12 e H14 foram equivalentes a estas. No entanto, o H12 apresentou maior índice de florescimento precoce (43%). Assim, dentre os genótipos experimentais avaliados sob condições subtropicais, H1, H2, e H14, demonstraram potencial de uso comercial ou mesmo como potenciais genitores no avanço do programa de melhoramento, pois se destacaram por apresentar bom rendimento produtivo, qualidade de bulbo e baixo percentual de florescimento precoce.

ABSTRACT In order to produce bulbs or seeds, onion plants depend on genotype X environment interaction. Thus, breeders shall select the genotypes based on the climatic conditions of each producing region. This study aimed to evaluate 17 experimental onion hybrids and two commercial genotypes (‘Bella Dura’ and ‘Buccaneer’), based on agronomic, physicochemical attributes of the bulbs and resistance to early flowering under subtropical conditions. Joint analysis of variance and phenotypic divergence estimated through principal component analysis (PCA) and Ward’s hierarchical clustering were performed. We observed that the time of transplantation affected the agronomic traits more than the physicochemical traits, not affecting the percentage of early flowering of the genotypes. The first transplant season provided higher bulb productivity, whereas the second season reduced the cycle and productivity. Hybrids H1, H17 and ‘Buccaneer’ showed flowering rate lower than 3%. Among the experimental hybrids, H2 showed higher productivity when comparing with commercial cultivars, and H1, H12 and H14 were equivalent to them. However, H12 showed the highest early flowering rate (43%). Thus, considering the experimental genotypes evaluated under subtropical conditions, H1, H2, and H14, showed potential for commercial use or as potential parents in advancing the breeding program, as they stood out for presenting good yield, bulb quality and low percentage of early flowering.

Abstract The forest component's growth and production are important to asses the technical feasibility of an integrated livestock production system (iLPF) with native species from the Amazon. The objective of this work was to analyze and select hypsometric and volumetric equations for Dipteryx odorata and analyze its silvicultural development in an iLPF system in the Lower Amazon. Data were collected in the municipality of Mojuí dos Campos, Pará, using dendrometric and qualitative variables from 240 cumaru trees in a 1ha plantation with 9 years of implantation, in a spacing of 5 m x 7 m. Six hypsometric models and nine volumetric models were adjusted. To select each equation, the following criteria were observed: adjusted coefficient of determination (R²aj) standard error of the estimate in percentage (Sxy%) mean percentage deviation (DMP), the significance of the parameters by the t-test, normality of the residuals by the Kolmogorov- Test Smirnov and graphical analysis of percentage residuals. The simple linear model was the most suitable for estimating the volume's total height and the Husch model.

Resumo O crescimento e a produção do componente florestal são importantes para a avaliação da viabilidade técnica em sistema de produção de pecuária integrada (iLPF) com espécies nativas da Amazônia. O objetivo do trabalho foi analisar e selecionar equações hipsométricas e volumétricas para Dipteryx odorata e analisar seu desenvolvimento silvicultural em sistema iLPF no Baixo Amazonas. Os dados foram coletados no município de Mojuí dos Campos, Pará, usando variáveis dendrométricas e qualitativas de 240 árvores de cumaru em um plantio de 1ha com 9 anos de implantação, em um espaçamento de 5 m x 7 m. Foram ajustados seis modelos hipsométricos e nove modelos volumétricos. Para selecionar cada equação foram observados os seguintes critérios: coeficiente de determinação ajustado (R²aj) erro padrão da estimativa em porcentagem (Sxy%) desvio médio percentual (DMP), significância dos parâmetros pelo Teste t, normalidade dos resíduos por meio do Teste Kolmogorov-Smirnov e análise gráfica dos resíduos percentuais. O modelo linear simples foi o mais adequado para estimar a altura total e o modelo de Husch para o volume.

RESUMO A produção de cogumelos vem crescendo no Brasil, devido à versatilidade culinária desse alimento e alto valor nutricional. Ainda são escassos estudos que associam os aspectos de sua qualidade com o perfil e preferência dos seus consumidores. Assim, nesse trabalho foi comparada a firmeza e composição bioquímica de Shimeji e Shitake, e verificado o perfil e a preferência dos consumidores para compra. Os cogumelos foram caraterizados pela firmeza, teores de proteínas, lipídios, compostos fenólicos totais, flavonoides totais e atividade antioxidade, além da realização de pesquisa de opinião com 308 consumidores de cogumelos. Os dois cogumelos contêm alto teor de proteínas e baixo de lipídios, além de concentrações expressivas de compostos bioativos e de atividade antioxidante, desejáveis aos consumidores exigentes em alimentos funcionais. O Shitake é aproximadamente 4 vezes mais firme quer o Shimeji e os maiores teores para todos os atributos bioquímicos analisados não diferiram entre os dois cogumelos, exceto para o teor de proteínas. A pesquisa de opinião indicou que mulheres jovens e adultas consomem mais cogumelos que os homens. A maioria deles adquire os cogumelos em supermercados, e são pessoas com escolaridade, majoritariamente, de nível superior. Não foi constatada significativa maior preferência dos consumidores por um dos dois cogumelos analisados, mas a maioria dos participantes relataram que na compra priorizam cogumelos com aroma e sabor característicos, textura macia e suculenta, e que permita rapidez no preparo de refeições.

ABSTRACT Mushroom production has been growing in Brazil due to the culinary versatility of this food and its high nutritional value. However, few studies associating the aspects of its quality with the profile and preference of its consumers can be found in literature. Thus, in this study, we aimed to evaluate the firmness and biochemical composition of Shimeji and Shitake, as well as, the consumers’ profile and preference for purchasing these mushrooms. The mushrooms were characterized by firmness, protein and lipid content, total phenolic compounds, total flavonoids and antioxidation activity. In addition we carried out a survey on opinion of 308 mushroom consumers. The two mushrooms contain high protein content and low lipid content, and also expressive concentrations of bioactive compounds and antioxidant activity, suitable for these demanding functional food consumers. Shitake is approximately 4 times firmer than Shimeji and the highest levels for all analyzed biochemical attributes did not differ between the two studied mushrooms, except for protein content. The opinion survey indicated that young and adult women consume more mushrooms than men. Most of them buy mushrooms in supermarkets, and they are mostly higher educated people. No significant higher consumer preference for one of the two mushrooms analyzed was verified; however, most participants reported that they prioritize mushrooms which have characteristic aroma and flavor, smooth and juicy texture, being a convenient ingredient for a quick-cooking meal.

The Brazilian National Health System (BR-NHS) is one of the largest public health systems in the world. In 2019 Brazil had 114,352 community pharmacies (76.8% private owned), that represent the first point of access to healthcare in Brazil due to their wide distribution. Unfortunately, from the government's point of view, the main expected activity of private and public community pharmacies is related to dispensing medicines and other health products. Public community pharmacies can be part of a healthcare center or be in a separate location, sometimes without the presence of a pharmacist. Pharmacists working in these separated locations do not have access to patients' medical records, and they have difficulty in accessing other members of the patient care team. Pharmacists working in public pharmacies located in healthcare centers may have access to patients' medical records, but pharmacy activities are frequently under other professional's supervision (e.g., nurses). Private pharmacies are usually open 24/7 with the presence of a pharmacist for 8 hours on business days. Private community pharmacies have a very limited integration in the BR-NHS and pharmacists are the third largest healthcare workforce in Brazil with more than 221,000 registered in the Brazilian Federal Pharmacist Association [CFF - Conselho Federal de Farmácia]. A University degree in pharmacy is the only requirement to entry into the profession, without any proficiency exam for maintenance or career progression. The Brazilian pharmacist's annual income is ranked as the 2nd better-paid profession with an annual average income of EUR 5,502.37 (in 2020). Description of clinical activities for pharmacies by the CFF increased in the recent years, however there is still a long way to effectively implement them into practice.

Resumo Este trabalho avaliou a regeneração natural em área plantada há 13 anos com diferentes proporções de leguminosas fixadoras de nitrogênio. O plantio foi realizado em 2001, em pastagem abandonada, em Valença, sul do estado do Rio de Janeiro, com seis espécies florestais arbóreas não nodulantes, consorciadas com sete espécies leguminosas arbóreas nodulantes, de forma a estabelecer quatro proporções de leguminosas (30, 50, 65 e 80%) na composição botânica inicial. O delineamento experimental foi o de blocos ao acaso, com três repetições em parcelas de 940 m2. Em 2014, foram avaliados indivíduos regenerantes (≥ 60 cm) dentro de cada parcela e as espécies foram identificadas e classificadas quanto ao hábito de crescimento, grupo ecológico e síndrome de dispersão. Quantificou-se densidade absoluta, frequências absoluta e relativa e os índices de diversidade de Shannon e de equabilidade de Pielou. Foram encontradas 37 espécies que não haviam sido plantadas inicialmente. Somente três, das 13 espécies plantadas estiveram presentes no banco de plântulas. A maioria das espécies regenerantes eram arbóreas, pioneiras, com hábito de dispersão por zoocoria. O plantio misto com leguminosas arbóreas nodulantes é eficaz na ativação do processo de sucessão ecológica, uma vez que garante um alto recrutamento de espécies nativas regenerantes, com destaque para a composição botânica com 50 e 65%.

Abstract This study aimed to evaluate the ecological succession under different proportions of nitrogen-fixing trees intercropped with non-nitrogen fixing trees species through the understory floristic survey. A randomized block design with four treatments and three replications was conducted for 13 years with 30, 50, 65, and 80% of nitrogen-fixing trees in the whole plants used per plot. Fixed subplots with 10 m x 10 m were installed at the experimental plots and evaluated all individuals with height ≥ 60 cm to carry out the floristic survey. The species were identified and classified according to growth habit, the successional class, and dispersion syndrome. The natural regeneration vertical structure was evaluated with the following indicators: absolute density, absolute frequency, and relative frequency, the horizontal structure with Shannon’s diversity index and Pielou’s equitability index. The survey founded 37 species that had not been planted initially. Only three of the 13 planted species were present in the seedling bank. It was identified a more significant presence of pioneer trees species with zoochoric dispersion in the four treatments understory, indicating that the species mixed allowed the activation of the natural succession process. Treatments with 50 and 65 % of nitrogen-fixing trees favoured the diversity of species.

RESUMO O quiabo é vegetal consumido em vários países e é importante fonte de nutrientes para a alimentação humana. Para promover a produção e o consumo de quiabo, é essencial o desenvolvimento de novos genótipos com altos níveis nutricionais, além de características agronômicas desejáveis. Assim, este estudo teve como objetivo avaliar nove genótipos de quiabo por características agronômicas e bioquímicas. Os acessos UEL2, UEL3, UEL5, UEL6, UEL7, UEL9, UEL10, UEL13 e UEL15 foram cultivados em condições de campo e avaliados em relação à produtividade, peso do fruto, diâmetro do fruto, número de frutos por planta e altura da planta. As análises bioquímicas incluíram composição mineral, teor de proteína bruta, teor de vitamina C, teor total de flavonoides e fenólicos e atividade antioxidante. O genótipo UEL7 destacou-se pelos altos níveis de características bioquímicas, como conteúdo de alguns minerais, vitamina C e atividade antioxidante. Por outro lado, o UEL3 apresentou baixo desempenho para características bioquímicas, mas destacou-se por características agronômicas, como produtividade e número de frutos por planta. Portanto, as raças UEL7 e UEL3 podem ser introduzidas em programas de melhoramento focados na alimentação humana e no desempenho agronômico, além de contribuir para a conservação de germoplasma de quiabo.

ABSTRACT Okra is consumed in several countries and is an important source of nutrients for human food. To promote okra production and consumption is essential the development of new cultivars with high nutritional levels, besides desirable agronomic traits. Thus, this study aimed to evaluate nine okra landraces by agronomic and biochemical traits. The accessions UEL2, UEL3, UEL5, UEL6, UEL7, UEL9, UEL10, UEL13 and UEL15 were grown in open field condition and evaluated in relation to fruit yield, fruit weight, fruit diameter, number of fruits per plant and plant height. The biochemical analysis included mineral composition, crude protein content, vitamin C content, total flavonoid and phenolic content and antioxidant activity. The UEL7 accession stood out for the high levels for biochemical traits such as content of some minerals, vitamin C and antioxidant activity. On the other hand, UEL3 accession had a low performance for biochemical traits, but stood out for agronomic traits such as fruit yield and number of fruits per plant. Hence, the UEL7 and UEL3 landraces may be introduced in breeding programs focused on human food and agronomic performance, beyond to contribute for okra germplasm conservation.