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ABSTRACT BACKGROUND: A bibliometric analysis covering only the production of original studies or considering world production until 2022 has yet to be conducted. The creation and advancement of vaccines have also influenced research priorities, demonstrating the need for a new approach to this subject. OBJECTIVES: To analyze worldwide scientific production related to coronavirus disease 2019 (COVID-19) and the aged and to describe what has already been produced. DESIGN AND SETTING: Bibliometric analysis with a quantitative approach. METHOD: The search terms “COVID-19,” “SARS-CoV-2,” “Aged,” and “Elderly” were used to retrieve articles from the Web of Science database. A total of 684 articles were included in the analysis. Data were imported into RStudio Desktop Software and linked to R Software. The Bibliometrix R package and VOSviewer software were used for analysis. RESULTS: Most articles were published in 2020. These were produced by 4,937 authors and published in 379 journals. The keyword most used by the authors was “COVID-19.” Publications from 77 countries were obtained. China had the highest article production ranking, and Spain collaborated the most. The articles addressed the implications of the pandemic on the aged, the relationship between vaccination in the aged, and the implications for the disease itself. CONCLUSION: Further research should be conducted, mainly concerning vaccines and vaccination of the aged, owing to the need for and importance of immunization in this risk group, including assessing the long-term effects of vaccines.

https://doi.org/10.1590/1516-3180.2022.0662.r1.190523

Effects of the application of sodium ascorbate after in-office bleaching on the penetration of hydrogen peroxide, color change, and microtensile bond strength
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Mena-Serrano, Alexandra

; Granda-Albuja, María G.

; Naranjo, Jenny

; Fierro, Eliana Aldás

; Favoreto, Michael Willian

; Loguercio, Alessandro D.

; Reis, Alessandra

Brazilian Dental Journal

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oct 2023, Volume 34 N. 5 Pages 87 - 94

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract This study aimed to evaluate the effects of the application of 10% sodium ascorbate (SA) after in-office bleaching on the penetration of hydrogen peroxide (HP) into the pulp chamber, color change, and microtensile bond strength (µTBS) to the resin-enamel interface. Thirty premolars and thirty molars were randomly divided into three groups (n = 20 each). One group was exposed to deionized water (negative control). The other two groups were bleached with 35% HP in a single session for 3x15 minutes for each application. However, in only one of them, SA was applied for 10 minutes after bleaching. After, the concentration (µg/mL) of HP in each pulp chamber was evaluated by UV-Vis spectrophotometry. Color changes (ΔEab, ΔE00, and ΔWID) were evaluated with a digital spectrophotometer before and in the first week after bleaching. After treatment, molars were restored and sectioned to obtain resin-enamel interface sticks for µTBS at a crosshead speed of 1 mm/min until failure. The HP concentration and µTBS data were analyzed using one-way ANOVA and Tukey tests, and color changes were analyzed by t-tests (α = 0.05). SA application significantly improved the µTBS values and reduced the HP concentrations within the pulp chambers (p < 0.0001). The application of SA significantly interfered with the color changes after bleaching when compared to the control group (p < 0.05). Application of 10% SA after in-office bleaching successfully reduced the penetration of HP into the pulp chamber; however, it decreased color change.

Resumo Este estudo teve como objetivo avaliar os efeitos da aplicação do ascorbato de sódio a 10% (AS) depois do clareamento em consultório na penetração do peróxido do hidrogênio (PH) na câmara pulpar, mudança de cor e resistência de união (RU) da interface resina-esmalte. Trinta pré-molares e trinta molares foram divididos aleatoriamente em três grupos (n = 20). Um grupo foi exposto em água deionizada (controle negativo). Os outros dois grupos foram clareados com 35% PH numa única sessão de 3x15 minutos para cada aplicação. Porém, só um grupo recebeu AS durante 10 minutos depois do clareamento. Depois, a concentração (µg/mL) do PH no interior de cada câmara pulpar foi avaliado com espectrofotometria UV-Vis. A mudança de cor (ΔEab, ΔE00 and ΔWID) foi avaliada como espectrofotômetro digital antes e depois de uma semana do clareamento. Após de cada tratamento, os molares foram restaurados e seccionados em espécimes com interface resina-esmalte para o teste de RU por microtração a uma velocidade de 1 mm/min até a fratura. Os dados da concentração de PH e RU foram analisados usando ANOVA de uma via e teste de Tukey, e a mudança de cor com o teste t (α = 0.05). A aplicação de AS melhorou significativamente a RU e reduziu a concentração de PH na câmara pulpar (p < 0.0001). A aplicação de AS interferiu significativamente na mudança de cor depois do clareamento comprado com o grupo controle (p < 0.05). A aplicação de SA a 10% depois do clareamento em consultório reduziu significativamente a penetração do PH na câmara pulpar e interferiu na mudança de cor.

https://doi.org/10.1590/0103-6440202305214

Physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic repair material
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Campi, Lívia Bueno

; Rodrigues, Elisandra Márcia

; Torres, Fernanda Ferrari Esteves

; Reis, José Maurício dos Santos Nunes

; Guerreiro-Tanomaru, Juliane Maria

; Tanomaru-Filho, Mário

Brazilian Dental Journal

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feb 2023, Volume 34 N. 1 Pages 29 - 38

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract The aim of this study was to evaluate the physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic material, Bio-C Repair (Angelus), in comparison with White MTA (Angelus) and Biodentine (Septodont). The physicochemical properties of setting time, radiopacity, pH, solubility, dimensional and volumetric changes were evaluated. Biocompatibility and bioactivity were assessed in Saos-2 osteoblast cell cultures by the MTT assay 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Neutral Red (NR), Alizarin Red (ARS), and cell migration tests. Statistical analysis was performed by ANOVA, Tukey or Bonferroni tests (α = 0.05). Bio-C Repair had the longest setting time (p < 0.05), but radiopacity and solubility were accordance with the ISO 6876/2012 standards, besides linear expansion. Bio-C Repair and MTA had similar volumetric change (p > 0.05); lower than Biodentine (p < 0.05). All the materials evaluated had an alkaline pH. Bio-C Repair was cytocompatible and promoted mineralized nodule deposition in 21 days and cell migration in 3 days. In conclusion, Bio-C Repair had adequate radiopacity above 3mm Al, solubility less than 3%, dimensional expansion, and low volumetric change. In addition, Bio-C Repair promoted an alkaline pH and presented bioactivity and biocompatibility similar to MTA and Biodentine, showing potential for use as a repair material.

Resumo O objetivo deste estudo foi avaliar as propriedades físico-químicas, citotoxicidade e bioatividade de um novo material biocerâmico pronto para uso, Bio-C Repair (Angelus), em comparação com MTA Branco (Angelus) e Biodentine (Septodont). Foram avaliadas as propriedades físico-químicas de tempo de presa, radiopacidade, pH, solubilidade, alterações dimensionais e volumétricas. A biocompatibilidade, bioatividade e capacidade de reparo foram avaliadas em culturas de células de osteoblastos Saos-2 pelo ensaio MTT brometo de 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazólio), vermelho neutro (NR), vermelho de alizarina ( ARS) e testes de migração celular. A análise estatística foi realizada pelos testes ANOVA, Tukey ou Bonferroni (α = 0,05). Bio-C Repair apresentou o maior tempo de presa (p < 0,05), mas radiopacidade e solubilidade de acordo com as normas ISO 6876/2012, além de expansão linear. Bio-C Repair e MTA tiveram variação volumétrica semelhante (p > 0,05), menor que Biodentine (p < 0,05). Todos os materiais avaliados apresentaram pH alcalino. Bio-C Repair foi citocompatível, além de promover deposição de nódulos mineralizados em 21 dias e migração celular em 3 dias. Em conclusão, o Bio-C Repair apresentou radiopacidade adequada acima de 3mmAl, solubilidade menor que 3%, expansão dimensional e baixa perda volumétrica.. Além disso, o Bio-C Repair promoveu um pH alcalino e apresentou bioatividade e biocompatibilidade semelhantes ao MTA e Biodentine, mostrando potencial para uso como material reparador

https://doi.org/10.1590/0103-6440202304974

Association between abdominal obesity, screen time and sleep in adolescents
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Oliveira, Isabela dos Reis de

; Maciel, Nathália Maximiano Soares

; Costa, Bianca Tomaz da

; Soares, Anne Danieli Nascimento

; Gomes, Júnia Maria Geraldo

Jornal de Pediatria

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jan 2023, Volume 99 N. 1 Pages 45 - 52

Abstract: En | Text: En | PDF: En

Abstract Objective: To assess the association between abdominal obesity, food intake, sleep deprivation, and screen time in adolescents. Methods: This cross-sectional study was conducted with 432 adolescents aged 14–19 years, from public and private schools. Anthropometric and body composition measures included body weight, waist circumference, body mass index for age, waist-to-hip ratio, waist-to-height ratio, and body fat percentage. Abdominal obesity was defined by age and sex specific cut-off points for waist circumference. Food intake, screen time, sexual maturation, and sleep duration were evaluated by self-administered questionnaires. Results: The prevalence of excess body weight and abdominal obesity was 16.7% and 27.5%, respectively. Students in the adult phase (post-puberty), with inadequate waist-to-height ratio, high body fat percentage, and screen time ≥ 3 h/day were, respectively, 2.5 (95% CI 1.40–4.46), 7.44 (95% CI 1.08-51.46), 2.79 (95% CI 1.04-7.50), and 1.43 (95% CI 1.24-3.89) more likely to have abdominal obesity. Low intake of unprocessed or minimally processed foods was associated with inadequate sleep duration, while high intake of ultra-processed foods was associated with high screen time. Conclusions: Abdominal obesity was associated with the adult phase, short sleep duration and high screen time. The degree of food processing was associated with screen time and sleep duration. Assessing the risk factors of abdominal obesity may be a useful strategy for preventing cardiovascular disease in adolescents.

https://doi.org/10.1016/j.jped.2022.02.007

Nutritional evaluation and palatability of pet biscuits for dogs
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Poppi, Amanda Camila de Oliveira

; Oliveira, Gislaine Gonçalves

; Casetta, Jaisa

; Goes, Elenice Souza dos Reis

; Gasparino, Eliane

; Marques, Diogo de Oliveira

; Nogueira, Carla Cristina Alves

; Sbaraini, Sabrina Campos

; Santos, Sabrina Martins dos

; Khatlab, Angelica

; Vasconcellos, Ricardo Souza

; Cavali, Jucilene

; Dantas Filho, Jerônimo Vieira

; Souza, Maria Luiza Rodrigues de

Brazilian Journal of Food Technology

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2023, Volume 26 elocation e2022132

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract The study's aim was to evaluate the microbiological, nutritional, textural quality and palatability of pet biscuits made with the inclusion of three flours. The treatments were chicken viscera flour (CVF), commercial Nile tilapia flour (FF) and mixed fish flours of several species (FVF). For the FVF, only the carcasses (backbones) of Nile tilapia, salmon, tuna and sardines were used, and they were washed, whereas FVF as well as the others were cooked for 60 minutes, then pressed, ground and dehydrated for 24 hours at 60° C. After this period, they were crushed and ground for inclusion in the biscuits. The CVF, FF and FVF flours showed 43.82%, 50.48% and 56.70% of crude protein, respectively. CVF had the highest lipid content (10.01%) and FVF had the highest ash content (23.94%) and FVF had the highest caloric value (338.63 kcal 100 g-1). The inclusion of flours in biscuits did not influence the protein and carbohydrate contents, whose average values were 18.48% and 50.23%. However, they influenced the moisture content, lipids, ash and caloric value. Biscuits with the inclusion of viscera (CVF) had the highest lipid content (5.91%) and those with fish had the highest ash content (5.66% and 5.81%) for FF and FVF. Biscuits with FVF had a lower caloric value (318.47 kcal 100 g-1). The microbiological analysis indicated that the biscuits were considered of good quality to feed dogs. Regarding texture and palatability, there was no significant difference between treatments. The inclusion of fish flour, both FF and FVF, is a viable alternative.

Resumo Objetivou-se avaliar a qualidade microbiológica e nutricional, a textura e a palatabilidade de biscoitos pet elaborados com a inclusão de três farinhas. Os tratamentos foram farinha de vísceras de frango (FVC), farinha de tilápia comercial (FF) e mix de farinhas de peixes de várias espécies (FVF). Para FVF, foram utilizadas apenas as carcaças (espinhaço) de tilápia, salmão, atum e sardinha, as quais foram lavadas. Em todos os tratamentos, as farinhas foram cozidas por 60 minutos, depois prensadas, moídas e desidratadas por 24 horas a 60 °C. Decorrido esse período, as farinhas foram trituradas e moídas para inclusão nos biscoitos. As farinhas FVC, FF e FVF apresentaram 43,82%, 50,48% e 56,70% de proteína bruta, respectivamente. A FVC apresentou maior teor lipídico (10,01%) e a FVF, maior teor de cinzas (23,94%) e o maior valor calórico (338,63 kcal 100 g-1). A inclusão das farinhas nos biscoitos não influenciou nos teores de proteína e carboidratos, cujos valores médios foram de 18,48% e 50,23%, respectivamente. Entretanto, influenciaram no teor de umidade, lipídios, cinzas e valor calórico. Os biscoitos com inclusão de vísceras (CVF) apresentaram os maiores teores de lipídeos (5,91%) e aqueles com inclusão pescado maiores teores de cinzas (5,66% e 5,81%) para FF e FVF. Os biscoitos com FVF apresentaram menor valor calórico (318,47 kcal 100 g-1). A análise microbiológica indicou que os biscoitos apresentaram qualidades apropriadas para serem fornecidos a cães. Em relação a textura e aceitabilidade, não houve diferença significativa entre os tratamentos. A inclusão de farinhas de peixe, tanto FF quanto FVF, é uma alternativa viável.

https://doi.org/10.1590/1981-6723.13222

Parathyroidectomy: still the best choice for the management of severe secondary hyperparathyroidism
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Ramos, Luiz Guilherme Fernandes

; Cortes, Daniela Del Pilar Via Reque

; Reis, Luciene Machado dos

; Montenegro, Fabio Luiz de Menezes

; Arap, Sérgio Samir

; Brescia, Marília D’Elboux Guimarães

; Custódio, Melani Ribeiro

; Jorgetti, Vanda

; Elias, Rosilene Motta

; Moysés, Rosa Maria Affonso

Brazilian Journal of Nephrology

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2023, N. ahead

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Introduction: Management of secondary hyperparathyroidism (SHPT) is a challenging endeavor with several factors contruibuting to treatment failure. Calcimimetic therapy has revolutionized the management of SHPT, leading to changes in indications and appropriate timing of parathyroidectomy (PTX) around the world. Methods: We compared response rates to clinical vs. surgical approaches to SHPT in patients on maintenance dialysis (CKD 5D) and in kidney transplant patients (Ktx). A retrospective analysis of the one-year follow-up findings was carried out. CKD 5D patients were divided into 3 groups according to treatment strategy: parathyroidectomy, clinical management without cinacalcet (named standard - STD) and with cinacalcet (STD + CIN). Ktx patients were divided into 3 groups: PTX, CIN (cinacalcet use), and observation (OBS). Results: In CKD 5D we found a significant parathormone (PTH) decrease in all groups. Despite all groups had a higher PTH at baseline, we identified a more pronounced reduction in the PTX group. Regarding severe SHPT, the difference among groups was evidently wider: 31%, 14% and 80% of STD, STD + CIN, and PTX groups reached adequate PTH levels, respectively (p<0.0001). Concerning the Ktx population, although the difference was not so impressive, a higher rate of success in the PTX group was also observed. Conclusion: PTX still seems to be the best treatment choice for SHPT, especially in patients with prolonged diseases in unresourceful scenarios.

Resumo Introdução: O manejo do hiperparat-ireoidismo secundário (HPTS) é uma tarefa desafiadora com diversos fatores que contribuem para o fracasso do tratamento. A terapia calcimimética revolucionou o manejo do HPTS, levando a alterações nas indicações e no momento apropriado da paratireoidectomia (PTX) em todo o mundo. Métodos: Comparamos taxas de resposta às abordagens clínica vs. cirúrgica do HPTS em pacientes em diálise de manutenção (DRC 5D) e pacientes transplantados renais (TxR). Foi realizada uma análise retrospectiva dos achados de um ano de acompanhamento. Pacientes com DRC 5D foram divididos em 3 grupos de acordo com a estratégia de tratamento: paratireoidectomia, manejo clínico sem cinacalcete (denominado padrão - P) e com cinacalcete (P + CIN). Os pacientes com TxR foram divididos em 3 grupos: PTX, CIN (uso de cinacalcete) e observação (OBS). Resultados: Na DRC 5D, encontramos uma redução significativa do paratormônio (PTH) em todos os grupos. Apesar de todos os grupos apresentarem um PTH mais elevado no início do estudo, identificamos uma redução mais acentuada no grupo PTX. Com relação ao HPTS grave, a diferença entre os grupos foi evidentemente maior: 31%, 14% e 80% dos grupos P, P + CIN e PTX atingiram níveis adequados de PTH, respectivamente (p< 0,0001). Com relação à população TxR, embora a diferença não tenha sido tão impressionante, também foi observada uma taxa maior de sucesso no grupo PTX. Conclusão: A PTX ainda parece ser a melhor escolha de tratamento para o HPTS, especialmente em pacientes com doenças prolongadas em cenários sem recursos.

https://doi.org/10.1590/2175-8239-jbn-2023-0024en

Telehealth and telemedicine in the management of adult patients after hospitalization for COPD exacerbation: a scoping review
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Rezende, Lilian Cristina

; Ribeiro, Edmar Geraldo

; Parreiras, Laura Carvalho

; Guimarães, Rayssa Assunção

; Reis, Gabriela Maciel dos

; Carajá, Adriana Fernandes

; Franco, Túlio Batista

; Mendes, Liliane Patrícia de Souza

; Augusto, Valéria Maria

; Silva, Kênia Lara

Jornal Brasileiro de Pneumologia

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2023, Volume 49 N. 3 elocation e20220067

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective: A substantial number of people with COPD suffer from exacerbations, which are defined as an acute worsening of respiratory symptoms. To minimize exacerbations, telehealth has emerged as an alternative to improve clinical management, access to health care, and support for self-management. Our objective was to map the evidence of telehealth/telemedicine for the monitoring of adult COPD patients after hospitalization due to an exacerbation. Methods: Bibliographic search was carried in PubMed, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Web of Science, Scopus, Biblioteca Virtual de Saúde/LILACS and Cochrane Library databases to identify articles describing telehealth and telemonitoring strategies in Portuguese, English, or Spanish published by December of 2021. Results: Thirty-nine articles, using the following concepts (number of articles), were included in this review: telehealth (21); telemonitoring (20); telemedicine (17); teleconsultation (5); teleassistance (4); telehomecare and telerehabilitation (3 each); telecommunication and mobile health (2 each); and e-health management, e-coach, telehome, telehealth care and televideo consultation (1 each). All these concepts describe strategies which use telephone and/or video calls for coaching, data monitoring, and health education leading to self-management or self-care, focusing on providing remote integrated home care with or without telemetry devices. Conclusions: This review demonstrated that telehealth/telemedicine in combination with telemonitoring can be an interesting strategy to benefit COPD patients after discharge from hospitalization for an exacerbation, by improving their quality of life and reducing re-hospitalizations, admissions to emergency services, hospital length of stay, and health care costs.

RESUMO Objetivo: Um número substancial de pessoas com DPOC sofre de exacerbações, definidas como uma piora aguda dos sintomas respiratórios. Para minimizar as exacerbações, a telessaúde surgiu como alternativa para melhorar o manejo clínico, o acesso aos cuidados de saúde e o apoio à autogestão. Nosso objetivo foi mapear as evidências de telessaúde/telemedicina para o monitoramento de pacientes adultos com DPOC após hospitalização por exacerbação. Métodos: Foi realizada uma pesquisa bibliográfica nos bancos de dados PubMed, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Web of Science, Scopus, Biblioteca Virtual de Saúde/LILACS e Cochrane Library para identificar artigos que descrevessem estratégias de telessaúde e telemonitoramento em português, inglês, ou espanhol, publicados até dezembro de 2021. Resultados: Trinta e nove artigos, utilizando os seguintes conceitos (número de artigos), foram incluídos nesta revisão: telessaúde (21); telemonitoramento (20); telemedicina (17); teleconsulta (5); teleassistência (4); telecuidado domiciliar e telerreabilitação (3 cada); telecomunicação e saúde móvel (2 cada); e gestão de e-saúde, e-coach, teledomicílio, cuidados de telessaúde e tele/videoconsulta (1 cada). Todos esses conceitos descrevem estratégias que utilizam chamadas telefônicas e/ou de vídeo para coaching, monitoramento de dados e educação em saúde levando à autogestão ou autocuidado, com foco na prestação de cuidados domiciliares remotos integrados, com ou sem dispositivos de telemetria. Conclusões: Esta revisão demonstrou que a telessaúde/telemedicina associada ao telemonitoramento pode ser uma estratégia interessante para beneficiar pacientes com DPOC após a alta hospitalar por exacerbação, por meio da melhora da qualidade de vida e da redução das re-hospitalizações, admissões em serviços de emergência, tempo de internação hospitalar e custos de cuidados de saúde.

https://doi.org/10.36416/1806-3756/e20220067

Delinting and neutralizers residue effect on stored cotton seeds physiological quality determined by phenotyping image analysis
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Lima, Juliana Maria Espíndola

; Carvalho, Everson Reis

; Moraes, Luiz Fernando de Souza

; Cossa, Nasma Henriqueta da Sorte

; Miquicene, Felisberto Venâncio Chicamasso

; Gradela, Yuri Fazion

Journal of Seed Science

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2023, Volume 45 elocation e202345014

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract: The cottonseed delinting removes lint from the seed coat to improve plantability and maintain the seed’s physiological quality, therefore, this work aimed to determine the effect of delinting and neutralizers’ residual on the physiological quality of stored cottonseed by using image analysis phenotyping. The experiment was a randomized factorial design of 3 x 4 x 2 and two periods of storage evaluation (0 and 180 days). The cotton seeds were delinted at three different times (2, 4, and 10 minutes), neutralized using four distinct neutralizers (Quicklime, Hydrated lime, Filler lime, and NaOH), and there were seeds with and without neutralizer residue. The evaluations carried out were: water content, seed residual lint imaging, germination, seedling emergence, and seedling length imaging. The neutralizers Quicklime, Hydrated lime, and Filler lime do not need removal from the cotton seed coat. In storage, only the neutralizer NaOH has a high latent effect in lowering cottonseed quality, and the other neutralizers have lower physiological quality when the residue is removed. The image analysis phenotyping is efficient in identifying seedlings’ vigor.

Resumo: O deslintamento das sementes de algodão remove o linter do tegumento para melhorar a plantabilidade e manter a qualidade fisiológica da semente, por isso, este trabalho teve como objetivo determinar o efeito do deslintamento e do resíduo de neutralizantes na qualidade fisiológica de sementes de algodão armazenado por meio de fenotipagem usando análise de imagens. O experimento foi um delineamento casualizado em fatorial 3 x 4 x 2 e dois períodos de avaliação de armazenamento (0 e 180 dias). As sementes de algodão foram deslintadas em três tempos diferentes (2, 4 e 10 minutos), neutralizadas com quatro neutralizantes (Cal virgem, Calcário hidratada, Calcário filler e NaOH), e utilizou-se sementes com e sem resíduo de neutralizante. As avaliações realizadas foram: teor de água, imagem de linter residual em sementes, germinação, emergência de plântulas e imagem de comprimento de plântulas. Os neutralizantes Cal virgem, Calcário hidratado e Calcário filler não precisam ser removidos do tegumento da semente de algodão. No armazenamento, apenas o neutralizante NaOH apresenta alto efeito latente na redução da qualidade da semente de algodão, e os demais neutralizantes apresentam inferior qualidade fisiológica quando o resíduo é removido. A fenotipagem por análise de imagem é eficiente na identificação do vigor das plântulas.

https://doi.org/10.1590/2317-1545v45267297

Development and clinical application of hydrogel formulations containing papain and urea for wound healing
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Santos, Thais Menezes dos

; Sousa, Danieli Silva Feijó de

; Di Piero, Karina Chamma

; Todeschini, Adriane Regina

; Dias, Wagner Barbosa

; Oliveira, Catarina Amorim

; Santos, Elisabete Pereira dos

; Monteiro, Mariana Sato de Souza Bustamante

; Gomes, Maria Kátia

; Moura, Cristiano dos Reis

; Teixeira, Pedro Antônio Castelo

; Ricci-Junior, Eduardo

; Freitas, Zaida Maria Faria de

Brazilian Journal of Pharmaceutical Sciences

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2023, Volume 59 elocation e201090

Abstract: En | Text: En | PDF: En

Abstract Hydrogels are used for wound treatment, as they may contain one or more active components and protect the wound bed. Papain is one of the active substances that have been used with this purpose, alongside urea. In this paper, carboxypolymethylene hydrogels containing papain (2% and 10% concentrations) and urea (5% concentration) were produced. Physical-chemical stability was performed at 0, 7, 15 and 30 days at 2-8ºC, 25ºC and 40ºC, as well as the rheological aspects and proteolytic activity of papain by gel electrophoresis. Clinical efficacy of the formulations in patients with lower limb ulcers was also evaluated in a prospective, single-center, randomized, double-blind and comparative clinical trial. The results showed 7-day stability for the formulations under 25ºC, in addition to approximately 100% and 15% of protein activity for 10% and 2% papain hydrogel, respectively. The rheological profile was non-Newtonian for the 10% papain hydrogel tested. There were no significant differences regarding the mean time for healing of the lesions, although 10% papain presented a better approach to be used in all types of tissue present in the wound bed.

https://doi.org/10.1590/s2175-97902023e201090

10.

Quality of corn seed industrial seed treatment (IST) and on-farm treatment (OFT) in Brazilian agribusiness
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Medeiros, Jhonata Cantuária

; Carvalho, Everson Reis

; Andrade, Dayliane Bernardes de

; Moraes, Luiz Fernando de Souza

; Lima, Juliana Maria Espíndola

; Massa, Mônica Amurim Freitas

Journal of Seed Science

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2023, Volume 45 elocation e202345017

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract: Seed treatment is important for protecting seed and seedling from pests and/or pathogens. Seed treatment can be performed generally in two ways: industrial seed treatment (IST), performed by seed companies, or on-farm treatment (OFT). These treatment technologies will affect the final quality of the process. Therefore, the objective of this study was to characterize the seed quality profile of ISTs and OFTs in Brazilian agribusiness in terms of functional quality and dose application effectiveness (DAE). Seed samples treated by both processes were collected in 6 producing states of Brazil, Mato Grosso, Goiás, Paraná, Minas Gerais, São Paulo, and Santa Catarina. To evaluate the functional quality of the treatments, the coating percentage was determined by high-resolution image analysis with GroundEye® equipment, and the insecticide active ingredient DAE amount was determined with high-performance liquid chromatography (HPLC). Image analysis proved to be a promising technique to evaluate seed coating, in addition, samples treated with “on farm” technology had greater variation and heterogeneity in coating and active ingredient dose, while samples treated industrially showed greater homogeneity, uniformity, effectiveness of applied products. There was a strong positive correlation between coating with phytosanitary products and DAE in treated corn seeds, important parameters in the evaluation of the quality of the treatment.

Resumo: O tratamento de semente é importante para a proteção de sementes e plântulas contra pragas e ou patógenos. Podendo ser realizado basicamente via tratamento de sementes industrial (TSI) ou na própria fazenda “on farm” (TOF). Essas tecnologias de tratamento vão afetar a qualidade final do processo. Assim, o objetivo no trabalho foi realizar a caracterização do perfil da qualidade do TSI e TOF no agronegócio brasileiro, quanto à qualidade funcional e eficácia de aplicação de dose (EAD). Sementes tratadas em ambos os processos foram coletadas em 6 estados produtores do Brasil e avaliadas quanto à porcentagem de recobrimento por meio da análise de imagens com o equipamento GroundEye® e EAD do ingrediente ativo inseticida por meio de Cromatografia Líquida de Alta Eficiência (CLAE). A análise de imagem mostrou-se uma técnica promissora para avaliar o recobrimento de sementes, além disso, as amostras tratadas com a tecnologia “on farm” apresentaram maior variação e heterogeneidade do revestimento e da dose do ingrediente ativo, enquanto as amostras tratadas industrialmente houve maior homogeneidade, uniformidade, assertividade dos produtos aplicados. Existe forte correlação positiva entre recobrimento por produto fitossanitário e EAD em sementes tratadas de milho, parâmetros importantes na avaliação da qualidade do tratamento.

https://doi.org/10.1590/2317-1545v45268856

11.

An alternative approach to conducting germination tests on chemically treated and untreated stored cotton seeds
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Cossa, Nasma Henriqueta Da Sorte

; Carvalho, Everson Reis

; Lima, Juliana Maria Espíndola

; Moraes, Luiz Fernando de Souza

; Miquicene, Felisberto Venâncio Chicamasso

; Zevo, Ivan Natividade Júlio

Journal of Seed Science

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2023, Volume 45 elocation e202345027

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract: The objective of this study was to assess the impact of incorporating vermiculite into germination tests for both chemically treated and untreated stored cotton seeds. To that end, two independent experiments were conducted using cotton seeds of the cultivar DP1746B2RF under chemical treatment with a mixture of fungicide, insecticides, and nematicide; and both experiments were set up in a completely randomized design with four replications. In Experiment 1, the objective was to determine the ideal water volume for moistening the vermiculite. Four water volumes were used: 1.5, 2.0, 2.5, and 3.0 times the weight of the dry vermiculite. Experiment 2 was conducted in a 2 × 2 × 2 triple factorial arrangement consisting of two substrates (germitest paper and germitest paper + vermiculite), two treatments (chemically treated seeds and untreated seeds), and two storage periods (0 and 150 days). First germination count and germination were evaluated in the two experiments. From the results found in Experiment 1, the water volume corresponding to 3.0 times the weight of the vermiculite provided the best conditions for germination. In Experiment 2, the most appropriate substrate for conducting the germination test of untreated and chemically treated cotton seeds is vermiculite, which led to rapid germination and uniform seedling development.

Resumo: Objetivou-se avaliar o efeito da inclusão da vermiculita no teste de germinação em sementes de algodão não tratadas e tratadas quimicamente. Para isso, foram conduzidos dois experimentos independentes, utilizando-se sementes de algodão da cultivar DP1746B2RF, submetidas ao tratamento químico com mistura de fungicida, inseticidas e nematicida, ambos, instalados em delineamento inteiramente casualizado com quatro repetições. No Experimento 1, objetivou-se determinar o volume de água ideal para o umedecimento da vermiculita. Foram utilizados quatro volumes de água: 1,5; 2,0; 2,5 e 3,0 vezes o peso da vermiculita seca. O Experimento 2 foi conduzido em esquema fatorial triplo 2 x 2 x 2 sendo: dois substratos (papel germitest e papel germitest + vermiculita), dois tratamentos (sementes tratadas quimicamente e sementes não tratadas) e dois períodos de armazenamento (0 e 150 dias). Avaliou-se nos dois experimentos, a primeira contagem de germinação e a germinação. Pelos resultados encontrados no Experimento 1, o volume de água correspondente a 3,0 vezes o peso da vermiculita proporcionou as melhores condições para germinação. No Experimento 2, o substrato mais apropriado para condução do teste de germinação de sementes de algodão não tratadas e tratadas quimicamente, é a vermiculita, tendo proporcionado rápida germinação e desenvolvimento uniforme das plântulas.

https://doi.org/10.1590/2317-1545v45266896

12.

Religiosity and Spirituality: The Relationship Between Psychosocial Factors and Cardiovascular Health
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Cruz, José Icaro Nunes

; Cardozo, Adelle Cristine Lima

; Melo, Enaldo Vieira de

; Lira, Juliana Maria Chianca

; Santos, Giulia Vieira

; Salazar, Gabriela de Oliveira

; Oliveira, Bruna

; Reis, Mariano César de Souza

; Lima, Diego Maldini Borba de

; Soares, Philipi Santos

; Sousa, Antônio Carlos Sobral

; Oliveira, Joselina Luzia Menezes

International Journal of Cardiovascular Sciences

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2023, Volume 36 elocation e20220176

Abstract: En | Text: En | PDF: En

Abstract Background: Religiosity and Spirituality (R/S), despite being different entities, are multidimensional constructs, whose influence on cardiovascular health has been increasingly studied in recent decades. Objectives: To discriminate patients into subgroups according to R/S levels, in order to compare them regarding the distribution of cardiovascular comorbidities and clinical events. Methods: This is an observational, cross-sectional, analytical study. Two R/S scales were applied to a sample of patients seen at cardiology outpatient clinics. A cluster analysis was used to discriminate individuals into subgroups regarding R/S levels, which were subsequently compared regarding the frequencies of clinical variables related to cardiovascular health. A significance level of 5% was set for the statistical tests. Results: The sample included 237 patients with a mean age of 60.8 years (±10.7), of which 132 were female (55.7%). Cluster analysis (C) distinguished two groups: C1, with lower levels of R/S, and C2, with higher levels of R/S (p<0.001). C2 had a lower frequency of alcohol consumption (29.5% vs. 76.0%; p<0.001), smoking (12.9% vs. 51.0%; p<0.001), systemic arterial hypertension (SAH — 65.5% vs. 82.3%; p=0.005), dyslipidemia (58.3% vs. 77.1%; p=0.003), chronic coronary syndrome (36.7% vs. 58.3%; p=0.001), and prior cardiovascular events (15.8% vs. 36.5%; p<0.001) when compared to C1. There was also a higher frequency of females in C2 (82.0% vs. 17.7%; p<0.001). Conclusions: A better cardiovascular morbidity profile was observed in the group of patients with higher R/S levels, suggesting a probable positive relationship between R/S and cardiovascular health.

https://doi.org/10.36660/ijcs.20220176

13.

Impacto do perfil ocupacional, saúde mental e religiosidade sobre depressão, ansiedade e estresse de profissionais de saúde na pandemia de COVID-19
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Miranda, Kamila Silva de

; Santos, Leonardo Daniel Reis

; Almeida Neto, Omar Pereira de

; Raponi, Maria Beatriz Guimarães

; Scalia, Luana Araújo Macedo

Jornal Brasileiro de Psiquiatria

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2023, Volume 72 N. 4 Pages 239 - 246

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective: To evaluate the influence of occupational and mental health variables, religiosity and posttraumatic stress (PTSD) on depression, anxiety and stress in front-line healthcare professionals during the COVID-19 pandemic. Methods: An observational and cross-sectional study was conducted in a hospital in the state of Minas Gerais, Brazil, with 151 health professionals working on the front-line of COVID-19. Sociodemographic, occupational and mental health, religiosity (P-DUREL), PTSD (PCL-C) and screening for depression, anxiety and stress (DASS-21) questionnaires were used. Descriptive, bivariate and multiple linear regression were used. Results: There was a predominance of female, white, Catholic, married, complete higher education, with children, sedentary and without a history of chronic or psychiatric diseases. The minority presented symptoms of COVID-19, tested positive for the virus or needed isolation. Most of them had deprivation of contact with family members and did not receive psychological/psychiatric support. Most professionals had mild or minimal symptoms of depression (68.2%), anxiety (64.9%) or stress (69.5%). The prevalence of PTSD symptoms was 17.2%. A previous history of psychiatric disorder and the presence of PTSD symptoms were associated with current symptoms of depression, anxiety, and stress. Low intrinsic religiosity scores were associated with high depression scores and females were associated with higher depression and stress scores. Conclusions: During the pandemic, symptoms of depression, anxiety and stress were common in this sample of health professionals. The factors associated with these symptoms can be used to identify vulnerable professionals who need psychological/psychiatric support.

RESUMO Objetivo: Avaliar a influência de variáveis ocupacionais e de saúde mental, religiosidade e estresse pós-traumático (TEPT) sobre depressão, ansiedade e estresse em profissionais de saúde da linha de frente da COVID-19. Métodos: Estudo observacional e seccional, realizado em hospital universitário no interior de Minas Gerais, com 151 profissionais de saúde atuantes na linha de frente da COVID-19. Utilizaram-se questionários de caracterização sociodemográfica, ocupacional e de saúde mental; religiosidade (P-DUREL); TEPT (PCL-C) e rastreio de depressão, ansiedade e estresse (DASS-21). Análises descritiva, bivariada e regressão linear múltipla foram empregadas. Resultados: Houve predomínio de participantes de sexo feminino, de cor branca, católicos, casados, de nível superior completo, com filhos, sedentários e sem histórico de doenças crônicas ou psiquiátricas. A minoria apresentou sintomas da COVID-19, teste positivo ou necessidade de isolamento. A maioria teve privação do contato com familiares e não recebeu suporte psicológico/psiquiátrico. A maioria apresentou sintomas leves ou mínimos de depressão (68,2%), ansiedade (64,9%) ou estresse (69,5%). A prevalência de sintomas de TEPT foi de 17,2%. Transtornos psiquiátricos diagnosticados previamente e presença de sintomas de TEPT foram associados a sintomas de depressão, ansiedade e estresse atuais. Escores baixos de religiosidade intrínseca foram associados a escores altos de depressão, e o sexo feminino foi associado a maiores escores de depressão e estresse. Conclusões: Durante a pandemia, sintomas de depressão, ansiedade e estresse foram comuns nessa amostra de profissionais de saúde. Os fatores associados a esses sintomas podem ser utilizados para identificar profissionais vulneráveis que precisem de suporte psicológico/psiquiátrico.

https://doi.org/10.1590/0047-2085000000433

14.

Deep sea ecosystem exploration and ‘health check’: sampling strategy and methods applied during the iAtlantic_BR10_Petrobras cruise in the Santos Basin, Southwest Atlantic
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Perez, Jose Angel A.

; Barros Neto, Halésio M.C. de

; Arantes, Renata C.M.

; Gaurisas, Daniela Y.

; Silva, Camila F.

; Alves, Fernanda Maria M.

; Costa, Júlia A.

; Eloi, Paula D.C.

; Fonseca, Thayse S.

; Gavazzoni, Lucas

; Lonskis, Isabella da S.

; Nardi, Ricardo U.

; Nascimento, Pedro O.

; Rodrigues, João Vitor M.

; Santos, Ana Lara F. dos

; Santos, Edson C. dos

; Schroeder, Rafael

; Silva, Luis Carlos da

; Souza, Fernanda dos S.S. de

; Bernardino, Ângelo F.

; Cavalcanti, Guarani H.

; Lindner, Alberto

; Mahiques, Michel M. de

; Millo, Christian

; Reis, Priscila

; Sweetman, Andrew K.

; Roberts, J. Murray

Ocean and Coastal Research

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2023, Volume 71 elocation e23046

Abstract: En | Text: En | PDF: En

Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.

https://doi.org/10.1590/2675-2824071.23069jaap

15.

Validation of the paper roll plus vermiculite (PR+V) germination test methodology for treated corn seeds
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Rocha, Debora Kelli

; Carvalho, Everson Reis

; Lima, Renato Ribeiro de

; Nardelli, Amanda Carvalho Penido

; Andrade, Thaís de

; Carvalho, Maria Laene Moreira de

; Fernandes, Nilceli

Journal of Seed Science

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2023, Volume 45 elocation e202345026

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract: A germination test is required by Brazilian standards for seed commercialization. The use of an adequate methodology that expresses the real physiological quality of seeds and minimizes possible phytotoxicity by chemicals is important for the production chain. In this context, the aim was to evaluate and validate the paper roll plus vermiculite (PR+V) germination test methodology for use in corn seeds. Two hybrids, one sensitive and the other tolerant to seed treatment, both with different vigor levels and under different chemical treatments, were used. Ten laboratories accredited by the Ministério da Agricultura e Pecuária (MAPA) were selected to perform and evaluate the PR+V germination tests. The data were subjected to statistical procedures to estimate the repeatability and reproducibility and check the accuracy and robustness of the test. The data were accurate, robust, and precise within the critical limits of 1 and 5%. The statistical results for repeatability, reproducibility, and bounds of the Mandel test were also accurate and precise at the critical limits of 1 and 5% for the normal seedlings evaluated by the PR+V methodology. Thus, the methodology can be used routinely to test corn seeds, especially those treated with phytosanitary products.

Resumo: O teste de germinação é exigido pelas normas brasileiras para a comercialização de sementes. O uso de metodologia adequada, que expresse a real qualidade fisiológica das sementes e minimize possível fitotoxidez por produtos químicos é importante para a cadeia produtiva. Nesse contexto, objetivou-se avaliar e validar a metodologia de germinação rolo de papel mais vermiculita (RP+V) para uso em sementes de milho. Utilizou-se dois híbridos, sendo um sensível e outro tolerante ao tratamento de sementes, ambos com diferentes níveis de vigor e tratamentos químicos. Dez laboratórios credenciados pelo Ministério da Agricultura e Pecuária (MAPA) foram selecionados para realização e avaliação do teste de germinação RP+V. Os dados foram submetidos a procedimentos estatísticos para estimar a repetibilidade e a reprodutibilidade e verificar a precisão e robustez do teste. Os dados apresentaram acurácia, robustez e precisão dentro dos limites críticos de 1 e 5%. Os resultados estatísticos para repetibilidade, reprodutibilidade e padrões do teste de Mandel apresentaram exatidão e precisão que estão dentro dos limites críticos de 1 e 5% para plântulas normais avaliadas pela metodologia rolo de papel mais vermiculita. Assim, a metodologia pode ser usada rotineiramente para testar sementes de milho, principalmente para aquelas tratadas com produtos fitossanitários.

https://doi.org/10.1590/2317-1545v45274506

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