Search | SciELO

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

Chemical Characterization of Aspartic Protease from Artichoke Flower (Cynara cardunculus L. var scolymus) Using MIR Spectroscopy and Analytical Measurements
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Jesus, Josane C. de

; Reis, Lucas C. C.

; Santos, Mateus P. F.

; Santos, Leandro S.

; Ferrão, Sibelli P. B.

Journal of the Brazilian Chemical Society

Journal Metrics
- Sobre o periódico
- SciELO Analytics

oct 2023, Volume 34 N. 10 Pages 1520 - 1530

Abstract: En | Text: En | PDF: En

Vegetable proteases have been studied as milk coagulants, and artichoke flower (Cynara cardunculus L. var scolymus) has potential coagulant action as a substitute for microbial chymosin. The objective was to perform chemical characterization of aspartic proteases from artichoke flower (Cynara cardunculus L. var scolymus) by mid-infrared spectroscopy (MIR) and analytical measurements. Artichoke flower extracts were obtained and crude thistle flower extract and microbial chymosin were used as a reference. Plant extracts and microbial chymosin were analyzed for protein concentration, proteolytic activity (PA), milk clotting activity (MCA), specificity ratio (SR), effects of pH, temperature, NaCl and CaCl2 concentration on MCA and PA and characterized by electrophoretic, spectroscopic, chromatographic and storage stability. The results indicated that the crude extract of artichoke flower showed high MCA (510.08 SU mL 1), low PA (10.50 µg mg-1), SR (48.57) and storage stability for up to 90 days under frozen. The electrophoretic profile of artichoke flower resulted in a protein band with an apparent molecular weight of 32 kDa, associated with cardosin A. Artichoke flower may be a new alternative for commercial plant proteases with active enzymes for milk coagulation.

https://doi.org/10.21577/0103-5053.20230065

Internal and external morphology of Tectona grandis Linn F. diaspores and its relation to seed quality
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Oliveira, Andréa dos Santos

; Almeida, Tanismare Tatiana de

; Reis, Rodrigo de Góes Esperon

Ciência Florestal

Journal Metrics
- Sobre o periódico
- SciELO Analytics

sep 2023, Volume 33 N. 3 elocation e71755

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Bearing in mind the importance of Tectona grandis L. production in Mato Grosso State, Brazil, it is necessary to establish crops with seedlings of excellent quality, and the selection of diaspores can improve seed vigor, thus providing better quality to the seedlings. It was aimed to indicate the ideal stage of maturation of T. grandis diaspores through image analysis, guaranteeing seed quality and seedling production. T. grandis diaspores were collected in the years 2016 and 2017 at 90, 120, 150, and 180 days after anthesis. The material collected was analyzed for the presence of seeds per fruit in X-ray equipment to compose the treatments. After this selection, the variables color, area, and diameter were analyzed, and fruit weight, germination, and emergence tests were carried out. The diaspores along the maturation process, had higher amount of seeds and higher weight. The increase in the colors yellow and black was na indicative of the maturation for T. grandis diaspores collected in 2017, and the reduction of the percentagem of dark gray color for the diaspores collected in 2016. Fruit area and diameter was higher in those who had four seeds and the diaspore quality evaluated by the germination percentage, germination first count, initial stand and emergence percentgevary according to the amount of seeds present in the diaspore, being higher for diaspores containing 4 seeds. Diaspore quality was evaluated by germination percentage, first germination count, initial stand, and emergence percentge, was superior in the 2017, with diaspores having 2 na 3 seeds. The maturation of T. grandis diaspores is dependent of the year of collection and presents better quality when collected at 120 and 150 days after anthesis.

RESUMO Tendo em vista a importância da produção de Tectona grandis Linn F. no estado de Mato Grosso, é necessário estabelecer cultivos com mudas de excelente qualidade e a seleção de diásporos pode favorecer seu vigor, proporcionando a produção de mudas de maior padrão. O objetivo da pesquisa foi indicar o estágio de maturação ideal dos diásporos de teca por meio da análise de imagens, que garanta a qualidade de sementes e produção de mudas. Diásporos foram coletados nos anos de 2016 e 2017 aos 90, 120, 150 e 180 dias após a antese e analisados quanto à presença de sementes por fruto em equipamento de raios X para compor os tratamentos. Após a seleção, foram analisadas características de cor, área e diâmetro e realizados testes de peso de frutos, germinação e emergência. Os diásporos durante o processo de maturação possuíam maior peso e quantidade de sementes. O aumento da porcentagem da cor amarela e preta foi indicativo da maturação dos diásporos coletados em 2017 e a redução da porcentagem da cor cinza escuro para o ano de 2016. A área e diâmetro dos frutos foram maiores naqueles que continham quatro sementes e a qualidade dos diásporos avaliados, pela germinação, primeira contagem de germinação, estande inicial e emergência, foi superior no ano de 2017, com diásporos contendo 2 e 3 sementes. A maturação dos diásporos de teca são dependentes do ano de coleta e com melhor qualidade quando coletados aos 120 e 150 dias após a antese.

https://doi.org/10.5902/1980509871755

Salicylic acid on gas exchange and growth of Hymenaea courbaril L. seedlings under flooding
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Santos, Cleberton C.

; Silva, Luis F. P. da

; Piesanti, Matheus

; Scalon, Silvana de P. Q.

; Goelzer, Ademir

; Silverio, Juliana M.

; Reis, Lucas C.

Revista Brasileira de Engenharia Agrícola e Ambiental

Journal Metrics
- Sobre o periódico
- SciELO Analytics

may 2023, Volume 27 N. 5 Pages 327 - 334

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Flooding is a stressful condition that causes damage to the photosynthetic apparatus, negatively affecting the growth of seedlings of fruit species, including Hymenaea courbaril L. Studies on physiological management, such as exogenous application of phytohormones, to alleviate abiotic stress have increased. The objective of this study was to evaluate the effect of foliar application of salicylic acid (SA) on H. courbaril seedlings subjected to flooding. The seedlings received foliar application of four concentrations of SA (0, 100, 200, and 400 mg L-1) and were subjected to two water regimes: a) control - non-flooded and irrigated daily and b) flooded - seedlings were placed in a plastic pool, keeping the water depth at ± 5.0 cm above the substrate level. After 35 days, we evaluated the gas exchange, initial growth, quality, and potential ecological resilience of the seedlings. All flooded seedlings survived, but had reduced photosynthesis, carboxylation of Rubisco efficiency, and water-use and stomatal conductance when they received 100-300 mg L-1 SA. The seedlings that received between 100 and 300 mg L-1 SA had greater growth, quality, and phenotypic plasticity. Foliar application of SA did not mitigated the effect of flooding on gas exchange but contributed to the growth of H. courbaril seedlings.

RESUMO O alagamento é um fator estressante e promove danos ao aparato fotossintético, afetando negativamente o crescimento de mudas de espécies frutíferas, dentre elas a jatobazeiro Estudos sobre o manejo fisiológico, tal como aplicação exógena de fitohormônio, para aliviar o estresse abiótico tem aumentado. Assim, objetivou-se com esse estudo avaliar o efeito da aplicação foliar de concentrações de ácido salicílico (AS) em mudas de H. courbaril submetidas ao alagamento. As mudas receberam aplicação via foliar de quatro concentrações de AS: 0, 100, 200 e 400 mg L-1, e submetidas a dois regimes hídricos: a) controle: - não alagadas e irrigadas diariamente e b) alagamento - acondicionamento das mudas em piscina com lâmina d’água (5.0 cm) acima do nível do substrato. Decorridos 35 dias avaliamos as trocas gasosas, crescimento inicial, qualidade e o potencial de resiliência ecológica das mudas. Observou-se que todas as mudas alagadas sobreviveram, mas tiveram redução da fotossíntese, eficiência instantânea de carboxilação da Rubisco e de uso da água e condutância estomática e quando receberam aplicação entre 100-300 mg L-1 de AS. As mudas que receberam aplicação das concentrações entre 100 e 300 mg L-1 de AS tiveram maiores características de crescimento, qualidade e plasticidade fenotípica. A aplicação foliar de ácido salicílico não mitigou o efeito do alagamento sobre as trocas gasosas, mas contribuiu no crescimento das mudas de H. courbaril.

https://doi.org/10.1590/1807-1929/agriambi.v27n5p327-334

Calcium pyruvate in the attenuation of the water deficit on the agro-industrial quality of ratoon sugarcane
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Dias, M. S.

; Silva, F. A.

; Fernandes, P. D.

; Farias, C. H. A.

; Silva, I. J.

; Silva, M. F. C.

; Lima, R. F.

; Lacerda, C. N.

; Lima, A. M.

; Lima, V. R. N.

; Silva, A. A. R.

; Reis, L. S.

Brazilian Journal of Biology

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2023, Volume 83 elocation e275046

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Sugarcane is one of the largest agricultural commodities when considering the export volume and the number of jobs generated. Sugarcane production in the Brazilian Northeast region is generally low due to several factors, including the irregular rainfall distribution, which highlights the importance of studies aimed at mitigating the deleterious effects of water stress. In this scenario, this study aimed to evaluate calcium pyruvate as a water deficit attenuator on the agro-industrial quality of sugarcane in the second cycle of cultivation. The experiment was conducted out under greenhouse conditions of the Federal University of Campina Grande, where five sugarcane commercial genotypes tested (G1- RB863129, G2- RB92579, G3- RB962962, G4- RB021754, and G5- RB041443) and three irrigation management strategies (E1- full irrigation, E2- water deficit with application of 30 mM of calcium pyruvate, and E3- water deficit without calcium pyruvate application), distributed in randomized blocks in 5 × 3 factorial arrangement with three replications. The RB021754 genotype under water deficit and without foliar application of calcium pyruvate increased the fiber content (13.2%) and the sugarcane moist cake weight (143.5 g). The effects of water deficit in sugarcane genotypes are attenuated by the exogenous application of 30 mM of calcium pyruvate, with benefits on the polarized sucrose content, apparent sucrose content of the juice, soluble solids content, purity, corrected cane POL, total recoverable sugars, and stem mass in relation to plants under water deficit without calcium pyruvate application.

Resumo A cana-de-açúcar é uma das maiores commodities agrícolas, quando se considera o volume de exportação e o número de empregos gerados. A produção no Nordeste brasileiro, em geral, é baixa, decorrente de diversos fatores, entre eles, a irregularidade de distribuição das chuvas, o que realça a importância de estudos com vistas a minorar os efeitos deletérios do estresse hídrico. Nesse cenário, objetivou-se avaliar o piruvato de cálcio como atenuante do déficit hídrico sobre a qualidade agroindustrial da cana-de-açúcar no segundo ciclo de cultivo. O experimento foi conduzido em casa de vegetação da Universidade Federal de Campina Grande, onde foram testados cinco genótipos de cana-de-açúcar (G1- RB863129, G2- RB92579, G3- RB962962, G4- RB021754 e G5- RB041443) submetidos a três estratégias de manejo da irrigação (E1- Irrigação plena, E2- déficit hídrico com aplicação de 30 mM de piruvato de cálcio e E3- déficit hídrico sem aplicação de piruvato de cálcio), distribuídos em blocos casualizados, em esquema fatorial 5 × 3, com três repetições. O genótipo RB021754 sob déficit hídrico e sem aplicação foliar do piruvato de cálcio aumentou o teor de fibra (13,2%) e o peso do bolo úmido da cana (143,5 g). Os efeitos do déficit hídrico em genótipos de cana-de-açúcar são atenuados com aplicação exógena de 30 mM de piruvato de cálcio, com benefícios sobre o teor sacarose polarizada, teor de sacarose aparente do caldo, teor de sólidos solúveis, pureza, pol da cana corrigida, açúcares totais recuperáveis e massa de colmos, em relação as plantas sob déficit hídrico sem aplicação de piruvato de cálcio.

https://doi.org/10.1590/1519-6984.275046

Selecting tropical wheat genotypes through combining ability analysis
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

SILVA, CAIQUE MACHADO E

; NARDINO, MAICON

; MEZZOMO, HENRIQUE C.

; CASAGRANDE, CLEITON RENATO

; LIMA, GABRIEL W.

; SIGNORINI, VICTOR S.

; FREITAS, DAVI S. DE

; BATISTA, CLÁUDIO V.

; REIS, EDÉSIO F. DOS

; BHERING, LEONARDO L.

; OLIVEIRA, ALUÍZIO B. DE

Anais da Academia Brasileira de Ciências

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2023, Volume 95 Suppl. 2 elocation e20220760

Abstract: En | Text: En | PDF: En

Abstract The selection of parents to originate promising base populations, as well as the knowledge of the gene effects controlling agronomic traits by means of diallel, are useful to drive genetic gains in Brazilian tropical wheat breeding programs. The goals of this study were to select tropical wheat parents with a high frequency of favorable alleles and segregating populations with high potential to originate superior progenies through partial diallel analysis. Thus, 14 parents were divided in two groups and crossed in a 7 × 7 partial diallel scheme to originate 49 F1 combinations. After obtaining F2 generation, the populations and the parents were evaluated in the field in the summer of 2021. Days for heading, plant height, rust and yellow spot resistance, and grain yield were evaluated. The data were subjected to partial diallel analysis. There were significant effects of general combining ability for all traits. The specific combining ability effect was significant for days for heading and plant height. The additive gene effects were predominant over the non-additive ones. The parents with the highest frequency of favorable alleles for the traits evaluated were selected in each group. Four populations with high genetic potential to originate superior progenies were selected.

https://doi.org/10.1590/0001-3765202320220760

Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Leonardo-Silva, Lucas

; Silva, Larissa B. da

; Ferreira, Enderson P. de B.

; Reis, Karina F. D. N. S. dos

; Naves, Plínio L. F.

; Martin-Didonet, Claudia C. G.

Revista Caatinga

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2023, Volume 36 N. 4 Pages 775 - 784

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops.

RESUMO O arroz apresenta altos custos de produção no Brasil devido ao elevado uso de agroquímicos. Algumas rizobactérias promotoras de crescimento de plantas (PGPR) podem ser utilizadas como alternativa aos fertilizantes e produtos fitossanitários. Este trabalho teve como objetivo caracterizar bactérias endofíticas obtidas de raízes de arroz. A caracterização dos isolados foi baseada na morfologia da colônia, resistência a antibióticos, uso de fontes de carbono, atividade enzimática (catalase, amilase, protease, celulase e lipase), solubilização de fosfato inorgânico e região intergênica do 16S-23S rDNA. Com base na morfologia, 68% dos isolados apresentaram crescimento rápido, 46% apresentaram produção abundante de muco e 77% apresentaram colônia viscosa. Todos os isolados foram resistentes ao ácido nalidíxico e 16% deles à estreptomicina. A maioria dos isolados (90%) utilizou monossacarídeos e dissacarídeos no ensaio da fonte de carbono. A maioria dos isolados (95%) foi positiva para catalase e 63,6% foram positivas para amilase, protease, lipase e celulase. Além disso, 59% foram capazes de solubilizar o fosfato. A média do índice enzimático para amilase, celulase e protease foi de 2,8, 3,5 e 1,7, respectivamente. A análise de similaridade revelou alta diversidade entre os isolados, com índice de similaridade de 70% (baseado nas características morfológicas) e 60% (baseado na análise da região intergênica 16S-23S rDNA). Com base nas características morfofisiológicas e genotípicas, três isolados promissores devem ser avaliados em estudos futuros em condições de campo, podendo gerar bioprodutos destinados a substituir insumos industrializados na cultura do arroz.

https://doi.org/10.1590/1983-21252023v36n405rc

Hydrogen peroxide is involved in drought stress long-distance signaling controlling early stomatal closure in tomato plants
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Reis, A. D. P.

; Carvalho, R. F.

; Costa, I. B.

; Girio, R. J. S.

; Gualberto, R.

; Spers, R. C.

; Gaion, L. A.

Brazilian Journal of Biology

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2022, Volume 82 elocation e267343

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Tem sido hipotetizado que o peróxido de hidrogênio (H2O2) pode desempenhar um papel essencial na sinalização de longa distância entre a raiz e a parte aérea sob condições de seca. Assim, para melhor entender o envolvimento do H2O2 na sinalização da seca, dois experimentos foram realizados com plantas de tomate. No primeiro, foi utilizado o esquema de raízes divididas, enquanto no segundo, os tomateiros foram cultivados em um único vaso e submetidos ao déficit hídrico. Em ambos os experimentos, o H2O2 e a catalase foram aplicados juntamente com a irrigação. As plantas do grupo controle continuaram a ser irrigadas de acordo com a perda de água. No experimento de raiz dividida, verificou-se que a aplicação de H2O2 nas raízes induziu uma clara redução na transpiração da planta em comparação com plantas não tratadas ou tratadas com catalase. No segundo experimento, observamos que plantas tratadas com H2O2 apresentaram transpiração semelhante quando comparadas com plantas não tratadas e tratadas com catalase sob seca. Da mesma forma, não foi observada diferença na eficiência do uso da água. Assim, concluímos que o aumento do H2O2 no sistema radicular pode atuar como um sinal de longa distância levando à redução da transpiração mesmo quando não há limitação hídrica na parte aérea. Mas tem pouco efeito quando há redução do potencial hídrico da parte aérea.

Abstract It has long been hypothesized that hydrogen peroxide (H2O2) may play an essential role in root-to-shoot long-distance signaling during drought conditions. Thus, to better understand the involvement of H2O2 in drought signaling, two experiments were carried out using tomato plants. In the first experiment, a split-root scheme was used, while in the second experiment, the tomato plants were grown in a single pot and subjected to drought stress. In both experiments, H2O2 and catalase were applied together with irrigation. Control plants continued to be irrigated according to the water loss. In the split-root experiment, it was verified that the application of H2O2 to roots induced a clear reduction in plant transpiration compared to untreated or catalase-treated plants. In the second experiment, we observed that H2O2-treated plants exhibited similar transpiration when compared to untreated and catalase-treated plants under drought stress. Similarly, no difference in water use efficiency was observed. Thus, we conclude that the increase in H2O2 in the root system can act as a long-distance signal leading to reduced transpiration even when there is no water limitation in the shoot. But it has little effect when there is a reduction in the shoot water potential.

https://doi.org/10.1590/1519-6984.267343

Phytochemical characterization, and antioxidant and antibacterial activities of the hydroethanolic extract of Anadenanthera peregrina stem bark
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Marinho, T. A.

; Oliveira, M. G.

; Menezes-Filho, A. C. P.

; Castro, C. F. S.

; Oliveira, I. M. M.

; Borges, L. L.

; Melo-Reis, P. R.

; Silva-Jr, N. J.

Brazilian Journal of Biology

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2022, Volume 82 elocation e234476

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O bioma Cerrado brasileiro apresenta em uma grande variedade de espécies endêmicas com diversos compostos bioativos, e Anadenanthera peregrina (L.) Speg é uma espécie promissora. Neste estudo, objetivamos realizar a caracterização fitoquímica e avaliar as atividades antioxidantes e antibacterianas contra Staphylococcus aureus e Escherichia coli do extrato hidroetanólico de cascas do caule de A. peregrina. As cascas foram coletadas no Jardim Botânico de Goiânia, Brasil. O extrato hidroetanólico foi obtido por percolação e submetido a triagem físico-química, estimativa de conteúdo fenólico total, impressão digital por cromatografia líquida de alta eficiência (HPLC) e determinação da atividade antioxidante (valores de IC50 foram calculados para o ensaio 2,2-difenil-1-picril-hidrazil) e antibacteriana. O pH do extrato foi de 5,21 e a densidade foi de 0,956 g/cm3. A triagem fitoquímica indicou a presença de glicosídeos cardíacos, ácidos orgânicos, açúcares redutores, saponinas hemolíticas, fenóis, cumarinas, taninos condensados, flavonóides, catequinas, depsídios e depsidonas derivados de benzoquinonas. O extrato mostrou intensa atividade hemolítica. O conteúdo fenólico total foi de 6,40 g de GAE 100 g-1. A análise por impressão digital por HPLC revelou a presença de ácido gálico, catequina e epicatequina. Confirmamos a atividade antioxidante do extrato. Além disso, o extrato não inibiu o crescimento de colônias de E. coli em nenhum volume testado, mas houve halos em torno das colônias de S. aureus nos três volumes testados. Estes resultados contribuem para uma melhor compreensão da composição química da casca de A. peregrina e apoia ainda mais as aplicações medicinais desta espécie.

Abstract The Brazilian Cerrado biome consists of a great variety of endemic species with several bioactive compounds, and Anadenanthera peregrina (L.) Speg is a promising species. In this study, we aimed to perform phytochemical characterization and evaluate the antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli of the hydroethanolic extract of A. peregrina stem bark. The barks were collected in the Botanical Garden of Goiânia, Brazil. The hydroethanolic extract was obtained by percolation and subjected to physicochemical screening, total phenolic content estimation, high-performance liquid chromatography (HPLC) fingerprinting, and antioxidant (IC50 values were calculated for the 2,2-diphenyl-1-picrylhydrazyl assay - DPPH) and antibacterial activity determination. The pH of the extract was 5.21 and density was 0.956 g/cm3. The phytochemical screening indicated the presence of cardiac glycosides, organic acids, reducing sugars, hemolytic saponins, phenols, coumarins, condensed tannins, flavonoids, catechins, depsides, and depsidones derived from benzoquinones. The extract showed intense hemolytic activity. The total phenolic content was 6.40 g GAE 100 g-1. The HPLC fingerprinting analysis revealed the presence of gallic acid, catechin, and epicatechin. We confirmed the antioxidant activity of the extract. Furthermore, the extract did not inhibit the growth of E. coli colonies at any volume tested, but there were halos around S. aureus colonies at all three volumes tested. These results contribute to a better understanding of the chemical composition of A. peregrina stem bark and further support the medicinal applications of this species.

https://doi.org/10.1590/1519-6984.234476 447 downloads

10.

Natural history museums and zoological collections of São Paulo State
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Percequillo, Alexandre Reis

; Barbosa, Marina F. C.

; Bockmann, Flávio A.

; Bogoni, Juliano A.

; Esguícero, André L. H.

; Lamas, Carlos

; Moraes, Gilberto José de

; Pinto-da-Rocha, Ricardo

; Silveira, Luís Fábio

Biota Neotropica

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2022, Volume 22 N. spe elocation e20221426

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Scientific collections constitute a valuable source for contributions to scientific research and the training of human resources in systematics, but also other areas of biological knowledge. In this contribution, we intend to discuss these advancements in collections and the role played by FAPESP in sponsoring them, as well as a general overview of the zoological collections in São Paulo state. We also aim to stress the importance of zoological collections and the need for continuous logistic and financial support from institutions and research agencies to maintain and develop these unique repositories of biodiversity. From 1980 to the present, FAPESP supported 118 research projects focused on several areas of zoology that are directly or indirectly associated with collections. There is a constant growth in the number of projects, and the financial support provided by FAPESP through the Biota Program was paramount for the advancement of our knowledge of biodiversity in Brazil. Parallel to the scientific advances, but not less important, this support allowed curators to increase the number of specimens, and to organize, maintain and digitize them in these valuable and irreplaceable collections. Regarding the lack of new taxonomists, it is essential that FAPESP and universities in São Paulo encourage the formation of new academics in zoological groups where specialists are rare. Considering the investment provided by FAPESP, it is quite important that the institutions that benefited from these resources took greater responsibility to safeguard these collections, and they should consider including resources on their budgets to obtain safety certificates, ensuring their permanence for many generations to come. Zoological collections are a heritage of humanity and are essential not only for the improvement of our knowledge of biodiversity but also with direct applications, among other services provided by these biological resources. It is important that research and teaching institutions in São Paulo that house specimens under their care start to value more this important patrimony and this heritage, as these collections represent the most valuable testimony of our impressive biodiversity, records of our past, and windows to our future, essential to our academic, scientific, cultural and social sovereignty.

Resumo As coleções científicas constituem uma fonte valiosa para contribuições à pesquisa científica e para a formação de recursos humanos em sistemática, mas também em outras áreas do conhecimento biológico. Nesta contribuição, pretendemos discutir esses avanços nas coleções e o papel desempenhado pela FAPESP no seu patrocínio, bem como um panorama geral das coleções zoológicas do estado de São Paulo. Também pretendemos enfatizar a importância das coleções zoológicas e a necessidade de apoio logístico e financeiro contínuo de instituições e agências de pesquisa para manter e desenvolver esses repositórios únicos de biodiversidade. Ao longo de 1980 até os dias atuais, a FAPESP apoiou 118 projetos de pesquisa focados em diversas áreas da zoologia, direta ou indiretamente associados a coleções. Há um crescimento constante no número de projetos, e o apoio financeiro da FAPESP por meio do Programa Biota foi fundamental para o avanço do nosso conhecimento sobre a biodiversidade no Brasil. Paralelamente aos avanços científicos, mas não menos importante, este apoio permitiu aos curadores aumentar o número de exemplares, e organizá-los, mantê-los e digitalizá-los nestas valiosas e insubstituíveis coleções. Em relação à falta de novos taxonomistas, é fundamental que a FAPESP e as universidades paulistas estimulem a formação de novos acadêmicos em grupos zoológicos onde os especialistas são raros. Considerando o investimento realizado pela FAPESP, é de suma importância que as instituições beneficiadas com esses recursos tenham maior responsabilidade na salvaguarda desses acervos, devendo considerar a inclusão de recursos em seus orçamentos para obtenção de certificados de segurança, garantindo sua permanência por muitas gerações. As coleções zoológicas são patrimônio da humanidade, e são essenciais não apenas para o aprimoramento do nosso conhecimento sobre a biodiversidade, mas também com aplicações diretas, entre outros serviços prestados por esses recursos biológicos. É importante que as instituições de pesquisa e ensino paulistas que abrigam exemplares sob seus cuidados passem a valorizar mais esse importante patrimônio e essa herança, pois essas coleções representam o testemunho mais valioso de nossa impressionante biodiversidade, registros do nosso passado e janelas para o nosso futuro, essenciais à nossa soberania acadêmica, científica, cultural e social.

https://doi.org/10.1590/1676-0611-bn-2022-1426

11.

Low- and high-viscosity bulk-fill resin composites
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics

França, Fabiana M. G.

; Tenuti, Jonathan G. B.

; Broglio, Isabela P.

; Paiva, Lara E. J.

; Basting, Roberta T.

; Turssi, Cecília P.

; Amaral, Flávia L. B. do

; Reis, André F.

; Vieira-Junior, Waldemir F.

Acta Odontológica Latinoamericana

Journal Metrics
- Sobre o periódico
- SciELO Analytics

jun 2021, Volume 34 N. 2 Pages 173 - 182

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste estudo foi comparar resinas compostas bulk- -fill de baixa e alta viscosidade quanto à microdureza Knoop (KHN), resistência de união a microtração (MTBS) em cavidades oclusais e carga à fratura (FS) em molares com restauração mesio-oclusal-distal. Amostras em forma de disco com diferentes espessuras (2 ou 4 mm) de resinas bulk-fill de baixa viscosidade (SDR Flow, Dentsply) e alta viscosidade (Filtek BulkFill, 3M ESPE; e Tetric-N Ceram Bulk Fill, Ivoclar Vivadent) foram obtidas para análise de KHN no topo e na base (n = 10). A MTBS em dentina e o padrão de fratura foram avaliados em molares humanos com cavidades oclusais restauradas com (n = 10): resina composta nanoparticulada convencional (Z350XT, 3M ESPE), resinas bulk-fill de baixa viscosidade (Filtek Bulk-fill Flow, 3M ESPE) ou alta viscosidade (Filtek BulkFill). Foram investigados a FS e o padrão de fratura de molares humanos em restaurações mesial-ocluso-distais sub metidas ou não à ciclagem termomecânica (n = 10), sendo: dente íntegro (controle), e restaurações baseadas em resina composta microhíbrida convencional (Z250, 3M ESPE); resinas bulk-fill de baixa viscosidade (SDR Flow) ou alta viscosidade (Filtek BulkFill). Os dados foram submetidos a split-plot ANOVA (KHN), one-way ANOVA (MTBS), two-way ANOVA (FS) seguidos do teste de Tukey (α = 0,05). Para KHN, não houve diferença significativa entre o topo e a base para as resinas compostas. Para MTBS, não foram detectadas diferenças significativas entre os materiais; entretanto, a resina bulk-fill de baixa viscosidade apresentou menor frequência de falhas adesivas. Para FS, não houve diferença significativa entre os materiais e o dente íntegro, independentemente da ciclagem termomecânica. As resinas bulk-fill de baixa e alta viscosidade têm microdureza e resistência à microtração comparáveis quando usados em restaurações oclusais. Da mesma forma, as resinas bulk-fill apresentam resistência à fratura semelhante em molares com restaurações mesio-oclusal-distais.

ABSTRACT The aim of this study was to compare low- and high-viscosity bulk-fill composites for Knoop microhardness (KHN), microtensile bond strength (MTBS) to dentin in occlusal cavities, and fracture strength (FS) in molars with mesialocclusal- distal restoration. Disk-shaped samples with different thicknesses (2 or 4 mm) of low-viscosity (SDR Flow, Dentsply) and high-viscosity bulk-fill composites (Filtek BulkFill, 3M ESPE; and Tetric-N Ceram Bulk Fill, Ivoclar Vivadent) were prepared for top and bottom KHN analysis (n=10). MTBS to dentin and fracture pattern was evaluated in human molars with occlusal cavities restored with (n=10): conventional nanocomposite (Z350XT, 3M ESPE), low-viscosity (Filtek Bulk-fill Flow, 3M ESPE) or high-viscosity bulk-fill composites (Filtek BulkFill). The FS and fracture pattern of human molar with mesial-occlusal-distal restorations submitted or not to thermomechanical cycling were investigated (n=10) using: intact tooth (control), and restoration based on conventional microhybrid composite (Z250, 3M ESPE), low-viscosity (SDR Flow) or high-viscosity bulk-fill composites (Filtek BulkFill). The data were submitted to split-plot ANOVA (KHN), one-way ANOVA (MTBS), two-way ANOVA (FS) followed by Tukey’s test (α=0.05). For KHN, there was no significant difference for the resin composites between the top and bottom. For MTBS, no significant differences among the materials were detected; however, the low-viscosity composite presented lower frequency of adhesive failures. For FS, there was no significant difference between composites and intact tooth regardless of thermomechanical cycling. Low- and high-viscosity bulk-fill composites have comparable microhardness and microtensile bond strength when used in occlusal restorations. Likewise, the bulk-fill composites present similar fracture strength in molars with mesio-occlusal-distal restorations.

219 downloads

12.

Management of trickle irrigation for banana: Hydrodynamic processes and sensor placement at the root zone
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Santana Junior, Edvaldo B.

; Coelho, Eugênio F.

; Santos, Marcelo R. dos

; Silva, Alisson J. P. da

; Reis, João B. R. da S.

; Pereira, Bruno L. da S.

Revista Brasileira de Engenharia Agrícola e Ambiental

Journal Metrics
- Sobre o periódico
- SciELO Analytics

may 2021, Volume 25 N. 5 Pages 312 - 318

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O conhecimento do processo hidrodinâmico no solo ajuda a explicar a relação entre solo-água-planta com efeitos práticos no manejo da irrigação assim como no posicionamento de sensores de umidade no solo. O estudo foi realizado com o objetivo de detalhar o processo hidrodinâmico na zona radicular e definir a localização para o posicionamento do sensor de umidade do solo sob diferentes configurações de irrigação localizada na cultura da bananeira. Foram avaliados três microaspersores com vazões de 70 (T1), 53 (T2), 35 L h-1 (T3) e dois sistemas de gotejamento, (T4) - uma linha de gotejamento por linha de plantas, (T5) - duas linhas de gotejamento por linha de plantas. O experimento seguiu um delineamento em blocos casualizados consistindo de cinco tratamentos com cinco repetições. A extração de água foi maior nos sistemas de irrigação com maiores taxas de vazão para todas as configurações de sistemas de irrigação localizada. Os sensores de umidade do solo em sistemas de gotejamento devem estar a distâncias de 0,75 a 0,81m do pseudocaule e em profundidades de 0,33 a 0,44 m. Em sistemas de microaspersão, os sensores de umidade do solo devem estar a 0,75, 0,77 e 0,83 m do pseudocaule para o emissor e a profundidades de 0,33, 0,48 e 0,55 m para vazões de 35, 53 e 70 L h-1, respectivamente.

ABSTRACT Information on soil hydrodynamic processes assists in explaining the soil-water-plant relationship and has practical applications to irrigation management, such as the definition of soil water sensor placement. The objective of this study was to detail the hydrodynamic process in the soil root zone and to define the location for placement of soil water sensor under different configurations of trickle irrigation in banana crops. Three micro-sprinkler emitters with flow rates of 70 (T1), 53 (T2), 35 L h-1 (T3), and two drip system, one with one drip line per row of plants (T4), and another with two drip lines per row of plants (T5) were evaluated. The experiment was conducted in a randomized block design with five repetitions. Higher water extraction was found for irrigation systems with higher flow rates for all configurations of trickle irrigation systems. Soil moisture sensors in drip systems should be placed at distances of 0.75 to 0.81 m from the pseudo stem and at depths of 0.33 to 0.44 m. Under micro-sprinkler systems, soil water sensors should be placed at 0.75, 0.77 and 0.83 m from the pseudo stem towards to the emitter and at depths of 0.33, 0.48 and 0.55 m for emitter flow rates of 35, 53 and 70 L h-1, respectively.

https://doi.org/10.1590/1807-1929/agriambi.v25n5p312-318 305 downloads

13.

Modelagem matemática e propriedades termodinâmicas na secagem do quiabo
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Santos*, Francislaine S.

; Figueirêdo, Rossana M. F.

; Queiroz, Alexandre J. M.

; Reis, Carolaine G.

; Amadeu, Lumara T. S.

; Silva, Luis P. F. R.

; Lima, Thalis L. B.

Revista de Ciências Agrárias

Journal Metrics
- Sobre o periódico
- SciELO Analytics

mar 2021, Volume 44 N. 1 Pages 91 - 100

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O quiabo é uma hortaliça que destaca-se agronomicamente por apresentar ciclo vegetativo rápido, de alto rendimento, além de apresentar considerável valor nutritivo. Em razão da sua composição e de ser consumido ainda verde, a sua vida útil é bastante curta, requerendo algum processo de conservação para manter a aceitabilidade. Nesse sentido, objetivou-se secar o quiabo em fatias, com espessura de aproximadamente 2 mm, nas temperaturas de 50, 60, 70 e 80 °C, ajustar modelos matemáticos aos dados experimentais e determinar os coeficientes de difusão, a energia de ativação e as propriedades termodinâmicas. Observou-se que o aumento de temperatura de secagem em 30 °C reduziu em 50,55% o tempo total de processo. Dentre os modelos matemáticos testados, o modelo de Midilli apresentou os melhores parâmetros de ajustes aos dados experimentais. Os coeficientes de difusão efetivos aumentaram com o incremento da temperatura, apresentando-se na ordem de 10-11 m2 s-1, e sua dependência com a temperatura foi descrita pela Equação de Arrhenius, que indicou uma energia de ativação de 26,12 kJ mol-1. Os valores de entalpia e entropia se reduziram com a elevação da temperatura de secagem, enquanto que a energia livre de Gibbs foi aumentada na faixa de temperatura avaliada.

ABSTRACT Okra is a vegetable that stands out agronomically due to its fast vegetative cycle, of high yield, besides presenting considerable nutritional value. Due to its composition and to be consumed still green, its useful life is quite short, requiring some conservation process to maintain acceptability. In this sense, the objective was to dry the okra in slices, approximately 2 mm thick, at temperatures of 50, 60, 70 and 80 ° C, adjust mathematical models to the experimental data and determine the diffusion coefficients, activation energy and thermodynamic properties. It was observed that the increase in drying temperature at 30 ° C reduced the total process time by 50.55%. Among the mathematical models tested, the Midilli model presented the best parameters of adjustments to the experimental data. The effective diffusion coefficients increased with increasing temperature, in the order of 10-11 m2 s-1, and their dependence on temperature was described by the Arrhenius Equation, which indicated an activation energy of 26.12 kJ mol-1. The enthalpy and entropy values decreased with the increase of the drying temperature, while the Gibbs free energy was increased in the temperature range evaluated.

https://doi.org/10.19084/rca.21469 4 downloads

14.

Talinumpaniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Cerdeira, Cláudio Daniel

; Silva, Jeferson J. da

; Netto, Manoel F. R.

; Boriollo, Marcelo F. G.

; Moraes, Gabriel O. I.

; Santos, Gérsika B.

; dos Reis, Luis F. C.

; Brigagão, Maísa R. P. L.

Revista Colombiana de Ciencias Químico - Farmacéuticas

Journal Metrics
- Sobre o periódico
- SciELO Analytics

aug 2020, Volume 49 N. 2 Pages 401 - 431

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Objetivos: este estudio investigó la bioactividad del extracto de hoja en bruto (EHB) y las fracciones hexano (HX) y acetato de etilo (AcOEt) de Talinum paniculatum solo y en asociación con fluconazol (FLC) contra cepas de referencia y aislados clínicos de Candida albicans resistente a FLC. Además, evaluó la capacidad antioxidante, la composición química de esta planta y los mecanismos subyacentes del efecto fungicida. Métodos: la actividad antifúngica se evaluó mediante microdilución en caldo para establecer las concentraciones inhibitorias mínimas (CIM) y microbicidas mínimas (CMM). Durante el tratamiento con FLC y productos vegetales se detectó la generación de especies reactivas de oxígeno (ERO) (radicales hidroxilo [HO●]) en células de C. albicans utilizando las sondas fluorescentes permeables a la membrana APF y HPF. El perfil de cromatografía líquida de alta resolución (CLAR), el análisis cuantitativo de compuestos antioxidantes y el ensayo DPPH fueron evaluados. Resultados: el EHB y las fracciones presentaron una excelente actividad antifúngica y selectividad contra las células de C. albicans, pero no tuvieron efectos sinérgicos con FLC. Los valores de CIM para EHB y sus fracciones contra la cepa referencia de C. albicans fueron del orden de: HX (31,25 μg ml-1) < AcOEt (62,5 μg ml-1) < EHB (500 μg ml-1), y contra C. albicans resistente a FLC: HX (125 μg ml-1)= AcOEt < EHB (500 µg ml-1). EHB y sus fracciones fueron más potentes antifúngicos que FLC contra los aislados clínicos. Además, estos productos vegetales tienen efectos fungicidas contra C. albicans resistentes a FLC, esto conirmó el potencial antifúngico. Por el contrario, durante la asociación se demostró que los productos vegetales causan un aumento en la CIM de FLC de 2 a 16 veces. La exposición a FLC aumentó los niveles de ERO (HO●) en las células de C. albicans. Los aumentos en las CIM de FLC se debieron a la acción de los antioxidantes presentes en EHB y sus fracciones para prevenir la inhibición del crecimiento mediada por ERO inducida por FLC en C. albicans. Conclusión: T. paniculatum puede ser una fuente de compuestos bioactivos con potencial antifúngico. Sin embargo, debido al uso común de su hoja comestible, se recomienda usarla con precaución durante la terapia con FLC (ya que puede disminuir la susceptibilidad a FLC).

SUMMARY Aims: This study investigated the bioactivity of the crude leaf extract (CLE) and fractions hexane (HX) and ethyl acetate (EtOAc) from Talinum paniculatum alone and in association with fluconazole (FLC) against reference strain and clinical isolates of FLC-resistant Candida albicans. Furthermore, the antioxidant capability, chemical composition of this plant, and the effect's underlying mechanisms were evaluated. Methods: The antifungal activity was evaluated using checkerboard assay to establish the minimum inhibitory (MIC) and minimum microbicidal concen trations (MMC). During FLC and plant products challenges, the reactive oxygen species (ROS) generation (hydroxyl radicals [HO●]) were detected in C. albicans cells using the membrane-permeable fluorescent probes APF and HPF. High-performance liquid chromatography (HPLC) profile, quantitative analysis of antioxidant compounds, and free radical scavenging activity (DPPH assay) tests were performed. Results: The CLE and fractions presented outstanding antifungal activity and selectivity against C. albicans cells but had no synergistic effect’s with FLC. The MIC values for CLE and its fractions against C. albicans reference strain were in the order of HX (31.25 µg ml-1) < EtOAc (62.5 μg ml-1) < CLE (500 μg ml-1), and against FLC-resistant C. albicans HX (125 μg ml-1) = EtOAc < CLE (500 μg ml-1). CLE and its fractions had more potent antifungal activities than FLC against the clinical isolates. Moreover, fungicidal effect’s for these plant products were demonstrated against FLC-resistant C. albicans, which further conirmed an antifungal potential. Conversely, during association, plant products were shown to cause an increase in FLC MIC anywhere from 2- to 16-fold. FLC exposure led to an increase in the steady-state levels of ROS (HO●) in C. albicans cells. Next, we found that the increases in FLC MICs were owing to action of antioxidants containing-CLE and its fractions in preventing FLC-induced ROS-mediated growth inhibition of C. albicans. Conclusion: T. paniculatum can be a source of bioactive compounds with antifungal potential. However, because of the common use of its edible leaf, caution is advised during therapy with FLC (since it can decrease FLC susceptibility).

https://doi.org/10.15446/rcciquifa.v49n2.89704 347 downloads

15.

Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere with oxacillin action
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Cerdeira, Cláudio Daniel

; da Silva, Jeferson J.

; Netto, Manoel F. R.

; Boriollo, Marcelo F. G.

; Santos, Gérsika B.

; dos Reis, Luis F. C.

; Brigagão, Maísa R. P. L.

Revista Colombiana de Ciencias Químico - Farmacéuticas

Journal Metrics
- Sobre o periódico
- SciELO Analytics

aug 2020, Volume 49 N. 2 Pages 432 - 451

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Propósito: evaluamos el potencial antibacteriano del extracto de hoja en bruto (EHB) y las fracciones hexano (HX) y acetato de etilo (AcOEt) de Talinum paniculatum solo y en asociación con oxacilina (OXA) contra Staphylococcus aureus resistente a OXA (ORSA, ambientales) y S. aureus sensible a OXA (OSSA, ATCC 25923). Además, se realizaron pruebas de toxicidad. Métodos: la actividad antibacteriana se evaluó mediante microdilución en caldo para establecer las concentraciones inhibitorias mínimas (CIM) y bactericidas mínimas (CBM). Se evaluó la toxicidad en ratones. Resultados: los valores de CIM para el EHB y sus fracciones contra ORSA y OSSA fueron del orden de HX (500 μg ml-1) = AcOEt < EHB (4000 μg ml-1). AcOEt y HX presentaron actividades antibacterianas sobresalientes contra ORSA, y estas fracciones fueron bactericidas hacia OSSA. Por el contrario, las asociaciones entre el producto vegetal (EHB, AcOEt o HX) y OXA no mostraron efectos sinérgicos. Durante estas asociaciones, hubo un aumento en las CIM de OXA de 2 a 4092 veces. EHB no mostró toxicidad a una dosis de 5 g kg-1. Conclusión: aunque T. paniculatum es una buena fuente de compuestos bioactivos con potencial antiestofilocócico, los investigadores deben ser cautelosos, ya que su hoja comestible puede interferir con la terapia con OXA (mitigando la inhibición del crecimiento inducida por OXA o la muerte de S. aureus y promoviendo resistencia bacteriana).

SUMMARY Propose: We evaluated the antibacterial potential of the crude leaf extract (CLE) and fractions hexane (HX) and ethyl acetate (EtOAc) from Talinum paniculatum alone and in association with oxacillin (OXA) against OXA-resistant Staphylococcus aureus (ORSA, environment isolates) and OXA-sensitive S. aureus (OSSA, ATCC 25923). Furthermore, toxicity tests were performed. Methods: The antibacterial activity was evaluated through checkerboard assay (broth microdilution) to establish the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC). Toxicity test in mice was assessed. Results: The MIC values for the CLE and its fractions against ORSA and OSSA were in the order of HX (500 μg ml-1) = EtOAc < CLE (4000 μg ml-1). EtOAc and HX presented outstanding antibacterial activities against ORSA, and these fractions were bactericidal toward OSSA. Conversely, the associations between plant product (CLE, EtOAc, or HX) and OXA exhibited no synergistic effects. During these associations, there was an increase in OXA MICs anywhere from 2- to 4092-fold. The CLE presented absence of toxicity at a dose of 5 g kg-1 (in vivo). Conclusion: Although T. paniculatum be a good source of bioactive compounds with antistaphylococcal potential, the researchers should be cautious, since its edible leaf may interfere with OXA therapy (mitigating OXA-induced growth inhibition or killing of S. aureus and enhancing S. aureus resistance).

https://doi.org/10.15446/rcciquifa.v49n2.89894 322 downloads

Showing

itens per page

Page of 7 Next

Select one or more results

All Alphabetic

Results

Comparative chart Download CSV

Statistics of

Send result

To*

Use ; (semicolon) to insert more emails.

Send to email

This page

Selected records

All references (max. 300)

Your email

Subject

Comments

Export format:

RIS (for EndNote, ProCite, Reference Manager, RefWorks, Citavi, etc.)

BibTeX

Citation

CSV (for Excel, etc)

Export:

This page

Selected itens

All references (max. 2000)

Sem resultados

No documents were found for your search

Glossary and search help

You can enrich your search in a very simple way. Use the search indexes combined with the connectors (AND or OR) and specify more your search.

For example, if you want to search for articles about
cases of dengue in Brasil in 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

See below the complete list of search indexes that can be used:

Index code	Element
ti	article title
au	author
kw	article keywords
subject	subject (title words, abstract and keywords)
ab	abstract
ta	journal short title (e.g. Cad. Saúde Pública)
journal_title	journal full title (e.g. Cadernos de Saúde Pública)
la	publication language code (e.g. pt - Portuguese, es - Spanish)
type	document type
pid	publication identifier
publication_year	publication year of publication
sponsor	sponsor
aff_country	country code of the author's affiliation
aff_institution	author affiliation institution
volume	article volume
issue	article issue
elocation	elocation
doi	DOI number
issn	journal ISSN
in	SciELO colection code (e.g. scl - Brasil, col - Colômbia)
use_license	article usage license code

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
Email: scielo@scielo.org

All the contents of this site www.scielo.br, except where otherwise noted, is licensed under a Creative Commons Attribution License.
more »