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Abstract Background Evaluation and treatment of primary and secondary headaches is a global public health challenge. Recognizing the epidemiological impact of headaches, a group of researchers linked to the Brazilian Headache Society proposed the Brazilian Headache Registry and drew up its initial protocol. Objective Here we describe the methods and preliminary data obtained from the pilot study. Methods This was a multicenter longitudinal observational study conducted between September 2020 and August 2021. Prospective data were collected in three specialist centers for headache care in states in southern and southeastern Brazil. Patients aged 18 years or older who sought care for headache in tertiary centers and who agreed to participate in the study, were considered eligible. Results Sixty-six patients were included in the pilot study: 43 (65%) from Rio Grande do Sul state and 23 (35%) from Minas Gerais state. Overall, 90% were female, and the subjects' mean age was 38.2 ± 11.2 years. Primary headaches accounted for 85.3% of the diagnoses made. Among secondary headaches, medication overuse headache was the most frequent type (7.1%). Conclusions The pilot study showed the feasibility of the research protocol developed for tertiary centers. The Brazilian Headache Registry will form a source of longitudinal data with the aim of contributing to better characterization of the various phenotypes of patients with primary and secondary headaches, and to detailing the use of health resources and identifying predictors of better clinical outcomes.

Resumo Antecedentes A avaliação e o tratamento das cefaleias primárias e secundárias são um desafio global de saúde pública. Reconhecendo o impacto epidemiológico das cefaleias, um grupo de pesquisadores vinculados à Sociedade Brasileira de Cefaleia propôs a criação de um Registro Brasileiro de Cefaleia e elaborou seu protocolo inicial. Objetivo Nesta publicação descrevemos os métodos e dados preliminares obtidos a partir do estudo piloto. Métodos Trata-se de um estudo prospectivo observacional longitudinal multicêntrico, realizado entre setembro de 2020 e agosto de 2021. Foram coletados dados em três centros especializados no atendimento de cefaleia, em estados da região sul e sudeste do Brasil. Pacientes com idade igual ou superior a 18 anos que procuraram os centros terciários por queixa de cefaleia e concordaram em participar do estudo foram considerados elegíveis. Resultados Sessenta e seis pacientes foram incluídos no estudo piloto, 43 (65%) do Rio Grande do Sul e 23 (35%) de Minas Gerais. Da amostra total, 90% eram do sexo feminino e a idade média dos sujeitos foi de 38,2 ± 11,2 anos. As cefaleias primárias representaram 85,3% dos diagnósticos realizados. Entre as cefaleias secundárias, a cefaleia por uso excessivo de medicamentos foi a mais frequente (7,1%). Conclusões O estudo piloto evidenciou a viabilidade do protocolo de pesquisa desenvolvido para centros terciários. O Registro Brasileiro de Cefaleia constituirá uma fonte de dados longitudinais com o objetivo de contribuir para melhor caracterização dos diversos fenótipos de pacientes com cefaleias primárias e secundárias, detalhar o uso de recursos de saúde e identificar preditores de melhores desfechos clínicos.

https://doi.org/10.1055/s-0043-1771175

Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus
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Rocha, Gabriela Teodoro

; Montalvão, Sandro Coelho Linhares

; Queiroz, Paulo Roberto Martins

; Berçot, Marcelo Rodrigues

; Gomes, Ana Cristina Meneses Mendes

; Monnerat, Rose Gomes

Revista Ceres

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jun 2023, Volume 70 N. 3 Pages 91 - 104

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of this work was to characterize reference bacteria strains, belonging to the genus Bacillus and species of correlated genera, by simplified morphological and biochemical methods. The morphological characterization is based on the aspects of the colonies, as well as cytomorphology of the species, by optical and scanning microscopy. For biochemical characterization, the sensitivity test to antimicrobials by disk-diffusion is performed. Moreover, the strains were characterized by extracting intracellular proteins. Characteristics such as shape, color, and consistency of the colonies, in addition to the type of spore and production of protein crystals were determinants for the morphological characterization of these species. The antibiogram revealed high resistance to β-lactam group antibiotics, in species of Bacillus cereus s.l group. In Bacillus subtilis s.l. group there was high susceptibility to antibiograms, mainly for species of B. subtilis. The protein profile provided specific protein patterns for some species, mainly bands of 130 e 65 kDa for B. thuringiensis, 140 e 130 kDa for Lysinibacillus sphaericus, and 115 kDa for Brevibacillus laterosporus. Our results showed that the morphological and biochemical characterizations, provided a simple identification, with easy interpretation, and low cost.

https://doi.org/10.1590/0034-737x202370030010

Prediction and reasons for COVID-19 second dose vaccine hesitation: a cross-sectional study in a municipality of Brazil
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Sartorão-Filho, Carlos Izaias

; Zoqui, Mariana Costa

; Duarte, Douglas Otomo

; Ribeiro, Edy Alyson

; Bisetto, Vinicius César Queiroz

; Cachoni, Lara Escobar Gavião

; Sartorão, Ana Luísa Varrone

; Terribile, Diogo Coutinho

; Mello, Beatriz Balsimelli de

; Sartorão-Neto, Carlos Izaias

; Mello, Roberto de

Sao Paulo Medical Journal

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2023, Volume 141 N. 3 elocation e202295

Abstract: En | Text: En | PDF: En

ABSTRACT BACKGROUND: Hesitation and refusal to take a second dose of the vaccine for coronavirus disease 19 (COVID-19) are prevalent. OBJECTIVES: We aimed to identify predictive factors for hesitation or refusal and describe groups with higher rates of vaccine hesitancy. DESIGN AND SETTING: A cross-sectional study in Assis City, Brazil. METHODS: The study included adults who passed the due date for taking the COVID-19 second dose vaccine. Participants were recruited in December 2021 using a mobile-based text message. Sociodemographic and clinical data and reasons for hesitance were collected. The outcome was the attitude towards completing the recommended second dose of the vaccine. Bivariate and multivariate Poisson analyses were performed to determine the adjusted predictors. RESULTS: Participants between 30–44 years of age had a 2.41 times higher prevalence of hesitation than those aged 18–29 years. In addition, people who had adverse events or previously had COVID-19 had 4.7 and 5.4 times higher prevalences of hesitation, respectively (P value < 0.05). CONCLUSION: We found a significant group of adults aged between 30–44 years who refused the second dose of the COVID-19 vaccine. Furthermore, those who reported adverse effects after the first dose and those who had COVID-19 previously were a significant group for refusal.

https://doi.org/10.1590/1516-3180.2022.0095.r1.06072022

Identification of Heliothinae species (Lepidoptera: Noctuidae) in chickpeas in central Brazil
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Reyes, Caroline P

; Queiroz, Paulo Roberto M

; Specht, Alexandre

; Monnerat, Rose

Horticultura Brasileira

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2023, Volume 41 elocation e2534

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Chickpea is a commercially exploited crop on a relatively new scale in Brazil, that is why, little information on the main pests is available. Mostly caterpillars showing Heliothinae morphological characteristics have emerged in production areas. In order to point out the species of these insects in chickpea fields produced in the Federal District and the surrounding areas, this study aimed to determine the morphological and molecular identification of these invertebrates in order to assist producers in pest management in a more sustainable way. Caterpillars were hand-collected directly from the plants in six sites and the moth collection was collected using light traps in four sites, monthly, in 2017 and 2018. The morphological identification was made through the adult insect genitalia and the subsequent molecular identification was performed using the cytochrome oxidase I enzyme with the quantitative PCR technique, which resulted in the development of an efficient and low-cost DNA extraction protocol to be used in samples under unfavorable storage conditions. Morphological and molecular studies showed the presence of Helicoverpa armigera, Helicoverpa zea, Chloridea virescens and Chloridea subflexa, with a predominance of Chloridea virescens in chickpea plants. Chloridea subflexa was verified for the first time feeding on chickpeas in Brazil.

RESUMO O grão-de-bico é uma cultura com exploração comercial em escala relativamente nova no país e, por isso, há poucas informações disponíveis sobre as principais pragas, majoritariamente lagartas, com características morfológicas de Heliothinae, que têm surgido nas áreas de produção. Com o intuito de apontar as espécies desses insetos no grão-de-bico produzido no Distrito Federal e região produtora em seu entorno, este trabalho foi realizado para a identificação morfológica e molecular desses invertebrados para auxiliar os produtores no manejo de pragas de forma mais sustentável. Foram realizadas coletas manuais de lagartas diretamente nas plantas em seis locais e a coleta de mariposas com o uso de armadilhas luminosas em quatro locais, mensalmente, durante os anos de 2017 e 2018. A identificação morfológica foi feita através da genitália dos insetos adultos e a posterior identificação molecular foi realizada através da enzima citocromo oxidase I com a técnica de PCR quantitativo, o que resultou no desenvolvimento de um protocolo de extração de DNA eficiente e de baixo custo para o uso em amostras sob condições desfavoráveis de armazenamento. Os estudos morfológico e molecular resultaram na constatação da presença de Helicoverpa armigera, Helicoverpa zea, Chloridea virescens e Chloridea subflexa, com predominância de Chloridea virescens nas plantas de grão-de-bico. Foi registrada pela primeira vez Chloridea subflexa se alimentando em grão-de-bico no Brasil.

https://doi.org/10.1590/s0102-0536-2023-e2534

DOES PARTIAL MEDIAL KNEE ARTHROPLATIES HAVE BETTER RESULTS THAN TOTAL ONES?
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GUERREIRO, JOÃO PAULO FERNANDES

; SANTOS, VITORIA KAROLINE JUSTINO DOS

; MATZ, LUCAS BECKERT

; PEDROLLO, LUCAS DELFINO

; SANTOS, VITOR HENRIQUE JUSTINO DOS

; QUEIROZ, ALEXANDRE OLIVEIRA

; BIGNARDI, PAULO ROBERTO

; DANIELI, MARCUS VINICIUS

Acta Ortopédica Brasileira

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2023, Volume 31 N. spe2 elocation e262186

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective Compare the results of medial unicompartmental knee arthroplasty (UKA) using a mobile platform and total knee arthroplasty (TKA) in patients with isolated medial osteoarthritis. Methods Retrospectivecross-sectional study. Preoperative radiographs of 602 patientswho underwent knee arthroplastybetween February 2017 and February 2020 were evaluated. Isolated medial osteoarthritis was found in 125 patients. Of these, 57 underwent UKA and 68 TKA. With chart analysis and telephone interviews, we compared patients’ clinical outcomes and degree of satisfaction. The statistical analysis used a confidence level of 5%. Results The group of UKA patients obtained 65.8% of favorable results against 79.1% of those undergoing TKA in the function questionnaire (p<0.0001). The complication rate was statistically similar between the groups(p>0.5). Most patients were satisfied or very satisfied in both groups (88.6% of UKA and 91.2% of TKA) (p>0.999). Conclusion Patients submitted to UKA or TKA have presented the same degree of satisfaction and rate of postoperative complications when comparing patients with isolated medial osteoarthritis. UKA patients had less favorable results onthe clinical functional questionnaire than patients undergoing total arthroplasty. Level Of Evidence III;Retrospective Study.

RESUMO Objetivo Comparar os resultados da artroplastia unicompartimental do joelho (UKA) medial com plataforma móvel e artroplastia total do joelho (TKA) em pacientes com osteoartrose medial isolada. Métodos Estudo transversal retrospectivo. Foram avaliadas radiografias pré-operatórias de 602 pacientes submetidos à artroplastia de joelho entre fevereiro de 2017 e fevereiro de 2020. A osteoartrose medial isolada foi encontrada em 125 pacientes, destes em 57 haviam sido submetidos a UKA e 68 TKA. Com análise de prontuários e entrevistas telefônicas comparamos os resultados clínicos e o grau de satisfação dos pacientes. A análise estatística utilizou nível de confiança de 5%. Resultados O grupo de pacientes com UKA obteve 65,8% de resultados favoráveis contra 79,1% daqueles submetidos à TKA no questionário de função (p<0,0001). A taxa de complicações foi estatisticamente semelhante entre os grupos (p>0,5). A maioria dos pacientes estava satisfeita ou muito satisfeita em ambos os grupos (88,6% de UKA e 91,2% de TKA) (p>0,999). Conclusão Pacientes submetidos a UKA ou TKA apresentaram o mesmo grau de satisfação e taxa de complicações pós-operatórias quando comparados pacientes com osteoartrite medial isolada. Os pacientes da UKA apresentaram resultados menos favoráveis ao questionário clínico funcional do que os pacientes submetidos à artroplastia total. Nível de Evidência III; Estudo Retrospectivo.

https://doi.org/10.1590/1413-785220233102e262186

THE RESULTS OF A COMPARISON OF A BRAZILIAN AND AN IMPORTED KNEE PROSTHESIS - 5 YEARS OF FOLLOW-UP
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CALEGARI, JOAO HENRIQUE COSTA

; MARQUEZE, THIAGO CAVALCANTE COELHO

; KADRI, OMAR SHARID TEIXEIRA EL

; PEREIRA, EIKE JEFFERSON GALDINO

; QUEIROZ, ALEXANDRE OLIVEIRA

; BIGNARDI, PAULO ROBERTO

; DANIELI, MARCUS VINICIUS

; GUERREIRO, JOÃO PAULO FERNANDES

Acta Ortopédica Brasileira

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2022, Volume 30 N. 1 elocation e253870

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Introdução Comparar resultados funcionais, índices de satisfação e revisões de artroplastias totais de joelho realizadas pela mesma equipe cirúrgica usando implantes brasileiros ou importados, com acompanhamento mínimo de cinco anos após a cirurgia. Material e Método Estudo de coorte retrospectivo com análise de prontuários e entrevistas de pacientes submetidos à artroplastia total do joelho com implantes brasileiros e importados com no mínimo cinco anos de pós-operatório. Resultados Foram avaliados 150 pacientes (164 joelhos). No questionário funcional, encontramos 71% dos pacientes com respostas favoráveis no grupo de pacientes submetidos à cirurgia com uso de próteses brasileiras e 74,8% no grupo com implantes importados (p = 0,634). Em relação à satisfação, não houve diferença estatística entre os grupos com 78,4% dos pacientes satisfeitos ou muito satisfeitos no grupo com implante brasileiro e 90,7% no grupo com implante importado (p = 0,053). A ocorrência de soltura dos implantes foi relatada em 5,3% versus 4,7% (p > 0,999). Conclusões As artroplastias totais de joelho realizadas pela mesma equipe cirúrgica com acompanhamento mínimo de cinco anos apresentaram níveis semelhantes de satisfação, função e complicações com os implantes brasileiros e importados. Nível de evidência III, estudo de coorte.

ABSTRACT Introduction To compare the functional results, satisfaction rates, and revisions of total knee arthroplasties performed by the same surgical team using either Brazilian or imported implants, with a minimum follow-up of 5 years after surgery. Materials and Methods A retrospective cohort study analyzing the medical records and interviews of patients who underwent total knee arthroplasty with Brazilian or imported implants with a minimum of 5 years after surgery. Results One hundred and fifty patients were evaluated (164 knees). In the functional questionnaire, 71% of patients had favorable answers in the group of patients who underwent surgery using the Brazilian prosthesis and 74.8% in the group with imported implants (p=0.634). There was no statistical difference in satisfaction between the groups, with 78.4% of patients satisfied or very satisfied in the Brazilian implant group and 90.7% in the imported implant group (p=0.053). Loosening of the implants was reported in 5.3% versus 4.7% (p>0.999). Conclusion The total knee arthroplasties performed by the same surgical team with a minimum follow-up period of 5 years showed similar levels of satisfaction, function, and complications with both the Brazilian and imported implants. Level of Evidence III, cohort study.

https://doi.org/10.1590/1413-785220223001e253870

Pequena Ferrovia, grandes problemas: A E. F. Mate Laranjeira (1911-1959)
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Queiroz, Paulo Roberto Cimó

História (São Paulo)

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2022, Volume 41 elocation e2022008

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A E. F. Mate Laranjeira, com bitola de 60 cm e extensão máxima de 68 km, foi construída no oeste do estado brasileiro do Paraná, na segunda década do século XX, em caráter privado, pela grande empresa que explorava, nessa época, os bosques nativos de erva-mate (Ilex paraguayensis), situados no extremo sul do vizinho estado de Mato Grosso. A via destinava-se a contornar as Sete Quedas, que interrompiam a navegação do rio Paraná na altura do paralelo 24º Sul, e, assim, facilitar o transporte da erva em direção ao principal mercado consumidor: a Argentina. Ao conectar dois trechos navegáveis do rio Paraná, a ferrovia atraiu a atenção dos dirigentes do estado do Paraná e do Estado nacional brasileiro, para os quais ela poderia favorecer a ocupação e o aproveitamento de toda a região, até então considerada remota e selvagem. Neste artigo, busco analisar a EFML como integrante da categoria das ferrovias industriais, abordando a história de sua construção, suas condições técnicas de operação e também a disputa em torno de sua abertura ao tráfego público. As fontes incluem legislação, periódicos, relatórios governamentais, dados estatísticos e documentos da própria empresa, além de relatos de viajantes e outros trabalhos não acadêmicos. A principal conclusão é a de que, embora útil para os propósitos privados, a ferrovia, devido a suas limitações intrínsecas, não estava apta a desempenhar um papel público de grande relevância.

ABSTRACT The Mate Laranjeira Railway, with a 60 cm gauge and a maximum length of 68 km, was privately built in the west of the Brazilian State of Paraná, in the second decade of the 20th century, by the large company that explored, at that time, the native forests of Paraguayan tea (Ilex paraguayensis) located in the extreme south of the neighboring State of Mato Grosso. The route was designed to bypass the Seven Falls, which interrupted the Paraná River navigation nearby the 24th parallel South, and thus facilitate the transport of the tea towards its main consumer market: Argentina. By connecting two navigable stretches of the Paraná River, the railroad could facilitate the occupation and exploration of the entire region, which until then was considered remote and wild, and therefore attracted the attention of the administrators from the State of Paraná and the Brazilian national state. In this article I seek to analyze this railroad as a member of the industrial railways category, addressing its construction history, its technical operating conditions and also the dispute over its opening to public traffic. The sources include legislation, periodicals, government reports, statistical data and documents from the company itself, as well as reports from travellers and other non-academic works. The main conclusion is that, although useful for private purposes, the railway, due to its intrinsic limitations, was not able to play a public role of great relevance.

https://doi.org/10.1590/1980-4369e2022008

Ultrasonographic evaluation of cecal appendix diameter in pediatric population
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Queiroz, Marcos Roberto Gomes de

; Francisco Neto, Miguel José

; Rahal Junior, Antonio

; Jabour, Victor Arantes

; Andrade, Guilherme Neves Lourenço

; Silva, Paulo Savoia Dias da

; Garcia, Rodrigo Gobbo

; Pereira, Marcelo dos Santos

; Santos, Marina Ramos

; Luna, Pedro Andrade Alencar

; Sameshima, Yoshino Tamaki

; Gual, Fabiana

; Dutra, Marcelo Guimarães

; Germino, Beatriz Placca

; Alves, Isabella Ferreira

einstein (São Paulo)

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2022, Volume 20 elocation eAO6935

Abstract: En | Text: En | PDF: En

ABSTRACT Objective To stratify ultrasound samples in a pediatric population undergoing evaluation for acute appendicitis to examine the variability in cecal appendix diameter, in different age groups, and to determine whether there is a prevalent value for each age group. Methods A retrospective cross-sectional study with 196 children aged 0 to 15 years. Data were extracted from reports of ultrasound examinations carried out between 2008 and 2015. Children with sonographic diagnosis of appendicitis or other signs of periappendiceal inflammation were excluded. Results The evaluation of the anteroposterior measurement of the cecal appendix revealed a mean diameter of 4.14mm (standard deviation: 0.93mm; 95%CI: 3.86-4.14). Cecal appendix diameter did not differ significant between age groups. Conclusion Evaluation of the anteroposterior diameter of the cecal appendix in centimeters in a sample of 196 children aged 0 to15 years revealed a mean diameter of 4.14mm (standard deviation, 0.93mm. There were no significant differences in cecal appendix diameter following stratification by age. Results indicate a single value can be adopted for mean cecal appendix diameter in pediatric populations.

https://doi.org/10.31744/einstein_journal/2022ao6935

Prediction and reasons for COVID-19 second dose vaccine hesitation: a cross-sectional study in a municipality of Brazil
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Sartorão-Filho, Carlos Izaias

; Zoqui, Mariana Costa

; Duarte, Douglas Otomo

; Ribeiro, Edy Alyson

; Bisetto, Vinicius César Queiroz

; Cachoni, Lara Escobar Gavião

; Sartorão, Ana Luísa Varrone

; Terribile, Diogo Coutinho

; Mello, Beatriz Balsimelli de

; Sartorão-Neto, Carlos Izaias

; Mello, Roberto de

Sao Paulo Medical Journal

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2022, N. ahead

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/1516-3180.2022.0095.r1.06072022

10.

Selection of Bacillus thuringiensis strains toxic to Meloidogyne incognita
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Santos, Jônatas Barros dos

; Silva, Alberto do Nascimento

; Queiroz, Paulo Roberto Martins

; Eckstein, Barbara

; Monnerat, Rose Gomes

Pesquisa Agropecuária Tropical

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2022, Volume 52 elocation e73070

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A bactéria Bacillus thuringiensis tem demonstrado potencial no controle de diversas pragas agrícolas, dentre elas os nematoides. Objetivou-se padronizar e estabelecer uma metodologia de bioensaios de B. thuringiensis e Meloidogyne incognita com o uso de resorcinol, selecionar estirpes de B. thuringiensis tóxicas a M. incognita in vitro e identifcar molecularmente a presença do gene cry6 em estirpes de B. thuringiensis. Juvenis de segundo estágio foram submetidos a doses de resorcinol, verifcando-se que a concentração de 0,2 % não causou mortalidade e proporcionou a ingestão da toxina Cry6A. Após isso, 16 estirpes de B. thuringiensis foram testadas na presença ou não de resorcinol, resultando em mortalidades de 4-36 %. Dentre as estirpes de B. thuringiensis analisadas quanto à presença do gene cry6, detectou-se apenas uma (S1617).

ABSTRACT The Bacillus thuringiensis bacterium has demonstrated an effective potential in the control of several agricultural pests, among them nematodes. This study aimed to standardize and establish a methodology of bioassays of B. thuringiensis and Meloidogyne incognita with the use of resorcinol, select B. thuringiensis strains toxic to M. incognita in vitro and molecularly identify the presence of the cry6 gene in B. thuringiensis strains. Second-stage juveniles were subjected to resorcinol doses, verifying that the concentration of 0.2 % did not cause mortality and provided the Cry6A toxin ingestion. Thereafter, 16 B. thuringiensis strains were tested in the presence or absence of resorcinol, resulting in mortality rates of 4-36 %. Among the B. thuringiensis strains analyzed for the presence of the cry6 gene, only one was detected (S1617).

https://doi.org/10.1590/1983-40632022v5273070

11.

The macroscopic and microscopic description of ruminal lesions in feedlot bovine
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Viana, Paulo Roberto Lucas

; Viana, Letícia Fleury

; Araújo, Gustavo Henrique Marques

; Moraes, Isadora David Tavares de

; Queiroz, Paulo José Bastos

; Cagnini, Didier Quevedo

; Silva, Luiz Antônio Franco da

; Rabelo, Rogério Elias

Ciência Animal Brasileira

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2022, Volume 23 elocation e-73109

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O uso de dietas ricas em carboidratos altamente fermentáveis e com baixos teores de fibras pode ocasionar desequilíbrio entre os microrganismos do rúmen com consequente acidose ruminal. Esse problema pode resultar em lesões na parede do rúmen, evoluindo com frequência para formação de ruminite. O presente estudo objetivou caracterizar lesões ruminais macroscópicas e microscópicas, observadas em bovinos confinados que apresentavam lesões podais ou abscesso hepático. Avaliou-se 1060 bovinos pelo exame post mortem. Foram encontradas lesões podais em 88 animais, abscesso hepático em 10 e lesões ruminais macroscópicas em 230 bovinos, sendo caracterizado 178 rumens com hiperqueratose, 41 com hiperemia, 9 com úlcera e 2 com neoplasia. Os 98 bovinos com lesões podais e abscesso hepático foram selecionados para realização de exame histopatológico. Desses, foram encontradas lesões macroscópicas em 23 e, microscópicas, em 23 animais. Dos 23 animais que apresentaram lesões macroscópicas, 10 tiveram as mesmas alterações verificadas na microscopia. Foram diagnosticados 7 casos de hiperqueratose na avaliação macro e microscópica. Dos 5 casos de hiperemia verificados na macroscopia, 2 casos foram identificados na microscopia e um caso de úlcera identificado na macroscopia também foi identificado na microscopia. A avaliação microscópica dos rumens permitiu identificar lesões em animais com lesões podais que não apresentam alterações macroscópicas ruminais.

Abstract Administration of diets rich in highly fermentable carbohydrates and low fiber content can cause an imbalance between the microorganisms in the rumen with consequent ruminal acidosis. This problem can cause lesions in the rumen wall, often progressing to rumenitis. The purpose of the present was to characterize macroscopic and microscopic ruminal lesions observed in confined feedlot cattle with claw lesions or liver abscess. A total of 1060 bovines were evaluated via postmortem examination. Claw lesions were identified in 88, liver abscess in 10, and macroscopic rumen lesions in 230 bovines; furthermore, 178 rumens were characterized with hyperkeratosis, 41 with hyperemia, 9 with ulcer, and 2 with neoplasia. The 98 bovines with claw lesions and liver abscess were selected for histopathological examination. Of these, macroscopic lesions were noted in 23 and microscopic lesions in 23 animals. Of the 23 animals that presented macroscopic lesions, 10 showed the same changes observed under microscopy. Seven cases of hyperkeratosis were diagnosed in the macro and microscopic evaluation. Of the 5 cases of hyperemia verified on macroscopy, 2 cases were identified via microscopy, and 1 case of ulcer identified through macroscopy and microscopy. The microscopic evaluation of the rumens allowed the identification of lesions in animals with claw lesions that did not present macroscopic rumen alterations.

https://doi.org/10.1590/1809-6891v23e-73109e

12.

Acoustic radiation force impulse elastography and liver fibrosis risk scores in severe obesity
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Silva Junior, Roberto Gomes da

; Miranda, Maria Luiza Queiroz de

; Brant, Paulo Eugênio de Araújo Caldeira

; Schulz, Perla Oliveira

; Nascimento, Maria de Fátima Araujo

; Schmillevitch, Joel

; Vieira, Andrea

; Freitas Junior, Wilson Rodrigues de

; Szutan, Luiz Arnaldo

Archives of Endocrinology and Metabolism

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dec 2021, Volume 65 N. 6 Pages 730 - 738

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: Identifying significant fibrosis is crucial to evaluate the prognosis and therapeutic interventions in patients with nonalcoholic fatty liver disease (NAFLD). We assessed the performance of acoustic radiation force impulse (ARFI) elastography, APRI, FIB-4, Forns, NFS and BARD scores in determining liver fibrosis in severe obesity. Subjects and methods: A prospective study included 108 patients undergoing bariatric surgery. Liver biopsy specimens were obtained intraoperatively and classified according to the NAFLD Activity Score. Patients were assessed with serological markers and shear wave velocity of the liver was measured with the Siemens S2000 ultrasound system preoperatively. Optimal cut-off values were determined using the area under the receiver operating characteristic curves (AUROC). Results: In the entire cohort prevalence of NAFLD was 80.6%, steatohepatitis 25.9% and significant fibrosis 19.4%. The best tests for predicting significant fibrosis were FIB-4 and Forns scores (both AUROC 0.78), followed by APRI (AUROC 0.74), NFS (AUROC 0.68), BARD (AUROC 0.64) and ARFI (AUROC 0.62). ARFI elastography was successful in 73% of the patients. Higher body mass index (BMI) correlated with invalid ARFI measurements. In patients with BMI < 42 kg/m2, ARFI showed 92.3% sensitivity and 82,6% specificity for the presence of significant fibrosis, with AUROC 0.86 and cut-off 1.32 m/s. Conclusions: FIB-4 and Forns scores were the most accurate for the prediction of significant fibrosis in bariatric patients. Applicability and accuracy of ARFI was limited in individuals with severe obesity. In patients with BMI < 42 kg/m2, ARFI elastography was capable for predicting significant fibrosis with relevant accuracy.

https://doi.org/10.20945/2359-3997000000397 2 downloads

13.

Influence of pupillary dynamics on the defocus curve of eyes implanted with diffractive multifocal lenses: a randomized study
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Messias, André

; Ferreira, Miriam

; Mendonça, Gleilton Carlos

; Queiroz, Wilian

; Coelho, Roberto Pinto

; Gekeler, Katrin

Arquivos Brasileiros de Oftalmologia

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jun 2021, Volume 84 N. 3 Pages 214 - 219

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Avaliar a influência da dinâmica pupilar na curva de desfoco de olhos implantados com lente intraoculares multifocais difrativas. Métodos: Estudo prospectivo e randomizado realizado na Faculdade de Medicina de Ribeirão Preto - Universidade de São Paulo - Departamento de Oftalmologia. Trinta e oito pacientes foram aleatoriamente designados para receber bilateralmente lentes intraoculares SN6AD1 (n=20) (mfIOL) ou SN60WF (n=18) (aIOL). Além da acuidade visual para longe e perto, corrigida e não corrigida, e curva de desfoco, foi ainda realizada pupilometria dinâmica. A área sob a curva de desfoco foi calculada usando um modelo polinomial empírico. Resultados: Um total de 16 e 17 pacientes (n=32 e 34 olhos) completaram 1 ano de seguimento nos grupos mfIOL e aIOL, respectivamente. Não houve diferenças significativas entre grupos para as acuidades visuais seja para longe ou perto. As curvas de desfoco do grupo mfIOL mostraram um pico duplo; enquanto o SN60WF mostrou apenas um pico, típico para uma lente intraoculares monofocal. A média da área sob a curva de desfoco do grupo aIOL foi (4,66 ± 1,51 logMAR.dp), e essa é estatisticamente significante diferente da métrica do grupo mfIOL (1,99 ± 1,31 logMAR.dp). A pupila na contração máxima após a exposição a um flash de 30 cd/m2 por 1 segundo foi significativamente correlacionada com uma melhor área de foco no grupo mfIOL (r=0,54; p=0,0017), essa relação não foi observada para o grupo aIOL. Conclusão: Estes dados indicam que quanto menor a pupila durante contração, melhor é a área sob a curva de desfoco e, portanto, o desempenho visual dos olhos implantados com essa mfIOL. Esta correlação não foi encontrada para lentes intraoculares monofocais.

ABSTRACT Purpose: To evaluate the influence of pupil dynamics on the defocus profile and area-of-focus of eyes implanted with a diffractive multifocal intraocular lens (IOL). Methods: This prospective randomized trial was conducted at the Department of Ophthalmology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil. Thirty-eight patients were randomly assigned to receive the multifocal SN6AD1 (n=20) or the aspheric monofocal SN60WF (aIOL) (n=18) IOLs bilaterally. Dynamic pupillometry, visual acuity for distance and near, corrected and uncorrected, and a defocus profile were assessed postoperatively. The area-of-focus was calculated using an empirical polynomial model of the defocus profile. Results: Sixteen patients (32 eyes) in the multifocal SN6AD1 group and 17 patients (34 eyes) in the aspheric monofocal SN60WF group completed the 1-year follow-up. There were no significant between-group differences in monocular uncorrected distance or near visual acuity. The defocus profiles of the mfIOL group showed a double peak, whereas those of the aspheric monofocal SN60WF group showed only one peak, which is typical for a monofocal intraocular lens. The area-of-focus of the aIOL group (4.66 ± 1.51 logMARxD) was significantly different from that of the multifocal SN6AD1 (1.99 ± 1.31 logMARxD). Pupil size at maximum contraction after exposure to a flash of 30 cd/m2 for 1 second was significantly correlated with a better area-of-focus in the multifocal SN6AD1 group (r=0.54; p=0.0017), whereas this was not the case in the aspheric monofocal SN60WF group. Conclusion: These findings indicate that in eyes implanted with an multifocal SN6AD1, the smaller the pupil size, the better is the area-of-focus and hence the better is the visual performance. This correlation was not found for the aspheric monofocal SN60WF.

https://doi.org/10.5935/0004-2749.20210029 148 downloads

14.

Implications for Clinical Practice from a Multicenter Survey of Heart Failure Management Centers
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Bocchi, Edimar Alcides

; Moreira, Henrique Turin

; Nakamuta, Juliana Sanajotti

; Simões, Marcus Vinicius

; Casas, Alberto de Almeida Las

; Costa, Altamiro Reis da

; Assis, Amberson Vieira de

; Durães, André Rodrigues

; Pereira-Barretto, Antonio Carlos

; Ravessa, Antonio Delduque de Araujo

; Macedo, Ariane Vieira Scarlatelli

; Biselli, Bruno

; Pinto, Carolina Maria Nogueira

; Filho, Conrado Roberto Hoffmann

; Costantini, Costantino Roberto

; Almeida, Dirceu Rodrigues

; Santos Jr, Edval Gomes dos

; Soliva Junior, Erwin

; Figueiredo, Estevão Lanna

; Albuquerque, Felipe Neves de

; Paulitsch, Felipe

; Neuenschwander, Fernando Carvalho

; Figueiredo Neto, José Albuquerque de

; Brito, Flavio de Souza

; Lopes, Heno Ferreira

; Villacorta, Humberto

; Souza Neto, João David de

; Sepulveda, João Mariano

; Ayoub, José Carlos Aidar

; Vilela-Martin, José F.

; Cardoso, Juliano Novaes

; Uemura, Laercio

; Moura, Lidia Zytynski

; Maia, Lilia Nigro

; Oliveira, Lucia Brandão de

; Maia, Lucimir

; Silva, Luís Beck da

; Gowdak, Luís Henrique Wolff

; Danzmann, Luiz Claudio

; Andrade, Marcus

; Braile-Sternieri, Maria Christiane Valeria Braga

; Moreira, Maria da Consolação Vieira

; França Neto, Olimpio R

; Filho, Otavio Rizzi Coelho

; Esteves, Paulo Frederico

; Raupp-da-Rosa, Priscila

; Silva, Ricardo Jorge de Queiroz e

; Mourilhe-Rocha, Ricardo

; Viégas, Ruy Felipe Melo

; Rassi, Salvador

; Mangili, Sandrigo

; Kaiser, Sergio Emanuel

; Martins, Silvia Marinho

; Kawabata, Vitor Sergio

Clinics

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2021, Volume 76 elocation e1991

Abstract: En | Text: En | PDF: En

OBJECTIVES: This observational, cross-sectional study based aimed to test whether heart failure (HF)-disease management program (DMP) components are influencing care and clinical decision-making in Brazil. METHODS: The survey respondents were cardiologists recommended by experts in the field and invited to participate in the survey via printed form or email. The survey consisted of 29 questions addressing site demographics, public versus private infrastructure, HF baseline data of patients, clinical management of HF, performance indicators, and perceptions about HF treatment. RESULTS: Data were obtained from 98 centers (58% public and 42% private practice) distributed across Brazil. Public HF-DMPs compared to private HF-DMP were associated with a higher percentage of HF-DMP-dedicated services (79% vs 24%; OR: 12, 95% CI: 94-34), multidisciplinary HF (MHF)-DMP [84% vs 65%; OR: 3; 95% CI: 1-8), HF educational programs (49% vs 18%; OR: 4; 95% CI: 1-2), written instructions before hospital discharge (83% vs 76%; OR: 1; 95% CI: 0-5), rehabilitation (69% vs 39%; OR: 3; 95% CI: 1-9), monitoring (44% vs 29%; OR: 2; 95% CI: 1-5), guideline-directed medical therapy-HF use (94% vs 85%; OR: 3; 95% CI: 0-15), and less B-type natriuretic peptide (BNP) dosage (73% vs 88%; OR: 3; 95% CI: 1-9), and key performance indicators (37% vs 60%; OR: 3; 95% CI: 1-7). In comparison to non- MHF-DMP, MHF-DMP was associated with more educational initiatives (42% vs 6%; OR: 12; 95% CI: 1-97), written instructions (83% vs 68%; OR: 2: 95% CI: 1-7), rehabilitation (69% vs 17%; OR: 11; 95% CI: 3-44), monitoring (47% vs 6%; OR: 14; 95% CI: 2-115), GDMT-HF (92% vs 83%; OR: 3; 95% CI: 0-15). In addition, there were less use of BNP as a biomarker (70% vs 84%; OR: 2; 95% CI: 1-8) and key performance indicators (35% vs 51%; OR: 2; 95% CI: 91,6) in the non-MHF group. Physicians considered changing or introducing new medications mostly when patients were hospitalized or when observing worsening disease and/or symptoms. Adherence to drug treatment and non-drug treatment factors were the greatest medical problems associated with HF treatment. CONCLUSION: HF-DMPs are highly heterogeneous. New strategies for HF care should consider the present study highlights and clinical decision-making processes to improve HF patient care.

https://doi.org/10.6061/clinics/2021/e1991 870 downloads

15.

Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?
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Batista, Thiago Barbosa

; Perissato, Samara Moreira

; Rego, Carlos Henrique Queiroz

; Oliveira, Gustavo Roberto Fonseca de

; Henning, Fernando Augusto

; Silva, Edvaldo Aparecido Amaral da

Journal of Seed Science

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2021, Volume 43 elocation e202143024

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo: A análise da longevidade de um lote de sementes pode auxiliar na decisão de por quanto tempo armazenar um lote de sementes até a sua comercialização, uma vez que esta característica implica na manutenção da viabilidade ao longo do tempo. A longevidade é analisada pelo teste de p50, o qual representa o tempo para perda de 50% da viabilidade inicial. Sementes com elevada germinação e vigor têm maior potencial fisiológico e, assim, maior capacidade de manter a qualidade ao longo do período de armazenamento. No entanto, pouco tem sido estudado quanto às correlações entre a análise do p50 (longevidade) com os testes utilizados para mensurar o potencial fisiológico de lotes (germinação e vigor), o que pode ser utilizado como ferramenta para obter inferências sobre a longevidade por meio dos testes mais comumente utilizados. Assim, objetivou-se investigar quais testes utilizados para mensurar o potencial fisiológico de lotes pode ser utilizado para estimar o p50. Para isso, foram avaliados a germinação e o vigor, utilizando-se testes tradicionais e, em paralelo, a longevidade em oito lotes de sementes de soja. As correlações e a regressão linear para as variáveis tradicionais versus p50 também foram testadas. Foi verificado que o uso dos testes de envelhecimento acelerado, condutividade elétrica e tempo para 50% de protrusão da radícula tem elevado potencial para estimar a longevidade mensurada pelo p50.

Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.

https://doi.org/10.1590/2317-1545v43249697 132 downloads

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