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ABSTRACT The expression ‘you need to know to conserve’ is a well-known cliche among biologists. Documenting the richness of a group of organisms is the first step towards understanding biodiversity and preparing efficient conservation plans. In this context, many efforts have been made to quantify the number of species on Earth and estimate the number of species still unknown to science. A few countries have complete and integrated databases estimating the approximate number of species recorded for their territory, particularly in the Global South. In Brazil, a country of continental dimensions, revealing the richness of the second most diverse clade of invertebrates (=Mollusca) has been a goal of taxonomists. Recently, in an unprecedented, collective, and integrated effort among Brazilian malacologists, it was possible to estimate how many valid species of molluscs are there in Brazil. In this effort, more than 30 mollusc experts joined together to update the Taxonomic Catalogue of the Brazilian Fauna (TCBF), a governmental website that allows a quick and real-time updating of all Metazoan. So far, more than 5,000 updates have been made in TCBF, indicating the presence of 3,552 valid species of molluscs in Brazil, distributed among the main clades as follows: Caudofoveata (10 spp.), Solenogastres (6 spp.), Polyplacophora (35 spp.), Scaphopoda (43 spp.), Cephalopoda (92 spp.), Bivalvia (629 spp.) and Gastropoda (2,737 spp.). The present study, in addition to demonstrating for the first time the richness of Brazilian molluscs, also presents the state of the art of this important phylum of invertebrates highlighting its most representative and neglected groups.

https://doi.org/10.1590/s1984-4689.v40.e23026

Diretriz da Sociedade Brasileira de Cardiologia sobre a Análise e Emissão de Laudos Eletrocardiográficos – 2022
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Samesima, Nelson

; God, Epotamenides Good

; Kruse, Jose Claudio Lupi

; Leal, Marcelo Garcia

; Pinho, Claudio

; França, Francisco Faustino de A. C.

; Pimenta, João

; Cardoso, Acácio Fernandes

; Paixão, Adail

; Fonseca, Alfredo

; Pérez-Riera, Andrés R.

; Ribeiro, Antonio Luiz Pinho

; Madaloso, Bruna Affonso

; Luna Filho, Bráulio

; Oliveira, Carlos Alberto Rodrigues de

; Grupi, César José

; Moreira, Dalmo Antonio Ribeiro

; Kaiser, Elisabeth

; Paixão, Gabriela Miana de Mattos

; Feitosa Filho, Gilson

; Pereira Filho, Horacio Gomes

; Grindler, José

; Aziz, José Luiz

; Molina, Marcos Sleiman

; Facin, Mirella

; Tobias, Nancy M. M. de Oliveira

; Oliveira, Patricia Alves de

; Sanches, Paulo César R.

; Teixeira, Ricardo Alkmin

; Atanes, Severiano Melo

; Pastore, Carlos Alberto

Arquivos Brasileiros de Cardiologia

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oct 2022, Volume 119 N. 4 Pages 638 - 680

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20220623

Aspergillus niger: A Hundred Years of Contribution to the Natural Products Chemistry
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Lima, Mary Anne S.

; Oliveira, Maria da Conceição F. de

; Pimenta, Antônia T. Á.

; Uchôa, Paula K. S.

Journal of the Brazilian Chemical Society

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oct 2019, Volume 30 N. 10 Pages 2029 - 2059

Abstract: En | Text: En | PDF: En

Aspergillus niger is a phytopathogenic fungus responsible for the plant disease called “black mold”, and it is considered the most versatile microorganism for producing acids, proteins, and enzymes of industrial value, besides a variety of compounds of pharmacological interest. This review presents a century of contribution of A. niger in the natural products chemistry under two different perspectives: (i) an overview of the structural diversity of secondary metabolites produced by A. niger from different habitats and their biological activities; (ii) a general discussion of the enzymatic potential of A. niger on the selective biotransformation of terpenes, highlighting the most uncommon microbial transformations.

https://doi.org/10.21577/0103-5053.20190080 2452 downloads

New flavone and other compounds from Tephrosia egregia: assessing the cytotoxic effect on human tumor cell lines
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Teixeira, Maria V.S.

; Lima, Jefferson Q.

; Pimenta, Antônia T.A.

; Silva, Francisca R.L. da

; Oliveira, Maria da Conceição F. de

; Pereira, Italo G.

; Costa-Junior, Dilailson C.

; Ribeiro, Paulo R.V.

; Santiago, Gilvandete M.P.

; Lima, Mary Anne S.

; Braz-Filho, Raimundo

; Bezerra, Mirna M.

; Montenegro, Raquel C.

; Rocha, Danilo D.

; Moraes, Maria Elisabete A. de

; Medeiros, Aline C. de

; Arriaga, Angela M.C.

Revista Brasileira de Farmacognosia

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jun 2018, Volume 28 N. 3 Pages 333 - 338

Abstract: En | Text: En | PDF: En

ABSTRACT The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided a new flavone, 5-hydroxy-8-(1",2"-epoxy-3"-hydroxy-3"-methylbutyl)-7-methoxyflavone (1), in addition to eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecansone A, 2',6'-dimethoxy-4',5'-(2",2"-dimethyl)-pyranochalcone, pongachalcone, maackiain, β-sistosterol and its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving comparison with literature data. Cytotoxicity of compounds 1–4 was evaluated against AGP-01 (cancerous ascitic fluid), HCT-116 (colon adenocarcinoma), HL-60 (leukemia), PC-3 (prostate carcinoma), SF-295 (glioblastoma) and SKMEL 28 (melanoma) cell lines.

https://doi.org/10.1016/j.bjp.2018.03.008 525 downloads

DLLME-GC/ECD Method for the Residual Analysis of Parathion-Methyl and its Application in the Study of the UV-Photodegradation Process
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Pimenta, Gustavo G.

; Queiroz, Maria E. L. R. de

; Victor, Raquel P. D.

; Noronha, Luiz M.

; Neves, Antônio A.

; Oliveira, André F. de

; Heleno, Fernanda F.

Journal of the Brazilian Chemical Society

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nov 2017, Volume 28 N. 11 Pages 2045 - 2053

Abstract: En | Text: En | PDF: En

A new method has been developed to determine trace levels of organophosphorus pesticide parathion-methyl in water samples by using dispersive liquid-liquid microextraction (DLLME), followed by gas chromatograph coupled with electron capture detector (GC/ECD) analysis. The optimized and validated method showed high extraction recovery (101.1%), high enrichment factor (57.3), low limits of detection and quantification, 0.083 and 0.250 µg L-1, respectively. The established DLLME-GC/ECD method has been successfully applied for the evaluation of the photodegradation of the parathion-methyl by UV254nm radiation in different conditions of pH and temperature. The parathion-methyl photodegradation at pH 3 and 35 °C achieved > 99.5% after 120 min of exposition. For this condition, it was observed the kinetic rate of 0.0515 min-1, the quantum yield of 1.22 × 10-5 mol Einstein-1 and a half-life time of 13.46 min. All experimental conditions tested proved to be strongly influenced by pH and temperature. The application of the optimized process in distilled and drinking water spiked with parathion-methyl provided residues of this pesticide at levels below the maximum permitted by Brazilian legislation, which is 9.0 µg L-1.

https://doi.org/10.21577/0103-5053.20170048 683 downloads

Utilização de filme de quitosana para o controle de aflatoxinas em amendoim
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Silva, Juliana Fonseca Moreira da

; Prado, Guilherme

; Madeira, Jovita Eugênia Gazzinelli Cruz

; Oliveira, Marize Silva

; Faraco, André Augusto Gomes

; Malta, Camilla Martins

; Nicoli, Jacques Robert

; Pimenta, Raphael Sanzio

Bragantia

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dec 2015, Volume 74 N. 4 Pages 467 - 475

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste estudo, verificou-se a utilização de um filme de quitosana para o controle do crescimento e produção de aflatoxinas por A. parasiticus em amendoim. Os filmes foram aplicados sobre os grãos por meio de duas metodologias (aspersão e imersão). Os grãos recobertos foram inoculados com 2,5 ml de uma suspensão contendo 1,0×106 esporos/ml e incubados a 25 oC por 7 dias. A concentração de aflatoxinas foi determinada por cromatografia em camada delgada, utilizando a técnica de densitometria. A verificação da inibição do crescimento fúngico foi realizada por meio da inoculação do patógeno em placas de Petri contendo GYEP suplementado com quitosana a 2% e incubadas por 7 dias a 25 ºC. Após incubação, o diâmetro da colônia do patógeno foi estimado e comparado com o controle. Todos os testes foram realizados com cinco repetições. Para a verificação de alterações morfológicas, uma suspensão de esporos ou hifas incubados na presença de quitosana foi submetida à microscopia eletrônica de varredura. Foi observado que tanto o método de imersão quanto o de aspersão reduziram a produção de aflatoxina significativamente, 84,3% e 86,7% respectivamente. A presença de quitosana no meio de cultura reduziu o diâmetro das colônias e promoveu modificações morfológicas nos esporos de A. parasiticus. Com esse procedimento foi verificado que a utilização de quitosana a 2% na forma de filme, principalmente por aspersão, pode reduzir a concentração de esporos e aflatoxinas de A. parasiticus em amendoim, o que confere a essa substância uma grande perspectiva de utilização no controle de fungos aflatoxigênicos.

In this study, we evaluate the utilization on chitosan film to control the growth and aflatoxin production by A. parasiticus in peanut. The films were inoculated over the grains by aspersion or immersion methods. The chitosan coated grains were inoculated with 2.5 ml solution with 1.0×106spores/ml and incubated at 25 °C for 7 days. The aflatoxin concentration was estimated by thin layer chromatography and densitometry. The fungus growth inhibition was observed by pathogen inoculation in Petri dishes with GYEP media plus 2% chitosan and incubated by 7 days at 25 °C. After incubation the colony diameter was measured and compared with the control. All assays were repeated five times. To verify morphological alterations, a spore/hypha suspension was incubated in media with chitosan and observed in scanning electron microscopy.Both methods (immersion and aspersion) were able to reduce the aflatoxin production significantly, 84.3% and 86.7%, respectively. The chitosan reduced the colonies diameters and promoted morphological alterations in A. parasiticus spores. With this, was observed that a 2% chitosan film, specially inoculated by aspersion, can to reduce A. parasiticus spores and aflatoxins concentrations in peanuts. This characteristic gives to this substance an excellent perspective to be used in aflatoxigenic fungi control.

https://doi.org/10.1590/1678-4499.0120 2934 downloads

FRUIT MORPHOLOGY AND PRODUCTIVITY OF BABASSU PALMS IN NORTHERN MINAS GERAIS STATE, BRAZIL1
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Guedes, Marcio Lisboa

; Ferreira, Paulo Henrique Gonçalves

; Santana, Karla Nayara Oliveira

; Pimenta, Márcio Antônio Silva

; Ribeiro, Leonardo Monteiro

Revista Árvore

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oct 2015, Volume 39 N. 5 Pages 883 - 892

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Os objetivos deste trabalho foram caracterizar morfologicamente os frutos de babaçu e estimar a sua produtividade (Attalea vitrivir) no Norte de Minas Gerais. Foram coletados 20 frutos maduros de 10 plantas em três áreas, no Município de Januária, MG Mensuraram-se 18 parâmetros biométricos dos frutos, determinou-se o teor de óleo das sementes e avaliaram-se a aderência à distribuição normal, a distribuição de frequência e a correlação entre as variáveis e o efeito do indivíduo e da área. Quantificou-se a produção de cachos por planta e de frutos por cacho em 10 plantas, nas três áreas, e estimou-se a produtividade potencial em condição de extrativismo e de cultivo. Em todas as variáveis biométricas estudadas, houve diferença significativa entre os indivíduos e áreas, evidenciando-se grande variabilidade na morfologia dos frutos. O teor médio de óleo foi de 45, 7%, com diferença significativa entre os indivíduos. A grande variabilidade encontradafavorece, em programas de melhoramento, a seleção de indivíduos mais produtivos. A produtividade potencial de endocarpo e óleo em densidade de 400 plantas/ha é de 6,4 e 1,2 t/ha, respectivamente, o que amplia a possibilidade de uso de A. vitrivir na produção de carvão, na indústria de biocombustíveis e na fixação de carbono.

ABSTRACT The objectives of this study were to morphologically characterize fruits of the babassu palm tree (Attalea vitrivir) and to estimate their productivity in the north of Minas Gerais State, Brazil. Twenty mature fruits were collected from 10 plants in three different areas in Januária, Minas Gerais. Eighteen biometric parameters of the fruits were measured, the oil contents of the seeds was determined, the adherence to normal distribution was evaluated, distribution frequencies were evaluated and the effects of individuals and areas on the variables and the correlations between them were analyzed. The production of fruit bunches per plant and the number of fruits per bunch from 10 plants were quantified in three areas and the potential production under both natural harvesting and cultivation conditions were estimated. Significant differences were found among all of the biometric parameters examined between the different individuals and the different areas, which shows wide morphological variability in the fruits. The average oil content was 45.7%, but with significant differences among individuals. The observed variability favors the selection of productive individuals in genetic improvement programs. The potential productivity of endocarps and oil based on a density of 400/plants per hectare would be respectively 6.4 and 1.2 tons/ha, which indicates the possibility of using A. vitrivir for producing charcoal, bio fuels, and for carbon fixation.

https://doi.org/10.1590/0100-67622015000500011 1762 downloads

Avaliação físico-química e energética da madeira das espécies Piptadenia stipulacea (Benth.) Ducke e Amburana cearensis (Allemao) A. C. Smith de ocorrência no semiárido nordestino brasileiro
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Almeida, Antonio Marcos César de

; Oliveira, Elisabeth de

; Calegari, Leandro

; Medeiros Neto, Pedro Nicó de

; Pimenta, Alexandre Santos

Ciência Florestal

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mar 2015, Volume 25 N. 1 Pages 165 - 173

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A Caatinga é um bioma exclusivamente brasileiro, que apresenta uma grande variedade de espécies da fauna e da flora, sendo uma boa parte destas endêmicas. Ao longo dos anos, a supressão da caatinga por ação antrópica vem reduzindo bastante esse bioma. Este fato se dá, principalmente, pelo uso do potencial energético de suas espécies vegetais sem a devida preocupação com a sustentabilidade do mesmo. Um melhor conhecimento do potencial energético das espécies vegetais da caatinga possibilita um manejo mais adequado. O objetivo deste trabalho foi avaliar o potencial energético e as características físico-químicas da madeira das espécies Amburana cearensis (Allemao) A. C. Smithe Piptadenia stipulacea (Benth.) Ducke. Foram abatidas cinco árvores de cada espécie, amostradas aleatoriamente no sítio São Bento, localizado no Município de Patos - PB. As árvores foram devidamente identificadas e transportadas para o Setor de Tecnologia de Produtos Florestais (STPF) da Universidade Federal de Campina Grande. De cada árvore, foram retirados discos pequenos (5 cm de espessura) a 0 (base), 25, 50, 75 e 100% da altura comercial do tronco (até 5 cm de diâmetro) e discos grandes (15 cm espessura), antes e após cada disco pequeno, os discos pequenos foram subdividido em quatro partes, em forma de cunha, passando pela medula. Duas cunhas opostas foram utilizadas para a determinação da densidade básica, e as restantes foram reservadas para a realização das análises físicas e químicas da madeira e os discos maiores foram destinados à realização das carbonizações. A densidade básica foi determinada de acordo com o método da balança hidrostática e para a determinação da densidade básica média de cada árvore utilizou-se como fator de ponderação o volume entre seções de cada disco. Após a secagem ao ar, as amostras destinadas às análises químicas foram transformadas em serragem e feitas determinações quantitativas de extrativos total, da lignina, das cinzas, e o teor de holocelulose foi estimado por diferença em relação à massa inicial [100% - (teor de extrativos totais + teor de lignina + teor de cinzas)]. As amostras destinadas às carbonizações foram transformadas em cavacos, e carbonizadas em forno elétrico (mufla) por 5h30min. Foram feitas determinações dos rendimentos dos produtos das carbonizações, e das propriedades físicas e químicas do carvão vegetal. Médias significativamente superiores a 1% de probabilidade de teor de lignina, teor de holocelulose, extrativos totais e de cinzas da madeira foram encontradas para as espécies Amburana cearensis Piptadenia stipulacea, sendo que esta última espécie apresentou maiores médias de teor de lignina e teor de holocelulose. O rendimento gravimétrico foi semelhante para ambas as espécies, enquanto o rendimento em líquido condensado foi significativamente superior para a Amburana cearensis. Em relação à qualidade do carvão, foram observados maiores valores de poder calorífico superior e carbono fixo para a Piptadenia stipulacea, enquanto a Amburana cearensis exibiu maiores teores de materiais voláteis e de cinzas.

'Caatinga' is an exclusive Brazilian biome, which features a wide variety of fauna and flora, and a good part of these are endemic varieties. Over the years, the abolition of 'Caatinga' by human activities has greatly reduced this biome. This fact is mainly due to the use of the energy potential of plant species without proper concern about the sustainability of it. A better understanding of the energy potential of plant species of 'Caatinga' enables a more appropriate management. The objective of this study was to evaluate the potential energy and the physical and chemical characteristics of wood species Amburana cearensis (Germans) A. C. Smith and Piptadenia stipulacea (Benth.) Ducke. Five trees of each species were felled, randomly sampled in 'São Bento' site, located in the city of Patos, Paraíba state. The trees were identified and transported to the Department of Forest Product Technology, Federal University of Campina Grande. From each tree, small discs were removed (5 cm thick) to 0 (base), 25, 50, 75 and 100% of the commercial height of the trunk (up to 5 cm in diameter) and large disks (15 cm thick) before and after each small disk, the small discs were divided into four parts, wedge-shaped, passing through the medulla. Two opposing wedges were used to determine the density and the rest was reserved for the physical and chemical wood analyses and the wood discs were destined for greater achievements of carbonizations. Basic density was determined according to the method of hydrostatic balance and to determine the basic density of each tree used as a weighting factor the volume between sections of each disk. After air drying, the samples for chemical analysis were transformed into sawdust and made quantitative determinations of total extractives, lignin, ash and holocellulose content was estimated by difference from the initial mass [100% - (content total extractives + lignin + ash)]. Samples intended for carbonizations were transformed into chips, and charred in an electric furnace (furnace) for 5 hours and 30 minutes. The income from the carbonization products as well as the physical and chemical properties of charcoal were determined. Averages significantly higher than 1% probability of lignin, holocellulose content, total extractives and ash wood were found for the species Amburana cearensis and Piptadenia stipulacea, and this latter had higher average lignin content and holocellulose content. The gravimetric yield was similar for both species, while the condensed liquid yield was significantly higher for Amburana cearensis. In relation to coal quality, higher values were observed for gross calorific value and fixed carbon for Piptadenia stipulacea, while Amburana cearensis exhibited higher levels of volatiles and ash.

https://doi.org/10.1590/1980-509820152505165 1863 downloads

Desenvolvimento infantil: concordância entre a caderneta de saúde da criança e o manual para vigilância do desenvolvimento infantil
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Oliveira, Luddi Luiz de

; Costa, Valmara Maxilaine R.

; Requeijo, Maira R.

; Rebolledo, Rita Santos

; Pimenta, Avani de Fátima

; Lemos, Stela Maris A.

Revista Paulista de Pediatria

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dec 2012, Volume 30 N. 4 Pages 479 - 485

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

OBJETIVO: Verificar a concordância entre os instrumentos Caderneta de Saúde da Criança (CSC) e Manual para Vigilância do Desenvolvimento Infantil no contexto da Atenção Integrada às Doenças Prevalentes na Infância (AIDPI) na avaliação do desenvolvimento infantil em crianças de dois a 24 meses. MÉTODOS: Estudo transversal realizado em uma Unidade Básica de Saúde (UBS) de Belo Horizonte de março de 2009 a março de 2010. Após levantamento do número de crianças adscritas às UBS por meio da análise do cartão-espelho de vacinação, caderno de registro do teste do pezinho e dos dados fornecidos por Agentes Comunitários de Saúde, os responsáveis receberam convite verbal ou por telefone (amostragem por conveniência). Participaram da pesquisa 364 das 623 crianças atendidas no local. O desenvolvimento neuropsicomotor foi avaliado por estudantes graduandos em Enfermagem, Fisioterapia, Fonoaudiologia, Medicina, Nutrição e Terapia Ocupacional. Para análise da concordância dos instrumentos, utilizou-se o coeficiente Kappa. RESULTADOS: A Caderneta de Saúde da Criança classificou 115 crianças (31,6%) com algum atraso no desenvolvimento, enquanto o Manual para Vigilância do Desenvolvimento Infantil no Contexto da AIDPI considerou 124 crianças (34,1%) desta forma. O coeficiente Kappa foi de 0,27. CONCLUSÕES: Verificou-se haver baixa concordância entre os instrumentos analisados.

OBJECTIVE: To assess the extent to which the tools Child Health Handbook and Guide for Monitoring Child Development the context of Integrated Management of Childhood Illness (IMCI) agree for the evaluation of children's development from two to 24 months of age. METHODS: A cross-sectional study was carried out in a Basic Health Unit in Belo Horizonte, from March 2009 to March 2010. After investigating the number of children under the care of the Basic Health Unit by the vaccination card, the register book of newborn tests, and data provided by the Community Health Workers, parents and tutors were invited to participate either verbally or by phone (convenience sample). Among 623 children assisted in the area, 364 were enrolled. Their psychomotor development was evaluated by students of Nursing, Physical Therapy, Speech Therapy, Medical, Nutrition, and Occupational Therapy. To test the agreement between both tools, the Kappa coefficient was used. RESULTS: According to the Child Health Handbook, 115 children (31.6%) were found to have some development delay, while the Guide for Monitoring Child Development found 124 children (34.1%) with the same problem. The Kappa coefficient was 0.27. CONCLUSIONS: The agreement between both tools was poor.

OBJETIVO: Verificar la concordancia entre los instrumentos Libreta de Salud del Niño (CSC, sigla en portugués) y Manual para Vigilancia del Desarrollo Infantil en el Contexto de la Atención Integrada a las Enfermedades Prevalentes en la Infancia (AIDPI, sigla en portugués) en la evaluación del desarrollo infantil en niños de dos a 24 meses de edad. MÉTODOS: Estudio transversal realizado en una Unidad Básica de Salud (UBS) de Belo Horizonte (Minas Gerais, Brasil), entre marzo de 2009 y marzo de 2010. Después de inventariar el número de niños adscritos a la UBS por medio de análisis de la tarjeta de vacunación, del cuaderno de registro del tamizaje neonatal y de datos suministrados por Agentes Comunitarios de Salud (ACS), los responsables recibieron invitación verbal o por teléfono (muestreo por conveniencia). Participaron de la investigación 364 niños de los 623 atendidos en el local. El desarrollo neuropsicomotor fue evaluado por 20 estudiantes graduados en Enfermería, Fisioterapia, Fonoaudiología, Medicina, Nutrición y Terapia Ocupacional. Para el análisis de la concordancia de los instrumentos, se utilizó el coeficiente Kappa. RESULTADOS: La Libreta de Salud del Niño clasificó a 115 niños (31,6%) como presentando algún retraso en el desarrollo, mientras que el Manual para Vigilancia del Desarrollo Infantil en el Contexto de la AIDPI consideró a 124 niños (34,1%) de este modo. El coeficiente Kappa fue 0,27. CONCLUSIONES: Se verificó una baja concordancia entre los instrumentos analizados.

15205 downloads Cited 2 times in SciELO

10.

Squamous cell carcinoma in vaginal fundus in a Brahman cow
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Pimenta-Oliveira, A.

; Oliveira-Filho, J.P.

; Cagnini, D.Q.

; Badial, P.R.

; Cisneros-Álvarez, E.

; Laufer-Amorim, R.

; Prestes, N.C.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2011, Volume 63 N. 3 Pages 749 - 752

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

É descrito o carcinoma de células escamosas (CCE) no fundo vaginal de uma vaca. O diagnóstico de CCE moderadamente diferenciado foi confirmado através do exame histopatológico. Os testes imunoistoquímicos com os marcadores p53 e Ki67 realizados em amostras do tumor confirmaram a mutação na p53 e aumento da proliferação celular.

4470 downloads

11.

The CAG repeat within the androgen receptor gene and its relationship to cryptorchidism
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Silva-Ramos, M.

; Oliveira, J. M.

; Cabeda, J. M.

; Reis, A.

; Soares, J.

; Pimenta, A.

International braz j urol

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jun 2006, Volume 32 N. 3 Pages 330 - 335

Abstract: En | Text: En | PDF: En

PURPOSE: We examined the significance of the CAG repeat polymorphism in the pathogenesis of cryptorchidism. MATERIALS AND METHODS: Genomic deoxyribonucleic acid (DNA) was extracted from blood samples from 42 cryptorchid boys and from 31 non-cryptorchid control subjects. In the cryptorchid group, 7 had bilateral cryptorchidism and 6 had patent processus vaginalis in the contralateral side. To determine the number of CAG repeats, the DNA was amplified by polymerase chain reaction and sequenced. RESULTS: The mean CAG repeat length in the AR gene was 22.5 (range 16 to 28) in patients and 21.5 (range 17 to 26) in controls (non-significant). Patients with bilateral cryptorchidism had a mean length of 24.3 (range 21 to 26) and patients with unilateral cryptorchidism and patent processus vaginalis in the contra lateral side had a mean of 25.2 (range 21 to 28), which was statistically different from controls (p = 0.015 and p = 0.005 respectively). CONCLUSION: CAG repeat length of the AR gene does not seem to play a major role in patients with unilateral cryptorchidism. However, in patients with bilateral undescended testis, a less functional androgen receptor through a longer polyglutamine chain may have a role in its pathogenesis. In the same way, patients with unilateral cryptorchidism a contralateral patent processus vaginalis have longer CAG repeats that might be responsible for a slower testicular descent and incomplete closure of the processus vaginalis.

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12.

Características clínicas e laboratoriais à admissão de 483 crianças e adolescentes com refluxo vésico-ureteral primário: estudo retrospectivo
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Silva, José Maria Penido

; Diniz, José Silvério S.

; Oliveira, Eduardo A.

; Marino, Viviane S. P.

; Pimenta, Mariana R.

; Matos, Carolina C.

; Vieira, Samana B.

Revista da Associação Médica Brasileira

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apr 2004, Volume 50 N. 2 Pages 153 - 157

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVOS: Avaliar retrospectivamente as características clínicas/radiológicas dos pacientes admitidos com refluxo vésico-ureteral primário (RVU) entre 1969-1999. MÉTODOS: Pacientes com RVU primário foram submetidos a um protocolo de avaliação clínica, laboratorial e radiológica e acompanhados longitudinalmente. Na admissão, após controle da bacteriúria e do início da quimioprofilaxia, foram realizados avaliação laboratorial e estudo de imagens do trato urinário. Esta consistiu de uretrocistografia miccional, urografia excretora, cintilografia renal (DMSA) e ultra-sonografia. Para o presente estudo foram utilizados os dados obtidos na admissão. RESULTADOS: Foram admitidos nesse período 483 pacientes. Houve predomínio do sexo feminino (70%) e da cor branca (70%). A média de idade ao diagnóstico foi de 26 meses, sendo que 92,5% das crianças já haviam apresentado infecção urinária. Um grupo de 710 unidades renais foi analisado. A distribuição do grau de refluxo foi a seguinte: grau I (49; 7%); II (254; 36%); III (190; 26%); IV (161; 23%) e V (56; 8%). Um total de 249 unidades renais apresentaram dano renal à admissão. A distribuição quanto à intensidade da lesão foi a seguinte: leve (36%), moderada (34%) e grave (30%). Houve um risco significativo maior de lesão renal grave no sexo masculino (OR = 1,74, IC 95% = 1,2 - 2,5, p = 0,002). CONCLUSÃO: O RVU predominou no sexo feminino e em sua maioria apresentou-se em graus leves a moderados. Contudo, houve um alto percentual de dano renal à admissão e uma tendência de maior morbidade para o sexo masculino.

OBJECTIVES: To evaluate the clinical/radiological features of patients with primary vesicoureteral reflux (VUR) admitted to a single institution from 1969 to 1999. METHODS: At admission, after the institution of chemoprophylaxis, patients were investigated by CUM, DMSA, and US. The children were managed with periodical clinical and laboratory evaluations. Analyses were performed with the data obtained at admission. RESULTS: A total of 483 patients were enrolled in the protocol. There was a predominance of females (70%) and caucasian race (70%). Mean age at VUR diagnosis was 26 months and 92.5% of the patients had urinary infection before admission. A total of 710 refluxing units were analyzed. The distribution of reflux grade was: grade I (49; 7%); II (254; 36%); III (190; 26%); IV (161; 23%) e V (56; 8%). Approximately one half of the units analyzed were from patients presenting renal damage at admission. The distribution of the severity of renal damage was as follows: mild (36%), moderate (34%), and severe (30%). There was a significant risk of severe renal damage for the males (OR = 1.74, 95% CI = 1.2 - 2.5, p = 0.002). CONCLUSION: There was a predominance of VUR among females and most presented a mild or moderate degree. However, there was a high percentage of renal damage at admission and a tendency to greater morbidity for the males.

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13.

Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in in vitro and in vivo assays
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Teixeira, Rosangela de Oliveira

; Camparoto, Marjori Leiva

; Mantovani, Mário Sérgio

; Vicentini, Veronica Elisa Pimenta

Genetics and Molecular Biology

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dec 2003, Volume 26 N. 4 Pages 551 - 555

Abstract: En | Text: En | PDF: En

The use of medicinal plants by the general population is an old and still widespread practice, which makes studies of their genotoxicity essential. Psidium guajava L. and Achillea millefolium L. are examples of plants commonly used in popular medicine. P. guajava L. is indicated for diarrhea and also as an antiseptic, while A. millefolium L. is indicated as an analgesic, antispasmodic, digestive, diuretic, antiseptic, astringent, emollient, wound healer and hemorrhoid medication. The aim of this study was to determine the effects of the infusions of these two plant species on chromosomes and the cell cycle. Leaves from the plants were used to prepare infusions, in the same manner as teas, but at two different concentrations. Allium cepa L. root-tip cells (P. guajava L. - 2.62 and 26.2 mg/mL, and A. millefolium L. - 3.5 and 35.0 mg/mL) and Wistar rat bone marrow cells (P. guajava L. - 2.62 and 26.2 mg/100g body weight, and A. millefolium L. - 3.5 and 35.0 mg/100g body weight) were used as in vivo plant and animal test systems, respectively. Human peripheral blood lymphocytes (P. guajava L. - 0.262 and 2.62 mg/mL culture medium, and A. millefolium L. - 0.35 and 3.5 mg/mL culture medium) were used as in vitro test system. The P. guajava L. infusion at the higher concentration caused a statistically significant inhibition of cellular division in the onion root-tip cells, not observed in onion root-tip cells treated with A. millefolium L. No statistically significant alterations were found, as compared to untreated controls, in either the cell cycle or the number of chromosome alterations, after treatments with either plant, in rat cells or in cultured human lymphocytes. These results regarding the cytotoxicity and mutagenicity of these plants provide valuable information about the safety of using them as therapeutic agents.

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14.

Diretriz de fibrilação atrial
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Martinelli Filho, Martino

; Moreira, Dalmo A. Ribeiro

; Lorga, Adalberto M

; Sosa, Eduardo

; Atié, Jacob

; Pimenta, João

; Andrade, José Carlos S. de

; Fagundes, Márcio Luis A.

; Kuniyoshi, Ricardo Ryoshim

; Lorga Filho, Adalberto

; Lorga, Adalberto Menezes

; Lopes, Alberto Nicodemus G

; Paola, Ângelo A. V. de

; Costa, Álvaro Barros da

; Péres, Ayrton Klier

; Grupi, César J.

; Halperin, Cídio

; Moreira, Dalmo A. Ribeiro

; Sousa, Eduardo A.

; Sternick, Eduardo

; Cruz, Fernando Eugênio S.

; Darrieux, Francisco Carlos C.

; Lima, Gustavo Glotz

; Atié, Jacob

; Jorge, José Carlos Moura

; Andrade, José Carlos S. de

; Mateos, José Carlos Pachón

; Pimenta, João

; Oliveira, Júlio Cesar de

; Zimerman, Leandro I.

; Fagundes, Márcio Luis A.

; Martinelli Filho, Martino

; Scanavacca, Maurício I.

; Medeiros, Paulo de Tarso J.

; Miranda, Reynaldo Castro

; Costa, Roberto

; Kunyioshi, Ricardo Ryoshim

; Rassi, Sérgio Gabriel

; Galvão Filho, Silas S

; Seixas, Tamer N.

; Rodrigues, Thiago da Rocha

; Guimarães, Jorge Ilha

Arquivos Brasileiros de Cardiologia

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nov 2003, Volume 81 Suppl. 6 Pages 2 - 24

Abstract: Pt | Text: Pt | PDF: Pt

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15.

Estudo morfológico de aderências peritoneais induzidas pela rifamicina, em ratos
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D´Oliveira, Djalma Manoel Rodrigues

; Nigro, Amaury José Teixeira

; Fagundes, Djalma José

; Novo, Neil Ferreira

; Juliano, Yara

; Pimenta, Ana Luisa Pinheiro

Acta Cirúrgica Brasileira

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jun 2001, Volume 16 N. 2 Pages 103 - 109

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O uso da rifamicina sv intraperitoneal e a sua influência na formação de aderências peritoneais pós-operatórias (APs), foram analisados num estudo experimental em 90 ratos, machos, da linhagem Wistar-UNIG, distribuídos em dois grupos iguais. O grupo A (n = 45) foi submetido à laparotomia e instilação de 2 mL de solução salina de cloreto de sódio a 0,9%, realizando-se movimento digital rítmico, nos quatro quadrantes da cavidade peritoneal, totalizando um tempo de manuseio de dois minutos. Após este procedimento, a solução remanescente foi aspirada com seringa, seguida de laparorrafia imediata. O grupo B (n = 45), foi submetido a procedimento semelhante com a instilação de uma solução de rifamicina sv 500mg diluída em 500mL de solução salina de cloreto de sódio a 0,9%, utilizando manobra idêntica àquela utilizada no grupo A. Os animais do grupo A e os do grupo B foram separados em três subgrupos com igual número (n = 15) de acordo com o período de eutanásia, que foi de 7, 14 e 21 dias pós-operatório. As aderências peritoneais foram quantificadas nos seus respectivos períodos de eutanásia, utilizando um método descrito por MORENO-EGEA, AGUAYO, ZAMBUDIO e PARRILLA (1993). O grupo B apresentou aderências significantemente mais intensas que o grupo A em todos os subgrupos. Esta diferença foi mais marcante nos animais submetidos à eutanásia após 21 dias.

The intraperitoneal use of rifamycin s.v. and its influence in postoperative peritoneal adhesions occurrence was analysed in a ninety male rats experimental study. The control group was formed by 45 animals subjected to laparotomy and instillation of 2mL of saline solution with rhythmic digital movements in the four quadrants of the peritoneal cavity for 2 minutes. After this procedure, the remaining saline solution was aspirated with a syringe, followed by immediate laparorrhaphy. The experiment group (n=45) was submitted to a similar procedure with instillation of a solution of rifamycin s.v. 500mg diluted in 500mL of a saline vehicle, according to the same movement used in the control group. The animals of both control and experiment groups were distributed in three subgroups with equal number (n=15), according to the euthanasia period, which was 7, 14 and 21 days postoperative. Peritoneal adhesions were quantified in the euthanasia periods, using a method described by MORENO-EGEA, AGUAYO, ZAMBUDIO and PARRILLA (1993). The experiment group presented a rate of adhesion significantly high as compared to the control group in all subgroups. This difference was higher in animals with an euthanasia period of 21 days.

https://doi.org/10.1590/S0102-86502001000200008 2023 downloads

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