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Resumo Fundamento A associação entre o status de saúde cardiovascular ideal ( ideal cardiovascular health ( ICVH) e diagnóstico de fibrilação ou flutter atrial (FFA) foi menos estudado em comparação a outras doenças cardiovasculares. Objetivos Analisar a associação entre o diagnóstico de FFA e métricas e escores de ICVH no Estudo Longitudinal de Saúde do Adulto (ELSA-Brasil). Métodos Este estudo analisou dados de 13141 participantes com dados completos. Os traçados eletrocardiográficos foram codificados de acordo com o Sistema de Minnesota, em um centro de leitura centralizado. As métricas do ICVH (dieta, atividade física, índice de massa corporal, tabagismo, glicemia de jeju, e colesterol total) e escores do ICVH foram calculados conforme proposto pela American Heart Association . Modelos de regressão logística bruta e ajustada foram construídos para analisar associações de métricas e escores do ICVH com diagnóstico de FFA. O nível de significância foi estabelecido em 0,05. Resultados A idade mediana da amostra foi de 55 anos, e 54,4% eram mulheres. Nos modelos ajustados, os escores de ICVH não apresentaram associação significativa com diagnóstico de FFA prevalente [odds ratio (OR):0,96; intervalo de confiança de 95% (IC95%):0,80-1,16; p=0,70). Perfis de pressão arterial ideal (OR:0,33; IC95%:0,1-0,74; p=0,007) e colesterol total ideal (OR:1,88; IC95%:1,19-2,98; p=0,007) foram significativamente associados com o diagnóstico de FFA. Conclusões Não foram identificadas associações significativas entre escores de ICVH global e diagnóstico de FFA após ajuste multivariado em nossas análises, devido, ao menos em parte, às associações antagônicas da FFA com métricas de pressão arterial e de colesterol total do ICVH. Nossos resultados sugerem que estimar a prevenção da FFA por meio de escore de ICVH global pode não ser adequado, e as métricas do ICVH devem ser consideradas separadamente.

Abstract Background The association between ideal cardiovascular health (ICVH) status and atrial fibrillation or flutter (AFF) diagnosis has been less studied compared to other cardiovascular diseases. Objective To analyze the association between AFF diagnosis and ICVH metrics and scores in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Methods This study analyzed data from 13,141 participants with complete data. Electrocardiographic tracings were coded according to the Minnesota Coding System, in a centralized reading center. ICVH metrics (diet, physical activity, body mass index, smoking, blood pressure, fasting plasma glucose, and total cholesterol) and scores were calculated as proposed by the American Heart Association. Crude and adjusted binary logistic regression models were built to analyze the association of ICVH metrics and scores with AFF diagnosis. Significance level was set at 0.05. Results The sample had a median age of 55 years and 54.4% were women. In adjusted models, ICVH scores were not significantly associated with prevalent AFF diagnosis (odds ratio [OR]:0.96; 95% confidence interval [95% CI]:0.80-1.16; p=0.70). Ideal blood pressure (OR:0.33; 95% CI:0.15–0.74; p=0.007) and total cholesterol (OR:1.88; 95% CI:1.19–2.98; p=0.007) profiles were significantly associated with AFF diagnosis. Conclusions No significant associations were identified between global ICVH scores and AFF diagnosis after multivariable adjustment in our analyses, at least partially due to the antagonistic associations of AFF with blood pressure and total cholesterol ICVH metrics. Our results suggest that estimating the prevention of AFF burden using global ICVH scores may not be adequate, and ICVH metrics should be considered in separate.

https://doi.org/10.36660/abc.20210970

New flavone and other compounds from Tephrosia egregia: assessing the cytotoxic effect on human tumor cell lines
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Teixeira, Maria V.S.

; Lima, Jefferson Q.

; Pimenta, Antônia T.A.

; Silva, Francisca R.L. da

; Oliveira, Maria da Conceição F. de

; Pereira, Italo G.

; Costa-Junior, Dilailson C.

; Ribeiro, Paulo R.V.

; Santiago, Gilvandete M.P.

; Lima, Mary Anne S.

; Braz-Filho, Raimundo

; Bezerra, Mirna M.

; Montenegro, Raquel C.

; Rocha, Danilo D.

; Moraes, Maria Elisabete A. de

; Medeiros, Aline C. de

; Arriaga, Angela M.C.

Revista Brasileira de Farmacognosia

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jun 2018, Volume 28 N. 3 Pages 333 - 338

Abstract: En | Text: En | PDF: En

ABSTRACT The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided a new flavone, 5-hydroxy-8-(1",2"-epoxy-3"-hydroxy-3"-methylbutyl)-7-methoxyflavone (1), in addition to eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecansone A, 2',6'-dimethoxy-4',5'-(2",2"-dimethyl)-pyranochalcone, pongachalcone, maackiain, β-sistosterol and its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving comparison with literature data. Cytotoxicity of compounds 1–4 was evaluated against AGP-01 (cancerous ascitic fluid), HCT-116 (colon adenocarcinoma), HL-60 (leukemia), PC-3 (prostate carcinoma), SF-295 (glioblastoma) and SKMEL 28 (melanoma) cell lines.

https://doi.org/10.1016/j.bjp.2018.03.008 525 downloads

ANÁLISES FÍSICO-QUÍMICAS DE BIOFILMES DE SULFATO DE CONDROITOINA MODIFICADO
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Bunhak, Élcio J.

; Mendes, Elisabete S.

; Pereira, Nehemias C.

; Pineda, Edgardo A. G.

; Hechenleitner, Ana A. W.

; Cavalcanti, Osvaldo A.

Química Nova

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mar 2015, Volume 38 N. 3 Pages 316 - 320

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Numerous investigations are dedicated to the research and development of new polymer materials destined for innovation in pharmaceutical forms. The application of these technological resources has allowed the commercialization of new therapeutic systems for modified drug release. This investigation aimed to evaluate the association of modified chondroitin sulfate with an insoluble polymer, Eudragit® RS 30 D, widely available in the pharmaceutical market. Isolated films were prepared by the evaporation process using a Teflon® plate. The aqueous dispersions (4% m/v) of synthetic polymer received the addition of modified chondroitin sulfate at different ratios. The interactions of the polymer chains in the blends were physicochemically characterized by means of Fourier transform infrared spectroscopy, thermal analyses, differential scanning calorimetry, thermogravimetry and scanning electron microscopy combined with hydration and assays in alkaline pH. The results showed appropriate properties of the coating materials for solid oral forms intended for drug deliver in specific environments.

https://doi.org/10.5935/0100-4042.20150030 1734 downloads

HIV AND HCV COINFECTION: PREVALENCE, ASSOCIATED FACTORS AND GENOTYPE CHARACTERIZATION IN THE MIDWEST REGION OF BRAZIL
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Freitas, Solange Zacalusni

; Teles, Sheila Araújo

; Lorenzo, Paulo Cesar

; Puga, Marco Antonio Moreira

; Tanaka, Tayana Serpa Ortiz

; Thomaz, Danilo Yamamoto

; Martins, Regina Maria Bringel

; Druzian, Angelita Fernandes

; Lindenberg, Andréa Siqueira Campos

; Torres, Marina Sawada

; Pereira, Sérgio A.

; Villar, Livia Melo

; Lampe, Elisabete

; Motta-Castro, Ana Rita Coimbra

Revista do Instituto de Medicina Tropical de São Paulo

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dec 2014, Volume 56 N. 6 Pages 517 - 524

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Estudo transversal sobre a prevalência, fatores associados e distribuição dos genótipos do HCV foi realizado em 848 pacientes infectados pelo HIV, recrutados em centros de referência na Região Centro-Oeste do Brasil. A taxa de prevalência de coinfecção HIV-HCV foi de 6,9% (IC 95%: 5,2-8,6). Na análise multivariada, o aumento da idade, o uso de drogas ilícitas (injetáveis e não injetáveis), história de transfusão de sangue antes de 1994, e ausência de companheiro constante foram fatores associados independentes e significativos para a coinfecção HIV-HCV. A análise filogenética baseada na região NS5B revelou a presença de dois principais genótipos do HCV em circulação: genótipos 1 (58,3%) e 3 (41,7%). A prevalência da coinfecção HIV-HCV foi menor do que as relatadas em estudos realizados com pacientes infectados pelo HIV em diferentes regiões do Brasil, devido ao fato de que o uso de drogas ilícitas não é modo frequente de transmissão do HIV neste Estado do Brasil. Triagem sorológica de pacientes HIV-positivos para HCV antes de iniciar o tratamento antirretroviral, identificação completa dos fatores associados e a implementação de programas eficazes de redução de danos são altamente recomendados para fornecer informações úteis, para o tratamento e para evitar a coinfecção com HCV nestes pacientes.

A cross-sectional study on prevalence, associated factors and genotype distribution of HCV infection was conducted among 848 HIV-infected patients recruited at reference centers in the Midwest Region of Brazil. The prevalence rate of HIV-HCV coinfection was 6.9% (95% CI: 5.2 to 8.6). In multivariable analysis, increasing age, use of illicit drugs (injection and non-injection), a history of blood transfusion before 1994, and the absence of a steady partnership were significant independent associated factors for HIV-HCV coinfection. The phylogenetic analysis based on the NS5B region revealed the presence of two major circulating genotypes of HCV: genotypes 1 (58.3%) and 3 (41.7%). The prevalence of HIV-HCV coinfection was lower than those reported in studies conducted with HIV-infected patients in different regions of Brazil, due to the fact that illicit drug use is not a frequent mode of HIV transmission in this region of Brazil. Serologic screening of HIV-patients for HCV before initiating antiretroviral treatment, a comprehensive identification of associated factors, and the implementation of effective harm reduction programs are highly recommended to provide useful information for treatment and to prevent HCV coinfection in these patients.

https://doi.org/10.1590/S0036-46652014000600011 2852 downloads

Formación profesional, básica o general: lo que piensan los estudiantes de una universidad pública del Brasil
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Pereira, Elisabete M. A.

; Vizcarra Herles, Eleonor

; Wassem, Joyce

; Alencar de Caldas, Tania

Actualidades Investigativas en Educación

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aug 2014, Volume 14 N. 2 Pages 566 - 590

Abstract: Es Pt En | Text: Es Pt En | PDF: Es | PDF: Pt | PDF: En

En el contexto de educación superior, la participación de estudiantes en el diseño curricular es poco enfatizada. Este trabajo resulta de una investigación desarrollada con discentes de todas las carreras profesionales de la Universidad Estatal de Campinas - Unicamp - Brasil, con el objetivo de conocer la perspectiva de estos, qué aspectos del currículo de sus carreras profesionales son vistos como esenciales, y cuál sería el principal énfasis curricular que se debería abordar para prepararlos mejor para la actualidad y el futuro mundo del trabajo. Los datos revelan que los estudiantes están hoy más preocupados que en el pasado por cuestiones sociales, y resienten no ver ese énfasis abordado en los currículos. El trabajo es relevante por dar voz a la opinión de los estudiantes, una vez que ejercen un importante papel al transformar el currículo intencionado en currículo experimentado.

Em nosso contexto de educação superior, a participação de estudantes no desenho curricular é pouco enfatizada. Esse trabalho resulta de uma pesquisa desenvolvida com discentes de todos os cursos da Universidade Estadual de Campinas - Unicamp, com o objetivo de conhecer, na perspectiva destes, quais aspectos dos currículos de seus cursos são vistos como essenciais e qual seria a principal ênfase curricular que os currículos deveriam abordar para melhor prepará-los para o atual, e o futuro, mundo do trabalho. Os resultados demonstram uma mudança no comportamento e na visão do aluno sobre qual deva ser a direção de sua formação como profissional, o que desafia as políticas curriculares a tomar em consideração aspectos apontados por eles. Os dados desvelam que os estudantes hoje estão mais preocupados com questões sociais do que estiveram no passado e que se ressentem de não ver essa ênfase abordada nos currículos. A pesquisa é relevante por dar voz à opinião dos estudantes, uma vez que estes exercem importante papel ao transformar o currículo intencionado em currículo experienciado.

In the context of higher education, student participation in curriculum design is little emphasized. This work results from a research developed with students from all courses at the State University of Campinas - Unicamp - Brazil, with the aim of knowing their perspective regarding which curricular aspects of profesional careers are considered to be seen as essential, and which would be the main curricular emphasis that should be applied in order to better prepare them for the current and the future world of work. The data reveal that students today are more concerned with social issues than they have been in the past and resent not see this emphasis addressed in the curriculum. The research is relevant for giving voice to students’ opinion, once they play an important role in transforming the curriculum intended to a experienced curriculum.

1021 downloads

Estratégias de pré-concentração em eletroforese capilar: parte 2. Manipulação da velocidade da fase dispersa/secundária
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Moraes, Maria de Lourdes L. de

; Tavares, Marina F. M.

; Pereira, Elisabete A.

Química Nova

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2010, Volume 33 N. 2 Pages 466 - 470

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

This work describes CE preconcentration strategies based on the effect of manipulation of the disperse/secondary velocity. Introduced by Terabe et al. in 1984, micellar electrokinetic chromatography is a powerful separation approach that increases the usage of electrokinetic phenomena for the separation of nonionic compounds. The main disadvantage of MEKC is the low concentration sensitivity associated with the limited optical path length for on-capillary photometric detection and the limited volume of sample solution that can be injected. This paper compiles on-line concentration strategies for neutral analytes by sample stacking and sweeping in micellar electrokinetic chromatography.

2200 downloads

Development and validation of a capillary electrophoresis method for the determination of sulfate in indinavir sulfate raw material
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Pereira, Elisabete A.

; Micke, Gustavo A.

; Tavares, Marina F. M.

Journal of the Brazilian Chemical Society

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apr 2006, Volume 17 N. 2 Pages 251 - 256

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente trabalho descreve um método simples e rápido por eletroforese capilar, utilizando detecção indireta (230 nm) para determinação quantitativa de sulfato em sulfato de indinavir (matéria-prima). Uma solução contendo 10 mmol L-1 de molibdato de amônio e 0,15 mmol L-1 de brometo de cetiltrimetilamônio (pH 7,5) foi utilizada como eletrólito de corrida. As amostras foram injetadas hidrodinamicamente com pressão 5000 Pa durante 3 s. A resposta foi linear no intervalo entre 10,1 µg mL-1 a 79,8 µg mL-1. Os limites de detecção e quantificação foram de 0,34 e 1,13 µg mL-1, respectivamente. A precisão intra-dia, determinada como desvio padrão relativo, foi de 2,8% para dez preparações independentes da amostra. Os valores de recuperação para três diferentes níveis de concentração variaram de 96,9 a 102,4%. A metodologia foi aplicada a amostras reais. A concentração de sulfato na amostra variou de 13,3 a 13,5% (m/m, calculado com relação ao material seco).

In this work, a simple and fast capillary electrophoresis method using indirect UV detection (230 nm) for the quantitative determination of sulfate in the indinavir sulfate raw material was described. A running electrolyte consisting of 10 mmol L-1 ammonium molybdate containing 0.15 mmol L-1 cetyltrimethylammonium bromide (pH 7.5) was used. Samples were injected hydrodynamically by applying 5000 Pa pressure during 3 s. The response was linear from 10.1 µg mL-1 to 79.8 mg µL-1. Limits of detection and quantification were 0.34 and 1.13 mg µL-1, respectively. The intra-day precision expressed as relative standard deviation was 2.8% for 10 independent sample preparations. Recoveries varying from 96.9 to 102.4% at three concentration levels were obtained. The methodology was successfully applied for real samples. The concentration of sulfate in indinavir sulfate raw material varied from 13.3 to 13.5% (m/m calculated as anhydrous base).

2639 downloads

Avaliação de contaminantes inorgânicos e orgânicos em álcool combustível utilizando eletroforese capilar
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Pereira, Elisabete A.

; Tavares, Marina F. M.

; Stevanato, Alessandra

; Cardoso, Arnaldo A.

Química Nova

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feb 2006, Volume 29 N. 1 Pages 66 - 71

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

This work reports the analysis of inorganic and organic contaminants in alcohol fuel samples using capillary electrophoresis. Chloride and sulfate were analyzed in nitrate/ monochloroacetic acid at 10 mmol L-1 concentration each under indirect UV detection (210 nm). The analysis of aldehydes is based on the 216 nm detection of 3-methyl-2-benzothiazoline hydrazone adducts. The running buffer consisted of 20 mmol L-1 tetraborate , 40 mmol L-1 sodium dodecyl sufate and 12 mmol L-1 beta-ciclodextrin. Both methodologies were applied to real samples indicating inorganic ion concentrations from 0.15 to 6.64 mg kg-1 and aldehydes from 32.0 to 91.3 mg L-1.

2968 downloads

Applications of capillary electrophoresis to the analysis of compounds of clinical, forensic, cosmetological, environmental, nutritional and pharmaceutical importance
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Tavares, Marina F. M.

; Jager, Alessandra V.

; Silva, Clóvis L. da

; Moraes, Edgar P.

; Pereira, Elisabete A.

; Lima, Elizabete C. de

; Fonseca, Fabiana N.

; Tonin, Fernando G.

; Micke, Gustavo A.

; Santos, Márcia R.

; Oliveira, Marcone A. L. de

; Moraes, Maria de Lourdes L. de

; Kampen, Maria Helena van

; Fujiya, Neide M.

Journal of the Brazilian Chemical Society

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apr 2003, Volume 14 N. 2 Pages 281 - 290

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Desde sua implementação na década de 80, a eletroforese capilar vem evoluindo, tornando-se uma técnica de separação bem estabelecida e, hoje, engloba de fato, uma família de técnicas eletrocinéticas, com mecanismos de separação distintos e seletividade característica, todas passíveis de serem conduzidas em uma única coluna capilar. Neste trabalho, a versatilidade da eletroforese capilar para lidar com materiais de diferentes classes químicas e matrizes complexas é ilustrada, apresentando aplicações representativas nas áreas clínica, forense, cosmética, ambiental, nutricional e farmacêutica, compilando os interesses de pesquisa e resultados do nosso próprio grupo.

Since its inception in the 80's, capillary electrophoresis has matured into a well-established separation technique, actually encompassing a family of electrodriven techniques with distinct separation mechanisms and selectivity, performed in a single capillary column. In this work, the versatility of capillary electrophoresis in handling materials from a diversity of chemical classes and complex sample matrices is illustrated by representative applications in the clinical, forensic, cosmetological, environmental, nutritional and pharmaceutical areas, grouping together our own research interests and results.

13432 downloads

10.

Gota suspensa para avaliação de aldeído total no ar interno e externo do ambiente
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Pereira, Elisabete A.

; Cardoso, Arnaldo A.

; Dasgupta, Parnendu K.

Química Nova

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aug 2001, Volume 24 N. 4 Pages 443 - 448

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A sensitive and simple system was proposed for the in situ measurement of total aldehyde in outdoor or indoor ambient. The method is based on the use of a reagent drop as an useful interface to preconcentrate the sample prior to determination of total aldehyde as formaldehyde. The drop is formed at the tip of a cylindrical tube that contains two optical fibers placed on opposite sides and in contact with the reagent solution. One optical fiber carries the red light to the drop form a light emitting diode (LED). The transmitted light is measured by a second optical fiber/photodiode system. The analytical signal is read and converted into absorbance. The reagent solution of 3-methyl-2-benzothiazoline hydrazone (MBTH) forms a blue cation during reaction with formaldehyde that can be measured at 660 nm. Some aspects of kinetics reaction formation of dye were reevaluated. The formaldehyde reacts with MBTH and forms the azine in about 12 min. The oxidation of MBTH by Fe (III) and the formation of dye requires 3 min. The absorbance of the reagent drop is proportional to the sampling time and to the analyte concentration. The absorbance signal increases with increased sample gas flow until a maximum is reached then decreases until it forms a plateau. The proposed method was evaluated using both outdoor and indoor samples, and it was shown to viable provide an accurate measure of total aldehyde.

3077 downloads

11.

Gotas suspensas: uma proposta para amostragem e análise de gases traços da atmosfera
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Cardoso, Arnaldo A.

; Pereira, Elisabete A.

; Rocha, Júlio C.

Química Nova

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apr 1998, Volume 21 N. 2 Pages 217 - 220

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

In the present paper, we report on the analytical use of a dynamic droplet based gas collection and an analysis system. A droplet formed at the tip of a tube represents a sampling approach that provides an indefinitely renewable surface and uses very little reagent. Sample gas flows past the droplet at a low flow rate. After the gas was sampled , the analysis can be carried out by different methodologies. The feasibility of the sensor is demonstrated by continuos determination of gaseous as: NO2, Cl2 and SO2.

3810 downloads

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