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au:Pedro, Karla P.

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Primer registro de distribución para Psorophora varipes (Coquillett, 1904) (Diptera: Culicidae) en Quintana Roo, México
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Canto-Mis, Karla Leticia

; Gómez-Rivera, Ángel S.

; Chan-Chable, Rahuel J.

; González-Acosta, Cassandra

; Correa-Morales, Fabián

; Mis Ávila, Pedro C.

Revista chilena de entomología

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jun 2022, Volume 48 N. 2 Pages 293 - 297

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract The first distribution record of Psorophora varipes in the state of Quintana Roo is presented from entomological collections made in the localities of La Unión, Cocoyol and José N. Rovirosa belonging to the municipality of Othón P. Blanco. With the addition of this new record to the list of Culicidae of Quintana Roo, a total of 88 species is reached.

Resumen Se presenta el primer registro de distribución de Psorophora varipes en el estado de Quintana Roo a partir de colectas entomológicas realizadas en las localidades de La Unión, Cocoyol y José N. Rovirosa pertenecientes al municipio de Othón P. Blanco. Con la adición de este nuevo registro a la lista de Culicidae de Quintana Roo, se alcanza un total de 88 especies.

https://doi.org/10.35249/rche.48.2.22.10

Astaxanthin Recovery from Shrimp Residue by Solvent Ethanol Extraction Using Choline Chloride:Glycerol Deep Eutectic Solvent as Adjuvant
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Santos, Cleidijane A. dos

; Padilha, Carlos E. A.

; Damasceno, Karla S. F. S. C.

; Leite, Pedro I. P.

; Araújo, Ana C. J. de

; Freitas, Priscila R.

; Vieira, Érica A.

; Cordeiro, Angela M. T. M.

; Sousa Jr., Francisco C. de

; Assis, Cristiane F. de

Journal of the Brazilian Chemical Society

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may 2021, Volume 32 N. 5 Pages 1030 - 1039

Abstract: En | Text: En | PDF: En

The present study investigated the use of choline chloride:glycerol deep eutectic solvent as an adjuvant for tuning the ethanol extraction of astaxanthin from Litopenaeus vannamei processing residues. The adjuvant concentration did not show a clear trend on the total carotenoid, however, the addition of adjuvant proved to be advantageous in the ultrasound-assisted experiments. In order to establish a balance between total carotenoid and productivity, an operational condition with ultrasound, 5% (m/v) choline chloride:glycerol and 10 min of incubation was selected, which provided 737.69 µg g-1 total carotenoids and 32.71 µg g-1 astaxanthin. When compared to the astaxanthin standard, the shrimp residue extract obtained greater antioxidant activity in free radical scavenging tests. In addition, the shrimp residue extract dramatically reduced the dosage of norfloxacin (up to 87.5%) and gentamicin (up to 75.0%) antibiotics in tests to inhibit the growth of Gram-negative bacteria.

https://doi.org/10.21577/0103-5053.20210005 173 downloads

Nem todo abscesso em pequenos ruminantes é causado por Corynebacterium pseudotuberculosis
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Silva, Reanne M.M.

; Cerqueira, Robson B.

; Vieira, Vinicius P.

; Ribas, Jorge R.L.

; Nascimento, Karla A.

; Pimentel, Luciano A.

; Pedroso, Pedro Miguel O.

; Macêdo, Juliana T.S.A.

Pesquisa Veterinária Brasileira

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oct 2018, Volume 38 N. 10 Pages 1902 - 1908

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O presente estudo objetivou determinar a prevalência e distribuição de lesões abscedativas, identificação do agente etiológico e avaliação das lesões histológicas em caprinos e ovinos abatidos em um matadouro-frigorífico com Serviço de Inspeção Federal do estado da Bahia. Foram coletadas 153 amostras de vísceras e linfonodos com abscessos de 1.148 animais abatidos. A maior prevalência na espécie ovina foi em macho, com faixa etária de 12 meses, sendo os principais órgãos acometidos fígado (21,2%) e linfonodo pré-escapular (20,3%). Na espécie caprina, a prevalência maior foi em macho, com faixa etária de 30 meses, sendo os linfonodos retro faríngeo (25%) e pré-escapular os mais acometidos (25%). Isolou-se os seguintes micro-organismos das amostras: Corynebacterium pseudotuberculosis em 33,33%, Escherichia coli (19,61%), Proteus mirabilis (9,80%), Pseudomonas aeruginosa (7,19%), Trueperella pyogenes (5,22%), Streptococcusspp. (5,22%) e Staphylococcus aureus (4,57%). As lesões macroscópicas e histológicas dos abscessos coletados não apresentaram diferenças entre micro-organismos isolados.

ABSTRACT: The study aimed to determine the prevalence and distribution of abscessed lesions, etiologic agent identification and assessment of histological lesions in sheep and goats slaughtered in a slaughter plant refrigerator with Federal Inspection Service in the State of Bahia. The amount of 153 samples of viscera and lymph nodes with abscesses of 1.148 slaughtered animals were collected. The highest prevalence in sheep was in males, aged 12 months, as in liver (21.2%) and prescapular lymph nodes (20.3%) the main affected organs. The prevalence in goats in male, aged 30 months and in retropharyngeal (25%) and prescapular lymph nodes (25%). The following microorganisms were isolated from the samples: Corynebacterium pseudotuberculosis 33.33%, Escherichia coli 19.61%, Proteus mirabilis 9.80%, Pseudomonas aeruginosa 7.19%, Trueperella pyogenes 5.22%, Streptococcus spp. 5.22% and Staphylococcus aureus 4.57%. The macroscopic and histological lesions of abscesses collected presented no difference between isolated microorganisms.

https://doi.org/10.1590/1678-5150-pvb-5630 1794 downloads

Diversidad genética de maracuyá en GUATEMALA revelada por marcadores AFLP
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Ponciano-Samayoa, Karla Melina

; Lacán de León, Juan Pedro

Agronomía Mesoamericana

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jun 2012, Volume 23 N. 1 Pages 73 - 80

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Los objetivos de este estudio fueron caracterizar con AFLP nueve genotipos colectados en Guatemala y determinar la diversidad genética existente. En el Instituto de Ciencia y Tecnología Agrícolas (ICTA), en el período julio 2010/mayo 2011, se realizó este análisis preliminar amplificando diez combinaciones selectivas con las que se detectaron 106 polimorfismos. Las combinaciones selectivas E+ACG/M+CAG, E+ACA/M+CTA, E+ACT/M+CTG y E+AAC/M+CTT dieron el mayor grado de información. En promedio se visualizaron diez loci por amplificación selectiva. El análisis de similaridad reveló que los genotipos no están duplicados. Los análisis de correspondencia y conglomerados identificaron dos grupos bien definidos. El primero incluyó a los materiales de P. edulis f. edulis Sims y el segundo a los materiales de P. edulis f. flavicarpa Degener. La diversidad genética de Nei para la colección fue 0,3160. La diferenciación genética (Gst) fue 0,2542. El 25,42% de la diversidad se expresó entre grupos mientras que el 74,58% dentro de estos. Los resultados dan evidencia de la cercanía evolutiva de los tipos amarilla y morada de P. edulis Sims. El flujo genético fue alto (Nm=1,4670) como se esperaba en una especie alógama en la que se favorece el intercambio inter e intraespecífico.

The objectives of this study were to characterize with AFLP nine genotypes of passion fruit collected in Guatemala, to identify clusters and to evaluate the existing genetic diversity. The study was conducted at Instituto de Ciencia y Tecnología Agrícolas (ICTA), during July 2010/May 2011. A preliminary analysis was conducted in which ten selective primer pairs were amplified and 106 polymorphisms were detected. The selective primer pair E+ACG/M+CAG, E+ACA/M+CTA, E+ACT/M+CTG and E+AAC/M+CTT, gave the most information. An average of ten loci per selective amplification was detected. Similarity analysis revealed no duplicated genotypes. Correspondence and clustering analysis identified two groups. The first one included all accession of P. edulis f. edulis Sims and the second one all accessions of P. edulis f. flavicarpa Degener. The genetic diversity based on Nei´s index for the collection was 0,3160. Genetic differentiation (Gst) was 0,2542 for the entire collection; 25.42% of the genetic diversity is expressed between groups while the 74,58% is expressed within groups. Those results give evidence of the evolutionary proximity of the purple and yellow types of P. edulis Sims. The genetic flow was high (Nm=1,4670) as expected in an allogamous species in which genetic exchange is facilitated.

767 downloads Cited 1 time in SciELO

PET-Saúde: uma experiência prática de integração ensino-serviço-comunidade
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Ferreira, Vinicius Santos

; Barreto, Renata Lavigne Moniz

; Oliveira, Emilie Kruschewskt

; Ferreira, Pedro Rafael Farias

; Santos, Laryssa Passos Sarmento

; Marques, Vitor Eduardo Andrade

; Souza, Monaliza Lemos de

; Menezes, Vanessa Valverde

; Soares, Karla Thayse Mendes

; Oliveira, Laís Ribeiro

; Souza, Lorena Marinho de

; Mendes, Rízia de Melo

; Pinto, Edmundo P.

; Bittencourt, Maria

; Tapioca, Silvana

; Almeida, Rita de Cássia S.

; Linhares, Andréa

; Farias, Alessandra

; Santana, Meire Núbia S. de

Revista Brasileira de Educação Médica

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mar 2012, Volume 36 N. 1 Pages 147 - 151

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este trabalho relata a experiência de integração ensino-serviço-comunidade vivenciada por membros de equipes de Saúde da Família e graduandos de Medicina e Enfermagem participantes do PET-Saúde da Universidade Estadual de Santa Cruz. A referida integração contextualizou-se com o desenvolvimento do projeto "Unidos contra a dengue", mobilização conjunta da instituição de ensino com o serviço de saúde e a comunidade no combate à dengue no bairro Nossa Senhora da Vitória, Ilhéus-Bahia. O foco das ações desse projeto foram as atividades de educação em saúde, nas quais se empregou preferencialmente metodologias ativas de ensino e aprendizagem. A mobilização contou com a participação expressiva da população das mais diversas faixas etárias. O sucesso do projeto ressalta a importância da implantação de programas como o PET-Saúde, que fortalecem a interação entre ensino-serviço-comunidade - importante para construção de serviços de saúde mais qualificados, capazes de inter-relacionar promoção, prevenção e assistência à saúde, possibilitando ações nessa área mais próximas das reais necessidades do SUS. Com esta experiência, espera-se ter contribuído para a formação de graduandos em saúde e qualificação em serviço de profissionais dessa área mais dispostos a trabalhar de forma integrada e integradora com a comunidade.

This study reports on a practical experience with the integration between learning, services, and the community including members of family health teams and undergraduate medical and nursing students participating in the Educational Program for Health Work (PET-Saúde) at Santa Cruz State University. The integration took place within the context of the project "United Against Dengue", an integrated mobilization of the teaching institution, health services, and community in the fight against dengue fever in the district of Nossa Senhora da Vitória, Ilhéus, Bahia State. The projects focused on health education activities, especially with active teaching and learning methods. The mobilization included a significant range of age groups. The project's success emphasizes the importance of implementing programs such as PET-Saúde that strengthen the interaction between learning, services, and the community, important for developing more qualified health services with the capacity to interrelate health promotion, prevention, and healthcare, fostering health activities more in line with the needs of the Unified National Health System (SUS). This experience aims to contribute to the qualification and training of health professionals and students that are willing to work in an integrated and integrating approach with the community.

10419 downloads Cited 5 times in SciELO

The hammock: a reservoir of allergens
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Rego, Francisca X. M.

; Giavina-Bianchi, Pedro

; Kalil, Jorge

; Arruda, L. Karla

; Toledo-Barros, Myrthes

Clinics

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2011, Volume 66 N. 7 Pages 1199 - 1202

Abstract: En | Text: En | PDF: En

INTRODUCTION: Asthma affects approximately 10% of the world's population. Sensitization to allergens is an important risk factor, and exposure to allergens is associated with disease severity. METHODS: We performed skin tests to evaluate allergen sensitization to mites, cockroaches, cats, dogs, and molds in 73 asthmatic patients. Enzyme Linked Immunosorbent Assay was used to assay the mite and cockroach allergens found in dust from the bedding, hammocks, bedroom floors, living rooms, and kitchens of 29 patients and 14 controls. RESULTS: Fifty patients (68.5%) had positive skin test responses. There were positive responses to D. pteronyssinus (52.0%), B. tropicalis (53.4%), T. putrescentiae (15.0%), E. maynei (12.3%), L. destructor (8.2%), B. germanica (20.5%), P. americana (21.9%), Felis catus (10.9%), C. herbarium (2.7%), A. alternata (4.1%), and P. notatun (1.3%). The exposure to mite and cockroach allergens was similar in the patients and the controls. The Dermatophagoides pteronyssinus Group 1 levels were highest in the beds and hammocks. The Blattella germanica Group 1 levels were highest in the kitchens, living rooms and hammocks. DISCUSSION: The positive skin tests to mites, cockroaches and cats were consistent with previous studies. D pteronyssinus was the most prevalent home dust mite, and hammocks were a source of allergens. To improve asthma prophylaxis, it is important to determine its association with mite allergen exposure in hammocks.

1776 downloads

Study of the cardiac left atrioventricular valvar complex in water buffaloes (Bubalus bubalis) of the Jafarabadi breed
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Esteves, Patrícia R.

; Araújo, Karla P.C.

; Ambrósio, Carlos E.

; Teixeira, Dulcinéa G.

; Martins, Daniele S.

; Morini, Adriana C.

; Morini Junior, João C.

; Duarte, Caio N.

; Bombonato, Pedro P.

; Miglino, Maria A.

; Mariana, Arani N.B.

Pesquisa Veterinária Brasileira

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oct 2009, Volume 29 N. 10 Pages 852 - 858

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foram estudados os complexos valvares atrioventricular esquerdo de 30 búfalos da raça Jafarabadi, machos e adultos, sem alterações cardíacas, provenientes de abatedouros do Estado do Paraná. Os corações foram examinados em seu terço médio para acesso ao complexo valvar, que foi submetido a estudos de área, número e tipificação de cordas tendíneas. Os resultados demonstram que este complexo é formado por duas cúspides principais e quatro cúspides acessórias, apresentam em sua formação de 2-3 músculos papilares, nos quais se inserem de 10-25 cordas tendíneas, que se fixam em cúspides voltadas para a parede do ventrículo. A área total deste complexo apresenta uma média de 38,56cm² com um mínimo de 24,96cm² e um máximo de 55,54cm². Estatisticamente não há relação entre número de cordas e a área da cúspide onde estas estão inseridas, nem com o número de músculos papilares dos quais elas provem.

Atrioventricular valve complex of 30 Jafarabadi water buffaloes, adult males were studied in this research with no heart diseases. The animals were obtained from a slaughterhouse in Brazilian State of Parana. The hearts were opened at the third portion affording access to the valve complex. The complexes had its area, number and type of tendinous cords submitted to analysis. The results showed that the complex is composed by two cusps and four accessory cusps, two or three papillary muscles in which 10-25 tendinous cords fix on the cusps that face the ventricle wall. The total area of the complex was on average 38.56cm², with a minimum of 24.96cm² and a maximum of 55.54cm². Statistically, no relation between the number of cords and the cusps' area where they are inserted or with the number of papillary muscle where they originated from was observed.

2319 downloads

Cystic fibrosis and neonatal screening
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Rodrigues, Roberta

; Gabetta, Carmen S.

; Pedro, Karla P.

; Valdetaro, Fabio

; Fernandes, Maria I. M.

; Magalhães, Patrícia K. R.

; Januário, José N.

; Maciel, Léa M. Z.

Cadernos de Saúde Pública

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2008, Volume 24 Suppl. 4 Pages s475 - s484

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Aspectos clínicos e diagnósticos da fibrose cística são revistos de modo abrangente, com ênfase na triagem neonatal. Esta revisão sistematizada da literatura envolveu busca de contribuições relevantes nos bancos de dados PubMed e SciELO. Referências sobre fibrose cística existem desde a Idade Média. É a doença hereditária autossômica recessiva mais freqüente em caucasianos (1:2.000 a 3.500). Mais de mil mutações levam à doença, a mais comum: "F508 (prevalência: 70% em caucasianos canadenses, americanos e norte-europeus; de 23 a 55% em brasileiros). O defeito básico ocorre na secreção do íon cloro. Sua triagem é assunto polêmico e apesar de estar disponível há quase três décadas, por meio de diferentes protocolos, poucos programas de abrangência nacional existem. Entretanto, o Centers for Disease Control and Prevention, dos Estados Unidos, afirma que o rastreamento neonatal para fibrose cística é justificado. A falta de um teste específico e a heterogeneidade étnica da população brasileira dificultam sua triagem neonatal.

The clinical and diagnostic aspects of cystic fibrosis have been extensively reviewed, with an emphasis on neonatal screening. This systematic literature review involved a search for relevant contributions in the PubMed and SciELO databases. The first references to cystic fibrosis date to the Middle Ages. Cystic fibrosis is the most frequent autosomal recessive hereditary disease among Caucasians (1:2,000 to 3,500). More than 1,000 mutations lead to the disease, the most common being "F508, with 70% prevalence among Canadian, Northern European, and American Caucasians and 23 to 55% prevalence among Brazilians. The basic defect is in chloride ion secretion. Cystic fibrosis screening has long been controversial, and after almost three decades, there are few nationwide programs (most are regional or local). However, the U.S. Centers for Disease Control and Prevention (CDC) has concluded that screening for cystic fibrosis is justified. The lack of a specific screening test and the ethnic heterogeneity of the Brazilian population pose challenges for neonatal screening.

2523 downloads Cited 3 times in SciELO

Cystic fibrosis and neonatal screening
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Rodrigues, Roberta

; Gabetta, Carmen S.

; Pedro, Karla P.

; Valdetaro, Fabio

; Fernandes, Maria I. M.

; Magalhães, Patrícia K. R.

; Januário, José N.

; Maciel, Léa M. Z.

Cadernos de Saúde Pública

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2008, Volume 24 Suppl. 4 Pages s475 - s484

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

5014 downloads Cited 3 times in SciELO

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